Equine biomechanics involves the study of the mechanical principles that govern the movement and structure of horses. This field examines the anatomical and physiological aspects that contribute to locomotion, including gait patterns, joint function, and muscle dynamics. Researchers utilize advanced technologies such as motion capture and force plate analysis to assess the efficiency and performance of equine movement. Understanding biomechanics aids in optimizing training regimens, improving performance, and preventing injuries. This page compiles peer-reviewed research studies and scholarly articles that explore the mechanics of equine movement, its applications in sports and rehabilitation, and its impact on overall equine health.
Daniels S, Martin S, Harris P, Moore-Colyer M.The in vitro gas production system (GPS), developed to estimate degradation of ruminant feedstuffs, has been adapted for equine use. This study aimed to characterise the bacterial community profile and metabolome of donor faeces and faecal inoculum within the GPS when fermenting the same diet as faecal donors. Six Welsh ponies on identical diets were faecal donors with samples collected for microbiome profiling and system inoculation. Gas production (manual pressure transducer technique) was performed for 156 h with 2 replicate bottles from each donor harvested at 8,20,28 and 36 h. Faecal an...
Manolăchescu D, Tripon M, Crecan C, Tătaru M, Papuc I.Emotional contagion in human-horse interactions has been widely studied; however, the role of body language in stress transfer remains insufficiently explored. Unassigned: This study examines whether human emotional states, particularly stress and anxiety, influence horses and whether the transmission occurs primarily through body language. Unassigned: A repeated-measures within-subjects design was used. The order of interaction styles was randomized across subjects during 33 HCIs. Participants were categorized as high-anxiety (HA) or low-anxiety (LA) based on State Anxiety Scale scores and he...
Bolesławska-Szubartowska J, Kucharczuk M, Skrabska A, Zbysław A, Adamowicz J, Alszko A, Domagalska-Stomska K, Durska M, Dziekcierów A....Pain after giving birth is commonly observed in horses, yet there has not been a specific tool developed for assessing this pain in postpartum mares. The goal was to adapt existing equine pain scales and to preliminarily validate a practical pain scale for use by veterinarians and caregivers after foaling. Methods: The pain scale was developed by adapting items from other pain scales, including established orthopedic and colic equine pain scales, and incorporating caregiver feedback. The final scale includes eight areas for assessing pain: behavior, facial expressions, vital signs, udder exami...
Mohtasebi S, Ahn S, Karimi M, Saberi M, Gilleard JS, Poissant J.Strongyles pose significant health concerns for equids globally. Strongyles, comprising over 60 species, can lead to severe morbidity and mortality, with Strongylus vulgaris posing higher risks due to its migratory behaviour. Routine diagnostic methods, such as faecal egg counts, lack species-level resolution, while traditional morphological techniques require advanced expertise. DNA metabarcoding offers a high-throughput alternative. Objective: To characterise the diversity of strongyles infecting horses in northern Iran and evaluate how age, sex, diagnostic methods and host population influe...
Littiere TO, da Costa GB, de Sales NAA, de Carvalho JRG, Rodriguez IDM, Ramos GV, de Lacerda-Neto JC, Ferraz GC.Maximal lactate steady state (MLSS) and its predictive thresholds, including the lactate threshold (LT), are widely used to estimate endurance capacity, prescribe training, and assess the effects of training. The human literature identified approximately twenty-five methods to predict the MLSS. This communication compares the concordance between the two D methods and the MLSS. Under laboratory conditions, ten teaching horses underwent a submaximal graded exercise test (GXT) on a treadmill to obtain their lactate-velocity curves (LVC). They performed three to five 30-min continuous running sess...
Ludwig A, Biermann R, Frölich K, Jandowsky A, Langner C, Lieckfeldt D, Tschann K, Reissmann M.Domestic Baroque donkeys are popular animals in many European parks and zoos. Although their cream coat color is very charismatic, the underlying genetics is still undiscovered. Addressing this question, a candidate approach was used to search for the causative mutation.Considering the knowledge from domestic horses, the () was identified as the most promising candidate. Samples from 77 domesticated donkeys, 11 wild equids, 1 mule and 2 domestic horses were included in comparison. All exons and the flanking intron sequences of the were sequenced (2774bp).Sequence comparison revealed that a m...
Equine cognition is relevant to the many roles that horses serve in society, such as leisure riding, competitions, or even animal-assisted therapy. Equine cognitive abilities have been explored in recent years. However, gaining an overview of horse cognition is challenging due to the broad range of abilities studied and the diverse methodologies employed. In addition, the subjects of existing equine cognition studies vary greatly in contextual factors such as their breed, age, sex, and management conditions - each of which may influence test performance in the following cognitive categories: D...
Fouché N, Howard J, Gerber V, Billmann P, Farinha do Sul M, Christen G, Bruckmaier R, Philipona C, Schmutz NB, Gross J.β-endorphin, a proopiomelanocortin (POMC)-derived peptide secreted by pars intermedia melanotropes, may play a significant but underexplored role in pituitary pars intermedia dysfunction (PPID) pathophysiology and diagnosis. This study aimed to (1) validate a commercially available human β-endorphin enzyme-linked immunosorbent assay (ELISA) kit for equine samples, and (2) compare β-endorphin concentrations between horses with PPID and healthy controls. Assay validation included the generation of standard curves using purified synthetic equine β-endorphin and human β-endorphin standards, w...
Moghbeli Damane M, Ayatollahi Mehrgardi A, Esmailizadeh A, Momen M.Inbreeding is caused by mating between related individuals and is associated with reduced fitness and performance. Generally, in the horse population, inbreeding is caused by geographically restricted areas and intensive natural or artificial selection. For this reason, assessing accurate inbreeding is essential for developing and implementing effective breeding strategies aimed at preserving genetic diversity and reducing the harmful consequences of inbreeding. One of the most accurate approaches for assessing genomic inbreeding and autozygosity is through the analysis of runs of homozygosity...
Mańkowska A, Dobraczyńska BM, Szewczak J, Chodup Z, Radzanowski B, Matychyn I, Witkowska D.Cultivated carrot ( subsp. ) is a valuable component of equine diets due to its high β-carotene content (a precursor of vitamin A) and desirable sensory properties. However, its use may be limited by short shelf life, susceptibility to spoilage, and firm texture, which can be challenging for horses with dental problems. This study aimed to evaluate horses' preferences for different physical forms of carrots. Twenty-one leisure horses aged 3-22 years were individually evaluated in a free-choice test. Each horse was simultaneously offered equal portions (150 g) of five carrot forms: raw, grated...
Jula CA, Davis JL, McKenzie HC, Council-Troche M.To describe the pharmacokinetics of a single dose of pimobendan (Vetmedin) in healthy adult horses and provide preliminary evidence of the absorption of 2 compounded pimobendan formulations. Unassigned: From August 2022 through November 2022, 6 healthy adult horses were administered a single dose of 0.5 mg/kg pimobendan orally. A subset of 2 horses was also administered pimobendan, compounded as an oil-based suspension and a capsule formulation. Plasma was collected at times 0, 15, 30, and 45 minutes and 1, 2, 4, 8, 12, and 24 hours after administration. The plasma concentrations of pimobendan...
Stachurska A, Wnuk E, Łuszczyński J, Donderowicz W.Correct conformation and symmetry of bilateral hooves are associated with a properly balanced hoof, which has a significant impact on the biomechanics of movement and reduces the risk of injury. The aim of the study was to compare solear aspect dimensions of bilateral hooves in forelimbs (hoof width, length, frog width, length, medial and lateral diagonals) with regard to sex, breed and age of horses. The dimensions were measured with a calliper in 100 horses of four breeds. The results showed that the breed factor significantly affected all dimensions. Age was significant for some variables, ...
Stokes C, Natalini CC, Wills R, Eddy A, Mochal-King C, O'Shea C, Fontenot RL.To evaluate the analgesic properties and adverse effects of liposomal bupivacaine administered via caudal epidural in horses. Methods: Pharmacodynamic study. Methods: A total of 10 healthy adult horses. Methods: Liposomal bupivacaine (10 mL; 1.3%) was administered into the caudal epidural space via an indwelling epidural catheter inserted to a length of 15 cm. Mechanical nociceptive threshold (MNT) testing with pressure algometry was used to measure analgesia at four anatomic locations for 72 h following injection. Physical examinations (including rectal temperature, heart rate, respirat...
Alexeenko V, Anchan DS, Ter Woort F, Ribonnet C, van Erck E, Marr C, Jeevaratnam K.Non-linear equine electrocardiography (ECG) analysis is an actively developing study area which has the potential to lead to novel, artificial intelligence-based diagnostic tools in equine cardiology. As more ECG recording devices are becoming available, there is a need to ensure results are interchangeable regardless of the equipment used to record the equine ECG. Objective: To evaluate the agreement of ECG complexity values obtained using the Televet™ and Equimetre™ systems. Methods: Cross-sectional clinical. Methods: ECGs were recorded using two devices simultaneously from 37 healthy Th...
McCrae P, Spong H, Moorehead J, Pearson W.A smart textile device has been developed for the recording of electrocardiograms (ECGs) in horses; however, the utility of this device for long-duration heart rate variability (HRV) monitoring and detection of physiological arrhythmias is unknown. Therefore, the objective of this study was to validate a smart textile device for HRV over long durations (6 h) in resting horses. ECGs were recorded simultaneously via the Myant Skiin Equine textile device and a reference device (Televet 100) in 12 horses. ECGs were evaluated by a blinded observer for arrhythmias, and HRV metrics were calculated. ...
Jacobs ME, Blea J, Hardy M, McKemie DS, Traynham M, Knych HK.The racehorse industry has strict regulations regarding the detection of prohibited substances in horses. Metformin, a diabetes medication, is a prohibited substance that has been reported in post-race blood and urine samples collected from racehorses. For further characterization of the disposition of metformin, 12 Thoroughbred horses were administered metformin orally and intravenously in a randomized, balanced, two-way crossover design. Serum and urine samples were collected, and drug concentrations determined via liquid chromatography-tandem mass spectrometry. The serum data were analyzed ...
Morrice-West AV, Wong ASM, Hitchens PL, Whitton RC.Musculoskeletal injuries (MSI) in racehorses are not typically spontaneous events, but develop gradually through cumulative cycles of loading over time. Stride characteristics can now be recorded with wearable technology, providing opportunity to quantify the skeletal impact of galloping over time. We estimated bone fatigue accumulation based on speed (m/s) and number of strides from n = 3168 race starts by n = 405 Thoroughbreds commencing racing in Tasmania, Australia between 2011 and 2016. The percentage bone fatigue accumulated was divided by various time periods (career, active car...
Silva JA, Vasquetto A, Mouguelar H, Nader-Macías MEF, Pellegrino MS.Equine endometritis, a major cause of subfertility in mares, has a significant economic impact on horse breeding. In the face of rising antimicrobial resistance, the use of beneficial bacteria to prevent this disease is a non-antibiotic alternative which aligns with "One Health" principles. This study characterised vaginal autochthonous lactic acid bacteria (LAB) from healthy mares. Out of the 25 presumptive LAB isolates obtained from 15 clinically healthy thoroughbred mares, 24 were confirmed. Surface properties were assessed (autoaggregation, hydrophobicity, biofilm formation, exopolysacchar...
McPeek JL, Menarim B, Sponseller B, McClendon M, Adam EN, Adams AA, Slone S, Page AE.Subjective lameness evaluations are critical components of equine musculoskeletal health assessments. Objective approaches can supplement diagnosis and may be preferred for specific cases and scientific purposes. Objective: Evaluate agreements between subjective evaluation of two veterinarians and standard clinical interpretations from outputs of an AI-based smartphone application (Sleip; AI-SPA), the inertial measurement unit (IMU) system Equinosis Q Lameness Locator (LL), and the IMU system Equisym (ES). Methods: In vivo experiment. Methods: Twenty-five research horses (10-30 years) were e...
Mitlyng N, Hobbs KJ, Cooper BL, Sheats MK.Lipopolysaccharide (LPS) is frequently used in equine research to model clinical endotoxemia; however, there is no standardized protocol for inducing cytokine production in equine whole blood. To address this knowledge gap, the goal of this study was to compare the cytokine responses elicited by three different LPS stimulation protocols. Whole blood was collected from six healthy horses (aged 5-30 years; mixed breeds and genders) from the North Carolina State University teaching herd (IACUC #23-412). Sixty milliliters of heparinized blood were aseptically drawn and divided into 15 mL aliquots....
Journal of biomechanicsNovember 16, 2025
Volume 194 113078 doi: 10.1016/j.jbiomech.2025.113078
Opolz MD, Sipes GC, Moshage SG, McCoy AM, Kersh ME.Equine models are useful in biomechanics research due to their similarity in musculoskeletal tissue to humans, their athletic nature, and rapid skeletal development which permits ontogenetic studies. However, a continuing challenge in musculoskeletal models for large animal biomechanics is measuring the ground reaction force (GRF) during locomotion and therefore few reports of biomechanical measures such as joint torques. Here we evaluate two statistical approaches for estimating forelimb ground reaction forces in foals (n = 3). Longitudinal motion capture, GRF, and subject mass data during ...
Taned M, Zandi MB, Salek Ardestani S, Abdoli M, Spangler ML.Race performance in Thoroughbred horses is influenced by both genetic and environmental factors. Understanding their genetic architecture is essential for designing effective breeding programs. Objective: This study aimed to estimate genetic parameters and trends for key performance traits in Iranian Thoroughbred racehorses. Methods: Performance data, including race completion time (RCT), rank at the end of the competition (REC), and average speed (AS), were obtained from the Iranian Equestrian Federation (2002-2021). Pedigree data from 14,391 individuals were analyzed using CFC software. Gene...
Meng C, Wang J, Zeng Y, Yao X, Meng J.This study investigated the effects of varying proline supplementation doses in weaned foals. Twenty-eight weaned foals (approximately 5 months of age; body weight: 54.45 ± 11.33 kg; with an equal number of males and females) were randomly assigned to one of four groups-a control group, a low-dose group (20 mg/kg·d), a medium-dose group (40 mg/kg·d), and a high-dose group (60 mg/kg·d)- receiving continuous supplementation for 60 days. Blood samples were collected periodically for the analysis of hormones, antioxidants, immune parameters, and plasma amino acids. Concurrently, fecal 16S rRNA...
Góss GC, Mozzaquatro FD, Machado IRL, Gomes EM, Martins NR, Duarte CA.The collateral ligaments of the distal interphalangeal joint (CL-DIPJ) are important structures for athletic horses. Ligament injuries can cause lameness, but ultrasound imaging can detect such lesions, which usually present with changes in echogenicity and an increase in the cross-sectional area (CSA) of the ligament. Measurements of these structures vary among authors, which complicates the diagnosis. Thus, this study aimed to describe the ultrasound measurements (dorsopalmar diameter [DPD], lateromedial diameter [LMD], and CSA) of the CL-DIPJ in Argentine Polo horses. We measured the CL-DIP...
Lewczuk D, Borowska A, Andruszkiewicz J, Bagnicka E.Breeding value estimations of Warmblood horses in Poland are based on field performance, but the amount of stallions' data is still insufficient (334 horses). The first study's aim is to compare stallions' results in different stages and evaluate the overall ability using both stages. The effects on preselection (first stage free movement) and performance tests (second stage under rider) were analyzed separately and together as the overall ability. Spearman correlations were evaluated. The second aim was to evaluate the training period on the results. Due to the pandemic and lack of tests, hor...
Alsultan A, Karim SM, Al-Saadi M, Alsallami D, Ben Said M, Belkahia H.Equine piroplasmosis (EP), caused by the intracellular protozoa Theileria equi, Babesia caballi, and Theileria haneyi, represents a major health and economic threat to the equine industry worldwide. Existing diagnostic methods, including PCR, serology, and microscopy, are constrained by their dependence on specialized equipment, lengthy protocols, and the requirement for skilled personnel. Objective: This study aimed to develop a rapid, accurate, and field-deployable molecular diagnostic assay for T. equi. Methods: A nucleic acid-based diagnostic platform combining recombinase polymerase ampli...
Farias BJP, Furtado DA, Barbosa do Nascimento JW, Neto JPL, de Morais FTL, Santos TLS, Vasconcelos AS, Silva RC, Alves JIP, Mcmanus C, Silveira RMF....This study presents the development and proof of concept of a wearable monitoring system designed to measure respiratory rate (RR) and coat surface temperature (CST) in horses. The device integrates an embedded system based on an ESP-32 microcontroller equipped with sensors for RR (strain gauge) and CST (DS18B20) Data are transmitted in real time via Wi-Fi and stored locally on SD cards. The system was validated against conventional manual methods under field conditions. Agreement between methods was evaluated using Intraclass Correlation Coefficients (ICC) and Bland-Altman plots. Results show...
Broothaers K, Jouneau A, Angel-Velez D, De Coster T, Festuccia N, Archilla C, Calderari S, Jouneau L, Van den Branden E, Peere S, Polfliet E....In vitro production of equine embryos has been performed using intracytoplasmic sperm injection (ICSI) for the last two decades. Since 2022, a repeatable protocol for conventional in vitro fertilization (IVF) provides a successful alternative. However, little is known about the influence of the fertilization method on embryo quality and the transcriptomic profile. In this study, we aimed to examine differentially expressed genes (DEGs) between ICSI and IVF embryos in the horse. Therefore, ten equine sibling blastocysts, produced in vitro by either ICSI or IVF from three different mares, were s...
Woods T, Dixon J, Melvaine C, Fraser B.To determine the ideal location of a medial tenoscopic portal in the equine carpal flexor tendon sheath (CFTS) using contrast computed tomography (CT). Methods: Experimental cadaver study. Methods: A total of 20 entire forelimbs from adult Standardbred horses. Methods: Contrast media diluted in saline was injected into the CFTS from a lateral approach before manually flexing and extending the limb 20 times to disperse the contrast solution. The limbs were placed in lateral recumbency and a capped 21-gauge 1.5 inch needle was placed approximately 5 mm proximal and 32 mm caudal to the palpab...
Aarts RM, Smit IH, Ferraz GC, Rhodin M, Serra Braganca FM, Hernlund E.Assessment of equine metabolic and locomotory responses to quantified levels of physical exercise is needed to support the creation of tailored protocols for optimal training adaptation. The locomotory response to exercise in horses is not fully understood. Objective: To investigate the effect of a standardised exercise test (SET) on upper-body and limb kinematics and stride-to-stride variability in Standardbred Warmblood trotters. Methods: Experimental repeated-measures field study. Methods: Thirteen Standardbred trotters, equipped with nine inertial measurement units (IMUs), performed an inc...
Barlough JE, Madigan JE, DeRock E, Dumler JS, Bakken JS.A Thoroughbred filly that developed clinical signs of equine granulocytic ehrlichiosis following inoculation with the human granulocytotropic ehrlichia was shown to be resistant to challenge with Ehrlichia equi, a closely related agent. This result further substantiates the close and potentially conspecific relationship between these two granulocytotropic ehrlichiae.
Brama PA, Tekoppele JM, Bank RA, Barneveld A, van Weeren PR.Biochemical heterogeneity of cartilage within a joint is well known in mature individuals. It has recently been reported that heterogeneity for proteoglycan content and chondrocyte metabolism in sheep develops postnatally under the influence of loading. No data exist on the collagen network in general or on the specific situation in the horse. The objective of this study was to investigate the alterations in equine articular cartilage biochemistry that occur from birth up to age one year, testing the hypothesis that the molecular composition of equine cartilage matrix is uniform at birth and b...
Krueger K, Flauger B, Farmer K, Maros K.This study evaluates the horse (Equus caballus) use of human local enhancement cues and reaction to human attention when making feeding decisions. The superior performance of dogs in observing human states of attention suggests this ability evolved with domestication. However, some species show an improved ability to read human cues through socialization and training. We observed 60 horses approach a bucket with feed in a three-way object-choice task when confronted with (a) an unfamiliar or (b) a familiar person in 4 different situations: (1) squatting behind the bucket, facing the horse (2) ...
Wolff A, Hausberger M, Le Scolan N.Different tests were used to assess different aspects of the emotionality of 1-3 year-old horses: arena test; a `novel object' test; and a handling test. In reaction to the test situations no important differences were observed according to age or sex in the behaviour patterns, but clear individual differences were observed within these classes. The arena test seemed to reveal the degree of gregariousness of the animals whereas the results in the two other tests were correlated and seemed to reflect an inherent degree of fearfulness in the horse. Indices were developed that enabled to rank the...
Stanley RL, Fleck RA, Becker DL, Goodship AE, Ralphs JR, Patterson-Kane JC.Injury to the energy-storing superficial digital flexor tendon is common in equine athletes and is age-related. Tenocytes in the superficial digital flexor tendon of adult horses appear to have limited ability to respond adaptively to exercise or prevent the accumulation of strain-induced microdamage. It has been suggested that conditioning exercise should be introduced during the growth period, when tenocytes may be more responsive to increased quantities or intensities of mechanical strain. Tenocytes are linked into networks by gap junctions that allow coordination of synthetic activity and ...
Choi YH, Varner DD, Love CC, Hartman DL, Hinrichs K.Work with lyophilized sperm helps delineate the factors required for successful fertilization. We investigated the use of lyophilized sperm in equine embryo production. In Experiment 1, sperm DNA fragmentation index was not affected by three freeze/thaw or lyophilization cycles. In Experiment 2, oocytes injected with lyophilized sperm or with sperm from a treatment in which lyophilized sperm were suspended in sperm cytoplasmic extract (SE) yielded blastocyst development rates of 0 and 28% respectively (P < 0.05). In Experiment 3, blastocyst development rate was 6-11% after injection of sper...
Felkel S, Vogl C, Rigler D, Dobretsberger V, Chowdhary BP, Distl O, Fries R, Jagannathan V, Janečka JE, Leeb T, Lindgren G, McCue M, Metzger J....Analysis of the Y chromosome is the best-established way to reconstruct paternal family history in humans. Here, we applied fine-scaled Y-chromosomal haplotyping in horses with biallelic markers and demonstrate the potential of our approach to address the ancestry of sire lines. We de novo assembled a draft reference of the male-specific region of the Y chromosome from Illumina short reads and then screened 5.8 million basepairs for variants in 130 specimens from intensively selected and rural breeds and nine Przewalski's horses. Among domestic horses we confirmed the predominance of a young'c...
Santos AS, Rodrigues MA, Bessa RJ, Ferreira LM, Martin-Rosset W.Having evolved as a grazing animal, a horse's digestive physiology is characterized by rapid gastric transit, a rapid but intense enzymatic digestion along the small intestine, and a long and intense microbial fermentation in the large intestine. The process of understanding and describing feed degradation mechanisms in the equine digestive system in general, and in the hindgut ecosystem in particular, is essential. Regardless of its importance for the nutritional status of the host, the significance of the cecum-colon ecosystem has not yet been fully understood, and few reports have focused d...
Nakamura K, Takimoto-Inose A, Hasegawa T.Humans have domesticated many kinds of animals in their history. Dogs and horses have particularly close relationships with humans as cooperative partners. However, fewer scientific studies have been conducted on cognition in horses compared to dogs. Studies have shown that horses cross-modally distinguish human facial expressions and recognize familiar people, which suggests that they also cross-modally distinguish human emotions. In the present study, we used the expectancy violation method to investigate whether horses cross-modally perceive human emotions. Horses were shown a picture of a ...
Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T....Adipose tissue is a promising source of mesenchymal stromal cells (MSCs) for the treatment of tendon disease. The goal of this study was to assess the effect of a single intralesional implantation of adipose tissue-derived mesenchymal stromal cells (AT-MSCs) on artificial lesions in equine superficial digital flexor tendons (SDFTs). During this randomized, controlled, blinded experimental study, either autologous cultured AT-MSCs suspended in autologous inactivated serum (AT-MSC-serum) or autologous inactivated serum (serum) were injected intralesionally 2 weeks after surgical creation of cen...
Bowling AT, Del Valle A, Bowling M.Through DNA sequence comparisons of a mitochondrial D-loop hypervariable region, we investigated matrilineal diversity for Arabian horses in the United States. Sixty-two horses were tested. From published pedigrees they traced in the maternal line to 34 mares acquired primarily in the mid to late 19th century from nomadic Bedouin tribes. Compared with the reference sequence (GenBank X79547), these samples showed 27 haplotypes with altogether 31 base substitution sites within 397 bp of sequence. Based on examination of pedigrees from a random sampling of 200 horses in current studbooks of the A...
Mackie RI, Wilkins CA.Samples from the duodenum, jejunum, and ileum, as well as from the cecum and colon, were obtained from 11 mature grass-fed horses. Viable counts of total culturable and proteolytic bacteria were made on habitat-simulating media containing 40% clarified ruminal fluid. The mean pHs in the duodenum, jejunum, and ileum were 6.32, 7.10, and 7.47, respectively; the mean pH decreased to 6.7 in the hindgut. The acetate concentration increased along the length of the small intestine and was the only volatile fatty acid present in this gut segment. Molar proportions of acetate, propionate, and butyrate ...
Linklater WL.Socio-ecological explanations for intra- and interspecific variation in the social and spatial organization of animals predominate in the scientific literature. The socio-ecological model, developed first for the Bovidae and Cervidae, is commonly applied more widely to other groups including the Equidae. Intraspecific comparisons are particularly valuable because they allow the role of environment and demography on social and spatial organization to be understood while controlling for phylogeny or morphology which confound interspecific comparisons. Feral horse (Equus caballus Linnaeus 1758) p...
Wathan J, McComb K.Sensitivity to the attentional states of others has adaptive advantages, and in social animals, attending to others is important for predator detection, as well as a pre-requisite for normal social functioning and more complex socio-cognitive abilities. Despite widespread interest in how social species perceive attention in others, studies of non-human animals have been inconclusive about the detailed cues involved. Previous work has focused on head and eye direction, overlooking the fact that many mammals have obvious and mobile ears that could act as a visual cue to attention. Here we report...
Saeed MA, Beveridge I, Abbas G, Beasley A, Bauquier J, Wilkes E, Jacobson C, Hughes KJ, El-Hage C, O'Handley R, Hurley J, Cudmore L, Carrigan P....Equine gastrointestinal nematodes (GINs) have been the subject of intermittent studies in Australia over the past few decades. However, comprehensive information on the epidemiology of equine GINs, the efficacy of available anthelmintic drugs and the prevalence of anthelmintic resistance (AR) in Australasia is lacking. Herein, we have systematically reviewed existing knowledge on the horse GINs recorded in Australia, and main aspects of their pathogeneses, epidemiology, diagnoses, treatment and control. Methods: Six electronic databases were searched for publications on GINs of Australian hors...
Brandt L, Schubert S, Scheibe P, Brehm W, Franzen J, Gross C, Burk J.Transplantation of multipotent mesenchymal progenitor cells is a valuable option for treating tendon disease. Tenogenic differentiation leading to cell replacement and subsequent matrix modulation may contribute to the regenerative effects of these cells, but it is unclear whether this occurs in the inflammatory environment of acute tendon disease. Equine adipose-derived stromal cells (ASC) were cultured as monolayers or on decellularized tendon scaffolds in static or dynamic conditions, the latter represented by cyclic stretching. The impact of different inflammatory conditions, as represente...
Promerová M, Andersson LS, Juras R, Penedo MC, Reissmann M, Tozaki T, Bellone R, Dunner S, Hořín P, Imsland F, Imsland P, Mikko S, Modrý D....For centuries, domestic horses have represented an important means of transport and served as working and companion animals. Although their role in transportation is less important today, many horse breeds are still subject to intense selection based on their pattern of locomotion. A striking example of such a selected trait is the ability of a horse to perform additional gaits other than the common walk, trot and gallop. Those could be four-beat ambling gaits, which are particularly smooth and comfortable for the rider, or pace, used mainly in racing. Gaited horse breeds occur around the glob...
Leroux C, Craigo JK, Issel CJ, Montelaro RC.A primary mechanism of lentivirus persistence is the ability of these viruses to evolve in response to biological and immunological selective pressures with a remarkable array of genetic and antigenic variations that constitute a perpetual natural experiment in genetic engineering. A widely accepted paradigm of lentivirus evolution is that the rate of genetic variation is correlated directly with the levels of virus replication: the greater the viral replication, the more opportunities that exist for genetic modifications and selection of viral variants. To test this hypothesis directly, we ex...
Frisbie DD, Bowman SM, Colhoun HA, DiCarlo EF, Kawcak CE, McIlwraith CW.To evaluate a technique of autologous chondrocyte implantation (ACI) similar to the other techniques using cell-seeded resorbable collagen membranes in large articular defects. Methods: Autologous cartilage was harvested arthroscopically from the lateral trochlear ridge of the femur in fifteen 3-year-old horses. After culture and expansion of chondrocytes the newly created ACI construct (autologous chondrocytes cultured expanded, seeded on a collagen membrane, porcine small intestine submucosa) was implanted into 15mm defects on the medial trochlear ridge of the femur in the opposite femoropat...
Billich C, Sauder C, Frank R, Herzog S, Bechter K, Takahashi K, Peters H, Staeheli P, Schwemmle M.The recent observation that Borna disease virus (BDV)-reactive antibodies from psychiatric patients exhibit only low avidity for BDV antigen called into question their diagnostic value and raised the possibility that antigenically related microorganisms or self antigens caused the production of these antibodies. We further characterized the specificity of these antibodies. Methods: We established a peptide array-based screening test that allows the identification of antibodies directed against linear epitopes of the two major BDV proteins, the nucleoprotein (N) and the phosphoprotein (P). Resu...
Ricard A, Robert C, Blouin C, Baste F, Torquet G, Morgenthaler C, Rivière J, Mach N, Mata X, Schibler L, Barrey E.Endurance horses are able to run at more than 20 km/h for 160 km (in bouts of 30-40 km). This level of performance is based on intense aerobic metabolism, effective body heat dissipation and the ability to endure painful exercise. The known heritabilities of endurance performance and exercise-related physiological traits in Arabian horses suggest that adaptation to extreme endurance exercise is influenced by genetic factors. The objective of the present genome-wide association study (GWAS) was to identify single nucleotide polymorphisms (SNPs) related to endurance racing performance in 597 Ara...
Krueger K, Farmer K, Heinze J.Social learning is said to meet the demands of complex environments in which individuals compete over resources and cooperate to share resources. Horses (Equus caballus) were thought to lack social learning skills because they feed on homogenously distributed resources with few reasons for conflict. However, the horse's social environment is complex, which raises the possibility that its capacity for social transfer of feeding behaviour has been underestimated. We conducted a social learning experiment using 30 socially kept horses of different ages. Five horses, one from each group, were chos...
Brinkmann L, Gerken M, Riek A.Recent results suggest that the wild ancestor of the horse, the Przewalski horse, exhibits signs of a hypometabolism. However, there are speculations that domestic animals lost the ability to reduce energy expenditure during food shortage and adverse environmental conditions. Therefore, we investigated physiological and behavioural strategies employed by a robust domesticated horse breed, the Shetland pony, over the course of a year under temperate conditions by measuring ambient temperature (T(a)), subcutaneous temperature (T(s)), locomotor activity (LA), lying time, resting heart rate, body ...
Trösch M, Cuzol F, Parias C, Calandreau L, Nowak R, Lansade L.Over the last few years, an increasing number of studies have aimed to gain more insight into the field of animal emotions. In particular, it is of interest to determine whether animals can cross-modally categorize the emotions of others. For domestic animals that share a close relationship with humans, we might wonder whether this cross-modal recognition of emotions extends to humans, as well. In this study, we tested whether horses could recognize human emotions and attribute the emotional valence of visual (facial expression) and vocal (non-verbal vocalization) stimuli to the same perceptua...
Paterson YZ, Rash N, Garvican ER, Paillot R, Guest DJ.Autologous mesenchymal stem cells (MSCs) are an attractive concept in regenerative medicine, but their mechanism of action remains poorly defined. No immune response is reported after in vivo injection of allogeneic equine MSCs or embryo-derived stem cells (ESCs) into the equine tendon, which may be due to the cells' immune-privileged properties. This study further investigates these properties to determine their potential for clinical application in other tissues. Methods: Mitomycin C-treated MSCs, ESCs, or differentiated ESCs (dESCs) were cultured with allogeneic equine peripheral blood mono...
Smith RK, Gerard M, Dowling B, Dart AJ, Birch HL, Goodship AE.Over-strain injury of the superficial digital flexor tendon (SDFT) is a common injury in the horse. Tendon appears to adapt to loads placed on it during development, but fatigue damage accumulates after skeletal maturity, which is inadequately repaired and predisposes to clinical tendinitis. In any population of horses, there is a wide variation in SDFT mechanical properties. A noncollagenous protein, cartilage oligomeric matrix protein (COMP), is particularly abundant during growth in the equine SDFT and has been proposed to have an organisational role in the formation of collagenous matrices...
Kunz IGZ, Reed KJ, Metcalf JL, Hassel DM, Coleman RJ, Hess TM, Coleman SJ.The gastrointestinal microbiota (GIM) plays an essential role in maintaining intestinal homeostasis with disruptions having profound effects on the wellbeing of the host animal. Parasitic infection is a long-standing issue for the equine industry, and the use of anthelmintic drugs for parasite control has long been standard practice. The impact of anthelmintic treatment on the GIM in healthy horses is not well known. This study evaluated the hypothesis that anthelmintic administration will alter the equine fecal microbiota in horses without an observed helminth infection. Ten horses were treat...
Zerbe H, Schuberth HJ, Engelke F, Frank J, Klug E, Leibold W.In order to investigate pathogenic mechanisms of acute endometritis in cows and mares, we established an in vivo model in both species. Based on the results of an in vitro transmigration system, human recombinant interleukin-8 (rhIL-8; 1.25 microg per mare and 5 microg per cow in 50 ml phosphate-buffered saline) was used to attract polymorphonuclear neutrophil granulocytes (PMNs) into the uteri. Peak numbers of uterine neutrophils were attracted after 6h, in both cows and mares. On average, mares responded more sensitively than cows, with 15 times higher numbers of rhIL-8-attracted uterine neu...
Cheung WK, Working DM, Galuppo LD, Leach JK.Adipose stromal cells (ASC) are a promising alternative to progenitor cells from other tissue compartments because of their multipotential and capacity to retrieve significantly more progenitor cells. Initial cell samples are heterogeneous, containing a collection of cells that may contribute to tissue repair, but the sample becomes more homogeneous with each passage. Therefore, we hypothesized that the osteogenic potential of culture-expanded ASC would differ from uncultured ASC. Methods: Adipose tissue was collected from a yearling colt, and ASC were isolated and expanded using standard prot...
Kawcak CE, McIlwraith CW, Norrdin RW, Park RD, James SP.Subchondral bone plays a role in the pathogenesis of osteochondral damage and osteoarthritis in horses and humans. Osteochondral fragmentation and fracture, subchondral bone necrosis and osteoarthritis are common diseases in athletic horses, and subchondral bone is now thought to play an integral role in the pathogenesis of these diseases. There have been numerous research efforts focused on articular cartilage damage and its pathogenesis, yet comparatively little effort focused on subchondral bone pathology or the coordinated disease states of the osteochondral tissues. The purpose of this re...
