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Topic:Comparative Study
Comparative studies in equine research involve the systematic analysis of different horse breeds, management practices, or physiological responses to identify variations and similarities. These studies are instrumental in understanding how different factors influence health, performance, and behavior in horses. Common areas of comparison include genetic traits, nutritional requirements, disease resistance, and response to training. By evaluating these differences, researchers can develop targeted strategies for breeding, healthcare, and training. This page aggregates peer-reviewed research studies and scholarly articles that focus on the methodologies, findings, and implications of comparative studies in the context of equine science.
Further observations on the morphology and sensitive innervation of the retractor penis muscle in several species of ungulates. The retractor penis muscle originates from the vertebrae in pigs, horses, cattle and goats; it arises from the rectum in sheep. In all the species considered, sensitive innervation was found. This was represented by encapsulated receptors. Nervous vegetative supply, made up of isolated or assembled ganglion cells, was also present. Among the numerous sensory nerve endings found in pigs, goats and sheep were Pacinian, Pacinian-like, Golgi-Mazzoni corpuscles and Krause's end bulbs (genital corpuscles). Few Pacinian and Pacinian-like corpuscles were found in cattle and horses. A hypothesis on the...
Pinealitis accompanying equine recurrent uveitis. There is no direct verification of pineal gland involvement in human uveitis. Specimens of pineal tissue are not available during active uveitis in human patients. Naturally occurring uveitis in horses gives us an opportunity to examine tissues during active ocular inflammation. We examined the pineal gland of a horse that was killed because it had become blind during an episode of uveitis. The clinical history and histopathology of the eyes were consistent with post-leptospiral equine recurrent uveitis. The pineal gland of this horse had significant inflammatory infiltration consisting mainly...
Intraosseous pressure and pathologic changes in horses with navicular disease. Navicular bone intraosseous pressure, gross pathologic, histologic, and histochemical data were collected from 8 horses with navicular disease and 4 control horses. Simultaneous navicular bone intraosseous, medial palmar arterial, and saphenous venous pressures were measured for the left and right forelimbs of each horse under general anesthesia. Gross pathologic evaluation included grading of changes on the flexor surface of the navicular bone. Safranin-O-fast green-stained sections were used for histologic-histochemical grading of the hyaline articular and fibrocartilage surfaces of the navi...
A comparison of the traditional Chinese versus transpositional zangfu organ association acupoint locations in the horse. The traditional Chinese (TC) and transpositional (TP) methods of animal acupoint location result in different acupoint charts. Representative TC and TP equine zangfu organ shu-association acupoint charts are compared to each other and to a human chart. Despite their differences, practitioners of both methods appear to achieve equally effective therapeutic results--a phenomenon termed "traditional Chinese/transpositional equal efficacy" (TTEE). Common veterinary acupuncture practices, traditional Chinese medical theory, spinal cord anatomy, and a preliminary equine "association segment" chart a...
Quantification of hind limb lameness in the horse. The three-dimensional optoelectronic locomotion analysis system SELSPOT II was used for kinematic studies of hind limb locomotion patterns. Two groups, 11 sound horses and 15 horses suffering from hind limb lameness, were examined at the trot. Both graphical and quantitative analyses were compared in sound and lame horses. The parameter hip acceleration quotient (HAQ), using the different peaks of vertical acceleration of one hip during one stride, proved to be a suitable value for quantitative analysis of hind limb lameness. In sound horses the HAQ ranged from 1.03 to 1.54, lame horses showed...
Complexoproductive and antiheparin properties of low density lipoproteins (LDL). VI. Antiheparin activity in blood plasma of different species of vertebrates. Antiheparin activity of plasma of different species of vertebrates depends to a large extent on contents of low density lipoproteins (LDL). High antiheparin activity of the blood plasma of chicken and human corresponds to high contents of LDL and low antiheparin activity of the blood plasma of horse, cow, sheep, dog and pig corresponds to decreased contents of these proteins. Differences in the contents of fibrinogen, acid alfa1-glycoproteins, globulins, alkaline proteins and antithrombin III activity have smaller influence on antiheparin activity in the blood plasma of the examined animals.
Concept of a force-measuring horseshoe. To further develop objective methods in the field of gait analysis a force-measuring horseshoe (FM shoe) has been developed. The ground reaction forces were determined by measuring the degree of displacement between the two solid parts of the shoe. This was done by three removable measuring units (MUs) that were equipped with strain gauges and placed in the toe and in each of the quarter parts. Before performing force recordings the sensors were calibrated with the actual MUs mounted on the shoe. This can be done in the field with a specially developed equipment. The shape of the signal genera...
Species difference in modulation of calcium release by Naja naja kaouthia snake venom cardiotoxin in terminal cisternae from human and equine skeletal muscle. The modulation of Ca2+ release by a cardiotoxin (CTX) from Naja naja kaouthia snake venom was examined in terminal cisternae-containing fractions from equine and human skeletal muscle. Pretreatment with CTX (10 microM) decreased by 27% (human muscle), or had no effect on (equine muscle), the threshold of Ca(2+)-induced Ca2+ release. If terminal cisternae fractions were first preloaded with Ca2+ to greater than 65% of the threshold of Ca(2+)-induced Ca2+ release and then CTX added, an immediate and sustained release of Ca2+ occurred in preparations from both species. Addition of CTX after a Ca2...
Changes in cross-sectional area and capillary supply of the muscle fiber population in equine gluteus medius muscle as a function of sampling depth. The right and left gluteus medius muscles of 4 mature Andalusian stallions were examined by repeated needle biopsy over a specific area and depth, as well as at different depths, to determine whether the cross-sectional area and capillary supply of the various fiber types are homogeneous throughout the muscle. The muscle biopsy specimens were histochemically analyzed for fiber types (myofibrillar adenosine triphosphatase) and capillaries (amylase-periodic acid-Schiff method). Differences between contralateral sites were not identifiable for any of the analyzed variables. Differences between sa...
Insulin-like growth factor binding proteins of equine serum. Ligand blotting analysis of serum from the horse using radiolabelled IGF-I revealed a protein at 96 kDa which was not present in serum from goat, cow, sheep, deer or donkey. These latter species all displayed five labelled bands in the range 24 to 41 kDa. Conversely, these were only weakly labelled in serum from the horse. Size exclusion chromatography of horse serum pre-incubated with radiolabelled IGF-I revealed reduced binding in the 130-kDa peak compared with goat plasma, and ligand blotting analysis indicated the 96-kDa protein was present in this peak. The 96-kDa protein from horse serum...
L chain isotype regulation in horse. I. Characterization of Ig lambda genes. Analysis of 10 cDNA encoding lambda L chains of horse Ig indicated that this species may employ a relatively small number of variable region (V lambda) genes in the splenic B cell population. The V lambda sequences of all of the cDNA analyzed were closely related (> 88% identity at the nucleotide level) and were characterized by a deletion of the amino acid residue at position 3 compared with V lambda sequences so far described in other species. The 10 V lambda sequences could be grouped into three groups, V lambda 1 to V lambda 3, on the basis of a number of linked substitutions. Sequences...
Crystal structure of a complex between electron transfer partners, cytochrome c peroxidase and cytochrome c. The crystal structure of a 1:1 complex between yeast cytochrome c peroxidase and yeast iso-1-cytochrome c was determined at 2.3 A resolution. This structure reveals a possible electron transfer pathway unlike any previously proposed for this extensively studied redox pair. The shortest straight line between the two hemes closely follows the peroxidase backbone chain of residues Ala194, Ala193, Gly192, and finally Trp191, the indole ring of which is perpendicular to, and in van der Waals contact with, the peroxidase heme. The crystal structure at 2.8 A of a complex between yeast cytochrome c pe...
Cross-species comparison of 5-lipoxygenase-activating protein. To identify regions of 5-lipoxygenase-activating protein (FLAP) important for the function of the protein and the binding of leukotriene biosynthesis inhibitors, we performed a cross-species analysis of FLAP. FLAP from all 10 mammalian species analyzed (human, monkey, horse, pig, cow, sheep, rabbit, dog, rat, and mouse) were immunologically cross-reactive and specifically bound leukotriene biosynthesis inhibitors with high affinity. Using the polymerase chain reaction, cDNA clones for FLAP from six species (monkey, horse, pig, sheep, rabbit, and mouse) were isolated and sequenced. The deduced ...
Visual acuity in the horse. We assessed the ease with which horses could learn visual discriminations and measured their resolution acuity. We trained three horses to press their noses against one of two large wooden panels to receive a small food reward. Following training on a series of two-choice discrimination tasks, resolution acuity was measured. Although there was some variability between animals, the best acuity obtained was 23.3 c deg-1. Within the margin of error imposed by limited anatomical data, the obtained values are consistent with predictions based on retinal ganglion cell density estimates and posterior...
A specific stain for the detection of nonheme iron proteins in polyacrylamide gels. Nonheme iron proteins can be visualized as blue bands in native polyacrylamide gels using a staining method that is both simple and rapid. The reaction of potassium ferricyanide with protein-bound iron atoms to form royal blue complexes occurs almost instantaneously and is sensitive enough to detect 1 microgram of analytical-grade ferritin and 2 micrograms of purified ferredoxin from cyanobacteria. No special treatment of reagents or apparatus was necessary. On comparison, this stain was found to be more specific than the Ferene S stain, not detecting bovine serum albumin even when present as ...
Relationship between colloid osmotic pressure and plasma protein concentration in cattle, horses, dogs, and cats. The relationship between colloid osmotic pressure (COP) and protein concentration was investigated for purified proteins and plasma samples obtained from cattle, horses, dogs, and cats. At equivalent concentrations, bovine albumin exerted a COP that exceeded that of gamma-globulins by a mean factor of 4.4. Similar relationships between COP and protein were observed in the other species. Consequently, for a given total protein concentration, COP was dependent on the albumin/gamma-globulins ratio. A commonly used nomogram for estimating COP from protein concentration, the Landis-Pappenheimer equ...
Distribution studies of theophylline: microdialysis in rat and horse and whole body autoradiography in rat. After intravenous administration of theophylline, microdialysis has been used for studying the non protein bound theophylline concentration in blood and in lung tissue in the rat as well as in two horses. The distribution pattern of 14C-theophylline in the rat was also investigated. When the distribution of theophylline was completed the time course of free drug in the interstitial fluid in lung tissue was in good agreement with the total concentration-time profile in plasma in both species. In the rat the free concentration of theophylline in the lung was slightly lower than the free concentr...
Reduction and reoxidation of equine gonadotropin alpha-subunits. Ovine (o) and equine (e) LH alpha-subunits were reduced and reoxidized using conditions known to be effective for bovine and human alpha-subunits. The major product of oLH alpha refolding was alpha-subunit monomer. In contrast, eLH alpha formed a 121,000 mol wt aggregate. Monomeric eLH alpha was recovered, but in greatly reduced yield. To test the effects of carbohydrate variation on the aggregation of equine alpha-subunits, all of the equine gonadotropin alpha-subunits (eFSH alpha, eCG alpha, eLH alpha, and free alpha-subunit) were reduced and reoxidized. In each case, the major product was t...
Presence of pulmonary intravascular macrophages in the equine lung: some structuro-functional properties. The pulmonary intravascular macrophages (PIMs) have been described in several species of animals. This study demonstrates for the first time that the equine lung has PIMs as resident phagocytes in its microvasculature. Their salient features such as globular surface coat, structures of the endocytic pathway, and related cell organelles closely resemble those of the calf, goat, and sheep. The exquisite organization of the coat globules in the form of a linear chain was structurally similar to the lipolytic lipase and the heparin-sensitive globular coat from PIMs of calf, goat, and sheep. Monast...
Exogenous but not endogenous PGE2 modulates pony tracheal smooth muscle contractions. The modulatory role of prostaglandin E2 (PGE2) was examined in pony tracheal smooth muscle strips. Although exogenous PGE2 inhibited the contractile response to both electrical field stimulation (EFS) and acetylcholine (ACh) in a dose-dependent manner, the concentration required to inhibit the response to EFS (10 nM) was less than that required to inhibit the response to ACh (0.1 microM). Cyclooxygenase inhibition with aspirin or meclofenamate had no effect on either the response to EFS or to ACh even though PGE2 production was inhibited. Our results demonstrate that in ponies as in other spec...
Characterization of equine zona pellucida glycoproteins by polyacrylamide gel electrophoresis and immunological techniques. This study was designed to explore the composition of the equine zona pellucida (EZP) by one- and two-dimensional polyacrylamide gel electrophoresis (1D- and 2D-PAGE), silver staining and immunoblotting techniques. Antral follicles palpable on frozen-thawed equine ovaries were aspirated with a needle and syringe, and the resultant follicular fluid, cellular material and oocytes were pooled. Oocytes were placed in Petri dishes, moved by narrow-bore pipette to droplets of phosphate-buffered saline (PBS) and mechanically cleaned of cumulus cells. The EZP from these collected oocytes was solubiliz...
Dose-response effects of gonadotropin-releasing hormone on plasma concentrations of gonadotropins and testosterone in fertile and subfertile stallions. Five fertile and five subfertile stallions were treated with a single intravenous injection of saline the first week followed by a single intravenous injection of varying doses of gonadotropin-releasing hormone (5, 10, 25, 100, 500 micrograms) given in a randomized fashion over the next 5 weeks during the nonbreeding season. Blood samples were collected periodically before and after treatment for analysis of luteinizing hormone, follicle stimulating hormone, and testosterone content by radioimmunoassay. Before treatment, semen samples were collected every other day for 3 weeks for analysis of ...
[Comparison of IgG determination in foals using commercially available rapid tests]. The three tests (EQUI Z-Test, AGLUTINADE FOAL IMMUNITY, CITE Foal IgG-Test) were evaluated for their accuracy and usefulness in the field. Single radial immunodiffusion was used as reference method. All tests were easily and rapid to perform and results were obtained within a few minutes. It was easy to get the results of the CITE Foal IgG-Test, but use of the EQUI Z-Test and the FOAL AGLUTINADE IMMUNITY-Test needed some practice to get correct results. Results obtained by the CITE Foal IgG-Test correlated to single radial immunodiffusion in 94%, those obtained by FOAL AGLUTINADE IMMUNITY-Test...
Equine herpesvirus 5: comparisons with EHV2 (equine cytomegalovirus), cloning, and mapping of a new equine herpesvirus with a novel genome structure. A new equine herpesvirus, provisionally designated equine herpesvirus 5 (EHV5; Browning and Studdert (1987) J. Gen. Virol. 68, 1441-1447), was examined for the degree of genomic difference from equine herpesvirus 2 (EHV2) by Southern hybridizations. EHV5 and EHV2 whole genomic DNA probes were highly specific for homologous DNA only, indicating that significant genomic difference exists between the two viruses. Restriction endonuclease analysis of EHV5 strain 2-141 (EHV5.2-141) revealed that the genome is 179 kb and exists as a single isomer. Clones representing 82% of the genome were obtained ...
Cross-reactivity between a monoclonal antibody that recognizes a tumor-associated antigen on bovine lymphosarcoma cells and blood lymphocytes from various mammalian species. Tumor-associated antigens that are expressed in lymphosarcoma B cells of cattle with enzootic bovine leukosis had been analyzed in terms of their reactivity with 13 monoclonal antibodies (MAB). By use of flow cytometry and radioimmunoprecipitation, 1 of the MAB (c143) that recognized a tumor-associated antigen cross-reacted with blood lymphocytes (BL) from various mammalian species. By use of flow cytometry, the c143 MAB reacted with 10 to 49% of BL derived from human beings, mice, dogs, horses, pigs, llamas, sheep, goats, and cattle. Titer of the c143 MAB with BL from horses, pigs, human bein...
Anatomy of the prepubic tendon in the horse, cow, sheep, goat, and dog. Analyses of the fibers in the prepubic tendon of the horse and ruminants have shown that it is composed of the crossed and uncrossed tendons of origin of the pectineus muscles, the pelvic tendons of the rectus and obliquus abdominis muscles, and the tendons of origin of the cranial parts of the gracilis muscles. Pelvic attachments of the linea alba and the yellow abdominal tunic are incorporated in it. It is not a transverse ligament, and it is not homologous to the human superior (cranial) pubic ligament. The dog differs in 4 respects: (1) the pectineus tendons do not cross, but each originat...
Comparison of lidocaine, xylazine, and xylazine/lidocaine for caudal epidural analgesia in horses. Caudal epidural analgesia was achieved in 6 adult horses on 3 successive occasions at weekly intervals by injection of lidocaine, xylazine, and a combination of lidocaine/xylazine through indwelling epidural catheters. Analgesia was defined as a lack of response to pinprick and hemostat pressure in the skin of the perineal area. A significant (P < 0.05) difference was not found for time of onset of analgesia between lidocaine (4.3 +/- 0.8 minutes, mean +/- SEM) and the lidocaine/xylazine combination (5.3 +/- 1.3 minutes). Time to onset of analgesia after administration of xylazine was signi...
Low energy laser irradiation treatment for second intention wound healing in horses. Low energy helium-neon laser irradiation was administered to full thickness skin wounds (3 cm x 3 cm) on the dorsal surface of the metacarpophalangeal/metatarsophalangeal joints and cranial surface of the tarsocrural joints of eight horses. The effects on wound healing were analyzed statistically. There were no differences (p > 0.55) observed in the rate of wound healing between the low energy laser irradiated wounds and the control wounds. There was a significant difference (p < 0.006) observed in the rate of healing between the anatomical sites. Tarsal wounds healed more rapidly than fetlock...
Measurement of the cytotoxic effects of different strains of Mycoplasma equigenitalium on the equine uterine tube using a calmodulin assay. The cytopathic effects induced by five strains of Mycoplasma equigenitalium for cells of equine uterine tube explants were tested by measuring changes in cellular and extracellular concentrations of calmodulin (CaM). Calmodulin concentrations in samples of total homogenate (TH) and total homogenate supernates (THS) of the infected equine uterine tube explants were significantly lower than respective measurements on noninfected controls. In tissue culture medium fractions (TCM) of some infected explants, CaM concentrations were significantly higher than noninfected controls (p > 0.95). The r...
