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Veterinary surgery : VS2006; 35(7); 601-610; doi: 10.1111/j.1532-950X.2006.00197.x

Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells: adipogenic and osteogenic capacity.

Abstract: To characterize equine bone marrow (BM)-derived mesenchymal stem cell (MSC) growth characteristics and frequency as well as their adipogenic and osteogenic differentiation potential. Methods: In vitro experimental study. Methods: Foals (n=3, age range, 17-51 days) and young horses (n=5, age range, 9 months to 5 years). Methods: Equine MSCs were harvested and isolated from sternal BM aspirates and grown up to passage 10 to determine cell-doubling (CD) characteristics. Limit dilution assays were performed on primary and passaged MSCs to determine the frequency of colony-forming units with a fibroblastic phenotype (CFU-F), and the frequency of MSC differentiation into adipocytes (CFU-Ad) and osteoblasts (CFU-Ob). Results: Initial MSC isolates had a lag phase with a significantly longer CD time (DT=4.9+/-1.6 days) compared with the average DT (1.4+/-0.22 days) of subsequent MSC passages. Approximately 1 in 4224+/-3265 of the total nucleated BM cells displayed fibroblast colony-forming activity. Primary MSCs differentiated in response to adipogenic and osteogenic inductive conditions and maintained their differentiation potential during subsequent passages. Conclusions: The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of foals and young adult horses are similar to those documented for BM MSCs of other mammalian species. Conclusions: The results have direct relevance to the use of BM as a potential source of adult stem cells for tissue engineering applications in equine veterinary medicine.
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Publication Date: 2006-10-10 PubMed ID: 17026544DOI: 10.1111/j.1532-950X.2006.00197.xGoogle Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

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This research article explores the growth characteristics, frequency, and potential for adipogenic and osteogenic differentiation of mesenchymal stem cells (MSCs) derived from equine bone marrow. The findings show a comparable frequency, in vitro growth rate, and differentiation potential in young horses and foals to that of other mammalian species, bringing relevance to the potential use of bone marrow as a source of adult stem cells in equine veterinary medicine.

Study Methodology

  • The study involved both foals (17 to 51 days old) and young horses (9 months to 5 years old).
  • MSCs were harvested and isolated from the animals’ sternal bone marrow and grown to observe cell-doubling characteristics up to passage 10.
  • Limit dilution assays were employed on both primary and passaged MSCs to determine the frequency of appearance of colony-forming units with a fibroblastic phenotype (CFU-F).
  • The study also evaluated the frequency of MSCs differentiating into these two specific cell types: adipocytes (CFU-Ad) and osteoblasts (CFU-Ob).

Findings

  • The initial MSC isolates exhibited a lag phase and a lengthy cell-doubling time compared to the average doubling time during subsequent MSCs passages.
  • Approximately 1 in 4224 of the total nucleated bone marrow cells showed fibroblast colony-forming activity, indicating the presence of MSCs with differentiation potential.
  • These primary MSCs proved capable of differentiating into adipocytes and osteoblasts under specific inductive conditions and maintained their differentiation potential during subsequent passages.

Conclusions

  • The frequency, in vitro growth rate, and adipogenic and osteogenic differentiation potential of MSCs in foals and young adult horses resemble those found in other mammalian species’ bone marrow MSCs.
  • This study emphasizes the suitability of using equine bone marrow as a potential source of adult stem cells for tissue engineering applications in the field of veterinary medicine, specifically for horses.

Cite This Article

APA
Vidal MA, Kilroy GE, Johnson JR, Lopez MJ, Moore RM, Gimble JM. (2006). Cell growth characteristics and differentiation frequency of adherent equine bone marrow-derived mesenchymal stromal cells: adipogenic and osteogenic capacity. Vet Surg, 35(7), 601-610. https://doi.org/10.1111/j.1532-950X.2006.00197.x

Publication

Veterinary surgery : VS
ISSN: 0161-3499
NlmUniqueID: 8113214
Country: United States
Language: English
Volume: 35
Issue: 7
Pages: 601-610

Researcher Affiliations

Vidal, Martin A
  • Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine and the Stem Cell Laboratory, Pennington Biomedical Research Center, Louisiana State University, Baton Rouge, LA, USA. mvidal@vetmed.lsu.edu
Kilroy, Gail E
    Johnson, Jill R
      Lopez, Mandi J
        Moore, Rustin M
          Gimble, Jeffrey M

            MeSH Terms

            • Adipocytes / cytology
            • Adipocytes / physiology
            • Adipogenesis / physiology
            • Animals
            • Cell Count / veterinary
            • Cell Culture Techniques / veterinary
            • Cell Differentiation / physiology
            • Cell Division / physiology
            • Colony-Forming Units Assay / veterinary
            • Horses
            • Kinetics
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / physiology
            • Osteoblasts / cytology
            • Osteoblasts / physiology
            • Osteogenesis / physiology

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