Equine Science encompasses the study of horses and their management, health, and performance. This field integrates various scientific disciplines such as biology, genetics, nutrition, physiology, and veterinary medicine to understand and improve the well-being and capabilities of horses. Areas of focus include equine anatomy, reproduction, behavior, and disease prevention. Research in equine science aims to enhance horse care, optimize training and performance, and address health challenges. This page compiles peer-reviewed research studies and scholarly articles that explore the diverse aspects of equine science, providing insights into the latest advancements and methodologies in the field.
Vergari C, Pourcelot P, Holden L, Ravary-Plumioën B, Laugier P, Mitton D, Crevier-Denoix N.Measure of the cross-sectional area (CSA) of biological specimens is a primary concern for many biomechanical tests. Different procedures are presented in literature but besides the fact that noncontact techniques are required during mechanical testing, most of these procedures lack accuracy or speed. Moreover, they often require a precise positioning of the specimen, which is not always feasible, and do not enable the measure of the same section during tension. The objective of this study was to design a noncontact, fast, and accurate device capable of acquiring CSA of specimens mounted on a ...
Masey O'Neill HV, Keen J, Dumbell L.The purpose of this study was to gain evidence on the prevalence of dental abnormalities in stable-kept horses in comparison with free-living horses. It is expected that free-living horses that graze for as much as 16 h/day will have fewer dental abnormalities than stable-kept horses. In this study, the latter group was fed a diet that was based on a relatively high-energy, cereal-based feed. This was thought to be a representative of common practice in domesticated, stable-kept horses. Compound diets such as this have previously been shown to increase the frequency of chewing cycles and decre...
Li XQ, Uboh CE, Soma LR, Guan FY, You YW, Kahler MC, Judy JA, Liu Y, Chen JW.A non-aqueous capillary electrophoresis-mass spectrometry (NACE-MS) method was developed for simultaneous separation and identification of 12 amphetamine and related compounds in equine plasma. Analytes were recovered from plasma by liquid-liquid extraction using methyl tertiary butyl ether (MTBE). A bare fused-silica capillary was used for separation of the analytes. Addition of sheath liquid to the capillary effluent allowed the detection of the analytes by positive electrospray ionization mass spectrometry using full scan data acquisition. The limit of detection (LOD) for the target analyte...
Reardon RJ, Bailey R, Walmsley JP, Heller J, Lischer C.To (1) define mechanical properties in flexion, extension, and left lateral bending of cadaveric equine 4th and 5th cervical (C4-C5) articulations, (2) compare biomechanical properties of C4-C5 when stabilized with a kerf cut cylinder (KCC) compared with a ventrally placed 4.5 mm locking compression plate (LCP). Methods: In vitro biomechanical investigation. Methods: Cadaveric adult equine cervical vertebral columns (n=54). Methods: Cervical vertebrae aged by horse dentition and size measured from radiographs were divided into 3 age groups then randomly allocated to 3 groups. The C4-C5 articul...
Liu Y, Uboh CE, Soma LR, Li X, Guan F, You Y, Rudy JA, Chen JW.In 2008, Pennsylvania (PA) became the first State in the USA to ban and enforce the ban on the use of anabolic and androgenic steroids (AAS) in equine athletes by using plasma for analysis. To enforce the ban, a rapid and high-throughput method for analysis of 60 AAS in equine plasma was developed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Analytes were recovered from plasma by liquid-liquid extraction (LLE) using methyl tert-butyl ether, separated on a reversed-phase C₁₈ column and analyzed by electrospray ionization mass spectrometry. Multiple-reaction monitoring (MRM)...
Oosterlinck M, Pille F, Back W, Dewulf J, Gasthuys F.The aim of this study was to evaluate the association between fore- and hind-hoof contact area and limb loading. Data from a previous study on forelimb loading and symmetry were compared with data on hindlimb kinetics, and the fore- and hind-hoof contact area at the walk and trot was evaluated. Five sound ponies, selected for symmetrical feet, were walked and trotted over a pressure plate embedded in a custom-made runway. The hindlimb peak vertical force (PVF) and vertical impulse (VI) were found to be significantly lower than in the forelimb, whereas their high symmetry ratios (>95%) did n...
Panzani D, Zicchino I, Taras A, Marmorini P, Crisci A, Rota A, Camillo F.Artificial photoperiod treatment is currently the best method to hasten the first ovulation of the breeding season in winter anoestrus mares. However, this is not easy to apply in large herds of mares and, to be effective, has to be planned in the northern hemisphere in December at the latest. Pharmacological treatments have been proposed as alternatives: GnRH agonists, progesterone or its synthetic agonist Altrenogest, and dopamino-antagonists, as pherphenazine, domperidone or sulpiride. Dopamino-antagonists protocols, beginning at a given day of the year, gave controversial results in terms ...
Elia JB, Erb HN, Houpt KA.The natural diet of free-ranging horses is grass, which is typically high in fiber and calorically dilute, however diets for high performance domestic horses are often low in fiber and calorically dense. The aim of the study was to determine the motivation of horses for hay when fed a low roughage diet. Their motivation could be used to determine if low roughage diets compromise the welfare of horses. Eight mares were fed two different diets in counterbalanced order: ad libitum orchard grass hay; a complete pelleted feed (pellets). Each trial lasted three weeks, with a one-week transition peri...
Pokrywka A, Gorczyca D, Jarek A, Kwiatkowska D.Alfons Bukowski (1858-1921) is commonly regarded as the pioneer of anti-doping research. In 1910, he developed a method to detect alkaloids in horse saliva. One hundred years later, this is a good moment to remember Bukowski, an outstanding Polish pharmacist, often mistakenly represented in world literature as a Russian chemist. It is also an occasion to mention that the real driving forces in the history of doping were events related to horse rivalry.
Donofrio G, Capocefalo A, Franceschi V, Morini G, Del Bue M, Conti V, Cavirani S, Grolli S.Adipose-Derived Stromal Cells have been shown to have multiple lineage differentiation properties and to be suitable for tissues regeneration in many degenerative processes. Their use has been proposed for the therapy of joint diseases and tendon injuries in the horse. In the present report the genetic manipulation of Equine Adipose-Derived Stromal Cells has been investigated. Results: Equine Adipose-Derived Stromal Cells were successfully virally transduced as well as transiently and stably transfected with appropriate parameters, without detrimental effect on their differentiation properties...
Park SB, Seo MS, Kang JG, Chae JS, Kang KS.Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation. Methods: AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation ...
Revold T, Larsen S, Ihler CF.Less than a third of Norwegian-Swedish Coldblooded Trotters (NSCTs) have started racing as three year olds since the year 2000 despite the fact that large sums are paid out as price-money in the three year season. Recruitment races are arranged by the Norwegian Trotting Association (NTA) to stimulate early training. The management of young horses varies considerably and a large majority is reared by amateurs. The aim of the present study was to identify predictors of early race starts in young NSCT horses under field conditions. Methods: Of the 801 registered NSCT horses born in 2005, 144 were...
Westermann CM, Dorland L, Wijnberg ID, de Sain-van der Velden MGM, van Breda E, Barneveld A, de Graaf-Roelfsema E, Keizer HA, van der Kolk JH.The objective of this study is to assess the influence of acute exercise, training and intensified training on the plasma amino acid profile. In a 32-week longitudinal study using 10 Standardbred horses, training was divided into four phases, including a phase of intensified training for five horses. At the end of each phase, a standardized exercise test, SET, was performed. Plasma amino acid concentrations before and after each SET were measured. Training significantly reduced mean plasma aspartic acid concentration, whereas exercise significantly increased the plasma concentrations of alanin...
Guest DJ, Smith MR, Allen WR.Injury to the superficial digital flexor tendon (SDFT) is common in racing and sport horses and poor tendon regeneration leads to high reinjury rates. Autologous mesenchymal stromal cells (MSCs) are being used clinically to improve tendon regeneration but they have some practical limitations. Embryonic stem cells (ESCs) may overcome these limitations but their fate following injection into the damaged SDFT is unknown. Objective: To inject MSCs and ESCs into distinct areas of damage in the SDFT and monitor their survival over a 3 month period. Methods: MSCs and ESCs expressing different reporte...
Dugdale AH, Curtis GC, Cripps P, Harris PA, Argo CM.Increased prevalence of obesity among UK horses and ponies demands evidence-based advice to promote weight loss. Objective: Restriction of dry matter intake (DMI) to 1% of body mass (BM, 67% [corrected] of predicted maintenance digestible energy [DE] requirements) would promote weight loss without compromise to health. Methods: Five mature (mean ± s.e. 10 ± 2 years), overweight/obese pony mares (BM, 257 ± 20 kg: body condition score [BCS] 6.8/9 ± 0.5) were studied over 12 weeks. Animals were individually housed. Daily provision of a chaff-based, complete diet (measured DE, 8.5 MJ/kg DM) wa...
Martin AM, Elliott JA, Duffy P, Blake CM, Ben Attia S, Katz LM, Browne JA, Gath V, McGivney BA, Hill EW, Murphy BA.Circadian rhythms are innate 24-h cycles in behavioral and biochemical processes that permit physiological anticipation of daily environmental changes. Elucidating the relationship between activity rhythms and circadian patterns of gene expression may contribute to improved human and equine athletic performance. Six healthy, untrained mares were studied to determine whether locomotor activity behavior and skeletal muscle gene expression reflect endogenous circadian regulation. Activity was recorded for three consecutive 48-h periods: as a group at pasture (P), and individually stabled under a ...
Krueger K, Flauger B, Farmer K, Maros K.This study evaluates the horse (Equus caballus) use of human local enhancement cues and reaction to human attention when making feeding decisions. The superior performance of dogs in observing human states of attention suggests this ability evolved with domestication. However, some species show an improved ability to read human cues through socialization and training. We observed 60 horses approach a bucket with feed in a three-way object-choice task when confronted with (a) an unfamiliar or (b) a familiar person in 4 different situations: (1) squatting behind the bucket, facing the horse (2) ...
Buss DG, Giuliano E, Sharma A, Mohan RR.To determine if hybrid adeno-associated virus serotype 2/5 (AAV5) vector can effectively deliver foreign genes into the equine cornea without causing adverse side effects. The aims of this study were to: (i) evaluate efficacy of AAV5 to deliver therapeutic genes into equine corneal fibroblasts (ECFs) using enhanced green fluorescent protein (EGFP) marker gene, and (ii) establish the safety of AAV5 vector for equine corneal gene therapy. Methods: Primary ECF cultures were harvested from healthy donor equine corneas. Cultures were maintained at 37°C in humidified atmosphere with 5% CO(2). Metho...
Choi YH, Gustafson-Seabury A, Velez IC, Hartman DL, Bliss S, Riera FL, Roldán JE, Chowdhary B, Hinrichs K.The equine embryo possesses a capsule that is considered essential for its survival. We assessed viability after breaching the capsule of early (Day 6) and expanded (Day 7 and 8) equine blastocysts by micromanipulation. The capsule was penetrated using a Piezo drill, and trophoblast biopsy samples were obtained for genetic analysis. Pregnancy rates for Day-6 embryos, which had intact zonae pellucidae at the time of recovery, were 3/3 for those biopsied immediately after recovery and 2/3 for those biopsied after being shipped overnight under warm (∼28 °C) conditions. The pregnancy rates for ...
Clutton RE, Chase-Topping M, Squires R, Lawson H, Minard H, Rose S.The dimensions of anaesthetised hobbled horses during suspension and transfer onto the operating table are unknown. These data are required for the cost-effective construction of equine surgical facilities. Objective: To measure the distance from the toe to dependent back margin (Bsusp) and poll (Psusp) of anaesthetised suspended horses and correlate them with readily obtained measures from standing animals. Methods: Digital photographs of suspended horses were taken in the anaesthesia induction box at a fixed position that allowed trigonometric determination of Bsusp and Psusp. These values w...
Gómez MD, Menendez-Buxadera A, Valera M, Molina A.A total of 71 522 records (from 3154 horses) with the times per kilometre (TPK), recorded in Spanish Trotter horses (individual races) from racing performances held from 1991 to 2007, were available for this study. The TPK values for the different age groups (young and adult horses) and different distances (1600-2700 m) were considered as different traits, and a bi character random regression model (RRM) was applied to estimate the (co)variance components throughout the trajectory of age groups and distances. The following effects were considered as fixed: the combination of hippodrome-dat...
Gurney SM, Schneider S, Pflugradt R, Barrett E, Forster AC, Brinkmann B, Jansen T, Forster P.Horse mtDNA profiling can be useful in forensic work investigating degraded samples, hair shafts or highly dilute samples. Degraded DNA often does not allow sequencing of fragments longer than 200 nucleotides. In this study we therefore search for the most discriminatory sections within the hypervariable horse mtDNA control region. Among a random sample of 39 horses, 32 different sequences were identified in a stretch of 921 nucleotides. The sequences were assigned to the published mtDNA types A-G, and to a newly labelled minor type H. The random match probability within the analysed samples i...
Ferreira-Dias G, Costa AS, Mateus L, Korzekwa AJ, Galvão A, Redmer DA, Lukasik K, Szóstek AZ, Woclawek-Potocka I, Skarzynski DJ.Cytokines and nitric oxide (NO) are potential mediators of luteal development and maintenance, angiogenesis, and blood flow. The aim of this study was to evaluate (i) the localization and protein expression of endothelial and inducible nitric oxide synthases (eNOS and iNOS) in equine corpora lutea (CL) throughout the luteal phase and (ii) the effect of a nitric oxide donor (spermine NONOate, NONOate) on the production of progesterone (P4) and prostaglandin (PG) E(2) and factor(s) that stimulate endothelial cell proliferation using equine luteal explants. Luteal tissue was classified as corpora...
Cissell JM, Milton SC, Dahlgren LA.To evaluate the effects of prostaglandin E₂ (PGE₂) treatment on the metabolism of equine tendon fibroblasts in vitro to aid in investigating the response of tendon fibroblasts to injury and novel therapeutics. Methods: Superficial digital flexor tendon fibroblasts isolated via collagenase digestion from six young adult horses were grown in monolayer in four concentrations of PGE₂ (0, 10, 50, 100 ng/ml) for 48 hours. Cells and medium were harvested for gene expression (collagen types I and III, cartilage oligomeric matrix protein [COMP], decorin, and matrix metalloproteinase-1, -3, and -1...
Ledbetter EC, Irby NL.To describe the corneal in vivo confocal microscopy (IVCM) findings in horses with putative immune-mediated keratitis (IMMK). Methods: Sixty five horses with IMMK. Methods: Horses diagnosed with IMMK were examined with a modified Heidelberg Retina Tomograph II and Rostock Cornea Module. The findings from the IVCM examinations were correlated with clinical details from ophthalmic examination and diagnostic test results. Results: Eighty eyes from 65 horses were examined. Clinical IMMK lesions were categorized as epithelial (n = 17 eyes), superficial stromal (n = 38), midstromal (n = 18), a...
Parks AH.Therapeutic shoeing is best directed at a specific diagnosis, but in the absence of a specific diagnosis, it is frequently directed at a symptom. There are only so many ways to modify the function of the foot with trimming and shoeing. The design of a horse shoe may often be modified to improve one aspect of foot function. Modifying a horse shoe to improve one aspect of foot function almost invariably impacts another aspect of foot function. The application of horse shoes may be based on a specific diagnosis or directed at a symptom. The application of shoeing principles is best approached usi...
Nakagawa K, Yamada Y, Fujiwara K, Ikeguchi M.Equine beta-lactoglobulin forms a compact intermediate at an acidic pH (A state). It also forms an expanded and helical conformation at low temperatures (C state). The structure of a single disulfide mutant C66A/C160A is similar to the A state in the presence of salts, while it is similar to the C state at low anion concentrations. We have investigated the temperature-dependent change in the secondary structure using circular dichroism and proline scanning mutagenesis. At low anion concentrations, the helical content increased linearly as temperature decreased. In the presence of salts, the A ...
Kamerling SG, Dequick DJ, Weckman TJ, Sprinkle FP, Tobin T.The effects of procaine, mepivacaine and phenylbutazone on pain perception in the equine were studied using two behavioral assays of nociception; the thermal evoked hoof withdrawal reflex and skin twitch reflex. Pain perception threshold was measured as the latency from onset of thermal stimuli to reflex withdrawal of the forelimb or contraction of the cutaneous musculature. Procaine 2% and mepivacaine 2% prolonged the hoof withdrawal reflex latency when administered locally by producing a block of the palmar and metacarpal nerves. Significant analgesia lasted 90 min and 210 min for procaine a...
Christmann U, Hite RD, Tan RH, Thatcher CD, Witonsky SG, Werre SR, Buechner-Maxwell VA.To evaluate the phospholipid composition and function of surfactant in horses with recurrent airway obstruction (RAO) at various clinical stages and compare these properties with findings in horses without RAO. Methods: 7 horses with confirmed RAO and 7 without RAO (non-RAO horses). Methods: Pairs of RAO-affected and non-RAO horses were evaluated before, during, and after exposure to hay. Evaluations included clinical scoring, lung function testing, airway endoscopy, and bronchoalveolar lavage fluid (BALF) absolute and differential cell counts. Cell-free BALF was separated into crude surfactan...
Song K, Yang GM, Han J, Gil M, Dayem AA, Kim K, Lim KM, Kang GH, Kim S, Jang SB, Vellingiri B, Cho SG.Flavonoids form the largest group of plant phenols and have various biological and pharmacological activities. In this study, we investigated the effect of a flavonoid, 3, 4'-dihydroxyflavone (3, 4'-DHF) on osteogenic differentiation of equine adipose-derived stromal cells (eADSCs). Unassigned: Treatment of 3, 4'-DHF led to increased osteogenic differentiation of eADSCs by increasing phosphorylation of ERK and modulating Reactive Oxygen Species (ROS) generation. Although PD98059, an ERK inhibitor, suppressed osteogenic differentiation, another ERK inhibitor, U0126, apparently increased osteoge...
Gim JA, Lee S, Kim DS, Jeong KS, Hong CP, Bae JH, Moon JW, Choi YS, Cho BW, Cho HG, Bhak J, Kim HS.With the advent of next-generation sequencing technology, genome-wide maps of DNA methylation are now available. The Thoroughbred horse is bred for racing, while the Jeju horse is a traditional Korean horse bred for racing or food. The methylation profiles of equine organs may provide genomic clues underlying their athletic traits. We have developed a database to elucidate genome-wide DNA methylation patterns of the cerebrum, lung, heart, and skeletal muscle from Thoroughbred and Jeju horses. Using MeDIP-Seq, our database provides information regarding significantly enriched methylated regions...
Padua MB, Kowalski AA, Cañas MY, Hansen PJ.Uterine serpins (USs), designated as SERPINA14, are expressed in the endometrium in response to progesterone. All species identified as having USs exhibit epitheliochorial placentation and are in the Ruminantia and Suidae orders of the Laurasiatheria superorder. The objective was to identify US genes in species within and outside Laurasiatheria and evaluate whether evolution of the US gene was associated with development of the epitheliochorial placenta. Through queries of nucleotide and genomic databases, known US genes were identified (caprine, bovine, porcine, water buffalo), and new US cod...
Herbrecht V, Waldern NM, Mikkelsen SE, Kjaer M, Dittmann MT, Wiestner T, Weishaupt MA.In order to improve the four-beat-rhythm and forelimb action of the tölt in Icelandic horses (ICE), special shoeing techniques are applied particularly in the front hooves by increasing hoof length and height, or by adding weight to the distal limbs. Although regulations limit dorsal hoof wall length (LDHW) in competition ICE, their shoeing often deviates from a biomechanically optimal distal limb conformation. This study aimed to qualitatively and quantitatively describe current shoeing practices of ICE in competition. Moreover, the influence of LDHW on the occurrence of deviations from a st...
Ahmed BM, Bayoumi MM, Farrag MA, Elgamal MA, Daly JM, Amer HM.Equine influenza is an important cause of respiratory disease in equids. The causative virus; EIV, is highly variable and can evolve by accumulation of mutations, particularly in the haemagglutinin (HA) gene. Currently, H3N8 is the sole subtype circulating worldwide with Florida clade 1 (FC1) is most prevalent in the Americas and FC2 in Asia and Europe. In Egypt, EIV was detected in two occasions: subtype H7N7 in 1989 and subtype H3N8 (FC1) in 2008. No data is available on the circulation pattern of EIV during the last decade despite frequent observation of suspected cases. Twenty-two nasal sw...
Greco-Otto P, Massie S, Shields E, Roy MF, Pajor E, Léguillette R.The Heavy Horse Pull is a competition where teams of two horses pull an increasingly heavy sled for a short distance. Similar to human wrestlers, some horses may undergo rapid weight change in order to enter a lower weight category. The objectives were to study the physiological effects of this practice as well as of the pulling competition in draft horses. Results: Fifty horses were divided into light-, middle- and heavyweight categories based on their arrival weights and competed 1-3 days after. Body weight was measured upon arrival and pre-competition. Blood was sampled for chemistry and h...
McDowell KJ, Webb BA, Williams NM, Donahue JM, Newman KE, Lindemann MD, Horohov DW.A new abortigenic disease, now known as mare reproductive loss syndrome (MRLS), significantly affected the horse industry in the Ohio River Valley of the United States in late April and early May of 2001 and 2002. In 2001, approximately 25% of all pregnant mares aborted within several weeks (over 3,000 mares lost pregnancies), and abortion rates exceeded 60% on some farms. Mare reproductive loss syndrome struck hard and without warning, it was caused by something in the environment, it was not transmitted between animals, and it was not associated with any known abortigenic agent or disease. T...
Lepage OM, Doumbia A, Perron-Lepage MF, Gangl M.Maggot debridement therapy is a long-established tool to promote wound healing. Objective: To describe and assess the results of this technique in equids with various lesions. Methods: Retrospective analysis performed on cases in which, depending on clinical case, type, size and location of the wound, maggots were applied either in direct or indirect contact with the wound. Results: Treated cases (n = 41) included horses with foot pathology (n = 9), laceration of the limbs (n = 15), other soft tissue abscesses or wounds (n = 6), fistulous withers (n = 5), other musculoskeletal infection (n = 2...
Perez-Jimenez EE, Biedrzycki AH, Morton AJ, McCarrel TM.To determine the influence of a custom 3D-printed guide for placement of cortical bone screws in the equine navicular bone. Methods: Ex vivo study. Methods: Eight pairs of normal adult equine forelimbs. Methods: A 3.5 × 55 mm cortical screw was placed in the longitudinal axis of each intact navicular bone. Screws were placed with a 3D-printed guide (3D) in one bone and with a traditional aiming device (AD) in the contralateral bone within each pair. Duration of surgery and the number of fluoroscopy images were compared between techniques. Screw placement was subjectively evaluated by gros...
Rouse BT, Ditchfield WJ.Nasal secretions and serum were collected from ponies in order to determine the types of immunoglobulins responsible for antibody activity against Myxovirus influenzae A-equi 2. Using specific antisera to remove each immunoglobulin, the antiviral activity of serum was shown to be a property of IgG globulin, whereas in nasal secretions activity was found in both IgG and IgG(T) globulins. However in secretions the predominant activity was attributed to IgG(T) globulin. Because of its sensitivity to 2 mercaptoethanol, early serum antibody was assumed to belong to the IgM class of immunoglobulins....
Kümmerle JM, Kummer MR.To describe the arthroscopically accessible anatomy of the tarsal collateral ligaments in the horse. Methods: Descriptive study. Methods: Cadaveric equine hind limbs (n = 24) obtained from horses without tarsal disease. Methods: Two pairs of tarsal joints were used to obtain silicone models of the tarsocrural joint and dissect the tarsal collateral ligaments (CLs). Ten pairs of tarsocrural joints had arthroscopic exploration and the accessible parts of the tarsal CLs were marked with an arthroscopic hook knife. Subsequently, the limbs were dissected and the marked positions were allocated to t...
Vinogradov E, MacLean LL, Brooks BW, Lutze-Wallace C, Perry MB.Taylorella asinigenitalis sp. nov is a nonpathogenic gram-negative bacterium recently isolated from the genital tract of male donkeys. The bacterium is phenotypically indistinguishable from Taylorella equigenitalis, a pathogen that is the cause of contagious equine metritis, a highly communicable venereal disease of horses. The structural analysis of the lipopolysaccharide produced by T. asinigenitalis sp. nov (ATCC 700933) demonstrated that its O-polysaccharide (O-PS) component is a linear unbranched polymer of repeating disaccharide units composed of 1,3-linked pyranosyl residues of 2,4-diac...
Radtke A, Fortier LA, Regan S, Kraus S, Delco ML.Equine diagnostic anaesthesia can be a useful tool in challenging lameness examinations. However, anaesthetics diffuse over time leading to nonspecific desensitisation of periarticular structures. Nerves that convey sensation from the distal limb to the central nervous system pass in close proximity to the caudal stifle joint capsule. Therefore, diffusion of intra-articular (IA) anaesthetics could cause inadvertent desensitisation of the distal limb resulting in a false diagnosis of stifle lameness. Objective: To determine if IA stifle anaesthesia can alleviate lameness originating in the dist...
Morel PC, Bokor A, Rogers CW, Firth EC.To mathematically describe the growth of a population of Thoroughbred foals reared on pasture in New Zealand from birth to weaning. Methods: Twice-monthly liveweight data (including birthweight; BW) from 218 foals (98 colts and 120 fillies) born over a 6-year period, and reared on four different pasture types, were available for analysis. Data truncated to the time of weaning (107-217 days old) provided 3,200 data points. After testing for non-linearity of the relationship of age and liveweight (LW), five non-linear regression equations were tested for goodness of fit. Results: Colts and filli...
O'Leary SA, White JL, Hu JC, Athanasiou KA.The equine cervical facet joint is a site of significant pathology. Located bilaterally on the dorsal spine, these diarthrodial joints work in conjunction with the intervertebral disc to facilitate appropriate spinal motion. Despite the high prevalence of pathology in this joint, the facet joint is understudied and thus lacking in viable treatment options. Objective: The goal of this study was to characterise equine facet joint cartilage and provide a comprehensive database describing the morphological, histological, biochemical and biomechanical properties of this tissue. Methods: Descriptive...
Beekley MD, Ideus JM, Brechue WF, Kearns CF, McKeever KH.The purpose of this study was to examine changes in myosin heavy chain (MHC) composition due to chronic clenbuterol administration with or without exercise in mares. Unfit Standardbred mares (aged 10+/-3 years) were divided into four groups: clenbuterol (2.4 micro/kg BW twice daily) plus exercise (3 days/week for 20 min at 50% VO(2max); CLENEX; n=6), clenbuterol only (CLEN; n=6), exercise only (EX; n=5), and control (CON; n=6). Muscle biopsies were obtained from gluteus medius muscle before and after the eight-week training/administration period. MHC composition was determined via SDS gel elec...
Davis MS, Royer CM, McKenzie EC, Williamson KK, Payton M, Marlin D.Inspired air is warmed to body temperature and fully humidified by the upper airway mucosa under normal resting conditions. This conditioning process may not be completed by the upper airways during conditions of increased minute ventilation or when the inspired air is unusually cold, resulting in cooling and desiccation of lower respiratory surfaces. Excess heat and water loss from intrapulmonary airways is believed to be the provocative stimulus for exercise-induced bronchoconstriction (occurring immediately after exercise) and associated late phase airway obstruction (occurring a few hours ...
Tavanaeimanesh H, Dashli-Boroon OJ, Corley K.Understanding the physiological and biochemical changes in racehorses can be invaluable. Accurate information in this area could result in better understanding of needs of sport horses. The aim of this study was to prove the hypothesis that biochemical changes could influence the outcome of competitions. In this study, β-endorphin was evaluated as an indicator of analgesia, lactate as an indicator of fatigue and cortisol as an indicator of stress in the first two horses and the last ones that cross the finish line. This study was performed on 44 horses participating in the 1000-meter national...
Mesoangioblasts (MABs) are vessel-associated stem cells that express pericyte markers and are originally isolated from the embryonic dorsal aorta. From postnatal small vessels of skeletal muscle and heart, it is possible to isolate cells with similar characteristics to embryonic MABs. Adult MABs have the capacity to self-renew and to differentiate into cell types of mesodermal lineages upon proper culture conditions. To date, the origin of MABs and the relationship with other muscle stem cells are still debated. Recently, in a phase I-II clinical trial, intra-arterial HLA-matched MABs were pro...
Hartmann E, Hopkins RJ, von Brömssen C, Dahlborn K.Provision of shelter for horses kept on summer pasture is rarely considered in welfare guidelines, perhaps because the benefits of shelter in warm conditions are poorly documented scientifically. For cattle, shade is a valued resource during summer and can mitigate the adverse effects of warm weather on well-being and performance. We found in a previous study that horses utilized shelters frequently in summer. A shelter with a roof and closed on three sides (shelter A) was preferred and can reduce insect pressure whereas a shelter with roof and open on three sides was not utilized. However, sh...
Janczarek I, Wilk I, Zalewska E, Bocian K.The aim of this paper was to select atmospheric factors and their values, which may disrupt the correct behavior and physiological condition of recreational horses. The studies were carried out from 1 July until 1 September on 16 Anglo-Arabian geldings. Each day, from 09.00 to 10.00 hours, the horses worked under saddle. The riders and the authors gave a qualitative behavioral assessment for each horse. Mood and willingness to work were evaluated. The quantitative assessment was called 'incorrect behavior of the horse while riding' (IBHR). The percentage time of duration and the number of occu...
Stowe HD.Serum and milk samples from mares and serum samples from their foals were taken at parturition and on d 1, 2, 4, 7, 14 and 21 postpartum. The samples were assayed for retinyl (r.) palmitate, r. acetate and retinol by high performance liquid chromatography. Peak vitamin A activity in milk occurred 1 d postpartum and preceded by 3 d the maximum vitamin A activity in foal serum and the lowest vitamin A activity in the mare serum. Mare serum contained approximately a 65:35 ratio of retinol:r. palmitate and less than 1% r. acetate. Retinyl palmitate was the predominant form of vitamin A in milk unt...
D'Fonseca NMM, Gibson CME, Hummel I, van Doorn DA, Roelfsema E, Stout TAE, van den Broek J, de Ruijter-Villani M.Obesity has been associated with altered reproductive activity in mares, and may negatively affect fertility. To examine the influence of long-term high-energy (HE) feeding on fertility, Shetland pony mares were fed a diet containing 200% of net energy (NE) requirements during a three-year study. The incidence of hemorrhagic anovulatory follicles (HAF) and annual duration of cyclicity were compared to those in control mares receiving a maintenance diet. Day-7 embryos were flushed and transferred between donor and recipient mares from both groups; the resulting conceptuses were collected 21 day...
Rendle DI, Heller J, Hughes KJ, Innocent GT, Durham AE.Time delays between collection of blood samples and biochemical analysis of equine blood are unavoidably common in equine practice. The effect that delays may have on the accuracy of results of blood biochemical analyses is not well established. Objective: Delays in processing of blood of up to 72 h results in alterations in measured levels of common biochemical analytes that are of potential clinical relevance. Separation of serum prior to storage is protective against the effects of time delays. Methods: Samples of clotted blood, separated serum and oxalate fluoride plasma from 20 horses wer...
Holmström M, Drevemo S.The angular velocities of the hindlimb angles of 14 horses, including 6 Grand Prix dressage horses, 4 horses judged as good at the trot and 4 horses judged as poor, were analysed. The horse material was the same as previously used by Holmström (1994) in studies on conformation and trotting gaits in the Swedish Warmblood riding horse. Four consecutive strides of each horse and the corresponding pace were analysed and mean velocity curves (Xh) for each angle were calculated. Before calculation the data were filtered forwards and backwards with a Butterworth third order filter with a cut off fre...
