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Topic:Veterinary Research
Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
Resistance of Rhodococcus equi to acid pH. Rhodococcus equi is an important gram-positive intracellular facultative pathogen in foals of less than 3 months of age, that causes suppurative bronchopneumonia, lymphadenitis and/or enteritis. The disease in young foals mainly occurs in spring and summer when weather conditions are favorable for survival and multiplication of the bacteria in the environment. R. equi is widespread in the environment of horsebreeding farms: it has been isolated from the soil of paddocks and from the feces of adult horses and foals. Aerosol infection via dust of paddocks seems to be the major route of foal infe...
Factors associated with time until first race and career duration for Thoroughbred racehorses. To determine whether sex or foaling period were associated with time to first race start and whether sex, age at time of first race start, or inter-race period were associated with career duration for Thoroughbred racehorses in Australia. Methods: 553 Thoroughbred racehorses. Methods: Race records through to the end of the 6-year-old racing season were obtained for each horse. Product-limit survival analysis was used to determine whether sex or foaling period was associated with time to first race start and whether sex or age at first race was associated with career duration. A Cox proportiona...
Development of a fluorescence polarization-based diagnostic assay for equine infectious anemia virus. The control of equine infectious anemia virus (EIAV) infections of horses has been over the past 20 years based primarily on the identification and elimination of seropositive horses, predominantly by a standardized agar gel immunodiffusion (AGID) assay in centralized reference laboratories. This screening for EIAV-seropositive horses has been to date hindered by the lack of a rapid diagnostic format that can be easily employed in the field. We describe here the development of a rapid solution-phase assay for the presence of serum antibodies to EIAV based on fluorescence polarization (FP) (pat...
Osteoclastic resorption of equine cranial and postcranial bone in vitro. To address possible differences in the resorbability of cranial and postcranial bone, slices of equine frontal bone and leg (first phalanx or third metacarpus) were seeded with embryonic chick bone cells and cultured for 20-24h. After removing the cells and drying the specimens, the areas and volumes of more than 800 resorption pits in each set were measured using a video-rate reflection confocal microscope system. Relative mineralization densities were determined by quantitative electron backscattering analysis. The mean mineralization density was greater in the leg bone, but the mean depths ...
Use of a non-linear spline regression to model time-varying fluctuations in mammary-secretion element concentrations of periparturient mares in Michigan, USA. A study was designed to model the fluctuations of nine specific element concentrations in mammary secretions from periparturient mares over time. During the 1992 foaling season, serial samples of mammary secretions were collected from all 18 pregnant Arabian mares at the Michigan State University equine teaching and research center. Non-linear regression techniques were used to model the relationship between element concentration in mammary secretions and days from foaling (which connected two separate sigmoid curves with a spline function); indicator variables were included for mare and mare ...
Scintigraphic characterization of distal radial physeal closure in young Standardbred racehorses. A longitudinal scintigraphic and radiographic study of distal radial physeal activity in 14 Standardbred trotters was conducted to quantify and visually evaluate physeal uptake around the time of physeal closure. Initial intense 99mTc-HDP uptake was seen, followed by a rapid decrease of activity starting at a mean age of 20 months. Mean physeal to distal diaphyseal ROI and line profile ratios compared to ratios of mature horses decreased from 2.8 to 1.4 at the time of radiographic closure at 24-32 months. Ratios then gradually decreased until values of mature horses were reached at the end of ...
Low-field magnetic resonance imaging of the equine tarsus: normal anatomy. The objective of this study was to define the normal gross anatomic appearance of the adult equine tarsus on a low-field magnetic resonance (MR) image. Six radiographically normal, adult, equine tarsal cadavers were utilized. Using a scanner with a 0.064 Tesla magnet, images were acquired in the sagittal, transverse and dorsal planes for T1-weighted and the sagittal plane for T2-weighted imaging sequences. Anatomic structures on the MR images were identified and compared with cryosections of the imaged limbs. Optimal image planes were identified for the evaluation of articular cartilage, subch...
Cardiorespiratory impact of the nitric oxide synthase inhibitor L-NAME in the exercising horse. To investigate the role of nitric oxide, NO, in facilitating cardiorespiratory function during exercise, five horses ran on a treadmill at speeds that yielded 50, 80 and 100% of peak pulmonary oxygen uptake (V(O(2)) peak) as determined on a maximal incremental test. Each horse underwent one control (C) and one (NO-synthase inhibitor; N(G)-L-nitro-arginine methyl ester (L-NAME), 20 mg/kg) trial in randomized order. Pulmonary gas exchange (open flow system), arterial and mixed-venous blood gases, cardiac output (Fick Principle), and pulmonary and systemic conductances were determined. L-NAME red...
Binding of equine infectious anemia virus rev to an exon splicing enhancer mediates alternative splicing and nuclear export of viral mRNAs. In addition to facilitating the nuclear export of incompletely spliced viral mRNAs, equine infectious anemia virus (EIAV) Rev regulates alternative splicing of the third exon of the tat/rev mRNA. In the presence of Rev, this exon of the bicistronic RNA is skipped in a fraction of the spliced mRNAs. In this report, the cis-acting requirements for exon 3 usage were correlated with sequences necessary for Rev binding and transport of incompletely spliced RNA. The presence of a purine-rich exon splicing enhancer (ESE) was required for exon 3 recognition, and the addition of Rev inhibited exon 3 sp...
[Veterinary service and supervision of equine competitions]. Because of the growing interest in animal health and welfare in breeding and sport--specially in the horse--the regulation of the German Riding Association (FN) was updated. The result--the LPO 2000--refer more functions to the veterinarian. At first the permanent presence of the vet is necessary, at second he controls the correct, new installed vaccination against influenza-virus. The functions of vet-check, general horse-control and doping are wide up in number and consultation. For this the veterinarian must decree about much experience and good preparation. The organisations of the veterin...
Measuring sphere-like structures using transrectal ultrasonography. Sphere-like structures are commonly measured for clinical or research purposes. We measured two diameters, the circumference (perimeter) and cross sectional area of 113 equine ovarian follicles in a water bath then determined the relationships between those measurements and the actual volume of fluid in the follicles. Cross-sectional area, diameter3 and area2/3 were all highly correlated with the volume of the structures and superior (p 0.05) between cross-sectional area, diameter3, and area2/3. Because of the ease of measuring diameter, we suggest that diameter3 be used to estimate the actua...
Simultaneous determination of hydrocortisone, dexamethasone, indomethacin, phenylbutazone and oxyphenbutazone in equine serum by high-performance liquid chromatography. Ethyl acetate extracts of equine serum, containing 0-5 microg/ml of hydrocortisone (HYD), dexamethasone (DEX), oxyphenbutazone (OPB), indomethacin (IND), phenylbutazone (PB) and probenecid as internal standard, were evaporated with nitrogen, resuspended in methanol and analyzed by HPLC, using a C-18 column equilibrated with 51:49 acetonitrile-water, 0.1% trifluoroacetic acid, at 1 ml/min. The eluate was monitored at 254 nm. The selectivity (inter-assay C.V.<4%), sensitivity (limits of quantitation of 0.25 microg/ml for HYD, DEX and IND, 0.5 microg/ml for PB and 1 microg/ml for OPB, despite ...
Omphalocele in a foal. An omphalocele was detected at birth in a male Arabian foal. The mass contained small intestine and after releasing a constricting band at the body wall, the contents slipped easily back into the abdomen. On the outside was the hairless pink membrane. The interior, now empty of small intestine, contained the umbilical arteries and vein, and a large urachus that extended from the bladder to the opening at the extremity of the mass. Six days after corrective surgery the foal was sent home and remained healthy.
Genetic manipulation of equine arteritis virus using full-length cDNA clones: separation of overlapping genes and expression of a foreign epitope. Equine arteritis virus (EAV) is an enveloped, positive-stranded RNA virus belonging to the family Arteriviridae of the order Nidovirales. The unsegmented, infectious genome of EAV is 12,704 nt in length [exclusive of the poly(A) tail] and contains eight overlapping genes that are expressed from a 3'-coterminal nested set of seven leader-containing mRNAs. To investigate the importance of the overlapping gene arrangement in the viral life-cycle and to facilitate the genetic manipulation of the viral genome, a series of mutant full-length cDNA clones was constructed in which either EAV open readi...
Cross-species and intraspecies morphometric analysis of the corpus callosum. A cross-species morphometric study of the corpus callosum was performed in the rat, rabbit, cat, dog, horse, cow and human. Across species, the results indicate a strong, although less than linear, dependency of callosal size on brain weight. This relation tends to lose significance within species. This is consistent with other morphometric studies indicating a tendency to decrease the correlations between morphometric variables in within-species analyses as compared to between-species analyses. There are species differences in the relative size of some callosal segments particularly in the po...
In vitro effects of erythromycin, lidocaine, and metoclopramide on smooth muscle from the pyloric antrum, proximal portion of the duodenum, and middle portion of the jejunum of horses. To evaluate effects of erythromycin, lidocaine, and metoclopramide on smooth muscle of the pyloric antrum (PA), proximal portion of the duodenum (PD), and middle portion of the jejunum (MJ) of horses. Sample Population-Strips of smooth muscle from 7 horses. Methods: Isolated muscle strips were suspended in a bath and attached to isometric force transducers. Once stable spontaneous contractions were observed, agents were added. Isometric stress responses were compared with the amplitude of spontaneous contractions. Results: A single dose of erythromycin to the PA increased contractile amplitude...
Prokinetic effects of erythromycin on the ileum, cecum, and pelvic flexure of horses during the postoperative period. To evaluate the effect of erythromycin on motility of the ileum, cecum, and pelvic flexure of horses during the postoperative and post-recovery periods. Methods: 8 healthy adult horses. Methods: Horses were anesthetized and bipolar electrodes were implanted in smooth muscle of the ileum, cecum, and pelvic flexure. Approximately 4, 16, and 24 hours (postoperative recording sessions) and at least 8 days (post-recovery recording session) after surgery, myoelectric activity was recorded before and after administration of erythromycin (0.5 mg/kg). Results: Following erythromycin administration, myo...
Metabolic and mitogenic activities of insulin-like growth factor-1 in interleukin-1-conditioned equine cartilage. To determine response of interleukin-1alpha (IL-1alpha)-conditioned equine articular cartilage explants to insulin-like growth factor-1 (IGF-1). Sample Population-Cartilage from the trochlea and condyles of the femur of a clinically normal 4-year-old horse. Methods: Effects of IGF-1 (0 to 500 ng/ml) after addition of IL-1alpha were evaluated by assessing matrix responses, using a sulfated glycosaminoglycan (GAG) assay, matrix 35SO4 GAG incorporation, and release of GAG. Mitogenic response was assessed by 3H-thymidine incorporation into DNA and fluorometric assay of total DNA concentration. Res...
Use of specific sugars to inhibit bacterial adherence to equine endometrium in vitro. To determine whether specific sugars inhibit adhesion of Streptococcus zooepidemicus, Pseudomonas aeruginosa, and Escherichia coli to equine endometrial epithelial cells in vitro. Methods: Endometrial biopsy specimens collected during estrus from 7 healthy mares. Methods: Endometrial specimens on glass slides were incubated for 30 minutes at 4 C with suspensions of S. zooepidemicus, P. aeruginosa, or E. coli in phosphate-buffered saline solution (PBSS) alone or with various concentrations of D-(+)-mannose, N-acetyl-D-glucosamine, N-acetyl-D-galactosamine, D-(+)-glucose, galactose, or N-acetyl-...
Intracellular microelectrode recording to characterize inhibitory neuromuscular transmission in jejunum of horses. To evaluate electrical activity of jejunal circular muscle in horses and characterize electrical responses to stimulation by intrinsic inhibitory neurons. Methods: Portions of jejunum obtained from horses euthanatized for reasons other than gastrointestinal tract disease. Methods: Isolated circular muscle preparations were perfused with oxygenated modified Krebs solution. Glass microelectrodes were used for intracellular recording of membrane potentials from single smooth muscle cells. Electrical activity and responses to electrical field stimulation (EFS) of intrinsic neurons in the presence ...
Protein analysis of Babesia caballi merozoites by two-dimensional polyacrylamide gel electrophoresis and western blotting. Babesia caballi merozoites were prepared by combining two improved methods of cultivation and purification of merozoites using Percoll-gradiation, and the protein compositions of merozoites were analyzed by two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting. The relative molecular masses of the major proteins and protein masses separated by electrophoresis were >94, 80-70, 50-45, 34-30, 30-28 and 18 kDa. By Western blotting, twelve proteins or protein groups were recognized by pooled sera from two horses experimentally infected with B. caballi. Among...
Equine orbital neoplasia: a review of 10 cases (1983-1998). The clinical manifestations, laboratory findings, and survival times of 10 horses with orbital neoplasms are reported. In all cases, orbital neoplasms were malignant and locally invasive with no defined surgical circumscribed edges. It was often difficult to identify the primary cell type of the neoplasia in histologic specimens due to the poorly differentiated, anaplastic nature of the majority of cases. All except one horse were eventually euthanized 2 mo to 5 y after diagnosis due to poor response to treatment, metastasis, or unrelenting orbital neoplasia. Mean survival time increased with ...
Equine metabolism of buspirone studied by high-performance liquid chromatography/mass spectrometry. The metabolism and urinary excretion of a 100 mg dose of the non-sedating anxiolytic drug buspirone was examined using high-performance liquid chromatography/electrospray ionization mass spectrometry in the positive ion mode. In addition to a significant proportion of unchanged buspirone we were able to detect three major metabolite classes. These were identified as monohydroxy, dihydroxy and dihydroxymethoxy products. Detection of the metabolites and the parent drug was possible in all the urine samples collected (1-12 h) post-administration.
