Topic:Temperature
Temperature regulation in horses involves physiological processes that maintain the animal's core body temperature within a narrow, optimal range despite external environmental changes. Horses, being homeothermic animals, rely on mechanisms such as sweating, respiration, and blood flow adjustments to dissipate excess heat or conserve warmth. These thermoregulatory processes are influenced by factors such as exercise, humidity, and ambient temperature. Variations in temperature can affect a horse's performance, health, and overall well-being. This page aggregates peer-reviewed research studies and scholarly articles that explore the mechanisms, effects, and implications of temperature regulation in equine physiology.
Thermoregulation in the horse in response to exercise. Conversion of stored energy into mechanical energy during exercise is relatively inefficient with approximately 80% of the energy being given off as heat. Relative to many species the horse suffers an apparent disadvantage by possessing a high metabolic capacity yet a small surface area for dissipation of heat, particularly as evaporation of sweat is the major method of heat dissipation. Under most conditions of exercise at least two-thirds of the metabolic heat load is dissipated via this means with sweat losses of more than 10 l h-1 reported. The remaining exercise-induced heat load must be ...
The transition from inhomogeneous to homogeneous kinetics in CO binding to myoglobin. Heme proteins react inhomogeneously with ligands at cryogenic temperatures and homogeneously at room temperature. We have identified and characterized a transition from inhomogeneous to homogeneous behavior at intermediate temperatures in the time dependence of CO binding to horse myoglobin. The turnover is attributed to a functionally important tertiary protein relaxation process during which the barrier increases dynamically. This is verified by a combination of theory and multipulse measurements. A likely biological significance of this effect is in the autocatalysis of the ligand release p...
Effects of storage time and temperature on ionized calcium concentration in equine blood, plasma, and serum. Stability of ionized calcium (Ca2+) concentrations and pH values in equine venous samples (n = 12 in each group) stored at 4 C for 3, 9, 24, and 48 hours (blood, plasma, and serum) or for 240 hours (plasma and serum), and at -20 C for 240 hours (plasma and serum), was studied. Storage of equine blood, plasma, and serum samples at 4 C for up to 48 hours and of serum samples at 4 C for up to 240 hours, despite appreciable pH changes, was associated with < 1.5% change in blood, plasma, and serum Ca2+ concentrations. Therefore, Ca2+ concentration in equine blood, plasma, and serum samples store...
Effect of time and storage temperature on cholinesterase activity in blood from normal and organophosphorus insecticide-treated horses. Delays between time of sampling and time of testing are common; therefore, the length of time that blood can be stored at various temperatures was evaluated for effects on cholinesterase activity. Six horses were treated with 16 g of trichlorfon per os, 6 horses were treated with 15 g of dichlorvos per os, and 10 horses were untreated controls. The cholinesterase activity in whole blood from each horse was measured using an adaptation of the Ellman colorimetric method. The blood from each horse was then divided into 3 groups and stored at 5 C (refrigerated), 20 C (room temperature), or 38 C (i...
Survey of selected design and ventilation characteristics of racehorse stables in the Pretoria, Witwatersrand, Vereeniging area of South Africa. Stables housing more than 20 horses in training were surveyed in the Pretoria, Witwatersrand, Vereeniging area of South Africa. Most racehorses were kept in loose boxes, bedded on straw or sawdust and remained indoors while the stables were cleaned. The average floor area was 13 m2 and airspace was 55 m3 per animal. The average predicted minimum air change rate by natural convection in calm winds was 7.0 air changes per hour, which was reduced to 2.2 when the doors and shutters were closed. The survey showed that many of the stables had been built without due consideration to factors that migh...
Comparative study of the oxyhaemoglobin dissociation curve of four mammals: man, dog, horse and cattle. 1. The entire oxygen dissociation curve (ODC) and the effects of temperature, pH and 2,3-diphosphoglycerate (DPG) on this curve, have been compared in four mammalians: man, dog, horse and cattle. 2. If the oxyphoric capacities are similar between these species (around 1.39 ml O2/gHb), their P50, measured in standard conditions, i.e. at pH 7.4; pCO2 40 mmHg and T 37 degrees C, varies between 23.8 (+/- 0.8) mmHg for the horse, 25.0 (+/- 1.4) mmHg for cattle, 26.6 (+/- 1.2) for man and 28.8 (+/- 2.6) mmHg for the dog. 3. The higher dispersion of the dog's P 50 is due to difference between breeds;...
A comparison of the reduction in immunoglobulin (IgG) concentration of frozen equine plasma treated by three thawing techniques. The IgG concentration of plasma from 13 mares was measured by radial immunodiffusion when fresh and after storage at -4 degrees C and thawing by 3 methods. There was no significant (P > 0.05) reduction in the IgG concentration when plasma was thawed over 6 hours at 22 degrees C (1352.9 +/- 101.6 mg/dL) (mean +/- SEM) compared with the fresh sample (1369.5 +/- 88.1 mg/dL). In contrast, there was a significant (P < 0.05) reduction in the IgG concentration of plasma that was rapidly thawed at 57 degrees C over 50 minutes (1142.9 +/- 66.2 mg/dL), or placed in a microwave oven for 20 to 25 minutes ...
Partially folded states of equine lysozyme. Structural characterization and significance for protein folding. Despite their homologous structure, c-type lysozymes and alpha-lactalbumins have been found to differ profoundly in their unfolding behavior, in that the alpha-lactalbumins readily enter a partially unfolded collapsed state (the "molten globule"), whereas lysozymes unfold cooperatively to a highly unfolded state. The calcium-binding property of lysozyme from equine milk provides an evolutionary link between the two families of proteins. We demonstrate here that equine lysozyme undergoes a two-stage unfolding transition upon heating or in the presence of guanidine hydrochloride that is highly d...
The effectiveness of gentamicin or polymyxin B for the control of bacterial growth in equine semen stored at 20 degrees C or 5 degrees C for up to forty-eight hours. Semen from three stallions was used to evaluate the effectiveness of two antibiotics added to semen extender for samples stored at 20 degrees C or 5 degrees C for up to 48 hours. Each ejaculate was divided into six different treatments: semen+extender (SE); SE+gentamicin (100 micrograms/mL); SE+polymyxin B (1000 units/mL); and each of the above treatments inoculated with Pseudomonas aeruginosa ATCC 27853. Sampling of diluted semen for bacteriological analysis was performed after 2, 8, 24 and 48 hours of preservation at either temperatures. The presence of nonspecific bacteria was noted after t...
Effects of sample collection and handling on concentration of osteocalcin in equine serum. A commercially available radioimmunoassay kit for measurement of human osteocalcin was validated for use in horses. For accurate measurement of equine serum osteocalcin, blood samples may be collected at a temperature between 20 and 25 C, then centrifuged within 90 minutes; serum may be stored at -20 C in plastic tubes for up to 26 weeks. Serum may be thawed and refrozen up to 5 times without significant change in measured equine serum osteocalcin concentration. Assay sensitivity was 0.16 ng/ml. Recovery of bovine osteocalcin standard added to equine serum was linear. Intra-assay coefficient o...
Effect of temperature, duration of storage and sampling procedure on ammonia concentration in equine blood plasma. The effect of storage duration at different storage temperatures on the plasma ammonia concentration of equine EDTA whole blood, EDTA plasma and heparin plasma samples was investigated. Further, the effect of jugular vein compression before and during blood sampling on the plasma ammonia values was evaluated. In EDTA whole blood kept at 4 degrees C there was no significant increase of ammonia content after 6 hours of storage, whereas the increase was already significant 3 hours after collection if the EDTA whole blood was kept at 20-22 degrees C. EDTA plasma samples stored at 20-22 degrees C, ...
Structural relaxation and nonexponential kinetics of CO-binding to horse myoglobin. Multiple flash photolysis experiments. The geminate recombination kinetics of CO-myoglobin strongly deviates from single exponential behavior in contrast to what is expected for unimolecular reactions (1). At low temperatures, this result was attributed to slowly exchanging conformational states which differ substantially in barrier height for ligand binding. Above 160 K the kinetics apparently slow down with temperature increase. Agmon and Hopfield (2) explain this result in terms of structural relaxation perpendicular to the reaction coordinate, which enhances the activation energy. In their model, structural relaxation homogeniz...
Effects of intra-articularly administered endotoxin on clinical signs of disease and synovial fluid tumor necrosis factor, interleukin 6, and prostaglandin E2 values in horses. In each of 4 horses, sterile synovitis was induced by intra-articular injection of 3 micrograms of Escherichia coli endotoxin (lipopolysaccharide, LPS) into one antebrachiocarpal joint; an equal volume (2 ml) of phosphate-buffered saline solution (PBSS) was injected into the opposite, control carpus. Blood and 1.5 ml of synovial fluid were obtained at postinjection hours (PIH) 0, 2, 4, 8, 12, 18, 42, 66, and 144. Synovial fluid sample collection was accomplished by use of an indwelling, intra-articular catheter through PIH 12, and by arthrocentesis subsequently. Joint fluid samples were analyz...
Dissipation of metabolic heat in the horse during exercise. Horses were exercised at 40, 65, and 90% of their maximum O2 uptake (VO2max) until moderately fatigued (approximately 38, 15, and 9 min, respectively) to assess heat loss through different routes. Approximately 4,232, 3,195, and 2,333 kcal of heat were generated in response to exercise at these intensities. Of this, approximately 7, 16, and 20% remained as stored heat 30 min postexercise. Respiratory heat loss, estimated from the temperature difference between blood in the pulmonary and carotid arteries and the cardiac output, was estimated to be 30, 19, and 23% of the heat produced during exe...
[Hemolytic properties of bacteria belonging to the Acinetobacter genus]. Direct and intermediate hemolytic activity of 526 strains of Acinetobacter was investigated. Their ability to produce lipase and lecithinase was also studied. Measurements were performed parallely on human, horse, sheep and bovine erythrocytes. Direct hemolytic activity was exhibited by 16% of tested strains (17 out of 24 strains of A. haemolyticus). Human, sheep and bovine erythrocytes were useful for testing the hemolytic activity of Acinetobacter. The hemolysis was occurring faster and was visible more frequently during incubation at 37 degrees C. Indirect hemolytic activity was observed in...
Effect of temperature on the transmission of western equine encephalomyelitis and St. Louis encephalitis viruses by Culex tarsalis (Diptera: Culicidae). The extrinsic incubation rate (inverse of the time in days from infection to median transmission) of western equine encephalomyelitis (WEE) and St. Louis encephalitis (SLE) viruses by laboratory strains of Culex tarsalis Coquillett increased as a linear function of incubation temperatures from 10 to 30 degrees C. The estimated temperatures for zero transmission thresholds (intercept of the X axis) were 10.9 and 14.9 degrees C, and the number of degree days above these thresholds required for median transmission (inverse of the slope) was 67.6 and 115.2, respectively. Although the bodies of mos...
Variability of alpha-tocopherol values associated with procurement, storage, and freezing of equine serum and plasma samples. Recent evidence concerning the pathogenesis of equine degenerative myeloencephalopathy indicated that low blood alpha-tocopherol values are a factor in the disease process. Variables that could be introduced by a veterinarian procuring, transporting, or storing samples were evaluated for effects on alpha-tocopherol concentration in equine blood. These variables included temperature; light; exposure to the rubber stopper of the evacuated blood collection tube; hemolysis; duration of freezing time, with and without nitrogen blanketing; and repeated freeze/thaw cycles. It was found that hemolysis...
Microwave thermography: a non-invasive technique for investigation of injury of the superficial digital flexor tendon in the horse. Microwave thermographs were recorded from 77 normal horses. In 51% the lowest temperature was recorded in the mid-metacarpal region, and in 41% it was in the distal metacarpal region. The mean temperature of the normal limbs ranged from 25.04 to 37.4 degrees C. Maximum temperature differences between symmetrical points in both forelimbs ranged from 0 to 5.33 degrees C and differences in mean limb temperatures between both forelimbs ranged from 0 to 2.91 degrees C. In 48 horses with acute (less than 4 weeks' duration) injury of the superficial digital flexor tendon (SDFT) (36 unilateral, 12 bil...
Respiratory muscle perfusion in ponies during prolonged submaximal exercise in thermoneutral environment. Distribution of blood flow among various respiratory muscles was examined in 8 healthy ponies during submaximal exercise lasting 30 minutes, using radionuclide labeled 15-microns diameter microspheres injected into the left ventricle. From the resting values (40 +/- 2 beats/min; 37.3 +/- 0.2 C), heart rate and pulmonary arterial blood temperature increased significantly at 5 (152 +/- 8 beats/min; 38.6 +/- 0.2 C), 15 (169 +/- 6 beats/min; 39.8 +/- 0.2 C), and 26 (186 +/- 8 beats/min; 40.8 +/- 0.2 C) minutes of exertion, and the ponies sweated profusely. Mean aortic pressure also increased progr...
Cardiorespiratory parameters in draught horses before and after short term draught work pulling loads. In order to establish the relationship between draught force and cardiorespiratory responses to exercise heart rate (HR), respiratory rate (RR), arterial and venous blood gases, pH, hemoglobin concentration and temperature were measured in five draught horses during rest, immediately after exercise and 30 min post-exercise under field conditions. A wagon equipped with an odometer and a hydraulic dynamometer was used for measuring distance and draught force. The wagon was loaded with 946 kg for the low load, 1,979 kg for the medium load and 2,994 kg for the high load, and drawn for a distance o...
Hoof and distal limb surface temperature in the normal pony under constant and changing ambient temperatures. Forelimb surface temperatures were continuously monitored in four clinically normal ponies exposed to: (i) constant ambient temperature; (ii) a biphasic change in ambient temperature; and (iii) an incremental increase in ambient temperature. Limb surface temperatures were recorded at the hoof, metacarpus and forearm, and rectal temperature was also measured. Under constant ambient temperature, limb surface temperatures remained relatively constant. A pyrexic episode occurred in one pony under constant ambient temperature conditions and was characterised by an onset phase in which rectal temper...
[The effect of storage time and temperature on the activity of glutathione peroxidase in plasma and whole blood of horses]. The influence of temperature and duration of storage on glutathione peroxidase (GSH-Px) activity in plasma and whole blood samples of horses was investigated. Furthermore the relationships between the GSH-Px activities in plasma and whole blood as well as the GSH-Px values related to hematocrit and the hemoglobin content of the blood samples of 93 different horses were calculated. At 20-22 degrees C, the GSH-Px activity in plasma and whole blood samples remained stable over at least three days while it was reduced by 38% and 65% after 2 and 3 days, if the whole blood samples were kept stored a...
Stability of equine lysozyme. I. Thermal unfolding behaviour. The thermal denaturation of Ca(2+)- and apo-forms of equine lysozyme was followed by using far and near UV circular dichroism and intrinsic fluorescence methods. The difference found between the temperature dependence of the ellipticity at 222 nm and 287 nm, which show two stages in the thermal transition, and those at 228 nm and 294 nm, which indicate only one stage over a wide range of temperatures reflects that different subdivisions of the protein molecule are characterized by a different stability, cooperativity and pathway of denaturation. The first transition, reflected in the increase ...
Blood gas analyses on equine blood: required correction factors [see comment]. Correction factors have been determined to obtain the best estimates of PO2, PCO2 and pH in equine blood with standard blood gas and pH electrodes. There was a significant difference between the PO2 readings for tonometred blood of most horses and the equilibrating gas. Thus, if the PO2 electrode is calibrated with a gas, an electrode correction factor should be obtained by tonometring a blood sample from each horse. This factor was not dependent on packed cell volume. No such correction is required for the PCO2 electrode. If the animal's temperature differs from that of the analyser, the PO2,...
The ammonia tolerance test in horses. Clinically normal horses (n = 8) with ages ranging from 5 to 8 years, were starved for 12 h and their plasma ammonia concentrations were measured. The mean fasting plasma ammonia concentration was 17.8 +/- 3.8 mumol l-1. After dosing ammonium chloride at a dose rate of 0.02 g kg-1, there was a significant increase in plasma ammonia concentration, with a maximum rise after 20 min (P less than 0.05). To investigate the influence of temperature on plasma ammonia concentrations of stored samples, 8 plasma samples were stored at -20 degrees C and 4 degrees C respectively. The plasma ammonia concent...
Vibrational modes of hemoglobin in red blood cells. Equine red blood cells were washed in saline heavy water (2H2O) to exchange the hydrogen atoms of the non-hemoglobin components with deuterons. This led to novel neutron scattering measurements of protein vibrations within a cellular system and permitted a comparison with inelastic neutron scattering measurements on purified horse hemoglobin, either dry or wetted with 2H2O. As a function of wavevector transfer Q and the frequency transfer v the neutron response typified by the dynamic structure factor S(Q, v) was found to be similar for extracted and cellular hemoglobin at low and high tempera...
Thermophilic and thermotolerant fungi of animals’ hair. Nine thermophilic genera and 17 species in addition to one variety of Aspergillus flavus, Malbranchea pulchella and Humicola grisea were collected from hair samples in Riyadh, Saudi Arabia at 45 degrees C. Fifty-one hair specimens of rabbit, sheep, camel and horse were examined for the presence of thermophilic fungi. The most frequent species were Aspergillus fumigatus, Aspergillus niger, Thermoascus aurantiacus and Malbranchea pulchella var. sulfurea. In low frequency, Aspergillus flavus, Aspergillus quadrilineatus, Paecilomyces variotii, Paecilomyces aerugineus, Mucor pusillus and Rhizopus s...