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Veterinary immunology and immunopathology2009; 132(2-4); 303-306; doi: 10.1016/j.vetimm.2009.06.014

Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue.

Abstract: The purpose of this work was to isolate and cultivate mesenchymal stem cells (MSC) derived from equine adipose tissue and conduct cellular characterization with the following markers: CD90, CD44 and CD13. Adipose tissue collection was performed at the base of the horses' tails, followed by immediate isolation and cultivation of the MSC and posterior characterization by flow cytometry for the interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), fluorescein isothiocyanate (FITC), and tests with specific mAb mouse anti-horse CD13 and mouse anti-horse CD44. The technique used for isolation and cell cultivation proved to be safe and viable. The CD90 mAb expressed cross-reaction with MSC derived from equine adipose tissue and CD44 showed greater expression in cells as the number of culture passages increased. Although marker CD13 expresses reaction in other studies involving MSC in different species, it presented no expression in the experiment realized. The results obtained revealed the immunophenotypic characterization of the surface of isolated and cultivated MSC, classifying these cells as a promising type of progenitor cells that can be applied in equine cellular therapy.
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Publication Date: 2009-06-30 PubMed ID: 19647331DOI: 10.1016/j.vetimm.2009.06.014Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research is aimed towards the isolation, cultivation, and characterization of mesenchymal stem cells (MSC) from horse fat tissue. The study used specific markers and observed their reactions. The cultivation technique proved effective and safe, and the researchers were able to characterize the isolated stem cells, suggesting potential use in horse cell therapy.

Isolation and Cultivation of MSC from Equine Adipose Tissue

  • Horse fat cells were collected from horses’ tail bases for the purpose of isolating and developing mesenchymal stem cells (MSC).
  • After the collection, the immediate isolation and cultivation of the MSC were undertaken.
  • The isolation and cell cultivation methods observed proved to be safe, yielding viable cells showing that the techniques used are not harmful and effectively produces the desired cells.

Use of Markers for Cellular Characterization

  • The markers used for cellular characterization were CD90, CD44, and CD13, which are common in stem cell research.
  • The tests conducted were an interspecies reaction test using mouse anti-rat CD90 monoclonal antibody (mAb), a fluorescent marker fluorescein isothiocyanate (FITC), and specific mAb tests with mouse anti-horse CD13 and mouse anti-horse CD44.
  • It was observed that CD90 mAb showed cross-reaction with equine adipose tissue-derived MSC. This implies that this marker reacts with different species’ MSC, validating its use in MSC research.
  • CD44 showed a higher expression in cells as the number of culture passages (cycles) increased. Every cycle allows the cells to multiply, which could have caused increased expression of this marker.
  • The CD13 marker didn’t show any expression contrary to other studies where CD13 often presents reactions involving MSC in different species. This suggests that equine MSC might have unique characteristics when compared to other species.

Potential Application and Conclusion

  • The study was able to successfully isolate, cultivate, and characterize MSC from horse adipose tissue.
  • Based on their findings, the research concludes that these particular stem cells have a promising future as progenitor cells (cells that create other types of cells) and could be applied in horse cell therapy.
  • This could potentially lead to developing new treatment methods for horses.

Cite This Article

APA
de Mattos Carvalho A, Alves AL, Golim MA, Moroz A, Hussni CA, de Oliveira PG, Deffune E. (2009). Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue. Vet Immunol Immunopathol, 132(2-4), 303-306. https://doi.org/10.1016/j.vetimm.2009.06.014

Publication

Veterinary immunology and immunopathology
ISSN: 1873-2534
NlmUniqueID: 8002006
Country: Netherlands
Language: English
Volume: 132
Issue: 2-4
Pages: 303-306

Researcher Affiliations

de Mattos Carvalho, Armando
  • Department of Veterinary Surgery and Anesthesiology, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, São Paulo, Brazil. armandomc@fmvz.unesp.br
Alves, Ana Liz Garcia
    Golim, Marjorie Assis
      Moroz, Andrei
        Hussni, Carlos Alberto
          de Oliveira, Patrícia Galvão Gomes
            Deffune, Elenice

              MeSH Terms

              • Adipose Tissue / cytology
              • Adipose Tissue / immunology
              • Animals
              • Antibodies, Monoclonal
              • CD13 Antigens / metabolism
              • Cell Separation
              • Flow Cytometry
              • Fluorescein-5-isothiocyanate
              • Fluorescent Dyes
              • Horses / anatomy & histology
              • Horses / immunology
              • Hyaluronan Receptors / metabolism
              • Immunophenotyping
              • Mesenchymal Stem Cells / classification
              • Mesenchymal Stem Cells / cytology
              • Mesenchymal Stem Cells / immunology
              • Mice
              • Rats
              • Thy-1 Antigens / metabolism

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