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Home / Equine Research Bank / PLoS One / Strong stability and host specific bacterial community in fa...
PloS one2013; 8(9); e75079; doi: 10.1371/journal.pone.0075079

Strong stability and host specific bacterial community in faeces of ponies.

Abstract: The horse, as a hindgut fermenter, is reliant on its intestinal bacterial population for efficient diet utilisation. However, sudden disturbance of this population can result in severe colic or laminitis, both of which may require euthanasia. This study therefore aimed to determine the temporal stability of the bacterial population of faecal samples from six ponies maintained on a formulated high fibre diet. Bacterial 16S rRNA terminal restriction fragment length polymorphism (TRFLP) analyses of 10 faecal samples collected from 6 ponies at regular intervals over 72 hour trial periods identified a significant pony-specific profile (P<0.001) with strong stability. Within each pony, a significantly different population was found after 11 weeks on the same diet (P<0.001) and with greater intra-individual similarity. Total short chain fatty acid (SCFA) concentration increased in all ponies, but other changes (such as bacterial population diversity measures, individual major SCFA concentration) were significant and dependent on the individual. This study is the first to report the extent of stability of microbes resident in the intestinal tract as represented with such depth and frequency of faecal sampling. In doing so, this provides a baseline from which future trials can be planned and the extent to which results may be interpreted.
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Publication Date: 2013-09-11 PubMed ID: 24040388PubMed Central: PMC3770578DOI: 10.1371/journal.pone.0075079Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study evaluates the stability and host-specificity of the bacterial community in pony feces. It found a significant level of stability and diversity within each pony’s gut microbiome, even with changes in diet, and underscores the importance of this microbial community for equine health.

Objective of the Research

  • The main goal of this study was to analyze the stability of the bacterial population in fecal samples from ponies who were kept on a high-fiber diet. It was important to investigate this as horses, due to their physiological makeup as hindgut fermenters, rely on their intestinal bacterial population for effective digestion.
  • The research also focused on understanding the potentially severe impact of a sudden disruption of this bacterial population. A sudden change can result in distressing conditions like severe colic or laminitis, possibly even leading to the animal’s euthanasia.

Methodology of the Research

  • The researchers used bacterial 16S rRNA terminal restriction fragment length polymorphism (TRFLP) analyses on 10 faecal samples collected from six ponies.
  • The samples were collected at regular intervals over 72-hour trial periods.

Findings of the Research

  • The analyses identified a significant pony-specific bacterial profile in the fecal samples, with strong stability observed. This implies that each pony had a unique microbial composition which remained largely consistent over time.
  • Even after a period of 11 weeks on the same diet, a significantly different population was found within each pony, suggesting that the microbial make-up adapts and evolves over time based on the diet.
  • The total concentration of short chain fatty acids (SCFAs) increased in all ponies. However, other changes such as bacterial population diversity measures and individual major SCFA concentration were found to be significant and dependent on the individual.

Significant Implication to Future Studies

  • This research is groundbreaking as it is the first to report the extent of stability of microbes residing in the intestinal tract with this level of detail and frequency of faecal sampling.
  • This knowledge provides a fundamental baseline in planning future trials and interpreting the extent of the results from those trials.

Cite This Article

APA
Blackmore TM, Dugdale A, Argo CM, Curtis G, Pinloche E, Harris PA, Worgan HJ, Girdwood SE, Dougal K, Newbold CJ, McEwan NR. (2013). Strong stability and host specific bacterial community in faeces of ponies. PLoS One, 8(9), e75079. https://doi.org/10.1371/journal.pone.0075079

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 8
Issue: 9
Pages: e75079

Researcher Affiliations

Blackmore, Tina M
  • Institute of Biological, Environmental and Rural Sciences, Aberystwyth University, Aberystwyth, Ceredigion, Wales.
Dugdale, Alex
    Argo, Caroline McG
      Curtis, Gemma
        Pinloche, Eric
          Harris, Pat A
            Worgan, Hilary J
              Girdwood, Susan E
                Dougal, Kirsty
                  Newbold, C Jamie
                    McEwan, Neil R

                      MeSH Terms

                      • Animals
                      • DNA, Bacterial / genetics
                      • Fatty Acids, Volatile / metabolism
                      • Feces / microbiology
                      • Horses / microbiology
                      • Intestines / microbiology
                      • Metabolomics
                      • Microbiota
                      • Polymorphism, Restriction Fragment Length
                      • Principal Component Analysis
                      • RNA, Ribosomal, 16S / genetics
                      • Time Factors

                      Conflict of Interest Statement

                      Competing Interests: The trial was commercially funded by WALTHAM Centre For Pet Nutrition, of which one of the co-authors, Dr Pat Harris, is an employee. The ponies were fed Spillers(r) Happy Hoof for the duration of the trial (detailed in Methods), which is a marketed product of WALTHAM Centre For Pet Nutrition. There are no patents or products in development. This does not alter the authors\' adherence to all the PLOS ONE policies on sharing data and materials.

                      References

                      This article includes 41 references
                      1. Costa MC, Weese JS. The equine intestinal microbiome.. Animal Health Research Reviews 2012;13:121–128.
                        pubmed: 22626511
                      2. Shepherd ML, Swecker WS, Jensen RV, Ponder MA. Characterization of the fecal bacteria communities of forage-fed horses by pyrosequencing of 16S rRNA V4 gene amplicons.. FEMS Microbiol Lett 2011;326:62–68.
                        pubmed: 22092776
                      3. Dougal K, Harris PA, Edwards A, Pachebat JA, Blackmore TM. A comparison of the microbiome and metabolome of different regions of the equine hindgut.. FEMS Microbiology Ecology 2012;82:642–52.
                        pubmed: 22757649
                      4. Steelman SM, Chowdhary BP, Dowd S, Suchodulski J, Janecka JE. Pyrosequencing of 16S rRNA genes in fecal samples reveals high diversity of hindgut microflora in horses and potential links to chronic laminitis.. BMC Veterinary Research 2012;8:231.
                        pmc: PMC3538718pubmed: 23186268
                      5. Costa MC, Arroyo LG, Allen-Vercoe E, Stamphli HR, Kim PT. Comparison of the fecal microbiota of healthy horses and horses with colitis by high throughput sequencing of the V3–V5 region of the 16S rRNA gene.. PLOS One 2012;7:e41484.
                        pmc: PMC3409227pubmed: 22859989
                      6. Daly K, Stewart CS, Flint HJ, Shirazi-Beechey SP. Bacterial diversity within the equine large intestine as revealed by molecular analysis of cloned 16S rRNA genes.. FEMS Microbial Ecol 2001;38:141–151.
                      7. Daly K, Shirazi-Beechey SP. Design and evaluation of group-specific olignonucleotide probes for quantitative analysis of intestinal ecosystems: their application to assessment of equine colonic microflora.. FEMS Microbial Ecol 2003;44:243–252.
                        pubmed: 19719641
                      8. Yamano H, Koike S, Kobayashi Y, Hata H. Phylogenetic analysis of hindgut microbiota in Hokkaido native horses compared to light horses.. Animal Science Journal 2008;79:234–242.
                      9. Garner HE, Hutcheson DP, Coffman JR, Hahn AW. Lactic acidosis: a factor associated with equine laminitis.. Journal of Animal Science 1977;45:1037–1041.
                        pubmed: 599094
                      10. Milinovich GJ, Burrell PC, Pollitt CC, Klieve AV, Blackall LL. Microbial ecology of the equine hindgut during oligofructose-induced laminitis.. ISME Journal 2008;2:1089–1100.
                        pubmed: 18580970
                      11. Al Jassim RA, Andrews FM. The bacterial community of the horse gastrointestinal tract and its relation to fermentative acidosis, laminitis, colic and stomach ulcers.. Vet Clin North Am Equine Pract 2009;25:199–215.
                        pubmed: 19580934
                      12. Reece WO. Functional Anatomy and Physiology of Domestic Animals.. 2009;4th ed..
                      13. van Weyenberg S, Sales J, Janssens GPJ. Passage rate of digesta through the equine gastrointestinal tract: A review.. Livestock science 2006;99:3–12.
                      14. Liu W, Marsh TL, Cheng H, Forney LJ. Characterization of microbial diversity by determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA.. Applied and Environmental Microbiology 1997;63:4516–4522.
                        pmc: PMC168770pubmed: 9361437
                      15. de Fombelle A, Varloud M, Goachet A-G, Jacotot E, Philippeau C. Characterization of the microbial and biochemical profile of the different segments of the digestive tract in horses given two distinct diets.. Animal Sci. 2003;77:293–304.
                      16. Argenzio RA, Lowe JE, Pickard DW, Stevens CE. Digesta passage and water exchange in the equine large intestine.. American Journal of Physiology 1974;226:1035–1042.
                        pubmed: 4207578
                      17. Daly K, Proudman CJ, Duncan SH, Flint HJ, Dyer J. Alterations in microbiota and fermentation products in equine large intestine in response to dietary variation and intestinal disease.. Br J Nutr 2012;107:989–95.
                        pubmed: 21816118
                      18. Julliand V, de Fombelle A, Drogoul C, Jacotot E. Feeding and microbial disorders in horses: Part 3 – effects of three hay:grain ratios on microbial profile and activities.. J Equine Vet Sci 2001;21:543–546.
                      19. Parveen I, Moorby JM, Hirst WM, Morris SM, Fraser MD. Profiling of plasma and faeces by FT-IR to differentiate between heathland plant diets offered to zero-grazed sheep.. Animal Feed Science and Technology 2008;144:65–81.
                      20. Moore BE, Dehority BA. Effects of diet and hindgut defaunation on diet digestibility and microbial concentrations in the cecum and colon of the horse.. J. Anim Sci 1993;71:3350–3358.
                        pubmed: 8294287
                      21. Welkie DG, Stevenson DM, Weimer PJ. ARISA analysis of ruminal bacterial cimmunity dynamics in lactating dairy cows during the feeding cycle.. Anaerobe 2010;16:94–100.
                        pubmed: 19615457
                      22. Li M, Penner GB, Hernandez-Sanabria E, Oba M, Guan LL. Effects of sampling location and time, and host animal on assessment of bacterial diversity and fermentation parameters in the bovine rumen.. Journal of Applied Microbiology 2009;107:1924–1934.
                        pubmed: 19508296
                      23. Jami E, Mizrahi I. Composition and similarity of bovine rumen microbiota across individual animals.. PLOS One 2012;7:e33306.
                        pmc: PMC3303817pubmed: 22432013
                      24. Gronvold AM, L’Abbe-Lund TM, Strand E, Sorum H, Yannarell AC. Fecal microbiota of horses in the clinical setting: potential effects of penicillin and general anaesthesia.. Vet Microbiology 2010;145:366–372.
                        pubmed: 20434851
                      25. Willing B, Vörös A, Roos S, Jones C, Jansson A. Changes in faecal bacteria associated with concentrate and forage-only diets fed to horses in training.. Equine Veterinary Journal 2009;41:908–914.
                        pubmed: 20383990
                      26. Dougal K, Rand AS, Walsh CP, Newbold CJ. The effect of exercise on microbial activity in the hindgut of horses.. 2005;Proc. British Society of Animal Science Annual Conference, York. p. 47; 4–6 April.
                      27. Schoster A, Arroyo LG, Staemphfli HR, Weese JS. Comparison of microbial populations in te small intestine, large intestine and feces of healthy horses using terminal restriction fragment length polymorphism.. BMC Research Notes 2013;6:91.
                        pmc: PMC3622624pubmed: 23497580
                      28. Mackie RI, Wilkins CA. Enumeration of anaerobic bacterial microflora of the equine gastrointestinal tract.. Applied and Environmental Microbiology 1988;54:2155–2160.
                        pmc: PMC202828pubmed: 3190223
                      29. Varloud M, de Fombelle A, Goachet AG, Drogoul C, Julliand V. Partial and total apparent digestibility of dietary carbohydrates in horses as affected by the diet.. Anim Sci 2004;79:61–71.
                      30. Sadet-Bourgeteau S, Philippeau C, Faure C, Dequiedt S, Julliand V. Comparison of bacterial community structure between right ventral colon, caecum and feces by Automated Ribosomal Intergenic Spacer Analysis (ARISA).. Gut Microbiology: new insights into gut microbial ecosystems –7th Joint Symposium Rowett-INRA .
                      31. Zoetendal EG, Akkermans ADL, de Vos WM. Temperature gradient gel electrophoresis analysis from human fecal samples reveals stable and host-specific communities of active bacteria.. Applied and Environmental Microbiology 1998;64:3854–3859.
                        pmc: PMC106569pubmed: 9758810
                      32. Rajilic-Stojanovic M, Heilig HGHJ, Tims S, Zoetendal EG, de Vos WM. Long-term monitoring of the human intestinal microbiota composition.. Environmental Microbiology 2012;e-pub ahead of print.
                        pubmed: 23286720
                      33. Rajilic-Stojanovic M, Heilig HGHJ, Molenaar D, Kajander K, Surakka A. Development and application of the human intestinal tract chip, a phylogenetic microarray: analysis of universally conserved phylotypes in the abundant microbiota of young and elderly adults.. Environmental Microbiology 2009;11:1736–1751.
                        pmc: PMC2784037pubmed: 19508560
                      34. Dugdale AHA, Curtis GC, Harris PA, Argo CM. Assessment of body fat in the pony: Part I. Relationships between the anatomical distribution of adipose tissue, body composition and body condition.. Equine Veterinary Journal 2011;43:552–561.
                        pubmed: 21496091
                      35. Dugdale AHA, Curtis GC, Milne E, Harris PA, Argo CM. Assessment of body fat in the pony: Part II. Validation of the deuterium oxide dilution technique for the measurement of body fat.. Equine Veterinary Journal 2011;43:562–570.
                        pubmed: 21496088
                      36. Hongoh Y, Deevong P, Inoue T, Moriya S, Trakulnaleamsai S. Intra- and Interspecific comparisons of bacterial diversity and community structure support coevolution of gut microbiota and termite host.. Applied and Environmental Microbiology 2005;71:6590–6599.
                        pmc: PMC1287746pubmed: 16269686
                      37. Marsh TL, Saxman P, Cole J, Tiedje J. Terminal restriction fragment length polymorphism analysis program, a web-based research tool for microbial community analysis.. Applied and Environmental Microbiology 2000;66:3616–3620.
                        pmc: PMC92192pubmed: 10919828
                      38. Alvarado P, Manjon JL. Selection of enzymes for terminal restriction fragment length polymorphism analysis of fungal internally transcribed spacer sequences.. Applied and Environmental Microbiology 2009;75:4747–4752.
                        pmc: PMC2708443pubmed: 19465521
                      39. Felsenstein J. PHYLIP — Phylogeny Inference Package (Version 3.2).. Cladistics 1989;5:164–166.
                      40. Edwards A, Anesio AM, Rassner SM, Sattler B, Hubbard B. Possible interactions between bacterial diversity, microbial activity and supraglacial hydrology of cryoconite holes in Svalbard.. ISME Journal 2011;5:150–160.
                        pmc: PMC3105670pubmed: 20664552
                      41. Dunbar J, Ticknor LO, Kuske CR. Assessment of Microbial Diversity in Four Southwestern United States Soils by 16S rRNA Gene Terminal Restriction Fragment Analysis.. Applied and Environmental Microbiology 2000;66:2943–2950.
                        pmc: PMC92095pubmed: 10877790
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