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Topic:Animal Science
Animal Science and horses encompass the study of equine biology, physiology, and management practices aimed at understanding and improving horse health, welfare, and performance. This field integrates various scientific disciplines, including genetics, nutrition, reproduction, and behavior, to address the needs of horses in diverse contexts such as sports, work, and companionship. Research in this area often focuses on optimizing feeding strategies, enhancing breeding programs, and developing effective health management protocols. Additionally, studies explore the genetic factors influencing traits such as athleticism and disease resistance, as well as the impact of environmental and management conditions on horse behavior and welfare. This page gathers peer-reviewed research and scholarly articles that investigate the scientific principles underpinning equine science and their practical applications in horse care and management.
Deep freezing of horse embryos. Fourteen horse embryos recovered non-surgically on Days 6-8 after ovulation (Day 0) were cooled slowly to - 35 degrees C (7 embryos) or - 40 degrees C (7 embryos) and stored in liquid nitrogen (- 196 degrees C) for 4-98 days. Surgical transfer of the thawed embryos to unmated recipient mares that had ovulated - 2 to + 1 days with respect to the embryo donors resulted initially in the establishment of 4 conceptuses. However, only one mare maintained her pregnancy to term.
Equine endometrium at pre-implantation stages of pregnancy has specific glycosylated regions. Pre- and post-implantation endometrial tissue was removed from equids at known stages of pregnancy. Thin sections of this material were reacted with fluorescein conjugates of several lectins. Two lectins in particular, with specificities for L-fucose and N-acetylglucosamine, reacted strongly with the endometrial epithelium. The reaction for N-acetylglucosamine occurred in small patches on the endometrial epithelium and extended to cover the entire epithelial surface and endometrial glands after implantation. L-Fucose was observed in larger surface deposits particularly at the openings of endom...
Humoral, hormonal and behavioral correlates of feeding in ponies: the effects of meal frequency. The effect of meal frequency on body fluid, glucose, triiodothyronine (T3), heart rate and behavior was measured in 10 ponies. A simple reversal design was used in which each pony received one meal/day (1X) for 2 wk and six meals/day (6X) for 2 wk. The total intake/day was held constant. Feeding was followed by a rise in plasma levels of glucose, T3, protein and osmolality. One large meal was followed by significantly greater changes in all of the variables than was a meal one-sixth the size. Plasma T3 rose from 41 +/- 5 (SE) ng/liter before feeding to 43 +/- 5 ng/liter following a small meal,...
Investigations of the vertebrate hosts of eastern equine encephalitis during an epizootic in Michigan, 1980. A study was undertaken to investigate an increase in reported cases of clinical encephalitis due to eastern equine encephalitis (EEE) virus in horses and to determine the natural vertebrate hosts of that virus. Horses, birds, and small mammals were sampled at sites in a contiguous area in St. Joseph and Kalamazoo counties, Michigan, from 25 August to 5 September 1980. Serum samples from four horses acutely ill with encephalitis and 16 of 39 pasture mates of ill horses had neutralizing (N) antibody against EEE virus (46.5%); no viruses were isolated from these 43 sera. None of 24 draft horses f...
The effects of caloric dilution on meal patterns and food intake of ponies. In order to determine if horses will increase their intake in response to caloric dilution, four pony geldings were fed ad lib a mixed grain diet either undiluted (3.4 Mcal/kg of digestible energy) or diluted (wt/wt) with 25% sawdust (2.6 Mcal/kg) or with 50% sawdust (1.7 Mcal/kg). The mean daily caloric intake was 17,457 kcal (3.4 Mcal diet), 17,546 kcal (2.6 Mcal diet) and 12,844 kcal (1.7 Mcal). The mean time spent eating was 246 (3.4 Mcal), 351 (2.6 Mcal), and 408 (1.7 Mcal) minutes/day. Meal size increased and meal frequency decreased with increasing dilution. The median long survivorship...
The incidence of Chorioptes bovis (Acarina: Psoroptidae) on the feet of horses, sheep, and goats in the Netherlands. The feet of horses, sheep, and goats of different breeds and from many different localities were examined for Chorioptes bovis. In horses, mites were mainly found in the Belgian and Frisian breeds (40% and 62% infected, respectively). In sheep and goats, respectively 63% and 86% were infected. In horses as well as in sheep and goats, mange-lesions were rarely seen. A number of sheep and goats were examined for mites and lesions quantitatively. In sheep all mites were restricted to the region close to the accessory digits and the claws. In goats the average number of mites was higher than in sh...
Chemical immobilization and blood analysis of feral horses (Equus caballus). Combinations of etorphine hydrochloride and xylazine hydrochloride in different dosages were tested for their efficacy as immobilizing agents on 16 recently captured feral mares in corrals. The results of these trials led to the utilization of a standard combination of 5.5 mg of etorphine hydrochloride, 150 mg of xylazine hydrochloride, and 3 mg of atropine sulfate in a 7-ml dart syringe for field capture. This combination was used, administered by dart gun from helicopters, to capture 87 free-ranging feral horses from about 80 bands. Five mares died at the time of capture and the remains of t...
Enhanced biliary bilirubin excretion after heparin-induced erythrocyte mass depletion. The effect of large-dose heparin therapy on erythrocyte mass depletion in ponies was investigated to determine whether stimulation of reticuloendothelial cell activity and catabolic function would be evidenced by enhanced catabolism of heme to bilirubin. Ponies with chronic external biliary fistula were used to examine biliary excretion of bilirubin both before and after heparin loading (107 U/kg, IV, plus 320 U/kg, subcutaneously) and at maintenance dosages of 320 U/kg given (subcutaneously) at 12 and 24 hours after initial loading with heparin. Results indicated that by 48 hours after ponies...
Effect of upper airway CO2 on breathing in awake ponies. We determined whether the [CO2] in the upper airways (UA) can influence breathing in ponies and whether UA [CO2] contributes to the attenuation of a thermal tachypnea during periods of elevated inspired CO2. Six ponies were studied 1 mo after chronic tracheostomies were created. For one protocol the ponies were breathing room air through a cuffed endotracheal tube. Another smaller tube was placed in the tracheostomy and directed up the airway. By use of this tube, a pump, and prepared gas mixtures, UA [CO2] was altered without affecting alveolar or arterial PCO2. When the ponies were at a neut...
Horse kidney neutral alpha-D-glucosidase: purification of the detergent-solubilized enzyme; comparison with the proteinase-solubilized forms. Neutral alpha-D-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20) from horse kidney brush-border membranes was solubilized using Emulphogene BC 720 and purified by an affinity chromatography technique. The enzyme preparation (390-fold purified), which was free of other known microvillus hydrolases, exhibited one precipitate line in crossed immunoelectrophoresis and migrated as a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. Several criteria (charge-shift crossed immunoelectrophoresis and hydrophobic chromatography) revealed the purified detergent form of t...
Complexities in the denaturation of horse metmyoglobin by guanidine hydrochloride. The denaturation of horse metmyoglobin by guanidine hydrochloride was studied at pH 6.4 and 25 degrees C. Measurements of both the peptide circular dichroism and the absorbance in the Soret region suggest that the extent of renaturation strongly depends on the time interval during which the protein is exposed to concentrated solutions of the denaturant. From the equilibrium measurements of the absorption in the Soret region, it is concluded that the unfolding of metmyoglobin is complex. This is further supported by kinetic studies of denaturation which suggest the occurrence of the least four ...
Serum antibodies to Leptospira bratislava in Swedish pigs and horses. Sera from 116 and 89 Swedish pigs and horses respectively were examined for the presence of antibodies to L. bratislava. Antibodies were found in 18.1 and 49.4% respectively of pigs and horses examined. Presence of serum antibodies was not associated with clinical signs of infection.
Equine whole saliva: variability of some major constituents. Whole saliva was collected from six horses over a period of five weeks in sufficient volume for the analysis of 10 constituents. There was considerable variation in the concentration of the analytes both between horses and between different days in the same horse. The most variable constituent was sodium, and the least variable was glucose, but this was derived from the sweet used to stimulate salivation. The use of whole saliva as a fluid for investigation would depend on achieving the minimum variability possible.
Isolation of equine coital exanthema virus (equine herpesvirus 3) from the nostril of a foal. The virus causing equine coital exanthema (equine herpesvirus 3) was isolated from a lesion on the nostril of a 2-month-old foal. One week after the mare had returned from a stallion station, vesicular lesions developed on her vulva. They were diagnosed clinically as coital exanthema, and 5 days later a lesion developed on the nostril of her foal. This case is an example of horse-to-horse transmission of coital exanthema virus without coitus. A laboratory diagnosis is necessary to differentiate viruses that cause vesicular lesions about the oral and nasal cavities of horses.
Inherited nuclear cataracts in the Morgan horse. Congenital cataracts affecting the fetal and embryonal lens nucleus were found in 12 Morgan horses. Ten of the 12 affected animals were sired by the same stallion and the condition also affected his female half sibling. Although females were almost three times more likely to be affected than males (9 vs 3), the difference was not significant. The ratio of 11 normal to 10 affected offspring by the affected stallion is compatible with an autosomal dominant mode of inheritance.
Effects of level of dietary protein and exercise on growth rates of horses. Rates of growth were measured in two-year-old (Experiment 1) and nine-month-old (Experiment 2) horses fed high (12 or 14 per cent) or low (6 or 8 per cent) crude protein diets with one of two levels of exercise (0 or 12 km trotting per day at 12 km/h). In the non-exercised horses feed intakes and growth rates were greater on the high than on the low protein diets. Exercise increased feed intakes and growth rates of horses on the low but not the high protein diets, so that in the exercised groups there were no significant differences in feed intakes or growth rates between the horses on the two...
The isoelectric focusing of keratins in hair followed by silver staining. An isoelectric focusing method followed by silver staining has been developed for the study of keratins which is as effective as two-dimensional electrophoresis and fluorography for hair species identification. Hair from dogs, rabbits, horses, cows, guinea-pigs, donkeys, sheep and cats were successfully identified. Narrow pH ranges were used to observe heterogeneity in human hair. Although this heterogeneity may be affected by environmental conditions, it may be of use in criminalistics.
Equine blood serum calcium and phosphorus concentrations in progressive nutritional hyperparathyroidism. Weekly blood serum samples from 20 yearling horses, divided into equal groups, receiving diets with total calcium to inorganic phosphorus ratios of 1,5/1 and 0,2/1, were analysed for calcium and phosphorus concentrations. This was done over a continuous 23 week period, divided into 2 sub-periods of 16 and 7 weeks each. During the first period complete diets were provided ad lib but were restricted during the second period to accentuate any effects of calcium deficiency and phosphorus excess. Falling calcium levels and rising phosphorus levels during period 1 and a reverse trend during period 2...
Effects of exercise and level of dietary protein on digestive function in horses. Rates of passage of fluid and particulate digesta markers and apparent digestibility estimated by three methods were compared in yearling horses fed high (14 per cent) or low (8 per cent) crude protein diets with one of two levels of exercise (0 or 12 km trotting per day at 12 km/h). Mean retention times (MRT) of the fluid marker (51Cr-EDTA) were shorter than those of the particulate marker (ruthenium-phenanthroline). There were no significant effects of dietary protein level on passage of either marker or on apparent digestibility of dry matter. Exercise increased voluntary feed intake and ap...
Arbovirus investigations in Argentina, 1977-1980. IV. Serologic surveys and sentinel equine program. Serologic surveys of wild and domestic birds, wild mammals, and horses were conducted during arbovirus field studies in Argentina from 1977 through 1980, a non-epizootic interval. The prevalence of neutralizing antibodies to eastern equine encephalitis (EEE) was consistently higher than to western equine encephalitis (WEE) virus in all species and all areas. The presence of antibodies in short-lived avian species and in young unvaccinated horses and the demonstration of seroconversions in horses during the period, indicated that these viruses are either enzootic in, or annually reintroduced in...
In vivo bone strain in the equine tibia before and after transection of the peroneus tertius muscle. The present study was undertaken to determine the influence of the peroneus tertius muscle on the loading regime of the tibia in the horse. Strain gauge rosettes were bonded to the cranial and caudal cortices of the left and right tibiae in six Shetland ponies. In vivo bone strain recordings were made before and after unilateral transection of the peroneus tertius muscle. Relatively large individual variations in response to transection were observed in both the experimental and the contralateral control limbs. The principal strain values on the cranial and caudal cortices increased during the...
Arbovirus investigations in Argentina, 1977-1980. I. Historical aspects and description of study sites. This is the introductory paper to a series on the ecology of arboviruses in Argentina. Epizootics of equine encephalitis have occurred since at least 1908, principally in the Pampa and Espinal biogeographic zones, with significant economic losses; human cases of encephalitis have been rare or absent. Both western equine and eastern equine encephalitis viruses have been isolated from horses during these epizootics, but the mosquitoes responsible for transmission have not been identified. A number of isolations of Venezuelan equine encephalitis (VEE) virus were reported between 1936 and 1958 in ...
[An undesirable drug interaction in horses? Complications which can occur during the administration of coumarin derivatives and phenylbutazone]. A study of the literature was done because of questions asked in a court of justice concerning possible poisoning in a jumper, resulting from administration of both phenylbutazone and a coumarin derivative within a particular period. In view of the mechanisms of action and the pharmacokinetic characteristics of the agents, these forms of combined treatment are also highly inadvisable in horses.
Kinetic studies of the unfolding-refolding of horse muscle phosphoglycerate kinase induced by guanidine hydrochloride. The kinetics of the unfolding and refolding of horse muscle phosphoglycerate kinase were studied with three different signals: fluorescence emission intensity at 336 nm (excitation at 292 nm), ellipticity at 220 nm, and enzyme activity. The results corroborate the conclusion on the existence of intermediates in the folding pathway obtained from equilibrium studies. Kinetic studies showed at least two phases of refolding, as revealed by fluorescence as well as by circular dichroism measurements. During the fast phase, an intermediate was formed with a fluorescence intensity higher than that of ...
