"Horses" is a broad topic that encompasses various aspects of equine biology, behavior, and management. This category includes studies on the anatomy, physiology, and genetics of horses, as well as their behavior, nutrition, and care. Research in this area may also cover the historical and cultural significance of horses, their roles in agriculture, sport, and therapy, and the challenges associated with their conservation and welfare. The page aggregates peer-reviewed research articles and scholarly studies that explore the multifaceted relationships between humans and horses, examining both scientific and socio-economic perspectives.
Cope SE, Catts EP.Parahost behavior of adult Gasterophilus intestinalis (DeGeer) was observed during the summers of 1978 and 1979 near pastured horses on two farms near Newark, Del. Adult fly occurrence, although sparse in early summer, indicated a gradual increase, with three surges in emergence. In the laboratory, males lived 1-3 d and females 1-2 d. In the field, marked flies were not seen on days following their release. Adult behavior indicated an urgency in mating and oviposition dictated by a short life span. Male flight patterns were characterized by in-flight searches for females and intraspecific conf...
Hunt RJ, Allen D, Baxter GM, Jackman BR, Parks AH.Deep digital flexor tendon transection at the mid-metacarpus was performed in 20 horses with severe acute or chronic laminitis that was not responsive to conventional treatment. Sixteen horses improved within 72 hours, one horse worsened, and two horses were unaffected by the surgery. Eleven horses survived less than 1 month after surgery and six horses survived longer than 6 months. Three horses surviving longer than 6 months have remained lame and no horse has returned to athletic performance. Transection of the deep digital flexor tendon at the mid-metacarpus may decrease the pain associate...
Lees MJ, Fretz PB, Bowen CV, Leach DH.Equine limb wounds often heal slowly by epithelialization, and large scars are a frequent end result. In some ways, they resemble the wound associated with human tibial injuries. The literature indicates that previous investigators have failed to transfer free skin flaps successfully in the horse. In this paper, we review our experimental work with the deep circumflex iliac flap in the horse. Dissections of 20 cadavers confirmed the anatomical consistency of the flap. Four flaps survived well when elevated as island flaps, but five orthotopic and nine heterotopic free flap transfers all failed...
al-Bagdadi F, Hoyt P, Karns P, Martin G, Memon M, McClure R, McCoy D, Shoemaker S.Eleven unilateral cryptorchid stallions, two to three years old, were castrated at Louisiana State University Veterinary Teaching Hospital. Five of these cryptorchid cases were abdominal and the rest were inguinal. This study was initiated to document the differences between the abdominal and inguinal equine cryptorchid testes. Specimens were obtained from the abepididymal side of each cryptorchid testes and processed for light and electron microscopic study. The cryptorchid testes were smaller than the scrotal testes, with the abdominal testes being one-fourth the size of the scrotal testes. ...
Flisińska-Bojanowska A, Gill J, Komosa M.1. The diurnal changes in the levels of lactic (LA) and pyruvic (PA) acids and values of pH were studied in standard-bred mares and their foals, for 13 weeks of foal life, throughout 2 years. 2. The studies began when a foal was 7 days old and were repeated every 2 weeks until foals reached 13 weeks of age. 3. Blood samples were taken every 4 hr for one day, each second week. 4. In the LA, PA levels and pH values no diurnal rhythm was stated in lactating mares during study periods. 5. In foals the diurnal rhythm in LA and PA occurred in the 3rd month of their life, with the acrophase at night ...
Bagy MM, Abdel-Mallek AY.Hair samples from different animals collected from different sites in Riyadh, Saudi Arabia were examined for the presence of saprophytic and keratinolytic fungi. Fifty-one specimens were examined of which 19 were from rabbits, 13 from sheeps, 12 from camels and 7 from horses. Forty-eight species and one variety which belong to 24 genera were collected during this investigation. The most common genera were Aspergillus and Chrysosporium. In low frequency, members of the genera Thermoascus, Scopulariopsis, Alternaria, Penicillium, Rhizopus, Botryotrichum, Fusarium, Ulocladium and keratinophilic f...
Hermanson JW, Hegemann-Monachelli MT, Daaod MJ, LaFramboise WA.The biceps brachii of horses is subdivided into a lateral and medial head. Electrophoresis of samples from the lateral head revealed three slow-migrating native myosin isoforms, including one that does not correspond to slow myosin isoforms described for other mammalian muscles. In contrast, the medial head contained a single slow isoform. Both the lateral and medial heads contained three fast-migrating isoforms corresponding with the FM-2, FM-3 and FM-4 isoforms reported for other mammalian fast-twitch muscle fibers. Electrophoresis of myosin heavy chains (MHCs) revealed only two MHC bands, o...
Baker CB, Adams MH, McDowell KJ.Horse conceptuses were collected on Days 13, 15, 20 and 25 after ovulation. Whole conceptuses (Days 13 and 15) or extra-embryonic membranes (Days 20 and 25) were homogenized and poly-adenylated RNA (poly A RNA) was isolated by binding to oligo (dT)-cellulose. Poly A RNA (1 microgram/well) was separated by size on a denaturing 1% agarose gel and blotted onto nitrocellulose filters (northern blotting). DNA probes were prepared from plasmids containing equine alpha 1, omega 1 and omega 2 interferons and human beta actin. The presence of messenger RNA (mRNA) was detected by specific hybridization ...
Bowling AT, Williams MJ.Two additional specificities (Dq and Dr) were assigned to the D system of horse red cell alloantigens following discussion of the 1989 ISAG Horse Comparison Test (HCT) results. Family and population data support 25 phenogroups defined by the enhanced battery of 17 D system factors.
Prescott JF.Recent isolations of Rhodococcus equi from cavitatory pulmonary disease in patients with AIDS have aroused interest among medical microbiologists in this unusual organism. Earlier isolations from humans had also been in immunosuppressed patients following hemolymphatic tumors or renal transplantation. This organism has been recognized for many years as a cause of a serious pyogranulomatous pneumonia of young foals and is occasionally isolated from granulomatous lesions in several other species, in some cases following immunosuppression. The last decade has seen many advances in understanding o...
Orsini JA, Dreyfuss DJ, Vecchione J, Spencer PA, Uhlman R.The effects of a potent new histamine-2 (H2) receptor antagonist, BMY-25368, were studied on gastric acid secretion in 5 foals from which food was withheld. Doses of 0.02, 0.11, 0.22, and 1.10 mg/kg of body weight were administered IM in a randomly assigned treatment sequence. Following BMY-25368 administration, hydrogen ion concentration was decreased and mean pH was higher than baseline values in a dose-response pattern. At the 0.22 and 1.10 mg/kg doses, the high pH was sustained for greater than 4 hours. The BMY-25368 thus may be useful for treating gastric ulcer disease in horses.
Pellegrini A, Waiblinger S, Von Fellenberg R.Lysozyme from equine neutrophil granulocytes was isolated in a pure form by fast performance liquid chromatography, i.e. ion-exchange chromatography and reversed-phase chromatography. The lysozyme lysed Micrococcus luteus, Bacillus subtilis and Staphylococcus lentus and was also bactericidal against the Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae, Bordetella bronchiseptica, and Serratia marcescens. Staphylococcus aureus and Staphylococcus epidermidis were not lysed. The lysozyme was only very slightly bactericidal for S. epidermidis and S. aureus. Equine neutrophil lysozyme ...
Cleve H, Schmid DO.An apparent incompatibility in the GC/DBP system in a mare and her colt was found after classification by polyacrylamide gel electrophoresis. Because of this observation an analysis of the equine GC/DBP system by isoelectric focusing and subsequent immunoprinting was initiated. Further GC subtypes, however, were not encountered: the three phenotypes GC F, FS and S were also delineated by this method. The GC types in the case of disputed descent were dissolved: GC S was found in the mare and GC FS in her colt as well as in another of her male offspring. It is proposed that a rare GC mutant is r...
Ledwozyw A, Kadziołka A, Jabłonka S, Tusińska E, Herbut M.The aim of this study was to investigate the platelet factor 3 activity in platelet-rich plasma of horse, cattle, sheep and pig, by the use of chromogenic tripeptide substrate H-D-Phenylalanyl-Pipecolyl-Arginyl-p-nitroanilide. Among species examined the highest activity of this factor was stated in pig, the lowest one in sheep. Chromogenic substrate test was 10 times more sensitive that Stypven clotting time test. Thus, the use of chromogenic tripeptide substrate is fully valuable in platelet factor 3 activity estimation in domestic animals.
Cothran EG, Henney PJ, King JA.The inheritance of the equine Tf F3 allele was examined in 39 parent-offspring combinations. For 26 of the cases the allele inherited by the offspring from the heterozygous parent could be determined. The proportion of individuals that inherited the F3 variant compared to the alternative allele was exactly 1:1. In five cases the parental phenotype was identical to that of the offspring. For the remaining eight cases the parent was homozygous for the F3 allele and all offspring had the F3 allele. The results were consistent with Mendelian inheritance.
Vallejo AN, Miller NW, Clem LW.This work was undertaken to investigate whether or not antigen processing and presentation are important in channel catfish in vitro secondary immune responses elicited with structurally defined proteins, namely, pigeon heart cytochrome C (pCytC), hen egg lysozyme, and horse myoglobin. The use of in vitro antigen-pulsed and fixed B cells or monocytes as antigen presenting cells (APC) resulted in autologous peripheral blood leukocytes (PBL) responding with vigorous proliferation and antibody production in vitro. In addition, several long-term catfish monocyte lines have been found to function a...
O'Rourke KI, Besola ML, McGuire TC.Proviral sequences in the peripheral blood mononuclear cells of 3 horses with acute equine infectious anemia virus were monitored using the polymerase chain reaction. Provirus was detected during the initial viremic episode in each horse and during each of 3 relapsing viremic cycles, although the appearance of provirus lagged behind the onset of viremia. Following each viremic episode, provirus levels in the peripheral monocytes decreased to less than 1 copy in 5 x 10(6) cells.
Eysker M, Boersema JH, Kooyman FN.Two groups of three ponies were used to study the effect of three ivermectin or pyrantel treatments given at intervals of 5 weeks at the beginning of the grazing season. Although each pyrantel treatment resulted in a greater than 95% reduction in faecal egg counts during the first 3 weeks, high pasture larval counts were seen from the beginning of August onwards and substantial cyathostomine burdens were found at necropsy in December. The ivermectin treatments resulted in an even more pronounced reduction in faecal egg output, and the pasture larval counts and cyathostomine burdens at necropsy...
Angelos JA, Marti E, Lazary S, Carmichael LE.The DNA from equine sarcoid samples from New York State and Switzerland was isolated and probed with bovine papillomavirus type 1 (BPV-1) to determine if BPV genomes were present. Twelve of 13 sarcoids from New York State and 17/20 sarcoids from Switzerland contained DNA that hybridized to the BPV-1 probe. Restriction enzyme analysis of the positive samples demonstrated restriction fragment profiles characteristic of BPV-1 in 22 sarcoids and restriction fragment profiles characteristic of bovine papillomavirus type 2 (BPV-2) in 7 sarcoids. In addition, three tissues histologically diagnosed as...
Brooks M, Leith GS, Allen AK, Woods PR, Benson RE, Dodds WJ.Bleeding diathesis in a Quarter Horse filly was caused by von Willebrand disease. Hemorrhage occurred mainly from mucosal surfaces and after trauma. Quantitative and qualitative measurements of plasma von Willebrand factor (vWF) documented a specific deficiency of vWF high molecular weight multimers, and concurrently greater than expected deficiency of vWF activity relative to vWF concentration. These findings are characteristic of type-II von Willebrand disease in human beings. Application of vWF assays used in human and small animal medicine now permits evaluation of vWF and diagnosis of von...
Clark ES, Gantley B, Moore JN.The effects of intravenous (iv) infusion of endotoxin for 60 mins at a cumulative dosage of 0.03 micrograms/kg bodyweight on systemic arterial, right atrial and pulmonary arterial pressures, heart rate, cardiac output, and derived pulmonary vascular resistance and total peripheral vascular resistance were compared to the effects of iv infusion of saline solution in four healthy horses. Heart rate was increased significantly after endotoxin infusion, although diastolic arterial pressure, systolic arterial pressure, electronically averaged arterial pressure, cardiac output, total peripheral resi...
Broadstone RV, LeBlanc PH, Derksen FJ, Robinson NE.The in vitro contractile and relaxant responses of tracheal smooth muscle strips (TSM) and third-generation bronchi (3B) of control horses and horses with recurrent obstructive disease (heaves) were compared. Acetylcholine (ACH) sensitivity of the diseased tissues was less than that of tissues from control horses, especially at the level of the third generation (EC50 controls 15 +/- 11 microM vs 81 +/- 17 microM for heaveys). Despite tracheal and bronchial hyporesponsiveness to ACH, these tissues from heavey horses were hyperresponsive to EFS. The inhibitory effect of isoproterenol and electri...
Riley CB, Yovich JV.Fractures of the patella in the horse are infrequently reported
in the literature. Most of these have been associated with trauma
or sudden forceful contraction of the quadriceps resulting in an
avulsion fracture of the patella (Parks and Wyn-Jones 1988).
There have been few reports of fractures associated with des-
motomy of the medial patellar ligament to correct upward
fixation of the patella (Wright and Rose 1989; Gibson ef ul1989).
This paper describes one such case including the radiographic
findings before the desmotomy, after fracture of the patella and
after arthroscopic trea...
Weitkamp LR, Kenney RM, Bailey E, MacCluer JW, Brown JS, Blanchard TL, Sertich PL, Love CC, Hunt PR.Histological features of the endometrium, as assessed in biopsy samples, were related to Standardbred mare genotypes for transferrin, esterase (as a control) and equine leucocyte antigens (ELA). Pathological changes were found more frequently in each successively older age group of mares. Among mares aged 6-19 years, there were significant pathologic changes on first examination following an infertile breeding season for 46 of 90 (51%) of transferrin homozygotes and 50 of 146 (34%) of transferrin heterozygotes. The difference between the two groups was significant for the total data (chi 1(2) ...
Trotter GW, McIlwraith CW, Yovich JV, Norrdin RW, Wrigley RH, Lamar CH.The effects of the corticosteroid 6-alpha-methylprednisolone acetate on normal equine articular cartilage were evaluated, using the middle carpal joint in 4 clinically normal young horses. One middle carpal joint of each horse was injected 3 times with 100 mg of 6-alpha-methylprednisolone acetate, at 14-day intervals. The opposite middle carpal joint (control) was injected with 2.5 ml of lactated Ringer solution at the same intervals. Effects were studied until 8 weeks after the first injection. Evaluation included clinical and radiographic examination, and gross, microscopic, and biochemical ...
Trout DR, Hornof WJ, O'Brien TR.Radiography and soft tissue- and bone-phase scintigraphy were performed on 14 clinically normal horses and 35 horses in which definite, probable, or possible navicular disease had been diagnosed. The specificity of radiography and scintigraphy in revealing signs of navicular disease were nearly equal; however, the sensitivity of scintigraphy appeared to be greater than that of radiography. The greatest sensitivity and specificity were achieved when the results of radiography and scintigraphy were evaluated together. Differences in sensitivity were greatest when scintigraphy revealed lesions no...
Bjørn H, Sommer C, Schougård H, Henriksen SA, Nansen P.This study was undertaken to establish whether anthelmintic resistance was present in nematode parasites of horses in Denmark. Sixteen horse farms were selected for faecal egg count reduction (FECR) tests to measure the efficacy of the anthelmintic used. Resistance to benzimidazole anthelmintics was found on 13 of the 16 farms, with FECR values ranging from 80.0% to -101.3%. On the remaining 3 farms FECR was 100.0%, 99.3% and 97.2%. Results of a questionnaire study on anthelmintic usage, parasite control measures and management practices showed that horses in this study were treated on average...
Uhlinger C.A herd of 15 mature riding horses with a history of anorexia, weight loss, and lethargy was examined. The animals had been fed a 50/50 mixture of commercial sweet feed and corn screenings contaminated with a heavy growth of Fusarium moniliforme. Thirteen of the horses had one or more neurologic signs. The most characteristic signs were profound depression and mild ataxia. Over the 19-day course of the epizootic, the horses had increasing severe neurologic deficits, including unilateral blindness and delirium. Despite the clinical appearance of dehydration, 12 horses had low PCV (16 to 27%), he...
Schlindwein X, Werneburg I.We compared embryogenesis of five species of domesticated even-toed and one odd-toed ungulate and used a phylogenetic framework to contextualize such comparison. Organ systems that occur relatively earlier in embryogenesis generally have more time to develop and therefore are found to be more mature at birth when compared to structures that appear later in development. We hypothesized that the less mature the animals' organs are at birth, the more they are susceptible to artificial selection. The horse had the most mature organs at birth, followed by cattle, reindeer, sheep/goat, and pig. This...
Cohen JM, Schneider RK, Zubrod CJ, Sampson SN, Tucker RL.(1) To describe the clinical signs and abnormalities observed on magnetic resonance imaging (MRI) in 7 horses with desmitis of the distal digital annular ligament (DDAL); (2) to describe the normal magnetic resonance (MR) appearance and thickness of the DDAL in health; and (3) to describe a tenoscopic surgical technique for treating horses with desmitis of the DDAL. Methods: Retrospective study. Methods: Horses (n=7) with desmitis of the DDAL. Methods: MR examinations of 20 sound horses were reviewed to determine the normal appearance of the DDAL. Dimensions obtained from these images were com...
Asmis R, Jörg A.Horse eosinophils preincubated with 3H-labelled acetate and stimulated with the Ca2+ ionophores ionomycin or A23187 form a radioactive compound, which we have shown to be 1-O-alkyl-2-[3H]acetyl-sn-glycero-3-phosphocholine (platelet-activating factor). We could detect no 1-O-acyl-2-[3H]acetyl-glycero-3-phosphocholine in the radioactive fraction. The formation of platelet-activating factor was strongly correlated to the generation of leukotriene C4, the main arachidonate metabolite in horse eosinophils, suggesting that platelet-activating factor and leukotriene C4 have a common precursor pool (1...
Ludewig T.Corpora amylacea are present in all the mammary glands of the investigated animals (ewe, goat, and mares). Generally, they are comparable to the cow regarding to its distribution, frequency, size, morphological structure, and staining properties. Corpora amylacea occur most frequently in the ewe, and in non-lactating mammary glands. In lactating mammary glands they are mostly situated in the alveoles. During gestation they are located outside of the alveoles and in the interalveolar connective tissue. Only in very few cases corpora amylacea are found inside and outside of the alveoles in the s...
McElhone RL, Meakin GE, French JC, Alexander T, Morgan RM.A study was designed to investigate the effects of external variables, including blood type, flooring surface, footwear tread depth and blood dryness, on the appearance of blood-based footwear marks, with particular reference to simulating a specific casework scenario. Results showed that footwear marks left in human blood tended to be of greater quality than those in equine blood, highlighting a potential issue in applying data generated with equine blood to human bloodstains in casework. Footwear tread effects were also dependent on blood type, but the type of flooring surface did not affect...
Hoolahan DE, White SD, Outerbridge CA, Shearer PL, Affolter VK.Alopecia areata (AA) causes hair loss due to inflammatory changes within and around hair bulbs and lower portions of the hair follicles. Documentation of AA in horses is limited to a few case reports. Objective: The aim of this retrospective study was to characterize equine AA by describing patterns in age, sex, breed and lesion distribution in a series of cases. An attempt was made to characterize the long-term course of the disease by surveying owners of affected horses. Methods: Computerized records from 1 January 1980 to 1 July 2011 yielded 15 horses. Methods: Descriptive statistics were c...
Thurmon JC, Benson GJ.The purpose of this article is to review the use of selected anesthetics and anesthetic adjuncts in horses. Emphasis is placed on the pharmacologic bases of their use.
Inzana TJ, Iritani B.A group C latex agglutination reagent, included as the negative control in the PathoDx Strep A latex agglutination test (Diagnostic Products Corp., Los Angeles, Calif), was compared with culture for rapid detection of group C streptococci (Streptococcus equi, S. equisimilis, S. zooepidemicus, and S. dysgalactiae) from swabs of veterinary specimens. The overall sensitivity of the latex test was 78%, and specificity was 97.6%. Only 25% of S. dysgalactiae isolates were detected, thereby accounting for the relatively low sensitivity. Ninety-three percent of the group C streptococci other than S. d...
Kwok WH, Choi TLS, Tsoi YYK, Leung GNW, Wan TSM.A fast method for the direct analysis of enzyme-hydrolysed horse urine using an automated on-line solid-phase extraction (SPE) coupled to a liquid-chromatography/high resolution mass spectrometer was developed. Over 100 drugs of diverse drug classes could be simultaneously detected in horse urine at sub to low parts per billion levels. Urine sample was first hydrolysed by β-glucuronidase to release conjugated drugs, followed by centrifugal filtration. The filtrate (1mL) was directly injected into an on-line SPE system consisting of a pre-column filter and a SPE cartridge column for the separa...
Blancou J, Parsonson I.Since Roman Antiquity, domestic and wild animals have been transported over long distances for purposes as different as improvement of livestock production, food supply, scientific interest, public entertainment, war and numerous other purposes. This long distance transportation was originally limited to the Mediterranean area but, during the Middle Ages extended to the rest of Europe. The conquest of the New World was the first major occasion to transport large numbers of horses and other livestock across the oceans. Domestic animals were necessary for the new colonies and their armies. Europ...
Chung C, Wilson C, Timoney P, Adams E, Adams DS, Chung JS, Evermann JF, Shuck K, Lee SS, McGuire TC.Equine arteritis virus (EAV) causes contagious equine viral arteritis, characterized by fever, anorexia, conjunctivitis, nasal discharge, dependent edema, abortion, infrequent death in foals, and establishment of the carrier state in stallions. The World Organization for Animal Health (OIE) defines a horse as seropositive if the serum neutralization (SN) antibody titer is ≥1:4 to EAV. However, determining the SN titer is time-consuming and requires specific laboratory facilities, equipment, and technical expertise to perform. Furthermore, interpretation of the SN titer of some sera can be di...
True RG, Lowe JE.Juglone, a toxic compound found in all parts of plants of the walnut tree family Jugans, was evaluated as the possible toxin involved in black walnut shaving-associated laminitis in the horse. Large amounts (up to 1 g) of this chemical administered per os inconsistently caused mild signs of laminitis in ponies. Topical application of juglone to the digits of horses caused local skin irritation but did not cause laminitis. Intravenous administration of juglone caused acute pulmonary edema in some individuals previously exposed to the compound per os or IV.
Smith RK, Dyson SJ, Head MJ, Butson RJ.The ultrasonographic appearance of the equine triceps muscle of clinically normal horses, before and after general anaesthesia, was investigated and compared with 5 cases of post anaesthetic myopathy. The triceps muscle areas were examined bilaterally using a 7.5 MHz linear array probe in 2 different planes, with each limb both weightbearing and nonweightbearing. The triceps muscles of 4 unanaesthetised horses were scanned twice, 24 h apart. Six horses underwent general anaesthesia and were scanned pre-anaesthesia and at 1 and 24 h intervals after recovery. Blood samples were obtained in the u...
Waugh SL, Fregin GF, Thomas DP, Gerber N, Grant BD, Campbell KB.Aortic root blood flow was measured with an electromagnetic (EM) flow meter in unanesthetized horses during rest and exercise. The cardiac output response to exercise, as determined by the EM technique, was compared with the response reported by others who used indicator dilution techniques with good agreement. The EM method will allow making measurement of cardiovascular responses to exercise in the horse, not obtainable with other techniques.
Chun J, Kang T, Seo JP, Jeong H, Kim M, Kim BS, Ahn M, Kim J, Shin T.Vomeronasal organ (VNO) is a tubular pheromone sensing organ in which the lumen is covered with sensory and non-sensory epithelia. This study used immunohistochemistry and lectin histochemistry techniques to evaluate developmental changes, specifically of the glycoconjugate profile, in the horse VNO epithelium. Immunostaining analysis revealed PGP9.5 expression in some vomeronasal non-sensory epithelium (VNSE) cells and in the vomeronasal receptor cells of the vomeronasal sensory epithelium (VSE) in fetuses, young foals, and adult horses. OMP expression was exclusively localized in receptor ce...
Perryman LE, Magnuson NS.Significant contributions to understanding the role of lymphocyte subpopulations in the immune response and to the characterization of immunodeficiencies in children have been achieved through study of animal models of immunodeficiency. Additional contributions can be made in two important areas. One is through identification of relevant, naturally-occurring models of adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency. The second, and potentially more important contribution, would be the identification of the metabolic basis for existing immune deficiencies. The nece...
Ahn B, Zhang Y, Osterrieder N, O'Callaghan DJ.The 150 kbp genome of equine herpesvirus-1 (EHV-1) is composed of a unique long (UL) region and a unique short (Us) segment, which is flanked by identical internal and terminal repeat (IR and TR) sequences of 12.7 kbp. We constructed an EHV-1 lacking the entire IR (vL11ΔIR) and showed that the IR is dispensable for EHV-1 replication but that the vL11ΔIR exhibits a smaller plaque size and delayed growth kinetics. Western blot analyses of cells infected with vL11ΔIR showed that the synthesis of viral proteins encoded by the immediate-early, early, and late genes was reduced at immediate-early...
Bowker RM, Sonea IM, Vex KB, Caron JP.Substance P (SP) immunocytochemistry and receptor autoradiography were used to define the innervation of the equine synovial membrane of joints equivalent to the wrist and knuckle of man. SP-immunoreactive fibers were mainly concentrated around blood vessels in the subsynovial layer, although not exclusively, while in the more distal joint, SP fibers were more frequently seen in the synovial surface layer. Iodinated SP receptor autoradiography studies revealed silver grain concentrations in the advential layer of blood vessels associated with the vasa vasorum, on the vascular endothelium and i...
Flores M, Wajnberg E, Bemski G.Electron nuclear double resonance (ENDOR) spectroscopy has been used to study protons in nitrosyl horse heart myoglobin (MbNO). (1)H ENDOR spectra were recorded for different settings of the magnetic field. Detailed analysis of the ENDOR powder spectra, using computer simulation, based on the "orientation-selection" principle, leads to the identification of the available protons in the heme pocket. We observe hyperfine interactions of the N(HisF8)-Fe(2+)-N(NO) complex with five protons in axial and with eight protons in the rhombic symmetry along different orientations, including those of the ...
Taylor D, Hood DM, Wagner IP.To evaluate the short-term effects of 4 therapeutic shoeing systems on lameness and voluntary limb-load distribution in horses with chronic laminitis. Methods: 10 horses with chronic laminitis. Methods: A clinical trial was conducted that used a concurrent control, crossover design to evaluate the relative effectiveness of a standard flat shoe, fullered egg-bar shoe, heart-bar shoe, and modified equine digital support system to alleviate chronic lameness in horses. Therapeutic success was assessed during a 7-day period by use of subjective (Obel grade and clinical score) and objective (force-p...