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Topic:Veterinary Research
Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
Noniatrogenic rectal tears in three horses. Rectal tears were detected in three horses treated for colic. Based on historical, clinical, and postmortem findings, the tears could not be attributed to the attending veterinarian and were therefore not iatrogenic (physician induced). One tear was attributable to an infarction that presumably resulted from thromboembolism; 1 tear occurred without any evidence of external cause and resulted in such severe peritonitis that the cause and resulted in such severe peritonitis that the cause could not be determined, and 1 tear occurred during rectal palpation by the owner, before he called the vete...
Pulmonary function tests in standing ponies: reproducibility and effect of vagal blockade. Arterial blood gas tensions, pulmonary mechanics, and lung volumes were measured in 4 sedated ponies every hour for 6 hours and in 5 ponies 4 times at 2-month intervals to assess the short- and long-term reproducibility of pulmonary function measurements. Variability in blood gas tensions was small over the short- and long-term measurement periods, whereas the variability in total respiratory resistance and functional residual capacity was small over the short term but larger over the long term. The variability in tidal volume, minute ventilation, respiratory rate, and dynamic and quasistatic ...
Antigenic stimulation of T lymphocytes in chronic nononcogenic retrovirus infection: equine infectious anemia. Equine infectious anemia is a chronic disease of horses caused by a nononcogenic retrovirus. Studies were undertaken to determine the types of cells involved in the in vitro lymphoproliferative response to viral antigens and the dynamics of this reaction. It was observed that reactive lymphocytes were present at unpredictable times in the peripheral blood of infected horses. This reaction was shown to be specific for the interaction of equine infectious anemia virus and T lymphocytes. Enriched B-lymphocyte populations did not divide when exposed to equine infectious anemia virus. Macrophages w...
Radiographic characterization of diaphragmatic excursion in halothane-anesthetized ponies: spontaneous and controlled ventilation systems. A radiograph technique for identification of diaphragmatic segments and quantitation of their contribution to total diaphragmatic function was developed. five anesthetized ponies were studied on 3 separate occasions. Studies were made of the ponies in left lateral recumbency at 2 anesthetic levels (1 and 2 minimal alveolar anesthetic concentrations; halothane) and under spontaneous and controlled ventilation systems. General pattern of diaphragmatic displacement was unchanged by increased depth of anesthesia. Controlled ventilation altered the pattern of diaphragmatic displacement. Diaphragmat...
Effect of induced back pain on gait and performance of trotting horses. Back pain was induced in Standardbred horses by multiple intramuscular injections of a concentrated lactic acid solution into the left longissimus dorsi muscle. The investigation was divided into 2 parts. In Stage 1, 2 trotters were exercised on a treadmill and filmed by high speed cinematography before and after the induction of back pain. No signs of hindlimb lameness were evident and no quantitative changes in the components of the gait resulted, but a noticeable reduction was seen in performance capacity. Stage 2 involved a more intensive clinical and cinematic analysis of 3 horses. In the...
Chemotaxis of horse polymorphonuclear leukocytes to N-formyl-L-methionyl-L-leucyl-L-phenylalanine. Horse polymorphonuclear leukocytes (PMN) isolated from horse blood by sedimentation and isotonic lysis and having about 25% accompanying lymphocytes were as effective at chemotaxis as nearly pure PMN isolated by density gradient techniques. N-Formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP), used as a representative of the formylmethionyl peptides (produced by prokaryocytic organisms), was effective as a chemoattractant only at the high concentration of 10(-4) M. When serum was preincubated with FMLP at concentrations as low as 10(-8) M, the serum attracted horse PMN. This activity was not g...
Survey of equine hydatidosis in Great Britain. A survey of 1141 horses and ponies, for the presence of hydatid infection, was carried out at an export meat plant near Bristol. Lesions were attributed carefully by morphological, histological and immunological examinations and assessed by flame cell activity. All available detail concerning the animals was recorded. The surveys in the literature were assessed for the criteria on which their figures were based and were compared with the findings of this present survey. Attention was drawn to the apparent discord between the results of some surveys and others.
The concept of osmolality: its use in the evaluation of “dehydration” in the horse. Osmolality is an indication of the osmotic pressure of plasma and depends on the amount of solute and solvent (water) present. The mean (+sd) plasma osmolality of 100 clinically normal animals was 282 (+6) mOsm/kg using lithium heparin as anticoagulant. The equation, osmolality=1.86 (sodium + potassium) +glucose +blood urea nitrogen + 9, was found to predict only crudely plasma osmolality. The plasma sodium: osmolality ratio was 0.49. Water and electrolyte disorders are classified into 3 types based on the measurement of electrolytes and osmolality: (1) Hypertonic dehydration (true dehydration...
Isolation and characterization of beta- and gamma-caseins from horse milk. Three groups of casein components were isolated from horse milk. Group I is almost insoluble at acid and neutral pH, and is rather heterogeneous on alkaline gels with or without sodium dodecyl sulphate. Group II shows strong similarity to beta-casein from other species, as concluded from its amino acid composition and its N- and C-terminal sequences. This group consists of five electrophoretically distinguishable forms, all containing ester phosphate groups but no carbohydrate. Group III is composed of C-terminal fragments of the beta-like (group II) fraction and probably arises from the actio...
Assessment of the calcium and phosphorus nutrition in horses by analysis of urine. Studied were made to determine if a practical assessment of the calcium and phosphorus nutrition of horses could be obtained from an analysis of urine samples. The concentrations of Ca and P in urine samples changed markedly when groups of 4 mares were fed diets containing from 1.0 to 3.9 g Ca/kg and from 1.5 to 6.1 g P/kg, but serum concentrations of Ca and P remained relatively constant. The concentrations in single urine samples were considered unreliable indicators of excretion of the minerals because of variations in water excretion, and two methods to overcome this problem were examined....
The efficacy of ivermectin against Strongyloides westeri in foals. Seven foals naturally infected with Strongyloides westeri were injected intramuscularly with ivermectin at a dosage rate of 200 mcg per kg body weight. No adverse effects to treatment were observed. Weekly faecal egg counts showed a greater than 99 per cent reduction of S. westeri egg output compared with 7 untreated foals during the 21 days following treatment.
Deuteromethylation of dimethylxanthines: a gas chromatographic mass spectrometric method for confirmatory analysis in horse urine extracts. The methylated xanthines caffeine and/or theobromine are commonly encountered in drug-positive samples from racehorses and their metabolism and excretion in the horse and their analysis in urinary extracts has been of particular interest in this laboratory. Due to their polar nature the dimethylxanthines theobromine, theophylline and paraxanthine give unsatisfactory gas chromatographic performance and require derivatization prior to analysis by gas chromatography mass spectrometry. The present paper describes a simple deuteromethylation procedure to render the compounds amenable to analysis by...
A linkage group composed of three coat color genes and three serum protein loci in horses. The equine coat color genes chestnut (e) and roan (Rn) have been tested for linkage to 15 protein and blood group loci. Data showing close or fairly close linkage to the serum albumin locus (Al) and loose linkage to the serum esterase locus (Es) for both e and Rn are presented. This means that three coat color genes (To, e and Rn) and three serum protein loci (Al, Gc, and Es) are linked in the same linkage group. The gene order can tentatively be written Al, Gc, Rn, To-e-Es. The implications of the results for studies on coat color inheritance in horses are discussed. The possibility of using ...
Modifications of a force plate system for equine gait analysis. A force plate system for measurement of the vertical component of the force applied by a horse's limb on ground contact was modified. The modifications included use of steel supporting posts for improved durability and an additional strain gauge on each arm of the Wheatstone bridge for increased sensitivity and temperature compensation. Data from clinically normal horses are provided to indicate the performance obtained with these modifications of the force plate system.
Proopiolipomelanocortin peptides in normal pituitary, pituitary tumor, and plasma of normal and Cushing’s horses. Using RIAs for six regions within proopiolipomelanocortin (proOLMC), gel filtration, and electrophoresis, we studied pituitary peptides in a normal horse and one with Cushing's disease caused by a pars intermedia adenoma. Almost all immunoreactive (IR) ACTH (78%) was 4,500 mol wt (4.5K) ACTH in normal pars distalis, but it was almost 100% corticotropin-like intermediate lobe peptide (CLIP) in normal pars intermedia. alpha MSH and beta MSH were found mainly in pars intermedia: equal concentrations of the beta MSH precursors, beta-lipotropin (beta LPH) and gamma LPH, were found in pars distalis....
Retropulsion-propulsion in equine large colon. The circular and longitudinal muscle coats of equine "midcolon" were found to be directly electrically coupled. They appear to act in concert, in healthy animals, as a pacemaker in the area of the large colon pelvic flexure, for retropulsive-propulsive myoelectrical events. The retropulsive events keep the cecum and right ventral and left ventral divisions of the colon filled, imposing a delay time for fermentation of cellulose and for bacterial protein synthesis. Point-to-point involvement of adjacent colon sections was slowed by cooling the intestinal contents with no adverse clinical signs....
Chemotaxis of radiolabeled equine neutrophils. A method for the isolation of equine neutrophils was developed using metrizamide cushions. A purity of greater than 95% was routinely obtained with greater than 90% viability. These cells were radiolabeled and tested for their chemotactic response in Boyden chambers to zymosan-activated equine serum, the partially purified equine complement component C5a, and formyl-L-methionyl-L-leucyl-L-phenylalanine. The time and ionic requirements for chemotaxis of radiolabeled equine neutrophils were investigated and maximal movement was observed at 2 hours' incubation and 1.0 mM Ca and 0.5 mM Mg. Dinitro...
Controlled tests of fenbendazole against migrating Strongylus vulgaris in ponies. Sixteen pony foals were reared worm-free and inoculated with Strongylus vulgaris. On day 7 after inoculation, 12 ponies were given a fenbendazole 10% suspension at dose rate of 50 mg/kg of body weight by stomach tube. On day 8 after inoculation, 8 of these ponies were given the 2nd treatment of the anthelmintic and on day 9, 4 of these ponies were given the 3rd treatment. (The other 4 of the 16 ponies were given only tap water, as controls.) The ponies were necropsied at death or on day 28 after inoculation. Fenbendazole was effective in minimizing the appearance of clinical signs associated w...
Dexamethasone metabolism in the horse. Dexamethasone and a metabolite, 9-fluoro-16α-methyl-6β, 11β, 16β-trihydroxy-1, 4-androstadiene-3, 17-dione, were detected in the urine of horses injected parenterally with the parent drug. The structure of the metabolite was elucidated by thin-layer chromatography, infrared spectroscopy, mass spectroscopy and nuclear magnetic resonance spectroscopy.
Fluorimetric determination of unsubstituted and 9(8)-O-acetylated sialic acids in erythrocyte membranes. A method is described for all quantitative determination of free or glycosidically bound sialic acids with special reference to erythrocyte membranes. Sialic acids, unsubstituted in their side chains, quantitatively yield formaldehyde after mild periodate oxidation (1 mM NaIO4, 15 min, 4 degrees C, in the dark). The formaldehyde is determined by the reaction with acetylacetone and ammonium acetate which leads to a sensitive fluorogen (F 410/510 nm). Sialic acids O-acetylated at C-9 or C-8 are not oxidized under these conditions. Therefore, they can be determined quantitatively by measuring the...
Recovery of microorganisms from synovial and pleural fluids of animals using hyperosmolar media. L-phase (CWD) broth and plate media were used in parallel with conventional microbiological media during a 3-year period for culturing synovial and pleural fluids of animals. Two kinds of recoveries were obtained where parallel conventional methods were negative: (1) parent or normal bacteria, in very low numbers; and (2) Type B CWD variants in equally low numbers. Organisms in group 1 were: Streptococcus zooepidemicus from horses (2x); beta-hemolytic streptococci, Lancefield Gp. G (2x); Staphylococcus aureus; Actinobacillus, and Actinomyces viscosus. Group 2 consisted of Bacteroides sp., Prop...
Purification of horse eosinophil peroxidase. Eosinophil peroxidase (donor: hydrogen-peroxidase oxidoreductase, EC 1.11.1.7) was isolated in a highly purified form (415/280 nm ratio, 1.05) from horse peripheral blood eosinophil. Eosinophil peroxidase was extracted from intact eosinophils (98-100% purity) or isolated eosinophil granules with 0.05 M acetate buffer (pH 4.7)/0.18 M NaCl and purified by chromatography on Sephadex G-200 and carboxymethylcellulose. Final elution was with 0.05 M acetate buffer (pH 4.7)/ 1 M NaCl. Horse eosinophil peroxidase is a strongly basic protein with bacterial properties when combined with H2O2 and iodide, ...
