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Topic:Diagnostic Technique

Diagnostic techniques in equine medicine encompass a range of procedures and tools used to identify diseases, injuries, or other health conditions in horses. These techniques include imaging methods such as radiography, ultrasonography, and magnetic resonance imaging (MRI), as well as laboratory tests like blood work and tissue biopsies. Each diagnostic method provides specific information that can aid in the assessment and management of equine health issues. Radiography is commonly used for evaluating bone structures, while ultrasonography is useful for soft tissue examination. MRI offers detailed images of both soft and hard tissues, though its use is limited by cost and availability. Laboratory tests can detect biochemical and hematological changes indicative of disease. This page compiles peer-reviewed research studies and scholarly articles that explore the development, application, and efficacy of various diagnostic techniques in equine veterinary practice.
Ileal impaction in 245 horses: 1995-2007.
The Canadian veterinary journal = La revue veterinaire canadienne    January 3, 2012   Volume 52, Issue 7 759-763 
Fleming K, Mueller PO.The objective of this study was to identify parameters that would assist in determining the probability of a successful outcome with medical management versus surgical intervention in horses with ileal impaction. Medical records of 245 horses admitted for ileal impaction were reviewed and placed into 2 groups: medical (med) and surgical (sx) treatment. Persistence of abdominal pain, gastric reflux, frequency of analgesic administration, and 1-year survival were evaluated. There were no differences in signalment, abdominal pain, or heart rate among groups; however, significantly more sx horses ...
Equine laparoscopy: abdominal access techniques.
Compendium (Yardley, PA)    January 1, 2012   Volume 34, Issue 11 E2 
Caron JP.No abstract available
Case report: squamous cell carcinoma of the urinary bladder in a horse.
Compendium (Yardley, PA)    January 1, 2012   Volume 34, Issue 10 E1-E5 
Zantingh AJ, Gaughan EM, Bain FT.A 14-year-old Hanoverian gelding was evaluated after a 10-week history of occasional straining during urination and dark red urine production after exercise. Physical examination findings were within normal limits. After 2 weeks of clinical signs, ultrasonography had found no abnormalities in the kidneys or bladder. A freely voided urine sample revealed an elevated protein concentration (500 mg/dL; reference range: <100 mg/dL) and few red blood cells. Vitamin C supplementation was suggested to acidify the urine, as was provision of a salt block to encourage water intake.
Prevalence and intensity of gastro-intestinal helminths in horses in the Sudano-Guinean climatic zone of Cameroon.
Tropical parasitology    January 1, 2012   Volume 2, Issue 1 45-48 doi: 10.4103/2229-5070.97239
Lem MF, Vincent KP, Pone JW, Joseph T.It is important to understand the biology and health impact of parasites affecting horses in the Western highlands of Cameroon. Objective: to evaluate the prevalence and intensity of infection of gastrointestinal helminths in these animals. Methods: A total of 894 horses (367 males and 527 females), and aged ≤1 to ≥7 years old were examined. A parasitological investigation of faeces was carried out using two techniques: concentration method of Full Born Willis (flotation in saturated solution of sodium chloride) and Mc Master Technique. Conclusions: From the results the overall prevalence ...
Examination of the depth of the equine hard palate.
Journal of veterinary dentistry    January 1, 2012   Volume 29, Issue 4 228-230 doi: 10.1177/089875641202900403
Evans RG, Lowder M.Equine behavioral problems and loss of optimum performance have been associated with musculoskeletal injury and, more recently, dental disease. Injuries to a horse's tongue and bars of the mandible due to bitting have been documented. However, another point of contact of the bit, the hard palate, has thus far been virtually ignored. The objective of the study was to determine if there was a significant range associated with the depth of the equine hard palate and if this range was associated with a certain breed, age, or sex of the horse. Oral examinations were performed on 52 horses and a mea...
Recombinant envelope protein (rgp90) ELISA for equine infectious anemia virus provides comparable results to the agar gel immunodiffusion.
Journal of virological methods    December 30, 2011   Volume 180, Issue 1-2 62-67 doi: 10.1016/j.jviromet.2011.12.012
Reis JK, Diniz RS, Haddad JP, Ferraz IB, Carvalho AF, Kroon EG, Ferreira PC, Leite RC.Equine infectious anemia (EIA) is an important viral infection affecting horses worldwide. The course of infection is accompanied generally by three characteristic stages: acute, chronic and inapparent. There is no effective EIA vaccine or treatment, and the control of the disease is based currently on identification of EIAV inapparent carriers by laboratory tests. Recombinant envelope protein (rgp90) was expressed in Escherichia coli and evaluated via enzyme-linked immunosorbent assay (ELISA). There was an excellent agreement (95.42%) between the ELISA results using rgp90 and agar gel immunod...
Scintigraphic evaluation of intra-arterial and intravenous regional limb perfusion of allogeneic bone marrow-derived mesenchymal stem cells in the normal equine distal limb using (99m) Tc-HMPAO.
Equine veterinary journal    December 30, 2011   Volume 44, Issue 5 594-599 doi: 10.1111/j.2042-3306.2011.00530.x
Sole A, Spriet M, Galuppo LD, Padgett KA, Borjesson DL, Wisner ER, Brosnan RJ, Vidal MA.Mesenchymal stem cells (MSCs) are commonly injected intralesionally for treatment of soft tissue injuries in the horse. Alternative routes of administration would be beneficial for treatment of lesions that cannot be accessed directly or to limit needle-induced iatrogenic damage to the surrounding tissue. Objective: The purpose of our study was to evaluate MSC distribution after intra-arterial (IA) and intravenous (IV) regional limb perfusions (RLP) using scintigraphy. We hypothesised that MSCs would persist in the distal limb after tourniquet removal and that both techniques would lead to dif...
Effects of tidal volume, ventilatory frequency, and oxygen insufflation flow on the fraction of inspired oxygen in cadaveric horse heads attached to a lung model.
American journal of veterinary research    December 30, 2011   Volume 73, Issue 1 134-139 doi: 10.2460/ajvr.73.1.134
Crumley MN, Hodgson DS, Kreider SE.To measure the effects of tidal volume, ventilatory frequency, and oxygen insufflation flow on the fraction of inspired oxygen in cadaveric horse heads attached to a lung model. Methods: 8 heads of equine cadavers. Methods: Each cadaveric horse head was intubated with a nasotracheal tube that extended into the proximal portion of the trachea. Oxygen was delivered through an oxygen catheter contained within and extending to the tip of the nasotracheal tube. The trachea was connected to the lung model by use of a spiral-wound hose with a sampling adaptor. Eight treatment combinations involving 2...
p- and m-Cresols emitted from estrous urine are reliable volatile chemical markers of ovulation in mares.
Animal reproduction science    December 29, 2011   Volume 130, Issue 1-2 51-56 doi: 10.1016/j.anireprosci.2011.12.008
Mozūraitis R, Būda V, Kutra J, Borg-Karlson AK.Urine samples from 14 mares, belonging to five breeds, were collected at estrus and diestrus to search for estrous specific volatile compounds which could be used to determine ovulation time. Around 150 volatiles were collected from urine head-space samples by solid phase micro extraction technique, and analyses were conducted by gas chromatography-mass spectrometry methods. Comparison of chromatographic profiles of volatile substances revealed that concentrations of m- and p-cresols were significantly greater during estrus while diethylphthalate was more abundant at diestrus. Monitoring of m-...
Development and laboratory evaluation of two lateral flow devices for the detection of vesicular stomatitis virus in clinical samples.
Journal of virological methods    December 29, 2011   Volume 180, Issue 1-2 96-100 doi: 10.1016/j.jviromet.2011.12.010
Ferris NP, Clavijo A, Yang M, Velazquez-Salinas L, Nordengrahn A, Hutchings GH, Kristersson T, Merza M.Two lateral flow devices (LFD) for the detection of vesicular stomatitis (VS) virus (VSV), types Indiana (VSV-IND) and New Jersey (VSV-NJ) were developed using monoclonal antibodies C1 and F25VSVNJ-45 to the respective VSV serotypes. The performance of the LFDs was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of VSV. The collection of test samples included 105 positive for VSV-IND (92 vesicular epithelial suspensions and 13 cell culture antigens; encompassing 93 samples of subtype 1 [VSV-IND-1], 9 of subtype 2 [VSV-IND-2] and 3...
Use of furosemide for exercise-induced pulmonary hemorrhage in racehorses.
Journal of the American Veterinary Medical Association    December 27, 2011   Volume 239, Issue 11 1407 
Hinchcliff KW, Morley PS, Guthrie A.No abstract available
Latent equine herpesvirus-1 in trigeminal ganglia and equine idiopathic headshaking.
Journal of veterinary internal medicine    December 23, 2011   Volume 26, Issue 1 192-194 doi: 10.1111/j.1939-1676.2011.00855.x
Aleman M, Pickles KJ, Simonek G, Madigan JE.Trigeminal neuralgia or neuropathic pain has been regarded as a putative cause of idiopathic headshaking in horses. Equine herpesvirus-1 (EHV-1) infection and resultant postherpetic pain have been suggested as a possible cause of such neuropathic pain. Objective: To determine the presence of EHV-1 in the trigeminal ganglia of horses with idiopathic headshaking. Methods: Nineteen horses: control (n = 11, 9 geldings, 2 mares, median age 11 years) and headshaking (n = 8, all geldings, median age 11.5 years) horses were sourced from the equine research herd and caseload at the Veterinary Medical T...
Association between circulating angiotensin-converting enzyme and exercise-induced pulmonary haemorrhage in Thoroughbred racehorses.
Research in veterinary science    December 22, 2011   Volume 93, Issue 2 993-994 doi: 10.1016/j.rvsc.2011.10.011
Costa MF, Ronchi FA, Ivanow A, Carmona AK, Casarini D, Slocombe RF.Exercise-induced pulmonary haemorrhage has an impact on racehorse performance. Although endoscopic diagnosis (with or without the aid of bronchoalveolar lavage) is considered to be the standard diagnostic method for this condition, the use of biomarkers that could aid in quantifying risk and severity of the condition would represent an advance in equine sport medicine. This preliminary research investigated the use of angiotensin-converting enzyme (ACE) activity in plasma of racehorses and demonstrated that ACE activity is increased in horses with higher degrees of haemorrhage and is a promisi...
Three-dimensional ultrasound imaging of the equine fetus.
Theriogenology    December 20, 2011   Volume 77, Issue 7 1480-1486 doi: 10.1016/j.theriogenology.2011.10.020
Kotoyori Y, Yokoo N, Ito K, Murase H, Sato F, Korosue K, Nambo Y.The objective was to assess the optimal procedure for real-time, three-dimensional (3D) ultrasound (US) imaging for assessing the equine fetus during the first half of gestation and the possibility of using 3D US imaging of the equine fetus in clinical applications. Seventeen pregnant mares were examined by 3D US between Days 35 and 180 of gestation. Abdominal and endo-vaginal real-time 3D transducers used in human medicine were used for transrectal and transvaginal examinations, respectively. Images were recorded by both 3D stationary and real-time movies. In a comparison of four methods, tra...
Lactate production and measurement in critically ill horses.
Compendium (Yardley, PA)    December 20, 2011   Volume 33, Issue 12 E5 
Tennent-Brown BS.Blood lactate concentration can be easily measured by practitioners using inexpensive point-of-care meters. Anaerobic tissue metabolism resulting from inadequate oxygen delivery (DO2) is the most important cause of an increase in blood lactate concentration in equine patients. However,hyperlactatemia also occurs under conditions of apparently adequate DO2, usually in association with sepsis and an intense inflammatory reaction. Numerous mechanisms have been proposed for aerobic hyperlactatemia, including increased Na+/K+-ATPase activity in response to inflammatory mediators; inhibition of pyru...
Conservative management of a transverse fracture of the distal phalanx in a Quarter Horse.
Journal of the American Veterinary Medical Association    December 17, 2011   Volume 240, Issue 1 82-86 doi: 10.2460/javma.240.1.82
Sherlock CE, Eggleston RB, Howerth EW.A 7-year-old Quarter Horse gelding was evaluated because of sudden onset of severe left forelimb lameness of 4 days' duration. Results: Clinical evaluation and diagnostic perineural analgesia localized the lameness to the distal portion of the left forelimb. Radiography revealed a transverse fracture of the distal phalanx of the left forelimb. Results: The horse was treated conservatively with stall rest and stabilization of the hoof with fiberglass cast material and an elevated heel support. These treatments improved the lameness considerably. Over the following 4 months, the horse was exerci...
Nonruminant Nutrition Symposium: Involvement of gut neural and endocrine systems in pathological disorders of the digestive tract.
Journal of animal science    December 16, 2011   Volume 90, Issue 4 1203-1212 doi: 10.2527/jas.2011-4825
Furness JB, Poole DP.The functioning of the gastrointestinal tract is under the control of the most extensive system of peripheral neurons in the body, the enteric nervous system, and the largest endocrine system of the body, the GEP endocrine system. The enteric nervous system in large mammals contains 500 million neurons, and the GEP endocrine system produces more than 30 hormones. Numerous enteric neuropathies affecting both humans and animals have been described and digestive disorders affect commercially important species, such as horses and cattle. The most severe enteric neuropathies (e.g., lethal white syn...
Reference ranges and reliability of transabdominal ultrasonographic renal dimensions in thoroughbred horses. Draper AC, Bowen IM, Hallowell GD.The aims of this study were to establish a normal reference range (mean ± 2 SD) and assess reliability of renal dimensions obtained using transabdominal ultrasonography in Thoroughbred horses (n = 7). A minimum of three ultrasonographic cineloops were obtained from each intercostal space and the left paralumbar fossa by two observers daily for three consecutive days. Renal length, width, and thickness and cortex, medulla, and pelvic dimensions were obtained. Measurements were undertaken by both observers, who were unaware of prior measurements, to assess reproducibility and measured on three ...
Comparison of eosin-thiazin and Papanicolaou-Shorr staining for endometrial cytologies of broodmares. Technical Note.
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    December 15, 2011   Volume 39, Issue 6 358-362 
Walter J, Klein C, Wehrend A.Exfoliative endometrial cytology is an easy and valuable diagnostic tool for the detection of inflammatory processes of the uterus that correlates well to culture results. The practical use of this procedure is limited due to the time-consuming Papanicolaou-Shorr staining technique. In this study the suitability of the rapid eosin-thiazin staining for endometrial smears was investigated. Methods: Sample collection was carried out with a guarded culture swab (Knudsen-catheter) in 27 broodmares during routine gynaecological examination. Two smears were prepared from each collection. One was stai...
Epilepsy in horses: aetiological classification and predictive factors.
Equine veterinary journal    December 14, 2011   Volume 44, Issue 6 646-651 doi: 10.1111/j.2042-3306.2011.00527.x
Lacombe VA, Mayes M, Mosseri S, Reed SM, Fenner WR, Ou HT.In contrast with reports in man and small animals, a systematic classification of seizures in horses is lacking. Objective: The purpose of this study was to classify seizures based on their aetiology and to characterise epilepsy in 104 horses presented for seizures at the Ohio State University Veterinary Medical Center between 1988 and 2009. Methods: In a retrospective observational study, seizures were classified by aetiology based on history, clinical observations, diagnostic investigations (e.g. electroencephalograms, cerebrospinal fluid and computed tomography imaging of the head) and post...
Oesophageal electrocardiography in healthy horses.
Equine veterinary journal    December 14, 2011   Volume 44, Issue 6 640-645 doi: 10.1111/j.2042-3306.2011.00526.x
Verheyen T, Decloedt A, De Clercq D, Sys S, Van Loon G.In human medicine, oesophageal electrocardiography (ECG) is a well-established technique that magnifies P waves with respect to the QRS complex. Objective: To investigate the feasibility of oesophageal ECG recording in horses and its ability to produce larger P waves compared with base-apex and unipolar recordings. Methods: Bipolar and unipolar ECG were performed using oesophageal and surface electrodes. Oesophageal ECG was obtained from 6 different recording configurations at different oesophageal depths. Amplitudes of P, Q, R, S and T waves were measured from 3 different cardiac cycles for e...
Stifle extension results in differential tensile forces developing between abaxial and axial components of the cranial meniscotibial ligament of the equine medial meniscus: a mechanistic explanation for meniscal tear patterns.
Equine veterinary journal    December 14, 2011   Volume 44, Issue 5 554-558 doi: 10.1111/j.2042-3306.2011.00532.x
Fowlie JG, Arnoczky SP, Lavagnino M, Stick JA.To identify potential functional-anatomical characteristics of the cranial horn attachment of the medial meniscus (MM) that may help explain the pathogenesis of the common tear patterns that have been reported. Objective: Full extension of the stifle generates a significant increase in tensile forces within the cranial meniscotibial ligament (CrMTL) of the MM, which may predispose this structure to injury. Methods: The effect of femorotibial angle (160°, 150°, 140° and 130°) on tensile forces in the axial and abaxial components of the CrMTL was examined in 6 mature cadaver stifles using an...
Complex dynamic upper airway collapse: associations between abnormalities in 99 harness racehorses with one or more dynamic disorders.
Equine veterinary journal    December 13, 2011   Volume 44, Issue 5 524-528 doi: 10.1111/j.2042-3306.2011.00516.x
Strand E, Skjerve E.Many horses demonstrate dynamic collapse of more than one upper respiratory tract (URT) structure during high-speed treadmill videoendoscopy (HSTV). Objective: To report the frequency of complex dynamic URT collapse in harness racehorses and determine if an association exists between occurrence of certain disorders. Methods: Retrospective study of 99 Standardbreds and Coldblooded trotters that had one or more dynamic URT disorders identified during HSTV between 1998 and 2006. The horses underwent HSTV using a protocol that included periods of free head carriage and poll flexion until fully fat...
Performance characteristics of a conformal ultra-wideband multilayer applicator (CUMLA) for hyperthermia in veterinary patients: a pilot evaluation of its use in the adjuvant treatment of non-resectable tumours.
Veterinary and comparative oncology    December 13, 2011   Volume 11, Issue 1 14-29 doi: 10.1111/j.1476-5829.2011.00297.x
Smrkovski OA, Koo Y, Kazemi R, Lembcke LM, Fathy A, Liu Q, Phillips JC.Performance and clinical characteristics of a novel hyperthermia antenna operating at 434 MHz were evaluated for the adjuvant treatment of locally advanced superficial tumours in cats, dogs and horses. Electromagnetic simulations were performed to determine electric field characteristics and compared to simulations for a flat microwave antenna with similar dimensions. Simulation results show a reduced skin surface and backfield irradiation and improved directional irradiation (at broadside) compared to a flat antenna. Radiated power and penetration is notably increased with a penetration depth...
Influence of counting chamber type on CASA outcomes of equine semen analysis.
Equine veterinary journal    December 12, 2011   Volume 44, Issue 5 542-549 doi: 10.1111/j.2042-3306.2011.00523.x
Hoogewijs MK, de Vliegher SP, Govaere JL, de Schauwer C, de Kruif A, van Soom A.Sperm motility is considered to be one of the key features of semen analysis. Assessment of motility is frequently performed using computer-assisted sperm analysis (CASA). Nevertheless, no uniform standards are present to analyse a semen sample using CASA. Objective: We hypothesised that the type of counting chamber used might influence the results of analysis and aimed to study the effect of chamber type on estimated concentration and motility of an equine semen sample assessed using CASA. Methods: Commonly used disposable Leja chambers of different depths were compared with disposable and re...
Cardiac biomarkers in equine medicine.
Veterinary journal (London, England : 1997)    December 7, 2011   Volume 192, Issue 2 131-132 doi: 10.1016/j.tvjl.2011.11.011
Jesty SA.No abstract available
In vitro comparison of three suture techniques for anastomosis of the equine small intestine.
Equine veterinary journal. Supplement    December 7, 2011   Issue 40 46-50 doi: 10.1111/j.2042-3306.2011.00494.x
Auletta L, Lamagna F, Uccello V, Lamagna B, Pasolini MP.The equine small intestine can be affected by a variety of disorders that may require some form of bypass or anastomosis. Many suture patterns have been used in equine anastomoses to minimise post operative complications, which include leakage from the anastomosis site, stenosis and adhesions. Because of the critical condition of horses undergoing colic surgery, it is imperative this is performed as quickly as possible. Objective: To evaluate, in vitro, differences in the time of execution and leakage pressure between the Lembert single layer, Gambee and Lembert double layer suture patterns fo...
Comparison of three methods of sampling for endometrial cytology in the mare. Preliminary study.
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    December 6, 2011   Volume 39, Issue 3 171-175 
Defontis M, Vaillancourt D, Grand FX.This prospective study aims to compare three different sampling techniques for the collection of endometrial cytological specimens in the mare: the guarded culture swab, the uterine cytobrush and the low volume uterine flush. Methods: The study population consisted of six healthy Standardbred mares in dioestrus. In each mare an acute endometritis was induced by performing a low- volume uterine flush 6days after ovulation using a sterile isotonic solution (lactated Ringer's solution or ViGro™ Complete Flush Solution). Two days after initiating inflammation, samples were collected from each ma...
Localization of Bovine papillomavirus in equine sarcoids and inflammatory skin conditions of horses using laser microdissection and two forms of DNA amplification. Wobeser BK, Hill JE, Jackson ML, Kidney BA, Mayer MN, Townsend HG, Allen AL.Equine sarcoids are the most common tumor of horses. Bovine papillomavirus (BPV) has been suggested as the cause of sarcoids. Studies have shown that BPV is present in swabs or biopsies from nonsarcoid-bearing equine skin. Skin biopsies from a variety of different conditions and normal skin from horses with no reported history of sarcoids were examined by polymerase chain reaction (PCR) for the presence of BPV, which was found in all different types of skin conditions as well as normal skin. Forty-one out of 86 skin biopsies from horses without sarcoids were found to contain BPV DNA. Laser mic...
Effect of sequential hole enlargement on cortical bone temperature during drilling of 6.2-mm-diameter transcortical holes in the third metacarpal bones of horse cadavers.
American journal of veterinary research    December 1, 2011   Volume 72, Issue 12 1687-1694 doi: 10.2460/ajvr.72.12.1687
Lescun TB, Frank EA, Zacharias JR, Daggy JK, Moore GE.To compare the bone temperature and final hole dimensions associated with sequential overdrilling (SO) and single 6.2-mm drill bit (S6.2DB) methods used to create transcortical holes in the third metacarpal bones (MCIIIs) of horse cadavers. Methods: 60 MCIIIs from 30 horse cadavers. Methods: In phase 1, hole diameter, tap insertion torque, peak bone temperature, and postdrilling bit temperature for 6.2-mm-diameter holes drilled in the lateral or medial cortical region of 12 MCIIIs via each of three 2-bit SO methods with a single pilot hole (diameter, 3.2, 4.5, or 5.5 mm) and the S6.2DB method ...