Correlation analysis involves the statistical examination of relationships between variables to determine whether and how strongly they are associated. In equine research, correlation analysis is often used to explore the relationships between various physiological, behavioral, and environmental factors affecting horses. This method helps identify potential associations between variables such as diet, exercise, genetic markers, and health outcomes. By examining these relationships, researchers can gain insights into complex interactions and contribute to the understanding of equine biology and management. This page compiles peer-reviewed research studies and scholarly articles that focus on the application of correlation analysis in the context of equine science, highlighting its role in identifying patterns and informing decision-making in horse care and management.
Nambo Y, Nagata S, Oikawa M, Yoshihara T, Tsunoda N, Kohsaka T, Taniyama H, Watanabe G, Taya K.Plasma concentrations of immunoreactive (ir)-inhibin were measured in seven pregnant mares from around Day 140 of gestation to Day 2 after parturition using a heterologous bovine-based radioimmunoassay (RIA). Concentrations of luteinizing hormone (LH), follicle-stimulating hormone (FSH), oestradiol-17 beta, progesterone and relaxin were also measured in the same samples. A marked increase in plasma concentrations of ir-inhibin, FSH and LH occurred between Day 220 and Day 300 of gestation but the concentrations of all three hormones returned to baseline by about Day 320 (three weeks before part...
Mizobe M, Kondo F, Kumamoto K, Terada T, Nasu H.Rapid and quantitative analytical methods for bilirubin using high-performance liquid chromatography (HPLC) with UV detection were developed for samples from equines at a meat inspection site. Sharp HPLC peaks for bilirubins, unconjugated bilirubin (UCBL) and conjugated bilirubin (CBL), were obtained using a simple mobile phase of methanol:0.5 M Tris-HCl buffer (65:35, v/v, pH 7.4). A variable wavelength detector set at 450 nm, 0.01 AUFS and a recorder set at 4 cm/min were used for detection. Peaks for UCBL and CBL occurred at 7.1 min and 4.9 min, the lower limits of detection ranged between 0...
Ozawa A, Inokuma H, Johke T.The relationship between plasma insulin-like growth factor-I (IGF-I) level and body weight was studied in six breeds of horse. The correlation coefficients between plasma IGF-I and body weight in males and females of 1 year old heavy horses (Percheron and Breton breeds) were 0.62 and 0.12 respectively. The mean plasma IGF-I of males was higher than that of females (p < 0.01). However, the plasma IGF-I levels of heavy horses did not exceed those of light horses (Thoroughbred) or ponies (Shetland and Falabella). These data suggest that IGF-I is at least related to the difference of body weights ...
Geiser DR, Andrews FM, Rohrbach BW, White SL, Maykuth PL, Green EM, Provenza MK.Blood ionized calcium (Ca2+) and pH; plasma lactate concentrations; and total protein, total calcium (CaT), albumin, and phosphorus concentrations in serum were determined in 40 healthy horses before (T1), at the finish line (T2), and 10 minutes after the finish (T3) of the cross-country phase of a 3-day-event competition. Mean (+/- SEM) Ca2+ concentrations decreased from 6.22 +/- 0.04 mg/dl at T1 to 5.04 +/- 0.07 mg/dl at T2 (P < or = 0.05). This decrease was accompanied by a nonsignificant increase in CaT between T1 and T2. The mean (+/- SEM) percent ionization of calcium decreased significa...
Gillis C, Sharkey N, Stover SM, Pool RR, Meagher DM, Willits N.Results of studies in human beings and other species have indicated that aging significantly influences the strength, modulus of elasticity, and energy storage ability of tendon. We wanted to determine the effects of aging on the material and ultrasonographic properties of equine superficial digital flexor (SDF) tendon. Ultrasonographic measurements of left forelimb SDF tendon cross-sectional area and mean echogenicity were made in 23 standing horses ranging in age from 2 to 23 years. All horses had not been in work for a minimum of 6 months prior to the study. After euthanasia, left forelimb ...
Kaseda Y, Khalil AM, Ogawa H.The stability of relationships between harem stallions and mares (consort relations) was investigated and the durations of inter partum intervals were determined in order to establish if there was any correlation between the stability of consort relation and reproductive success of mares in Misaki feral horses. Thirty-four mares were observed continuously for more than 5 years. The lifetime stability was 80-100% (mean 92.4%) for 16 mares, 60-79% (mean 70.4%) for 10 mares and 0-59% (mean 27.9%) for 8 mares. The continuous length (years) of specific consort relations was 2-10 years and was found...
Clément F, Barrey E.The heart rate fluctuations at rest were studied in order to explore the emotionality of the horses by isolating the influence of the autonomic control. This paper presents a method of spectral analysis which was used to analyse the heart rate variability in the frequency domain. The heartbeat intervals were recorded during 1 h and a series of 1,024 heartbeats was extracted to compute a power spectrum of density. This was obtained by calculating the Fourier transform of the autocorrelation function of the series. This spectral analysis was applied to heart rate recordings in order to illustrat...
McCann ME, Watson TD, Boudinot FD, Moore JN.We evaluated the pharmacokinetics of IV administered sodium heparin and the pharmacodynamic effect of heparin on lipoprotein lipase (LPL) activity. Horses were allotted to 3 groups. Plasma samples were obtained from each horse before and at various times for 6 hours after heparin administration for determination of heparin concentration, LPL activity, and activated partial thromboplastin time (APTT). The disposition of heparin was dose dependent. The area under the plasma heparin concentration vs time curve (AUC) increased more than proportionally with dose, indicating that heparin elimination...
Richardson JD, Cripps PJ, Lane JG.Dental features are commonly expected to provide an accurate estimate of a horse's age. In this study the dentition of 434 thoroughbreds was photographed and the individual dental features documented, the true age of all the horses was known. For each dental feature the correlation with true age was determined. The eruption of the incisor teeth was the most consistent feature but it was not totally reliable. The presence of a hook on the upper corner incisor and Galvayne's groove proved to be of no value when estimating age and, of the attritional features studied, the dental star showed the h...
Ayala I, Montes A, Bernal LJ, Sandoval JA, Gutierrez C.The duration of electrocardiograph wave forms and intervals were determined in 179 Spanish-bred (Andalusian) horses aged from 1 month to 17 years. The values were compared with those of other breeds, and the relationship between electrocardiographic data and age was examined. High correlation coefficients were found between PR, ST and QT intervals and the age of the horses, and an inverted relation between heart rate and age was found. A multiple range analysis was made and the results suggest that significant changes in duration values and heart rate occurred at the age of 6 months and in the...
Price JS, Jackson B, Eastell R, Goodship AE, Blumsohn A, Wright I, Stoneham S, Lanyon LE, Russell RG.Biochemical markers of bone metabolism were analysed in serum samples obtained from 60 horses with no history of orthopaedic disease (age 3 months-20 years). Serum levels of the carboxyterminal propeptide of type I procollagen (PICP), a marker of bone formation and the pyridinoline cross linked telopeptide domain of type I collagen (ICTP), a putative marker of bone resorption, were measured by radioimmunoassay (RIA). Serum levels of the bone specific isoenzyme of alkaline phosphatase (BALP), another marker of bone formation, were measured by a wheatgerm agglutinin affinity (WGA) method. Total ...
Torres-Boggino F, Sato K, Oka A, Kanno Y, Hochi S, Oguri N, Braun J.Seminal characteristics, fertility and the response to semen preservation (liquid storage and cryopreservation) were evaluated in 4 Draft stallions (Percheron 2, Breton 2). Seminal characteristics (gel-free volume, sperm concentration, sperm morphology, percentage of motile spermatozoa) were assessed in 5 ejaculates from each of the 4 stallions. The fertility of the stallions was calculated retrospectively as the accumulated pregnancy rate over 3 breeding seasons. Five ejaculates from each of the stallions were subjected to liquid storage at 5 degrees C. The percentage of motile spermatozoa (P...
Barr AR, Dow SM, Goodship AE.Recordings of forelimb ground reaction forces were made from 48 normal ponies moving at the trot, to evaluate the relationships between bodyweight, vertical ground reaction forces, the timing of individual events within the stance phase and the total contact time. There were highly significant correlations between the mean vertical ground reaction forces and bodyweight. The mean vertical ground reaction forces, corrected for bodyweight, were inversely correlated with the total contact time suggesting that higher trotting speeds may be associated with greater vertical ground reaction forces. Th...
Evans DL, Polglaze KE.The presence of abnormal T waves in three or more electrocardiographic leads has been used to diagnose the cause of poor race performance in horses. This study investigated the relationship between previous racing performance in Standardbred horses and T waves, and the effect of training on the T wave. Thirty-two horses were electrocardiographed in two Sydney racing stables. Sixteen horses (50%) had ECGs with three or more leads with abnormal T waves, and these horses had won more races, had a greater ratio of wins per start and a greater number of dollars earned per start than horses with les...
Misumi K, Sakamoto H, Shimizu R.The purpose of this study was to investigate whether the change in the performance capacity of horses trained by running could be evaluated with a standardised swimming exercise test as well as by a standardised running exercise test. Seven two-year-old thoroughbred horses were trained by running for four months and were subjected to a standardised swimming exercise tolerance test before the training began and after two and four months of training in addition to the standardised running tolerance test after two and four months of training. The running training brought about a significant chang...
Milne EM, Pemberton AD, McGorum BC, Dixon PM, Scudamore CL, Miller HR.The concentration of alpha-1 proteinase inhibitor (API) was measured in the pulmonary epithelial lining fluid (PELF) of horses with chronic obstructive pulmonary disease (COPD) while they had clinical signs and while they had none. The concentrations of total protein, albumin and API were significantly higher in the PELF of animals with clinical signs of COPD. The correlation between albumin and API in the PELF suggested that most of the API was derived from the serum.
Alexander SL, Irvine CH, Donald RA.To characterize the short-term ACTH secretion pattern and to investigate factors regulating it, pituitary venous (PV) blood was collected using our nonsurgical method from 8 unperturbed horses every 20 or 30 s for approximately 1 h. In all but 1 horse, sampling occurred during the broad circadian maximum in plasma cortisol concentrations. Concentrations of corticotropin-releasing hormone (CRH; n = 7 horses), arginine vasopressin (AVP), ACTH and cortisol were measured by radioimmunoassay. In all horses, CRH, AVP and ACTH secretion patterns appeared irregular in time and amplitude. The mean (+/-...
Zhou J, Spier SJ, Beech J, Hoffman EP.It is often suggested that polygenic or environmental factors are responsible for clinical variability between patients with identical mutations. However, most dominant diseases are caused by a change-of-function alteration in the mutant allele's protein product. All patients are heterozygous and presumably express both mutant and normal proteins from the corresponding genes. Thus, a possible molecular mechanism for clinical variability could be the difference in relative levels of mutant vs. normal mRNA in different patients with the same mutation. To investigate this hypothesis, it is necess...
Richardson JD, Lane JG, Waldron KR.It is widely accepted that the age of a horse can be determined from an examination of its teeth, but there is no evidence that the accuracy of the systems of ageing used has been validated. A dental record was made of 80 horses of known age. There was a good correlation between the actual and apparent age of the horses up to five years, but older horses showed much greater variability and accuracy declined markedly after 11 years of age. The disappearance of the 'cup', but not the 'mark', proved to be one of the more reliable features. The average age at which the dental star appeared was one...
Attenburrow DP, Goss VA.The phase relationship between the periods of the respiratory and limb cycles is demonstrated in the horse ridden in the field at the canter and gallop. Changes in intra-abdominal pressure, respiratory sounds, periods of ground contact of each foot and volume changes of the rib cage were measured in the normal horse exercised at the walk, trot, canter and gallop in the field. Correlation of these parameters identifies the major mechanical link establishing the coupling of lung ventilation and locomotion in the horse. The force and extent of contraction of the abdominal muscles couples the resp...
Forhead AJ, French J, Ikin P, Fowler JN, Dobson H.Hyperinsulinaemia is a commonly-observed characteristic of insulin resistance, and a reduction in insulin sensitivity is thought to be either a causative and/or symptomatic feature of equine hyperlipaemia. A positive correlation (r = 0.545, P = 0.0015) existed between plasma insulin and triglyceride concentrations determined in 31 donkeys with naturally occurring hyperlipidaemia/hyperlipaemia. Greater insulin values tended to occur in the animals with an overweight body score. Inter-animal variation in insulin concentrations, however, prevented the identification of any differences either with...
Holyoak GR, Little TV, Vernon M, McCollum WH, Timoney PJ.Correlation between serum testosterone concentration and morphometric findings from ultrasonography of the accessory sex glands in peripubertal colts was investigated during pubertal development. Nineteen colts of initial age ranging from 5 to 12 months were monitored over a 13-month period. Serum testosterone concentration was determined on a biweekly basis, and accessory sex gland development was ultrasonographically monitored once a month. Notwithstanding individual variation, there was significant correlation (r = 0.913; P < 0.01) between increasing serum testosterone concentration and ...
Sommardahl CS, Andrews FM, Saxton AM, Geiser DR, Maykuth PL.Packed cell volume and plasma total protein (TP), serum albumin (Alb) and globulin (Glb), and plasma ionized calcium (PCa) concentrations, blood viscosity (BV), and plasma viscosity (PV) were measured in 42 horses at rest and after the cross country jumping phase of a horse trial competition. The BV and PV were determined at 6 shear rates (230, 115, 46, 23, 11.5, 5.75 s-1), using a digital rotational cone and plate microviscometer. A paired t-test was used to determine differences between PCV, TP, Alb, Glb and PCa values at rest and after exercise. The PCV, TP, Alb, and Glb values increased (P...
Johnson L, Carter GK, Varner DD, Taylor TS, Blanchard TL, Rembert MS.The number of Sertoli cells is important in spermatogenesis as noted by significant correlations between the number of Sertoli cells and the number of germ cells observed as early as type B2 spermatogonia in the horse. However, the stage within spermatocytogenesis at which these relationships first occur is unclear. The relationships between the number of Sertoli cells and parenchymal weight and the number of germ cells during the mitosis of spermatogenesis were determined in 184 adult horses to identify the developmental stage (that is, the earliest germ cell) at which significant relationshi...
Siciliano PD, Wood CH.Fourteen 2-yr-old Quarter Horses and Quarter Horse x Thoroughbreds were randomly assigned to either a control (CTRL) diet or a diet supplemented with 6.4% soybean oil (SBO). The amounts of both diets that were fed met current NRC nutrient requirements and were isoenergetic with similar nutrient:energy ratios. Venous blood samples (20 mL) were taken at d 0, 30, 60, and 90 of the experiment and analyzed for serum alpha-tocopherol, serum cholesterol, and serum triglyceride. The sum of serum cholesterol and serum triglyceride values was used as an estimate of serum total lipid. The ratio of serum ...
Warko G, Bostedt H.The aim of this study was to determine the course of the IgG concentration in blood serum of neonatal foals. A comparison of blood serum IgG concentration of the mares showed IgG values of 3356 +/- 671 mg/dl up to a maximum of 3579 +/- 766 mg/dl (means +/- s). In accordance with Eisenhauer (1981) and in contrast to Jeffcott (1974) no significant change of the IgG level in the blood serum of the mares could be observed during the test period. However, the IgG concentration in the colostrum of meanG = 11776 mg/dl during birth decreased considerably 96 hours later with meanG = 135 mg/dl. At birth...
Behrens E, Poteet B, Cohen N.The density of the cricoid cartilage from 29 equine larynges collected from an abattoir was determined by dual photon absorptiometry (DPA). Densities of the right and left cricoid cartilages were highly correlated. No correlation was found between age of the horse and the density of the cricoid cartilage.
Baker SJ, Gerring EL.Intragastric pH monitoring was investigated in ponies. In cadaver stomachs, close contact with the mucosa led to high pH readings if nonweighted electrodes were used. However, pH recorded by weighted electrodes was markedly less affected by mucosal contact (P < 0.001). The latter were used for subsequent trials. In vivo, high correlations were found between pH recorded by weighted electrodes with or without a wire guard to prevent mucosal contact (correlation, r = 0.866; P < 0.001). Readings from each correlated well with those from simultaneous gastric aspirates (r = 0.774 and r = 0.807...
Evans DL, Harris RC, Snow DH.At 2 and 5 mins after an 800-m gallop, venous blood was collected from 26 Thoroughbred racehorses for measurement of blood lactate concentration, packed cell volume (PCV) and haemoglobin concentration. In addition, 14 racehorses were given a strenuous submaximal treadmill exercise test. Heart rates during and after exercise at 10 m/sec on a treadmill inclined at 5 degrees were recorded. Blood samples at 2 and 5 mins after exercise were used to measure PCV, blood and plasma lactate and ammonia concentrations. Results of each exercise test were compared with the retrospective performance of hors...
Rathi R, Nielen M, Cheng FP, van Buiten A, Colenbrander B.Subfertility in stallions is attributed to the inability of spermatozoa to undergo the acrosome reaction in response to progesterone. In the present study, it was assessed whether there is a correlation between stallion fertility, defined on the basis of first cycle foaling rate and first cycle 'non-return rate', and the proportion of spermatozoa with exposed progesterone receptors on their plasma membranes. Semen from Dutch Warmblood (n=10) and Friesian (n=4) stallions was analysed. Progesterone 3-(o-carboxymethyl) oxime-BSA coupled with fluorescein isothiocyanate was used as a progesterone r...
Kyvsgaard NC, Høier R, Brück I, Nansen P.Ionizing irradiation and binary ethylenimine treatment have previously been shown to be effective for in-vitro inactivation of virus in biological material. In the present study the 2 methods were tested for possible effects on measurable concentrations of reproductive hormones in equine plasma (luteinizing hormone (LH), folliclestimulating hormone (FSH), progesterone (P4), and oestradiol-17 beta (E2)). The inactivation methods were electron beam irradiation with a dose from 11 to 44 kGy or treatment with binary ethylenimine (BEI) in concentrations of 1 and 5 mmol/L. Generally, there was a clo...
Wetmore LA, Derksen FJ, Blaze CA, Eyster GE.The relationship between mixed venous O2 tension and cardiac output was studied in six anesthetized horses breathing 100% O2. Cardiac output, O2 consumption, mean arterial pressure, heart rate, and arterial and venous blood gases were measured after administration of xylazine or dobutamine to horses in lateral, sternal, and dorsal recumbencies. After approximately 3 hours, Escherichia coli endotoxin was administered while horses were in dorsal recumbency, and all measurements were repeated. Relationships between cardiac index (CI) and PVO2, heart rate, mean arterial pressure, jugular PVO2, and...
Klecel W, Drobik-Czwarno W, Martyniuk E.Arabian show horses are well known for their exceptional beauty and elegance. The breed type, body conformation and movement are assessed during horse shows by licensed judges. The 5 judging categories are type, head and neck, body and topline, legs and movement, which are scored on a 20 point scale. It can be hypothesized that the scores in different categories are related to each other, and that the score for the most subjective type category depends on the scores for conformation categories. We analyzed 762 sets of average scores obtained by 583 unique horses at the World Championships. Cor...
Kaczmarek A, Nowak A, Leszczynski P.An increased occurrence of lifestyle-related diseases such as osteoporosis indicates the necessity for taking preventive action, including regularly engaging in physical activity. The aim of the study was to assess the areal bone mineral density (aBMD) and bone turnover markers levels in young adult women engaging in recreational horseback riding and to determine the relationship between training characteristics and bone metabolism indices. The study involved 43 women: 23 equestrians and 20 age- and body mass index-matched controls. The hip and spine aBMD and serum levels of the bone turnover ...
Naylor JM, Wolker RE, Pharr JW.Twenty students and 16 diplomates listened to 7 recordings made from 7 horses with either aortic (n = 3) or mitral valve (n = 4) regurgitant murmurs. A total of 30 different terms were used to describe the character of these murmurs. However, only 4 terms were used in a repeatable and consistent manner. Most people described the character of a given mitral or aortic valve murmur with 1 or 2 terms. Diplomates drew from a pool of terms that was about half the size of that used by students--8.1 +/- 2.0 terms for diplomats (mean +/- 1 SD) versus 13.1 +/- 1.8 terms for students (P > .001). Only blo...
Warko G, Bostedt H.The aim of this study was to determine the course of the IgG concentration in blood serum of neonatal foals. A comparison of blood serum IgG concentration of the mares showed IgG values of 3356 +/- 671 mg/dl up to a maximum of 3579 +/- 766 mg/dl (means +/- s). In accordance with Eisenhauer (1981) and in contrast to Jeffcott (1974) no significant change of the IgG level in the blood serum of the mares could be observed during the test period. However, the IgG concentration in the colostrum of meanG = 11776 mg/dl during birth decreased considerably 96 hours later with meanG = 135 mg/dl. At birth...
Baker LA, Burrows AM, Nonella KJ, Pipkin JL, Holmes LD, McEvers TJ, Tennant TC, Tisdale ZM, Voyles AH, Lawrence TE.Relationships between live body condition score (BCS) and carcass fat depots have not been well established in equine. Our study was designed to quantify the relationship between BCS and fat depot measurements from equine carcasses. Live horses ( = 429) were evaluated immediately prior to immobilization at a commercial equine processor. Horses were independently assigned a BCS by a panel of three trained evaluators; BCS was evaluated by visual appraisal and manual palpation of the neck, withers, back, ribs, behind the shoulder, and tailhead. Median BCS frequencies were: 3.0 ( = 9), 4.0 ( = 43)...
Draper ACE, Cahalan SD, Goodwin D, Perkins J, Piercy RJ.Equine recurrent laryngeal neuropathy (RLN) is a naturally occurring model of length-dependent axonopathy characterized by asymmetrical degeneration of recurrent laryngeal nerve axons (RLn). Distal RLn degeneration is marked, but it is unclear whether degeneration extends to include cell bodies (consistent with a neuronopathy). With examiners blinded to RLN severity, brainstem location, and side, we examined correlations between RLN severity (assessed using left distal RLn myelinated axon count) and histopathological features (including chromatolysis and glial responses) in the nucleus ambiguu...
Carstanjen B, Hoyle NR, Gabriel A, Hars O, Sandersen C, Amory H, Remy B.To evaluate a human assay for quantification of carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), assess the influence of age on plasma CTX-I concentration, investigate the relationship between plasma CTX-I and serum osteocalcin concentrations, and determine whether concentrations of plasma CTX-I or serum osteocalcin fluctuate in circadian manner in horses. HORSES: 75 clinically normal horses. Methods: Cross-reactivity between equine serum CTX-I and CTX-I antibodies in an automated electrochemiluminescent sandwich antibody assay (ECLIA) was evaluated via a specificity test...
Becker AC, Stock KF, Distl O.New movement traits reflecting unfavorable movement characteristics were defined on the basis of detailed movement evaluations (DME) of warmblood foals and mares performed in connection with regular breeding events of the Oldenburg horse breeding societies in 2009 and 2010. DME information was available for 3374 foals and 2844 mares and used for correlation analyses with conformation information on 1987 mares from studbook inspections (SBI) in 2009 and performance information on 2758 mares from mare performance tests (MPT) in 2000 to 2008. Analyses of variance revealed few significant differen...
Korosue K, Murase H, Sato F, Ishimaru M, Kotoyori Y, Nambo Y.The object of this study was to evaluate the usefulness of measuring the differences in the values of the serum total protein (DVSTP) concentration of foals and the refractometry index (DVRI) of the milk of dams before and after nursing of the colostrum for assessing failure of passive transfer (FPT) in foals. Serum samples from 31 foals were collected before the first nursing and other 1 to 6 times between 4 and 24 hr after birth. Paired colostrum and milk samples were collected from 14 of their dams at the same time. Serum samples were analyzed for IgG concentration using a single radial imm...
Cao XZ, Lin YZ, Li L, Jiang CG, Zhao LP, Lv XL, Zhou JH.The threshold hypothesis of attenuated lentiviral vaccine considers that the type of host response to infections of lentiviruses depends on the viral load. To evaluate the correlation between viral loads of the attenuated vaccine strain of equine infectious anemia virus (EIAV) and their effects to induce protective immunity, longitudinal plasma viral loads in groups of horses inoculated with either an attenuated EIAV vaccine strain (EIAV(DLV125)) or sub-lethal dose of an EIAV virulent strain (EIAV(LN40)) were compared. Similar levels of plasma viral loads ranging from 10(3)-10(5) copies/mL wer...
Ničová K, Bartošová J.This study aimed to identify factors that can influence show-jumping performance during top level competitions in the Western European League (2017/2018, CSI5*). The performance data of 144 riders and 222 horses were obtained from video records (FEI TV/ website). Riders with horses achieved a total of 9114 jumping-efforts over 320 obstacles including oxers (n = 142), oxers with water (n = 15), triple bars (n = 6), verticals (n = 136), verticals with water (n = 14) and walls (n = 7). Obstacles in the first round (FR) or in jump off (JO) were standing either as single (n = 6290) or as a combinat...
Suska M, Skotnicka E.The aim of this study was to examine the relationships between the concentrations of ATP, ADP, AMP (HPLC methods), total nucleotide pool (TAN), adenylate energy charge (AEC) and Na(+), K(+)-ATPase erythrocytic activities (by Choi's method) of horses as a function of breed and sex. The studies were conducted on 54 horses (stallions and mares) of different constitution types: breathing constitution (Wielkopolska and Hanoverian breed) and digestive constitution (Ardenian breed). Horse erythrocytes, independently of examined breed, present low ATP concentration in comparison to other mammal specie...
Ayala I, Montes A, Bernal LJ, Sandoval JA, Gutierrez C.The duration of electrocardiograph wave forms and intervals were determined in 179 Spanish-bred (Andalusian) horses aged from 1 month to 17 years. The values were compared with those of other breeds, and the relationship between electrocardiographic data and age was examined. High correlation coefficients were found between PR, ST and QT intervals and the age of the horses, and an inverted relation between heart rate and age was found. A multiple range analysis was made and the results suggest that significant changes in duration values and heart rate occurred at the age of 6 months and in the...
Linden A, Art T, Amory H, Desmecht D, Lekeux P.Seven sport horses were investigated to compare physiological and pharmacological stresses on the plasma glucocorticosteroid levels. Venous blood was sampled and analysed for cortisol concentrations before and after each type of stress. The physiological stress was induced by the completion of a cross-country and the pharmacological one, by an intravenous ACTH administration (200 micrograms) in the same horses, one week after the course. Both exercise and ACTH injection induced a highly significant cortisol increase of 79 +/- 0.10% and 160 +/- 0.18%, respectively. The relative plasma cortisol ...
Quiney LE, Ireland JL, Dyson SJ.There is good correlation between increased radiopharmaceutical uptake anywhere in the feet of sports or leisure horses and the presence of foot pain. However, low sensitivity of scintigraphy for identifying lesions contributing to foot pain as determined by magnetic resonance imaging (MRI) has been reported. The aim of this retrospective, cross-sectional, analytical study was to assess the accuracy of scintigraphy for identifying the cause(s) of foot pain, using MRI as the reference standard. Sports and leisure horses that underwent both skeletal scintigraphy and MRI of the front feet between...
Bohar Topolovec M, Kruljc P, Prošek M, Jazbec Križman P, Smidovnik A, Nemec Svete A.Coenzyme Q10 (CoQ10) is an essential cofactor in the mitochondrial electron transport pathway, and is also the only known endogenously synthesized lipid-soluble antioxidant. The aim of the present study was to determine, for the first time, endogenous plasma CoQ10 concentration and its correlation with plasma total antioxidant capacity (TAC) and serum total cholesterol (TC) in a population of healthy untrained horses. Thirty-one horses were included in the study. Plasma CoQ10 concentration ranged from 0.380 to 2.090 mg/L, which is in general agreement with plasma CoQ10 concentration in humans....
Vecchi I, Sabbioni A, Bigliardi E, Morini G, Ferrari L, Di Ciommo F, Superchi P, Parmigiani E.Standardbred maiden mares are generally stressed and in poor physical condition because of their incorrect management at the end of their racing careers. The purpose of this study was to identify an objective assessment that, similar to body condition score (BCS) determination, is easy to measure and able to confirm or improve fattening status assessment, as well as to ascertain whether a relationship with reproduction efficiency exists in subjects destined for a first-time insemination program. The authors assessed 29 Standardbred maiden mares (7 +/- 2 years old) during the breeding season. O...
Berryhill EH, Urbina NS, Marton S, Vernau W, Alonso FH.The Wellness Ready Test (WRT) is a lateral flow, stall-side assay that measures equine insulin in whole blood and requires validation before recommending clinical use. We evaluated intra- and inter-assay precision and linearity and compared the WRT with a radioimmunoassay (RIA). Tested concentrations ranged from 695 pmol/L (100 μIU/mL). For 20 replicates at each insulin level, intra-assay CVs of the WRT for insulin were 13.3%, 12.9%, and 15.3% at low (139-278 pmol/L; 20-40 μIU/mL), intermediate (278-417 pmol/L; 40-60 μIU/mL), and high (>417 >60 μIU/mL) concentrations...
Drum MG, Les CM, Park RD, Norrdin RW, McIlwraith CW, Kawcak CE.The purpose of this study was to compare subchondral bone density obtained using quantitative computed tomography with ash density values from intact equine joints, and to determine if there are measurable anatomic variations in mean subchondral bone density. Five adult equine metacarpophalangeal joints were scanned with computed tomography (CT), disarticulated, and four 1-cm(3) regions of interest (ROI) cut from the distal third metacarpal bone. Bone cubes were ashed, and percent mineralization and ash density were recorded. Three-dimensional models were created of the distal third metacarpal...
Satué K, Montesinos P, Muñoz A.In women and females of different species of laboratory animals, oestrogens stimulate the renin-angiotensin-aldosterone system (RAAS) by increasing tissue and circulating levels of angiotensinogen and renin during the preovulatory period. Progesterone and cortisol compete with aldosterone for mineralocorticoid receptors, which results in increased Na+ reabsorption during the postovulatory period. The purpose of the current research was to analyse the relationship of oestradiol-17β, progesterone and cortisol with RAAS in 23 mares during an oestrous cycle. During the preovulatory period, signif...
Catunda APN, Alves GES, Paes Leme FO, Carvalho AM, Leise BS, Johnson PJ, Faleiros RR.Inflammation and apoptosis in the hoof lamellar interface both contribute to the early stages of sepsis-associated laminitis, but it is not clear whether apoptosis is occurring before the onset of inflammation or is being provoked by inflammation. Apoptosis and inflammation were therefore measured in lamellar tissues obtained at different time points throughout the early stages of experimentally induced laminitis. Apoptotic cells and leukocyte were enumerated in archived paraffin embedded lamellar tissue samples from previous experiments in which acute laminitis was induced using Black Walnut ...
Tzelos T, Blissitt KJ, Clutton RE.To determine whether preoperative electrocardiographic measurements linked to sympathetic nervous activity could be used to predict recovery quality after general anaesthesia in horses. Methods: Prospective, clinical study. Methods: Eighteen adult client-owned horses. Methods: The electrocardiogram (ECG) was recorded presurgery in horses under three standard conditions: stabled unattended; with a groom whilst being led along a standard course; alone in the induction box. After surgery, each animal's recovery quality was scored by eight experienced anaesthetists or technicians using Donaldson's...
Arfuso F, Quartuccio M, Bazzano M, Fazio F, Piccione G.Knowledge of hematologic function in postparturient mares and foals is crucial for the monitoring of their health status and for the prompt diagnosis of pathologic conditions. Objective: The aim of this study was to evaluate erythrocyte osmotic fragility (EOF) and select hematologic variables in mares and their foals during the first month after foaling. Methods: Blood samples were collected from each animal every 3 days from day 1 until day 30 after foaling, and tested for RBC count, HGB, HCT, MCV, and EOF. Two-way repeated measures analysis of variance (ANOVA) and Bonferroni post hoc compari...
Williams NJ, Furr M, Navas de Solis C, Campolo A, Davis M, Lacombe VA.Metabolic syndrome in humans is commonly associated with cardiovascular dysfunction, including atrial fibrillation and left ventricular diastolic dysfunction. Although many differences exist between human and equine metabolic syndrome, both of these conditions share some degree of insulin resistance. The aims of this pilot study were to investigate the relationship between insulin sensitivity and cardiac function. Seven horses (five mares, two geldings, aged 17.2 ± 4.2 years, weight 524 ± 73 kg) underwent insulin-modified frequently sampled intravenous glucose tolerance testing to determine ...
Alexander SL, Irvine CH, Evans MJ.We used our nonsurgical technique for collecting pituitary venous blood to relate the dynamics of thyrotrophin-releasing hormone (TRH) secretion to the secretion patterns of both prolactin and thyrotrophin in periovulatory mares, either euthyroid (n = 5) or made hypothyroid by treatment with propyl-thiouracil (n = 5). Pituitary venous blood was collected continuously and divided into 1-min aliquots for 4 h. To test the effect of dopamine on the relationship between secretion patterns, sulpiride, a selective D2 receptor antagonist, was given i.m. after 2 h of sampling. Thorough testing of the m...
Holyoak GR, Little TV, Vernon M, McCollum WH, Timoney PJ.Correlation between serum testosterone concentration and morphometric findings from ultrasonography of the accessory sex glands in peripubertal colts was investigated during pubertal development. Nineteen colts of initial age ranging from 5 to 12 months were monitored over a 13-month period. Serum testosterone concentration was determined on a biweekly basis, and accessory sex gland development was ultrasonographically monitored once a month. Notwithstanding individual variation, there was significant correlation (r = 0.913; P < 0.01) between increasing serum testosterone concentration and ...
Wenzel RG, Major D, Hesp KF, Hall E, Doble P.To monitor cobalt concentrations in urine, red blood cells and plasma after chronic parenteral administration of cobalt chloride evaluate these results against the current International Federation of Horseracing Authorities thresholds for detecting cobalt misuse. Methods: Eight mares were randomly assigned to four treatment groups, with two mares in each group: Group 1 - control group, Group 2 - 25 milligrams cobalt intravenously as CoCl weekly, Group 3 - 50 milligrams cobalt intravenously as CoCl weekly, and Group 4 - 25 milligrams cobalt intravenously mid-week and at the end of the week. Uri...
