FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet Immunol Immunopathol / Isolation and immunophenotypic characterization of mesenchym...
Veterinary immunology and immunopathology2009; 132(2-4); 303-306; doi: 10.1016/j.vetimm.2009.06.014

Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.

Abstract: The purpose of this work was to isolate and cultivate mesenchymal stem cells (MSC) derived from equine adipose tissue and conduct cellular characterization with the following markers: CD90, CD44 and CD13. Adipose tissue collection was performed at the base of the horses' tails, followed by immediate isolation and cultivation of the MSC and posterior characterization by flow cytometry for the interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), fluorescein isothiocyanate (FITC), and tests with specific mAb mouse anti-horse CD13 and mouse anti-horse CD44. The technique used for isolation and cell cultivation proved to be safe and viable. The CD90 mAb expressed cross-reaction with MSC derived from equine adipose tissue and CD44 showed greater expression in cells as the number of culture passages increased. Although marker CD13 expresses reaction in other studies involving MSC in different species, it presented no expression in the experiment realized. The results obtained revealed the immunophenotypic characterization of the surface of isolated and cultivated MSC, classifying these cells as a promising type of progenitor cells that can be applied in equine cellular therapy.
Get Full Text
Publication Date: 2009-06-30 PubMed ID: 19647331DOI: 10.1016/j.vetimm.2009.06.014Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is aimed towards the isolation, cultivation, and characterization of mesenchymal stem cells (MSC) from horse fat tissue. The study used specific markers and observed their reactions. The cultivation technique proved effective and safe, and the researchers were able to characterize the isolated stem cells, suggesting potential use in horse cell therapy.

Isolation and Cultivation of MSC from Equine Adipose Tissue

  • Horse fat cells were collected from horses’ tail bases for the purpose of isolating and developing mesenchymal stem cells (MSC).
  • After the collection, the immediate isolation and cultivation of the MSC were undertaken.
  • The isolation and cell cultivation methods observed proved to be safe, yielding viable cells showing that the techniques used are not harmful and effectively produces the desired cells.

Use of Markers for Cellular Characterization

  • The markers used for cellular characterization were CD90, CD44, and CD13, which are common in stem cell research.
  • The tests conducted were an interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), a fluorescent marker fluorescein isothiocyanate (FITC), and specific mAb tests with mouse anti-horse CD13 and mouse anti-horse CD44.
  • It was observed that CD90 mAb showed cross-reaction with equine adipose tissue-derived MSC. This implies that this marker reacts with different species’ MSC, validating its use in MSC research.
  • CD44 showed a higher expression in cells as the number of culture passages (cycles) increased. Every cycle allows the cells to multiply, which could have caused increased expression of this marker.
  • The CD13 marker didn’t show any expression contrary to other studies where CD13 often presents reactions involving MSC in different species. This suggests that equine MSC might have unique characteristics when compared to other species.

Potential Application and Conclusion

  • The study was able to successfully isolate, cultivate, and characterize MSC from horse adipose tissue.
  • Based on their findings, the research concludes that these particular stem cells have a promising future as progenitor cells (cells that create other types of cells) and could be applied in horse cell therapy.
  • This could potentially lead to developing new treatment methods for horses.

Cite This Article

APA
de Mattos Carvalho A, Alves AL, Golim MA, Moroz A, Hussni CA, de Oliveira PG, Deffune E. (2009). Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue. Vet Immunol Immunopathol, 132(2-4), 303-306. https://doi.org/10.1016/j.vetimm.2009.06.014

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 132
Issue: 2-4
Pages: 303-306

Researcher Affiliations

de Mattos Carvalho, Armando
  • Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil. armandomc@fmvz.unesp.br
Alves, Ana Liz Garcia
    Golim, Marjorie Assis
      Moroz, Andrei
        Hussni, Carlos Alberto
          de Oliveira, Patrícia Galvão Gomes
            Deffune, Elenice

              MeSH Terms

              • Adipose Tissue / cytology
              • Adipose Tissue / immunology
              • Animals
              • Antibodies, Monoclonal
              • CD13 Antigens / metabolism
              • Cell Separation
              • Flow Cytometry
              • Fluorescein-5-isothiocyanate
              • Fluorescent Dyes
              • Horses / anatomy & histology
              • Horses / immunology
              • Hyaluronan Receptors / metabolism
              • Immunophenotyping
              • Mesenchymal Stem Cells / classification
              • Mesenchymal Stem Cells / cytology
              • Mesenchymal Stem Cells / immunology
              • Mice
              • Rats
              • Thy-1 Antigens / metabolism

              Citations

              This article has been cited 36 times.
              1. Xu D, Gong Y, Xiang X, Liu Y, Mai K, Ai Q. Discovery, characterization, and adipocyte differentiation regulation in perirenal adipose tissue of large yellow croaker (Larimichthys crocea).. Fish Physiol Biochem 2023 Aug;49(4):627-639.
                doi: 10.1007/s10695-023-01208-2pubmed: 37341909google scholar: lookup
              2. Heilen LB, Roßgardt J, Dern-Wieloch J, Vogelsberg J, Staszyk C. Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament.. Front Vet Sci 2023;10:1116671.
                doi: 10.3389/fvets.2023.1116671pubmed: 36968463google scholar: lookup
              3. Ferreira-Baptista C, Queirós A, Ferreira R, Fernandes MH, Colaço B, Gomes PS. The Osteogenic Potential of Falciform Ligament-Derived Stromal Cells-A Comparative Analysis between Two Osteogenic Induction Programs.. Bioengineering (Basel) 2022 Dec 15;9(12).
                doi: 10.3390/bioengineering9120810pubmed: 36551016google scholar: lookup
              4. de Souza JB, Rosa GDS, Rossi MC, Stievani FC, Pfeifer JPH, Krieck AMT, Bovolato ALC, Fonseca-Alves CE, Borrás VA, Alves ALG. In Vitro Biological Performance of Alginate Hydrogel Capsules for Stem Cell Delivery.. Front Bioeng Biotechnol 2021;9:674581.
                doi: 10.3389/fbioe.2021.674581pubmed: 34513806google scholar: lookup
              5. Fülber J, Agreste FR, Seidel SRT, Sotelo EDP, Barbosa ÂP, Michelacci YM, Baccarin RYA. Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system.. World J Stem Cells 2021 Jun 26;13(6):645-658.
                doi: 10.4252/wjsc.v13.i6.645pubmed: 34249233google scholar: lookup
              6. Wright A, Arthaud-Day ML, Weiss ML. Therapeutic Use of Mesenchymal Stromal Cells: The Need for Inclusive Characterization Guidelines to Accommodate All Tissue Sources and Species.. Front Cell Dev Biol 2021;9:632717.
                doi: 10.3389/fcell.2021.632717pubmed: 33665190google scholar: lookup
              7. MacDonald ES, Barrett JG. The Potential of Mesenchymal Stem Cells to Treat Systemic Inflammation in Horses.. Front Vet Sci 2019;6:507.
                doi: 10.3389/fvets.2019.00507pubmed: 32039250google scholar: lookup
              8. Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders.. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
                doi: 10.1007/s12015-019-09932-0pubmed: 31797146google scholar: lookup
              9. Costa CRM, Feitosa MLT, Rocha AR, Bezerra DO, Leite YKC, Argolo Neto NM, Rodrigues HWS, Júnior AS, Silva AS, Sarmento JLR, Silva LS, Carvalho MAM. Adipose stem cells in reparative goat mastitis mammary gland.. PLoS One 2019;14(10):e0223751.
                doi: 10.1371/journal.pone.0223751pubmed: 31639137google scholar: lookup
              10. Yang Q, Pinto VMR, Duan W, Paxton EE, Dessauer JH, Ryan W, Lopez MJ. In vitro Characteristics of Heterogeneous Equine Hoof Progenitor Cell Isolates.. Front Bioeng Biotechnol 2019;7:155.
                doi: 10.3389/fbioe.2019.00155pubmed: 31355191google scholar: lookup
              11. Felix RG, Bovolato ALC, Cotrim OS, Leão PDS, Batah SS, Golim MA, Velosa AP, Teodoro W, Martins V, Cruz FF, Deffune E, Fabro AT, Capelozzi VL. Adipose-derived stem cells and adipose-derived stem cell-conditioned medium modulate in situ imbalance between collagen I- and collagen V-mediated IL-17 immune response recovering bleomycin pulmonary fibrosis.. Histol Histopathol 2020 Mar;35(3):289-301.
                doi: 10.14670/HH-18-152pubmed: 31318036google scholar: lookup
              12. Cortés-Araya Y, Amilon K, Rink BE, Black G, Lisowski Z, Donadeu FX, Esteves CL. Comparison of Antibacterial and Immunological Properties of Mesenchymal Stem/Stromal Cells from Equine Bone Marrow, Endometrium, and Adipose Tissue.. Stem Cells Dev 2018 Nov 1;27(21):1518-1525.
                doi: 10.1089/scd.2017.0241pubmed: 30044182google scholar: lookup
              13. Rink BE, Beyer T, French HM, Watson E, Aurich C, Donadeu FX. The Fate of Autologous Endometrial Mesenchymal Stromal Cells After Application in the Healthy Equine Uterus.. Stem Cells Dev 2018 Aug 1;27(15):1046-1052.
                doi: 10.1089/scd.2018.0056pubmed: 29790424google scholar: lookup
              14. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know.. Front Vet Sci 2018;5:70.
                doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
              15. Angelone M, Conti V, Biacca C, Battaglia B, Pecorari L, Piana F, Gnudi G, Leonardi F, Ramoni R, Basini G, Dotti S, Renzi S, Ferrari M, Grolli S. The Contribution of Adipose Tissue-Derived Mesenchymal Stem Cells and Platelet-Rich Plasma to the Treatment of Chronic Equine Laminitis: A Proof of Concept.. Int J Mol Sci 2017 Oct 11;18(10).
                doi: 10.3390/ijms18102122pubmed: 29019941google scholar: lookup
              16. Fülber J, Maria DA, da Silva LC, Massoco CO, Agreste F, Baccarin RY. Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment.. Stem Cell Res Ther 2016 Mar 5;7:35.
                doi: 10.1186/s13287-016-0294-3pubmed: 26944403google scholar: lookup
              17. Sullivan MO, Gordon-Evans WJ, Fredericks LP, Kiefer K, Conzemius MG, Griffon DJ. Comparison of Mesenchymal Stem Cell Surface Markers from Bone Marrow Aspirates and Adipose Stromal Vascular Fraction Sites.. Front Vet Sci 2015;2:82.
                doi: 10.3389/fvets.2015.00082pubmed: 26835460google scholar: lookup
              18. Lombana KG, Goodrich LR, Phillips JN, Kisiday JD, Ruple-Czerniak A, McIlwraith CW. An Investigation of Equine Mesenchymal Stem Cell Characteristics from Different Harvest Sites: More Similar Than Not.. Front Vet Sci 2015;2:67.
                doi: 10.3389/fvets.2015.00067pubmed: 26664993google scholar: lookup
              19. Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.. PLoS One 2015;10(4):e0122954.
                doi: 10.1371/journal.pone.0122954pubmed: 25902064google scholar: lookup
              20. Maia L, da Cruz Landim-Alvarenga F, Taffarel MO, de Moraes CN, Machado GF, Melo GD, Amorim RM. Feasibility and safety of intrathecal transplantation of autologous bone marrow mesenchymal stem cells in horses.. BMC Vet Res 2015 Mar 15;11:63.
                doi: 10.1186/s12917-015-0361-5pubmed: 25879519google scholar: lookup
              21. Alipour F, Parham A, Kazemi Mehrjerdi H, Dehghani H. Equine adipose-derived mesenchymal stem cells: phenotype and growth characteristics, gene expression profile and differentiation potentials.. Cell J 2015 Winter;16(4):456-65.
                doi: 10.22074/cellj.2015.491pubmed: 25685736google scholar: lookup
              22. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar S, Kumar P, Yadav PS. Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood.. Cytotechnology 2016 Aug;68(4):795-807.
                doi: 10.1007/s10616-014-9831-zpubmed: 25487085google scholar: lookup
              23. Arzi B, Kol A, Murphy B, Walker NJ, Wood JA, Clark K, Verstraete FJ, Borjesson DL. Feline foamy virus adversely affects feline mesenchymal stem cell culture and expansion: implications for animal model development.. Stem Cells Dev 2015 Apr 1;24(7):814-23.
                doi: 10.1089/scd.2014.0317pubmed: 25404388google scholar: lookup
              24. Radtke CL, Nino-Fong R, Esparza Gonzalez BP, McD￮ LA. Application of a novel sorting system for equine mesenchymal stem cells (MSCs).. Can J Vet Res 2014 Oct;78(4):290-6.
                pubmed: 25355998
              25. Zhang N, Dietrich MA, Lopez MJ. Therapeutic doses of multipotent stromal cells from minimal adipose tissue.. Stem Cell Rev Rep 2014 Aug;10(4):600-11.
                doi: 10.1007/s12015-014-9508-1pubmed: 24850472google scholar: lookup
              26. Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
                doi: 10.1186/scrt414pubmed: 24559797google scholar: lookup
              27. Lopez MJ. Bench to bedside: it's all about the model.. Stem Cell Res Ther 2014 Jan 20;5(1):11.
                doi: 10.1186/scrt400pubmed: 24444327google scholar: lookup
              28. Mohanty N, Gulati BR, Kumar R, Gera S, Kumar P, Somasundaram RK, Kumar S. Immunophenotypic characterization and tenogenic differentiation of mesenchymal stromal cells isolated from equine umbilical cord blood.. In Vitro Cell Dev Biol Anim 2014 Jun;50(6):538-48.
                doi: 10.1007/s11626-013-9729-7pubmed: 24414976google scholar: lookup
              29. Reza AM, Shiwani S, Singh NK, Lohakare JD, Lee SJ, Jeong DK, Han JY, Rengaraj D, Lee BW. Keratinocyte growth factor and thiazolidinediones and linolenic acid differentiate characterized mammary fat pad adipose stem cells isolated from prepubertal Korean black goat to epithelial and adipogenic lineage.. In Vitro Cell Dev Biol Anim 2014 Mar;50(3):194-206.
                doi: 10.1007/s11626-013-9690-5pubmed: 24101555google scholar: lookup
              30. Carvalho Ade M, Badial PR, Álvarez LE, Yamada AL, Borges AS, Deffune E, Hussni CA, Garcia Alves AL. Equine tendonitis therapy using mesenchymal stem cells and platelet concentrates: a randomized controlled trial.. Stem Cell Res Ther 2013 Jul 22;4(4):85.
                doi: 10.1186/scrt236pubmed: 23876512google scholar: lookup
              31. Seo MS, Park SB, Kim HS, Kang JG, Chae JS, Kang KS. Isolation and characterization of equine amniotic membrane-derived mesenchymal stem cells.. J Vet Sci 2013;14(2):151-9.
                doi: 10.4142/jvs.2013.14.2.151pubmed: 23388430google scholar: lookup
              32. Ranera B, Remacha AR, Álvarez-Arguedas S, Romero A, Vázquez FJ, Zaragoza P, Martín-Burriel I, Rodellar C. Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue.. BMC Vet Res 2012 Aug 22;8:142.
                doi: 10.1186/1746-6148-8-142pubmed: 22913590google scholar: lookup
              33. Requicha JF, Viegas CA, Albuquerque CM, Azevedo JM, Reis RL, Gomes ME. Effect of anatomical origin and cell passage number on the stemness and osteogenic differentiation potential of canine adipose-derived stem cells.. Stem Cell Rev Rep 2012 Dec;8(4):1211-22.
                doi: 10.1007/s12015-012-9397-0pubmed: 22773405google scholar: lookup
              34. Iacono E, Cunto M, Zambelli D, Ricci F, Tazzari PL, Merlo B. Could fetal fluid and membranes be an alternative source for mesenchymal stem cells (MSCs) in the feline species? A preliminary study.. Vet Res Commun 2012 Jun;36(2):107-18.
                doi: 10.1007/s11259-012-9520-3pubmed: 22327440google scholar: lookup
              35. Mensing N, Gasse H, Hambruch N, Haeger JD, Pfarrer C, Staszyk C. Isolation and characterization of multipotent mesenchymal stromal cells from the gingiva and the periodontal ligament of the horse.. BMC Vet Res 2011 Aug 2;7:42.
                doi: 10.1186/1746-6148-7-42pubmed: 21810270google scholar: lookup
              36. Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells.. Vet Res Commun 2011 Aug;35(6):355-65.
                doi: 10.1007/s11259-011-9480-zpubmed: 21614641google scholar: lookup
              Subscribe to our newsletter
              Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.