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Topic:Tissue

Tissue in horses refers to the various types of biological material that make up the body of the animal, including muscle, connective, epithelial, and nervous tissues. Each type of tissue has specific functions and characteristics, contributing to the overall physiology and health of the horse. Muscle tissue is responsible for movement and locomotion, connective tissue provides structural support and protection, epithelial tissue covers body surfaces and lines cavities, and nervous tissue is involved in transmitting signals for communication and coordination within the body. The study of equine tissue encompasses aspects such as growth, repair, and response to injury or disease. This page compiles peer-reviewed research studies and scholarly articles that explore the structure, function, and pathology of different tissue types in horses, offering insights into their role in equine health and disease management.
Synovial fluid levels and serum pharmacokinetics in a large animal model following treatment with oral glucosamine at clinically relevant doses.
Arthritis and rheumatism    January 11, 2005   Volume 52, Issue 1 181-191 doi: 10.1002/art.20762
Laverty S, Sandy JD, Celeste C, Vachon P, Marier JF, Plaas AH.To examine the concentration of glucosamine in the synovial fluid and its pharmacokinetics in serum in a large animal model following dosing with glucosamine HCl at clinically relevant levels. Methods: Eight adult female horses were studied. After an overnight fast, glucosamine HCl (20 mg/kg of body weight) was administered by either nasogastric (NG) intubation or intravenous (IV) injection. Blood samples were collected before dosing and at 5, 15, 30, 60, 120, 180, 240, 360, 480, and 720 minutes after dosing. Synovial fluid samples were collected from the radiocarpal joints 48 hours before dos...
Distinct fibro-vascular arrangements in the periodontal ligament of the horse.
Archives of oral biology    December 8, 2004   Volume 50, Issue 4 439-447 doi: 10.1016/j.archoralbio.2004.10.001
Staszyk C, Gasse H.The periodontal ligament (PDL) of the hypsodont cheek teeth of the horse meets specific requirements of tooth support: (1) to fix the tooth; (2) to withstand pressure and tension during the laterolateral mastication movement; (3) to restore the original tooth position. These requirements are assumed to be met by the periodontal collagen fiber apparatus and by the vascular system. Distinct fibro-vascular arrangements are described here in order to reveal the structural prerequisites for these distinct functions. Methods: Specimens from nine horses were processed for (1) collagen labeling with f...
Surgical management of compound odontoma in two horses.
Journal of the American Veterinary Medical Association    November 24, 2004   Volume 225, Issue 9 1423-1393 doi: 10.2460/javma.2004.225.1423
Brounts SH, Hawkins JF, Lescun TB, Fessler JF, Stiles P, Blevins WE.Two horses were admitted for evaluation of mandibular swelling (horse 1) or maxillary distortion (horse 2). Both horses had radiographic evidence of tumors of dental origin that had the appearance of a compound odontoma. Extensive surgical resection was performed for treatment. Horse 1 was treated with 1-stage surgical resection, but an iatrogenic fracture occurred during surgery, which was managed successfully with a type I external fixator and extraoral alimentation. Horse 2 was treated in multiple stages to remove all portions of the tumor. To manage an extensive orosinal fistula, a custom-...
Rapid analysis of fatty acid-binding proteins with immunosensors and immunotests for early monitoring of tissue injury.
Biosensors & bioelectronics    November 18, 2004   Volume 20, Issue 12 2566-2580 doi: 10.1016/j.bios.2004.09.028
Chan CP, Wan TS, Watkins KL, Pelsers MM, Van der Voort D, Tang FP, Lam KH, Mill J, Yuan Y, Lehmann M, Hempel A, Sanderson JE, Glatz JF, Renneberg R.Fatty acid-binding protein (FABP) holds promise for early detection of tissue injury. This small protein (15kD) appears earlier in the blood than large proteins after cell damage. Combined its characteristics of high concentration tissue contents and low normal plasma values provide the possibility of a rapid rise above the respective reference values, and thus an early indication of the appearance of tissue injury. A general review was presented on the current status of different types of FABP for the detection of tissue injury in patients with myocardial injury, brain injury and also in athl...
Engineered articular cartilage: influence of the scaffold on cell phenotype and proliferation.
Journal of materials science. Materials in medicine    September 7, 2004   Volume 14, Issue 8 713-716 doi: 10.1023/a:1024915817061
Gigante A, Bevilacqua C, Cappella M, Manzotti S, Greco F.Articular cartilage defects do not heal. Biodegradable scaffolds have been studied for cartilage engineering in order to implant autologous chondrocytes and help cartilage repair. We tested some new collagen matrices differing in collagen type, origin, structure and methods of extraction and purification, and compared the behavior of human chondrocytes cultured on them. Human chondrocytes were grown for three weeks on four different equine type I collagen matrices, one type I, III porcine collagen matrix and one porcine type II collagen matrix. After 21 days, samples were subjected to histoche...
The development and distribution of the interstitial cells of Cajal in the intestine of the equine fetus and neonate.
Journal of anatomy    July 17, 2004   Volume 205, Issue 1 35-44 doi: 10.1111/j.0021-8782.2004.00315.x
Fintl C, Pearson GT, Ricketts SW, Mayhew IG, Hudson NP.This study set out to determine the pattern of development and distribution of the interstitial cells of Cajal (ICC) in the intestinal tract of the equine fetus and neonate. Intestinal tissue samples from 12 naturally aborted equine fetuses and three euthanized neonates were collected and fixed in formalin prior to applying standard immunohistochemical labelling techniques targeting the c-Kit protein of the ICC. At 6 months of gestation, a network of ICC was present in the myenteric plexus region of both the small and the large intestine. ICC were also present within the circular muscle layer....
Exercise-induced oxidative stress and muscle stress protein responses in trotters.
European journal of applied physiology    June 23, 2004   Volume 93, Issue 4 496-501 doi: 10.1007/s00421-004-1162-x
Kinnunen S, Hyyppä S, Lappalainen J, Oksala N, Venojärvi M, Nakao C, Hänninen O, Sen CK, Atalay M.Acute exercise induces oxidative stress and heat shock protein (HSP) expression. Information on the protection of stress proteins against oxidant insult and muscle damage during moderate exercise is scanty. We aimed to show how a single bout of moderate exercise affects the markers of oxidative stress and heat shock factor-1 (HSF1; the transcriptional regulator of HSP synthesis), and HSP70, HSP90 and glucose-regulated protein (GRP75) expression in horses. Eight clinically normal and regularly trained standardbred trotters were treadmill-exercised for 45 min at moderate intensity. Blood samples...
Detection of West Nile virus using formalin fixed paraffin embedded tissues in crows and horses: quantification of viral transcripts by real-time RT-PCR.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology    May 28, 2004   Volume 30, Issue 4 320-325 doi: 10.1016/j.jcv.2004.01.003
Tewari D, Kim H, Feria W, Russo B, Acland H.West Nile virus (WNV) RNA was quantified in WNV infected crows and horses with the help of a real-time reverse transcriptase-PCR assay. A 5' nuclease assay, based on NS5 gene detection with a fluorescent probe was used for quantifying WNV RNA using formalin fixed paraffin embedded tissue specimens. Quantitative detection of WNV RNA showed the presence of a higher amount of the viral RNA in crow tissues compared to equine tissues and these results correlated well with the detection of WNV antigen by immunostaining. In crows, the highest amount of virus was seen in the intestine and in horses in...
Hoof wall wound repair.
Equine veterinary journal    May 19, 2004   Volume 36, Issue 3 210-215 doi: 10.2746/0425164044877189
Pollitt CC, Daradka M.Surgical stripping of the hoof wall results in a wound that heals remarkabley well. In contrast, lamellae recovering from laminitis are often deformed. Investigating lamellar wound healing may aid understanding of laminitis. Objective: To document temporal changes in the lamellar basement membrane (BM), dermis and epidermis after surgery. Methods: Wall strips were made in the dorsal hoof wall midline of 6 mature horses. Immunohistochemistry was used to document changes in the basement membrane (BM) and detect proliferation of epidermal cells in lamellar tissues harvested at intervals. A confor...
Equine laminitis: glucose deprivation and MMP activation induce dermo-epidermal separation in vitro.
Equine veterinary journal    May 19, 2004   Volume 36, Issue 3 261-266 doi: 10.2746/0425164044877170
French KR, Pollitt CC.Acute laminitis is characterised by hoof lamellar dermal-epidermal separation at the basement membrane (BM) zone. Hoof lamellar explants cultured in vitro can also be made to separate at the basement membrane zone and investigating how this occurs may give insight into the poorly understood pathophysiology of laminitis. Objective: To investigate why glucose deprivation and metalloproteinase (MMP) activation in cultured lamellar explants leads to dermo-epidermal separation. Methods: Explants, cultured without glucose or with the MMP activator p-amino-phenol-mercuric acetate (APMA), were subject...
Sirius red is able to selectively stain eosinophil granulocytes in bovine, ovine and equine cervical tissue.
Anatomia, histologia, embryologia    May 18, 2004   Volume 33, Issue 3 180-182 doi: 10.1111/j.1439-0264.2004.00532.x
Wehrend A, Hetzel U, Huchzermeyer S, Klein C, Bostedt H.The aim of the present study was to examine the suitability of sirius red staining for selective light microscopic demonstration of eosinophil granulocytes in cervical tissue of mares, cows and sheep. For this purpose, tissue was fixed in 4% neutral buffered formol or in Bouin's solution. Paraffin sections of 5-microm thickness were stained with sirius red. In cows, mares and sheep a selective distinction of eosinophilic infiltration is successful after both fixation methods.
Desmoplakin and Plakoglobin–specific markers of lymphatic vessels in the skin?
Anatomia, histologia, embryologia    May 18, 2004   Volume 33, Issue 3 168-171 doi: 10.1111/j.1439-0264.2004.00529.x
Fedele C, Berens D, Rautenfeld V, Pabst R.Monoclonal antibodies against Desmoplakin and Plakoglobin were tested for their suitability as specific markers of lymphatic vessels. The tissue samples were taken from horse skin in an attempt to establish the horse as a model for human lymphatic diseases. To obtain a clear, positive identification of blood and lymphatic vessels, immunohistochemical staining with antibodies against vascular endothelial growth factor receptor 3 (VEGFR-3) and platelet endothelial adhesion molecule (PECAM-1, CD31), was compared with Desmoplakin and Plakoglobin. Because anti-VEGFR-3 is specific for lymphatic vess...
Assessment of apoptosis in epidermal lamellar cells in clinically normal horses and those with laminitis.
American journal of veterinary research    May 15, 2004   Volume 65, Issue 5 578-585 doi: 10.2460/ajvr.2004.65.578
Faleiros RR, Stokes AM, Eades SC, Kim DY, Paulsen DB, Moore RM.To determine and compare the number, type, location, and distribution of apoptotic epidermal cells in the laminae of clinically normal horses and horses with laminitis. Methods: Formalin-fixed samples of digital lamellar tissue from 47 horses (including clinically normal horses [controls; n = 7], horses with acute [4] and chronic [7] naturally acquired laminitis, and horses with black walnut extract-induced [11] or carbohydrate overload-induced [18] laminitis). Methods: Blocks of paraffin-embedded lamellar tissues were stained for DNA fragmentation with the terminal deoxynucleotidyl transferas...
Benign fibrous histiocytoma of prepuce in the mule.
Reproduction in domestic animals = Zuchthygiene    May 8, 2004   Volume 39, Issue 1 61-63 doi: 10.1046/j.1439-0531.2003.00475.x
Rapisarda G, Marino F, Ferrara G, Gioffrè F, Galofaro V.Here we present a case of preputial benign fibrous histiocytoma in a 7-year-old mule. This case was treated surgically. Pathologic analysis and diagnosis of these lesions were based on a combination of light microscopy and immunohistochemistry. Histologically, the tumour was composed primarily of a storiform pattern of fibroblastic and poligonal cells. In immunohistochemistry, except for consistent immunoreactivity for vimentin, nearly all tumour cells were positive for CD68, S-100 protein, pan-cytokeratin, alpha-smooth muscle actin, and desmin. Benign fibrous histiocytoma of the prepuce in mu...
A simple fluorescence labeling method to visualize the three-dimensional arrangement of collagen fibers in the equine periodontal ligament.
Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische Gesellschaft    May 6, 2004   Volume 186, Issue 2 149-152 doi: 10.1016/S0940-9602(04)80030-X
Staszyk C, Gasse H.In order to display the collagen-fiber arrangement in the equine periodontal ligament an inexpensive and easy staining procedure with fluorescein was applied to paraffin sections. After fluorescein labeling a section was suitable for successful examination with three special microscopical systems: a) fluorescence microscopy b) phase contrast microscopy and c) polarized light microscopy. Collagen fibers were clearly displayed as compact structures in the fluorescence microscope. This distinct feature of the fluorescent image generated an almost three-dimensional impression of the fiber arrangem...
Equine beta-defensin-1: full-length cDNA sequence and tissue expression.
Veterinary immunology and immunopathology    April 29, 2004   Volume 99, Issue 1-2 127-132 doi: 10.1016/j.vetimm.2003.12.010
Davis EG, Sang Y, Blecha F.beta-Defensins are cysteine-rich endogenously produced antimicrobial peptides that play an important role in innate immune defense. Although, previous investigations have identified beta-defensins in several mammalian species, no reports have identified equine beta-defensins. Using a strategy of database searching for expressed sequence tags (EST) we identified putative expression of equine beta-defensins in hepatic tissue. Based on this information, sequence specific primers were designed for the equine gene enabling the identification of the full-length cDNA sequence of equine beta-defensin-...
Tissue distribution of clenbuterol in the horse.
Journal of veterinary pharmacology and therapeutics    April 21, 2004   Volume 27, Issue 2 91-98 doi: 10.1111/j.1365-2885.2004.00558.x
Soma LR, Uboh CE, Guan F, Luo Y, Teleis D, Runbo L, Birks EK, Tsang DS, Habecker P.Plasma and tissue concentrations of clenbuterol (CLB) were determined following oral (p.o.) administration of 1.6 microg/kg twice daily (b.i.d.) for 2 weeks. Horses were administered the last dose on morning of day 15, killed at 0.25, 24, 48, and 72 h post-administration. At 0.25 h, the highest tissue concentrations of CLB were found in the liver (16.21 ng/g), lung (6.48 ng/g), left ventricle (4.99 ng/g), kidney (3.35 ng/g), bronchi (2.56 ng/g), right ventricle (2.08 ng/g), and eye fluids (1.09 ng/g) all of which were higher than that of plasma (1.10 ng/mL). The elimination half-lives (t(1/2k)...
Immunohistochemical and functional evidence for a noradrenergic regulation in the horse penile deep dorsal vein.
International journal of impotence research    April 2, 2004   Volume 16, Issue 6 486-491 doi: 10.1038/sj.ijir.3901228
Recio P, Prieto D, Martínez MP, García P, Rivera L, Benedito S, Martínez AC, Sacristán AG, Orensanz LM, Hernández M.Our aim was to study the presence of noradrenergic nerves and to characterize the alpha-adrenergic receptors involved in the contractions to electrical field stimulation and to alpha-adrenergic agonists of the horse penile deep dorsal vein. Noradrenergic fibres were visualized by immunohistochemistry using an antibody against dopamine-beta-hydroxylase (DBH). For functional studies, the responses of the venous rings to electrical field stimulation and to alpha-adrenergic agonists (noradrenaline, phenylephrine and BHT 920) were studied in the absence and the presence of noradrenergic transmissio...
Oxytalan fibres in the periodontal ligament of equine molar cheek teeth.
Anatomia, histologia, embryologia    March 19, 2004   Volume 33, Issue 1 17-22 doi: 10.1111/j.1439-0264.2004.00503.x
Staszyk C, Gasse H.The distribution and arrangement of oxytalan fibres were examined in periodontal specimens of cheek teeth from seven horses. Oxidation prior to aldehyde fuchsin exposition permitted a selective staining of the oxytalan fibres, which are a distinct component of the elastic fibre system. On three horizontal levels of the periodontium--(a) subgingival, (b) middle third and (c) apical--two oxytalan fibre groups were shown histologically: 'blood vessel-related' and 'independent' oxytalan fibres. In levels a and b, both groups were arranged in a typical occluso-apical alignment along the reserve cro...
Matrix metalloproteinase-2 and -9 secretion by the equine ovary during follicular growth and prior to ovulation.
Animal reproduction science    March 5, 2004   Volume 81, Issue 3-4 329-339 doi: 10.1016/j.anireprosci.2003.10.011
Riley SC, Thomassen R, Bae SE, Leask R, Pedersen HG, Watson ED.Profound hormonally controlled tissue remodelling occurs in the equine ovary for follicle growth and development, and also for the alteration in follicle shape directed towards the ovulation fossa, the site where ovulation occurs. The aim of this study was to examine the spatial and temporal regulation of matrix metalloproteinases (MMP)-2 and MMP-9, important enzymes in tissue remodelling, during follicle growth, and ovulation. Using gelatin substrate zymography, we measured these MMPs in follicular fluid of large anovulatory follicles collected during spring transition, early dominant follicl...
Repair of extensive articular cartilage defects in horses by autologous chondrocyte transplantation.
Annals of biomedical engineering    February 18, 2004   Volume 32, Issue 1 57-69 doi: 10.1023/b:abme.0000007791.81433.1a
Litzke LE, Wagner E, Baumgaertner W, Hetzel U, Josimović-Alasević O, Libera J.Damaged adult articular cartilage has very limited capacity to heal. Autologous chondrocyte transplantation (ACT) has been used clinically and studied in experimental animals in an attempt to provide biologically based cartilage regeneration. This study evaluated cartilage repair following ACT in a large animal model over a period of 2 years. Articular cartilage defects (10 mm in diameter, full-thickness) were created in the minor load-bearing area on the lateral talus of tibiotarsal joints of eight adult horses. In each animal, the right joint was repaired using autologous chondrocytes inject...
Influence of different collagen species on physico-chemical properties of crosslinked collagen matrices.
Biomaterials    February 14, 2004   Volume 25, Issue 14 2831-2841 doi: 10.1016/j.biomaterials.2003.09.066
Angele P, Abke J, Kujat R, Faltermeier H, Schumann D, Nerlich M, Kinner B, Englert C, Ruszczak Z, Mehrl R, Mueller R.Collagen-based scaffolds are appealing products for the repair of cartilage defects using tissue engineering strategies. The present study investigated the species-related differences of collagen scaffolds with and without 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC)/N-hydroxysuccinimide (NHS)-crosslinking. Resistance against collagenase digestion, swelling ratio, amino acid sequence, shrinkage temperature, ultrastructural matrix morphology, crosslinking density and stress-strain characteristics were determined to evaluate the physico-chemical properties of equine- and bovine-collagen...
Adhesion and proliferation of human dermal fibroblasts on collagen matrix.
Journal of biomaterials applications    February 12, 2004   Volume 18, Issue 3 209-222 doi: 10.1177/0885328204039692
Croce MA, Silvestri C, Guerra D, Carnevali E, Boraldi F, Tiozzo R, Parma B.The purpose of this study was to evaluate adhesion and growth of human dermal fibroblasts on a 0.150 mm-thick matrix of reconstituted collagen isolated from horse tendon. Collagen was extracted and polymerized according to the standard procedures (Opocrin, Corlo, Modena, Italy). By light microscopy, the bottom surface of the matrix appeared linear and compact, whereas the superficial one was indented and less homogeneous. By scanning electron microscopy, the collagen fibrils had different diameters and the great majority of them was oriented parallel to the surface of the gel. By transmission ...
Tissue-specific dysregulation of cortisol metabolism in equine laminitis.
Equine veterinary journal    February 6, 2004   Volume 36, Issue 1 41-45 doi: 10.2746/0425164044864750
Johnson PJ, Ganjam VK, Slight SH, Kreeger JM, Messer NT.The role of glucocorticoids (GCs) in the pathogenesis of laminitis is incompletely understood. Local tissue activity of GC is regulated by the steroid converting enzyme, 11beta-hydroxysteroid dehydrogenase-1 (11beta-HSD-1). Changes in integumentary (skin and hoof lamellar) 11beta-HSD activity occurring during laminitis could affect the extent to which GCs are involved in its development. Objective: That changes in integumentary 11beta-HSD-1 activity associated with the laminitic condition would lead to elevated local tissue levels of GCs, which could subsequently contribute, through paracrine ...
Spatial and temporal expression of types I and II receptors for transforming growth factor beta in normal equine skin and dermal wounds.
Veterinary surgery : VS    December 23, 2003   Volume 33, Issue 1 70-76 doi: 10.1111/j.1532-950x.2004.04008.x
De Martin I, Theoret CL.To describe immunolocalization of TGF-beta receptors (RI and RII) in normal equine skin and in thoracic or limb wounds, healing normally or with exuberant granulation tissue (EGT). Methods: Group A: six wounds on one metacarpus and one midthoracic area. Group B: six wounds on both metacarpi, one of which was bandaged to stimulate EGT. Immunohistochemistry was used to detect RI and RII expression in wound margins. Methods: Eight horses, randomly assigned to one of two study groups. Methods: Neutralizing polyclonal anti-rabbit RI and RII antibodies were used to detect spatial expression of RI an...
The equine thymus microenvironment: a morphological and immunohistochemical analysis.
Developmental and comparative immunology    December 4, 2003   Volume 28, Issue 3 251-264 doi: 10.1016/s0145-305x(03)00134-4
Contreiras EC, Lenzi HL, Meirelles MN, Caputo LF, Calado TJ, Villa-Verde DM, Savino W.We characterized herein the microarchitecture of the equine thymus along with post-natal development (6 months-->18 years). Thymuses showed an involutional process, beginning before the puberty and defined by five histological grades, which consider the progressive cortical thymocyte depletion, shrinkage and rearrangement of the epithelial network and increase in extracellular matrix (ECM). A second feature of the equine thymus was the presence of eosinopoiesis, erythropoiesis, mastocytopoiesis and plasmacytogenesis. Additionally, lymphatic vessels, full of lymphocytes, were particularly pr...
Effects of acetylcysteine and migration of resident eosinophils in an in vitro model of mucosal injury and restitution in equine right dorsal colon.
American journal of veterinary research    November 5, 2003   Volume 64, Issue 10 1205-1212 doi: 10.2460/ajvr.2003.64.1205
Rötting AK, Freeman DE, Eurell JA, Constable PD, Wallig M.To evaluate the in vitro protective effects of acetylcysteine and response of resident mucosal eosinophils in oxidant-induced injury to tissues of right dorsal colon of horses. Methods: 9 adult horses. Methods: Gastrointestinal mucosa was damaged in vitro with 3 mM hypochlorous acid (HOCl), with and without prior exposure to 6mM acetylcysteine. Control tissues were not exposed to HOCl or acetylcysteine. Control and damaged tissues were incubated in Krebs-Ringer-bicarbonate solution and tissue resistance measured during 240 minutes. Tissue permeability to radiolabeled mannitol was also used to ...
Structural changes in loaded equine tendons can be monitored by a novel spectroscopic technique.
The Journal of physiology    October 24, 2003   Volume 554, Issue Pt 3 791-801 doi: 10.1113/jphysiol.2003.054809
Kostyuk O, Birch HL, Mudera V, Brown RA.This study aimed to investigate the preferential collagen fibril alignment in unloaded and loaded tendons using elastic scattering spectroscopy. The device consisted of an optical probe, a pulsed light source (320-860 nm), a spectrometer and a PC. Two probes with either 2.75 mm or 300 microm source-detector separations were used to monitor deep and superficial layers, respectively. Equine superficial digital flexor tendons were subjected to ex vivo progressive tensional loading. Seven times more backscattered light was detected parallel rather than perpendicular to the tendon axis with the 2.7...
Immunohistochemical study of equine endometrial extracellular matrix during the oestrous cycle.
Journal of comparative pathology    October 14, 2003   Volume 129, Issue 4 316-319 doi: 10.1016/s0021-9975(03)00048-3
Mansour GD, Henry M, Ferreira AM.This study was designed to identify the distribution of type IV collagen, laminin, and fibronectin with an avidin-biotin method in sections of equine endometrial samples, fixed in Bouin's solution and embedded in paraffin wax. Thirty endometrial biopsies were collected at three different stages of the oestrous cycle. The basement membrane of luminal epithelium reacted positively with antibody against type IV collagen. Both type IV collagen and laminin were found in the basement membranes of endometrial glands, and fibronectin occurred diffusely in the interstitial tissue. Blood vessels express...
[Thermal injury and wound healing of the endometrium subsequent to minimally invasive transendoscopic use of Nd:YAG-laser-and electrosurgery in horses].
DTW. Deutsche tierarztliche Wochenschrift    August 13, 2003   Volume 110, Issue 7 271-280 
Bartmann CP, Stief B, Schoon HA.Transendoscopic thermal preparation techniques like laser- or electrosurgery have proved to be most reliable for minimal invasive intrauterine surgery during operative hysteroscopy in mares. To determine the effect of Nd:YAG laser surgery on the endometrium and the complete uterine wall compared with electrosurgery, standard lesions were obtained by transendoscopic monopolar electrosurgery (loop electrode, cutting blade) and Nd:YAG laser surgery (contact, bare fibre, 25 Watt, exposure time four seconds, non contact, 80 Watt, exposure time four seconds) in five healthy mares on days d 0, 7, 14,...
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