Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Degueurce C.The first veterinary school in the world was created in Lyon in 1761 by an equerry, Claude Bourgelat (1712-1779), a man renowned for his skill in horsemanship and horse medicine, a man fully able to understand the issues of his time. Aware of the scope of the physiocratic movement and of the need to improve the health of farm animals, he was able to understand the expectations of Henri-Léonard Bertin, Minister of King Louis XV, in proposing the creation of an establishment, breaking with traditional farriery. The creation of the Veterinary School of Lyon would mark a milestone: the considerat...
Baus MR, Magnus RP.Purchasing new or used capital assets involves both emotional and financial components in the decision making process. In addition, there are intangible factors such as client perceptions and expectations that fit into the equation. Identify the facts from a business perspective and then define whether this investment (expense) is good for the practice and fits with the short- and long-term growth strategies. Ask the tough questions, analyze the numbers, understand the clients’ needs, and tie this together with the practice brand and strategic objectives. The informed and prepared buyer is u...
Lescun TB, Baird DK, Oliver LJ, Adams SB, Hawkins JF, Moore GE.To determine the extent to which a hydroxyapatite coating promotes pin stability in the third metacarpal bone during transfixation casting in horses. Methods: 14 adult horses. Methods: 7 horses each were assigned to either an uncoated or hydroxyapatite-coated pin group. Three transcortical pins were placed in the third metacarpal bone of each horse and incorporated into a cast for 8 weeks. Insertion and extraction torque were measured, and torque reduction was calculated. Radiography was performed at 0, 4, and 8 weeks. Lameness evaluation was performed at 2, 4, 6, and 8 weeks. Bacteriologic cu...
Cwiklinski K, Kooyman FN, Van Doorn DC, Matthews JB, Hodgkinson JE.Cyathostomins comprise a group of 50 species of parasitic nematodes that infect equids. Ribosomal DNA sequences, in particular the intergenic spacer (IGS) region, have been utilized via several methodologies to identify pre-parasitic stages of the commonest species that affect horses. These methods rely on the availability of accurate sequence information for each species, as well as detailed knowledge of the levels of intra- and inter-specific variation. Here, the IGS DNA region was amplified and sequenced from 10 cyathostomin species for which sequence was not previously available. Also, add...
Lampe JF, Andre J.This study has shown that domestic horses are capable of cross-modal recognition of familiar humans. It was demonstrated that horses are able to discriminate between the voices of a familiar and an unfamiliar human without seeing or smelling them at the same moment. Conversely, they were able to discriminate the same persons when only exposed to their visual and olfactory cues, without being stimulated by their voices. A cross-modal expectancy violation setup was employed; subjects were exposed both to trials with incongruent auditory and visual/olfactory identity cues and trials with congruen...
Caron JP.Laparoscopic techniques for veterinary surgery are growing in popularity for the same reason the use of minimally invasive procedures has increased tremendously in human surgery. Compared with open surgery, the benefits of laparoscopic surgery include smaller incisions, reduced postoperative morbidity and pain, shorter hospitalization, a more rapid return to normal activities, and, in some instances, superior access. Laparoscopic surgery is different from open surgery in equipment/instrumentation and psychomotor skills. This first article in a series on equine laparoscopic surgery introduces t...
Field H, Crameri G, Kung NY, Wang LF.Hendra virus, a novel and fatally zoonotic member of the family Paramyxoviridae, was first described in Australia in 1994. Periodic spillover from its natural host (fruit bats) results in catastrophic disease in horses and occasionally the subsequent infection of humans. Prior to 2011, 14 equine incidents involving seven human cases (four fatal) were recorded. The year 2011 saw a dramatic departure from the sporadic incidents of the previous 16 years, with a cluster of 18 incidents in a single 3-month period. The fundamental difference in 2011 was the total number of incidents, the geographic ...
Hauser H, Richter DC, van Tonder A, Clark L, Preston A.Contagious equine metritis (CEM) is an important venereal disease of horses that is of concern to the thoroughbred industry. Taylorella equigenitalis is a causative agent of CEM but very little is known about it or its close relative Taylorella asinigenitalis. To reveal novel information about Taylorella biology, comparative genomic analyses were undertaken. Whole genome sequencing was performed for the T. equigenitalis type strain, NCTC11184. Draft genome sequences were produced for a second T. equigenitalis strain and for a strain of T. asinigenitalis. These genome sequences were analysed an...
Starke SD, Witte TH, May SA, Pfau T.Gait analysis using small sensor units is becoming increasingly popular in the clinical context. In order to segment continuous movement from a defined point of the stride cycle, knowledge about footfall timings is essential. We evaluated the accuracy and precision of foot contact timings of a defined limb determined using an inertial sensor mounted on the pelvis of ten horses during walk and trot at different speeds and in different directions. Foot contact was estimated from vertical velocity events occurring before maximum sensor roll towards the contralateral limb. Foot contact timings mat...
Proops L, Burden F, Osthaus B.Donkeys and mules are frequently kept as companion animals for horses and ponies, with these different equids often being considered a homogenous group. However, the extent to which domestic equids form inter-specific bonds and display similar social behaviour when living in a mixed herd has not previously been studied. Here we compare the social organization of these three (sub)species when housed together, providing the first systematic analysis of how genetic hybridization is expressed in the social behaviour of mules. A group of 16 mules, donkeys and ponies was observed for 70h and preferr...
Hoffmann G, Bentke A, Rose-Meierhöfer S, Berg W, Mazetti P, Hardarson GH.Horses are often stabled in individual boxes, a method that does not meet their natural needs and may cause psychical and musculoskeletal diseases. This problem is particularly evident in Iceland, where horses often spend the long winter periods in cramped boxes. The aim of this study was to analyze the suitability of a group housing system in Iceland, but the results are also applicable to horses of other regions. Eight Icelandic horses were observed in an active stable system, and their behavior and time budget were recorded. Movement and lying behavior were studied with ALT (Activity, Lying...
Moreau P.A healthy group of loyal clients is of the utmost importance for a healthy practice.Knowing the clients’ expectations and making every effort to exceed them when clients meet with the veterinarian or visit the clinic will result in continued client satisfaction. Clients will show loyalty to a veterinarian when they perceive that the care provided to them suits them and their animal and exceeds their expectations.
Russell T, Pollock PJ.To report a technique for improving surgical access to the bladder for removal of cystic calculi in male horses. Methods: Retrospective case series. Methods: Geldings (8) with cystic calculi. Methods: After catheterization of the bladder through the penis, 100 mL 2% lidocaine hydrochloride solution was instilled. After 10 minutes, the bladder was distended with sterile, warmed Hartmann's solution to a pressure of ≈ 40 cm H(2) O, using gravity feed. This was left in place until abdominal access was gained at surgery, then the fluid siphoned off via the catheter. Results: Calculi were 3-11 cm ...
Mitchell JS.Lameness and performance evaluation can be one of the most rewarding aspects of equine veterinary practice. There is a misconception that it depends on new sophisticated and expensive diagnostic modalities, when the reality is that knowing where and when to use these modalities form the real art of equine lameness practice. The most expensive ultrasound machine in the world is not very diagnostic if applied to the wrong limb. The art of lameness practice is vested in knowledge of horsemanship, an understanding of anatomy and function, and inquiring senses to sort out what the horse and his han...
Vervuert I, Brüssow N, Bochnia M, Cí·¯ord D, Coenen M.The aims of this study were to monitor electromyographic (EMG) activity of masseter muscle in healthy horses fed (i) different types of roughage and (ii) maize after different hay allocations. Four horses were offered the following three diets ad libitum: hay, haylage or straw/alfalfa chaff (SAC). In a second trial, four horses were fed cracked maize (CM) and hay in three different orders: (i) CM after a 12-h overnight fast; (ii) CM immediately after restricted hay intake (0.6 kg hay/100 kg BW); or 3) CM after hay intake ad libitum. The activity of the masseter muscle was determined by EMG...
Tsujita H, Brennan AB, Plummer CE, Nakajima N, Hyon SH, Barrie KP, Sapp B, Jackson D, Brooks DE.Chemically defined bioadhesives (CDB) are "two-component reactive adhesives" made from antibiotic food additives. They contain no animal-derived ingredients and are characterized by a high level of self-degradability, low toxicity, and strong bonding properties. Amniotic membrane (AM) transplantation is used to repair severe ocular surface disease in humans and horses. AM requires meticulous suturing to the injured cornea. The purpose of this study is to determine whether CDB can facilitate the suture-less placement and adherence of equine AM to porcine cadaver cornea. Methods: Prospective stu...
Ohmura H, Hobo S, Hiraga A, Jones JH.To determine the influence of transportation by road and air on heart rate (HR) and HR variability (HRV) in horses. Animals-6 healthy horses. Methods: ECG recordings were obtained from horses before (quarantine with stall rest [Q]; 24 hours) and during a journey that included transportation by road (RT; 4.5 hours), waiting on the ground in an air stall (W; 5.5 hours), and transportation by air (AT; 11 hours); HR was determined, and HRV indices of autonomic nervous activity (low-frequency [LF; 0.01 to 0.07 Hz] and high-frequency [HF; 0.07 to 0.6 Hz] power) were calculated. Results: Mean ± SD H...
Álvarez I, Fernández I, Lorenzo L, Payeras L, Cuervo M, Goyache F.Pedigree information and 179 mtDNA sequences from two endangered Spanish horse breeds, the Asturcón pony (143) and the Mallorquí horse (36), were analysed to asses: (i) the pedigree and molecular maternal genetic diversity of the two breeds; (ii) the concordance between the dam lines recorded in the corresponding studbooks and the mtDNA haplotypes identified; and (iii) to assess the losses of maternal genetic variability occurred from the foundation of the studbooks to present. Up to 50 Asturcón and 18 Mallorquí founder dam lines were identified in the studbooks analysed. Up to 315 Asturc...
Celaya R, Ferreira LM, García U, Rosa García R, Osoro K.As the number of horses is steadily increasing in Cantabrian less-favoured areas, mostly occupied by nutritionally poor heathland communities, the objective of this research was to study and compare diet selection and performance of crossbred horses and beef cattle (Asturiana de los Valles breed) grazing these areas during the summer. A total of 20 cows (seven lactating their calves and thirteen dry) and 20 mares (eight lactating their foals and twelve dry) were managed during 3 years on a heathland area from June to September and October. The proportion of grass (mostly Pseudarrhenatherum lon...
Purzyc H, Kobryńczuk F, Bojarski J.The purpose of this study has been to evaluate the applicability of discriminant function analysis to determine gender dimorphism in Hucul horses, based on morphological indices obtained in different stages of life. A total of 243 horses, divided into six age groups, have been examined in its course. For each horse we have measured 12 metric traits, which were then used to calculate 13 biometric indices commonly used in horse breeding in Poland. These have become the basis for defining functions classifying the animals by gender in each of the six age groups. This study answers the question of...
Hall SJ.An objective and accountable method is needed for deducing the number of registered animals in a breed from registration data. By following the principle that individual breeders register sufficient young females to be certain of having enough replacements for their current breeding stock, the ratios were calculated of the number of adult females in a breed to the number of female registrations, in a given year. Number of breeds considered were 8 cattle, 16 sheep, 8 pigs, 1 goat and 2 equines, all in the United Kingdom or Ireland. This yielded multipliers (4.4 for cattle, 3.3 for sheep, 3.1 fo...
De Marco M, Miraglia N, Peiretti PG, Bergero D.Several studies have reported data on comparisons between two methods: the total collection of feces and the internal markers method. The aim of this study was to assess the apparent digestibility of two concentrates and to compare the apparent digestion coefficients using the total collection of feces and acid-insoluble ash (AIA) as the internal marker method. In 2009, six adult geldings aged between 3 and 11 years, with an average weight per trial of 543, 540 and 542 kg, respectively, were used to determine the apparent digestibility by means of three in vivo digestibility trials on hay, hay...
Vacek JR, Welch RD, Honnas CM.An arthroscopic approach to the palmaroproximal or plantaroproximal pouch of the distal interphalangeal joint was developed in six cadaver limbs and seven limbs of three clinically normal horses. The dorsal aspect of the proximal border and the proximal articular margin of the distal sesamoid (navicular) bone, the palmar aspect of the distal articular margin of the middle phalanx, the collateral sesamoidean ligaments of the distal sesamoid bone, and the joint capsule attachments were readily accessible. Distending the joints with fluid gave access to portions of the articular surface between t...
Lazarus BS, Luu RK, Ruiz-Pérez S, Bezerra WBA, Becerra-Santamaria K, Leung V, Durazo VHL, Jasiuk I, Barbosa JDV, Meyers MA.The horse hoof wall exhibits exceptional impact resistance and fracture control due to its unique hierarchical structure which contains tubular, lamellar, and gradient configurations. In this study, structural characterization of the hoof wall was performed revealing features previously unknown. Prominent among them are tubule bridges, which are imaged and quantified. The hydration-dependent viscoelasticity of the hoof wall is described by a simplified Maxwell-Weichert model with two characteristic relaxation times corresponding to nanoscale and mesoscale features. Creep and relaxation tests r...
Bampidis V, Azimonti G, Bastos ML, Christensen H, Fašmon Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of a tincture from the bark of J. Presl (cinnamon tincture) when used as a sensory additive in feed and water for drinking for all animal species. The product is a water/ethanol ■■■■■ solution, with a dry matter content of approximately 0.9%. The product contains on average 0.344% polyphenols (of which 0.001% are flavonoids) and 0.001% cinnamaldehyde. Methyleugenol was present at the...
Rumpler B, Riha A, Licka T, Kotschwar A, Peham C.Weight boots are commonly used for Icelandic horses to increase the height of the flight arc of the forelimbs in toelt. Objective: To show the influence of weights and toelting speed on the height of the swing phase. Methods: Eight Icelandic horses (mean ± s.d. 12 ± 3 years old, 369 ± 46 kg) were used. Reflecting makers were placed on the dorsal side of each hoof. The motion was collected with a kinematic system (10 cameras, 120 Hz sample rate, 1.3 Mpixels resolution). The horses were ridden in toelt by 2 experienced riders on a treadmill at 2 different speeds (2.96 m/s ± 0.30 and 4.10 m/s...
Lawrence LA, Ott EA, Miller GJ, Poulos PW, Piotrowski G, Asquith RL.Twenty-five pairs of equine third metacarpals (McIII) were collected from horses of varying breeds and ranging in age from 1 d to 27 yr. Standard three-point bending tests were performed on the McIII to obtain comparative data on breaking load (BL), breaking strength (BS), and elasticity (E). Broken bones were reassembled and 2-cm sections cut from the bone 1 cm below the nutrient foramen and at the site of the break. Bone mineral content (BMC) and geometrical properties of these sections were determined. Maximum BMC (17.3 g/2 cm) was reached at 6.0 +/- 1.8 yr of age. Horses in this study achi...
Fassil H, El Harrak M, Marié JL.Morocco has undergone three outbreaks of West Nile fever. The first, in 1996, began with a case in a horse herd in Benslimane (central region) and spread to neighboring cities (in the central and northwestern regions). The next two outbreaks appeared after identical 7-year epidemiological latency periods (in 2003 and 2010) in the same area and season. The only human case, which was fatal, occurred during the 1996 outbreak. The West Nile virus strains circulating in Morocco are closely related to other western Mediterranean strains and are characterized by high virulence in horses.
Bertram TA, Coignoul FL.Equine neutrophils were evaluated ultrastructurally and by morphometric analysis. Homogeneous populations of neutrophils were isolated from peripheral blood at 4 degrees and 22 degrees C by centrifugation on two sequential Ficoll-Hypaque density gradients. Isolation procedures at both temperatures resulted in neutrophil degranulation but not cell swelling. Degranulation was more extensive in cells isolated at 22 degrees C. Isolation temperature affected the neutrophil content of secondary granules more than primary granules. A granule similar to immature specific granules of human neutrophils ...
Gao R, Liu J, Jing S, Mao W, He P, Liu B, Yang HD, Cao J.Many challenges are encountered in both teaching and learning veterinary obstetrics. This may be due to outdated teaching materials, as the main model of content transmission remains centred around text and images. Methods: Visualisation methods such as three-dimensional (3D) and Graphics Interchange Format (GIF) tools were applied in an attempt to improve obstetrics education outcomes in the third-year class. Traditional teaching methods were utilised in the fourth-year and fifth-year students. Results: These supplementary tools significantly increased the third-year students' final examinati...
Fletcher WO, Stallknecht DE, Jenney EW.Seventeen species of mammals and seven species of birds from Ossabaw Island, Georgia, were tested for vesicular stomatitis (VS) neutralizing antibodies. Seropositive results were restricted to mammals with six of 17 species testing seropositive for VS (New Jersey type) neutralizing antibodies. Seropositive species included: raccoons (Procyon lotor), white-tailed deer (Odocoileus virginianus), feral swine (Sus scrofa), cattle (Bos taurus), horses (Equus caballus), and donkeys (Equus asinus). All tests for VS (Indiana type) were negative.
Smith RM, Bhoora RV, Kotzé A, Grobler JP, Lee Dalton D.Translocation of animals in fragmented habitats is an important means of dispersal and gene flow, however, the movement of animals has led to the spread of various diseases globally and wildlife are often the reservoirs of these diseases. Currently, Cape mountain zebra are translocated within South Africa as a management method for augmentation of isolated and fragmented populations. The movement of pathogens due to translocations in local regions have gone largely unchecked, particularly where there may still be isolated regions that can be negatively affected. Equine piroplasmosis is a tick-...
Gavriliuc S, Reza S, Jeong C, Getachew F, McLoughlin PD, Poissant J.The development of high-throughput sequencing has prompted a transition in wildlife genetics from using microsatellites toward sets of single nucleotide polymorphisms (SNPs). However, genotyping large numbers of targeted SNPs using non-invasive samples remains challenging due to relatively large DNA input requirements. Recently, target enrichment has emerged as a promising approach requiring little template DNA. We assessed the efficacy of Tecan Genomics' Allegro Targeted Genotyping (ATG) for generating genome-wide SNP data in feral horses using DNA isolated from fecal swabs. Total and host-sp...
Hoyt DF, Molinari M, Wickler SJ, Cogger EA.The objective was to understand how the stance-phase kinematics of the hindlimb of trotting horses change with speed under 3 conditions (level, loaded and incline), to compare our results with the predictions of the spring-mass model and to help focus our future studies of muscle function. Video recordings were made of 5 Arabian horses trotting on a treadmill. Five consecutive strides were digitised and averaged for each trial. The angle-time diagrams were qualitatively similar to those reported previously. As speed increases, the range of motion of the hindlimb increases, as predicted by the ...
Marzok M, Al-Jabr OA, Salem M, Alkashif K, Sayed-Ahmed M, Wakid MH, Kandeel M, Selim A. is classified as intracellular protozoa and is one of the major zoonotic parasites. Most warm-blooded intermediate hosts, including humans, are commonly infected by this parasite. The epidemiology of infection in Egyptian horses is currently poorly understood. Methods: 420 blood samples were randomly collected from horses raised in four governorates in Northern Egypt (110 each from Giza and Kafr El Sheikh, and 100 each from Qalyubia and Gharbia) to investigate the existence of antibodies against using a commercial ELISA kit, and to ascertain the risk factors for the infection. Results: the ...
Rochais C, Lerch N, Gueguen L, Schmidlin M, Bonamy O, Grandgeorge M, Hausberger M.Tactile perception in humans varies between individuals and could depend on extrinsic factors such as working activity. In animals, there is no study relating the influence of animals' work and their tactile reactivity . We investigated horses' tactile reactivity using von Frey filament in different body areas and compared horses working only in equine-assisted interventions (EAI), in riding school (RS) lessons, and in both activities (EAI-RS). We further compared tactile actions by people with or without mental and/or developmental disorders during brushing sessions. The results indicated tha...
Sloet van Oldruitenborgh-Oosterbaan MM, Blok MB, Begeman L, Kamphuis MC, Lameris MC, Spierenburg AJ, Lashley MJ.'Rollkur' or 'overbending' is the low and deep riding of a dressage horse during training or warming up. Lately, this technique has been criticized, and not necessarily objectively, on welfare grounds. To be able to evaluate these criticisms, more needs to be known about the workload and stress of horses being ridden 'rollkur'. The aim of the present study was to compare the workload of eight riding-school horses when being ridden deep and round with a draw rein ('rollkur') and when being ridden in a natural frame with only light rein contact ('free'). Workload (as measured by heart rate and b...
Gaitskell-Phillips G, Martín-Cano FE, da Silva-Álvarez E, Tapia JA, Silva A, Gil MC, Ortega-Ferrusola C, Peña FJ.Although recent research has addressed the impact of cryopreservation on the stallion sperm proteome, studies addressing the stallion sperm phosphoproteome are lacking. In the present study, the data set of proteomes of fresh and cryopreserved spermatozoa were reanalyzed, showing that cryopreservation caused significant changes in the phosphoproteome. The phosphoproteins reduced most significantly by cryopreservation were Ca2+binding tyrosine phosphorylation regulated, protein kinase cAMP-activated catalytic subunit beta (CABYR), mitochondria eating protein (SPATA18), A kinase anchoring protei...
Kaps S, Richter M, Spiess BM.To determine corneal sensitivity in healthy adult horses in order to establish reference values. Methods: One hundred eyes of 50 healthy adult horses. Methods: Corneal sensitivity was determined by evaluating the corneal touch threshold (CTT) in five different corneal regions using a Cochet-Bonnet esthesiometer. Results: Comparing CTT values (in mm filament length) of the five prescribed corneal regions revealed regional variations in corneal sensitivity, with the central region (21.15 +/- 6.23 mm) being the most sensitive, followed by the nasal (20.75 +/- 5.14 mm), temporal (20.70 +/- 5.37 mm...
Franz S, Baumgartner W.Oesophageal endoscopy in cattle has been rarely reported in the literature. It has, however, been used for many years as an aid to the diagnosis and treatment of illnesses of the upper gastrointestinal tract in small animals and horses. We have assessed the potential value of oesophagoscopy on 120 bovine patients presented at the 2nd Medical Clinic for Ruminants and Swine at the University of Veterinary Medicine, Vienna, over a period of three years. In the course of the study, cattle exhibiting symptoms such as salivation, regurgitation or swelling in the neck region were referred for endosco...
Anielski P, Thieme D, Schlupp A, Grosse J, Ellendorff F, Mueller RK.Growing interest among several horse-breeder associations has initiated the development of a screening procedure to test for anabolic agents in hair, which has the advantage over blood and urine specimens of allowing long-term detection. An analytical method was established to monitor in tails or manes several anabolic substances available as veterinary medicines or as so-called nutritional supplements (clenbuterol, different esters or prohormones of nandrolone and testosterone). The analytical procedure to detect steroids in hair samples consists of the following steps: decontamination of the...
Haase B, Jude R, Brooks SA, Leeb T.The tobiano white-spotting pattern is one of several known depigmentation phenotypes in horses and is desired by many horse breeders and owners. The tobiano spotting phenotype is inherited as an autosomal dominant trait. Horses that are heterozygous or homozygous for the tobiano allele (To) are phenotypically indistinguishable. A SNP associated with To had previously been identified in intron 13 of the equine KIT gene and was used for an indirect gene test. The test was useful in several horse breeds. However, genotyping this sequence variant in the Lewitzer horse breed revealed that 14% of ho...
Colahan PT, Jackson CA, Rice B, Szabo N, Jones JH.Sildenafil, a phosphodiesterase-5 inhibitor vasodilator, increases cGMP concentrations by inhibiting enzymatic degradation. Marketed to treat erectile dysfunction in men, it also reduces pulmonary arterial pressure (PAP). Because it reduces PAP, sildenafil may enhance performance and/or prevent exercise induced-pulmonary haemorrhage (EIPH). Objective: To determine if sildenafil citrate administration altered commonly measured indices of performance or reduced EIPH in exercised horses. Methods: Thirteen athletically conditioned Thoroughbred horses (2 mares and 11 geldings, age 3-12 years) were ...
Morris DD, Henry MM, Moore JN, Fischer JK.A study was conducted to determine whether dietary supplements with alpha-linolenic acid altered the ability of equine peritoneal macrophages to produce tumor necrosis factor (TNF) in response to endotoxin. Peritoneal macrophages were harvested from 6 healthy adult horses before and after the horses were fed a nutritionally balanced ration that contained 8% linseed oil as a source of alpha-linolenic acid. The macrophages were cultured in media containing no additives (control), endotoxin (0.5 to 50 ng/ml), or the calcium ionophore, A23187. Macrophage supernatants were collected after 6 and 24 ...
Ohmura H, Mukai K, Takahashi T, Aida H, Jones JH.OBJECTIVE To determine cardiorespiratory responses of Thoroughbreds to uphill and downhill locomotion on a treadmill at identical gradients. ANIMALS 5 highly trained Thoroughbred geldings. PROCEDURES Thoroughbreds were exercised for 2-minute intervals on a treadmill at 1.7, 3.5, 6.0, 8.0, and 10.0 m/s at a 4% incline, 0% incline (horizontal plane), and 4% decline in random order on different days. Stride frequency, stride length, and cardiopulmonary and O-transport variables were measured and analyzed by means of repeated-measures ANOVA and Holm-Šidák pairwise comparisons. RESULTS Horses com...
The Journal of physiologyAugust 1, 1971
Volume 216, Issue 3 659-682 doi: 10.1113/jphysiol.1971.sp009546
Comline RS, Silver M.1. The content and output of adrenaline and noradrenaline from the equine adrenal medulla has been investigated under different conditions in foetuses, foals and adult mares.2. In the foetus only small amounts of both amines were secreted in response to stimulation of the peripheral ends of the splanchnic nerves to the gland; during anoxia the adrenal discharge was far greater and was independent of any nervous mechanism.3. Whereas in the ruminant a direct adrenal response to low P(O2) is confined to the noradrenaline cells during foetal life only, the adrenal medulla of the foetal foal secret...
De-Simone SG, Nascimento HJ, Prado IC, Aguiar AS, Melgarejo AR, Pina JLS, Ferreira PF, Provance DW.The availability of purified antibodies is a prerequisite for many applications and the appropriate choice(s) for antibody-purification is crucial. Numerous methods have been developed for the purification of antibodies from different sources with affinity chromatography-based methods being the most extensively utilized. These methods are based on high specificity, easy reversibility and biological interactions between two molecules (e.g., between receptor and ligand or antibody and antigen). However, no simple techniques have yet been described to characterize and purify subclasses of immunog...
