Analyze Diet

Topic:Athletic Horses

The topic of athletic horses encompasses the study and analysis of horses that are trained and conditioned for performance in various disciplines such as racing, show jumping, dressage, and eventing. These horses are subject to specialized training regimens and nutritional plans to optimize their physical capabilities and performance outcomes. Research in this area often focuses on aspects such as biomechanics, cardiovascular fitness, muscle physiology, and injury prevention. This topic includes a collection of peer-reviewed research studies and scholarly articles that explore the physiological adaptations, training methodologies, and health management practices associated with maintaining and enhancing the performance of athletic horses.
Retrospective study of predictive variables for maximal heart rate (HRmax) in horses undergoing strenuous treadmill exercise.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 146-152 doi: 10.1111/j.2042-3306.2006.tb05531.x
Vincen TL, Newton JR, Deaton CM, Franklin SH, Biddick T, McKeever KH, McDonough P, Young LE, Hodgson DR, Marlin DJ.Heart rate is one of the most commonly measured variables in equine exercise physiology and relative exercise intensity commonly expressed as % of maximal heart rate. A number of influences affect maximal heart rate (HRmax), including age of the horse but other factors have not been described. Objective: To determine if fitness, health status, gender, breed, athletic use, body mass, in addition to age, are predictive of HRmax in the horse. Methods: Maximal heart rate data from 328 horses which underwent treadmill exercise tests at 5 different laboratories were obtained retrospectively. Univari...
Effects of draw reins on limb kinematics.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 452-456 doi: 10.1111/j.2042-3306.2006.tb05586.x
Byström A, Roepstorff L, Johnston C.No data exist on the GRF-kinematics relation due to changes caused by equestrian interventions. Objective: Through the judicious use of draw reins the rider can influence the kinematics of the horse to meet stated goals of dressage training. Relating the results to previously published kinetic data of the same experiment implies a possible relationship between kinetics and kinematics. Methods: The kinematics of 8 sound Swedish Warmblood horses were measured whilst the horses were being ridden with and without draw reins. Three conditions were evaluated: 1) draw reins only (DR), 2) combination ...
Effect of detraining on cardiorespiratory variables in young thoroughbred horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 210-213 doi: 10.1111/j.2042-3306.2006.tb05541.x
Mukai K, Ohmura H, Hiraga A, Eto D, Takahashi T, Asai Y, Jones JH.Thoroughbred racehorses often experience interruptions to their training. Identifying the effects of these changes and how they alter athletic performance might provide an insight on to how to prevent these changes from occurring. Objective: Training and detraining young Thoroughbreds alters their aerobic capacities with correlated changes in circulatory capacities; if horses remained spontaneously active in a pasture during their detraining period, their decreases in aerobic capacity during detraining would be reduced. Methods: We trained 6 Thoroughbred yearlings for 6 months using a conventi...
Effect of uphill exercise on equine superficial digital flexor tendon forces at trot and canter.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 435-439 doi: 10.1111/j.2042-3306.2006.tb05583.x
Takahashi T, Kasashima Y, Eto D, Mukai K, Hiraga A.One cause of overstrain injury to the superficial digital flexor tendon (SDFT) in horses is the force loaded on the SDFT during repeated running. Therefore, decreasing this force may reduce SDFT injury. It has been reported that strain on the SDFT decreases with a toe-wedge shoe. Uphill courses are used for training of racehorses, and the angle of hoof-sole to the horizon during uphill running is similar to that of the toe-wedge shoe. Objective: To determine the effects of uphill exercise on the force on the SDFT during trotting and cantering. Methods: Arthroscopically implantable force probes...
Use of a global positioning and heart rate monitoring system to assess training load in a group of thoroughbred racehorses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 106-109 doi: 10.1111/j.2042-3306.2006.tb05523.x
Kingston JK, Soppet GM, Rogers CW, Firth EC.Training is an important variable for determining athletic success. Nonetheless, there has been minimal scientific evaluation of racehorse training programmes. Training of racehorses focuses on running the horses at certain speeds using a combination of a stopwatch and rider's 'feel' for a horse's work intensity. Consequently, actual work intensity for individual horses is not clearly defined. Objective: To 1) utilise a combined global positioning system (GPS) and heart rate monitor system to quantify training intensity and physiological responses of a group of racehorses undergoing training a...
Synthesis of proglycogen and macroglycogen in skeletal muscle of standardbred trotters after intermittent exercise.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 335-339 doi: 10.1111/j.2042-3306.2006.tb05564.x
Bröjer J, Holm S, Jonasson R, Hedenström U, Essén-Gustavsson B.The degradation of glycogen and its two forms, proglycogen (PG) and macroglycogen (MG) has been studied in horses performing different types of exercise, but no information is available about the resynthesis of PG and MG after exercise. Objective: To determine the resynthesis of PG and MG in skeletal muscle after intermittent uphill exercise. Methods: At a training camp 9 well-trained Standardbred trotters performed a training session comprising a warm-up period, 7 repeated 500 m bouts of exercise on an uphill slope and a recovery period. Muscle biopsies (m. gluteus medius) for analysis of PG,...
Oral vitamin E supplementation on oxidative stress, vitamin and antioxidant status in intensely exercised horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 617-621 doi: 10.1111/j.2042-3306.2006.tb05614.x
Williams CA, Carlucci SA.Vitamin E is the most commonly supplemented antioxidant in horses; however, previous research is not conclusive as to the recommended level for exercising horses. Objective: To evaluate the effects of 3 levels of vitamin E supplementation on oxidative stress and vitamin/antioxidant status in intensely exercised horses to determine the optimal level of vitamin E supplementation. Methods: Twelve unfit Standardbreds were divided into 3 groups, supplemented orally with 0 (CON), 5000 (MOD), or 10,000 (HI) iu/day of DL-alpha-tocopheryl acetate. The 3 x 3 Latin square design consisted of three 4 week...
Energetic cost of locomotion on different equine treadmills.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 365-369 doi: 10.1111/j.2042-3306.2006.tb05570.x
Jones JH, Ohmura H, Stanley SD, Hiraga A.Human athletes run faster and experience fewer injuries when running on surfaces with a stiffness 'tuned' to their bodies. We questioned if the same might be true for horses, and if so, would running on surfaces of different stiffness cause a measurable change in the amount of energy required to move at a given speed? Objective: Different brands of commercial treadmills have pans of unequal stiffness, and this difference would result in different metabolic power requirements to locomote at a given speed. Methods: We tested for differences in stiffness between a Mustang 2200 and a Säto I comme...
Endurance exercise is associated with increased plasma cardiac troponin I in horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 27-31 doi: 10.1111/j.2042-3306.2006.tb05508.x
Holbrook TC, Birks EK, Sleeper MM, Durando M.Information is lacking regarding the influence of long distance exercise on the systemic concentration of cardiac troponin I (cTnI) in horses. Objective: To determine if the concentration of cTnI in horses competing in 80 and 160 km endurance races increases with exercise duration and if cTnI concentrations can be correlated with performance data. Methods: Blood samples for the measurement of cTnI and 3 min electrocardiogram recordings were obtained from horses prior to, during and after completion of 80 and 160 km endurance races at 3 ride sites during the 2004 and 2005 American Endurance Rid...
Cardiac arrhythmias during and after treadmill exercise in poorly performing thoroughbred racehorses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 163-170 doi: 10.1111/j.2042-3306.2006.tb05534.x
Jose-Cunilleras E, Young LE, Newton JR, Marlin DJ.The prevalence and severity of cardiac arrhythmias during exercise in athletic horses presented for poor performance is not well described. Objective: To describe prevalence and severity of ventricular and supraventricular arrhythmias immediately before, during and immediately after standardised incremental treadmill exercise tests (IET) to fatigue in Thoroughbred horses during investigation of poor performance. Methods: The electrocardiograms (ECG) of 88 Thoroughbred racehorses, judged to be free of significant heart disease or arrhythmia at rest, were used. A modified base-apex ECG was recor...
Effects of oral electrolyte supplementation on endurance horses competing in 80 km rides.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 19-26 doi: 10.1111/j.2042-3306.2006.tb05507.x
Sampieri F, Schott HC, Hinchcliff KW, Geor RJ, Jose-Cunilleras E.There is no evidence that use of oral electrolyte pastes enhances performance in competing endurance horses. Objective: To ascertain whether oral administration of a high dose (HD) of sodium chloride (NaCl) and potassium chloride (KCl) to endurance horses would differentially increase water intake, attenuate bodyweight (bwt) loss and improve performance when compared to a low dose (LD). Methods: A randomised, blinded, crossover study was conducted on 8 horses participating in two 80 km rides (same course, 28 days apart). Thirty minutes before and at 40 km of the first ride 4, horses received o...
Oxygen consumption (VO2) during trotting on a 10% decline.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 573-576 doi: 10.1111/j.2042-3306.2006.tb05607.x
Hoyt DF, Wickler SJ, Garcia SF.Although there have been reports of oxygen consumption measurements of horses running on the level and incline, there are no measurements during decline locomotion. This may be due, in part, to the potential for muscle damage produced by eccentric contractions. In man, running on a 10% decline, VO2 decreased by 35% and stride frequency (SF) decreased by 3% when compared to level locomotion. Objective: The rate of O2 consumption and SF would be decreased in horses on a 10% decline when compared to the level. Methods: Six horses (average 467 +/- 68 kg) were acclimated to trotting on the level an...
Cold air-induced late-phase bronchoconstriction in horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 535-539 doi: 10.1111/j.2042-3306.2006.tb05600.x
Davis MS, Royer CM, McKenzie EC, Williamson KK, Payton M, Marlin D.Inspired air is warmed to body temperature and fully humidified by the upper airway mucosa under normal resting conditions. This conditioning process may not be completed by the upper airways during conditions of increased minute ventilation or when the inspired air is unusually cold, resulting in cooling and desiccation of lower respiratory surfaces. Excess heat and water loss from intrapulmonary airways is believed to be the provocative stimulus for exercise-induced bronchoconstriction (occurring immediately after exercise) and associated late phase airway obstruction (occurring a few hours ...
Thyroid hormone responses to endurance exercise.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 32-36 doi: 10.1111/j.2042-3306.2006.tb05509.x
Graves EA, Schott HC, Marteniuk JV, Refsal KR, Nachreiner RF.Limited information exists about changes in circulating thyroid hormone concentrations during prolonged endurance exercise in horses. Objective: To examine the effects of prolonged exercise on serum iodothyronine concentrations in horses performing endurance exercise of varying distances. Methods: Serum concentrations of iodothyronines were measured in horses before and after completion of 40, 56, 80 and 160 km endurance rides (Study 1); daily during a 5 day, 424 km endurance ride (Study 2); and before and for 72 h after completion of a treadmill exercise test simulating a 60 km endurance ride...
Recruitment pattern of muscle fibre type during flat and sloped treadmill running in thoroughbred horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 349-353 doi: 10.1111/j.2042-3306.2006.tb05567.x
Eto D, Yamano S, Hiraga A, Miyata H.There is little information about the muscle fibre recruitment pattern during sloped and flat track running in Thoroughbred horses. Objective: To examine the glycogen depletion pattern of each muscle fibre type during running on a flat and sloped treadmill. Methods: Thirteen Thoroughbred horses (3-9 years old) were used. They were initially subjected to incremental exercise tests on a treadmill at 10 and 0% inclines in each horse to determine running speed at 90 and 60% VO2max. Needle biopsy samples were obtained from the middle gluteal muscle immediately after the running at 90% VO2max for 4 ...
Measurements of fitness in thoroughbred racehorses using field studies of heart rate and velocity with a global positioning system.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 113-117 doi: 10.1111/j.2042-3306.2006.tb05525.x
Vermeulen AD, Evans DL.Field based studies of changes in fitness are difficult to conduct in galloping Thoroughbreds. Measurements of heart rate (HR) and real time velocity with a global positioning system (GPS) could provide a method for routine field studies of fitness. Objective: To investigate measurements of fitness in the field without using multiple, standardised steps of increasing velocity in the exercise test. Methods: Twelve healthy 3- to 5-year-old Thoroughbreds were used in the study of reliability, and twelve 2-year-old Thoroughbreds in the investigation of the effect of training. Five second averages ...
Effects of a pre-moistened multilayered breathable fabric in promoting heat loss during recovery after exercise under hot conditions.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 303-307 doi: 10.1111/j.2042-3306.2006.tb05558.x
Foreman JH, Benson GJ, Foreman MH.Horses generate considerable internal heat burdens when exercising. Although common practice for a trainer or groom to place a wet blanket or towel on the dorsum of a hot horse post exercise, there are no data supporting the efficacy of this cooling method. Objective: To test the hypothesis that a pre-moistened blanket designed with a multilayered breathable fabric would enhance heat loss in horses post exercise. Methods: Eight treadmill-trained horses performed a standardised exercise test (SET) weekly for 3 weeks, with 3 different recovery treatments administered randomly. Pulmonary artery t...
Observations on respiratory flow strategies during and after intense treadmill exercise to fatigue in thoroughbred racehorses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 567-572 doi: 10.1111/j.2042-3306.2006.tb05606.x
Curtis RA, Kusano K, Evans DL.Locomotor-respiratory coupled (LRC) breath types are a feature of galloping exercise in horses. Differences in breath type have been demonstrated during exercise in particular the 'big respiratory cycle' (BRC) and 'flow hesitation'. To investigate breath types during recovery and quantitatively investigate BRCs during exercise to understanding the mechanism driving BRCs. Objective: To investigate the occurrence of different breath types during and after intense treadmill exercise, and test the hypothesis that large breaths (BRCs) were a function of respiratory frequency. Methods: Six trained a...
Optimal active recovery intensity in standardbreds after submaximal work.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 102-105 doi: 10.1111/j.2042-3306.2006.tb05522.x
Dahl S, Cotrel C, Leleu C.A retrospective study concerning spontaneous active recovery intensity, i.e. at a freely chosen speed, after a submaximal exercise in trotters showed that the mean intensity demanded by trainers corresponds to 40-50% of maximal heart rate (max HR; unpublished data). However, in human athletes, optimal active recovery intensity was found to be about 60-70% of max HR. Is the spontaneous recovery optimal after a submaximal exercise in trotters? Objective: To compare different recovery intensities and define the most efficient one. Methods: Thirty-seven trotters performed a standardised exercise t...
Exercise response, metabolism at rest and digestibility in athletic horses fed high-fat oats.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 626-630 doi: 10.1111/j.2042-3306.2006.tb05616.x
Lindberg JE, Essén-Gustavsson B, Dahlborn K, Gottlieb-Vedi M, Jansson A.High starch intakes increase the risk for metabolic disorders and therefore alternative feedstuffs are of interest. High-fat oat varieties have a lower starch and higher energy content than regular oats and may therefore be useful in this context. Objective: Feeding high fat oats causes no adverse effects on the response to exercise and that the total amount of oats offered could be reduced compared to feeding with regular oats. Methods: Twelve Standardbred trotters were fed regular oats (diet C), high-fat oats (F), and a mixture (50:50) of C and F (M), together with haylage (30:70), in a Lati...
Functional anatomy of the caudal thoracolumbar and lumbosacral spine in the horse.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 393-399 doi: 10.1111/j.2042-3306.2006.tb05575.x
Stubbs NC, Hodges PW, Jeffcott LB, Cowin G, Hodgson DR, McGowan CM.Research in spinal biomechanics and functional anatomy has advanced back pain research in man. Yet, despite the performance limiting nature of back pain in horses, there are few data for the equine spine. Objective: To describe aspects of functional anatomy of the equine thoracolumbar and lumbosacral (LS) spine and potential effects on performance. Methods: The first study investigated variations in LS vertebral formula by post mortem examination of 120 horses. Midline vertebral transection was carried out on 65 Thoroughbred (TB), 24 Standardbred (SB) and 31 other breeds. The second study inve...
Method for quantifying net anaerobic power in exercising horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 370-373 doi: 10.1111/j.2042-3306.2006.tb05571.x
Ohmura H, Hiraga A, Jones JH.There is no good method for measuring net anaerobic power in exercising horses to allow accurate estimates of total metabolic power. Objective: The increase in VO2max when breathing hyperoxic (HO) gas should be accompanied by a stoichiometrically equal (in terms of ATP turnover, i.e. energy equivalents) decrease in plasma lactate accumulation rate (Mlactate). Methods: Six 3-year-old Thoroughbreds were trained on an equine treadmill wearing a semi-open flow mask for measurement of VO2. After 4 months the horses ran with reproducible specific VO2max (VO2max/kg bwt). The mask design allowed mixin...
The effects of dietary N-3 and antioxidant supplementation on erythrocyte membrane fatty acid composition and fluidity in exercising horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 279-284 doi: 10.1111/j.2042-3306.2006.tb05553.x
Portier K, de Moffarts B, Fellman N, Kirschvink N, Motta C, Letellierw C, Ruelland A, van Erck E, Lekeux P, Couder J.Fatty acid supplementation could modulate erythrocyte membrane fluidity in horses at rest and during exercise, but information is lacking on the effect of exercise. Objective: To assess the effect of exercise with, and without, an oral antioxidant supplementation enriched with n-3 fatty acids on erythrocyte membrane fluidity (EMF) and fatty acid composition in eventing horses. Methods: Twelve healthy and regularly trained horses were divided randomly into 2 groups: group S received an oral antioxidant cocktail enriched in n-3 fatty acid (alphatocopherol, eicosapentaenoic acid [EPA] and docosah...
Associations between exercise-induced pulmonary haemorrhage, right ventricular dimensions and atrioventricular valve regurgitation in conditioned national hunt racehorses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 193-197 doi: 10.1111/j.2042-3306.2006.tb05538.x
Young LE, Helwegen MM, Rogers K, Kearns L, Schreiber C, Wood JL.Exercise-induced pulmonary haemorrhage (EIPH) and tricuspid (TR) and mitral valve regurgitation (MR) are conditions with high prevalence in conditioned Thoroughbreds. Stress failure of pulmonary capillaries, leading to EIPH, is most likely when pulmonary vascular pressure is increased, and mitral regurgitation can result in pulmonary venous hypertension. Objective: There might be an association between MR and EIPH and the right ventricle (RV) of horses known to suffer repeated episodes of EIPH, as their hearts would be subject to higher pulmonary vascular pressures and cardiac output during tr...
A nonsurgical method allowing continuous core temperature monitoring in mares for extended periods, including during endurance exercise.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 65-69 doi: 10.1111/j.2042-3306.2006.tb05515.x
Smith JE, Barnes AL, Maloney SK.Body temperature can be a useful measure of how well the horse responds to thermal challenges of exercise and environment. Currently there is little information available about how body temperature changes throughout an endurance ride, and no nonsurgical methods to evaluate internal temperature of exercising horses during prolonged periods and during long distances. Objective: To develop and test a nonsurgical method, using temperature loggers, of continuously recording internal temperature during exercise and over an extended period. Methods: Loggers were inserted transcervically into the ute...
Pre-exercise hypervolaemia is not detrimental to arterial oxygenation of horses performing a prolonged exercise protocol simulating the second day of a 3-day equestrian event.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 495-501 doi: 10.1111/j.2042-3306.2006.tb05594.x
Tennent-Brown BS, Goetz TE, Manohar M, Hassan AS, Freeman DE, Bundy JS, Evans MR.Hyperhydration, prior to prolonged moderate-intensity exercise simulating the 2nd day of a 3-day equestrian event (E3DEC), may induce arterial hypoxaemia detrimental to performance. Objective: Because moderate-intensity exercise does not induce arterial hypoxaemia in healthy horses, the effects of pre-exercise hypervolaemia on arterial oxygenation were examined during a prolonged exercise protocol. Methods: Blood-gas studies were carried out on 7 healthy, exercise-trained Thoroughbred horses in control and hyperhydration experiments. The study conformed to a randomised crossover design. The se...
Effect of corticosteroid and bronchodilator therapy on bronchoalveolar lavage cytology following intrapulmonary blood inoculation.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 516-522 doi: 10.1111/j.2042-3306.2006.tb05597.x
Walker HJ, Evans DL, Slocombe RF, Hodgson JL, Hodgson DR.Intrapulmonary blood causes an inflammatory response. Modifying this inflammatory response may be useful in the treatment of exercise induced pulmonary haemorrhage (EIPH). Objective: To evaluate the efficacy of 3 treatments, an oral bronchodilator, inhaled steroid and oral steroid in modifying the cellular response to intrapulmonary blood inoculation. Methods: Experimental design was a randomised block divided into 4 experimental segments of 2 weeks. Eight normal horses were randomly assigned to pairs and treatments. Autologous blood was inoculated into the lungs on 4 occasions over an 8 week ...
An echocardiographic and auscultation study of right heart responses to training in young national hunt thoroughbred horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 153-158 doi: 10.1111/j.2042-3306.2006.tb05532.x
Lightfoot G, Jose-Cunilleras E, Rogers K, Newton JR, Young LE.There are few data available to determine the effect of training on cardiac valve function. Objective: To investigate the effect of commercial race training on right ventricular (RV) and tricuspid valve function in an untrained group of National Hunt Thoroughbreds (TB). Methods: Cardiac auscultation, guided M-mode echocardiography of the RV, and colour flow Doppler (CFD) tricuspid valve and right atrium were performed in 90 TB horses (age 2-7 years) 1998-2003. Forty horses were examined at least once and 48 horses were examined on at least 2 occasions. Examinations were then classified as: i) ...
Does usefulness of potassium supplementation depend on speed?
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 74-79 doi: 10.1111/j.2042-3306.2006.tb05517.x
Hess TM, Kronfeld DS, Carter RA, Treiber KH, Byrd BM, Staniar WB, Smith LT, Gay LA, Harris PA.Electrolyte mixtures given to counter sweat loss usually contain abundant potassium. However, increases in plasma [K+] occur with exercise and supplementation may further increase plasma levels, potentially increasing the risk of neuromuscular hyperexcitability and development of adverse clinical sequellae. This proposition requires study. Objective: To compare effects of a K-rich electrolyte supplement (EM+K) to a K-free one (EM-K) on plasma [K+], [Ca++] and acid-base status during an endurance incremental exercise test on the treadmill. Methods: The test consisted of 3 bouts (simulating loop...
Hormone response to training and competition in athletic horses.
Equine veterinary journal. Supplement    April 4, 2007   Issue 36 274-278 doi: 10.1111/j.2042-3306.2006.tb05552.x
Cayado P, Muñoz-Escassi B, Domínguez C, Manley W, Olabarri B, Sánchez de la Muela M, Castejon F, Marañon G, Vara E.It is recognised that the amount of psychological stress that an animal encounters determines the degree of response of the hypothalamic-pituitary-adrenal (HPA) axis. In human athletes, the added emotive stress of competition is an important element in the adrenal response. The aim of this study was to examine the effect of show-jumping as well as dressage on stress levels by comparing horses' stress response at a horse show compared to their familiar home. Methods: Fifty-one horses involved in competition were used. EDTA blood samples were collected before exercise, upon arrived to the school...
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