Analyze Diet

Topic:Horses

"Horses" is a broad topic that encompasses various aspects of equine biology, behavior, and management. This category includes studies on the anatomy, physiology, and genetics of horses, as well as their behavior, nutrition, and care. Research in this area may also cover the historical and cultural significance of horses, their roles in agriculture, sport, and therapy, and the challenges associated with their conservation and welfare. The page aggregates peer-reviewed research articles and scholarly studies that explore the multifaceted relationships between humans and horses, examining both scientific and socio-economic perspectives.
Hydration and electrolyte balance in horses during an endurance season.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 98-104 doi: 10.1111/j.2042-3306.2010.00198.x
Robert C, Goachet AG, Fraipont A, Votion DM, Van Erck E, Leclerc JL.Limited information exists about the physiological training-induced changes in electrolyte balance of horses competing in long distance endurance races. Objective: To determine the effects of endurance training and racing on hydration and electrolyte balance in horses. Methods: Blood and urine were sampled at rest in 8 endurance horses before training and after two 11 week training periods (T1 and T2). Each training was followed by a 120 km endurance ride and horses were sampled before, during and 2 h after the rides. Blood was analysed for packed cell volume (PCV), total protein (TP), urea, c...
Electromyography activity of the equine splenius muscle and neck kinematics during walk and trot on the treadmill.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 455-461 doi: 10.1111/j.2042-3306.2010.00263.x
Zsoldos RR, Kotschwar AB, Kotschwar A, Groesel M, Licka T, Peham C.Skeletal muscle activity can be concentric or eccentric, anisometric or isometric and correlation of the equine splenius muscle activity with the movement of its effector joints at walk and trot has not yet been fully characterised. Objective: Investigating activity of the splenius muscle together with kinematics of head and cranial neck at walk and trot. Methods: Kinematics and surface electromyography were measured in 6 horses (8-20-years-old, 450-700 kg) without signs of neck pain. Markers were placed on left and right crista facialis, and on left and right cervical vertebrae 1 and 3. Head ...
Heel movement in horses: comparison between glued and nailed horse shoes at different speeds.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 431-435 doi: 10.1111/j.2042-3306.2010.00243.x
Yoshihara E, Takahashi T, Otsuka N, Isayama T, Tomiyama T, Hiraga A, Wada S.It has been suggested that the heel of the horse's hoof expands in the stance phase and this reduces the concussion at impact and helps pump blood into the hoof. Therefore, farriers usually leave a gap in the heel region when using the traditional nailed shoe. Recently glued shoes which are attached firmly to the heel have been developed and these could restrict heel movement. Objective: To compare the degree of mediolateral heel movement between glued and nailed shoes. Methods: Seven Thoroughbreds were used. Either their fore- or hind hooves were shod with plain aluminium shoes, attached firs...
Effects of collecting blood into plastic heparinised vacutainer tubes and storage conditions on blood gas analysis values in horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 91-97 doi: 10.1111/j.2042-3306.2010.00248.x
Noël PG, Couëtil L, Constable PD.Plastic heparinised vacutainer tubes are used for blood gas analysis in horses. This collection method may not be ideal because influx of atmospheric O(2) through the permeable plastic wall of the vacutainer tube and loss of CO(2) into the gas phase above the blood sample should increase blood PO(2) and decrease PCO(2), respectively. Objective: To determine the effects of collecting blood into plastic vacutainer tubes and storage conditions on blood gas analysis values. Methods: Blood was obtained from 6 healthy horses and tonometered at 37 °C with 12% O(2) and 5% CO(2). Three ml aliquots of ...
Effects of fence height on total and free iodothyronine changes in horses after experimental show jumping sessions.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 110-115 doi: 10.1111/j.2042-3306.2010.00252.x
Ferlazzo A, Medica P, Cravana C, Fazio E.Involvement of thyroid function on performance warrants further investigation as limited data exists on the effects of showjumping on the dynamics of total and free iodothyronines. Objective: To investigate the response of circulating total and free iodothyronines in horses to experimental showjumping sessions and compare with the effects normally induced by competition and determine if fence height has any effect. Methods: Using a randomised crossover study design 6 trained horses were studied during experimental showjumping sessions over 10 fences of different height: 1.00 m (Session 1), 1.1...
The role of tendon stiffness in development of equine locomotion with age.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 556-560 doi: 10.1111/j.2042-3306.2010.00296.x
Addis PR, Lawson SE.The flexor tendons support the metacarpophalangeal (MCP) and distal interphalangeal (DIP) joints during stance phase and since tendon stiffness and strain changes with age, it is likely that kinematics are also age-dependent. Objective: Maximum MCP and DIP angles decrease in the young horse, plateau in the mature horse and increase towards senescence. Methods: The distal limbs of 57 walking horses age 3-212 months were filmed and digitised with an automated tracking system. Maximum MCP and DIP angles during stance phase were used to calculate strain in the superficial and deep digital flexor t...
Mechanical properties of the equine superficial digital flexor tendon relate to specific collagen cross-link levels.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 538-543 doi: 10.1111/j.2042-3306.2010.00175.x
Thorpe CT, Stark RJ, Goodship AE, Birch HL.Damage to the flexor tendons, particularly the superficial digital flexor tendon (SDFT), is one of the most common musculoskeletal injuries sustained by horses competing in all disciplines. Our previous work has shown that SDFTs from different individuals show a wide variation in mechanical strengths; this is important clinically as it may relate to predisposition to injury. The high mechanical strength of tendon relies on the correct orientation of collagen molecules within fibrils and stabilisation by the formation of chemical cross-links between collagen molecules. It is not known whether t...
Influence of girth strap placement and panel flocking material on the saddle pressure pattern during riding of horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 502-509 doi: 10.1111/j.2042-3306.2010.00173.x
Byström A, Stalfelt A, Egenvall A, Von Peinen K, Morgan K, Roepstorff L.Saddle fit is well recognised as an important factor for the health and performance of riding horses. However, only few studies have addressed general effects of different saddle construction details within a group of horses. Objective: To assess the influence of girth strap placement, traditional vs. v-system, and panel flocking material, wool vs. synthetic foam, on the saddle pressure pattern during riding. Methods: Six horses were ridden by 3 riders in sitting and rising trot and sitting canter. Saddle pressure was measured with 3 different saddle variants: 1) wool flocked panels and tradit...
Plasma aldosterone concentration and cardiovascular response to low sodium intake in horses in training.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 329-334 doi: 10.1111/j.2042-3306.2010.00244.x
Jansson A, Johannisson A, Kvart C.Horses in training lose large amounts of sodium but little is known about the cardiovascular response to low sodium intake. Objective: To investigate the effect of low sodium intake on plasma aldosterone (pAldo) concentrations and the cardiovascular system of athletic horses, and to identify markers of low sodium intake. Methods: Seven Standardbred geldings in training (trained twice a week) were randomly offered a standardised diet supplemented (NaS, 58 mg Na/kg bwt) and not supplemented (NaN, 3 mg Na/kg bwt) with NaCl for 5 weeks in a changeover design. Blood samples were taken once a week a...
Sweat composition in Arabian horses performing endurance exercise on forage-based, low Na rations.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 382-386 doi: 10.1111/j.2042-3306.2010.00208.x
Spooner HS, Nielsen BD, Schott HC, Harris PA.Excessive sweat loss during endurance exercise may lead to electrolyte disturbances and previous research suggests dietary factors may affect hydration status. While investigating the effect of dietary fibre type on hydration status, sweat samples were collected which allowed for the evaluation of sweat composition in horses consuming forage-based, low sodium (Na) rations. Objective: To investigate sweat composition in Arabian horses performing endurance type exercise while fed forage-based, rations low in Na. Methods: Six 2-year-old Arabian horses were fed, according to a replicated 3 × 3 La...
Observer agreement for detection of cardiac arrhythmias on telemetric ECG recordings obtained at rest, during and after exercise in 10 Warmblood horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 208-215 doi: 10.1111/j.2042-3306.2010.00172.x
Trachsel DS, Bitschnau C, Waldern N, Weishaupt MA, Schwarzwald CC.Frequent supraventricular or ventricular arrhythmias during and after exercise are considered pathological in horses. Prevalence of arrhythmias seen in apparently healthy horses is still a matter of debate and may depend on breed, athletic condition and exercise intensity. Objective: To determine intra- and interobserver agreement for detection of arrhythmias at rest, during and after exercise using a telemetric electrocardiography device. Methods: The electrocardiogram (ECG) recordings of 10 healthy Warmblood horses (5 of which had an intracardiac catheter in place) undergoing a standardised ...
Metacarpal geometry changes during Thoroughbred race training are compatible with sagittal-plane cantilever bending.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 407-411 doi: 10.1111/j.2042-3306.2010.00209.x
Merritt JS, Davies HM.Bending of the equine metacarpal bones during locomotion is poorly understood. Cantilever bending, in particular, may influence the loading of the metacarpal bones and surrounding structures in unique ways. Objective: We hypothesised that increased amounts of sagittal-plane cantilever bending may govern changes to the shape of the metacarpal bones of Thoroughbred racehorses during training. We hypothesised that this type of bending would require a linear change to occur in the combined second moment of area of the bones for sagittal-plane bending (I) during race training. Methods: Six Thorough...
Cardiac arrhythmias in clinically healthy showjumping horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 196-201 doi: 10.1111/j.2042-3306.2010.00185.x
Buhl R, Meldgaard C, Barbesgaard L.Electrocardiographic reference values of clinically normal horses during exercise are a prerequisite when evaluating horses with poor performance or horses presented with cardiac abnormalities (e.g. valvular regurgitation). No published studies have reported cardiac rhythms of clinically normal showjumping horses. Objective: To demonstrate the feasibility of obtaining exercise electrocardiogram (ECG) in normal horses during showjumping and also to document the prevalence and frequency of arrhythmias in association with exercise. Methods: Thirty-four healthy showjumping horses aged 4-13 years (...
Comparison of net anaerobic energy utilisation estimated by plasma lactate accumulation rate and accumulated oxygen deficit in Thoroughbred horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 62-69 doi: 10.1111/j.2042-3306.2010.00261.x
Ohmura H, Mukai K, Takahashi T, Matsui A, Hiraga A, Jones JH.Accumulated O(2) deficit (AOD) and plasma lactate accumulation rate (PLAR) are alternative methods for estimating net anaerobic energy utilisation (NAEU) in exercising horses. How they compare or their accuracy is unknown. Objective: We hypothesised net anaerobic energy utilisation calculated by PLAR (NAUE(PLAR)) is equivalent to NAUE estimated by AOD (NAUE(AOD)). Methods: Six Thoroughbred horses ran at identical supramaximal speeds (118% aerobic capacity) until exhaustion for 2 runs while breathing normoxic (NO, 21% O(2)) or hyperoxic (HO, 26% O(2)) gas. Jugular blood was sampled at 15 s inte...
Efficacy of single-dose intravenous phenylbutazone and flunixin meglumine before, during and after exercise in an experimental reversible model of foot lameness in horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 601-605 doi: 10.1111/j.2042-3306.2010.00232.x
Foreman JH, Grubb TL, Inoue OJ, Banner SE, Ball KT.Objective blinded efficacy data during exercise are lacking on the use of single-dose i.v. nonsteroidal anti-inflammatory drugs (NSAIDs) before, during and after exercise. Objective: Single i.v. doses of either phenylbutazone (PBZ) or flunixin meglumine (FM) would prove more efficacious than negative saline control (SAL) before, during and after exercise in a reversible model of foot lameness. Methods: Six Quarter Horse mares had lameness induced by tightening a set screw against a heart bar shoe 1 h prior to treatment. Randomised blinded treatments included PBZ (4.4 mg/kg bwt i.v.), FM (1.1 m...
Experimental mild pulmonary inflammation promotes the development of exercise-induced pulmonary haemorrhage.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 235-239 doi: 10.1111/j.2042-3306.2010.00295.x
McKane SA, Slocombe RF.Histological studies of exercise-induced pulmonary haemorrhage (EIPH) reveals inflammatory infiltrates within areas of lung that contain haemorrhage. This has resulted in the hypothesis that pulmonary inflammation could cause EIPH or contribute to an increased risk or severity of EIPH. Objective: To determine whether experimentally-induced pulmonary inflammation predisposes the lung to haemorrhage during exercise, by evaluating the bronchoalveolar lavage (BAL) cytology of normal and inflamed regions of lung following high speed treadmill exercise. Methods: Transendoscopic airway inoculations o...
Physiological measurements and prevalence of lower airway diseases in Trotters with dorsal displacement of the soft palate.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 246-255 doi: 10.1111/j.2042-3306.2010.00276.x
Courouce-Malblanc A, Deniau V, Rossignol F, Corde R, Leleu C, Maillard K, Pitel PH, Pronost S, Fortier G.Dorsal displacement of the soft palate (DDSP) is one of the most common obstructive conditions of the upper respiratory tract in the racehorse. This condition has a complex aetiology which may be caused or exacerbated by pharyngeal inflammation. Additionally, lower respiratory airway diseases may be associated with DDSP thereby contributing to exercise intolerance in these horses. Objective: The aim of this study was to measure physiological variables during a standardised exercise test and to assess the prevalence and consequences of lower respiratory airway disease in horses with DDSP. Metho...
Specific immuno-extraction followed by enzymatic detection (SIEFED) of myeloperoxidase and mitochondrial complex I in muscular microbiopsies: preliminary results in endurance horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 296-302 doi: 10.1111/j.2042-3306.2010.00270.x
Franck T, Votion DM, Ceusters J, De La Rebière de Pouyade G, Mouithys-Mickalad A, Niesten A, Fraipont A, VAN Erck E, Goachet AG, Robert C, Serteyn D.Intense exercise in horses induces an increase of creatine kinase (CK) and stimulation of neutrophils which release the strong oxidant enzyme, myeloperoxidase (MPO) into the blood. It is not known whether active MPO is found in equine muscles and whether oxidant activity of neutrophils could affect muscular tissues and mitochondrial activity. Objective: Specific immuno-extraction followed by enzymatic detection (SIEFED) methods will be employed for the first time to assess both the normal range of MPO and mitochondrial complex I (MCI) activities in equine muscular microbiopsies and to study th...
Age related decreases in thermoregulation and cardiovascular function in horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 220-227 doi: 10.1111/j.2042-3306.2010.00259.x
McKeever KH, Eaton TL, Geiser S, Kearns CF, Lehnhard RA.Older horses have an increased risk of hyperthermia due to impaired cardiovascular function. While many studies have investigated thermoregulation in horses during exercise, none have investigated the effects of ageing. Objective: To test the hypothesis that there is a difference in thermoregulation during exercise and plasma volume (PV) in young and old horses. Methods: Study 1: 6 young (Y, 7.7 ± 0.5 years) and 5 old (O, 26.0 ± 0.8 years) unfit Standardbred mares (507 ± 11 kg, mean ± s.e.) ran on a treadmill (6% grade, velocity calculated to generate a work rate of 1625 watts) until core ...
Effects of crude protein intake from forage-only diets on muscle amino acids and glycogen levels in horses in training.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 341-346 doi: 10.1111/j.2042-3306.2010.00283.x
Essén-Gustavsson B, Connysson M, Jansson A.There is little information about the influence of crude protein (CP) intake on glycogen and free pool amino acid concentrations in the muscle of horses in training. High energy forage-only diets may be an alternative to concentrate rich diets and may provide high levels of CP. Objective: To study the effect of feeding 2 forage-only diets, containing either high or moderate CP concentrations on glycogen and free pool amino acid concentrations in the muscle. Methods: Two high energy forage-only diets based on high-energy grass forage were fed for 23 days in a crossover design to 6 Standardbred ...
Differences in the metabolic properties of gluteus medius and superficial digital flexor muscles and the effect of water treadmill training in the horse.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 665-670 doi: 10.1111/j.2042-3306.2010.00229.x
Borgia LA, Valberg SJ, Essen-Gustavsson B.Flexor tendon injury may be due to flexor muscle fatigue, contributing to fetlock joint hyperextension and tendon damage. A water treadmill provides resistance training on flexor tendon muscles, which might reduce the risk of tendon injury. Objective: To determine the effect of water treadmill training on the properties of the gluteal and superficial digital flexor (SDF) muscles and on cardiocirculatory response to a standardised exercise test. Methods: Five healthy unfit horses were trained on a water treadmill for 5 days/week for 4 weeks, starting with 5 min/day increasing to 20 min/day. Bef...
Effect of a 120 km endurance race on plasma and muscular neutrophil elastase and myeloperoxidase concentrations in horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 275-279 doi: 10.1111/j.2042-3306.2010.00269.x
Serteyn D, Sandersen C, Lejeune JP, de la Rebière de Pouyade G, Ceusters J, Mouithys-Mickalad A, Niesten A, Fraipont A, van Erck E, Goachet AG....Intense physical exercise can induce the degranulation of neutrophils leading to an increase in plasma concentration of the neutrophil marker enzymes myeloperoxidase (MPO) and elastase (ELT). These enzymes have pro-oxidative and pro-inflammatory properties and may play a role in the exercised-induced muscular damage. Objective: To measure MPO and ELT concentrations in plasma and muscles of endurance horses and to correlate them to the extent of exercise-induced muscular damage. Methods: Seven endurance horses qualified on 120 km races were tested in this study. Neutrophil count, serum creatine...
Effect of feed restriction on plasma dantrolene concentrations in horses.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 613-617 doi: 10.1111/j.2042-3306.2010.00262.x
McKenzie EC, Garrett RL, Payton ME, Riehl JH, Firshman AM, Valberg SJ.Dantrolene sodium is used to prevent exertional rhabdomyolysis in predisposed horses. Food intake might negatively impact dantrolene bioavailability in horses; however, prolonged feed restriction might be detrimental to performance. Objective: To determine a minimum duration of feed restriction that would optimise plasma dantrolene concentrations in horses after nasogastric administration. It was hypothesised that feed restriction for 4, 8 or 12 h before dantrolene administration would result in higher plasma dantrolene concentrations than achieved with no feed restriction before treatment. Me...
Efficacy of spinal manipulation and mobilisation on trunk flexibility and stiffness in horses: a randomised clinical trial.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 695-702 doi: 10.1111/j.2042-3306.2010.00241.x
Haussler KK, Martin CE, Hill AE.Spinal mobilisation and spinal manipulative therapy (SMT) are being applied to horses; however, there are limited objective measures of their effects on spinal mobility or stiffness in actively ridden horses. Objective: To quantify passive spinal movements induced during dorsoventral mobilisation of the trunk and to identify any potential effects of SMT on measures of spinal mobility within the thoracolumbar region in standing horses. We hypothesise that displacement amplitudes will be significantly increased across vertebral levels after SMT, compared to spinal mobilisation only within the co...
Effects of prior exercise on insulin-mediated and noninsulin-mediated glucose uptake in horses during a hyperglycaemic clamp.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 129-134 doi: 10.1111/j.2042-3306.2010.00218.x
Geor RJ, Stewart-Hunt L, McCutcheon LJ.There is limited information about factors regulating glucose utilisation post exercise in horses. Objective: To determine the effects of a single bout of moderate intensity exercise on measures of insulin-mediated (IMGU) and noninsulin-mediated (NIMGU) glucose uptake during a hyperglycaemic clamp (HC). Methods: Hyperglycaemic clamps were administered in random order to 8 Standardbreds under 4 conditions: 1) rest, insulinopenia (R-L); 2) rest, hyperinsulinaemia (R-H); 3) post exercise (45 min at ∼ 50% VO2peak), insulinopenia (Ex-L) and 4) post exercise, hyperinsulinaemia (Ex-H). In the R-L a...
Endurance veterinarians detect physiologically compromised horses in a 160 km ride.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 6-11 doi: 10.1111/j.2042-3306.2010.00225.x
Barnes A, Kingston J, Beetson S, Kuiper C.This study investigated the physiology of endurance horses competing in warm weather over technical terrain, a situation where horses may become metabolically compromised. Objective: There will be changes in physiological, haematological and biochemical variables as horses progress through the 160 km ride and horses detected clinically at the veterinary inspections as metabolically compromised will have significant differences in measured laboratory variables compared to horses that complete the ride successfully. Methods: Forty-eight horses competing in the Australian Tom Quilty 160 km endura...
Comparison of kinematic symmetry index calculations and the effects of straight and circular trotting.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 482-487 doi: 10.1111/j.2042-3306.2010.00195.x
Walker AM, Wilson AM, Pfau T.When assessing lameness in horses, left to right ratios of kinematic parameters are often used to quantify movement symmetry. Different methods of symmetry related measures have been proposed and inertial sensor data was used to evaluate the application of 3 methods of symmetry calculation during straight and circular trotting. Objective: To compare 3 sensor based methods of symmetry index calculation to assess; tuber coxae vs. sacrum motion, the effects of circular trotting and effect of using whole trials in place of individual stride calculations. Methods: Inertial sensors were attached to ...
The effect of water height on stride frequency, stride length and heart rate during water treadmill exercise.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 662-664 doi: 10.1111/j.2042-3306.2010.00194.x
Scott R, Nankervis K, Stringer C, Westcott K, Marlin D.Water treadmill exercise is often incorporated into rehabilitation programmes for horses yet little is known about the biomechanical and physiological responses to water walking. Objective: To establish whether stride frequency (SF) reached steady state as a result of 6 introductory water treadmill sessions and then to investigate the effect of increasing water height on SF, stride length (SL) and heart rate (HR). Methods: Nine horses with no previous experience of water treadmills completed 6 sessions of walking for between 15 and 30 min. Each horse was fitted with a leg mounted accelerometer...
Nutrient intake during an elite level three-day event competition is correlated to inflammatory markers and antioxidant status.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 116-122 doi: 10.1111/j.2042-3306.2010.00193.x
Williams CA, Burk AO.Dietary intake and feeding management practices could affect the degree of physiological stress and subsequent performance of high level 3-day event horses. Objective: To assess whether a relationship exists between dietary intake levels of selected nutrients and the inflammatory and antioxidant status in horses competing in a high level 3-day event. Methods: Riders competing in a CCI2*/CCI3* 3-day event (n = 35) answered a nutritional management survey conducted by the investigators at the competition. Actual and recommended intakes of vitamin E, potassium (K), calcium (Ca), phosphorus (P) an...
Effects of short-term training on thermoregulatory and sweat responses during exercise in hot conditions.
Equine veterinary journal. Supplement    May 27, 2011   Issue 38 135-141 doi: 10.1111/j.2042-3306.2010.00235.x
McCutcheon LJ, Geor RJ.There is evidence that extensive training in cool conditions results in improvements to heat dissipation that contribute to successful acclimatisation. In horses, the effects of a less extensive training regimen have not been determined. Objective: This study investigated whether 10 consecutive days of moderate intensity treadmill training in cool conditions would improve thermoregulatory and sweating responses of horses to exercise in the heat. Methods: Six unfit Thoroughbred horses completed a standardised treadmill exercise test (SET) in hot, dry conditions (32-34 °C, 45-55% RH) before (SE...