Analyze Diet

Topic:Animal Science

Animal Science and horses encompass the study of equine biology, physiology, and management practices aimed at understanding and improving horse health, welfare, and performance. This field integrates various scientific disciplines, including genetics, nutrition, reproduction, and behavior, to address the needs of horses in diverse contexts such as sports, work, and companionship. Research in this area often focuses on optimizing feeding strategies, enhancing breeding programs, and developing effective health management protocols. Additionally, studies explore the genetic factors influencing traits such as athleticism and disease resistance, as well as the impact of environmental and management conditions on horse behavior and welfare. This page gathers peer-reviewed research and scholarly articles that investigate the scientific principles underpinning equine science and their practical applications in horse care and management.
Veterinarian and breeder perspectives surrounding ovum pick-up and intracytoplasmic sperm injection in mares: A survey study.
Equine veterinary journal    August 28, 2026   doi: 10.1002/evj.70323
Lewis N, Spanner J, Daels P, Arango JC, Morganti M, Dini P, Allen K, Mullan S.There is an ethical debate about the application of ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) in mares. Objective: To survey the opinions of equine veterinarians and breeders of sport horses on the ethics of using OPU-ICSI in mares. Methods: Cross-sectional descriptive study. Methods: Three surveys were distributed. The first targeted breeders ('Breeder Survey') who were actively involved in sport horse breeding. The second targeted veterinarians performing OPU ('OPU-Reprovets Survey'). The third targeted general equine practitioners ('Practitioner Survey'). There were six...
Comparative genomics of Standardbred horses reveals candidate regions under selection for harness racing performance.
Journal of equine veterinary science    August 25, 2026   106133 doi: 10.1016/j.jevs.2026.106133
Harness racing performance in horses is a complex polygenic trait influenced by genetic background, breeding history, training, and environment. Understanding genomic patterns shaped by long-term artificial selection provides insights into performance-related traits and breed-specific genomic variation. Objective: This study aimed to characterize genomic differentiation, population structure, and candidate genomic regions potentially influenced by historical selection in Standardbred horses using comparative whole-genome sequencing involving diverse horse breeds. Methods: Whole-genome sequenci...
Relationship Between Feed Olfactory Exploration and Consumption in Horses.
Animals : an open access journal from MDPI    August 20, 2026   Volume 16, Issue 16 2599 doi: 10.3390/ani16162599
Wnuk E, Janicka W, Łuszczyński J, Wojtyniak A, Jagusiak W, Stachurska A.The aim of the study was to evaluate the relationship between horses' behaviour during olfactory exploration and following consumption of the same food containing herbs, either previously exposed to the herb odour or not. Twenty horses' responses to 14 herbs were compared in a smelling test, when the horses sniffed a herb, and after 20 min a consumption test, when they consumed oats mixed with the same herb. Before the tests, the horses were exposed to the scent of seven herbs, whereas the remaining seven herbs were non-pre-exposed. The results show that horses consumed oats with herbal additi...
Measuring Activity-Rest Condition by Using Two Different Accelerometer Devices in Trained and Retired Horses.
Animals : an open access journal from MDPI    August 18, 2026   Volume 16, Issue 16 2570 doi: 10.3390/ani16162570
Giannetto C, Aragona F, Fazio F, Giudice E, Zumbo A, Piccione G.To advance technological applications in animal welfare assessment, this study evaluated resting activity as a component of total locomotor activity (TLA) in horses using two different accelerometer devices: a collar-mounted device containing a single-axis accelerometer that records physical movement, and a three axes accelerometer that classifies the measured physical activity into nine different activities. Ten clinically healthy Italian saddle horses were enrolled in the study and divided into two groups on the basis of their lifestyle. Group A consisted of regularly trained horses housed c...
Validation of a handheld smartphone markerless method for quantifying thoracolumbar flexion-extension range of motion in horses at trot.
Equine veterinary journal    August 14, 2026   doi: 10.1002/evj.70310
Christensen MR, Kirkegaard J, Berg K, Andresen KR, Skov Hansen S, Key K.Back pain and poor performance in horses are difficult to assess objectively and thoracolumbar flexion-extension range of motion (FE) is not reliably evaluated by subjective observation alone. A handheld, smartphone-based markerless computer vision system (RealHorse®; RH) may enable objective clinical and field-based FE assessment but requires validation against an established optical motion capture reference system (Qualisys®; QS). Objective: To compare the accuracy and precision of RH relative to QS for measuring FE in horses trotting on a straight line and on a circle. Methods: Cross-sect...
Questionnaire-Based Investigation into Stallion Management Practices in 28 Countries.
Journal of equine veterinary science    August 14, 2026   106128 doi: 10.1016/j.jevs.2026.106128
de Oliveira RA, Aurich J, Aurich C.Modern stud farm practices may limit stallion social contact, with potential welfare and safety implications. Objective: To describe housing, management and safety outcomes for stallions kept at horse breeding operations throughout the world and to assess regional differences. Methods: An online questionnaire was distributed via mailing lists and WhatsApp; 116 complete responses from breeding operations were collected and analysed. The focus was on management of the breeding facility, and stallion welfare indicated by management, handling and housing. Results: Respondents were from Europe (40....
Extreme male reproductive skew limits effective population size in American Standardbred horses.
The Journal of heredity    August 13, 2026   esag062 doi: 10.1093/jhered/esag062
Waples RS, Khanshour AM, Cothran EG.Standardbred horses are widely used for harness racing in North America, Europe, Australia, and New Zealand. In the U.S., two gaits (Pacers and Trotters) are genetically differentiated at a level typical for different horse breeds. We used the complete pedigree for all registered American Standardbred foals born 1970-2014 (>200 K Trotters and > 500 K Pacers) to calculate annual and lifetime reproductive skew and its consequences for the effective number of breeders per year (Nb) and effective size per generation (Ne). With rare exceptions, females can only produce 0 or 1 foal per year, w...
Characterization of Lipizzan Horse Population From Lipica Stud Using Molecular and Pedigree Data.
Animal genetics    August 12, 2026   Volume 57, Issue 4 e70182 doi: 10.1002/age.70182
Ferme T, Zorc M, Cotman M, Mesarič M, Dovč P.In this study, we analysed genetic diversity, population structure and inbreeding in the Lipica Stud Farm population by integrating pedigree records, microsatellite (STR) and genome-wide SNP data generated using the GGP Equine 70K BeadChip. The dataset comprised 233 horses from the Lipica Stud Farm, which served as the reference population for comparisons of diversity estimates based on pedigree-, STR- and SNP data. The STR data were originally generated for routine parentage verification and were included here to compare inbreeding estimates based on pedigree, STR and genome-wide SNP data. Pe...
Choice for the horse is paramount: Owners’ experiences in facilitating friends, forage, and freedom in their management practices.
Animal welfare (South Mimms, England)    August 10, 2026   Volume 35 e48 doi: 10.1017/awf.2026.10107
Watson WL, Jessiman LJ, Dwyer C.Owned horses rely on people for their behavioural needs, such as access to conspecifics, constant access to low nutrient density, fibrous forage and freedom of movement; otherwise known as friends, forage and freedom (3Fs). Restricting the 3Fs prevents horses from achieving optimal welfare. We carried out 14 semi-structured interviews in the UK and Ireland to examine horse owners' experiences, attitudes and beliefs around the provision of the 3Fs. Most participants were female, aged from 25 to 65 years, resident in the UK and kept their horse at a livery facility. A reflexive thematic analysis...
Genome-Wide Identification and Characterization of the Wnt Gene Family in Donkey (Equus asinus): Phylogenetic Analysis and Expression Profiling.
Biology    August 6, 2026   Volume 15, Issue 15 1323 doi: 10.3390/biology15151323
Zhu Q, Jing Y, Zheng Y, Khan MZ, Liu A, Peng Y, Wang C.The gene family encodes highly conserved signaling molecules involved in vertebrate development, tissue homeostasis, and cell fate regulation. However, the evolutionary characteristics and potential biological functions of genes in the domestic donkey () remain poorly understood. In this study, we performed the first genome-wide identification and comprehensive characterization of the donkey gene family. A total of 19 genes were identified, consistent with the conserved repertoire reported in other mammalian species. Phylogenetic and synteny analyses revealed strong evolutionary conservat...
Phased T2T horse and donkey assemblies from a mule reveal peculiar equid centromere evolution.
Cell genomics    August 6, 2026   Volume 6, Issue 8 101307 doi: 10.1016/j.xgen.2026.101307
We present telomere-to-telomere genome assemblies of a Thoroughbred horse and a donkey derived from their mule offspring. Now adopted and annotated by NCBI as reference genomes, these assemblies resolve previously inaccessible regions, including satellite arrays, duplications, and telomeres. Equids are known to exhibit an uncoupling between satellite DNA and centromeric function. The completeness of these assemblies enabled annotation of both satellite-based and satellite-free centromeres, as well as non-centromeric satellite loci, revealing notable centromeric plasticity. They also allowed de...
Four Haylages, One Metabonome: Defining Diet Driven Metabolic Shifts in Ponies.
Journal of animal physiology and animal nutrition    August 6, 2026   doi: 10.1111/jpn.70099
Moore-Colyer M, Harris P, Daniels S.Haylage is widely fed to all categories of stabled horses across Europe and New Zealand. Haylage can vary from high energy nutrient dense forage to low energy highly fibrous fodder, yet little is known about the impact different haylages have on metabolism in horses. This study provides the first direct comparison of feeding four different haylages: early cut (Lolium multiflorum), later cut (Lolium perenne) rye grass (R1 and R2); early and late cut meadow grass (M1 and M2), on urinary and faecal metabonomics, in ponies at maintenance. Using replicated Latin Square design, eight individually ho...
Implications of dominance deviations for reproductive and morphological traits in the genetic management of the Pura Raza Española horse.
Genetics, selection, evolution : GSE    August 5, 2026   Volume 58, Issue 1 59 doi: 10.1186/s12711-026-01075-4
Perdomo-González DI, Valera M, Molina A, Azor P, Sánchez JP.Dominance is a non-additive genetic effect involving allele interaction at the same locus. In animal breeding, it has often been ignored due to its complexity and the low accuracy of pedigree-based estimates. This study analysed dominance variance components for reproductive traits [age at first foaling (AFF), reproductive efficiency (RE), interval between first and second foaling (I12)] and morphological traits [scapulo-ischial length (SIL), dorsal sternal diameter (DSD), knee circumference (KC)] in Pura Raza Española (PRE) mares. The aim was to assess the magnitude of dominance effects acro...
Advanced 3D Horse Reconstruction: Integrating Image-to-3D Modelling and Non-Rigid Registration for On-Barn Body Measurements.
Animals : an open access journal from MDPI    August 3, 2026   Volume 16, Issue 15 2397 doi: 10.3390/ani16152397
Yuan X, Wang S, Zhu Q, Wang Y, Ruchay A, Pezzuolo A, Wang Q, Li J, Guo H.Precision livestock farming (PLF) relies on high-precision three-dimensional (3D) horse reconstruction and automatic body measurement to support refined breeding management and health surveillance. However, data collection is restricted by building environment noise and hardware layout constraints; complex equine body shapes and large individual variations induce local geometric distortions in reconstructed models, limiting field deployment. Drawing on generative 3D reconstruction, this study develops the first image-to-3D pipeline that leverages three consumer depth cameras to reconstruct hig...
Effect of neonatal rib fracture repair on racing and sales performance in Thoroughbreds.
Equine veterinary journal    July 28, 2026   doi: 10.1002/evj.70218
Young LM, Courtney ER, Freeland RB.The racing outcome of horses that have undergone surgical repair of neonatal rib fractures is not well documented. Objective: To evaluate the impact of surgical repair of neonatal rib fractures on subsequent sales and racing performance in Thoroughbreds. Methods: Retrospective cohort study. Methods: Medical records of neonatal Thoroughbreds that underwent surgical repair of rib fractures at a referral hospital between 2014 and 2025 were reviewed. Sales and racing data were obtained from publicly available databases. Outcomes assessed included sale price at weanling and yearling sales, total ca...
Differences between horses (Equus caballus) displaying oral and locomotor stereotypies in their responses to a stable mirror.
Behavioural processes    July 28, 2026   105415 doi: 10.1016/j.beproc.2026.105415
Lewis K, McBride SD, Parker MO, Faithfull R, Proops L.Recent cross-species analyses suggest that the different forms of stereotypic behaviour have heterogeneous causal mechanisms. In ungulates, there are indications that some forms of locomotor stereotypy may be linked to social factors. Using the domestic horse as a model species within this clade, we investigated whether individuals displaying the locomotor stereotypy box-walking (n=6) demonstrated greater responses to a stable mirror, intended to mimic presence of a conspecific, than horses displaying the oral stereotypy crib-biting (n=5) and non-stereotypic horses (n=5). After a 24hr baseline...
Somatic cell nuclear transfer as a complementary tool for genetic conservation of the Peruvian Paso horse.
The Journal of reproduction and development    July 28, 2026   doi: 10.1262/jrd.2026-068
Cortez JV, Segura Portocarrero GT, Murga Valderrama NL, Bardales Escalante W, Encina Ruiz R, Velásquez Castillo CL, Grupen CG, Maicelo Quintana JL.The Peruvian Paso horse, a breed of national significance in Peru known for its unique gait, has been selectively bred for nearly 500 years and now faces potential risks due to reduced genetic diversity. To investigate the potential of somatic cell nuclear transfer to enhance genetic conservation strategies, a program was initiated to produce cloned Peruvian Paso foals. Skin biopsies from a highly awarded stallion with exceptional breed-defining traits were used as the source of donor somatic cells. Of the 15 cloned embryos reconstructed, seven blastocysts were produced, and five were transfer...
Species-Specific Mechanisms of Sperm Transport: Predominant Roles of Motility in Ewes and Uterine Contractility in Mares.
Reproduction (Cambridge, England)    July 28, 2026   xaag068 doi: 10.1093/reprod/xaag068
Barakat E, Caldas E, Reigner F, Barrière P, Blard T, Lasserre O, Cognié J, Tsikis G, de Graaf SP, Druart X.The factors controlling sperm transport within the female genital tract vary among species. The aim of this study was to quantify the respective roles of sperm motility and uterine contractions in passage of sperm within the reproductive tract of the mare and ewe. Sperm imaging was performed in vivo within the female genital tract of mares and ewes using probe-based confocal laser endomicroscopy (pCLE) after intrauterine insemination during two stages of the cycle characterized by high (oestrus) or low (luteal phase) uterine contractility. Fluorescent microbeads (2 µm in diameter) were insemi...
Combined effects of pre-freeze cholesterol-loaded cyclodextrin and post-thaw pentoxifylline on the functional longevity of cryopreserved stallion sperm.
Journal of equine veterinary science    July 28, 2026   Volume 165 106100 doi: 10.1016/j.jevs.2026.106100
Vilela VMT, Zutter BM, Miranda BN, Baldini PHO, Gomes LLO, Silva DF, Diniz JHW, Sancler-Silva YFR, Ignácio FS, Papa FO, Monteiro GA.Cryopreservation causes structural and metabolic damage to stallion spermatozoa, impairing post-thaw functional competence. Objective: This study evaluated whether cholesterol-loaded cyclodextrin (CLC) supplementation before freezing, combined with pentoxifylline (PTX) after thawing, improves the functional quality, oxidative status, mitochondrial activity, and preliminary fertility outcomes of cryopreserved stallion semen. Methods: Semen from twelve stallions was assigned to four CLC concentrations (0, 1, 1.5, and 2 mg), frozen, thawed, and divided into subgroups with or without PTX supplemen...
A Global Structural Variation Map Reveals Novel Variants for Body Conformation and Racing Ability in Horses.
Genomics, proteomics & bioinformatics    July 27, 2026   qzag071 doi: 10.1093/gpbjnl/qzag071
Gong Y, Liu X, Zheng Z, Zeng S, Zhang Y, Pu Y, Pan J, Ma Y, Jiang L.The selection of horses has been instrumental in shaping long-range mobility, advancing warfare strategies, and diversifying equine phenotypes. However, the structural variants (SVs) favored by this process remain largely unexplored. Utilizing high-fidelity Pacific Biosciences sequencing, we assembled three high-quality horse genomes, incorporating 237 Mb (9.48%) of novel sequences relative to the existing reference genome. These assemblies achieved an average Benchmarking Universal Single-Copy Orthologs completeness of 95.1%. By employing a graph-based approach, we genotyped 23,163 high-con...
Environmental and genetic effects on measured hock bone proportions in young Polish Warmblood horses undergoing performance test training for breeding horses.
Veterinary journal (London, England : 1997)    July 25, 2026   106795 doi: 10.1016/j.tvjl.2026.106795
Buczkowska R, Hecold M, Verwilghen D, Lewczuk D.The hock bone proportions in young Polish Warmblood horses (69) were studied based on x-ray image measurements done during official breeding horse performance testing. Six measurements were used to identify 4 width and length proportion indices. The radiological examinations were done before and after horse training, which consists of work with or without the rider at walk, trot and canter (40-50min) with some light class (< 100cm) jumping (<12 jumps) 6 days a week. The training effect was investigated using analysis of variance (procedure MIXED, Statistical Analysis System). The statist...
Three-year evaluation of anthelmintic treatment efficacy against equine strongyles in France using FECRT.
International journal for parasitology. Drugs and drug resistance    July 24, 2026   Volume 31 100663 doi: 10.1016/j.ijpddr.2026.100663
Reduced anthelmintic efficacy against strongyles in equids is a widespread concern, but up-to-date field data are scarce. The aim of this study was to evaluate, over a three-year period, the efficacy of the anthelmintic (AH) molecules available in France (fenbendazole, pyrantel, ivermectin and moxidectin) against strongyles in equids using faecal egg count reduction tests (FECRTs). A total of 104 complete FECRTs (i.e., required sample size reached) were conducted in 98 groups, involving 749 equids kept in 70 facilities. Fenbendazole-resistant populations were detected in 8/9 groups (89%) from ...
Phenotypic Definition Influences Genetic Inference and Predictive Ability for Reining Performance in Quarter Horses Using Random Regression Models.
Animal science journal = Nihon chikusan Gakkaiho    July 23, 2026   Volume 97, Issue 1 e70223 doi: 10.1111/asj.70223
Santana ML, Bignardi AB.Reining performance is influenced by subjectivity and competition structure, challenging genetic evaluation. This study estimated genetic parameters for reining performance in Brazilian Quarter Horses using random regression models (RRM) and evaluated how alternative phenotypic definitions affect genetic inference and predictive ability. A dataset comprising 21,165 longitudinal records from 1603 horses was analyzed, comparing within-group rank (RANK) and Blom-transformed rank (BLOM). Model comparison indicated that cubic RRM provided the best fit for both phenotypes. Heritability estimates wer...
Growth Performance, Milk Productivity, Productive Longevity, and Milk Composition of Mugalzhar Horses.
Animals : an open access journal from MDPI    July 22, 2026   Volume 16, Issue 14 2272 doi: 10.3390/ani16142272
Toishimanov M, Alikhanov O, Aubakirov K, Karibayeva D, Kargaeva M, Sapakhova Z, Begaliyeva D, Kanat R, Tugunova Y, Daurov D, Daurova A, Zhambakin K....The Mugalzhar horse is an important native breed of Kazakhstan, valued for its adaptability, milk production, and meat productivity under extensive pasture-based management. This study evaluated the relationships among first-lactation milk yield, productive longevity, lifetime milk production, growth, morphometric traits, and milk composition in the Mugalzhar horse breed using data from 100 mares with complete lifetime production records, 108 adult mares and 12 stallions for morphometric evaluation, and 68 foals (35 colts and 33 fillies) for growth analysis. The study was conducted on Mugalzha...
Correction: Review: Genomic insights into the adaptive traits and stress resistance in modern horses.
Stress biology    July 20, 2026   Volume 6, Issue 1 50 doi: 10.1007/s44154-026-00327-z
Jafari H, Abebe BK, Cong L, Ahmed Z, Zhaofei W, Sun M, Muhatai G, Chuzhao L, Dang R.No abstract available
Phenotypic and genetic factors that affect the likelihood of Standardbred foals achieving a race start.
Scientific reports    July 20, 2026   doi: 10.1038/s41598-026-63159-x
Tanner JC, Beatson PR, Hickford JGH, Barrell GK.This study was conducted to quantify the probability of a Standardbred foal attaining early career milestones and to estimate some environmental and genetic factors that influence achievement of those milestones. Data for all Standardbred foals registered at birth in New Zealand from 1995 to 2018 were used to estimate variance components and fixed effects influencing attainment of three early-career traits; registered with trainer (REG), starting in a trial (TRIAL) and starting in a race (RACE). The study population comprised 65,256 foals. Of these, 65.5% attained REG, 55.4% progressed to TRIA...
Global distribution of cattle, horses, goats, sheep and buffaloes at 1 km resolution for 2000-2022 based on subnational census data and spatiotemporal machine learning.
PeerJ    July 17, 2026   Volume 14 e21494 doi: 10.7717/peerj.21494
Parente L, Ehrmann S, Hengl T, Fritz S, Bonannella C, Malek Ž, Gonzalez Fischer C, Perez K, Stanimirova R, Meyer C, Wisser D, Cinardi G, Sloat L.The article describes the production and evaluation of annual livestock densities and headcounts of cattle, horses, sheep, goats and buffaloes (including 95% probability prediction intervals) at 1 km spatial resolution for the 2000-2022 period using spatiotemporal machine learning. A compilation of subnational livestock census data has been imported, harmonized and used as reference data (55,336 census polygons and 939,257 individual data entries; covering 147 countries) to build predictive models. A large stack of multi-source harmonized raster data sets (128 individual layers) were used as f...
Demographic change and selection patterns in thoroughbred racing horses over the 20th century.
iScience    July 17, 2026   Volume 29, Issue 8 116830 doi: 10.1016/j.isci.2026.116830
Asadollahpour Nanaei H, Seguin-Orlando A, Negara K, MacLeod JN, Kalbfleish T, Orlando L.The Thoroughbred horse is a legacy of centuries of selective breeding for speed, emerging from the admixture of British mares and imported Oriental stallions. While previous work explored its genetic diversity, inbreeding, and selection signatures, the breed demographic and selection dynamics during the 20-century remain poorly understood. Here, we sequenced the genome of Phar Lap, a late-1920s/early-1930s racing champion, and analyzed the largest time-series of Thoroughbred whole-genome sequences, within a global panel of 850 horse genomes. Demographic modeling reveals a marked decline in gen...
Preliminary Metabolomic Analysis: Serum Metabolomic Dynamics During Estrus Synchronization in Kazakh Mares.
Animals : an open access journal from MDPI    July 17, 2026   Volume 16, Issue 14 2222 doi: 10.3390/ani16142222
Liu J, Gan J, Yao X, Wang J, Ren W, Meng J, Zeng Y.This study aimed to analyze the differences in serum metabolomes at different stages of estrus synchronization in Kazakh horses, to characterize dynamic changes in serum metabolites during estrus synchronization, and to screen potential metabolic markers and key regulatory pathways. Four sampling stages (M1: intravaginal device insertion; M3: device removal, labeled consistently with the pre-experiment timeline; P: 24 h post-PG injection; L: tertiary follicle emergence) were selected. The research results showed that 37 significantly different metabolites (DAMs) were identified in the M1-vs.-M...
Sequencing and Differential Analysis of miRNA in the Ovaries of Kazakh Horses During Estrus and Anestrus States.
Animals : an open access journal from MDPI    July 15, 2026   Volume 16, Issue 14 2197 doi: 10.3390/ani16142197
Liu J, Yao X, Meng J, Wang J, Zeng Y, Ren W.To reveal the regulatory role of miRNA in the estrous cycle transition of Kazakh horse ovaries and improve their reproductive efficiency, this study took the ovaries of Kazakh horses in the estrus period (DY group) and the quiescent period (DB group) as research objects. Small RNA sequencing and bioinformatics analysis were used to systematically analyze the expression characteristics and functional pathways of miRNA under the two physiological states. A total of 161 million high-quality miRNA tags were obtained through sequencing. Principal component analysis showed that the intra-group repea...
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