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Topic:Imaging Techniques

Imaging techniques in horses encompass a range of diagnostic tools used to visualize the internal structures and systems of equine patients. These techniques assist in the diagnosis, monitoring, and treatment planning of various conditions affecting horses. Common imaging modalities include radiography (X-rays), ultrasonography, magnetic resonance imaging (MRI), and computed tomography (CT). Each technique offers unique advantages in terms of resolution, depth of penetration, and the type of tissues that can be examined. For instance, radiography is frequently used for assessing bone structures, while ultrasonography is often employed for soft tissue evaluation. MRI and CT provide detailed cross-sectional images, aiding in the assessment of complex anatomical regions. This page compiles peer-reviewed research studies and scholarly articles that explore the application, advancements, and comparative efficacy of imaging techniques in equine veterinary medicine.
Population of follicles and luteal structures during the oestrous cycle of mares detected by three-dimensional internal structure microscopy.
Anatomia, histologia, embryologia    May 28, 2009   Volume 38, Issue 3 214-218 doi: 10.1111/j.1439-0264.2008.00924.x
Hirano Y, Kimura J, Nambo Y, Yokota H, Nakamura S, Takemoto S, Himeno R, Mishima T, Matsui M, Miyake YI.The structure of the equine ovary is different from that of other mammals in its extremely large size, the presence of ovarian fossa and the inverted location of its cortex and medulla. A three-dimensional internal structure microscopy (3D-ISM), which consists of a computer-controlled slicer, a CCD camera, a laser disc recorder and a PC, is very useful for the observation of the internal structures in equine ovaries. In addition, the three-dimensional images of follicles and corpus luteum (CL) reconstructed by the segmentation technique can clarify the spatial arrangement in the equine ovary. ...
Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device.
Journal of veterinary science    May 23, 2009   Volume 10, Issue 2 161-163 doi: 10.4142/jvs.2009.10.2.161
Kimura J, Kakusho N, Yamazawa K, Hirano Y, Nambo Y, Yokota H, Himeno R.The 3D internal structure microscopy (3D-ISM) was applied to the equine ovary, which possesses peculiar structural characteristics. Stereolithography was applied to make a life-sized model by means of data obtained from 3D-ISM. Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary. Photopolymerized resin models of equine ovaries produced by stereolithography can clearly show the internal structure and spatial localizations in the ovary. The understanding of the spatial relationship between the ovulation fossa and follicles and/or corpora lutea i...
Intra- and interobserver agreement in the interpretation of navicular bones on radiographs and computed tomography scans.
Equine veterinary journal    May 8, 2009   Volume 41, Issue 2 124-129 doi: 10.2746/042516408x345125
Groth AM, May SA, Weaver MP, Weller R.Criteria for the radiographic evaluation of navicular bones in horses have been published to standardise classification of radiographic signs. However, intra- and interobserver agreement have not been established. Objective: To determine intra- and interobserver agreement in the evaluation of radiographic and computed tomographic (CT) navicular changes. It was hypothesised that: 1) intraobserver agreement would be better than interobserver agreement; 2) agreement would be better for CT than for radiography; and 3) pathological changes would be recognised with greater certainty with CT. Methods...
Characterization of the magic angle effect in the equine deep digital flexor tendon using a low-field magnetic resonance system. Spriet M, McKnight A.Three isolated equine limbs were imaged with a low-field magnetic resonance system with a vertical magnetic field. Each limb was scanned in multiple positions with mild variation of the angle between the magnetic field and the long axis of the limb. When the long axis of the limb was not perpendicular to the magnetic field, a linear hyperintense signal was present at the palmar aspect of one of the deep digital flexor tendon lobes, at the level of the navicular bone and collateral sesamoidean ligaments, in proton density and T1-weighted pulse sequences. With increased angulation of the limb, t...
Magnetic resonance imaging of the initial active stage of equine laminitis at 4.7 T. Arble JB, Mattoon JS, Drost WT, Weisbrode SE, Wassenaar PA, Pan X, Hunt RJ, Belknap JK.Equine laminitis is a severely debilitating disease. There is a poor understanding of the underlying pathophysiology, and traditional imaging modalities have limited diagnostic capacity. High field strength magnetic resonance (MR) imaging allows direct visualization of the laminae, which other modalities do not. This would prove useful both in assessment of clinical patients and in further investigation into the pathophysiology of the disease. The objective of this study was to characterize the anatomic changes within the equine foot associated with the initial active stage of laminitis. Image...
Diagnostic accuracy of digital photography and image analysis for the measurement of foot conformation in the horse.
Equine veterinary journal    January 24, 2009   Volume 40, Issue 7 623-628 doi: 10.2746/042516408x313625
White JM, Mellor DJ, Duz M, Lischer CJ, Voute LC.Rigorous evaluation of practicable methods for the objective assessment of foot conformation has not been performed. Objective: To assess the practicability, precision and accuracy of the process of obtaining measurements of horses' feet using photography and image processing software. Methods: Precision study: Lateral photographs of horses' feet were obtained twice by 2 veterinary surgeons (image acquisition - IAc). Photographs were analysed by 2 masked veterinary surgeons on 2 occasions (image analysis - IAn). Measurements were compared within and between operators for self and non-self acqu...
Monitoring of the repair process of surgically created lesions in equine superficial digital flexor tendons by use of computerized ultrasonography.
American journal of veterinary research    January 6, 2009   Volume 70, Issue 1 37-48 doi: 10.2460/ajvr.70.1.37
van Schie HT, Bakker EM, Cherdchutham W, Jonker AM, van de Lest CH, van Weeren PR.To evaluate quantitative ultrasonography for objective monitoring of the healing process and prognostication of repair quality in equine superficial digital flexor (SDF) tendons. Methods: 6 horses with standardized surgical lesions in SDF tendons of both forelimbs. Methods: Healing was monitored for 20 weeks after surgery by use of computerized ultrasonography. Pixels were categorized as C (intact fasciculi), B (incomplete fasciculi), E (accumulations of cells and fibrils), or N (homogenous fluid or cells). Four scars with the best quality of repair (repair group) and 4 scars with the lowest q...
Virtual histology by means of high-resolution X-ray CT.
Journal of microscopy    December 20, 2008   Volume 232, Issue 3 476-485 doi: 10.1111/j.1365-2818.2008.02142.x
Cnudde V, Masschaele B, De Cock HE, Olstad K, Vlaminck L, Vlassenbroeck J, Dierick M, Witte YD, Van Hoorebeke L, Jacobs P.Micro-CT is a non-destructive technique for 3D tomographic investigation of an object. A 3D representation of the internal structure is calculated based on a series of X-ray radiographs taken from different angles. The spatial resolution of current laboratory-used micro-CT systems has come down over the last years from a few tens of microns to a few microns. This opens the possibility to perform histological investigations in 3D on a virtual representation of a sample, referred to as virtual 3D histology. The advantage of micro-CT based virtual histology is the immediate and automated 3D visua...
Fluorangiographic study of the ocular fundus in normal horses.
Veterinary ophthalmology    December 17, 2008   Volume 11 Suppl 1 2-7 doi: 10.1111/j.1463-5224.2008.00621.x
Molleda JM, Cervantes I, Galán A, Tardón R, Gallardo JM, Martín-Suárez EM.To describe the protocol and appearance of fluorescein angiography (FA) in normal horses. Methods: A total of 25 healthy horses aged between 5 and 15 years. Methods: The horses were sedated with 15 microg/kg detomidine and 50 microg/kg butorphanol and dilated with topical tropicamide 1%. All angiograms were recorded after intravenous bolus injection of 10 mg/kg of fluorescein sodium solution. Results: Two successive angiographic phases could be discerned: the choriopapillary phase, starting at 46.95 +/- 9.48 s, and the retinal vascular phase, starting at 47.79 +/- 10.38 s. The retinal vascular...
Imaging diagnosis–avulsion of the origin of the popliteal tendon in a horse. Ebling AJ, Slack J, Reef VB.No abstract available
Is a magic angle effect observed in the collateral ligaments of the distal interphalangeal joint or the oblique sesamoidean ligaments during standing magnetic resonance imaging? Smith MA, Dyson SJ, Murray RC.Collagen fibers oriented at 55 degrees to the static magnetic field (B0) are characterized by an artifactual increase in signal intensity due to the magic angle effect. We hypothesized that there would be increased signal intensity in the collateral ligaments of the distal interphalangeal joint and oblique sesamoidean ligaments when these ligaments were at angles approaching 55 degrees to a horizontal B0 during standing magnetic resonance (MR) imaging. MR imaging was performed on four cadaver forelimbs in a 0.27 T standing system. Transverse and dorsal images were obtained using various sequen...
Magnetic resonance imaging of distal sesamoidean ligament injury. Smith S, Dyson SJ, Murray RC.Distal sesamoidean ligament injury is a recognized cause of lameness but diagnosis using ultrasonography is sometimes difficult. Herein, we describe the normal appearance of the distal sesamoidean ligaments on magnetic resonance (MR) images and the changes that occur when the ligaments are injured. The appearance of the distal sesamoidean ligaments on MR images from 66 control horses and 58 horses with distal sesamoidean desmitis were described and the cross-sectional area and signal intensity of the ligaments measured. In control horses, the ligaments had a characteristic appearance and stron...
Computed tomographic anatomy of the temporomandibular joint in the young horse.
Equine veterinary journal    November 26, 2008   Volume 40, Issue 6 566-571 doi: 10.2746/042516408x322166
Rodríguez MJ, Latorre R, López-Albors O, Soler M, Aguirre C, Vázquez JM, Querol M, Agut A.The equine temporomandibular joint (TMJ) and its surrounding structures can be difficult to investigate in cases with a clinical problem related to the region. Little previous attention has been given either to a computed tomographic (CT) imaging protocol for the joint or an interpretation of the structures displayed in CT images of the normal joint. Objective: To provide a CT atlas of the normal cross-sectional anatomy of the equine TMJ using frozen and plastinated sections as anatomical reference. Methods: Eight TMJs from 4 immature pure-bred Spanish horses were examined by helical CT. Scans...
ISSLS prize winner: positron emission tomography and magnetic resonance imaging for monitoring interbody fusion with equine bone protein extract, recombinant human bone morphogenetic protein-2, and autograft.
Spine    November 13, 2008   Volume 33, Issue 25 2683-2690 doi: 10.1097/BRS.0b013e31817fce91
Foldager C, Bendtsen M, Zou X, Zou L, Olsen AK, Munk OL, Stødkilde-Jørgensen H, Bünger C.Prospective and randomized experimental study with anterior lumbar interbody fusion in a porcine model. Objective: To assess the early time-course of spinal fusion with equine bone protein extract (COLLOSS E), recombinant human bone morphogenetic protein-2 (rhBMP-2), and autograft using quantitative methods of positron emission tomography (PET)/computed tomography and magnetic resonance imaging (MRI). Background: Different growth and differentiation factors are currently being used for inducing bone formation in spinal fusion. However, the mechanisms and time-course of bone formation using the...
Computed tomography-guided brain biopsy for in vivo diagnosis of a cholesterinic granuloma in a horse.
Journal of the American Veterinary Medical Association    September 18, 2008   Volume 233, Issue 6 950-954 doi: 10.2460/javma.233.6.950
Vanschandevijl K, Gielen I, Nollet H, Vlaminck L, Deprez P, van Bree H.An 8-year-old warmblood mare was evaluated following an acute onset of neurologic abnormalities. Results: Computed tomography of the head revealed large masses in both lateral ventricles, and a presumptive diagnosis of cholesterinic granuloma was made. Results: Freehand biopsy of the intracranial masses was performed under computed tomographic guidance, and histologic examination of biopsy specimens confirmed the diagnosis. No adverse effects associated with the brain biopsy procedure were encountered. Clinical signs resolved, and long-term follow-up did not reveal any recurrence of neurologic...
Novel optical imaging technique to determine the 3-D orientation of collagen fibers in cartilage: variable-incidence angle polarization-sensitive optical coherence tomography.
Osteoarthritis and cartilage    July 14, 2008   Volume 17, Issue 1 33-42 doi: 10.1016/j.joca.2008.05.005
Ugryumova N, Jacobs J, Bonesi M, Matcher SJ.To investigate a novel optical method to determine the three dimensional (3-D) structure of articular cartilage collagen non-destructively. Methods: Polarization-sensitive optical coherence tomography was used to determine the apparent optical birefringence of articular cartilage for a number of different illumination directions. A quantitative method based on the theory of light propagation in uniaxial crystalline materials was validated on equine flexor tendon. Qualitative maps of fiber polar and azimuthal orientation at sites on the posterior and anterior segments of the equine third metaca...
Comparison of mean bone densities of three preparations of the distal portion of the equine third metacarpal bone measured by use of quantitative computed tomography.
American journal of veterinary research    July 3, 2008   Volume 69, Issue 7 891-893 doi: 10.2460/ajvr.69.7.891
Drum MG, Les CM, Park RD, McIlwraith CW, Kawcak CE.To evaluate whether cutting equine subchondral bone to demarcate specific regions of interest (ROIs) influences the mean density for that bone as measured via quantitative computed tomography (QCT). Sample population-2 metacarpophalangeal joints from equine cadavers. Methods: The distal portion of the third metacarpal bone of each intact metacarpophalangeal joint was scanned via CT to simulate in vivo conditions. Each joint was subsequently disarticulated and dissected, and the distal portion of the dissected third metacarpal bone in air was scanned. Then, six 1-cm(2) areas representing ROIs w...
Use of magnetic resonance imaging to diagnose the cause of proximal metacarpal pain in a standardbred trotter.
The Veterinary record    June 17, 2008   Volume 162, Issue 24 790-792 doi: 10.1136/vr.162.24.790
Coudry V, Denoix JM, Didierlaurent D, Rossignol F, Audigié F.No abstract available
Ultrasonographic diagnosis–idiopathic muscular hypertrophy of the small intestine in a miniature horse. Dechant JE, Whitcomb MB, Magdesian KG.No abstract available
Ultrasonographic diagnosis–desmitis of the accessory ligament of the deep digital flexor tendon in a hindlimb in a horse. Jorgensen AJ, Diaz OS, Reef VB.No abstract available
Gastrointestinal ultrasonography in normal adult ponies. Epstein K, Short D, Parente E, Reef V, Southwood L.The objective of this study was to determine the characteristics based on ultrasonographic examination of the stomach, duodenum, jejunum, cecum, and peritoneal fluid in normal adult ponies. Abdominal ultrasonographic examination was performed in nine unsedated standing ponies. The duodenum was examined at three sites and the jejunum in 12 regions. Wall thickness, contractility, distention, and luminal contents were recorded. Stomach wall thickness and location, cecal wall thickness, and peritoneal fluid location and character were recorded. Statistical analysis was performed. Wall thicknesses ...
Novel oblique radiographic projection of the temporomandibular articulation of horses.
The Veterinary record    June 3, 2008   Volume 162, Issue 22 714-716 doi: 10.1136/vr.162.22.714
Ramzan PH, Marr CM, Meehan J, Thompson A.A novel radiographic projection is described for assessing part of the temporomandibular articulation of horses. It minimises the superimposition of osseous structures of the cranium that limit the usefulness of conventional projections and permits improved imaging in cases of suspected disease.
Effects of exercise vs experimental osteoarthritis on imaging outcomes.
Osteoarthritis and cartilage    May 27, 2008   Volume 16, Issue 12 1519-1525 doi: 10.1016/j.joca.2008.04.015
Kawcak CE, Frisbie DD, Werpy NM, Park RD, McIlwraith CW.To identify changes in imaging outcomes in a controlled model of osteoarthritis (OA) vs exercise. Methods: Sixteen 2-year-old horses were randomly assigned to an exercise control (n=8) or an exercise OA (n=8) group. All horses had middle carpal joints arthroscopically explored and an osteochondral fragment was induced in one middle carpal joint of the OA group. All horses were treadmill exercised for the duration of the study (91 days). Clinical, radiographic, nuclear scintigraphic, computed tomographic and magnetic resonance imaging (MRI) examinations were performed and outcomes of these were...
Diagnostic imaging of the equine fetlock region using radiography and ultrasonography. Part 2: the bony disorders.
Veterinary journal (London, England : 1997)    May 7, 2008   Volume 181, Issue 2 123-136 doi: 10.1016/j.tvjl.2008.01.017
Vanderperren K, Saunders JH.The metacarpophangeal/metatarsophalangeal (fetlock) joint in the horse is commonly associated with equine lameness and diagnostic imaging is routinely used to investigate disorders of the joint and its surrounding tissues. This review describes the osseous disorders of the fetlock as well as the technical aspects of taking radiographic and ultrasonographic images of the different lesions. In current clinical practice, a combination of radiography and ultrasonography is still the most frequently used approach to arrive at a diagnosis.
Evaluation of technetium Tc 99m-labeled biotin for scintigraphic detection of soft tissue inflammation in horses.
American journal of veterinary research    May 2, 2008   Volume 69, Issue 5 639-646 doi: 10.2460/ajvr.69.5.639
Kleine LG, Solano M, Rusckowski M, Hunt KE, Johnson KL, Kirker-Head CA.To evaluate the use of technetium Tc 99m-labeled EDTA-biotin monomer ((99m)Tc-EB1) as a scintigraphic imaging agent for soft tissue inflammatory lesions in horses. Methods: 6 healthy adult horses. Methods: First (phase 1), the agent's safety and blood-tissue clearance and an appropriate imaging protocol were determined in 6 horses. Each horse was injected with (99m)Tc-EB1 (1.1 GBq, IV, once); images were acquired at intervals during the following 24-hour period. Subsequently (phase 2), inflammation was induced via injection of 200 mg (10 mL) of mepivacaine (0.4 mg/kg) into the right neck muscu...
Diagnostic imaging of the equine fetlock region using radiography and ultrasonography. Part 1: Soft tissues.
Veterinary journal (London, England : 1997)    April 28, 2008   Volume 181, Issue 2 111-122 doi: 10.1016/j.tvjl.2008.03.005
Vanderperren K, Saunders JH.The equine fetlock is the joint most commonly associated with lameness. Although the fetlock is regarded as a simple joint, diagnosis of a fetlock disorder can be a challenge and various imaging modalities are routinely used to arrive at an accurate diagnosis. This review describes the principal disorders affecting the soft tissues of the fetlock region and addresses some of the technical aspects involved in taking radiographic and ultrasonographic images of the different soft tissue lesions. A combination of radiography and ultrasonography is still the most commonly used diagnostic approach i...
Simulation of local anaesthetic nerve block of the infraorbital nerve within the pterygopalatine fossa: anatomical landmarks defined by computed tomography.
Research in veterinary science    March 26, 2008   Volume 85, Issue 3 399-406 doi: 10.1016/j.rvsc.2008.02.008
Staszyk C, Bienert A, Bäumer W, Feige K, Gasse H.To provide anaesthesia of the maxillary cheek teeth, a local block of the infraorbital nerve in the pterygopalatine fossa has been suggested. The aim of this study was to re-examine the anatomy of the pterygopalatine fossa, giving special attention to relevant arteries, veins and nerves; simulate the infiltration of an anaesthetic by injecting a contrast medium; improve the injection technique to avoid puncturing of relevant anatomical structures. Five heads and two living horses were investigated using contrast medium injections and computed tomography (CT). Needles were inserted using two in...
Measurement of equine laminar blood flow and vascular permeability by use of dynamic contrast-enhanced computed tomography.
American journal of veterinary research    March 4, 2008   Volume 69, Issue 3 371-377 doi: 10.2460/ajvr.69.3.371
Kruger EF, Puchalski SM, Pollard RE, Galuppo LD, Hornof WJ, Wisner ER.To define the reference range for laminar blood flow (BF) and vascular permeability (VPM) in horses without laminitis by use of dynamic contrast-enhanced computed tomography (CT). Methods: 9 adult horses that were not lame and had no abnormalities of the laminae or phalanges detectable via radiographic examination. Methods: Each horse was anesthetized by use of a routine protocol. Horses were placed in right or left lateral recumbency with the dependent forelimb in the CT gantry; only 1 limb of each horse was scanned. Serial 10-mm collimated transverse CT images were acquired at the same locat...
Less invasive techniques for equine fracture repair and arthrodesis.
The Veterinary clinics of North America. Equine practice    March 4, 2008   Volume 24, Issue 1 177-189 doi: 10.1016/j.cveq.2007.11.004
Richardson DW.To repair equine fractures successfully, surgeons traditionally have done aggressive open approaches with maximal internal fixation to achieve adequate stability and comfort. Although the need for stability is unquestionable, newer technologies and imaging modalities have allowed improvement in the biology of internal fixation in selected fractures and arthrodeses.
Fracture configurations of the equine radius and tibia after a simulated kick.
Veterinary and comparative orthopaedics and traumatology : V.C.O.T    February 22, 2008   Volume 21, Issue 1 49-58 doi: 10.3415/VCOT-07-03-0022
Fuerst AE, Oswald S, Jäggin S, Piskoty G, Michel S, Auer JA.The objective of this postmortem study was to determine the fracture configurations of the equine radius and tibia after a simulated kick. Fracture configurations of 35 radii and 36 tibiae from 19 adult horses were evaluated after a simulated kick in an experimental ex-vivo study. The bones were dissected, the proximal and distal ends were embedded in resin, fixed horizontally and preloaded in compression, and a steel impactor, designed to simulate a shod equine hoof, was dropped from a height of three to six metres onto the diaphysis. The experiments were filmed with a high-speed camera (30,0...
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