Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Pietrani M, Losinno L, Cuervo Arango J.In the present study, 2.228 cycles of 180 Polo Argentino donor mares from an embryo transfer program in Argentina were examined to evaluate the effects of: (1) Interval from Prostaglandin F2alpha analog treatment to ovulation (ITO) on embryo recovery rate (ERR); (2) ITO on number of embryos per flushing (EPF); (3) ITO on multiple ovulation (MO) rate; (4) ITO from donor mare on pregnancy rate (PR) in recipient mares. Mares were inseminated with fresh semen from 31 fertile stallions in the induced estrus. Embryo flushing was performed 7-8 days postovulation. Following embryo flushing, donor mar...
Smith RM, Bhoora RV, Kotzé A, Grobler JP, Lee Dalton D.Translocation of animals in fragmented habitats is an important means of dispersal and gene flow, however, the movement of animals has led to the spread of various diseases globally and wildlife are often the reservoirs of these diseases. Currently, Cape mountain zebra are translocated within South Africa as a management method for augmentation of isolated and fragmented populations. The movement of pathogens due to translocations in local regions have gone largely unchecked, particularly where there may still be isolated regions that can be negatively affected. Equine piroplasmosis is a tick-...
Jaqueth AL, Turner TR, Iwaniuk ME, McIntosh BJ, Burk AO.Unlike traditional forage grasses, turfgrasses, which were developed to be tolerant of foot traffic and close mowing, may be suitable as alternative ground cover in areas of high hoof traffic such as dry lots. The objective of this study was to evaluate the potential of eight cool-season turfgrasses as alternative ground cover in heavy use areas. Cultivars were established via seeding in four replicated plots. To simulate horse traffic at a trot, a Baldree traffic simulator was driven over a section of the plot, either 0 (CON), 1 (LOW), or 2 (HIGH) times per week for 6 weeks followed by 4 week...
Wolford AN, Coverdale JA, Leatherwood JL, Pinchak WE, Anderson RC, Wickersham TA.Eight previously cecally cannulated Quarter Horse geldings were utilized in a crossover design with two 28-d periods with a 21-d washout period between to evaluate the influence of housing on the cecal environment and dry matter intake (DMI). Horses were adapted to diet and housing from day 1 to 19, DMI was determined from day 20 to 24, and cecal fluid was collected on day 28. Horses were paired by age and body weight (BW) and randomly assigned to treatment. Treatments consisted of housing horses individually in stalls or group housed in a pen. Regardless of treatment, all horses were individu...
Desantis S, Accogli G, Albrizio M, Rossi R, Cremonesi F, Lange Consiglio A.Equine amniotic mesenchymal cells (eAMCs) are involved in many mechanisms in tissue regenerative processes. Their secreted vesicles are important effectors in a wide array of biological processes, and contribute to in vivo healing of equine tendon lesions and endometrial inflammation. Glycoconjugates are involved in cellular recognition and in the efficient uptake of extracellular vesicles (EVs) by recipient cells. In this study, we evaluated the surface glycosylation pattern of eAMCs and their EVs from the eAMCs released in conditioned medium. We used a microarray procedure in which eAMCs and...
Holl HM, Pflug KM, Yates KM, Hoefs-Martin K, Shepard C, Cook DG, Lafayette C, Brooks SA.Variations in the SLC45A2 gene are responsible for the dilution phenotypes cream and pearl in domestic horses. Cream dilution is inherited in an incomplete dominant manner, diluting only red in the heterozygous state but both red and black pigments when two alleles are present. The pearl dilution is recessive and dilutes only the red and black pigment in the homozygous state or when paired with a cream allele. Horses that inherit one copy of pearl (C ) and one copy of the dominant cream allele (C ) display a dilution phenotype similar to that of homozygous cream, suggesting that pearl is the ...
Seo MG, Kwon OD, Kwak D.A 23-year-old male Thoroughbred horse at the Korean Military Academy appeared thin with visible rib bones and presented clinical signs of fever, anorexia, lethargy, and severe dehydration. To determine the presence of various febrile disease-causing agents, the 23 cohabiting horses at the academy, including this horse, were subjected to hematology, blood chemistry, and molecular analysis using whole blood samples collected during regular medical check-ups. On the basis of clinical history, physical examination, hematology, blood chemistry, and fecal examination, differential diagnosis using mo...
Safryghin A, Hebesberger DV, Wascher CAF.In a number of species, consistent behavioral differences between individuals have been described in standardized tests, e.g., novel object, open field test. Different behavioral expressions are reflective of different coping strategies of individuals in stressful situations. A causal link between behavioral responses and the activation of the physiological stress response is assumed but not thoroughly studied. Also, most standard paradigms investigating individual behavioral differences are framed in a fearful context, therefore the present study aimed to add a test in a more positive context...
Fortier Guillaume J, Laroche D, Babault N.The objective of this study was to identify the key biomechanical patterns (functional muscles and kinematics) of amateur horse riders during various cross-country jumps in equestrian. Eleven riders first performed a control condition that corresponded to jumps over three different obstacles (log wall, brush and tree trunk) before jumping over the same three obstacles in a cross-country course. 3D Kinematics and electromyographic (EMG) activity was synchronously collected which included seven muscles of the riders back, lower and upper limbs. Maximum voluntary isometric strength of knee extens...
Arévalo-Turrubiarte M, Olmeo C, Accornero P, Baratta M, Martignani E.Mesenchymal stem cells (MSCs) have been used in equines as an alternative therapy. A comparative study about the phenotype and in vitro performance of different MSCs tissue sources in adult equines was needed. This study might serve to provide the knowledge to select a valuable harvesting source of MSCs. Bone marrow, synovial and adipose (mesenteric, neck and tail fat) tissues were collected from adult equines. Cell surface markers expression (CD11α/CD18, CD45, CD79α, CD90, CD105 and MHC II) and in vitro differentiation assays were made. In vitro cell migration, cell growth and wound healing...
Brasileiro LS, Segabinazzi LGTM, Menezes E, Salgueiro CC, Novello G, Scheeren VFDC, Alvarenga MA, Nunes JF.The aim of this study was to evaluate the effect of coconut water as a component of extender in different formulations for cooling equine sperm. One ejaculate of fourteen stallions was collected. Sperm was diluted to 50 × 10 sperm/mL using five different extenders: ACP-105: powdered coconut water extender (ACP-105, ACP Biotecnologia, Brazil); ACP-Milk: ACP-105 + 20 g/L of skimmed milk; ACP-EY 2.5%: ACP-105 + 2.5% of egg yolk; ACP-EY 5%: ACP-105 + 5% of egg yolk; and BotuSêmen (Botupharma, Botucatu, Brazil) and cooled in passive cooling device (BotuFlex, Botupharma, Botucatu, Brazil) at 5...
Felkel S, Vogl C, Rigler D, Dobretsberger V, Chowdhary BP, Distl O, Fries R, Jagannathan V, Janečka JE, Leeb T, Lindgren G, McCue M, Metzger J....Analysis of the Y chromosome is the best-established way to reconstruct paternal family history in humans. Here, we applied fine-scaled Y-chromosomal haplotyping in horses with biallelic markers and demonstrate the potential of our approach to address the ancestry of sire lines. We de novo assembled a draft reference of the male-specific region of the Y chromosome from Illumina short reads and then screened 5.8 million basepairs for variants in 130 specimens from intensively selected and rural breeds and nine Przewalski's horses. Among domestic horses we confirmed the predominance of a young'c...
Mattacola CG, Quintana C, Crots J, Tumlin KI, Bonin S. During thoroughbred races, jockeys are placed in potentially injurious situations, often with inadequate safety equipment. Jockeys frequently sustain head injuries; therefore, it is important that they wear appropriately certified helmets. The goals of this study are (1) to perform impact attenuation testing according to ASTM F1163-15 on a sample of equestrian helmets commonly used by jockeys in the United States and (2) to quantify headform acceleration and residual crush after repeat impacts at the same location. Seven helmet models underwent impact attenuation testing according to ASTM...
Rowland M, Coombs T, Connor M.Traveller horses are often perceived to be exposed to poor welfare due to Travellers' traditional way of horsemanship. However, few studies have investigated Traveller horse welfare. Hence, the present study aims to explore Traveller horse owners' attitudes to horse care and welfare. Semi-structured interviews and discussion groups examined 14 Irish Traveller horse owners' attitudes and approach to horse ownership. Additionally, a body condition scoring (BCS) instrument was assessed for its accuracy and ease of use when applied by Traveller horse owners. Additionally, the BCS system was used t...
Shah SZA, Nawaz Z, Nawaz S, Carder G, Ali M, Soomro N, Compston PC.Equine ownership is a common income-generating strategy in Pakistan. In Karachi, donkey carts are used to transport building materials, commercial produce and garbage. This study aimed to articulate the role and welfare of donkeys used in waste management. We conducted interviews with donkey owners ( = 200), households which use donkey carts for waste collection ( = 50) and key informants ( = 14). To assess the welfare of donkeys, the Standardised Equine-Based Welfare Assessment Tool (SEBWAT) was used ( = 204). Collection of waste was the primary source of income for 89% of owners interviewed....
Ducheyne KD, Rizzo M, Daels PF, Stout TAE, de Ruijter-Villani M.Vitrified-warmed immature equine oocytes are able to complete the first meiotic division, but their subsequent developmental competence is compromised. Therefore, the present study investigated the effects of vitrifying immature horse oocytes on the chromosome and spindle configuration after IVM. Cumulus-oocytes complexes (COCs) were collected and divided into two groups based on mare age (young ≤14 years; old ≥16 years). COCs were then either directly matured invitro or vitrified and warmed before IVM. Spindle morphology and chromosome alignment within MII stage oocytes were assessed usin...
Sales F, Ferreira-Silva J, Vieira J, Basto S, Filho P, Rocha J, Moura MT, Oliveira M.Post-diluents could potentially increase semen cryotolerance, but remain poorly explored in horses. The aim was to evaluate the efficiency of post-diluents on frozen-thawed semen viability of two stallions (S1-S2). The cryopreserved semen was thawed at 50°C for 40 seconds. Semen motility and acrosin activity (AA) were determined during the thermo-resistance test (TRT). Progressive motility of S2 semen decreased after 60 and 90 minutes of TRT (TRT60 and TRT90) on the control compared to both post-diluents. The total motility of both S1 and S2 decreased on TRT60 and TRT90 semen control versus b...
Martins HS, Martins-Filho OA, Araujo MS, Martins NR, Lagares MA.Frozen equine semen has lower fertility compared to cooled semen. Due to the difficulty to obtain equine oocytes, a heterologous zona pellucida binding assay (ZBA) is an alternative method to predict the fertilizing capability of equine frozen sperm. The rate of capacitated and hyperactivated sperm according to their motility characteristics were analyzed with a Computer Assisted Sperm Analyzer. We believe this report describes for the first time the in vitro hyperactivation induction and the heterologous ZBA to predict the fertilizing ability of frozen equine sperm. Objective: This work aimed...
Sevane N, Sanz CR, Dunner S.Four loci seem responsible for the dilution of the basic coat colours in horse: Dun (D), Silver Dapple (Z), Champagne (CH) and Cream (C). Apart from the current phenotypes ascribed to these loci, pearl has been described as yet another diluted coat colour in this species. To date, this coat colour seems to segregate only in the Iberian breeds Purebred Spanish horse and Lusitano and has also been described in breeds of Iberian origin, such as Quarter Horses and Paint Horse, where it is referred to as the 'Barlink Factor'. This phenotype segregates in an autosomal recessive manner and resembles ...
Self Davies ZT, Spence AJ, Wilson AM.Horse locomotion is remarkably economical. Here, we measure external mechanical work of the galloping horse and relate it to published measurements of metabolic cost. Seven Thoroughbred horses were galloped (ridden) over force plates, under a racing surface. Twenty-six full strides of force data were recorded and used to calculate the external mechanical work of galloping. The mean sum of decrements of mechanical energy was -876 J (±280 J) per stride and increments were 2163 J (±538 J) per stride as horses were accelerating. Combination with published values for internal work and metabolic c...
Putnová L, Štohl R.Considering the extensive data sets and statistical techniques, animal breeding embodies a branch of machine learning that has a constantly increasing impact on breeding. In our study, information regarding the potential of machine learning and data mining within a large set of horses and breeds is presented. The individual assignment methods and factors influencing the success rate of the procedure are compared at the Czech population scale. The fixation index values ranged from 0.057 (HMS1) to 0.144 (HTG6), and the overall genetic differentiation amounted to 8.9% among the breeds. The highes...
Cuervo-Arango J, Claes AN, Stout TA.Advancing maternal age is known to negatively affect fertility in the horse. This age-related decrease in fertility has been linked primarily to reduced oocyte quality rather than to impaired uterine function. In the past decade, the use of ovum pick-up (OPU) and ICSI to produce foals has rapidly gaining popularity amongst sport horse breeders. However, it is not yet known how maternal age influences the efficiency of a commercial OPU-ICSI program and whether the age effect is similar to that observed for other ART in the horse. To answer this question, reproductive records of 289 mares bred b...
Durket E, Kersh K, Dembek K, Riedesel E, Silverstone A, Kraus KH.To determine the influence of plate fixation (locking or dynamic compression) and the site of application (ventral [V] or ventrolateral [VL]) on the resistance to bending of transverse mandibular fractures. Methods: Ex vivo, simple randomized study. Methods: Mandibles harvested from adult equine cadavers (n = 18). Methods: Bilateral osteotomies were created 1 cm caudal to the mental foramen and perpendicular to the long axis of each mandible. Mandibles were fixed with 1 of 3 methods: (1) VL dynamic compression plate (DCP), (2) V-DCP, or (3) VL locking compression plating (LCP). Construct...
Kuhnke S, Bär K, Bosch P, Rensing M, Borstel UKV.The study compared results of the traditional horse judging system (T) using subjective grades with those of a novel system of linear scoring (LS) using an application ("Breed Show App"). The horse's quality in relation to the total breeding aim was evaluated based on weighting factors for 57 individual traits commonly regarded in T, thus allowing immediate ranking of the horses. Results were stated as total grade in percent for both systems. One thousand nine hundred nine American Quarter horses were judged at regular breed shows with either T (n = 883), LS (n = 1,026), or both systems (n ...
Gunst S, Dittmann MT, Arpagaus S, Roepstorff C, Latif SN, Klaassen B, Pauli CA, Bauer CM, Weishaupt MA.Asymmetric forces exerted on the horse's back during riding are assumed to have a negative effect on rider-horse interaction, athletic performance, and health of the horse. Visualized on a saddle pressure mat, they are initially blamed on a nonfitting saddle. The contribution of horse and rider to an asymmetric loading pattern, however, is not well understood. The aim of this study was to investigate the effects of horse and rider asymmetries during stance and in sitting trot on the force distribution on the horse's back using a saddle pressure mat and motion capture analysis simultaneously. D...
Gunn AJ.This study involves the case of a young Thoroughbred filly who exhibited symptoms like reluctance to stand and vulva hemorrhagic discharge. The physical examination revealed normal physiological parameters, but an […]
Huang W, Yaraghi NA, Yang W, Velazquez-Olivera A, Li Z, Ritchie RO, Kisailus D, Stover SM, McKittrick J.The equine hoof has been considered as an efficient energy absorption layer that protects the skeletal elements from impact when galloping. In the present study, the hierarchical structure of a fresh equine hoof wall and the energy absorption mechanisms are investigated. Tubules are found embedded in the intertubular matrix forming the hoof wall at the microscale. Both tubules and intertubular areas consist of keratin cells, in which keratin crystalline intermediate filaments (IFs) and amorphous keratin fill the cytoskeletons. Cell sizes, shapes and IF fractions are different between tubular a...
Johnson AL, Sweeney RW, McAdams SC, Whitlock RH.Clostridium botulinum type B is estimated to cause more than 85% of cases of equine botulism in the United States, as well as many outbreaks in cattle. In this study, a quantitative real-time polymerase chain reaction for detection of the neurotoxin gene of C. botulinum type B was compared to the mouse bioassay using 45 positive and 43 negative samples of equine, bovine or associated environmental origin. The sensitivity of the qPCR assay was 96%, whereas the sensitivity of the mouse bioassay was 84%. The specificity of the qPCR assay was 95% and the specificity of the mouse bioassay was 100%....
de Leon PM, Campos VF, Dellagostin OA, Deschamps JC, Seixas FK, Collares T.In this study, polymerase chain reaction (PCR) reamplification of the first PCR product (2nd-PCR) and a qPCR assay were used to detect the sex determining region Y (SRY) gene from circulating cell-free fetal DNA (ccffDNA) in blood plasma of pregnant mares to determine fetal sex. The ccffDNA was isolated from plasma of 20 Thoroughbred mares (5-13 y old) in the final 3 mo of pregnancy (fetal sex was verified after foaling). For controls, plasma from two non-pregnant mares and two virgin mares were used, in addition to the non-template control. The 182 bp nucleotide sequence corresponding to the ...
Aleman M, Watson JL, Williams DC, LeCouteur RA, Nieto JE, Shelton GD.Fifteen horses with pituitary pars intermedia dysfunction were studied. The horses were of various breeds and between 15 and 28 years of age. Control horses matched for breed and age were studied for comparison. Evaluations included complete blood cell count and serum biochemical analysis, electromyography, and gluteus medius muscle biopsies for histochemical, morphometric, and ultrastructural analysis. No differences were found between groups of horses on routine laboratory analysis or electromyography. We demonstrated that muscle wasting in diseased horses was the result of atrophy of types ...
Han H, Chen N, Jordana J, Li C, Sun T, Xia X, Zhao X, Ji C, Shen S, Yu J, Ainhoa F, Chen H, Lei C, Dang R.Numerous studies have been conducted to investigate genetic diversity, origins and domestication of donkey using autosomal microsatellites and the mitochondrial genome, whereas the male-specific region of the Y chromosome of modern donkeys is largely uncharacterized. In the current study, 14 published equine Y chromosome-specific microsatellites (Y-STR) were investigated in 395 male donkey samples from China, Egypt, Spain and Peru using fluorescent labeled microsatellite markers. The results showed that seven Y-STRs-EcaYP9, EcaYM2, EcaYE2, EcaYE3, EcaYNO1, EcaYNO2 and EcaYNO4-were male specif...
Kosolofski HR, Gow SP, Robinson KA.A retrospective study determined the prevalence of obesity and over-conditioning in horses in Saskatoon, Saskatchewan. Body condition score (BSC) was assessed for 290 horses from the Field Service practice at the Western College of Veterinary Medicine. The median BSC of horses was 6; however, 59 (20.3%) horses were classified as over-conditioned, and 24 (8.3%) as obese. Une étude rétrospective a déterminé la prévalence de l’obésité et du surconditionnement des chevaux à Saskatoon, en Saskatchewan. La note d’état corporel (NEC) a été évaluée pour 290 chevaux à la pratique sur...
Schambony A, Gentzel M, Wolfes H, Raida M, Neumann U, Töpfer-Petersen E.Although originally described in the male rodent genital tract, cysteine-rich secretory proteins (CRISPs) are expressed in a variety of mammalian tissue and cell types. The proteins of the male genital tract have been observed associated to spermatozoa and are believed to play a role in mammalian fertilization. Here we describe the identification and primary structure of the first equine member of the CRISP family. Equine CRISP-3 is transcribed and expressed in the stallion salivary gland, in the ampulla and the seminal vesicle. It displays all 16 conserved cysteine residues and shows 82% homo...
Sosnicki AA, Lutz GJ, Rome LC, Goble DO.Until now, there has been no reliable method for histochemical determination of fiber types of single skinned muscle fibers. The major problem arises from the fact that most histochemical techniques use cross-sections of a large group of fibers and compare a given fiber with those surrounding it. This is not possible with a single skinned fiber which has been separated from a bundle to be used for mechanical analysis. A further problem is that the skinning procedure itself may alter the staining pattern. We have developed a procedure by which multiple cross-sections of single skinned fibers ca...
Fraipont A, Van Erck E, Ramery E, Fortier G, Lekeux P, Art T.A field test and a standardized treadmill test were used to assess fitness in endurance horses. These tests discriminated horses of different race levels: horses participating in races of 120 km and more showed higher values of VLA4 (velocity at which blood lactate reached 4 mmol/L) and V200 (velocity at which heart rates reached 200 beats per min) than horses of lower race levels. Un test sur le terrain et un test sur tapis roulant ont été utilisés pour évaluer la condition physique des chevaux d’endurance. Ces tests ont séparé les chevaux de différents niveaux de course : les cheva...
Collins MN, Friend TH, Jousan FD, Chen SC.Three groups of slaughter-type horses, totaling 30 mares and 29 geldings, were used to determine density effects on displacement (distance moved during a stop), falls, injuries, and orientation using a single-deck, open-topped commercial semi-trailer. Each horse was assigned to one of two treatments: high density (1.28 m(2)/horse with 14 horses) or low density (2.23 m(2)/horse with eight horses). Both treatments occurred sequentially on the same day (treatment order was alternated each trial), using the middle 2.44x7.32 m compartment of a large semi-tractor trailer. The horses were transported...
Köhne M, Kuhl J, Ille N, Erber R, Aurich C.For prevention of early conceptus loss in the horse, treatment with progestins has become common practice. In cattle, treatment with human chorionic gonadotrophin (hCG) during the early postovulatory phase stimulates endogenous progesterone synthesis, which is an important factor for maintenance of early pregnancy via stimulation of endometrial function and conceptus development. In the present study we have therefore investigated the influence of treatment with hCG either for induction of ovulation or during the early luteal phase on plasma progestin concentrations, size of the corpus luteum ...
Trommershausen Bowling A, Clark RS.Gene frequencies at 20 blood group and protein polymorphism loci (A, C, D, K, P, Q, U, Al, Tf, Pi, Xk, Es, Gc, PGD, CA, Cat, PGM, AP, Hb and PHI) are given for seven horse breeds in the United States (Thoroughbred, Arabian, Standardbred, Morgan, Quarter Horse, Paso Fino and Peruvian Paso). These data are used to calculate that the battery of tests is at least 96% effective for recognizing incorrect paternity in these breeds. In addition to paternity testing, these tests can be applied to studies of breed relationships.
Principato M.Listed and described herein are the main macroscopic lesions produced along the whole digestive tract of free-ranging horses by larvae of the five Gasterophilus spp., occurring in Umbria, a region of central Italy: Gasterophilus intestinalis, Gasterophilus nasalis, Gasterophilus pecorum, Gasterophilus inermis, Gasterophilus haemorrhoidalis. Lesions are classified on the basis of their sizes and shapes and the host's anatomic sites infested, and they are examined in relation to the developmental stages of larvae causing them. The examination of the lesions shows that it is very difficult to dif...
Qi PF, Gao XY, Ji JK, Zhang Y, Yang SH, Cheng KH, Cui N, Zhu ML, Hu T, Dong X, Yan B, Wang CF, Yang HJ, Shi WF, Zhang W.Equine coronavirus (ECoV) was first identified in the USA and has been previously described in several countries. In order to test the presence of ECoV in China, we collected 51 small intestinal samples from donkey foals with diarrhoea from a donkey farm in Shandong Province, China between August 2020 and April 2021. Two samples tested positive for ECoV and full-length genome sequences were successfully obtained using next-generation sequencing, one of which was further confirmed by Sanger sequencing. The two strains shared 100% sequence identity at the scale of whole genome. Bioinformatics an...
Pikul J, Wójtowski J, Danków R, Kuczyńska B, Lojek J.The effect of the stage of lactation, the number of foals and age of the mare on changes in the fat content and fatty acid composition of colostrum and milk of primitive Konik horses was investigated. Colostrum and milk samples from 12 lactating mares were collected at the beginning of lactation, on the days 1 and 2 after foaling and then, starting from the first month of lactation, at 4-week intervals up to the sixth month of lactation. Significant differences were observed in fat content as well as the composition of some analysed fatty acids between colostrum and milk of mares of the Konik ...
Lee HG, Choi JY, Park JW, Park TS, Song KD, Shin D, Cho BW.To examine the regulatory effects of exercise on myokine expression in horse skeletal muscle cells, we compared the expression of several myokine genes (interleukin 6 [IL-6], IL-8, chemokine [C-X-C motif] ligand 2 [CXCL2], and chemokine [C-C motif] ligand 4 [CCL4]) after a single bout of exercise in horses. Furthermore, to establish in vitro systems for the validation of exercise effects, we cultured horse skeletal muscle cells and confirmed the expression of these genes after treatment with hydrogen peroxide. Methods: The mRNA expression of IL-6, IL-8, CXCL2, and CCL4 after exercise in skelet...
Rikihisa Y, Reed SM, Sams RA, Gordon JC, Pretzman CI.In August 1986, an extensive serosurvey for prevalence of IgG and IgM antibodies against Ehrlichia risticii, the causative agent of equine monocytic ehrlichiosis (EME), was performed at 2 Ohio racetracks, River Downs (RD) and Beulah Park (BP). Of 840 horses at RD and 574 at BP, 13 and 20%, respectively, were IgG antibody-positive (by indirect fluorescent antibody test results), with antibody titer ranging from 1:20 to 1:10,240. The titer observed at highest frequency at both racetracks was 1:80. A higher proportion of horses was ill at RD (operating during the summer months) than at BP (winter...
Steiner CC, Mitelberg A, Tursi R, Ryder OA.Short divergence times and processes such as incomplete lineage sorting and species hybridization are known to hinder the inference of species-level phylogenies due to the lack of sufficient informative genetic variation or the presence of shared but incongruent polymorphism among taxa. Extant equids (horses, zebras, and asses) are an example of a recently evolved group of mammals with an unresolved phylogeny, despite a large number of molecular studies. Previous surveys have proposed trees with rather poorly supported nodes, and the bias caused by genetic introgression or ancestral polymorphi...
Smith RK, Goodship AE.Horses as equine athletes must be conditioned and trained to optimize the whole animal for the particular type of athletic activity. An extremely high proportion of all injuries in the equine athlete in general, and in the racehorse in particular, are associated with the skeletal system, many of which are believed to arise from a mismatch between the prevailing exercise levels and adaptation. To maximize performance, yet minimize injury, it is important to understand and apply the principles of functional adaptation of the component tissues of the skeletal system (bone, cartilage, tendons, and...
Merritt JS, Davies HM, Burvill C, Pandy MG.The equine distal forelimb is a common location of injuries related to mechanical overload. In this study, a two-dimensional model of the musculoskeletal system of the region was developed and applied to kinematic and kinetic data from walking and trotting horses. The forces in major tendons and joint reaction forces were calculated. The components of the joint reaction forces caused by wrapping of tendons around sesamoid bones were found to be of similar magnitude to the reaction forces between the long bones at each joint. This finding highlighted the importance of taking into account muscle...
Clark KE, Squires EL, McKinnon AO, Seidel GE.Equine embryos were recovered nonsurgically 6.5 d after ovulation (Exp. 1) and those greater than 200 microns were stored in one of three media: 1) Ham's F10 + 10% fetal calf serum (FCS) under 5% CO2, 5% O2 and 90% N2 at 24 C (Ham's F10); 2) Minimal Essential Medium with Hank's balanced salts + 10% FCS in air (MEM) at 24 C or 3) MEM at 5 C n = 10/treatment). Embryos less than or equal to 200 micron (n = 10) were bisected microsurgically; one-half of each embryo was stored in Ham's F10 and the other half in either Dulbecco's phosphate-buffered saline + 10% FCS in air at 24 C (DPBS), or MEM in a...
Stefaniuk M, Ropka-Molik K.RNA sequencing (RNA-seq) by next-generation technology is a powerful tool which creates new possibilities in whole-transcriptome analysis. In recent years, with the use of the RNA-seq method, several studies expanded transcriptional gene profiles to understand interactions between genotype and phenotype, supremely contributing to the field of equine biology. To date, in horses, massive parallel sequencing of cDNA has been successfully used to identify and quantify mRNA levels in several normal tissues, as well as to annotate genes. Moreover, the RNA-seq method has been applied to identify the ...
Peng TL, Armiladiana MM, Ruhil HH, Maizan M, Choong SS.The occurrence of Setaria digitata in a horse is reported for the first time in Malaysia. An 8-year-old Thoroughbred cross mare was referred to the University Veterinary Clinic with the primary complaint of corneal opacity and excessive eye discharge. After initial treatment with Terramycin eye ointment, corneal opacity cleared partially to reveal a moving thread-like cylindrical worm in the anterior chamber of the eye. The parasite was successfully removed surgically, and examination under the light microscope revealed that the isolated worm (length = 45 mm) was a 5th stage larva of S. ...
Fujiki Y, Rathnam P, Saxena BB.A tentative amino acid sequence of the beta-subunit of equine follicle-stimulating hormone (FSH) was derived from the sequences of tryptic, thermolytic as well as peptic, subtilisin, and chymotryptic peptides. Equine FSH-beta is analogous to human FSH-beta except six amino acid substitutions at positions 12, 16, 21, 62, 108, and 114. The amino acid sequence suggests that the hormone-specific beta-subunits of FSH are similar at the interspecies level, whereas the amino acid sequences of the hormone nonspecific alpha-subunits show variations.