Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Sanchez LC, Giguère S, Javsicas LH, Bier J, Walrond CJ, Womble AY.Gastric tonometry is commonly used in humans as an assessment of intestinal mucosal perfusion. Values in healthy foals are currently unknown. Objective: Age, enteral feeding, and omeprazole administration would significantly alter gastric tonometry measurements in neonatal foals. Methods: Nine clinically normal foals were used to assess the effect of age and feeding, and 8 similar foals were used to assess the effect of omeprazole. Methods: At 1, 7, and 14 days of age, gastric intramucosal PCO2 (PgCO2) and arterial blood gas samples were obtained at baseline, immediately after feeding milk, an...
Vidament M, Vincent P, Martin FX, Magistrini M, Blesbois E.A suitable method for the cryopreservation of donkey semen would be very valuable for the ex situ management of genetic diversity in this species. This report uses a variety of observation and trials to evaluate the effect of cryoprotectants in per-cycle pregnancy rates (PC) in equids females (jennies (donkey) and mares (horse)). This was explored by (1) comparing the results of insemination of jennies and mares with cooled or frozen donkey semen, (2) examining the possible toxic effect of the cryoprotectant (CPA) glycerol in these two species and (3) studying alternative solutions. Donkey and...
Budik S, Walter I, Tschulenk W, Helmreich M, Deichsel K, Pittner F, Aurich C.Expansion of the equine conceptus can be divided into blastocoel and yolk sac phases. The endodermal layer transforming the blastocoel into the yolk sac is completed around day 8 of pregnancy. From that time, the size of the spherical conceptus increases tremendously due mainly to the accumulation of fluid rather than cell multiplication. In this study, we have investigated the abundance and localisation of Na(+)/K(+)-ATPases and aquaporins (AQP) in the equine conceptus on days 8, 10, 12, 14 and 16 by multiplex reverse transcriptase PCR, Western blot and immunohistochemistry. During conceptus ...
Zhang YW, Davis EG, Blecha F, Wilkerson MJ.Innate immunity relies on a series of germline-encoded pattern recognition receptors (PRRs), such as Toll-like receptors (TLRs), to detect conserved microbial components. TLR9 is typically expressed intracellularly in immune cells such as dendritic cells and recognizes unmethylated bacterial or viral cytosine-phosphate-guanine DNA (CpG-DNA). To investigate innate immune responses through TLR9 signaling pathway in horses, we cloned and characterized equine TLR9. Protein sequence analysis shows that equine TLR9 has a typically conserved cytosolic Toll/interleukin-1 receptor (TIR) domain, three l...
Carluccio A, Panzani S, Tosi U, Riccaboni P, Contri A, Veronesi MC.This study was designed to establish the morphological features of the placenta of the Martina Franca jenny. Ten placentas were harvested at the time of foal delivery and examined both for gross and histological characteristics. The following factors were determined: the total weight and volume of the placenta and its components, the surface area of the allantochorion, umbilical cord length and site of insertion, and the diameter of the umbilical cord vessels and vascular pattern type. The weight of the placenta was similar to previously reported for ponies, and represented 12% of foal birth w...
Deichsel K, Palm F, Koblischke P, Budik S, Aurich C.Lipid peroxidation contributes to the damage of the sperm plasma membrane. In different species, dietary supplementation with antioxidants has been shown to improve semen quality. Therefore, we tested effects of dietary supplementation with antioxidants and l-carnitin on semen quality in Shetland pony stallions (n=6). Semen was collected twice a week over a time period of 16 weeks. From weeks 5 to 12, a special diet for stallions containing a variety of antioxidants (STALLION, Pavo Pferdenahrung GmbH, Goch, Germany; tocopherol 300 mg/day; ascorbic acid 300 mg/day; l-carnitin 4000 mg/day; folic...
Rambags BP, van Rossem AW, Blok EE, de Graaf-Roelfsema E, Kindahl H, van der Kolk JH, Stout TA.Insulin is a pancreatic hormone that classically regulates carbohydrate and fat metabolism, but also appears to play a role in various reproductive processes. A preliminary study suggested insulin production by day 10 to 18 equine conceptuses. The aim of the present study was to examine the hypothesis that insulin is the conceptus signal responsible for maternal recognition of pregnancy (MRP) in the mare, or otherwise influences reproductive cyclicity during the MRP period. Six Warmblood mares were treated daily during days 7 to 17 after ovulation of two successive oestrous cycles with either ...
Giacomoni EH, Fernández-Stolz GP, Freitas TR.The genetic variability for a sample of 227 animals from three populations of Pantaneiro horses was estimated using data from 10 microsatellite loci. The number of alleles and the proportion of heterozygosity indicated high variability. A total of 91 alleles were found, with a significantly high mean number of alleles. The mean polymorphic information content was 0.7 and the paternity exclusion probability was 99.3%. The inbreeding coefficient (F(IS)) was low for the three populations: Ipiranga (F(IS) = 0.147), Nova Esperança (F(IS) = 0.094) and Promissão (F(IS) = 0.108). Genetic differentia...
Parker M, Redhead ES, Goodwin D, McBride SD.Horses displaying an oral stereotypy were tested on an instrumental choice paradigm to examine differences in learning from non-stereotypic counterparts. Stereotypic horses are known to have dysfunction of the dorsomedial striatum, and lesion studies have shown that this region may mediate response-outcome learning. The paradigm was specifically applied in order to examine learning that requires maintenance of response-outcome judgements. The non-stereotypic horses learned, over three sessions, to choose a more immediate reinforcer, whereas the stereotypic horses failed to do so. This suggests...
Auty H, Mundy A, Fyumagwa RD, Picozzi K, Welburn S, Hoare R.Horses kept for recreational riding purposes by a wildlife tourism company in a heavily tsetse fly-infested region of north-western Tanzania were systematically monitored to investigate the occurrence, presentation and management of tsetse-transmitted trypanosomosis. During a 23-month period, 18 clinical cases were diagnosed (Trypanosoma brucei or Trypanosoma congolense were identified) and treated and trypanosomes were implicated of involvement in four deaths. Pyrexia consistently aided early detection (17 cases). Ataxia, weight loss and anaemia were seen in chronic cases and conferred a poor...
Dugarjaviin M, Yang H.Mongolian horse is a kind of important breed resource of local horses in our country. It has a lot of advantages such as powerful endurance, rough feeding resistance, and strong disease resistance. These advantages have become driving force for in-depth study on Mongolian horse. Genetic diversity can reflect all the genetic information of a species or a variety, namely, it reflects the richness of genetic diversity and confirms the degree of uniqueness of genetic resources through genetic markers. This paper introduces the progress in the study on genetic diversity of Mongolian horse in many a...
Stafford KJ, Erceg VH.The veterinary program at the Institute of Veterinary, Animal and Biomedical Sciences, Massey University, New Zealand, is 4.5 years long. Animal handling and restraint are taught to first-year veterinary students via lectures and practical sessions. Students are taught to catch, restrain, and hold the five important domesticated species (horses, cattle, sheep, dogs, and cats) in a series of five practical sessions, each lasting about three hours. Safety is emphasized. Students are taught in groups of 10-12, and generally the tutors demonstrate the various techniques of restraint and then super...
Chapman HM, Taylor EG, Buddle JR, Murphy DJ.The ability to handle animals safely, competently, and with confidence is an essential skill for veterinarians. Poor animal-handling skills are likely to compromise credibility, occupational health and safety, and animal welfare. In the five-year veterinary science degree at Murdoch University, animal handling is taught in a prerequisite unit in the second semester of the second year. From 2008, however, this unit will be taught in the first year of the five-year course. Students are taught to handle sheep, cattle, pigs, and horses safely and competently. Each student receives 30 hours of form...
Haussler KK, Behre TH, Hill AE.'Soring' is the term used to describe the application of an irritant to the distal forelimbs of gaited horses with the sole intent of inflicting pain and inducing altered gait, illegally practiced in Tennessee Walking Horses. Objective methods for the detection of limb pain due to this practice are, however, lacking. Objective: To assess whether Tennessee Walking Horses respond to manual pressures<or=10 kg/cm2 and to establish reference mechanical nociceptive thresholds (MNTs) within the pastern region. Methods: In 25 mature Tennessee Walking Horses in which no irritant had been applied, MN...
Kummer M, Gygax D, Lischer C, Auer J.Hoof preparation and shoe fit are integral to the long term soundness and athletic ability of horses. The farrier influences the balance of the hoof by both the trimming and the shoeing procedure. The goal of this study was to investigate and quantify the influence of the farrier on hoof parameters by trimming. Forty Warmblood horses ranging in age from 6-12 years and withers height 162-172cm were included. They were divided into six groups consisting of six or seven horses each, shod by one of six different farriers. The hoofs of the horses were radiographed twice using a standardised method ...
Andrews FM, Buchanan BR, Elliott SB, Al Jassim RA, McGowan CM, Saxton AM.Volatile fatty acids, byproducts of carbohydrate fermentation by resident bacteria, have been implicated in causing nonglandular (NG) gastric ulcers. Lactic acid (LA), also produced by stomach bacteria, may cause gastric ulcers when exposed to the equine NG mucosa. Objective: To investigate the in vitro effects of LA on equine NG mucosa bioelectric properties, sodium transport and tissue resistance. Methods: Gastric tissues obtained from 13 mature horses were studied in Ussing chambers. Short-circuit current (Isc) and potential difference (PD) were measured, and electrical resistance (R) and c...
Licka T, Frey A, Peham C.The pattern of electromyographic activity of the equine long back muscle at the walk has not yet been reported. The aim of this study was to use surface electromyography to measure activity of the longissimus dorsi muscles of horses walking on a treadmill. Fifteen horses without back pain were used and electromyographs were recorded bilaterally from the longissimus dorsi muscles at the level of T12, T16 and L3. Mean electromyograph activity and mean motion were calculated for each horse. At the walk, only one maximum activity for each longissimus dorsi muscle was detected during each motion cy...
Boyde A, Firth EC.The aim was to survey articular calcified cartilage (ACC) and subchondral bone in the palmar and dorsal regions of the condyles of the third metacarpal bone (Mc3) of young horses with minimal or no signs of musculo-skeletal abnormality. Back-scattered electron scanning electron microscopy (BSE SEM) was conducted on polymethyl methacrylate-embedded mediolateral slices and macerated wedges of the right distal Mc3 from seven each of trained and untrained 2-year-old Thoroughbred horses. Furrows or grooves visible to the naked eye in the mineralizing front (MF) of ACC are the commonest "lesion" and...
Lu Z, Branscum AJ, Shuck KM, Zhang J, Dubovi EJ, Timoney PJ, Balasuriya UB.Two previously developed TaqMan fluorogenic probe-based 1-tube real-time reverse transcription polymerase chain reaction (real-time RT-PCR) assays (T1 and T2) were compared and validated for the detection of Equine arteritis virus (EAV) nucleic acid in equine semen and tissue culture fluid (TCF). The specificity and sensitivity of these 2 molecular-based assays were compared to traditional virus isolation (VI) in cell culture. The T1 real-time RT-PCR had a higher sensitivity (93.4%) than the T2 real-time RT-PCR (42.6%) for detection of EAV RNA in semen. However, the T1 real-time RT-PCR was les...
O'Grady SE.Proper farriery promotes a healthy functional foot and biomechanical efficiency and prevents lameness. Because the equine veterinarian is responsible for the soundness of the horse, a working knowledge of farriery becomes essential. A thorough knowledge of traditional horseshoeing enables the veterinarian to interact with the farrier at the farrier's level; this ultimately enhances and promotes quality hoof care. This article focuses on fundamental farriery and recognizing subtle changes in hoof conformation that can be used to preserve the integrity of the hoof capsule, along with the structu...
Richardson DW.To repair equine fractures successfully, surgeons traditionally have done aggressive open approaches with maximal internal fixation to achieve adequate stability and comfort. Although the need for stability is unquestionable, newer technologies and imaging modalities have allowed improvement in the biology of internal fixation in selected fractures and arthrodeses.
Smith RK, Goodship AE.Horses as equine athletes must be conditioned and trained to optimize the whole animal for the particular type of athletic activity. An extremely high proportion of all injuries in the equine athlete in general, and in the racehorse in particular, are associated with the skeletal system, many of which are believed to arise from a mismatch between the prevailing exercise levels and adaptation. To maximize performance, yet minimize injury, it is important to understand and apply the principles of functional adaptation of the component tissues of the skeletal system (bone, cartilage, tendons, and...
Fortier LA, Smith RK.After tendon injury, the scar tissue that replaces the damaged tendon results in a substantial risk for reinjury. The goal of regenerative therapies is to restore normal structural architecture and biomechanical function to an injured tissue. Successful restoration processes for any tissue are thought to recapitulate those of development, in which there are spatial and temporal interactions between scaffold, growth factors, and cell populations.
Scott M.Quarter horses used for western performance competitions commonly sustain a variety of musculoskeletal injuries. It is important for the veterinarian to have an understanding of some of the breed characteristics and the nature of the competitions in which individual horses are being used so as to diagnose, treat, and prevent injury as effectively as possible.
Weishaupt MA.The skill to diagnose lameness in horses is paramount for every equine practitioner. Early recognition of locomotor deficiencies plays a central role in sports medicine management, preventing deterioration of the disease or catastrophic injuries. Horses use characteristic compensatory movements of specific body parts to decrease loading of the affected limb. This article describes the underlying changes in intra- and interlimb coordination and the resulting load redistribution between the limbs. This enables the practitioner to better understand the changes in movement associated with lameness...
Mellish MA, Adreani CM.A 5-year-old, Quarter horse mare was treated for severe cellulitis secondary to a gunshot wound near the right humerus. The bullet was not retrieved due to the risk of damaging the radial nerve or elbow joint. Despite the presence of the bullet, the mare resumed athletic soundness once the infection had resolved. Traitement d’une blessure par balle chez une jument. Une jument Quarter horse âgée de 5 ans a été traitée pour une cellulite sévère à la suite d’une blessure par balle près de l’humérus droit. Le projectile n’a pas été retiré par crainte de léser le nerf radial o...
Varhac R, Antalík M.A relation between pH-induced conformational transitions of horse heart ferricytochrome c and the kinetics of external ligand coordination to heme iron was investigated by optical spectroscopy, circular dichroism and viscometry. The dependencies of both the association, k (a), and dissociation rate constants of cyanide binding on pH were determined from kinetic measurements. The association rate constant exhibits a bell-shaped form of dependence on pH in the region where this protein unfolds. The maximum of the dependence of k (a) on pH is found to be coincident with the pK values of conformat...
We compared the composition and content of fatty acids (FAs) in the liver, muscles, and subcutaneous fat of Yakut horses inhabiting extreme environment in the Cryolithozone. Essential linoleic and alpha-linolenic acids, supplied to horses with their food, were accumulated in different tissues. Linoleic acid was accumulated in the liver but alpha-linolenic acid was accumulated in muscle and subcutaneous fat. Such a distribution indicates different roles of these fatty acids in the metabolism of horses. Yakut horse meat is a valuable dietary product owing to its fatty acid composition and conten...
Takasawa T, Fukushi K, Shiokawa H.A crystalline creatine kinase was obtained from equine skeletal muscle. The enzyme was homogeneous, as judged by ultracentrifugation and disc electrophoresis on polyacrylamide gel. The crystalline enzyme had a specific activity of 110 units per mg of protein, that is, 14-fold purification over the crude extract of equine skeletal muscle. The molecular weight of the enzyme was determined to be 84,600 by the conventional low-speed sedimentation equilibrium method, and s020,w was 5.32S. Eight cysteine residues were found on amino acid analysis, two of which were essential for the enzymatic activi...
Anderson MG.The distribution of lactic dehydrogenase, aldolase and creatine kinase in various horse tissues was determined. Using polyacrylamide gel electrophoresis the lactic dehydrogenase and creatine kinase isoenzyme composition of horse serum, taken before and after exercise, was studied. Horse tissue isoenzyme patterns were also obtained. By comparing tissue and serum patterns, skeletal muscle was found to be the tissue of origin of the increase in serum lactic dehydrogenase and creatine kinase observed after exercise.
Kruger K, Stegmann GF.A partial intravenous protocol was used successfully to maintain anaesthesia in 5 healthy horses. Horses were premedicated with acepromazine, romifidine and butorphanol, induced with guaifenesin and ketamine and maintained on a constant rate infusion of lidocaine, ketamine and medetomidine together with halothane inhalation anaesthesia. Mean end-tidal halothane concentration to maintain a surgical plane of anaesthesia was 0.8 +/- 0.2%. Mean dobutamine requirement to maintain mean arterial pressure above 9.31 kPa was 0.42 +/- 0.3 microg/kg/min. The administration of relatively low doses of lido...
Laing JA, Leech FB.In a group of 852 Thoroughbred mares, 69-7% had foals which survived beyond 6 weeks, 7-1% had dead, premature or full-term foals and 1-1% had foals which died within 6 weeks after birth. Possible factors which may have influenced these data are mentioned.
Rej R, Rudofsky U, Magro A, Prendergast J.Aminotransferase activities were measured in the serum of two- to three-year-old Thoroughbred fillies and colts during a four week period of peak training for flat racing. Aspartate aminotransferase (AspAT, EC 2.6.1.1), mitochondrial aspartate aminotransferase (m-AspAT) and alanine aminotransferase (AlaAT, EC 2.6.1.2) activities in serum were measured and the relative proportions of apoenzyme and holoenzyme were determined. The aminotransferase activities were increased only slightly immediately following exercise. This small and immediate post exercise increase in activity did not vary greatl...
Irwin DH, Howell DW.Several indications for swimming horses are recalled. A satisfactory pool and the technique for its use are described. Some observations on the effect on swimming are offered.
Uhlhorn H, Eksell P, Carlsten J.A longitudinal scintigraphic and radiographic study of distal radial physeal activity in 14 Standardbred trotters was conducted to quantify and visually evaluate physeal uptake around the time of physeal closure. Initial intense 99mTc-HDP uptake was seen, followed by a rapid decrease of activity starting at a mean age of 20 months. Mean physeal to distal diaphyseal ROI and line profile ratios compared to ratios of mature horses decreased from 2.8 to 1.4 at the time of radiographic closure at 24-32 months. Ratios then gradually decreased until values of mature horses were reached at the end of ...
Wnuk M, Villagómez DA, Bugno-Poniewierska M, Tumidajewicz P, Carter TF, Slota E.Nucleolar organizing regions (NORs) containing rDNA gene clusters have been assigned to the equine autosomes ECA1, ECA28, and ECA31. Active NORs (Ag-NORs) are associated with argyrophilic proteins, which allow them to be readily identified using silver staining techniques. Fluorescence in situ hybridization (FISH) for rDNA can also be used to visualize all NOR clusters in the nucleus, regardless of whether they are active or inactive. The present study analyzed the distribution and behavior of equine Ag-NOR and NOR clusters in horse spermatozoa and during male meiosis by FISH and silver staini...
J Lacy K, Parlane NA, Riley CB, Gee EK, Roberts JM, McIlwraith CW.CellTrace Violet™ is a commonly used fluorescent dye used with flow cytometry to identify cell proliferation. Activated equine lymphocytes were examined using flow cytometry, microscopy and tritiated thymidine proliferation assays. CellTrace Violet™ was incorporated into the equine lymphocytes effectively. Equine lymphocytes proliferated when activated with pokeweed mitogen, but did not proliferate when previously stained with CellTrace Violet™. Serial dilutions of CellTrace Violet™ did not eliminate the inhibition of activated lymphocytes. Equine lymphocyte viability was greater than ...
Monteiro RA, Cunha RM, Guerra MMP, de Almeida VM, Peña-Alfaro CE, Silva SV.The aim of this study was to test equine semen cryopreservation techniques for the conservation of donkey germplasm. Ejaculates of three male donkeys were used (n = 18; six ejaculates per donkey; six repetitions), collected by the artificial vagina method. To remove the seminal plasma, the ejaculates were split and submitted to filtration or centrifugation methods. To assess the freezing method, each fraction was submitted to the automated system or the conventional system, and groups were formed: automated centrifuge, automated filtrate, conventional centrifuge and conventional filtrate. Af...
McKenzie EC.Polyuria and polydipsia provide a diagnostic challenge for the equine clinician. This article describes the various known causes of polyuria and polydipsia in horses and provides a description of a systematic diagnostic approach for assessing horses with polyuria and polydipsia to delineate the underlying cause. Treatment and management strategies for addressing polyuria and polydipsia in horses are also described.
Morton DB.Animals with relatively highly developed brains are likely to experience some degree of self-awareness and the ability to think. As well as being interesting in its own right, self-consciousness matters from an ethical point of view, since it can give rise to forms of suffering above and beyond the immediate physical sensations of pain or distress. This article surveys the evidence for animal self-consciousness and its implications for animal welfare.
Hodgson D, Howe S, Jeffcott L, Reid S, Mellor D, Higgins A.Oral administration of altrenogest for oestrus suppression in competition horses is believed to be widespread in some equestrian disciplines, and can be administered continuously for several months during a competition season. To examine whether altrenogest has any anabolic or other potential performance enhancing properties that may give a horse an unfair advantage, we examined the effect of oral altrenogest (0.044 mg/kg), given daily for a period of eight weeks, on social hierarchy, activity budget, body-mass and body condition score of 12 sedentary mares. It was concluded that prolonged ora...
Smith BL, Jones JH, Carlson GP, Pascoe JR.To determine whether body direction in a trailer affects the degree to which a horse is excited (and presumably stressed) during transport, heart rates were measured in 8 Thoroughbred geldings transported over a 32-km route of county roads while tethered facing forward or backward in a 4-horse stock trailer. Heart rates also were measured on the horses while they were tethered facing forward or backward in the same trailer while it was parked. Heart rates decreased during the first 10 minutes for both groups, and remained stable after the first 15 minutes. Heart rates were not significantly di...
Winsco KN, Coverdale JA, Wickersham TA, Shelton JL.Twenty-four Quarter Horse weanlings (120 ± 10 d) were blocked by age into 4 groups (n=6) for a 56-d trial to evaluate the influence of dietary Met concentration on growth and N balance. Weanling horses were housed by block and individually fed concentrates twice daily at 1.75% BW (as-fed basis). Weanling horses were randomly assigned to 1 of 4 concentrate treatments: basal (0.20 Met), basal + 0.03% Met (0.23 Met), basal + 0.07% Met (0.27 Met), and basal + 0.11% Met (0.31 Met). Diets were formulated to be isonitrogenous and isocaloric and contained equal amounts of Lys and Thr. Coastal bermuda...
Gutiérrez Rincón JA, Vives Turcó J, Muro Martínez I, Casas Vaqué I.The three main Olympic horse riding disciplines are dressage, jumping, and three-day eventing (including dressage, cross country and jumping). In the jumping discipline (obstacle race), the 'team' (horse rider) is judged under the different conditions that might take place in a varied run. The horse is expected to show power and ability; the rider must show riding skill and good physical condition. However, the different conditions encountered by the rider during competition (duration of event, continuous isometric working level, especially in the inferior trunk, lead us to consider the need f...
Frontiers in geneticsJanuary 29, 2021
Volume 12 619947 doi: 10.3389/fgene.2021.619947
Dugué M, Dumont Saint Priest B, Crichan H, Danvy S, Ricard A.Functional longevity is essential for the well-being of horses and the satisfaction of riders. Conventional selection using longevity breeding values calculated from competition results is not efficient because it takes too long to obtain reliable information. Therefore, the objective was to identify early criteria for selection. We assessed two types of early criteria: gait traits of young horses and QTLs. Thus, our aim was to estimate the genetic correlation between gait traits and longevity and to perform a genome-wide association study (GWAS) for longevity. Measurements of gaits by acceler...