Analyze Diet

Topic:Grain

Grain is a common component of equine diets, often used to provide concentrated sources of energy and nutrients for horses. It is typically included in the form of oats, corn, barley, and other cereal grains. The inclusion of grain in a horse's diet can influence digestive health, metabolic function, and overall nutritional status. Understanding the effects of grain on equine health involves examining factors such as grain processing, feeding practices, and individual horse needs. This page compiles peer-reviewed research studies and scholarly articles that explore the impact of grain on equine nutrition, digestion, and metabolic health.
Effect of micronization and meal size of corn grain on glycemic response and in vitro hindgut acidosis potential in horses.
Journal of equine veterinary science    December 7, 2023   104982 doi: 10.1016/j.jevs.2023.104982
Varasteh F, Ebrahimi SH, Naserian AA, Zerehdaran S, Miri VH.This study aimed to 1) evaluate the interaction of corn grain micronization and starch levels per meal on equine plasma glucose, and 2) determine if micronization affects the risk of hindgut acidosis. Six mature (aged 6 to 10 years), healthy, non-pregnant mares (initial body weight [BW]: 301 to 463 kg) were used in a 2×3 factorial cross-over design. The treatments included two forms of corn grain (ground and micronized flaked) at three levels of starch (1, 1.5, and 2 g/kg BW per meal). The blood was sampled before and 30, 60, 90, 120, 180, 240, and 300 min after morning feeding and the glucos...
Influence of feeding and other factors on adrenocorticotropin concentration and thyrotropin-releasing hormone stimulation test in horses and ponies.
Equine veterinary journal    November 27, 2023   doi: 10.1111/evj.14030
Drozdzewska K, Winter J, Barton AK, Merle R, Gehlen H.The basal (bACTH) and post-thyrotropin-releasing hormone stimulation concentration of adrenocorticotropin (pACTH) are recommended for diagnosis of pituitary pars intermedia dysfunction (PPID). Many factors influence bACTH (e.g., disease, age, month) and some affect the results only in autumn (e.g., breed, colour, sex). There are discrepancies about the impact of feeding on b/pACTH. Objective: To determine whether feeding, month, age, breed, colour, sex and body condition score affect b/pACTH. Methods: Prospective crossover. Methods: Sixty-one animals were divided into groups: healthy, PPID, tr...
Effects of different grains on bacterial diversity and enzyme activity associated with digestion of starch in the foal stomach.
BMC veterinary research    November 17, 2022   Volume 18, Issue 1 407 doi: 10.1186/s12917-022-03510-2
Li XB, Huang XX, Li Q, Li XY, Li JH, Li C, He LJ, Jing HX, Yang KL.Compared with the stomach of ruminant cattle, the stomach of horse is small and mainly for chemical digestion, but the microorganisms in the stomach play an important role in maintaining the homeostasis of the internal environment. Due to the complexity of the microbes in the stomach, little is known about the diversity and structure of bacteria in the equine stomach. Grains are the main energy source for plant-eating livestock and energy is derived through enzymatic hydrolysis of grains into glucose or their microbial fermentation into Volatile fatty acids (VFA). However, the mechanism throug...
Reinforcer efficacy of grain for horses.
Journal of the experimental analysis of behavior    September 2, 2022   Volume 118, Issue 2 302-315 doi: 10.1002/jeab.797
Platzer J, Feuerbacher EN.Positive reinforcement is becoming more common in horse training. Identifying effective reinforcers is critical for training success. The aim of this study was to determine relative reinforcer efficacy of different grains. Four horses learned to muzzle touch a target, after which they were tested using a progressive ratio schedule of reinforcement with different grains as the consequence. Break points were used to determine relative reinforcer efficacy of each grain and were also converted into unit price per kilocalorie to determine if caloric value impacted reinforcer efficacy. Condition 1 c...
Caecal fermentation characteristics of commonly used feed ingredients.
Equine veterinary journal    December 8, 2020   Volume 53, Issue 5 1056-1062 doi: 10.1111/evj.13390
Ochonski P, Drouillard JS, Douthit TL, Vahl C, Lattimer JM.Commercial horse feeds use cereal grains and by-products; however, their effects on the caecal environment remain poorly characterised. Objective: Characterise the effect of commonly used feed ingredients on caecal pH and volatile fatty acid (VFA) concentration. Methods: 6 × 6 Latin square. Methods: Two days prior to the start of the study, 6 caecally cannulated Quarter horses were moved into individual stalls where Smooth bromegrass hay (brome) was offered at 2.0% BW/d split between 2 feedings (0600 and 1800). On day 0, caecal digesta was collected every 2 h for 12 h relative to the 0600...
Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.
Journal of applied microbiology    June 7, 2019   Volume 127, Issue 2 344-353 doi: 10.1111/jam.14251
Shakarami MH, Mohammadabadi T, Motamedi H, Sari M, Teimouri Yansari A.This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS). Results: Fresh faeces were collected from four Arabian horses. The cellulose-hydrolytic bacteria were isolated by using a medium amended with carboxymethyl cellulose (CMC). The activity of CMC was determined by measuring the release of reducing sugars from CMC. Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity. 16S rRNA sequence analysis showed that these isolat...
In Vitro Digestibility Measurement of Feedstuffs in Donkeys Using the DaisyII Incubator.
Journal of equine veterinary science    February 14, 2019   Volume 75 122-126 doi: 10.1016/j.jevs.2019.02.002
Tassone S, Renna M, Barbera S, Valle E, Fortina R.Successful studies on in vitro digestibility measurement of feedstuffs with fecal inoculum have been reported for horses, whereas data on donkeys are currently lacking. In this study, we evaluated the use of the Daisy Incubator for in vitro digestibility measurement of feedstuffs using donkey feces as source of microbial inoculum. The method was tested using seven feedstuffs commonly used in donkey diets (alfalfa, bromegrass, ryegrass, and timothy hays; wheat bran and wheat straw; barley grains). Feces were obtained from four female donkeys, and incubations were carried out at 1-week interva...
Risks for animal health related to the presence of zearalenone and its modified forms in feed.
EFSA journal. European Food Safety Authority    July 31, 2017   Volume 15, Issue 7 e04851 doi: 10.2903/j.efsa.2017.4851
Knutsen HK, Alexander J, Barregård L, Bignami M, Brüschweiler B, Ceccatelli S, Cottrill B, Dinovi M, Edler L, Grasl-Kraupp B, Hogstrand C....Zearalenone (ZEN), a mycotoxin primarily produced by Fusarium fungi, occurs predominantly in cereal grains. The European Commission asked EFSA for a scientific opinion on the risk to animal health related to ZEN and its modified forms in feed. Modified forms of ZEN occurring in feed include phase I metabolites α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), α-zearalanol (α-ZAL), β-zearalanol (β-ZAL), zearalanone (ZAN) and phase II conjugates. ZEN has oestrogenic activity and the oestrogenic activity of the modified forms of ZEN differs considerably. For ZEN, the EFSA Panel on Contaminants...
Exogenous lactobacilli mitigate microbial changes associated with grain fermentation (corn, oats, and wheat) by equine fecal microflora ex vivo.
PloS one    March 30, 2017   Volume 12, Issue 3 e0174059 doi: 10.1371/journal.pone.0174059
Harlow BE, Lawrence LM, Harris PA, Aiken GE, Flythe MD.Cereal grains are often included in equine diets. When starch intake exceeds foregut digestion starch will reach the hindgut, impacting microbial ecology. Probiotics (e.g., lactobacilli) are reported to mitigate GI dysbioses in other species. This study was conducted to determine the effect of exogenous lactobacilli on pH and the growth of amylolytic and lactate-utilizing bacteria. Feces were collected from 3 mature geldings fed grass hay with access to pasture. Fecal microbes were harvested by differential centrifugation, washed, and re-suspended in anaerobic media containing ground corn, whe...
Effect of starch source (corn, oats or wheat) and concentration on fermentation by equine faecal microbiota in vitro.
Journal of applied microbiology    September 28, 2015   Volume 119, Issue 5 1234-1244 doi: 10.1111/jam.12927
Harlow BE, Donley TM, Lawrence LM, Flythe MD.The goal was to determine the effect of starch source (corn, oats and wheat) and concentration on: (i) total amylolytic bacteria, Group D Gram-positive cocci (GPC), lactobacilli and lactate-utilizing bacteria, and (ii) fermentation by equine microbiota. Results: When faecal washed cell suspensions were incubated with any substrate amylolytics increased over time. However, at 24 h there were 10 and 1000-fold more amylolytics with corn than wheat or oats respectively. Predominant amylolytics isolated were Enterococcus faecalis (corn, wheat) and Streptococcus bovis (oats). GPC increased with any ...
Effects of Saccharomyces cerevisiae supplementation on apparent total tract digestibility of nutrients and fermentation profile in healthy horses.
Journal of animal physiology and animal nutrition    May 10, 2013   Volume 97 Suppl 1 115-120 doi: 10.1111/jpn.12043
Mackenthun E, Coenen M, Vervuert I.Supplementation with Saccharomyces cerevisiae (SC) in horses may have some potential to modify microbial populations and thereby improve fibre digestibility. The aim of this study was to investigate the effects of SC on apparent total tract digestibility (ATTD) of nutrients, with a special focus on fibre digestion in healthy horses. The fermentation profile of microbial populations was another focus of interest. Twelve geldings were randomly assigned to three groups. The basal diet consisted of cracked corn (2 g starch/kg body weight [BW]) and hay (1.2 kg/100 kg BW). During adaptation (3 weeks...
Apparent digestibility of wheat bran and extruded flax in horses determined from the total collection of feces and acid-insoluble ash as an internal marker.
Animal : an international journal of animal bioscience    March 23, 2012   Volume 6, Issue 2 227-231 doi: 10.1017/S1751731111001297
De Marco M, Miraglia N, Peiretti PG, Bergero D.Several studies have reported data on comparisons between two methods: the total collection of feces and the internal markers method. The aim of this study was to assess the apparent digestibility of two concentrates and to compare the apparent digestion coefficients using the total collection of feces and acid-insoluble ash (AIA) as the internal marker method. In 2009, six adult geldings aged between 3 and 11 years, with an average weight per trial of 543, 540 and 542 kg, respectively, were used to determine the apparent digestibility by means of three in vivo digestibility trials on hay, hay...
Effect of protein source on nitrogen balance and plasma amino acids in exercising horses.
Journal of animal science    November 12, 2010   Volume 89, Issue 3 729-735 doi: 10.2527/jas.2010-3081
Graham-Thiers PM, Bowen LK.Plasma AA in horses fed either an all-hay or a hay and grain diet in a traditional format have not been investigated. Eight horses were divided into 2 groups: a hay group fed only grass hay or a hay and a grain group (HG) fed in a crossover design for two 5-wk periods. After the first period, horses were fasted overnight, followed by feeding with blood sampling every hour for 6 h. A 4-d total fecal and urine collection to evaluate N balance followed. A 10-d washout period separated the 5-wk feeding periods, during which horses switched diets. The second period was also followed by fasting, fee...
Effects of fusariotoxins in the equine species.
Veterinary journal (London, England : 1997)    October 17, 2009   Volume 186, Issue 2 157-161 doi: 10.1016/j.tvjl.2009.09.020
Caloni F, Cortinovis C.Fumonisins, trichothecenes and zearalenone are the most commonly occurring Fusarium mycotoxins in cereal grains and animal feed. In this review, the toxicity of these mycotoxins in horses is considered with particular reference to recent data on specific and proposed syndromes. Compared to other animal species, very little information is available on the adverse effects of fusariotoxins in horses. Fumonisin B(1) (FB(1)) is the causative agent of leukoencephalomalacia, which is typified by depression, aimless circling, head pressing, paresis, ataxia, blindness and death. FB(1) has also been sho...
Effect of type of grain and feed processing on gastrointestinal retention times in horses.
Journal of animal science    August 14, 2009   Volume 87, Issue 12 3991-3996 doi: 10.2527/jas.2008-1150
Rosenfeld I, Austbø D.Gastrointestinal retention time may affect digestive processes in the horse. To evaluate the effect of processing of grains on mean retention time in different segments of the gastrointestinal tract, 4 Norwegian Cold-blooded trotters (cecally cannulated, approximately 500 kg of BW) were used. Barley, maize, and wheat were all ground, pelleted, extruded, and micronized to create a total of 12 processed grains. After an adaptation period of 5 d, each horse was given 0.2 kg of Yb-mordanted grain together with their morning meal, which consisted of 2 kg of hay and 1 kg of one of the grains. Fecal ...
Effect of live yeast culture supplementation on apparent digestibility and rate of passage in horses fed a high-fiber or high-starch diet.
Journal of animal science    October 2, 2007   Volume 86, Issue 2 339-347 doi: 10.2527/jas.2006-796
Jouany JP, Gobert J, Medina B, Bertin G, Julliand V.Eight crossbred male horses aged 12 +/- 5 yr and with BW of 305 +/- 18 kg were used in pairs in a 4 x 4 Latin square design with 4 ground and pelleted diets. Each pair included a cecum and right ventral colon-fistulated animal and a cecal-fistulated animal. The 4 horse diets were a high-fiber diet (HF+0) based on dehydrated alfalfa, a high-starch diet based on barley and wheat bran (HS+0), and the HF or HS diets supplemented with Saccharomyces cerevisiae (SC) CBS 493.94 (HF+SC and HS+SC). The probiotic preparation contained 4.5 x 10(9) cfu/g of live yeast mixed with the culture medium, and was...
The effect of current grain feeding practices on hindgut starch fermentation and acidosis in the Australian racing Thoroughbred.
Australian veterinary journal    November 10, 2006   Volume 84, Issue 11 402-407 doi: 10.1111/j.1751-0813.2006.00059.x
Richards N, Hinch G, Rowe J.A survey of 72 thoroughbred trainers in several regions of NSW was conducted to better define current grain feeding practices, and the risks involved with grain feeding in the Australian thoroughbred racing industry. Results from the survey indicate that horses in the industry are currently being fed an average of 7.3 +/- 0.23 kg of grain concentrate per day, with oats, commercial pre-mixed diets and corn most commonly used. The feeding of extruded, micronised and steam flaked grains was uncommon. The results of grain and faecal sample analyses conducted during the survey indicate that incompl...
[Animal nutrition for veterinarians–case study: colic in ponies in a “petting zoo” caused by ingestion of higher amounts of wheat (Triticum aestivum L.)].
DTW. Deutsche tierarztliche Wochenschrift    November 3, 2006   Volume 113, Issue 10 385-387 
Wolf P, Kamphues J.Nutritional disorders in domestic or pet animals depend not seldom on special situations, for example when different influences coincide concerning keeping, housing and offering of feed or water. These lead suddenly to high-risk situations, that can cause sometimes the death of the animal. The following case report deals with two ponies, that were kept on a so-called "petting zoo" and showed repeatedly colic symptoms (always after a weekend). During surgery in the stomach light yellow balls were found, that can be explained by ingestion of 1.0 to 1.5 kg wheat. This cereals consist--depending o...
Effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on feed intake, serum chemistry, and hematology of horses, and the efficacy of a polymeric glucomannan mycotoxin adsorbent.
Journal of animal science    September 13, 2003   Volume 81, Issue 9 2123-2130 doi: 10.2527/2003.8192123x
Raymond SL, Smith TK, Swamy HV.The feeding of Fusarium mycotoxin-contaminated grains adversely affects the performance of swine and poultry. Very little information is available, however, on adverse effects associated with feeding these mycotoxin-contaminated grains on the performance of horses. An experiment was conducted to investigate the effects of feeding a blend of grains naturally contaminated with Fusarium mycotoxins on feed intake, serum immunoglobulin (Ig) concentrations, serum chemistry, and hematology of horses. A polymeric glucomannan mycotoxin adsorbent (GM polymer) was also tested for efficacy in preventing F...
Mycotoxins.
The Veterinary clinics of North America. Equine practice    January 10, 2002   Volume 17, Issue 3 547-viii doi: 10.1016/s0749-0739(17)30051-2
Osweiler GD.Horses consume feed grains and forages that can produce a range of mycotoxins resulting from mold invasion. Toxicosis of horses often occurs from fumonisins or aflatoxin in grains, from the tremorgenic mycotoxins in dallis grass, or from slaframine in red clover. Fumonisin toxicosis often is severe and fatal, and aflatoxin can be acute or chronic and debilitating. Other mycotoxins reported in horses may cause moderate to mild signs that regress when the contaminated feedstuff is removed. Overall, horses appear to have a relatively low prevalence of reported mycotoxicoses among domestic animals...
Role of duodenal reflux in nonglandular gastric ulcer disease of the mature horse.
Equine veterinary journal. Supplement    March 4, 2000   Issue 29 24-29 doi: 10.1111/j.2042-3306.1999.tb05164.x
Berschneider HM, Blikslager AT, Roberts MC.Gastric contents were sampled in horses via nasogastric tube to determine changes in pH and bile salt concentrations during feeding and fasting periods. The horses were rotated through 4 feeding protocols. (1) hay; (2) hay with twice daily grain meals; (3) and (4) fasting preceded by either hay only or hay and grain. Sequential, hourly samples were collected from 3 horses prepared with gastric cannulas. Horses were fed hay twice daily and grain mix either twice daily or in small aliquots dispensed every 90 min. The horses were sampled during normal feeding or after 14 h of feed deprivation. Ga...
The effects of timing and amount of forage and grain on exercise response in thoroughbred horses.
Equine veterinary journal. Supplement    February 5, 2000   Issue 30 451-457 doi: 10.1111/j.2042-3306.1999.tb05264.x
Pagan JD, Harris PA.There is considerable debate among horsemen about how to feed horses before exercise. Should horses be fed or fasted before work and when should hay be fed relative to grain and/or exercise? Three experiments were conducted to evaluate if feeding hay with and without grain affects glycaemic and haematological responses in Thoroughbred (TB) horses at rest and during a simulated competition exercise test (CET) on a high-speed treadmill. In Experiment 1, 6 TB horses were fed hay at 3 different times relative to a grain meal. Time of feeding hay affected glycaemic response, plasma protein and wate...
Effects of differently composed feeds and physical stress on plasma gastrin concentration in horses.
Acta veterinaria Scandinavica    October 27, 1998   Volume 39, Issue 2 265-272 doi: 10.1186/BF03547798
Sandin A, Girma K, Sjöholm B, Lindholm A, Nilsson G.Plasma gastrin concentrations were determined in 6 Standardbreds (4 geldings and 2 mares) after 3 different meals consisting of unlimited amounts of hay (8-9 kg per horse), a restricted amount of hay (0.6 kg/100 kg body-weight) and grain (0.2 kg/100 kg body-weight) in combination or of grain alone (0.2 kg/100 kg body-weight). In another series of experiments the possible role of gastrin as a stress hormone was investigated. Plasma gastrin and cortisol concentrations were determined during fasting and compared with concentrations during hay feeding. In addition, gastrin and cortisol concentrati...
Environmental effects on thermoregulation and nutrition of horses.
The Veterinary clinics of North America. Equine practice    August 1, 1990   Volume 6, Issue 2 355-372 doi: 10.1016/s0749-0739(17)30546-1
Cymbaluk NF, Christison GI.Horses are reared in all types of weather. Temperatures as diverse as -40 degrees C to 40 degrees C are tolerated by horses. The nutrient requirement most influenced by cold weather is energy. In cold weather, feeding good quality hays free-choice is usually sufficient for mature horses in good body condition. Grain may have to be fed when poor quality hays are used. Hot weather (greater than 30 degrees C) necessitates heat loss to maintain body core temperature. Horses sweat to reduce body heat. Heat stress can be minimized by feeding diets that reduce the heat increment. Use of grain and fat...
Fusariotoxicoses of farm animals and mycotoxic leucoencephalomalacia of the equine associated with the finding of trichothecenes in feedstuffs.
Veterinary and human toxicology    June 1, 1986   Volume 28, Issue 3 207-212 
Gabal MA, Awad YL, Morcos MB, Barakat AM, Malik G.Mycotoxicoses involving horses, rabbits and cattle have been studied. Fusarium tricinctum and T-2 toxin were isolated from all incriminated feedstuffs. Other isolated trichothecenes from the feed included HT-2, verrucarins and roridin. The toxins were separated, identified and quantitated using thin-layer chromatography, gas-liquid chromatography and mass spectrometry. T-2 toxin was detected in amounts varying from 0.5-204 ppm. The contaminated feedstuffs in connection with the affected horses and rabbits, included cereal grains (corn, cornstalks, bran and barley), whereas fescue hay was assoc...
Feed preferences of ponies.
Equine veterinary journal    January 1, 1985   Volume 17, Issue 1 20-22 doi: 10.1111/j.2042-3306.1985.tb02031.x
Hawkes J, Hedges M, Daniluk P, Hintz HF, Schryver HF.Preference trials were conducted with mature ponies. In Trial 1, oats were compared with oats plus sucrose. Four of six pony geldings selected oats plus sucrose, but one pony demonstrated a dislike for sucrose and one selected from the bucket on the right side regardless of content. Oats, maize, barley, rye and wheat were compared in Trial 2 using six mature pony mares. Oats were the preferred grain, with maize and barley ranking second and third respectively. Wheat and rye were the least preferred. Even though the ponies demonstrated preference, the total intake at a given meal was not greatl...
Some nutritional aspects of colic in horses.
Modern veterinary practice    May 1, 1984   Volume 65, Issue 5 A9-A12 
Hintz HF.Consistency of exercise and diet are important in colic prevention. Water should be offered before and after feeding. Fast-growing foals suckling heavily lactating mares may overeat grain at weaning. Creep feeding to accustom the foal to eating grain and gradually increasing the grain intake after weaning are helpful in preventing colic in foals. Stallions may overeat grain when taken off pasture in hot weather. Feeding hay initially and grain later helps avoid colic in these stallions. Type-D Clostridium perfringens may cause enterotoxemia in foals. Corn should be fed in moderation. High-Mg d...
Mouldy grain and the aetiology of pellagra: the role of toxic metabolites of Fusarium.
Biochemical Society transactions    February 1, 1980   Volume 8, Issue 1 147-150 doi: 10.1042/bst0080147
Schoental R.No abstract available
Digestion coefficients, blood glucose levels and molar percentage of volatile acids in intestinal fluid of ponies fed varying forage-grain ratios.
Journal of animal science    November 1, 1971   Volume 33, Issue 5 992-995 doi: 10.2527/jas1971.335992x
Hintz HF, Argenzio RA, Schryver HF.No abstract available