Abstract: Force plates (FP) are considered the gold standard for quantitative equine gait evaluation but are limited to experimental use. An instrumented hoof boot (IHB) with pressure sensors and an inertial measurement unit would allow clinicians to objectively assess kinetic parameters and gait symmetry. Objective: To validate the IHB by comparing kinetic parameters with obtained FP data. Methods: In vivo experiments. Methods: Twenty-seven horses were measured with IHBs on FPs at walk and trot. Mean value kinetic parameters recorded were: (1) peak vertical force (PVF) [N/kg]/peak vertical pressure (PVP) [N/kg]; (2) vertical impulse (AUC) [Nms/kg]; (3) peak loading rate (PLR) [N/ms]; (4) stance duration (StD) [ms] and (5) time of peak vertical force (TPVF) [%stance]/time of peak vertical pressure (TPVP) [%stance]. Agreement and correlation were assessed using Bland-Altman analysis and Kendall's correlation coefficient τ. Significance level was set at α = 5%. Results: Bland-Altman plots showed an overall moderate to good agreement with following mean difference (bias) and 95% limits of agreement (95%LoA) for IHB and FP: PVF/PVP [N/kg] walk: bias -0.197 (95%LoA: -0.786 to 0.392), trot: bias 0.0516 (95%LoA: -1.01 to 1.11), AUC [Nms/kg] walk: bias -80 (95%LoA: -420 to 260), trot: bias -44.4 (95%LoA: -291 to 202), PLR [N/ms] walk: bias 0.467 (95%LoA: -0.582 to 1.52), trot: bias 6.24 (95%LoA: -0.675 to 13.2), StD [ms] walk: bias 60.4 (95%LoA: -6.36 to 127), trot: bias 28.7 (95%LoA: -1.47 to 58.8), TPVF/TPVP [%stance] walk: bias -1.72 (95%LoA: -8.28 to 4.83), trot: bias -2.8 (95%LoA: -6.43 to 0.832). Correlations were very strong to fair positive. Conclusions: Small, mixed-breed population, pending repeatability study, ex vivo calibration on manufacturers' behalf. Conclusions: The IHB is a portable, quick method for kinetic gait analysis that approximates the gold standard FP, offering a promising tool for future clinical use.
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Overview
This research validated a new instrumented hoof boot (IHB) equipped with pressure sensors by comparing its measurements to those from conventional force plates (FP), the gold standard for assessing horse gait kinetics.
The study found that the IHB closely approximates FP data during walking and trotting, suggesting it is a practical tool for clinical kinetic gait evaluation in horses.
Background and Motivation
Force plates (FP) provide precise kinetic data during equine gait analysis but are restricted to laboratory or experimental settings due to their size, cost, and setup complexity.
Clinicians need portable and practical devices to objectively measure kinetic parameters and symmetry in horse gaits during routine evaluations.
The instrumented hoof boot (IHB) integrates pressure sensors and an inertial measurement unit directly into a wearable boot, potentially enabling on-site gait analysis.
Objective
The main goal was to validate the IHB by assessing how well its kinetic measurements match those obtained simultaneously from force plates during walking and trotting.
This ensures that the portable boot can reliably approximate the gold standard data in clinical or field conditions.
Methodology
Subjects: 27 horses of mixed breeds.
Procedure:
Horses were outfitted with IHBs on their hooves while walking and trotting over force plates.
Kinetic parameters were recorded by both systems simultaneously to enable direct comparison.
Measured kinetic parameters included:
Peak Vertical Force (PVF) and Peak Vertical Pressure (PVP) normalized by body weight [N/kg]
Vertical Impulse (Area Under Curve, AUC) [Nms/kg]
Peak Loading Rate (PLR) [N/ms]
Stance Duration (StD) [ms]
Time of Peak Vertical Force/Pressure (TPVF/TPVP) expressed as a percentage of stance phase [% stance]
Data analysis:
Agreement between IHB and FP was assessed with Bland-Altman plots, which show bias and limits of agreement.
Correlations were analyzed using Kendall’s tau correlation coefficient (τ) to assess the strength and direction of association.
A significance level of 5% (α=0.05) was set for statistical tests.
Results
Bland-Altman Analysis:
Generally showed moderate to good agreement across parameters during walk and trot.
Bias values for parameters like PVF/PVP were close to zero, confirming small systematic differences between devices.
95% Limits of Agreement (LoA) indicated variability ranges within acceptable bounds for clinical use, though wider for some measures like vertical impulse.
Kendall’s Tau Correlations:
Correlations between IHB and FP measurements ranged from fair to very strong positive, confirming that both devices tracked similar kinetic patterns.
Correlations were consistent for both walking and trotting gaits.
Conclusions
The instrumented hoof boot provides a portable, rapid method for kinetic gait analysis that reasonably approximates force plate data.
This positions the IHB as a valuable tool for clinical environments where standard force plates are impractical.
Study limitations noted include the small and mixed-breed sample size and the need for further studies on repeatability and ex vivo calibration to confirm accuracy and reliability.
Overall, the IHB offers promise for more accessible, objective assessment of horse locomotion in clinical and field conditions.
Cite This Article
APA
Keller J, Hassenstein MJ, Jung K, Geburek F.
(2026).
Validation of a novel instrumented hoof boot with pressure sensors using force plate gait kinetics in horses at walk and trot.
Equine Vet J.
https://doi.org/10.1002/evj.70332
Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany.
Hassenstein, Max J
Institute of Animal Genomics, University of Veterinary Medicine Hannover, Hannover, Germany.
Unit for Research Data Management, University of Veterinary Medicine Hannover, Hannover, Germany.
Jung, Klaus
Institute of Animal Genomics, University of Veterinary Medicine Hannover, Hannover, Germany.
Geburek, Florian
Clinic for Horses, University of Veterinary Medicine Hannover, Hannover, Germany.
Grant Funding
ContiTech Deutschland GmbH
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