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Topic:Diagnostic Technique

Diagnostic techniques in equine medicine encompass a range of procedures and tools used to identify diseases, injuries, or other health conditions in horses. These techniques include imaging methods such as radiography, ultrasonography, and magnetic resonance imaging (MRI), as well as laboratory tests like blood work and tissue biopsies. Each diagnostic method provides specific information that can aid in the assessment and management of equine health issues. Radiography is commonly used for evaluating bone structures, while ultrasonography is useful for soft tissue examination. MRI offers detailed images of both soft and hard tissues, though its use is limited by cost and availability. Laboratory tests can detect biochemical and hematological changes indicative of disease. This page compiles peer-reviewed research studies and scholarly articles that explore the development, application, and efficacy of various diagnostic techniques in equine veterinary practice.
Evaluation of Protein Carbonyl Content in Healthy and Sick Hospitalized Horses.
Frontiers in veterinary science    October 27, 2020   Volume 7 582886 doi: 10.3389/fvets.2020.582886
Nocera I, Bonelli F, Meucci V, Rinnovati R, Spadari A, Intorre L, Pretti C, Sgorbini M.Literature on the protein carbonyl content (PCC) evaluation in horses is scarce, thus the aims were to evaluate the PCC in healthy and SIRS (Systemic Inflammatory Response Syndrome) horses and to investigate the performances of PCC in terms of sensitivity, specificity, and likelihood ratio in identifying SIRS positive and negative horses. A total of 72 adult horses were included. All the horses underwent to a complete physical examination, blood analysis, and were evaluated for the SIRS status. Blood samples were collected once in healthy horses and at admission time, then 24, 48, 72, and 96 h...
An Unusual Case of Testicular Disorder in Sex Development of Arabian Mare (64,XX SRY-Negative).
Animals : an open access journal from MDPI    October 25, 2020   Volume 10, Issue 11 1963 doi: 10.3390/ani10111963
Peretti V, Satué K, Ciotola F, Cristarella S, De Majo M, Biondi V, D'Anza E, Albarella S, Quartuccio M.A 3-year-old Arabian mare underwent medical examinations due to the presence of abnormalities of the reproductive apparatus and stallion behavior (nervous temperament, aggressiveness, masculine attitude). During the clinical visit, an anovulvar distance shorter than normal was observed; moreover, vulvar lips were dorsally fused except for the lower neckline, showing a blind ending from which a penis-like structure protruded. The ultrasound examination revealed the presence of a cervix and corpus of a uterus, hypoplastic uterine horns, and small gonads with an echogenicity similar to a testis. ...
Reference values for 2-dimensional and M-mode echocardiography in Friesian and Warmblood horses.
Journal of veterinary internal medicine    October 24, 2020   Volume 34, Issue 6 2701-2709 doi: 10.1111/jvim.15938
Vernemmen I, Vera L, Van Steenkiste G, van Loon G, Decloedt A.Echocardiographic reference intervals for Friesian horses are poorly described. Objective: To obtain reference intervals for echocardiographic measurements in Friesians and compare these with Warmbloods. Methods: One hundred healthy adult Friesians and 100 healthy adult Warmblood horses. Methods: Cross-sectional study. Two-dimensional and M-mode echocardiographic images were obtained. Echocardiographic measurements, including size, area, and volumetric measurements of left atrium, left and right ventricle, aorta, and pulmonary artery, were performed. Measurements were compared between the 2 br...
An investigation into factors influencing basal eye temperature in the domestic horse (Equus caballus) when measured using infrared thermography in field conditions.
Physiology & behavior    October 23, 2020   Volume 228 113218 doi: 10.1016/j.physbeh.2020.113218
Jansson A, Lindgren G, Velie BD, Solé M.Infrared thermography (IRT) is a popular technology used for the detection of thermal changes given its non-invasive nature and lack of direct contact with the individual. Accordingly, the maximal eye temperature (MaxET) measured with IRT has been extensively applied in equine research. However, there is little information available about the potential limitations of the MaxET in field studies. Thus, the aims of this study were to 1) quantify the individual variation of MaxET in field conditions and the effects of individual, breed, body size (height at withers), eye side, sex and age, 2) dete...
Molecular characterization of the re-emerging West Nile virus in avian species and equids in Israel, 2018, and pathological description of the disease.
Parasites & vectors    October 22, 2020   Volume 13, Issue 1 528 doi: 10.1186/s13071-020-04399-2
Schvartz G, Farnoushi Y, Berkowitz A, Edery N, Hahn S, Steinman A, Lublin A, Erster O.In this report we describe the molecular and pathological characteristics of West Nile virus (WNV) infection that occurred during the summer and fall of 2018 in avian species and equines. WNV is reported in Israel since the 1950s, with occasional outbreaks leading to significant morbidity and mortality in birds, high infection in horses and humans, and sporadic fatalities in humans. Methods: Animal and avian carcasses in a suitable condition were examined by post-mortem analysis. Tissue samples were examined for WNV by RT-qPCR and the viral load was quantified. Samples with sufficient material...
Non-weight bearing ultrasonographic examination allows the diagnosis of longitudinal fiber disruption (split) in equine suspensory ligament branches not visible on weight bearing examination. Werpy N, Chapman K, Griffith L.When evaluating suspensory ligament branch splits, there is a discrepancy between high field MRI findings and weight bearing ultrasonographic imaging characteristics. In this descriptive and retrospective case series, ultrasonographic examination was performed on suspensory ligament branches with the limbs in weight bearing and non-weight bearing positions. Suspensory ligament branch splits were defined as linear regions of decreased echogenicity when imaged with the limb in a weight bearing position that increased in size and became anechoic with the limb in a non-weight bearing position. Thi...
[Equine chorionic gonadotrophin: Biology and veterinary use].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    October 20, 2020   Volume 48, Issue 5 344-354 doi: 10.1055/a-1235-7973
Schuler G.The pituitary gonadotrophins follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play a prominent role in the control of gonadal functions. Therefore, their use in the treatment of fertility disorders (e. g. anovulatory anestrus) as well as in biotechnology (e. g. superovulation, hormone programs for cycle synchronization) is of substantial interest. Preparations of FSH or LH are relatively expensive due to the laborious extraction from pituitary tissue and are therefore reserved for special indications. In primates and equids, the chorionic epithelium expresses an LH-like molecule...
Serum symmetric dimethylarginine concentration in healthy horses and horses with acute kidney injury.
BMC veterinary research    October 20, 2020   Volume 16, Issue 1 396 doi: 10.1186/s12917-020-02621-y
Siwinska N, Zak A, Slowikowska M, Niedzwiedz A, Paslawska U.There are limited options to diagnose acute kidney injury (AKI) in horses. Symmetric dimethylarginine (SDMA) is routinely used in human and small animal medicine. The aim of this study was to assess serum SDMA concentrations in healthy horses and horses with AKI. The objective of this study was to evaluate the association of: 1) age, 2) sex, 3) body weight and 4) serum creatinine and urea levels on serum SDMA concentrations. Fifty-three healthy horses, including 17 foals (2-6 months of age) and 36 adult horses (3-29 years of age), and 23 horses with AKI were included in the study based on ...
Markers of long term silent carriers of Streptococcus equi ssp. equi in horses.
Journal of veterinary internal medicine    October 19, 2020   Volume 34, Issue 6 2751-2757 doi: 10.1111/jvim.15939
Pringle J, Venner M, Tscheschlok L, Waller AS, Riihimäki M.Difficulty in detection of silent carriers of Streptococcus equi is a key reason for its continued spread to immunologically naïve groups of horses. Objective: To determine whether clinical examination, markers of inflammation, or serology differentiate silent carriers of S. equi in recovered comingled horses. Methods: Ninety-eight warmblood yearlings and 72 unaffected mares on a large breeding farm (outbreak A), 38 mature Icelandic horses at a riding stable (outbreak B), and 27 mixed breed horses at a boarding stable (outbreak C). Methods: Prospective observational study 6 months to 2 year...
Diagnostic needle arthroscopy of the scapulohumeral joint in standing sedated horses.
Veterinary surgery : VS    October 19, 2020   Volume 50, Issue 1 29-37 doi: 10.1111/vsu.13529
Pouyet M, Bonilla AG.To describe the technique to perform diagnostic standing scapulohumeral joint needle arthroscopy with a 1.2-mm-diameter arthroscope in horses. Methods: Experimental study. Methods: Eight thoracic limbs in phase 1 and six horses in phase 2. Methods: In phase 1, the feasibility of the technique was evaluated by using a craniolateral arthroscopic approach. An evaluation of the visible structures of the scapulohumeral joint was performed with both a needle arthroscope and a 4-mm-diameter arthroscope. In phase 2, the technique was performed in six healthy sedated horses to validate the technique in...
Radiographic parameters of the digit in a cohort population of Amiata donkeys.
Open veterinary journal    October 19, 2020   Volume 10, Issue 4 354-362 doi: 10.4314/ovj.v10i4.1
The most common musculoskeletal conditions reported in donkeys are related to the foot. Radiographic examinations are clinically important in the diagnosis of foot abnormalities and are commonly used. However, few studies have been conducted to establish the normal radiographic appearance of a donkey's foot. To determine the radiographic features of the front digit in healthy Amiata donkeys. Radiographic examinations were performed on 56 forefeet of 28 Amiata donkeys. Three radiographic views of each front foot were taken: lateromedial, dorsopalmar and dorso-65°proximal/palmarodistal oblique....
Comparative Analysis of Intestinal Helminth Infections in Colic and Non-Colic Control Equine Patients.
Animals : an open access journal from MDPI    October 19, 2020   Volume 10, Issue 10 doi: 10.3390/ani10101916
Gehlen H, Wulke N, Ertelt A, Nielsen MK, Morelli S, Traversa D, Merle R, Wilson D, Samson-Himmelstjerna GV.All around the world, intestinal helminths constitute one of the most prevalent life-long occurring infections and re-infections affecting all horse age groups. A range of parasite species among strongyles, ascarids, and tapeworms is known to have the potential to cause colic in horses. However, there is a lack of current scientific evidence on the actual relevance of helminth infection levels in the context of colic in horses kept during prevailing epidemiological conditions. Thus, a prospective case-control study on the occurrence of intestinal helminths in a total of 620 mainly adult equine...
Evaluation of podocin in urine in horses using qualitative and quantitative methods.
PloS one    October 15, 2020   Volume 15, Issue 10 e0240586 doi: 10.1371/journal.pone.0240586
Siwińska N, Pasławska U, Bąchor R, Szczepankiewicz B, Żak A, Grocholska P, Szewczuk Z.No sensitive method for diagnosing early kidney dysfunction in horses has been identified so far. Many studies carried out in humans and small animals show that podocin can be useful to diagnose various kidney diseases, mainly affecting the glomeruli. The aim of this study was to perform a qualitative and quantitative analysis of podocin in urine samples obtained from healthy horses, horses with clinical kidney dysfunction and horses at risk of acute kidney injury. The study objectives aimed to assess: (1) whether the selected podocin tryptic peptide for LC-MS-MRM allows for podocin detection ...
Equine Sinus Surgery.
The Veterinary clinics of North America. Equine practice    October 14, 2020   Volume 36, Issue 3 613-639 doi: 10.1016/j.cveq.2020.08.003
Pigott J.The anatomy of the equine paranasal sinuses is critical to understand to assess the extent of the disease process, the optimal surgical approach, and the ability to drain through the normal nasomaxillary pathway. By following established anatomic landmarks, direct sinus access can be used to further explore the sinus compartments, remove compromised cheek teeth, remove any purulent debris or feed contamination, and establish drainage. Many complications can be avoided or minimized by thoroughly evaluating all sinus compartments and critically assessing the ability of the sinus to drain.
Dental Radiography and Radiographic Signs of Equine Dental Disease.
The Veterinary clinics of North America. Equine practice    October 14, 2020   Volume 36, Issue 3 445-476 doi: 10.1016/j.cveq.2020.08.001
Baratt RM.Radiography is an important imaging modality and is available to most equine veterinarians providing primary care. Diagnostic radiographic imaging of the equine skull and dentition requires careful positioning and technique. This article is aimed at providing the veterinarian with instructions and guidelines for obtaining diagnostic skull and dental radiographs and a discussion of the radiographic signs of dental disease with case-based examples. The limitations of radiography are discussed with regard to determining the need for adjunct diagnostic techniques and more advanced imaging techniqu...
Equine Oral Endoscopy.
The Veterinary clinics of North America. Equine practice    October 14, 2020   Volume 36, Issue 3 433-443 doi: 10.1016/j.cveq.2020.07.001
True CK, Dotzel AR.Oral endoscopy is a valuable addition to the equine dental examination process. It enables veterinarians to visualize subtle oral disorders and is a useful client education tool. There are several commercially available oral endoscopic systems on the market. Practitioners can also assemble their own systems. An oral endoscope is used to perform a thorough and systematic oral examination and to visualize normal oral structures as well as oral disorders. It is also used to guide instrument placement during oral surgeries and other dental procedures.
Equine Imaging: Computed Tomography Interpretation.
The Veterinary clinics of North America. Equine practice    October 14, 2020   Volume 36, Issue 3 527-543 doi: 10.1016/j.cveq.2020.08.007
Epperly E, Whitty JA.Computed tomography (CT) has revolutionized the veterinarian's ability to image the equine skull and led to improved diagnostic accuracy and clarity for surgical planning. The increased cost for this evaluation is offset by more accurate diagnosis and targeted therapy. As novel technology is developed that allows for increased availability of equine head, the price will continue to decrease and more examinations will be performed. New skills are needed for the veterinarian to accurately interpret this modality. This article reviews the normal CT appearance of the equine skull and presents exam...
Equine Nasal Endoscopy: Treating Bullae Disease and Sinus Disease.
The Veterinary clinics of North America. Equine practice    October 14, 2020   Volume 36, Issue 3 659-669 doi: 10.1016/j.cveq.2020.08.005
Claffey EF, Ducharme NG.The nasal conchal bullae (dorsal and ventral) are separate, air-filled structures within their respective dorsal and ventral nasal conchae. Computed tomography scans have assisted with the increasing diagnosis of empyema of the nasal conchae. This condition is usually associated with dental or sinus disease. Drainage of affected bullae is considered critical for resolution of clinical signs. The ventral conchal bullae can be easily viewed with a standard 10 mm diameter flexible endoscopy via the middle nasal meatus. This approach can also be used for fenestration of the bullae, using a diode ...
A duplex qPCR assay for human erythropoietin (EPO) transgene to control gene doping in horses.
Drug testing and analysis    October 14, 2020   Volume 13, Issue 1 113-121 doi: 10.1002/dta.2907
Cheung HW, Wong KS, Lin VYC, Wan TSM, Ho ENM.The misuse of genetic manipulation technology to enhance athletic performance is termed gene doping which is prohibited in human sports, horseracing, and equestrian sports. Although many qPCR assays have been developed, most assays employ genomic DNA (gDNA) from humans, non-human primates, and mice as a background and they may not be applicable for testing horse samples. This study aimed to develop a qPCR assay for the detection of human erythropoietin (hEPO) transgene in horse blood cells where the viral vectors used in gene therapy can reside for months. For the detection of hEPO transgene, ...
Improvement of the clinical signs of gait abnormality after treatment with levothyroxine in a horse with shivering and hypothyroidism.
Acta veterinaria Hungarica    October 13, 2020   Volume 68, Issue 2 147-153 doi: 10.1556/004.2020.00026
Saadi A, Dalir-Naghadeh B, Akbari H, Rashedi M, Mohammadi R.An 11-year-old Hanoverian gelding used for jumping was evaluated for gait abnormalities and hoof problems in the hindlimbs. Clinical examinations revealed signs consistent with shivers. A thyroid gland enlargement was noticed, baseline serum thyroid hormone (TH) concentrations were low, and a low response to thyrotropin-releasing hormone administration was observed. Hypothyroidism was suspected. The horse was treated with levothyroxine for 1 year. TH concentrations returned to the normal range by week 4 of treatment. Thirty weeks after the initiation of levothyroxine therapy, the gait abnormal...
Meta-analysis of cyathostomin species-specific prevalence and relative abundance in domestic horses from 1975-2020: emphasis on geographical region and specimen collection method.
Parasites & vectors    October 12, 2020   Volume 13, Issue 1 509 doi: 10.1186/s13071-020-04396-5
Bellaw JL, Nielsen MK.Cyathostomins infect virtually all horses, and concomitant infections with 10 or more species per horse is standard. Species-specific knowledge is limited, despite potential species bias in development of disease and anthelmintic resistance. This is the first meta-analysis to examine effects of geographical region and cyathostomin collection method on reported composition of cyathostomin communities. Methods: Thirty-seven articles published in English in 1975 or later, in which adults of individual species were systematically enumerated, were included. Seven regions; North America, South Ameri...
Ultrasonographic evaluation of the width, thickness, and length of the normal linea alba in standing and dorsal recumbent horses.
Veterinary surgery : VS    October 12, 2020   Volume 50, Issue 1 158-169 doi: 10.1111/vsu.13528
Verkade ME, Suthers J, Wiemer P, Martens A, De Clercq E, Burford J.To determine the variability in length, width, and thickness of the equine linea alba (LA) and the effect of a standing vs dorsal recumbent position on these measurements. Methods: Descriptive anatomical comparative study. Methods: Standing horses (N = 75; in 30 horses, measurements were obtained in dorsal recumbency first and repeated after horses were standing). Methods: Linea alba length was measured in standing position from xiphoid to umbilicus, and transverse ultrasonographic images were obtained at five reference points to measure LA width and thickness. In 30 horses, measurements were ...
Furosemide administration onehour before bone scintigraphy examination in horses does not improve the image quality or reduce the radiation dose rate. Mageed M, Wegert J, Dyab S, Gerlach K.This prospective, cross-sectional, pilot study aimed to investigate the effects of furosemide as a diuretic on the image quality of bone scintigraphy performed using Tc-HDP and to investigate the impact of furosemide on the radiation dose rate. Thirty-one horses undergoing bone scintigraphy were included. The horses were divided into the control (n = 14) and furosemide group (n = 17), which received 1 mg/kg furosemide intravenously 1 h post Tc-HDP administration. The image quality was assessed subjectively and semi-quantitatively. The bone-to-soft tissue (B:S) ratio was calculated from...
Differentiation of S. chartarum (Ehrenb.) S. Hughes Chemotypes A and S via FT-IR Spectroscopy.
Mycopathologia    October 10, 2020   Volume 185, Issue 6 993-1004 doi: 10.1007/s11046-020-00495-0
Ekruth J, Gottschalk C, Ulrich S, Gareis M, Schwaiger K.Stachybotrys (S.) chartarum is a cellulolytic mould with the ability to produce highly cytotoxic macrocyclic trichothecenes. Two chemotypes are defined according to their ability to produce either atranones or satratoxins. S. chartarum has been well known as the causative agent of the lethal disease stachybotryotoxicosis in horses. Further investigations revealed that this disease is strictly correlated with the presence of macrocyclic trichothecenes. Furthermore, their occurrence in water-damaged buildings has been linked to adverse health effects such as the sick building syndrome. As the ch...
Evaluation of the effects of medial saphenous venous pressures as an indirect method of measurement of intra-abdominal pressures in the horse.
Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)    October 8, 2020   Volume 30, Issue 6 660-669 doi: 10.1111/vec.13000
Munsterman AS, Gillen AM, Coleridge MOD, Hanson RR.To evaluate the effect of changes in intra-abdominal pressure (IAP) on medial saphenous venous pressure (MSVP) and hemodynamics in normal horses. Methods: Experimental, in-vivo study. Methods: University Teaching Hospital. Methods: Convenience sample of 7 mixed breed horses; 5 geldings, and 2 mares. Methods: Pneumoperitoneum was induced in horses under standing sedation with carbon dioxide gas using a laparoscopic insufflator for a total of 60 minutes to simulate clinical elevation in IAP. Pressure was increased stepwise to 20 mm Hg over 30 minutes, and maintained at that pressure for 30 minu...
Validation of a Rapid Bronchoalveolar Lavage Fluid Colorimetric Evaluation for Assessing the Severity of Exercise-Induced Pulmonary Hemorrhage in Horses in Field Conditions.
Journal of equine veterinary science    October 8, 2020   Volume 95 103284 doi: 10.1016/j.jevs.2020.103284
Warlick LM, Léguillette R, Kogan C, Gold JR, Bayly WM.Diagnosis and assessment of severity of exercise-induced pulmonary hemorrhage (EIPH) relies on postexercise visualization of fresh blood in the airways via tracheobronchoscopic examination (TBE) and/or counting erythrocytes in bronchoalveolar lavage fluid (BALFRBC). Determining the BALFRBC is more sensitive than TBE but its usefulness is hampered by the need to have BALFRBC counted at a laboratory. We explored the feasibility of evaluating the severity of EIPH by using a color chart comprised of five shades of red and matching those colors with the color of BALF immediately following collectio...
Occurrence of Intrauterine Purulent Concrements in a Maiden Mare-A Case Report.
Journal of equine veterinary science    October 8, 2020   Volume 95 103278 doi: 10.1016/j.jevs.2020.103278
Köhne M, Tönissen A, Unruh C, Pruß D, Sieme H.Pyometra is an uncommon condition in mares associated with various symptoms. Here, we report a case of a 13-year-old Icelandic barren maiden mare with recurrent vaginal discharge. Ultrasonographically, the mare displayed intrauterine spherical masses of inhomogenous texture, which were identified as purulent concrements in hysteroscopy. The purulent concrements were successfully removed via uterine lavage after endoscope-assisted comminution. Microbiologic examination of the concrements revealed growth of Streptococcus equi subspecies zooepidemicus, Actinobacillus species, Pseudomonas aerugino...
An Epidemiological Study on Orodental Disorders in 3,791 Working Donkeys in Egypt: Prevalence and Risk Factors.
Journal of equine veterinary science    October 8, 2020   Volume 95 103274 doi: 10.1016/j.jevs.2020.103274
Serag RM, Abu-Seida AM, Abdelrahman HA, Samir A, Ibrahim IM, AbdElkader NA.Orodental disorders are common in equidae and can lead to serious clinical complications. This study determines the prevalence rate (PR) of orodental disorders in working donkeys in Egypt and their potential risk factors. During 4 years, 3,791 donkeys were examined in six Egyptian governorates. Full case history and thorough clinical and oral examinations were performed. Radiography and oroendoscopy were undertaken whenever possible. All data were statistically analyzed using Poisson generalized linear models to compare PR among governorates, sex, years, body condition score, and age groups an...
Radiographic findings and anatomical variations of the caudal cervical area in horses with neck pain and ataxia: case-control study on 116 horses.
The Veterinary record    October 8, 2020   Volume 187, Issue 9 e79 doi: 10.1136/vr.105756
Beccati F, Pepe M, Santinelli I, Gialletti R, Di Meo A, Romero JM.Abnormalities of the ventral lamina of the sixth cervical vertebra (AVL-C6) are thought to exert abnormal stress on the articular process joints (APJs) of the cervicothoracic junction. The aim of the study was to investigate the association between AVL-C6 and radiographic findings in the caudal cervical area and between clinical signs of neck pain and ataxia and radiographic findings. Methods: Medical records of horses subjected to cervical radiography were reviewed. Horses were classified into those with neck pain (group C), those with ataxia (group A) and healthy horses (group H). Presence o...
Epidemiology of epizootic lymphangitis of carthorses in northern Ethiopia using conventional diagnostic methods and nested polymerase chain reaction.
BMC veterinary research    October 7, 2020   Volume 16, Issue 1 375 doi: 10.1186/s12917-020-02582-2
Hadush B, Michaelay M, Menghistu HT, Abebe N, Genzebu AT, Bitsue HK, Afera B, Duguma BE, Gugsa G, Ameni G.Epizootic lymphangitis (EL), caused by Histoplasma capsulatum variety farciminosum (HCF) is a contagious, chronic disease of equines, characterized by development of nodular lesions in the lymph nodes, lymphatic vessels and skin. It is one of the most important diseases of equines in Ethiopia, causing significant economic loss, particularly in the livelihood of carthorse owners. To date there is neither effective diagnostic nor control measure implemented in the country. Furthermore, there is a shortage of data on the epidemiology of the disease in different regions of this country. The aim of...
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