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Topic:Analytical Methods
Analytical methods in equine research encompass a variety of scientific techniques and tools used to study and evaluate different aspects of horse health, performance, and physiology. These methods help advance our understanding of equine biology, diagnosing conditions, and improving management practices. Common analytical methods include molecular techniques like PCR and ELISA for detecting pathogens and measuring biomarkers, imaging technologies such as ultrasound and MRI for assessing musculoskeletal health, and statistical models for analyzing genetic data and performance metrics. This page compiles peer-reviewed research studies and scholarly articles that explore the development, application, and impact of various analytical methods in equine science.
Determination of amphetamine in urine and blood. The determination of amphetamine in body fluids is of interest in veterinary toxicology because of the possible use of amphetamine in the doping of race horses. Many types of methods for its detection and determination have been developed. In the newest methods gas chromatography and mass spectrometry have been applied, making it possible to detect and identify 1 µg amphetamine in blood samples ( 1970).
Measurement of ligand-induced conformational changes in hemoglobin by circular dichroism. The UV circular-dichroism spectra of human and horse hemoglobins have been determined at various degrees of partial saturation with oxygen. Spectra of the two native hemoglobins were compared with spectra of the corresponding proteins modified with a reagent known to eliminate the conformational rearrangement normally associated with cooperativity. Such comparison indicates that one region, around 260 mmu, is sensitive chiefly to the state of the hemes; changes in another region, around 285 mmu, may be correlated with the conformational transformation linked to cooperative interactions. All ci...
A note on a simple estimation of amphetamine, methylamphetamine and ephedrine in horse urine. A chromatographic separation of amphetamine, methylamphetamine and ephedrine from horse urine is possible on alkaline Silica Gel G plates developed with acetone-methanol (1:3). After elution, the bases are determined colorimetrically. The intensity of the violet colour resulting from the nitration of amphetamine is measured in a Unicam SP1300 colorimeter using filter No. 1 (sensitivity 50–250 μg). The colour produced by the interaction of methylamphetamine, sodium nitro-prusside, acetaldehyde and triethanolamine is measured at 590 mμ (sensitivity 200–2,000 μg). Ephedrine was determined ...
Molecular weight distributions of milk fat triglycerides from seven species. The triglyceride compositions of the milk fats of man, dog, guinea pig, cow, sheep, goat, and horse were compared by gas-liquid chromatography of the intact triglycerides and of the butyl esters of the component fatty acids. The milk fats of man, dog, and guinea pig, which were largely made up of long-chain fatty acids, showed a common pattern with major contributions made by the glycerides with 48-54 acyl carbon atoms. The milk fats of cow, sheep, and goat, which were rich in short-chain acids, showed significant proportions of triglycerides with 28-54 acyl carbon atoms. Horse milk, which con...
Activation analysis of ungulate hair. Hair samples from the horse, elk, deer, moose, and antelope; subcutaneous tissue from the moose and antelope; and cast and living skin of the rattlesnake were activated by exposure to a neutron flux. The resulting products were studied by pulse-height analysis. Differences in type and proportion of trace elements appear to be consistent within the species studied.
