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Home / Equine Research Bank / Am J Vet Res / Isolation and characterization of bone marrow-derived equine...
American journal of veterinary research2007; 68(10); 1095-1105; doi: 10.2460/ajvr.68.10.1095

Isolation and characterization of bone marrow-derived equine mesenchymal stem cells.

Abstract: To isolate and characterize bone marrow-derived equine mesenchymal stem cells (MSCs) for possible future therapeutic applications in horses. Methods: Equine MSCs were isolated from bone marrow aspirates obtained from the sternum of 30 donor horses. Methods: Cells were cultured in medium (alpha-minimum essential medium) with a fetal calf serum content of 20%. Equine MSC features were analyzed to determine selfrenewing and differentiation capacity. For potential therapeutic applications, the migratory potential of equine MSCs was determined. An adenoviral vector was used to determine the transduction rate of equine MSCs. Results: Equine MSCs can be culture-expanded. Equine MSCs undergo cryopreservation in liquid nitrogen without altering morphologic characteristics. Furthermore, equine MSCs maintain their ability to proliferate and differentiate after thawing. Immunocytochemically, the expression of the stem cell marker CD90 can be detected on equine MSCs. The multilineage differentiation potential of equine MSCs was revealed by their ability to undergo adipogenic, osteogenic, and chondrogenic differentiation. Conclusions: Our data indicate that bone marrow-derived stromal cells of horses can be characterized as MSCs. Equine MSCs have a high transduction rate and migratory potential and adapt to scaffold material in culture. As an autologous cell population, equine MSCs can be regarded as a promising cell population for tissue engineering in lesions of the musculoskeletal system in horses.
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Publication Date: 2007-10-06 PubMed ID: 17916017DOI: 10.2460/ajvr.68.10.1095Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the isolation and characterization of bone marrow-derived equine mesenchymal stem cells (MSCs) for potential future therapeutic applications in horses.

Methods

  • The researchers obtained bone marrow aspirates from the sternum of 30 donor horses for the purpose of extracting mesenchymal stem cells.
  • The cells were then cultured in a medium known as alpha-minimum essential medium, which consisted of 20% fetal calf serum.
  • The researchers examined the properties of these MSCs to understand their capacity for self-renewing and differentiation.
  • The migratory potential of these stem cells was also investigated in terms of their potential therapeutic applications.
  • An adenoviral vector was deployed to figure out the transduction rate of equine MSCs.

Results

  • The research found that equine MSCs can be culture-expanded, meaning that they can grow in a culture medium.
  • These MSCs can undergo cryopreservation, a freezing process used to save cells, in liquid nitrogen without any changes to their morphologic characteristics.
  • Upon thawing, equine MSCs can maintain their ability to proliferate and differentiate.
  • Tests identified the presence of a specific stem cell marker CD90 on the equine MSCs, through immunocytochemistry, a testing process that uses antibodies to visualize proteins.
  • The tests showed equine MSCs have the capability to differentiate into several types of cells — they demonstrated adipogenic (fat), osteogenic (bone), and chondrogenic (cartilage) differentiation.

Conclusions

  • The researchers concluded that bone marrow-derived stromal cells of horses can be defined as MSCs based on their characteristics.
  • They also found that equine MSCs have a high transduction rate, meaning they could be engineered for specific applications, and a high migratory potential, enabling them to move around the body.
  • Lastly, the researchers noted that equine MSCs can adapt to scaffold material in culture, a property potentially very valuable for tissue engineering.
  • Given their properties and the fact that they are an autologous cell population (originating from the same organism), equine MSCs are seen as a promising cell population for repairing damage to the musculoskeletal system in horses.

Cite This Article

APA
Arnhold SJ, Goletz I, Klein H, Stumpf G, Beluche LA, Rohde C, Addicks K, Litzke LF. (2007). Isolation and characterization of bone marrow-derived equine mesenchymal stem cells. Am J Vet Res, 68(10), 1095-1105. https://doi.org/10.2460/ajvr.68.10.1095

Publication

American journal of veterinary research
ISSN: 0002-9645
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 68
Issue: 10
Pages: 1095-1105

Researcher Affiliations

Arnhold, Stefan J
  • Department of Anatomy, University of Cologne, Josef-Stelzmann Str 9, 50931 Köln, Germany.
Goletz, Iris
    Klein, Helmut
      Stumpf, Gerald
        Beluche, Lisa A
          Rohde, Carsten
            Addicks, Klaus
              Litzke, Lutz F

                MeSH Terms

                • Adipocytes / cytology
                • Animals
                • Bone Marrow Cells / cytology
                • Cell Differentiation
                • Cell Division
                • Chondrocytes / cytology
                • Female
                • Horses
                • Kinetics
                • Male
                • Mesenchymal Stem Cells / cytology
                • Mesenchymal Stem Cells / physiology
                • Osteogenesis

                Citations

                This article has been cited 44 times.
                1. Luo W, Geng Y, Gao M, Cao M, Wang J, Yang J, Sun C, Yan X. Isolation and Identification of Bone Marrow Mesenchymal Stem Cells from Forest Musk Deer.. Animals (Basel) 2022 Dec 20;13(1).
                  doi: 10.3390/ani13010017pubmed: 36611625google scholar: lookup
                2. Leisengang S, Heilen LB, Klymiuk MC, Nürnberger F, Ott D, Wolf-Hofmann K, Gerstberger R, Rummel C, Schmidt MJ, Arnhold S, Roth J. Neuroinflammation in Primary Cultures of the Rat Spinal Dorsal Horn Is Attenuated in the Presence of Adipose Tissue-Derived Medicinal Signalling Cells (AdMSCs) in a Co-cultivation Model.. Mol Neurobiol 2022 Jan;59(1):475-494.
                  doi: 10.1007/s12035-021-02601-9pubmed: 34716556google scholar: lookup
                3. Fülber J, Agreste FR, Seidel SRT, Sotelo EDP, Barbosa ÂP, Michelacci YM, Baccarin RYA. Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system.. World J Stem Cells 2021 Jun 26;13(6):645-658.
                  doi: 10.4252/wjsc.v13.i6.645pubmed: 34249233google scholar: lookup
                4. Pezzanite L, Chow L, Griffenhagen G, Dow S, Goodrich L. Impact of Three Different Serum Sources on Functional Properties of Equine Mesenchymal Stromal Cells.. Front Vet Sci 2021;8:634064.
                  doi: 10.3389/fvets.2021.634064pubmed: 33996964google scholar: lookup
                5. Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
                  doi: 10.3390/ani11010234pubmed: 33477808google scholar: lookup
                6. MacDonald ES, Barrett JG. The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses.. Front Vet Sci 2019;6:507.
                  doi: 10.3389/fvets.2019.00507pubmed: 32039250google scholar: lookup
                7. Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders.. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
                  doi: 10.1007/s12015-019-09932-0pubmed: 31797146google scholar: lookup
                8. Arnhold S, Elashry MI, Klymiuk MC, Geburek F. Investigation of stemness and multipotency of equine adipose-derived mesenchymal stem cells (ASCs) from different fat sources in comparison with lipoma.. Stem Cell Res Ther 2019 Oct 22;10(1):309.
                  doi: 10.1186/s13287-019-1429-0pubmed: 31640774google scholar: lookup
                9. Yamasaki A, Omura T, Murata D, Kobayashi M, Sunaga T, Kusano K, Ueno Y, Kuramoto T, Hobo S, Misumi K. A pilot study of regenerative therapy by implanting synovium-derived mesenchymal stromal cells in equine osteochondral defect models.. J Equine Sci 2018 Dec;29(4):117-122.
                  doi: 10.1294/jes.29.117pubmed: 30607136google scholar: lookup
                10. Rink BE, Beyer T, French HM, Watson E, Aurich C, Donadeu FX. The Fate of Autologous Endometrial Mesenchymal Stromal Cells After Application in the Healthy Equine Uterus.. Stem Cells Dev 2018 Aug 1;27(15):1046-1052.
                  doi: 10.1089/scd.2018.0056pubmed: 29790424google scholar: lookup
                11. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know.. Front Vet Sci 2018;5:70.
                  doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
                12. Olivera R, Moro LN, Jordan R, Pallarols N, Guglielminetti A, Luzzani C, Miriuka SG, Vichera G. Bone marrow mesenchymal stem cells as nuclear donors improve viability and health of cloned horses.. Stem Cells Cloning 2018;11:13-22.
                  doi: 10.2147/SCCAA.S151763pubmed: 29497320google scholar: lookup
                13. Schröck C, Eydt C, Geburek F, Kaiser L, Päbst F, Burk J, Pfarrer C, Staszyk C. Bone marrow-derived multipotent mesenchymal stromal cells from horses after euthanasia.. Vet Med Sci 2017 Nov;3(4):239-251.
                  doi: 10.1002/vms3.74pubmed: 29152317google scholar: lookup
                14. Eydt C, Geburek F, Schröck C, Hambruch N, Rohn K, Pfarrer C, Staszyk C. Sternal bone marrow derived equine multipotent mesenchymal stromal cells (MSCs): investigations considering the sampling site and the use of different culture media.. Vet Med Sci 2016 Aug;2(3):200-210.
                  doi: 10.1002/vms3.36pubmed: 29067195google scholar: lookup
                15. Bearden RN, Huggins SS, Cummings KJ, Smith R, Gregory CA, Saunders WB. In-vitro characterization of canine multipotent stromal cells isolated from synovium, bone marrow, and adipose tissue: a donor-matched comparative study.. Stem Cell Res Ther 2017 Oct 3;8(1):218.
                  doi: 10.1186/s13287-017-0639-6pubmed: 28974260google scholar: lookup
                16. Rink BE, Amilon KR, Esteves CL, French HM, Watson E, Aurich C, Donadeu FX. Isolation and characterization of equine endometrial mesenchymal stromal cells.. Stem Cell Res Ther 2017 Jul 12;8(1):166.
                  doi: 10.1186/s13287-017-0616-0pubmed: 28701175google scholar: lookup
                17. Branly T, Bertoni L, Contentin R, Rakic R, Gomez-Leduc T, Desancé M, Hervieu M, Legendre F, Jacquet S, Audigié F, Denoix JM, Demoor M, Galéra P. Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-ß1.. Stem Cell Rev Rep 2017 Oct;13(5):611-630.
                  doi: 10.1007/s12015-017-9748-ypubmed: 28597211google scholar: lookup
                18. Tidd N, Michelsen J, Hilbert B, Quinn JC. Minicircle Mediated Gene Delivery to Canine and Equine Mesenchymal Stem Cells.. Int J Mol Sci 2017 Apr 12;18(4).
                  doi: 10.3390/ijms18040819pubmed: 28417917google scholar: lookup
                19. Zayed M, Caniglia C, Misk N, Dhar MS. Donor-Matched Comparison of Chondrogenic Potential of Equine Bone Marrow- and Synovial Fluid-Derived Mesenchymal Stem Cells: Implications for Cartilage Tissue Regeneration.. Front Vet Sci 2016;3:121.
                  doi: 10.3389/fvets.2016.00121pubmed: 28149840google scholar: lookup
                20. Olivera R, Moro LN, Jordan R, Luzzani C, Miriuka S, Radrizzani M, Donadeu FX, Vichera G. In Vitro and In Vivo Development of Horse Cloned Embryos Generated with iPSCs, Mesenchymal Stromal Cells and Fetal or Adult Fibroblasts as Nuclear Donors.. PLoS One 2016;11(10):e0164049.
                  doi: 10.1371/journal.pone.0164049pubmed: 27732616google scholar: lookup
                21. Cruz Villagrán C, Schumacher J, Donnell R, Dhar MS. A Novel Model for Acute Peripheral Nerve Injury in the Horse and Evaluation of the Effect of Mesenchymal Stromal Cells Applied In Situ on Nerve Regeneration: A Preliminary Study.. Front Vet Sci 2016;3:80.
                  doi: 10.3389/fvets.2016.00080pubmed: 27695697google scholar: lookup
                22. Sullivan MO, Gordon-Evans WJ, Fredericks LP, Kiefer K, Conzemius MG, Griffon DJ. Comparison of Mesenchymal Stem Cell Surface Markers from Bone Marrow Aspirates and Adipose Stromal Vascular Fraction Sites.. Front Vet Sci 2015;2:82.
                  doi: 10.3389/fvets.2015.00082pubmed: 26835460google scholar: lookup
                23. Lombana KG, Goodrich LR, Phillips JN, Kisiday JD, Ruple-Czerniak A, McIlwraith CW. An Investigation of Equine Mesenchymal Stem Cell Characteristics from Different Harvest Sites: More Similar Than Not.. Front Vet Sci 2015;2:67.
                  doi: 10.3389/fvets.2015.00067pubmed: 26664993google scholar: lookup
                24. Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.. PLoS One 2015;10(4):e0122954.
                  doi: 10.1371/journal.pone.0122954pubmed: 25902064google scholar: lookup
                25. Maia L, da Cruz Landim-Alvarenga F, Taffarel MO, de Moraes CN, Machado GF, Melo GD, Amorim RM. Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.. BMC Vet Res 2015 Mar 15;11:63.
                  doi: 10.1186/s12917-015-0361-5pubmed: 25879519google scholar: lookup
                26. Cruz Villagrán C, Amelse L, Neilsen N, Dunlap J, Dhar M. Differentiation of equine mesenchymal stromal cells into cells of neural lineage: potential for clinical applications.. Stem Cells Int 2014;2014:891518.
                  doi: 10.1155/2014/891518pubmed: 25506367google scholar: lookup
                27. Rutigliano L, Corradetti B, Valentini L, Bizzaro D, Meucci A, Cremonesi F, Lange-Consiglio A. Molecular characterization and in vitro differentiation of feline progenitor-like amniotic epithelial cells.. Stem Cell Res Ther 2013 Oct 30;4(5):133.
                  doi: 10.1186/scrt344pubmed: 24405576google scholar: lookup
                28. Guest DJ, Ousey JC, Smith MR. Defining the expression of marker genes in equine mesenchymal stromal cells.. Stem Cells Cloning 2008;1:1-9.
                  doi: 10.2147/sccaa.s3824pubmed: 24198500google scholar: lookup
                29. Glynn ER, Londono AS, Zinn SA, Hoagland TA, Govoni KE. Culture conditions for equine bone marrow mesenchymal stem cells and expression of key transcription factors during their differentiation into osteoblasts.. J Anim Sci Biotechnol 2013 Oct 29;4(1):40.
                  doi: 10.1186/2049-1891-4-40pubmed: 24169030google scholar: lookup
                30. Kang JG, Park SB, Seo MS, Kim HS, Chae JS, Kang KS. Characterization and clinical application of mesenchymal stem cells from equine umbilical cord blood.. J Vet Sci 2013;14(3):367-71.
                  doi: 10.4142/jvs.2013.14.3.367pubmed: 23820166google scholar: lookup
                31. Wee AS, Lim CK, Merican AM, Ahmad TS, Kamarul T. Total cell pooling in vitro: an effective isolation method for bone marrow-derived multipotent stromal cells.. In Vitro Cell Dev Biol Anim 2013 Jun;49(6):424-32.
                  doi: 10.1007/s11626-013-9626-0pubmed: 23708918google scholar: lookup
                32. Seo MS, Park SB, Kim HS, Kang JG, Chae JS, Kang KS. Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells.. J Vet Sci 2013;14(2):151-9.
                  doi: 10.4142/jvs.2013.14.2.151pubmed: 23388430google scholar: lookup
                33. Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().. Cell Med 2012;4(1):1-11.
                  doi: 10.3727/215517912X647217pubmed: 23152950google scholar: lookup
                34. Coli A, Nocchi F, Lamanna R, Iorio M, Lapi S, Urciuoli P, Scatena F, Giannessi E, Stornelli MR, Passeri S. Isolation and characterization of equine amnion mesenchymal stem cells.. Cell Biol Int Rep (2010) 2011 Sep 13;18(1):e00011.
                  doi: 10.1042/CBR20110004pubmed: 23124164google scholar: lookup
                35. Ranera B, Remacha AR, Álvarez-Arguedas S, Romero A, Vázquez FJ, Zaragoza P, Martín-Burriel I, Rodellar C. Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue.. BMC Vet Res 2012 Aug 22;8:142.
                  doi: 10.1186/1746-6148-8-142pubmed: 22913590google scholar: lookup
                36. Reich CM, Raabe O, Wenisch S, Bridger PS, Kramer M, Arnhold S. Isolation, culture and chondrogenic differentiation of canine adipose tissue- and bone marrow-derived mesenchymal stem cells--a comparative study.. Vet Res Commun 2012 Jun;36(2):139-48.
                  doi: 10.1007/s11259-012-9523-0pubmed: 22392598google scholar: lookup
                37. Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.. BMC Vet Res 2011 Aug 2;7:42.
                  doi: 10.1186/1746-6148-7-42pubmed: 21810270google scholar: lookup
                38. Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells.. Vet Res Commun 2011 Aug;35(6):355-65.
                  doi: 10.1007/s11259-011-9480-zpubmed: 21614641google scholar: lookup
                39. Raabe O, Reich C, Wenisch S, Hild A, Burg-Roderfeld M, Siebert HC, Arnhold S. Hydrolyzed fish collagen induced chondrogenic differentiation of equine adipose tissue-derived stromal cells.. Histochem Cell Biol 2010 Dec;134(6):545-54.
                  doi: 10.1007/s00418-010-0760-4pubmed: 21076963google scholar: lookup
                40. Wang S, Zhou Y, Seavey CN, Singh AK, Xu X, Hunt T, Hoyt RF Jr, Horvath KA. Rapid and dynamic alterations of gene expression profiles of adult porcine bone marrow-derived stem cell in response to hypoxia.. Stem Cell Res 2010 Mar;4(2):117-28.
                  doi: 10.1016/j.scr.2009.12.002pubmed: 20172499google scholar: lookup
                41. Colleoni S, Bottani E, Tessaro I, Mari G, Merlo B, Romagnoli N, Spadari A, Galli C, Lazzari G. Isolation, growth and differentiation of equine mesenchymal stem cells: effect of donor, source, amount of tissue and supplementation with basic fibroblast growth factor.. Vet Res Commun 2009 Dec;33(8):811-21.
                  doi: 10.1007/s11259-009-9229-0pubmed: 19472068google scholar: lookup
                42. Koch TG, Berg LC, Betts DH. Current and future regenerative medicine - principles, concepts, and therapeutic use of stem cell therapy and tissue engineering in equine medicine.. Can Vet J 2009 Feb;50(2):155-65.
                  pubmed: 19412395
                43. Vidal MA, Robinson SO, Lopez MJ, Paulsen DB, Borkhsenious O, Johnson JR, Moore RM, Gimble JM. Comparison of chondrogenic potential in equine mesenchymal stromal cells derived from adipose tissue and bone marrow.. Vet Surg 2008 Dec;37(8):713-24.
                  doi: 10.1111/j.1532-950X.2008.00462.xpubmed: 19121166google scholar: lookup
                44. Kamishina H, Farese JP, Storm JA, Cheeseman JA, Clemmons RM. The frequency, growth kinetics, and osteogenic/adipogenic differentiation properties of canine bone marrow stromal cells.. In Vitro Cell Dev Biol Anim 2008 Nov-Dec;44(10):472-9.
                  doi: 10.1007/s11626-008-9137-6pubmed: 18716851google scholar: lookup
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