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Topic:Clinical Findings

Clinical findings in horses encompass a range of observable signs and symptoms identified during veterinary examinations that contribute to diagnosing and managing equine health conditions. These findings can include physical observations, such as changes in behavior, posture, or gait, as well as physiological measurements like heart rate, respiratory rate, and temperature. Diagnostics may also involve laboratory tests, imaging, and other diagnostic procedures to assess organ function and detect abnormalities. Recognizing and interpreting clinical findings are essential components of veterinary practice, aiding in the identification of diseases, monitoring treatment progress, and guiding therapeutic interventions. This page brings together peer-reviewed research studies and scholarly articles that explore the methodologies, interpretations, and implications of clinical findings in the context of equine health care.
Retrospective analysis of oblique and straight distal sesamoidean ligament desmitis in 52 horses.
Equine veterinary journal    March 11, 2021   Volume 54, Issue 2 312-322 doi: 10.1111/evj.13438
Hawkins A, O'Leary L, Bolt D, Fiske-Jackson A, Berner D, Smith R.Injuries to the oblique (ODSL) or straight (SDSL) distal sesamoidean ligaments are a recognised cause of distal limb lameness in the horse. However, there are only limited publications addressing common diagnostic features and prognosis. Objective: (a) Report findings on ultrasonography and standing low-field magnetic resonance imaging (sMRI) in horses with ODSL or SDSL injury; (b) Identify clinical variables associated with lesion type and (c) Identify factors associated with return to soundness in horses with ODSL or SDSL injury. Methods: Retrospective case series. Methods: Horses with a pri...
Morphological variability of the atrioventricular valve cusps in the equine heart.
Equine veterinary journal    March 10, 2021   Volume 54, Issue 1 167-175 doi: 10.1111/evj.13434
De Silva M, Tagliavia C, Galiazzo G, Gifuni G, Caiazza M, Chiocchetti R, Grandis A.The morphometric studies of the atrioventricular valves are still limited in the horse. Objective: To investigate the anatomy of the atrioventricular valves in the horse, focusing on the morphometric features of the valvular leaflets and the tendinous cords. We hypothesised that accessory leaflets occur commonly and exist as independent structures in the atrioventricular valves of the horse. Methods: Descriptive anatomical study. Methods: Twenty normal hearts from slaughtered half-bred horses were used. The cardiac weight and circumference were recorded. The atrioventricular valves were expose...
Clinical features, treatment, and outcome of aural hematomas in horses: 7 cases (2008-2019).
Journal of the American Veterinary Medical Association    March 9, 2021   Volume 258, Issue 6 654-660 doi: 10.2460/javma.258.6.654
Boorman S, Boone LH, White A.To characterize the clinical features, treatment, and outcome of aural hematomas in horses. Methods: 7 horses with 1 or 2 aural hematomas (8 ears in total) treated at a veterinary teaching hospital in 2008 through 2019. Methods: Data retrieved from medical records included signalment, pertinent historical information, clinical signs, diagnostic procedures (including dermatologic assessment), and treatments. Case outcome was determined from documentation in the medical record or via telephone communication with owners or referring veterinarians. Results: 3 horses were presented after recurrence...
Subconjunctival enucleation with orbital implant placement in standing horses: 20 cases (2014-2017).
Journal of the American Veterinary Medical Association    March 9, 2021   Volume 258, Issue 6 661-667 doi: 10.2460/javma.258.6.661
Betbeze CM, Dray SM, Fontenot RL.To develop a protocol for subconjunctival enucleation and orbital implant placement in standing horses and to document short- and long-term complications, cosmesis, and client satisfaction. Methods: 20 horses with nonneoplastic ocular disease requiring enucleation. Methods: A standardized protocol of surgical suite cleaning, patient preparation, sedation, local nerve blocks, surgical procedure, and postoperative care was performed. Owners were required to provide follow-up information at 1 year after surgery during a phone questionnaire. Additionally, some owners provided follow-up information...
A retrospective multicenter study on the use of locking compression plates for scapulohumeral arthrodesis in small equids.
Veterinary surgery : VS    March 8, 2021   Volume 50, Issue 5 954-965 doi: 10.1111/vsu.13618
Somm F, Suárez Sánchez-Andrade J, Martens A, Zedler ST, Klopfenstein MD, Boswell JC, Bladon BM, Lischer CJ, Richardson DW, Auer JA, Fürst AE....To report on technical aspects and outcomes after scapulohumeral arthrodesis (SHA) with a locking compression plate (LCP) in small equids. Methods: Retrospective multicenter case series. Methods: Client-owned Shetland Ponies, Miniature Shetlands, and American Miniature Horses (n = 15). Methods: Inclusion criteria were completed SHA with an LCP to treat scapulohumeral osteoarthritis/subluxation and availability of postoperative radiographs. Contributing surgeons completed a questionnaire to collect data. All radiographs were reviewed for this study. Follow-up information was obtained via re-a...
Complications following equine sacroiliac region analgesia are uncommon: A study in 118 horses.
PloS one    March 2, 2021   Volume 16, Issue 3 e0247781 doi: 10.1371/journal.pone.0247781
Offord SCJ, Read RM, Pudney CJ, Bathe AP.Diagnosis of sacroiliac region pain is supported by a positive response to sacroiliac region analgesia (SIRA). Varying techniques have been described for SIRA; with clinician preference often dictating method. Potential complications following SIRA include ataxia and recumbency. No study has specifically evaluated the prevalence of complications. To describe the complication prevalence following SIRA in a referral clinic. Retrospective cohort study. Review of records from horses presented to two of the authors at Rossdales, Newmarket, between January 2014 and December 2018, that underwent SIRA...
Bronchoalveolar lavage fluid cytological findings in healthy Amiata donkeys.
Open veterinary journal    February 28, 2021   Volume 11, Issue 1 160-164 doi: 10.4314/ovj.v11i1.23
The approach to respiratory diseases in donkeys is similar to that for horses; nevertheless, Bronchoalveolar lavage fluid (BALF) and tracheal wash cytology in this species have been described only a few times in the literature. To describe BALF cytological findings in a cohort of 24 healthy Amiata donkeys. Bronchoalveolar lavage (BAL) was carried out via standing sedation with a large animal where the BAL catheter passed blindly through the nasal passage into the trachea. The total nucleated cell count of the BALF was found similar to that already described in healthy horses and donkeys. No di...
Multiple Simultaneous Proliferative Lesions on the Prepuce and Penis of a Gelding.
Journal of equine veterinary science    February 28, 2021   Volume 101 103426 doi: 10.1016/j.jevs.2021.103426
Veado HC, Silva AS, Fagundes JLA, Bittencourt AA, de Castro MB, Câmara ACL.Skin lesions are among the leading diseases in equids, and the urogenital region is considered the third most frequently affected body location with tumors and tumor-like skin lesions in horses. Tumor-like skin lesions or multiple tumors of different types in the equine prepuce and penis are a challenging clinical presentation leading to misdiagnosis and subsequent ineffective treatment. An 18-year-old 395-kg male mixed-breed gelding was referred for evaluation of preputial enlargement. Clinical examination revealed a 2.5 × 2.0 × 0.8 cm proliferative ulcerated lesion on the penile mucosa...
Syndromic clubfoot beyond arthrogryposis and myelomeningocele: orthopedic treatment with Ponseti method.
Revista espanola de cirugia ortopedica y traumatologia (English ed.)    February 26, 2021   Volume 65, Issue 3 180-185 doi: 10.1016/j.recot.2020.09.005
Ferrando Meseguer E, Roig Sánchez S, Pino Almero L, Romano Bataller A, Mínguez Rey MF.Talipes equinovarus or clubfoot is a congenital deformity of the foot with bone, muscle, and tendon involvement. It's one of the most frequent foot malformations in pediatric orthopedics. Although generally idiopathic, it may have a syndromic cause and be associated with musculoskeletal, neurological, or connective tissue conditions. The treatment of choice in idiopathic clubfoot is the Ponseti method based on manipulation and fixation with serial casts that seek progressive correction of the deformity. The Ponseti method effectiveness has been demonstrated in arthrogryposis and myelomeningoce...
Evaluation of electrocardiographic repolarization parameters after administration of trimethoprim-sulfadiazine, detomidine, or their combination in horses.
American journal of veterinary research    February 26, 2021   Volume 82, Issue 3 207-217 doi: 10.2460/ajvr.82.3.207
Trachsel DS, Calloe K, J Rgensen E, Lunddahl CS, Pedersen PJ, Kanters JRK, Klaerke DA, Buhl R.To determine whether administration of trimethoprim-sulfadiazine (TMS), detomidine (DET), or TMS plus DET would be associated with changes in ECG repolarization parameters in horses. Methods: 9 healthy adult horses. Methods: Each horse received 4 treatments in a blinded, randomized, crossover study design as follows: TMS, 16 to 24 mg/kg, IV; DET, 0.015 to 0.02 mg/kg, IV; TMS plus DET; and saline (0.9% NaCl) solution. Surface ECG traces were obtained over 24 hours, and repolarization parameters were measured at predefined time points after each treatment and compared with a 2-way ANOVA for repe...
Blood glucose and subcutaneous continuous glucose monitoring in critically ill horses: A pilot study.
PloS one    February 24, 2021   Volume 16, Issue 2 e0247561 doi: 10.1371/journal.pone.0247561
Vitale V, Berg LC, Larsen BB, Hannesdottir A, Dybdahl Thomsen P, Laursen SH, Verwilghen D, van Galen G.This pilot prospective study reports the feasibility, management and cost of the use of a continuous glucose monitoring (CGM) system in critically ill adult horses and foals. We compared the glucose measurements obtained by the CGM device with blood glucose (BG) concentrations. Neonatal foals (0-2 weeks of age) and adult horses (> 1 year old) admitted in the period of March-May 2016 with clinical and laboratory parameters compatible with systemic inflammatory response syndrome (SIRS) were included. Glucose concentration was monitored every 4 hours on blood samples with a point-of-care (POC) gl...
Incidence, recurrence, and outcome of postrace atrial fibrillation in Thoroughbred horses.
Journal of veterinary internal medicine    February 19, 2021   Volume 35, Issue 2 1111-1120 doi: 10.1111/jvim.16063
Nath LC, Elliott AD, Weir J, Curl P, Rosanowski SM, Franklin S.Atrial fibrillation (AF) impacts performance and horse and jockey safety. Understanding the outcomes of AF identified postrace will better inform regulatory policy. Objective: To investigate the outcomes after episodes of AF identified postrace and determine whether affected horses are at increased risk of additional episodes compared to the general racing population. Methods: Total of 4684 Thoroughbred racehorses. Methods: Race records for Thoroughbred horses racing in Hong Kong from 2007 to 2017 were reviewed. Horses that performed below expectation were examined by cardiac auscultation and ...
Equine simplified acute physiology score: Personalised medicine for the equine emergency patient.
The Veterinary record    February 19, 2021   Volume 189, Issue 5 e136 doi: 10.1002/vetr.136
de Barros AMC, Silva AFR, Zibordi M, Spagnolo JD, Corrêa RR, Belli CB, de Camargo MM.Scoring models are useful tools that guide the attending clinician in gauging the severity of disease evolution and in evaluating the efficacy of treatment. There are few tools available with this purpose for the non-human patient, including horses. We aimed (i) to adapt the simplified acute physiology score 3 (SAPS-3) model for the equine species, reaching a margin of accuracy greater than 75% in the calculation of the probability of survival/death and (ii) to build a decision tree that helps the attending veterinarian in assessment of the clinical evolution of the equine patient. Methods: Fr...
A composite absorbable implant used to treat subchondral bone cysts in 38 horses.
Equine veterinary journal    February 18, 2021   Volume 54, Issue 1 97-105 doi: 10.1111/evj.13428
Ravanetti P, Lechartier A, Hamon M, Zucca E.In the last 30 years, several treatments have been proposed to treat subchondral cystic lesions (SCLs) but there have been no randomised studies to compare different methods and there is no consensus as to the optimal treatment. Objective: To evaluate a biocompatible absorbable implant for the treatment of SCLs in young horses in different anatomical locations. Methods: Retrospective case series. Methods: Horses with SCLs were treated with debridement through a trans-cortical extra-articular approach and an absorbable implant was inserted in the cavity. Clinical and radiographic follow-up was...
Stress fracture of the palmar, distal cortex of the third metacarpal bone: A diagnostic challenge with a good prognosis.
Equine veterinary journal    February 17, 2021   Volume 54, Issue 1 74-81 doi: 10.1111/evj.13426
Shan R, Johnston AS, Rosanowski SM, O'Shea J, Riggs CM.Transverse stress fracture of the palmar cortex of the distal aspect of the third metacarpal bone (TSF PCD McIII) is poorly documented. Objective: To describe the typical signalment of this injury, the common clinical and radiological signs and prognosis. Methods: A retrospective hospital-based case series with follow-up of racing records. Methods: All cases of TSF PCD McIII diagnosed among racehorses in training at the Hong Kong Jockey Club between 2011 and 2019 were identified. Clinical records were reviewed and documented signs were recorded for each case. Findings from a predefined list of...
Evaluating the efficacy of surgical excision and topical dimethyl sulphoxide (DMSO) in the treatment of equine cutaneous pythiosis.
Iranian journal of veterinary research    February 16, 2021   Volume 21, Issue 4 301-307 
Atiba A, Ghazy A, Hamad MH.Cutaneous pythiosis in horses is a chronic ulcerative granulomatous disease caused by the oomycete Pythium insidiosum. Objective: The objective of the present study was to evaluate the response of cutaneous pythiosis in horses to surgical excision and topical dimethyl sulphoxide (DMSO). Methods: Thirty horses were presented clinically with pruritus, fistulae discharging serosanguineous fluid, and output kunkers in different body areas (limb, abdomen, neck, and face). The clinical diagnosis was confirmed by isolation of the causative agent and histopathology. All animals were treated by surgica...
Implications of the neuroanatomy of the equine thoracolumbar vertebral column with regional anaesthesia and complications following desmotomy of the interspinous ligament.
Equine veterinary journal    February 15, 2021   Volume 53, Issue 4 649-655 doi: 10.1111/evj.13402
Derham AM, Schumacher J, O' Leary JM, Kelly G, Hahn CN.Impinging/overriding dorsal spinous processes (DSPs) of the thoracolumbar vertebrae are a common cause of poor performance in horses. In the last five decades, numerous surgical treatments have been reported on, from transverse transection of the affected DSPs, and endoscopic resection of the affected DSPs, to transection of the interspinous ligament. Until recently, cosmetic outcomes have been reported as good to excellent in studies. However, a previously unreported complication of neurogenic atrophy of the contralateral epaxial muscle following desmotomy of the interspinous ligament has bee...
Incomplete Ileocecal Bypass for Ileal Pathology in Horses: 21 Cases (2012-2019).
Animals : an open access journal from MDPI    February 5, 2021   Volume 11, Issue 2 403 doi: 10.3390/ani11020403
Giusto G, Cerullo A, Labate F, Gandini M.Incomplete ileocecal bypass can be performed in cases in which an ileal disfunction is suspected but resection of the diseased ileum is not necessary. Objective: To describe the clinical findings, the surgical technique, and the outcome of 21 cases of colic with ileal pathologies that underwent an incomplete ileocecal bypass. Methods: Historical, clinical, and surgical features of cases diagnosed with pathologies involving the ileum or the ileocecal valve that underwent ileocecal anastomosis without ileal resection were retrieved. Clinical (heart rate, duration of symptoms, presence of reflux,...
Neural changes following equine-assisted therapy for posttraumatic stress disorder: A longitudinal multimodal imaging study.
Human brain mapping    February 5, 2021   Volume 42, Issue 6 1930-1939 doi: 10.1002/hbm.25360
Zhu X, Suarez-Jimenez B, Zilcha-Mano S, Lazarov A, Arnon S, Lowell AL, Bergman M, Ryba M, Hamilton AJ, Hamilton JF, Turner JB, Markowitz JC....While effective treatments for posttraumatic stress disorder (PTSD) exist, many individuals, including military personnel and veterans fail to respond to them. Equine-assisted therapy (EAT), a novel PTSD treatment, may complement existing PTSD interventions. This study employs longitudinal neuro-imaging, including structural magnetic resonance imaging (sMRI), resting state-fMRI (rs-fMRI), and diffusion tensor imaging (DTI), to determine mechanisms and predictors of EAT outcomes for PTSD. Nineteen veterans with PTSD completed eight weekly group sessions of EAT undergoing multimodal MRI assessme...
Towards an improved pain assessment in castrated horses using facial expressions (HGS) and circulating miRNAs.
The Veterinary record    February 2, 2021   Volume 188, Issue 9 e82 doi: 10.1002/vetr.82
Dalla Costa E, Dai F, Lecchi C, Ambrogi F, Lebelt D, Stucke D, Ravasio G, Ceciliani F, Minero M.Pain in horses is an emergent welfare concern, and its assessment represents a challenge for equine clinicians. This study aimed at improving pain assessment in horses through a convergent validation of existing tools: we investigated whether an effective analgesic treatment influences the horse grimace scale (HGS) and the concentration of specific circulating microRNAs (miRNAs). Eleven stallions underwent routine surgical castration under general anaesthesia. They were divided into two analgesic treatment groups: castration with the administration of preoperative flunixin and castration with ...
Isolation of equid alphaherpesvirus 3 from a horse in Iceland with equine coital exanthema.
Acta veterinaria Scandinavica    February 2, 2021   Volume 63, Issue 1 6 doi: 10.1186/s13028-021-00572-4
Thorsteinsdóttir L, Guðmundsson GÖ, Jensson H, Torsteinsdóttir S, Svansson V.Equine coital exanthema (ECE) caused by equid alphaherpesvirus 3 (EHV-3) is a contagious venereal disease. It is characterized by the formation of papules, vesicles, pustules and ulcers on the external genitals of both mares and stallions. The Icelandic horse is the only breed in Iceland and has lived isolated in the country for over 1000 years. Three types of equine herpesviruses (EHV) have been found in Iceland, EHV-4, EHV-2 and EHV-5, while EHV-1 has never been detected. Symptoms resembling ECE have previous been observed in horses in Iceland, arousing suspicion of EHV-3 infection, but thi...
Investigation of the effects of orally administered trazodone on intraocular pressure, pupil diameter, physical examination variables, and sedation level in healthy equids.
American journal of veterinary research    January 23, 2021   Volume 82, Issue 2 138-143 doi: 10.2460/ajvr.82.2.138
Moss AL, Hritz RL, Hector RC, Wotman KL.To investigate the effects of orally administered trazodone on intraocular pressure (IOP), pupil diameter measured in the vertical plane (ie, vertical pupil diameter [VPD]), selected physical examination variables, and sedation level in healthy equids. Methods: 7 horses and 1 pony. Methods: Food was withheld for 12 hours prior to drug administration. After baseline (time 0) sedation scoring, physical examination, and measurement of IOP and VPD, equids received 1 dose (approx 6 mg/kg) of trazodone orally. Examination and measurement procedures were repeated 0.5, 1, 2, 4, 8, 12, and 24 hours aft...
Surfactant protein D concentrations in serum and bronchoalveolar lavage fluid from young healthy horses on pasture and in a barn environment.
American journal of veterinary research    January 23, 2021   Volume 82, Issue 2 152-157 doi: 10.2460/ajvr.82.2.152
Daly AE, Deible CG, Lamb CE, Santacaterina SM, Marzolf NL, Page AE, Horohov DW, Wood PL, Werre SR, Chapman SE, Christmann U.To evaluate surfactant protein D (SP-D) concentrations in serum and bronchoalveolar lavage fluid (BALF) from young healthy horses on pasture or housed in a typical barn. Methods: 20 young healthy horses. Methods: Horses were randomly assigned to 1 of 2 groups (pasture, n = 10; barn, 10), and serum and BALF samples were collected for SP-D determination at baseline (all horses on pasture) and 2 weeks and 4 weeks after the barn group of horses was relocated from the pasture to the barn. Other evaluations included physical and tracheoscopic examinations. Findings were compared within and between g...
Clinical Factors Associated With Survival Outcomes in Starved Equids: A Retrospective Case Series.
Journal of equine veterinary science    January 19, 2021   Volume 101 103370 doi: 10.1016/j.jevs.2020.103370
Schneider LG, Cox Self A, Hines MT, Lin-Zambito Ivey J.Each year in the United States, unwanted horses may become neglected, starved, or abandoned. Recovery therapies include refeeding and veterinary care, often requiring substantial time and financial investments. To better understand the likelihood for starved horses to successfully survive the first 100 days after initial evaluation, a retrospective case series was performed using hospital records of starved horses at the University of Tennessee College of Veterinary Medicine. A body condition score (BCS) of 3 or less and a malnourished diagnosis were utilized to select case records from an 11...
Effects of Leaving Amputated Ovaries Intra-Abdominally during Elective Bilateral Standing Laparoscopic Ovariectomy in Equids.
Animals : an open access journal from MDPI    January 18, 2021   Volume 11, Issue 1 232 doi: 10.3390/ani11010232
Devick IF, Hendrickson DA.There is risk of dropping an amputated ovary within the abdomen during standing laparoscopic ovariectomies in mares. If the ovary can no longer be directly visualized with the laparoscope, the procedure is then converted to a flank laparotomy for manual retrieval of the ovary which negates the minimally invasive nature of the laparoscopic procedure. The objective was to identify if ovaries left in the abdomen after amputation would atrophy or if they re-vascularize. Standing bilateral ovariectomies were performed in mature mares, but after transection of the ovarian pedicle, the ovaries were i...
Clinical Observations After Prebreeding Intrauterine Plasma Infusion in 18 Mares Inseminated With Thawed Frozen Semen.
Journal of equine veterinary science    January 16, 2021   Volume 99 103389 doi: 10.1016/j.jevs.2021.103389
Pasch L, Schmidt A, King W.The use of autologous blood processing tools including platelet-rich plasma (PRP) devices is increasingly widespread in veterinary medicine. In equine reproduction, a number of studies have explored the effects of intrauterine infusion of PRP on persistent mating-induced endometritis. Artificial insemination with thawed frozen semen incites an intrauterine inflammatory response and we sought to extend the applications of intrauterine PRP to normal mares being inseminated with frozen semen. We investigated a subset of our normal breeding population to observe the clinical effects of prebreeding...
Surgical Repair of a Unicortical Condylar Fracture in a Thoroughbred Racehorse Using a Bioabsorbable Screw.
Journal of equine veterinary science    January 16, 2021   Volume 99 103384 doi: 10.1016/j.jevs.2021.103384
Ravanetti P, Hamon M, Lechartier A, Zucca E.Surgical repair of unicortical fractures of the distal condyles of the third metacarpal/metatarsal bones (MCIII/MTIII) has been described. In the present case, a bioabsorbable screw was used to stabilize a unicortical fracture in the palmar aspect of the medial condyle of MCIII in a National Hunt Thoroughbred racehorse. The clinical, radiographic, and magnetic resonance imaging findings before and after surgery were recorded. Eighteen weeks after surgery, the lameness had resolved and the bone surrounding the fracture had recovered its normal architecture. One year after surgery, the horse was...
Assessment of clinical and microbiota responses to fecal microbial transplantation in adult horses with diarrhea.
PloS one    January 14, 2021   Volume 16, Issue 1 e0244381 doi: 10.1371/journal.pone.0244381
McKinney CA, Bedenice D, Pacheco AP, Oliveira BCM, Paradis MR, Mazan M, Widmer G.Fecal microbial transplantation (FMT) is empirically implemented in horses with colitis to facilitate resolution of diarrhea. The purpose of this study was to assess FMT as a clinical treatment and modulator of fecal microbiota in hospitalized horses with colitis. A total of 22 horses with moderate to severe diarrhea, consistent with a diagnosis of colitis, were enrolled at two referral hospitals (L1: n = 12; L2: n = 10). FMT was performed in all 12 patients on 3 consecutive days at L1, while treatment at L2 consisted of standard care without FMT. Manure was collected once daily for 4 days fro...
Evaluation of Overground Endoscopy Findings in Colombian Criollo Paso Horses.
Journal of equine veterinary science    January 12, 2021   Volume 99 103374 doi: 10.1016/j.jevs.2021.103374
Joó K, Duque Betancourt D, Vasquez Marin T, Parra Moyano LA.The dynamic upper airway functional (URT) problems of Colombian paso horses (CPH) have not been investigated in literature up to date. These horses perform special walking gaits with high poll flexion of the neck. Our goal is to evaluate the upper airway mechanics in CPH, showing abnormal respiratory sounds and poor performance during exercise. Resting and overground endoscopy was performed in 40 CPHs. Statistical analyses were performed using the sciPy package. One-tailed Fisher exact tests were used to check for positive contingency between each pair of upper respiratory tract disorder (P < ...
Successful vitrification of manually punctured equine embryos.
Equine veterinary journal    January 11, 2021   Volume 53, Issue 6 1227-1233 doi: 10.1111/evj.13400
Wilsher S, Rigali F, Kovacsy S, Allen WT.Successful vitrification of equine expanded blastocysts requires collapse of the blastocoele cavity using a micromanipulator-mounted biopsy pipette on an inverted microscope. Such equipment is expensive and requires user skill. Objective: To develop a manual method of blastocoele collapse prior to vitrification using commercial products. Methods: In vivo experiment. Methods: Seventy-nine Day 7 or 8 embryos were measured and graded. Twenty were vitrified following micromanipulator-assisted puncture and aspiration before being used to validate commercial human vitrification and warming kits cont...
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