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Topic:Veterinary Research

Veterinary research in horses encompasses the study of diseases, health management, and medical treatments specific to equine species. This field investigates various aspects of horse health, including infectious diseases, metabolic disorders, and musculoskeletal conditions. Researchers focus on understanding the pathophysiology of equine ailments, developing diagnostic tools, and evaluating therapeutic interventions. The study of horse health also involves examining preventive measures such as vaccination protocols and nutritional management to promote overall well-being. This page collects peer-reviewed research studies and scholarly articles that explore the diverse areas of veterinary science related to horses, providing insights into disease mechanisms, treatment strategies, and advancements in equine healthcare.
Dermatophilosis: a threat to livestock production in Ethiopia.
DTW. Deutsche tierarztliche Wochenschrift    June 1, 2000   Volume 107, Issue 4 144-146 
Woldemeskel M.A retrospective study of dermatophilosis was made in livestock (cattle, sheep, horses and goats) of Ethiopia under different situations. The disease was reported for the first time in sheep (8.5%), goats (3.14%) and horses (2.84%) from Ethiopia. The occurrence of the disease in cattle was significantly (p < 0.05) higher in wet (6%) than in dry (3.6%) season and in cross (26.9%) than local (3.2%) breeds. The highest prevalence (35%) was noted in cross breeds kept under poor management in the rainy season. The threat of dermatophilosis to animal production in Ethiopia is indicated. Environmen...
Detection and quantitation of Ehrlichia risticii genomic DNA in infected horses and snails by real-time PCR.
Veterinary parasitology    June 1, 2000   Volume 90, Issue 1-2 129-135 doi: 10.1016/s0304-4017(00)00227-2
Pusterla N, Leutenegger CM, Sigrist B, Chae JS, Lutz H, Madigan JE.A real-time quantitative PCR using the TaqMan fluorogenic detection system (TaqMan PCR) was established for identification of Ehrlichia risticii, the agent of Potomac horse fever (PHF). The TaqMan PCR identified an 85 base pair section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was specific for eight tested E. risticii strains. The TaqMan system identified 10 copies of a cloned section of the 16S rRNA gene of E. risticii. The sensitivity and specificity of the TaqMan PCR were similar to those of conventional nested PCR. The TaqMan PCR was evalua...
Ehrlichia equi infection of horses from Minnesota and Wisconsin: detection of seroconversion and acute disease investigation.
Journal of veterinary internal medicine    June 1, 2000   Volume 14, Issue 3 252-257 doi: 10.1892/0891-6640(2000)014<0252:eiohfm>2.3.co;2
Bullock PM, Ames TR, Robinson RA, Greig B, Mellencamp MA, Dumler JS.Equine granulocytic ehrlichiosis (EGE) is caused by infection with Ehrlichia equi. EGE has been reported primarily in northern California, where E equi is transmitted by the tick Ixodes pacificus. Reports of EGE and the emergence of human granulocytic ehrlichia in Minnesota prompted a seroprevalence study of E equi in horses of Minnesota and Wisconsin. Tick (Ixodes scapularis) endemic areas of Minnesota and Wisconsin were compared to nonendemic regions of Minnesota. Indirect fluorescent antibody was used to detect the presence of serum antibodies to E equi. Serum samples from healthy horses, 3...
Papillomatous pastern dermatitis with spirochetes and Pelodera strongyloides in a Tennessee Walking Horse. Rashmir-Raven AM, Black SS, Rickard LG, Akin M.Papillomatous digital dermatitis is a common disease in cattle. The pastern dermatitis observed in a horse shared many of the gross characteristics of papillomatous digital dermatitis in cattle. Lesions included a mixture of proliferative and erosive changes, with a verrucose appearance in some areas. Microscopic similarities included pseudoepitheliomatous and papillomatous epidermal hyperplasia with hyperkeratosis, spongiosis of the epidermis, and intraepidermal spirochetes. The horse was also concurrently infected with Pelodera strongyloides. Papillomatous digital dermatitis in cattle is ass...
Toxigenic Corynebacterium diphtheriae associated with an equine wound infection. Henricson B, Segarra M, Garvin J, Burns J, Jenkins S, Kim C, Popovic T, Golaz A, Akey B.No abstract available
Ultrastructure of the horse tongue: further observations on the lingual integumentary architecture.
Anatomia, histologia, embryologia    May 23, 2000   Volume 29, Issue 1 37-43 doi: 10.1046/j.1439-0264.2000.00232.x
Pfeiffer CJ, Levin M, Lopes MA.This investigation examined primarily epidermal specializations of the adult horse tongue by light, scanning and transmission electron microscopy. Samples were collected from seven regions of the normal tongue of various breeds of horse. The filiform papillae, present on the dorsal and lateral aspects but not the ventral aspect of the tongue, were short, slender and finger-like structures with variable-shaped terminae. The epidermal thickness and height of dermal ridges were reduced on fungiform and vallate papillae, but tissue architecture and keratinocyte ultrastructure of most of the lingua...
Horse conceptuses secrete insulin-like growth factor-binding protein 3.
Biology of reproduction    May 20, 2000   Volume 62, Issue 6 1804-1811 doi: 10.1095/biolreprod62.6.1804
Herrler A, Pell JM, Allen WR, Beier HM, Stewart F.Insulin-like growth factor-I (IGF-I) promotes early embryonic development in several species. In the rabbit, IGF-I binds to the embryonic coats from Day 3 of development onward by a 38-kDa protein that is probably insulin-like growth factor-binding protein 3 (IGFBP3). In the present study, ligand, Western, and Northern blot analyses were used to demonstrate the presence of IGF-I-binding activity, several immunoreactive IGFBP3 proteins, and IGFBP3 mRNA in horse conceptuses with particularly large amounts of immunoreactive IGFBP3 in the conceptus capsule. In addition, immunoprecipitation of radi...
Pyrrolizidine alkaloid poisoning of horses grazing a native heliotrope (Heliotropium ovalifolium).
Australian veterinary journal    May 17, 2000   Volume 77, Issue 6 401-402 doi: 10.1111/j.1751-0813.1999.tb10318.x
Creeper JH, Mitchell AA, Jubb TF, Colegate SM.No abstract available
Autologous morsellised bone grafting restores uncontained femoral bone defects in knee arthroplasty. An in vivo study in horses.
The Journal of bone and joint surgery. British volume    May 17, 2000   Volume 82, Issue 3 436-444 doi: 10.1302/0301-620x.82b3.9491
van Loon CJ, de Waal Malefijt MC, Buma P, Stolk T, Verdonschot N, Tromp AM, Huiskes R, Barneveld A.The properties of impacted morsellised bone graft (MBG) in revision total knee arthroplasty (TKA) were studied in 12 horses. The left hind metatarsophalangeal joint was replaced by a human TKA. The horses were then randomly divided into graft and control groups. In the graft group, a unicondylar, lateral uncontained defect was created in the third metatarsal bone and reconstructed using autologous MBG before cementing the TKA. In the control group, a cemented TKA was implanted without the bone resection and grafting procedure. After four to eight months, the animals were killed and a biomechan...
Virulence of the V592 isolate of equid herpesvirus-1 in ponies.
Journal of comparative pathology    May 12, 2000   Volume 122, Issue 4 288-297 doi: 10.1053/jcpa.1999.0373
Smith KC, Whitwell KE, Mumford JA, Hannant D, Blunden AS, Tearle JP.The V592 strain of equid herpesvirus-1 (EHV-1), which was originally isolated from a fetus during an abortion epizootic, has proved to be of low virulence in infection studies. Five Welsh Mountain pony mares and one foal were challenged intranasally or by aerosol with this isolate, and monitored clinically and virologically. All six animals shed virus in nasopharyngeal mucus, and viraemia was recorded from day 7 post-infection (PI). Pathological investigations revealed mild rhinitis and bronchiolitis in the mares, with viral antigen expression in degenerating epithelial cells of the nasal muco...
Effects of phenylbutazone on bone activity and formation in horses.
American journal of veterinary research    May 10, 2000   Volume 61, Issue 5 537-543 doi: 10.2460/ajvr.2000.61.537
Rohde C, Anderson DE, Bertone AL, Weisbrode SE.To determine the effects of phenylbutazone (PBZ) on bone activity and bone formation in horses. Methods: 12 healthy 1- to 2-year-old horses. Methods: Biopsy was performed to obtain unicortical bone specimens from 1 tibia on day 0 and from the contralateral tibia on day 14. Fluorochromic markers were administered IV 2 days prior to and on days 0, 10, 15, and 25 after biopsy was performed. Six horses received PBZ (4.4 mg/kg of body weight, PO, q 12 h) and 6 horses were used as controls. All horses were euthanatized on day 30 and tissues from biopsy sites, with adjacent cortical bone, were collec...
Evaluation of the role of keratan sulphate as a molecular marker to monitor cartilage metabolism in horses.
Journal of veterinary medicine. A, Physiology, pathology, clinical medicine    May 10, 2000   Volume 47, Issue 2 99-105 doi: 10.1046/j.1439-0442.2000.00267.x
Okumura M, Fujinaga T.The role of keratan sulphate (KS) as a metabolic marker of cartilage was evaluated using an in vitro model of equine articular cartilage. Articular cartilage was harvested from clinically healthy 6-month-old foals (n = 3). Chondrocytes were centrifuged and cultured as pellets. Chondrocyte pellets were stimulated by insulin-like growth factor-I alpha (IGF-I alpha) or interleukin-1 alpha (IL-1 alpha) for 2 weeks. The concentrations of sulphated glycosaminoglycans (GAG) and KS in the culture media were measured by a 1,9-dimethyl-methylene blue (DMMB) colorimetric assay and an inhibition enzyme-li...
The predicted metal-binding region of the arterivirus helicase protein is involved in subgenomic mRNA synthesis, genome replication, and virion biogenesis.
Journal of virology    May 9, 2000   Volume 74, Issue 11 5213-5223 doi: 10.1128/jvi.74.11.5213-5223.2000
van Dinten LC, van Tol H, Gorbalenya AE, Snijder EJ.Equine arteritis virus (EAV), the prototype Arterivirus, is a positive-stranded RNA virus that expresses its replicase in the form of two large polyproteins of 1,727 and 3,175 amino acids. The functional replicase subunits (nonstructural proteins), which drive EAV genome replication and subgenomic mRNA transcription, are generated by extensive proteolytic processing. Subgenomic mRNA transcription involves an unusual discontinuous step and generates the mRNAs for structural protein expression. Previously, the phenotype of mutant EAV030F, which carries a single replicase point mutation (Ser-2429...
Forced expiration: a test for airflow obstruction in horses.
Journal of applied physiology (Bethesda, Md. : 1985)    May 8, 2000   Volume 88, Issue 5 1870-1879 doi: 10.1152/jappl.2000.88.5.1870
Couëtil LL, Rosenthal FS, Simpson CM.The purpose of this study was to assess whether our method of inducing forced expiration detects small airway obstruction in horses. Parameters derived from forced expiratory flow-volume (FEFV) curves were compared with lung mechanics data obtained during spontaneous breathing in nine healthy horses, in three after histamine challenge, and in two with chronic obstructive pulmonary disease (COPD) pre- and posttherapy with prednisone. Parameters measured in the healthy horses included forced vital capacity (FVC = 41.6 +/- 5.8 liters; means +/- SD) and forced expiratory flow (FEF) at various perc...
Epinephrine inhibits exogenous glucose utilization in exercising horses.
Journal of applied physiology (Bethesda, Md. : 1985)    May 8, 2000   Volume 88, Issue 5 1777-1790 doi: 10.1152/jappl.2000.88.5.1777
Geor RJ, Hinchcliff KW, McCutcheon LJ, Sams RA.This study examined the effects of preexercise glucose administration, with and without epinephrine infusion, on carbohydrate metabolism in horses during exercise. Six horses completed 60 min of treadmill exercise at 55 +/- 1% maximum O(2) uptake 1) 1 h after oral administration of glucose (2 g/kg; G trial); 2) 1 h after oral glucose and with an intravenous infusion of epinephrine (0.2 micromol. kg(-1). min(-1); GE trial) during exercise, and 3) 1 h after water only (F trial). Glucose administration (G and GE) caused hyperinsulinemia and hyperglycemia ( approximately 8 mM). In GE, plasma epine...
Sequence analysis of trinucleotide repeat microsatellites from an enrichment library of the equine genome.
Genome    May 3, 2000   Volume 43, Issue 2 354-365 
Tozaki T, Inoue S, Mashima S, Ohta M, Miura N, Tomita M.Microsatellites are useful tools for the construction of a linkage map and parentage testing of equines, but only a limited number of equine microsatellites have been elucidated. Thus, we constructed the equine genomic library enriched for DNA fragments containing (CAG)n repeats. The enriched method includes hybridization-capture of repeat regions using biotin-conjugated oligonucleotides, nucleotide substrate-biased polymerase reaction with the oligonucleotides and subsequent PCR amplification, because these procedures are useful for the cloning of less abundant trinucleotide microsatellites. ...
Corneal thickness, intraocular pressure, and optical corneal diameter in Rocky Mountain Horses with cornea globosa or clinically normal corneas.
American journal of veterinary research    May 3, 2000   Volume 60, Issue 10 1317-1321 
Ramsey DT, Hauptman JG, Petersen-Jones SM.To compare corneal thickness, intraocular pressure, and optical corneal diameter in Rocky Mountain Horses with cornea globosa and those with clinically normal corneas. Methods: 129 Rocky Mountain Horses. Methods: Ultrasonic pachymetry was used to measure corneal thickness. Applanation tonometry was used to measure intraocular pressure. A Jameson caliper was used to measure optical corneal diameter. Results: The central and temporal peripheral portions of the cornea were significantly thicker in horses with cornea globosa than in horses with clinically normal corneas, but corneal thicknesses in...
Effect of inhaled nitric oxide on experimentally induced pulmonary hypertension in neonatal foals.
American journal of veterinary research    May 3, 2000   Volume 60, Issue 10 1207-1212 
Lester GD, DeMarco VG, Norman WM.To evaluate the efficacy of inhaled nitric oxide (NO) in anesthetized healthy newborn foals with experimentally induced pulmonary hypertension. Methods: Five 1- to 3-day-old foals. Methods: Anesthesia was induced and maintained with propofol, and foals were intubated and mechanically ventilated. Systemic pressure and pulmonary arterial pressure (P(PA)) were recorded every 30 seconds. Hypertension was induced via a hypoxic gas mixture or chemical vasoconstriction, using the thromboxane mimetic U46619. Nitric oxide was added at a concentration of 80 parts per million (ppm) for 6 minutes under ba...
Electrically elicited blink reflex in horses with trigeminal and facial nerve blocks.
American journal of veterinary research    May 3, 2000   Volume 60, Issue 10 1287-1291 
Anor S, Espadaler JM, Monreal L, Pumarola M.To reassess reference values for the components of the electrically induced blink reflex, document reference values for facial motor nerve conduction velocity, and demonstrate usefulness of the blink reflex as a diagnostic tool in peripheral facial and trigeminal nerve dysfunction in horses. Methods: 10 healthy adult horses (8 males, 2 females) without neurologic abnormalities. Methods: Blink reflex tests were performed by electrical stimulation of the supraorbital nerve and facial (auriculopalpebral) nerve. Reflex and direct muscle-evoked potentials of the orbicularis oculi muscles were recor...
Resistance of Rhodococcus equi to acid pH.
International journal of food microbiology    May 3, 2000   Volume 55, Issue 1-3 295-298 doi: 10.1016/s0168-1605(00)00172-0
Benoit S, Taouji S, Benachour A, Hartke A.Rhodococcus equi is an important gram-positive intracellular facultative pathogen in foals of less than 3 months of age, that causes suppurative bronchopneumonia, lymphadenitis and/or enteritis. The disease in young foals mainly occurs in spring and summer when weather conditions are favorable for survival and multiplication of the bacteria in the environment. R. equi is widespread in the environment of horsebreeding farms: it has been isolated from the soil of paddocks and from the feces of adult horses and foals. Aerosol infection via dust of paddocks seems to be the major route of foal infe...
Factors associated with time until first race and career duration for Thoroughbred racehorses.
American journal of veterinary research    May 3, 2000   Volume 60, Issue 10 1196-1200 
Bailey CJ, Reid SW, Hodgson DR, Rose RJ.To determine whether sex or foaling period were associated with time to first race start and whether sex, age at time of first race start, or inter-race period were associated with career duration for Thoroughbred racehorses in Australia. Methods: 553 Thoroughbred racehorses. Methods: Race records through to the end of the 6-year-old racing season were obtained for each horse. Product-limit survival analysis was used to determine whether sex or foaling period was associated with time to first race start and whether sex or age at first race was associated with career duration. A Cox proportiona...
Development of a fluorescence polarization-based diagnostic assay for equine infectious anemia virus.
Journal of clinical microbiology    May 2, 2000   Volume 38, Issue 5 1854-1859 doi: 10.1128/JCM.38.5.1854-1859.2000
Tencza SB, Islam KR, Kalia V, Nasir MS, Jolley ME, Montelaro RC.The control of equine infectious anemia virus (EIAV) infections of horses has been over the past 20 years based primarily on the identification and elimination of seropositive horses, predominantly by a standardized agar gel immunodiffusion (AGID) assay in centralized reference laboratories. This screening for EIAV-seropositive horses has been to date hindered by the lack of a rapid diagnostic format that can be easily employed in the field. We describe here the development of a rapid solution-phase assay for the presence of serum antibodies to EIAV based on fluorescence polarization (FP) (pat...
Osteoclastic resorption of equine cranial and postcranial bone in vitro.
Journal of bone and mineral metabolism    April 28, 2000   Volume 18, Issue 3 148-152 doi: 10.1007/s007740050105
Kingsmill VJ, Gray C, Boyde A.To address possible differences in the resorbability of cranial and postcranial bone, slices of equine frontal bone and leg (first phalanx or third metacarpus) were seeded with embryonic chick bone cells and cultured for 20-24h. After removing the cells and drying the specimens, the areas and volumes of more than 800 resorption pits in each set were measured using a video-rate reflection confocal microscope system. Relative mineralization densities were determined by quantitative electron backscattering analysis. The mean mineralization density was greater in the leg bone, but the mean depths ...
Antimicrobial resistance in bacteria from pets and horses.
Acta veterinaria Scandinavica. Supplementum    April 28, 2000   Volume 92 37-50 
Sternberg S.No abstract available
Twenty-one new equine dinucleotide repeat microsatellites.
Animal genetics    April 27, 2000   Volume 31, Issue 2 141 doi: 10.1046/j.1365-2052.2000.00574.x
Roberts MC, Murtaugh J, Valberg SJ, Mickelson JR, Alexander LJ.No abstract available
Polymorphic microsatellites associated with the equine CKM and CMA1 genes.
Animal genetics    April 27, 2000   Volume 31, Issue 2 141-142 doi: 10.1046/j.1365-2052.2000.00577.x
Caetano AR, Murray JD, Bowling AT.No abstract available
Ten equine dinucleotide microsatellite repeats HTG18-19, HTG22-24, HTG26-27, HGT29-30 and HTG32.
Animal genetics    April 27, 2000   Volume 31, Issue 2 145-146 doi: 10.1046/j.1365-2052.2000.00585.x
Lindgren G.No abstract available
Two polymorphic markers for the horse SLC11A1 (NRAMP1) gene.
Animal genetics    April 27, 2000   Volume 31, Issue 2 152 doi: 10.1046/j.1365-2052.2000.00599.x
Horín P, Matiasovic J.No abstract available
Use of a non-linear spline regression to model time-varying fluctuations in mammary-secretion element concentrations of periparturient mares in Michigan, USA.
Preventive veterinary medicine    April 27, 2000   Volume 43, Issue 3 211-222 doi: 10.1016/s0167-5877(99)00099-9
Lloyd JW, Rook JS, Braselton E, Shea ME.A study was designed to model the fluctuations of nine specific element concentrations in mammary secretions from periparturient mares over time. During the 1992 foaling season, serial samples of mammary secretions were collected from all 18 pregnant Arabian mares at the Michigan State University equine teaching and research center. Non-linear regression techniques were used to model the relationship between element concentration in mammary secretions and days from foaling (which connected two separate sigmoid curves with a spline function); indicator variables were included for mare and mare ...
Scintigraphic characterization of distal radial physeal closure in young Standardbred racehorses. Uhlhorn H, Eksell P, Carlsten J.A longitudinal scintigraphic and radiographic study of distal radial physeal activity in 14 Standardbred trotters was conducted to quantify and visually evaluate physeal uptake around the time of physeal closure. Initial intense 99mTc-HDP uptake was seen, followed by a rapid decrease of activity starting at a mean age of 20 months. Mean physeal to distal diaphyseal ROI and line profile ratios compared to ratios of mature horses decreased from 2.8 to 1.4 at the time of radiographic closure at 24-32 months. Ratios then gradually decreased until values of mature horses were reached at the end of ...