Analyze Diet

Topic:Animal Health

Animal Health encompasses a broad range of topics focused on maintaining and improving the well-being of equine species. This field addresses various aspects of horse care, including disease prevention, diagnosis, treatment, and management of health conditions. Key areas of interest include equine nutrition, vaccination protocols, parasite control, dental care, and the management of chronic conditions such as laminitis and colic. Additionally, animal health research in horses investigates the impact of exercise and training on physical health, the role of genetics in disease susceptibility, and the development of new therapeutic approaches. This page compiles peer-reviewed research studies and scholarly articles that explore the latest advancements, challenges, and best practices in preserving and enhancing the health of horses.
Effects of Differences in Fibre Composition and Maturity of Forage-Based Diets on the Microbial Ecosystem and Its Activity in Equine Caecum and Colon Digesta and Faeces.
Animals : an open access journal from MDPI    August 8, 2021   Volume 11, Issue 8 2337 doi: 10.3390/ani11082337
Muhonen S, Sadet-Bourgeteau S, Julliand V.Fibrous feeds are essential for horses. When developing feeding regimens promoting health and performance, we need to understand the digestion of plant cell walls and the functioning of the hindgut microbial ecosystem. Our objective was to investigate the effect of grass fibre maturity and legume forage on the hindgut microbiota and its activity. Six caecum and colon fistulated geldings were fed three diets differing in fibre composition: concentrate and late harvested grass haylage (35:65 energy ratio) (C); early and late harvested grass haylage (80:20) (G); lucerne and late harvested grass h...
Clinical insights: Recent advances in donkey medicine and welfare.
Equine veterinary journal    August 7, 2021   Volume 53, Issue 5 859-862 doi: 10.1111/evj.13471
Rickards K, Toribio RE.No abstract available
A Survey of Horse Selection, Longevity, and Retirement in Equine-Assisted Services in the United States.
Animals : an open access journal from MDPI    August 7, 2021   Volume 11, Issue 8 2333 doi: 10.3390/ani11082333
Rankins EM, Wickens CL, McKeever KH, Malinowski K.Little published information exists on the horses in equine-assisted services (EAS), particularly their selection, longevity, and retirement. The purpose of this study was to characterize horses and procedures used in EAS. A pilot survey was developed using focus group discussions and distributed to Professional Association of Therapeutic Horsemanship, International (PATH Intl) centers in Florida ( = 45, Part I) before further modification and distribution to members of PATH Intl., American Hippotherapy Association (AHA), eagala, and Certified Horsemanship Association (CHA) ( = 26,000, Part II...
Intestinal parasites in Przewalski’s horses (Equus ferus przewalskii): a field survey at the Hortobágy National Park, Hungary.
Journal of helminthology    August 6, 2021   Volume 95 e39 doi: 10.1017/S0022149X21000274
Jota Baptista C, Sós E, Szabados T, Kerekes V, Madeira de Carvalho L.The Pentezug Wildhorse Reserve, located in the Hortobágy National Park, Hungary, has one of the biggest ex situ populations of Przewalski's horses and aims to preserve its landscape and to study this subspecies. Between September and November 2018, 79 faecal samples were collected from Przewalski's horses. The McMaster, Willis flotation, natural sedimentation and coproculture methods were applied to all the samples. Results showed an average level of 1287 eggs per gram (EPG), which is a high faecal egg-shedding level. All the samples were positive for strongyle-type eggs (100%). There were no...
Steroidogenic Enzyme and Steroid Receptor Expression in the Equine Accessory Sex Glands.
Animals : an open access journal from MDPI    August 6, 2021   Volume 11, Issue 8 2322 doi: 10.3390/ani11082322
Ellerbrock RE, Podico G, Scoggin KE, Ball BA, Carossino M, Canisso IF.The expression pattern and distribution of sex steroid receptors and steroidogenic enzymes during development of the equine accessory sex glands has not previously been described. We hypothesized that equine steroidogenic enzyme and sex steroid receptor expression is dependent on reproductive status. Accessory sex glands were harvested from mature stallions, pre-pubertal colts, geldings, and fetuses. Expression of mRNA for estrogen receptor 1 (ESR1), estrogen receptor 2 (ESR2), androgen receptor (AR), 3β-Hydroxysteroid dehydrogenase/Δ5-4 isomerase (3βHSD), P450,17α hydroxylase, 17-20 lyase...
Comparative Trial of Continuous Flow Enteral and Intravenous Fluid Therapy in Horses.
Frontiers in veterinary science    August 6, 2021   Volume 8 686425 doi: 10.3389/fvets.2021.686425
Dias DCR, Ribeiro Filho JD, Viana RB, Bittencourt TCC, Dantas FTDR, Teixeira RBC, Di Filippo PA, Manso Filho HC, Alves SR, Santos PVM, Moreira NS.Continuous flow enteral fluid therapy with isotonic and hypotonic enteral electrolyte solutions are as safe and effective as intravenous fluid therapy. The aim of this study was to carry out a comparative assessment between continuous flow enteral and intravenous (IV) fluid therapy in adult experimentally dehydrated horses. Six experimentally dehydrated adult mares were used in a study carried out in a 6 × 3 crossover design, which each animal received three different treatments (isotonic enteral fluid therapy-EsISO, hypotonic enteral fluid therapy-EsHYPO and intravenous fluid therapy with La...
Collar pressure mapping: An evaluation of seven collar types used on working donkeys in Europe.
The Veterinary record    August 5, 2021   Volume 189, Issue 8 e772 doi: 10.1002/vetr.772
Rodrigues JB, Garrett C, Norris SL, Albuquerque F, Queijo L, Cooke F, Judge A.Working donkeys represent an important source of energy worldwide. Poor working conditions and equipment affect their ability to work. Poorly designed, ill-fitted harness causes inefficient transfer of power and leads to health and welfare issues. Using technology to assess different types of collars provides a better understanding of those that are most efficient for working donkeys. Methods: Seven different collars were tested using pressure pads. Contact area, median, maximum and peak pressures were obtained for the whole collar and critical points. Eight donkeys pulled 20% and 40% of their...
Horses show individual level lateralisation when inspecting an unfamiliar and unexpected stimulus.
PloS one    August 5, 2021   Volume 16, Issue 8 e0255688 doi: 10.1371/journal.pone.0255688
Animals must attend to a diverse array of stimuli in their environments. The emotional valence and salience of a stimulus can affect how this information is processed in the brain. Many species preferentially attend to negatively valent stimuli using the sensory organs on the left side of their body and hence the right hemisphere of their brain. Here, we investigated the lateralisation of visual attention to the rapid appearance of a stimulus (an inflated balloon) designed to induce an avoidance reaction and a negatively valent emotional state in 77 Italian saddle horses. Horses' eyes are late...
A critical evaluation for validation of composite and unidimensional postoperative pain scales in horses.
PloS one    August 5, 2021   Volume 16, Issue 8 e0255618 doi: 10.1371/journal.pone.0255618
Barreto da Rocha P, Driessen B, McDonnell SM, Hopster K, Zarucco L, Gozalo-Marcilla M, Hopster-Iversen C, Esteves Trindade PH, Gonzaga da Rocha TK....Proper pain therapy requires adequate pain assessment. This study evaluated the reliability and validity of the Unesp-Botucatu horse acute pain scale (UHAPS), the Orthopedic Composite Pain Scale (CPS) and unidimensional scales in horses admitted for orthopedic and soft tissue surgery. Forty-two horses were assessed and videotaped before surgery, up to 4 hours postoperatively, up to 3 hours after analgesic treatment, and 24 hours postoperatively (168 video clips). After six evaluators viewing each edited video clip twice in random order at a 20-day interval, they chose whether analgesia would b...
An investigation into environmental variables influencing post-race exertional heat illness in thoroughbred racehorses in temperate eastern Australia.
Australian veterinary journal    August 5, 2021   Volume 99, Issue 11 473-481 doi: 10.1111/avj.13108
Brownlow MA, Brotherhood JR.Exertional heat illness (EHI) is a syndrome that occurs when metabolic heat production from muscular contraction exceeds the rate at which it can be dissipated. Core body temperature rises to critical levels, causing hyperthermia and central nervous system dysfunction. Best practice for the prevention of EHI centres around early detection, rapid response and aggressive cooling. Advance planning enables risk mitigation measures. The more that is known about EHI in horses, the better prepared those in the positions of responsibility can be to anticipate events in which the risk of EHI may be ele...
Development and Validation of a Test for the Classification of Horses as Broken or Unbroken.
Animals : an open access journal from MDPI    August 4, 2021   Volume 11, Issue 8 2303 doi: 10.3390/ani11082303
Menchetti L, Dalla Costa E, Minero M, Padalino B.Regulation EC 1/2005 has stricter rules for transportation of unbroken (untamed) vs. broken (tamed) horses, but does not provide adequate tools for their identification. This study aimed to develop and validate such a tool. A behavioural test (Broken/Unbroken Test (BUT)) based on approaching, haltering, and leading was applied to 100 horses. Physiological and additional behavioural data were also collected, and the horses' status (broken/unbroken) was assessed by the expert who administered the BUT. Each horse's behaviour during the BUT was scored by four trained observers blinded to the horse...
Cryotherapy provides transient analgesia in an induced lameness model in horses.
The Canadian veterinary journal = La revue veterinaire canadienne    August 4, 2021   Volume 62, Issue 8 834-838 
Quam V, Yardley J, Quam M, Paz C, Belknap J.The analgesic effect of cryotherapy in an induced lameness model was evaluated. Lameness was induced with solar pressure from a custom-made shoe in a 10-horse, cross-over study. The degree of lameness was recorded with a commercial non-invasive inertial sensor. The distal limbs were maintained in an ice and water slurry (cryotherapy) or at ambient temperature (control) for 1 hour. Lameness was assessed serially over the following hour. Lameness at each time point was compared to the baseline induced lameness, within and between groups. Lameness had improved significantly in all horses 5 minute...
Active Circulation of Madariaga Virus, a Member of the Eastern Equine Encephalitis Virus Complex, in Northeast Brazil.
Pathogens (Basel, Switzerland)    August 3, 2021   Volume 10, Issue 8 983 doi: 10.3390/pathogens10080983
Gil LHVG, Magalhaes T, Santos BSAS, Oliveira LV, Oliveira-Filho EF, Cunha JLR, Fraiha ALS, Rocha BMM, Longo BC, Ecco R, Faria GC, Furtini R....Madariaga virus (MADV) is a member of the eastern equine encephalitis virus (EEEV) complex that circulates in Central and South America. It is a zoonotic, mosquito-borne pathogen, belonging to the family . Disturbances in the natural transmission cycle of this virus result in outbreaks in equines and humans, leading to high case fatality in the former and acute febrile illness or neurological disease in the latter. Although a considerable amount of knowledge exists on the eco-epidemiology of North American EEEV strains, little is known about MADV. In Brazil, the most recent isolations of MADV ...
Some Genetic and Environmental Effects on Equine Asthma in Polish Konik Horses.
Animals : an open access journal from MDPI    August 3, 2021   Volume 11, Issue 8 2285 doi: 10.3390/ani11082285
Borowska A, Wolska D, Niedzwiedz A, Borowicz H, Jaworski Z, Siemieniuch M, Szwaczkowski T.Current knowledge of the genetic and environmental backgrounds of equine asthma seems to be insufficient, especially for primitive horse breeds. The main objectives of this study were to estimate the effects of sex, birth period, stud, parentage line and inbreeding on asthma morbidity in Polish Konik horses. Records of 274 horses (housed in two studs) were analysed. These animals were allocated to maternal and paternal lines. Individual inbreeding coefficients were extracted from the additive relationship matrix. Horses underwent diagnosis based on observation of the basic symptoms (high frequ...
The Role of Mesenchymal Stem Cells (MSCs) in Veterinary Medicine and Their Use in Musculoskeletal Disorders.
Biomolecules    August 2, 2021   Volume 11, Issue 8 doi: 10.3390/biom11081141
Prządka P, Buczak K, Frejlich E, Gąsior L, Suliga K, Kiełbowicz Z.Regenerative medicine is a dynamically developing field of human and veterinary medicine. The animal model was most commonly used for mesenchymal stem cells (MSCs) treatment in experimental and preclinical studies with a satisfactory therapeutic effect. Year by year, the need for alternative treatments in veterinary medicine is increasing, and other applications for promising MSCs and their biological derivatives are constantly being sought. There is also an increase in demand for other methods of treating disease states, of which the classical treatment methods did not bring the desired resul...
Analysis of volatiles from feces of released Przewalski’s horse (Equus przewalskii) in Gasterophilus pecorum (Diptera: Gasterophilidae) spawning habitat.
Scientific reports    August 2, 2021   Volume 11, Issue 1 15671 doi: 10.1038/s41598-021-95162-9
Zhou R, Yang J, Zhang K, Qi Y, Ma W, Wang Z, Ente M, Li K.The absolute dominant species that infests wild population of Przewalski's horse (Equus przewalskii) is Gasterophilus pecorum, and feces of released Przewalski's horse, a habitat odor, plays an important role in mating and ovipositing locations of G. pecorum. To screen out unique volatiles for attracting G. pecorum, volatiles from fresh feces of released horses at stages of pre-oviposition (PREO), oviposition (OVIP), and post-oviposition (POSO) of G. pecorum, and feces with three different freshness states (i.e., Fresh, Semi-fresh, and Dry) at OVIP were collected by dynamic headspace adsorptio...
Factors Associated with Owner-Reported Euthanasia in Equids in France.
Journal of equine veterinary science    July 31, 2021   Volume 105 103723 doi: 10.1016/j.jevs.2021.103723
Merlin A, Foucher N, Linster M, Cazeau G, Marsot M, Delerue M, Sala C, Schneider J, Ferry B, Amat JP, Tapprest J.There is limited evidence regarding the proportion of dead equids in France that were euthanized and the factors influencing the decision-making of euthanasia. The better understanding of which could facilitate research on improvement of welfare, especially on end of life issues. The aim of this study was to estimate the proportion of euthanasia and identify associated factors in equids in France. A web-based survey was created and distributed by the French horse and riding institute to owners who reported an equine death between April 2017 and April 2018 (n = 5 158). Factors associated with...
Detecting Welfare in a Non-Verbal Species: Social/Cultural Biases and Difficulties in Horse Welfare Assessment.
Animals : an open access journal from MDPI    July 30, 2021   Volume 11, Issue 8 2249 doi: 10.3390/ani11082249
Hausberger M, Lesimple C, Henry S.Horses were domesticated for more than 5000 years and have been one of the most emblematic species living alongside humans. This long-shared history would suggest that horses are well known and well understood, but scientific data raise many concerns about the welfare state of most domestic horses suggesting that many aspects have been largely misunderstood. In the present review, we will examine some of the possible human factors that may explain the huge prevalence of welfare problems, despite horses being of special importance to humans. First of all, as horses are non-verbal, current manag...
Serological Evidence of Common Equine Viral Infections in a Semi-Isolated, Unvaccinated Population of Hucul Horses.
Animals : an open access journal from MDPI    July 30, 2021   Volume 11, Issue 8 2261 doi: 10.3390/ani11082261
Bażanów B, Pawęska JT, Pogorzelska A, Florek M, Frącka A, Gębarowski T, Chwirot W, Stygar D.Huculs () are an old breed of primitive mountain horses, originating from the Carpathian Mountains. To the best of our knowledge, data concerning the epidemiology of viral infections observed within this breed are sparse. The objective of this study was to estimate the serological status of a semi-isolated, unvaccinated Hucul herd, with respect to both common equine viral infections and horse-infecting arboviruses, the presence of which was previously reported in Poland. Twenty horses of the Hucul breed, living in a remote area in Poland, were studied in 2018 from March to May. Using nasal sec...
Sequence analysis of the mitochondrial D-loop region throws a new light on the origin of Hungarian Nonius, Danubian Horse and Serbian Nonius.
Acta veterinaria Hungarica    July 30, 2021   Volume 69, Issue 3 239-248 doi: 10.1556/004.2021.00029
Yordanov G, Zlatanovic N, Palova N, Mehandjyiski I, Neov B, Radoslavov G, Hristov P.The objective of our study was to investigate the genetic structure of yet uninvestigated populations of three closely related horse breeds - the Danubian Horse, the Hungarian Nonius and the Serbian Nonius - in order to clarify their origin and genetic diversity. A 640-bp-long fragment of the mtDNA D-loop region was amplified and sequenced. The results showed that the investigated breeds have different genetic profiles although they share some common characteristics. We identified nine of the 17 haplogroups described in modern horses. Most of the obtained sequences fall into the M, L, G, and O...
Investigations of the maintenance system of the Konik Polski horse and its effects on fecal microbiota activity during the winter and summer seasons.
Animal science journal = Nihon chikusan Gakkaiho    July 29, 2021   Volume 92, Issue 1 e13603 doi: 10.1111/asj.13603
Juśkiewicz J, Fotschki B, Jaworska J, Siemieniuch M.Gastrointestinal microbiota play a key role in the nutrients digestion and hence maintaining animal health and welfare. The diet offered to the animals in captivity may differ considerably from that on natural pastures. In a stabled maintenance system, horses have a limited choice of habitat and feed. Time spend for feeding is relevant for equine welfare because the reduction of the time devoted for foraging may be responsible for inducing gastric inflammation and ulceration. Therefore, in the present study, it was hypothesized that fecal bacterial fermentative processes differ between free-ro...
Assessment of qualitative and quantitative parameters of healthy thyroid gland in adult horses by magnetic resonance imaging.
Anatomia, histologia, embryologia    July 28, 2021   Volume 50, Issue 5 839-848 doi: 10.1111/ahe.12726
Viana GF, Midon M, Fonseca MW, Hataka A, Carandina L, Bueno LMC, Puoli Filho JNP, Machado VMV.In humans and pets, magnetic resonance imaging (MRI) provides important adjunctive anatomic information about the thyroid and its adjacent structures. To the best of our knowledge, however, no studies have been reported regarding the use of MRI for thyroid evaluation in horses. The aim of this study was to obtain qualitative and quantitative parameters of healthy thyroid gland in adult horses by MRI. Eleven horses were submitted to thyroid MRI evaluation. The healthy thyroid of these animals was ensured by normal examinations performed before (free thyroxine and ultrasound) and after (cytology...
Safety of a feed additive consisting of a tincture derived from Verbascum thapsus L. (great mullein tincture) for use in all animal species (MANGHEBATI SAS).
EFSA journal. European Food Safety Authority    July 28, 2021   Volume 19, Issue 7 e06711 doi: 10.2903/j.efsa.2021.6711
Bampidis V, Azimonti G, Bastos ML, Christensen H, Fašmon Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....The tincture derived from L. (great mullein tincture) is intended to be used as a sensory additive in feed for all animal species. The product is a water/ethanol solution, with a dry matter content of ˜ 2.8% and contains on average 0.216% polyphenols including 0.093% flavonoids. According to a previous assessment, the additive was not characterised in full and about 82% of the dry matter fraction remained uncharacterised (representing 2.26% of the tincture). There was also uncertainty on the potential presence of iridoid glycosides in the tincture. Therefore, the Panel on Additives and Prod...
Impact of blue light therapy on plasma adrenocorticotropic hormone (ACTH) and hypertrichosis in horses with pituitary pars intermedia dysfunction.
Domestic animal endocrinology    July 27, 2021   Volume 78 106651 doi: 10.1016/j.domaniend.2021.106651
Miller AB, Murphy BA, Adams AA.Blue light therapy can be used in horses to alter the natural photoperiod and inhibit winter hair coat growth. Seasonal increases in ACTH occur in the fall season but are exaggerated in horses with pituitary pars intermedia dysfunction (PPID). Additionally, PPID horses frequently present with hypertrichosis. Thus, blue light therapy was proposed as a potential management tool for hypertrichosis and for investigating the impact of photoperiod manipulation on ACTH. Eighteen PPID horses, aged 18 to 31 yr, from a university-owned research herd were selected and assigned to either the control group...
A comprehensive review on avian chlamydiosis: a neglected zoonotic disease.
Tropical animal health and production    July 27, 2021   Volume 53, Issue 4 414 doi: 10.1007/s11250-021-02859-0
Ravichandran K, Anbazhagan S, Karthik K, Angappan M, Dhayananth B.Avian chlamydiosis is one of the important neglected diseases with critical zoonotic potential. Chlamydia psittaci, the causative agent, affects most categories of birds, livestock, companion animals, and humans. It has many obscured characters and epidemiological dimensions, which makes it unique among other bacterial agents. Recent reports on transmission from equine to humans alarmed the public health authorities, and it necessitates the importance of routine screening of this infectious disease. High prevalence of spill-over infection in equines was associated with reproductive losses. New...
Total Intravenous Anaesthesia with Ketamine, Medetomidine and Midazolam as Part of a Balanced Anaesthesia Technique in Horses Undergoing Castration.
Veterinary sciences    July 26, 2021   Volume 8, Issue 8 142 doi: 10.3390/vetsci8080142
Cunneen A, Pratt S, Perkins N, McEwen M, Truchetti G, Rainger J, Farry T, Kidd L, Goodwin W.To evaluate the use of ketamine-medetomidine-midazolam total intravenous infusion as part of a balanced anaesthetic technique for surgical castration in horses. Five healthy Standardbred cross colts were premedicated with IV acepromazine (0.01-0.02 mg/kg), medetomidine (7 µg/kg) and methadone (0.1 mg/kg) and anaesthesia induced with IV ketamine (2.2 mg/kg) and midazolam (0.06 mg/kg). Horses were anaesthetised for 40 min with an IV infusion of ketamine (3 mg/kg/h), medetomidine (5 µg/kg/h) and midazolam (0.1 mg/kg/h) while routine surgical castration was performed. Cardiorespiratory variables...
Associative Effects between Forages and Concentrates on In Vitro Fermentation of Working Equine Diets.
Animals : an open access journal from MDPI    July 26, 2021   Volume 11, Issue 8 2212 doi: 10.3390/ani11082212
Gandarillas M, Keim JP, Gapp EM.Horses are hindgut fermenters, and it is therefore important to determine the postgastric nutritive value of their feedstuffs and diets. Moreover, it has been demonstrated in other animal species that the fermentation of diets results in different values than those expected from pure ingredients. Therefore, the general objective of this work is to evaluate the gas production (GP) and volatile fatty acid (VFA) concentration, as well as the associative effects, of mixtures of different forages and concentrated foods, which are representative of the traditional diets of high-performance horses. M...
Spread of anthelmintic resistance in intestinal helminths of dogs and cats is currently less pronounced than in ruminants and horses – Yet it is of major concern.
International journal for parasitology. Drugs and drug resistance    July 25, 2021   Volume 17 36-45 doi: 10.1016/j.ijpddr.2021.07.003
von Samson-Himmelstjerna G, Thompson RA, Krücken J, Grant W, Bowman DD, Schnyder M, Deplazes P.Anthelmintic resistance (AR) has thus far only rarely been reported for intestinal helminths of dogs and cats, in contrast to parasites of livestock and horses. We highlight possible reasons for this striking and important discrepancy, including ecological, biological and genetic factors and/or intervention regimens of key intestinal helminths concerning both host groups. In view of the current knowledge related to the genetics, mechanisms and principles of AR development, we point at issues which in our view contribute to a comparatively lower risk of AR development in intestinal helminths of...
Hair Cortisol, Testosterone, Dehydroepiandrosterone Sulfate and Their Ratios in Stallions as a Retrospective Measure of Hypothalamic-Pituitary-Adrenal and Hypothalamic-Pituitary-Gonadal Axes Activity: Exploring the Influence of Seasonality.
Animals : an open access journal from MDPI    July 25, 2021   Volume 11, Issue 8 2202 doi: 10.3390/ani11082202
Olvera-Maneu S, Carbajal A, Gardela J, Lopez-Bejar M.The monitoring of stress physiology includes studying a wide range of endocrinological mechanisms, which can be assessed using multiple tissue samples. This study aimed to evaluate the seasonal variations of hair C, T and DHEA-S in horses for a whole year, as well as to assess the variations between seasons of C/DHEA-S and T/C ratios as a retrospective measure of the hypothalamic-pituitary-adrenal and hypothalamic-pituitary-gonadal axis activity. Ten pure-breed Menorca stallions were included in the study. The hair samples were collected approximately every two months following the shave-resha...
Identical Viral Genetic Sequence Found in Black Flies (Simulium bivittatum) and the Equine Index Case of the 2006 U.S. Vesicular Stomatitis Outbreak.
Pathogens (Basel, Switzerland)    July 23, 2021   Volume 10, Issue 8 doi: 10.3390/pathogens10080929
Drolet BS, Reeves WK, Bennett KE, Pauszek SJ, Bertram MR, Rodriguez LL.In 2006, vesicular stomatitis New Jersey virus (VSNJV) caused outbreaks in Wyoming (WY) horses and cattle after overwintering in 2004 and 2005. Within two weeks of the outbreak onset, 12,203 biting flies and 194 grasshoppers were collected near three equine-positive premises in Natrona County, WY. Insects were identified to the species level and tested by RT-qPCR for VSNJV polymerase (L) and phosphoprotein (P) gene RNA. Collected dipterans known to be competent for VSV transmission included Simulium black flies and Culicoides biting midges. VSNJV L and P RNA was detected in two pools of female...