Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
GARDIKAS C, KALLINIKOU M, KALLINIKOS G.The present experiments seem to indicate that horse blood is deficient in both Factors VIII and IX, the concentration of Factor VIII varying from 30 to 60 per cent of the normal human levels, and the concentration of Factor IX varying from 50 to 80 per cent, the corresponding concentrations in 12 normal human males varying from 60 to 150 per cent and from 70 to 150 per cent, respectively.
The finding that the recalcification time of horse plasma was considerably longer than that of normal human plasma is of interest, since it is known that only very low concentrations of Factor VIII or Fact...
Buchanan AM, Davis DC, Pedersen NC, Beaman BL.L-phase (CWD) broth and plate media were used in parallel with conventional microbiological media during a 3-year period for culturing synovial and pleural fluids of animals. Two kinds of recoveries were obtained where parallel conventional methods were negative: (1) parent or normal bacteria, in very low numbers; and (2) Type B CWD variants in equally low numbers. Organisms in group 1 were: Streptococcus zooepidemicus from horses (2x); beta-hemolytic streptococci, Lancefield Gp. G (2x); Staphylococcus aureus; Actinobacillus, and Actinomyces viscosus. Group 2 consisted of Bacteroides sp., Prop...
Rombach N, Stubbs NC, Clayton HM.To determine the gross morphology of the multifidus, longus colli, and longus thoracis muscles in the cervical and cranial thoracic portions of the equine vertebral column. Methods: 15 horse cadavers. Methods: The vertebral column was removed intact from the first cervical vertebra (C1) to the seventh thoracic vertebra (T7). After removing the superficial musculature, detailed anatomic dissections of the multifidus, longus colli, and longus thoracis muscles were performed. Results: The multifidus cervicis muscle consisted of 5 bundles/level arranged in lateral, medial, and deep layers from C2 ...
Tekin N, Wöckener A, Klug E.The effect of a skim milk extender and a glycine-containing extender on sperm motility and acrosome morphology of stallion semen was examined. There was no difference concerning acrosome morphology. After 24 hours of preservation motility of the ejaculates diluted with glycine extender was significantly superior to those handled with skim milk extender. Storage at 5 degrees C in all cases gave better results than storage at room temperature. Skim milk extender is an appropriate diluent when the semen is used for al on the day of its collection, whereas the glycine-containing extender offers th...
Cerasoli I, Cornillie P, Gasthuys F, Gielen I, Schauvliege S.To investigate the anatomy of the ear innervation in horses and develop a new approach for the block of the internal and external pinna and acoustic meatus. Methods: Prospective, descriptive, cadaveric study. Methods: A total of 25 heads and necks from horse cadavers. Methods: Phase I: anatomical dissection and dye deposition on 19 heads. With the head positioned in lateral recumbency, 10 mL methylene blue (1:1 sterile water) for the internal auricular and 2 mL for the great auricular nerve block were injected in 21 ears (seven left, 10 right and two bilaterally). The anatomy of all the ears (...
Keller SA, Valet S, Martens A, Weisse B, Fürst AE, Kümmerle JM. The aim of this study was to evaluate cyclic fatigue behaviour of a new pin with a thread run-out design in comparison with three other types of pins commonly used for equine transfixation pin casting. Methods: Twenty-four pairs of equine cadaveric third metacarpal bones (MC3) equipped with one transfixation pin placed horizontally in the distal metaphysis were tested using a simplified model, mimicking the biomechanical situation of equine transfixation pin casting. A 6.3/8.0-mm Imex Duraface pin with thread run-out design (ITROP) was compared with a 6.1-mm smooth Steinmann pin (SSP), a...
Benoit M, Lingen K, Taddei LM, Heffron BT, Hurt L, Lokanc JA, Lingner K, Cardenas E, Flores S, Mayer D, Pilipiak D, Folker-Calderon D, Negrusz A.Pyrilamine (mepyramine) is an H1-receptor antagonist used in human and veterinary medicine. It has the potential to produce central nervous system effects in horses and therefore may have some impact on an outcome of a horse race. A single oral dose of pyrilamine (300 mg/horse) was given to three animals. Serum samples were collected before drug administration and at 0.25, 0.5, 1, 2, 4, 6, 24, 48, 72, 96, 120, and 144 h, and 7, 8, 9, 10, 11, 12, and 13 days post-administration. Urine samples were collected at 0-1, 1-2, 2-4, 4-6, 24, 48, 72, 96, 120, and 144 h, and 7, 8, 9, 10, 11, 12, 13 days ...
Roels S, Tilmant K, Van Daele A, Van Marck E, Ducatelle R.Melanocytic tumours are a well-known clinical and pathological entity in horses, but further phenotypic characterization of these tumours is lacking. Six melanocytic tumours from five horses (two metastatic and four benign) were examined by Ki67, PCNA and p53 immunostaining, DNA nick end labelling (Tunel) and Feulgen staining. The stainings were evaluated using quantitative image analysis. The resulting parameters of growth fraction (Ki67), S-phase index (PCNA), p53 index, apoptotic index, DNA index, nuclear diameter, ploidy balance, proliferation index (Feulgen) and hyperploidy were analysed....
Woods GL, Hillman RB, Schlafer DH.To evaluate embryo transfer as a possible method to circumvent infertility in mares, embryos from 14 normal and 14 infertile mares were collected three times and examined. Fewer flushes (p less than 0.05) from normal than infertile mares (1/42 vs 9/42) contained only abnormal embryos whereas more flushes (p less than 0.05) from normal than infertile mares contained one or more normal embryos (28/42 vs 8/42). More flushes (p less than 0.05) from normal than infertile mares contained embryos (29/42 vs 17/42). The embryo diameters (mm) at either day-7 or day-8 post ovulation were greater (p less ...
Davies Morel MC, Newcombe JR, Hinchliffe J.Delayed uterine involution is a major cause of early reproductive failure in mares. Involution is affected by mare age, and foaling to covering interval. Involution rates vary between the previously non-gravid horn (PNGH), which recovers the quicker, and the previously gravid horn (PGH). Location of a pregnancy and its likely success may, therefore, be affected by its location relative to the previous pregnancy. This study aimed to determine: (i) the location of concepti in consecutive pregnancies; (ii) whether this varies with mare age or foaling to conception interval; (iii) whether location...
Mohr E, Krzywanek H, Pinkowski W.Monitoring training by measuring blood lactate content is a well-known procedure in sports physiology. Because of the difficulties in standardizing a test procedure in field studies, this method is fraught with considerable problems when applying it to race horses. The aim of the present study was to develop a test procedure which is able to give evidence about the training state of different race horses after nonidentical test-loads. Under almost identical environmental conditions, the level of blood lactate was determined in five trotters which were kept in training. Immediately after the si...
Seyedmousavi S, Guillot J, Arné P, de Hoog GS, Mouton JW, Melchers WJ, Verweij PE.The importance of aspergillosis in humans and various animal species has increased over the last decades. Aspergillus species are found worldwide in humans and in almost all domestic animals and birds as well as in many wild species, causing a wide range of diseases from localized infections to fatal disseminated diseases, as well as allergic responses to inhaled conidia. Some prevalent forms of animal aspergillosis are invasive fatal infections in sea fan corals, stonebrood mummification in honey bees, pulmonary and air sac infection in birds, mycotic abortion and mammary gland infections in ...
P K, M M, S M, Kr P, T D, G W, Ma V, K L.Glanders is a transmissible zoonotic disease caused by Burkholderia mallei that infects equids and humans. No glanders cases in equids were reported so far in Nepal. Methods: Following suspected glanders in animals with clinical signs in different regions in Nepal, serum samples were tested by CFT, ELISA and Luminex® tests. Two horses and a mule tested positive for glanders by all tests, while two other equids only tested positive by ELISA and Luminex®. Analysis of swabs and pus samples by a PCR system targeting B. mallei confirmed the presence of the bacterium in the samples collected from ...
Roth TL, White KL, Thompson DL, Horohov DW.It has been proposed that PGE2 is an important immunosuppressant acting at the fetal-maternal interface during pregnancy. We have previously shown that horse conceptus-conditioned medium suppresses lymphocyte proliferation. This experiment was designed to determine if horse conceptus-derived immunosuppressive activity could be attributed to PGE2 production by the trophoblast tissue. Trophoblast tissue from 21-day-old conceptuses was cut into equal sections and cultured in the presence or absence of the prostaglandin inhibitor, indomethacin. Following culture, immunosuppressive activity and the...
Crumley MN, Hodgson DS, Kreider SE.To measure the effects of tidal volume, ventilatory frequency, and oxygen insufflation flow on the fraction of inspired oxygen in cadaveric horse heads attached to a lung model. Methods: 8 heads of equine cadavers. Methods: Each cadaveric horse head was intubated with a nasotracheal tube that extended into the proximal portion of the trachea. Oxygen was delivered through an oxygen catheter contained within and extending to the tip of the nasotracheal tube. The trachea was connected to the lung model by use of a spiral-wound hose with a sampling adaptor. Eight treatment combinations involving 2...
Peek SF, Borah S, Semrad S, McGuirk S, Slack JA, Patton E, Coombs D, Lien L, Darien BJ.Accurate determination of plasma endotoxin concentration is critical for ex vivo and in vitro cellular and molecular studies of endotoxemia in horses. However, reports are conflicting with respect to anticoagulant, handling, and sample preparation. Objective: The purpose of this study was to determine the effect of blood sample fraction and handling time on measurement of endotoxin concentration in horses. Methods: Whole blood, anticoagulated with 3.8% (0.12 M) sodium citrate (9:1), was collected from 5 healthy horses. Whole blood (WB), platelet-rich plasma (PRP), and platelet-poor plasma (PPP...
Grześkowiak Ł, Riedmüller J, Vahjen W, Zentek J.Storage procedures are known to affect the detectability of toxins in equine and human feces. We assessed the impact of different storage conditions on the detectability of toxins in swine feces. Specimens were inoculated with toxins, 112 ng/g of toxin A (TcdA) and 16 ng/g of toxin B (TcdB) and subjected to the following 3 storage treatments: 4°C, -30°C, repetitive freezing at -30°C and thawing. Toxin determination was assessed at 1, 2, 7, 14, and 21 d with ELISA. A decrease in concentrations of TcdA with time was observed for samples stored at 4°C and repetitive freezing-thawing ( ≤0....
Lauková A, Styková E, Focková V, Maďar M.Nowadays, developed more precisious identification techniques have allowed to validate newer enterococcal species. Among them, the species Enterococcus moraviensis was also validated, at first from surface waters. However, in this study, characteristics and potential to bacteriocin production by the strain E. moraviensis EMo 1-1Nik isolated from buccal mucosa of Slovak warm-blood horse breed has been studied. BLASTn analysis allotted this strain to the species E. moraviensis with percentage identity BLASTn 16S rRNA sequence in the strain up to 100% (99.93% similarity with E. moraviensis NR1139...
Bragulla H, Budras KD, Reilly JD.The fetal development of the white line (Zona alba) in the equine hoof is described. Its specific structure of lamellar and interlamellar horn, which in turn is composed of cap and terminal horn, is formed in the second half of the hoof's fetal development. In equine fetuses with a crown-rump length of less than 550 mm, the hoof capsule lacks a 'characteristic' white line since no borders between stratum medium, stratum internum and sole horn are discernible. In the hoof of an equine fetus with a crown-rump length of 550 mm, a narrow white line has taken shape. Its shallow lamellae are arrange...
Davies HM.The third metacarpal bones (MC3) of racing Thoroughbreds are a common site for bone damage. The midshaft dorsal cortex (DC) of MC3 thickens in response to fast exercise. It is not clear if it changes to a shape and size that limits the peak bone strains to a range associated with normal loading in other species. Objective: To relate the proportionate size of the DC cortex in racehorses exercising at racing speed to surface strains, and test whether the DC reached a plateau that was sustained through subsequent exposures to racing speed exercise. Methods: Standardised lateral MC3 radiographs we...
Wollstein M.The parameningococci of Dopter are culturally indistinguishable from true or normal meningococci, but serologically they exhibit differences as regards agglutination, opsonization, and complement deviation. Because of the variations and irregularities of serum reactions existing among otherwise normal strains of meningococci it does not seem either possible or desirable to separate the parameningococci into a strictly definite class. It appears desirable to consider them as constituting a special strain among meningococci not, however, wholly consistent in itself. The distinctions in serum rea...
Bickhardt K, Carstensen CA.The Reflotron-CK test was evaluated with blood samples of healthy and diseased pigs, sheep, cattle, horses and dogs in relation to the standard CK-NAC test (UV-method). The precision within the series on the day of blood sampling was better than VK = 7.5% (coefficient of variation) with both methods. The day to day precision was estimated by using deep frozen plasma and was in the same order of magnitude with the Reflotron-CK and the UV-method (CV less than 10%). While fresh whole blood of sheep, cattle, horses and dogs respectively should be applied directly onto the dry reagent carrier with ...
Vincze S, Brandenburg AG, Espelage W, Stamm I, Wieler LH, Kopp PA, Lübke-Becker A, Walther B.Increasing numbers of companion animals suffering from infections with methicillin-resistant Staphylococcus aureus (MRSA) have been reported in the recent past. These infections are of particular concern because of the limited treatment options for MRSA and their transferability to humans. Since MRSA lineages isolated from infected companion animals often mirror typical human epidemic strains circulating in the same region, successful strategies to combat MRSA need strong and coordinated efforts from both, the human and the veterinary field according to the "One Health" concept. Hence, to iden...
Elcombe ME, Bellone RR, Magdesian KG, Finno CJ.Equine familial isolated hypoparathyroidism (EFIH) and fragile foal syndrome (FFS) are both fatal recessive conditions reported in Thoroughbred foals. The causal variants for EFIH (RAPGEF5 c.2624C>A; EquCab3.0. chr4: g.54108297G>T) and FFS (PLOD1 c.2032G>A; EquCab3.0, chr2: g.39927817) were recently reported. Prevalence assessment for these variants in a large cohort of samples is needed to provide evidence-based recommendations for genetic testing. Objective: To estimate the frequency of the EFIH and FFS variant alleles in the United States Thoroughbred population between 1988 and 20...
Borchers K, Frölich K, Ludwig H.In blood samples of seven captive equid species from four German zoos EHV-1 specific antibodies were detected in 76% and EHV-4 specific antibodies in 73% of the 55 animals, whereas 93% were tested positive for EHV-2 and EHV-5, respectively. In only one blood sample from a Przewalski's wild horse EHV-4 DNA was amplified by PCR. From seven Przewalski's wild horses EHV-2, and from another one EHV-5 was isolated by cocultivation. The identity of the virus isolates was verified by PCR and restriction enzyme digestion.
Flash ML, Crabb HK, Hitchens PL, Firestone SM, Stevenson MA, Gilkerson JR.The number of horses leaving the Australian Thoroughbred (TB) racing industry each year is of concern to animal welfare advocates, public and regulators. A horse's previous athletic performance is a significant driver of retirement from racing. Racehorse performance can be measured in terms of the total number of starts, duration of racing and prize money earned. This study investigated Australian racing records for the 2005 and 2010 Victorian TB foal crops to identify factors associated with total number of starts, racing career duration, prize money earned and age of last race start-up to th...
Wang X, Asgenbaatar N, Shen Y, Yi M, Zhao B, Ren H, Davshilt T, Ulaangerel T, Wang M, Burenbaatar A, Tian S, Li B, Dugarjav M, Bou G.Proliferation of embryonic fibroblasts under the same cell culture conditions, hinny embryonic fibroblasts (HiEFs) was slower than horse embryonic fibroblast (HEFs), donkey embryonic fibroblasts (DEFs) and mule embryonic fibroblasts (MuEFs). The imprinted genes IGF2 and IGF2R are important for cell proliferation. Therefore, we investigated whether the slower proliferation of HiEFs is related to an aberrant gene expression of IGF2 or its receptors or genes influencing the expression of the IGF2 system. Results: Real-time polymerase chain reaction, immunofluorescence and cell starving experiment...
Yorke EH, Judy CE, Saveraid TC, McGowan CP, Caldwell FJ.Distal border fragments of the navicular bone are increasingly being detected due to the improved capabilities of magnetic resonance imaging (MRI), but their clinical significance remains unclear. The purpose of this retrospective study was to describe the location, size, and frequency of fragments in a cohort of horses presented for MRI of the foot and to compare MRI findings with severity of lameness. Archived MRI studies and medical records were searched from March 2006 to June 2008. Horses were included if a distal border fragment of the navicular bone was visible in MRI scans. Confidence ...
Fisher K, Dodam J, Bailey J, Thombs L, Hodgson D, Bukoski A.To compare PaO2 and PaCO2 in horses recovering from general anesthesia maintained with either apneustic anesthesia ventilation (AAV) or conventional mechanical ventilation (CMV). Methods: Randomized, crossover design. Methods: A total of 10 healthy adult horses from a university-owned herd. Methods: Dorsally recumbent horses were anesthetized with isoflurane in oxygen [inspired oxygen fraction = 0.3 initially, with subsequent titration to maintain PaO2 ≥ 85 mmHg (11.3 kPa)] and ventilated with AAV or CMV according to predefined criteria [10 mL kg-1 tidal volume, PaCO2 40-45 mmHg (5.3-6.0 kP...
