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Animals : an open access journal from MDPI2023; 13(8); 1352; doi: 10.3390/ani13081352

Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources.

Abstract: The investigation of multipotent stem/stromal cells (MSCs) in vitro represents an important basis for translational studies in large animal models. The study's aim was to examine and compare clinically relevant in vitro properties of equine MSCs, which were isolated from abdominal (abd), retrobulbar (rb) and subcutaneous (sc) adipose tissue by collagenase digestion (ASCs-) and an explant technique (ASCs-). Firstly, we examined proliferation and trilineage differentiation and, secondly, the cardiomyogenic differentiation potential using activin A, bone morphogenetic protein-4 and Dickkopf-1. Fibroblast-like, plastic-adherent ASCs- and ASCs- were obtained from all sources. The proliferation and chondrogenic differentiation potential did not differ significantly between the isolation methods and localizations. However, abd-ASCs- showed the highest adipogenic differentiation potential compared to rb- and sc-ASCs- on day 7 and abd-ASCs- a higher adipogenic potential compared to abd-ASCs- on day 14. Osteogenic differentiation potential was comparable at day 14, but by day 21, abd-ASCs- demonstrated a higher osteogenic potential compared to abd-ASCs- and rb-ASCs-. Cardiomyogenic differentiation could not be achieved. This study provides insight into the proliferation and multilineage differentiation potential of equine ASCs and is expected to provide a basis for future preclinical and clinical studies in horses.
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Publication Date: 2023-04-15 PubMed ID: 37106915PubMed Central: PMC10135324DOI: 10.3390/ani13081352Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the proliferation and differentiation potential of stem/stromal cells (MSCs) from different adipose tissue sources in horses with the intent of advancing preclinical and clinical studies.

Background of the Study

  • The study aims to understand and compare the properties of equine MSCs, which were isolated from different sources of adipose tissue—the abdomen, retrobulbar, and subcutaneously.
  • The cells were obtained through collagenase digestion and explant techniques.
  • The research interest in MSCs is significant as they possess tripotent characteristics, being capable of differentiation into adipose (fat), osteogenic (bone), and chondrogenic (cartilage) cells.

Methodology

  • The researchers first examined the proliferation and trilineage differentiation potential of the MSCs.
  • Following that, they attempted cardiomyogenic (heart) differentiation with the help of activin A, bone morphogenetic protein-4, and Dickkopf-1.
  • The MSCs examined were fibroblast-like and obtained from all possible sources.

Findings

  • The proliferation and chondrogenic differentiation potential remained fairly similar across different isolation techniques and origins.
  • However, MSCs from the abdominal source exhibited the highest adipogenic differentiation potential on day 7 as compared to those from retrobulbar and subcutaneous sources.
  • By day 14, abdominal MSCs showed higher adipogenic potential as compared to MSCs from the same location but isolated differently.
  • While osteogenic differentiation potential of the MSCs was more or less the same on day 14, by day 21, abdominal MSCs had a significantly higher osteogenic potential compared to those from other sources.
  • Attempts to achieve cardiomyogenic differentiation were unsuccessful.

Implications

  • This research adds to our knowledge about the proliferation and multilineage differentiation potential of equine MSCs, which is instrumental in conducting future preclinical and clinical studies on horses.
  • Understanding the potential of these cells can inflict major breakthroughs in translational studies in large animal models.
  • The limitation of the cells towards the generation of cardiac cells needs further research.

Cite This Article

APA
Stage HJ, Trappe S, Söllig K, Trachsel DS, Kirsch K, Zieger C, Merle R, Aschenbach JR, Gehlen H. (2023). Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources. Animals (Basel), 13(8), 1352. https://doi.org/10.3390/ani13081352

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 8
PII: 1352

Researcher Affiliations

Stage, Hannah J
  • Equine Clinic, Surgery and Radiology, Department of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
Trappe, Susanne
  • Institute of Veterinary Physiology, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
Söllig, Katharina
  • Institute of Veterinary Physiology, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
Trachsel, Dagmar S
  • Clinical Unit of Equine Internal Medicine, Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Veterinärplatz 1, 1210 Vienna, Austria.
Kirsch, Katharina
  • Institute of Veterinary Physiology, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
Zieger, Cornelia
  • Institute of Veterinary Pathology Department of Veterinary Medicine, Freie Universität Berlin, Robert-von-Ostertag-Straße 15, 14163 Berlin, Germany.
Merle, Roswitha
  • Institute for Veterinary Epidemiology and Biostatistics, Department of Veterinary Medicine, Freie Universität Berlin, Königsweg 67, 14163 Berlin, Germany.
Aschenbach, Jörg R
  • Institute of Veterinary Physiology, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.
Gehlen, Heidrun
  • Equine Clinic, Surgery and Radiology, Department of Veterinary Medicine, Freie Universität Berlin, Oertzenweg 19b, 14163 Berlin, Germany.

Grant Funding

  • 071045 / Wellcome Trust

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

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