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Home / Equine Research Bank / Animals (Basel) / Effects of Topically Applied Betulinic Acid and NVX-207 on M...
Animals : an open access journal from MDPI2021; 11(11); 3250; doi: 10.3390/ani11113250

Effects of Topically Applied Betulinic Acid and NVX-207 on Melanocytic Tumors in 18 Horses.

Abstract: The naturally occurring betulinic acid (BA) and its derivative NVX-207 induce apoptosis in equine melanoma cells in vitro. After topical application, high concentrations of the substances can be reached in healthy equine skin. With the aim to investigate the effect and safety of topically applied BA and NVX-207 in horses with melanocytic tumors, the longitudinal, prospective, randomized, double-blind, placebo-controlled study protocol included eighteen Lipizzaner mares with early-stage cutaneous melanoma assigned to three groups. Melanocytic lesions were topically treated either with a placebo, 1% BA or 1% NVX-207 twice a day for 91 days. Caliper measurements, clinical examinations and blood tests were performed to assess the effects and safety of the medication. The topical treatment was convenient and safe. The volumes of tumors treated with BA were significantly reduced over time as compared to tumors treated with the placebo from day 80 of the study. Although treatment with NVX-207 seemed to decrease tumor volume, these results did not reach statistical significance. The findings must be regarded as preliminary due to the limited group size and need to be replicated in a larger cohort with modified pharmaceutical test formulations. Accordingly, the treatment protocol cannot yet be recommended in its current form.
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Publication Date: 2021-11-13 PubMed ID: 34827981PubMed Central: PMC8614291DOI: 10.3390/ani11113250Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the impacts of applying betulinic acid (BA) and its derivative NVX-207 topically on horses with melanocytic tumors. The study found that BA significantly reduced the volume of tumors over time, while the effects of NVX-207 were not statistically significant.

Overview of the Research

  • The research focuses on testing the effectiveness and safety of topically applied betulinic acid (BA) and its derivative NVX-207 in treating melanocytic tumors in horses.
  • The substances, BA and NVX-207, have been identified to induce apoptosis, or cell death, in equine melanoma cells in vitro, meaning under a controlled laboratory environment.
  • However, this research attempts to study their effects in a real-world scenario where these substances are applied topically to the skin of horses.

Research Methodology

  • The study included eighteen Lipizzaner mares with early-stage cutaneous melanoma, making it a specific and controlled study group.
  • These horses were divided into three groups and were treated with a placebo, 1% BA or 1% NVX-207 applied twice daily for a period of 91 days.
  • The study was longitudinal, meaning it studied the same subjects over this protracted period.
  • The research is also described as prospective, randomized, double-blind, and placebo-controlled, indicating a high level of scientific rigor and control over variables.

Findings of the Study

  • The topical treatment of melanocytic lesions with BA and NVX-207 was found to be convenient and safe, indicating no severe side effects or practical difficulties.
  • The volumes of tumors treated with BA significantly reduced over time when compared to those treated with a placebo from the 80th day of the study onwards.
  • The treatment with NVX-207 seemed to decrease tumor volume but the results were not statistically significant, suggesting that the difference could be due to chance rather than the treatment itself.

Limitations and Recommendations

  • While the findings are promising, they are considered preliminary due to the limited size of the test group.
  • Replication of the study in a larger cohort and with modified pharmaceutical test formulations is recommended to validate and refine the findings.
  • The current form of the treatment protocol, therefore, cannot yet be recommended, indicating further research is needed before such treatments can become standard practice.

Cite This Article

APA
Weber LA, Delarocque J, Feige K, Kietzmann M, Kalbitz J, Meißner J, Paschke R, Cavalleri JV. (2021). Effects of Topically Applied Betulinic Acid and NVX-207 on Melanocytic Tumors in 18 Horses. Animals (Basel), 11(11), 3250. https://doi.org/10.3390/ani11113250

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 11
PII: 3250

Researcher Affiliations

Weber, Lisa A
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany.
Delarocque, Julien
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany.
Feige, Karsten
  • Clinic for Horses, University of Veterinary Medicine Hannover, Foundation, Bünteweg 9, 30559 Hannover, Germany.
Kietzmann, Manfred
  • Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany.
Kalbitz, Jutta
  • Biosolutions Halle GmbH, Weinbergweg 22, 06120 Halle (Saale), Germany.
Meißner, Jessica
  • Department of Pharmacology, Toxicology and Pharmacy, University of Veterinary Medicine Hannover, Foundation, Bünteweg 17, 30559 Hannover, Germany.
Paschke, Reinhard
  • Biozentrum, Martin-Luther-University Halle-Wittenberg, Weinbergweg 22, 06120 Halle (Saale), Germany.
Cavalleri, Jessika-M V
  • Equine Internal Medicine, University Equine Clinic, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria.

Grant Funding

  • 16KN051526 BMWI / Central Innovation Program of the German Federal Ministry for Economic Affairs and Energy

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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