Analyze Diet

Topic:Feed Intake

Feed intake in horses refers to the amount and type of feed consumed by horses, which can vary based on factors such as age, activity level, health status, and environmental conditions. It is a critical aspect of equine management, influencing growth, performance, reproduction, and overall health. Feed intake can be affected by the nutritional content of the diet, palatability, feeding practices, and physiological needs of the horse. Understanding feed intake patterns and requirements is essential for formulating appropriate feeding strategies. This page compiles peer-reviewed research studies and scholarly articles that explore the determinants of feed intake, its impact on equine physiology, and strategies for optimizing feeding practices in horses.
Safety and efficacy of a feed additive consisting of glyceryl polyethyleneglycol ricinoleate (PEG castor oil) for all animal species (FEFANA asbl).
EFSA journal. European Food Safety Authority    October 28, 2022   Volume 20, Issue 10 e07433 doi: 10.2903/j.efsa.2022.7433
Bampidis V, Azimonti G, Bastos ML, Christensen H, Dusemund B, Fašmon Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B....Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of glyceryl polyethyleneglycol ricinoleate (PEG castor oil) as technological feed additive for all animal species. PEG castor oil is safe at a maximum concentration in complete feed of 90 mg/kg for chickens for fattening and other minor growing poultry; 134 mg/kg for laying hens and other laying/breeding birds kept for egg production/reproduction; 121 mg/kg for turkeys for fattening; 162 mg/kg for piglets and other minor growing ; 194 mg/kg for pigs for fattening; 23...
Seasonal Changes in Trace-Element Content in the Coat of Hucul Horses.
Animals : an open access journal from MDPI    October 14, 2022   Volume 12, Issue 20 doi: 10.3390/ani12202770
Jachimowicz-Rogowska K, Topczewska J, Krupa W, Bajcar M, Kwiecień M, Winiarska-Mieczan A.The purpose of the study was to evaluate seasonal changes in selected trace elements such as Fe, Cu, Mn, Zn, and Al in the coat of healthy Hucul horses kept in south-eastern Poland in two different facilities and fed with locally sourced feed. The coat for the study was collected from 24 individuals in autumn, winter, and spring. The concentration of elements in the feed was also determined. The date of collection had a significant effect on the concentration of the micronutrients analysed in the coat of Hucul horses. The highest concentration of Zn was found in the coat taken in summer. The c...
[Is alfalfa hay an alternative to meadow hay in equine nutrition?].
Schweizer Archiv fur Tierheilkunde    October 5, 2022   Volume 164, Issue 10 721-731 doi: 10.17236/sat00371
Köninger M, von Velsen-Zerweck A, Eiberger C, Jilg A, Töpper A, Visscher C, Reckels B, Vervuert I.Roughage alternatives for equine nutrition must be found due to the increasing climatic challenges. The aim of this study was to examine the nutrient composition and feed hygiene of alfalfa hay in comparison to meadow hay and to investigate the acceptance, weight development and faecal quality of broodmares fed alfalfa hay. The feed used was examined for nutrient content and hygiene status according to the standards of the Verband Deutscher Landwirtschaftlicher Untersuchungs- und Forschungsanstalten (VDLUFA). Twenty adult pregnant warmblood mares were fed in two groups (n=10) with a defined am...
Animal-assisted therapy in eating disorder treatment: A systematic review.
Eating behaviors    September 30, 2022   Volume 47 101673 doi: 10.1016/j.eatbeh.2022.101673
Fennig MW, Weber E, Santos B, Fitzsimmons-Craft EE, Wilfley DE.Eating disorders (EDs) are deadly illnesses with high relapse rates, highlighting need for better interventions. Animal-assisted therapy (AAT) has been implemented supplementally for EDs, with horses utilized at many residential facilities. AAT shows promise with meta-analyses of randomized control trials (RCTs) showing significant decreases in depression, anxiety, and negative affect; however, no review to date has evaluated efficacy for EDs. Therefore, this study conducted a systematic review of primary literature to investigate the efficacy of AAT for EDs. A systematic review was conducted ...
Assessment of information as regards the toxicity of fumonisins for pigs, poultry and horses.
EFSA journal. European Food Safety Authority    August 24, 2022   Volume 20, Issue 8 e07534 doi: 10.2903/j.efsa.2022.7534
Schrenk D, Bignami M, Bodin L, Chipman JK, Del Mazo J, Grasl-Kraupp B, Hogstrand C, Leblanc JC, Nielsen E, Ntzani E, Petersen A, Sand S, Schwerdtle T....In 2018, the EFSA Panel on Contaminants in the Food Chain (CONTAM) adopted a Scientific Opinion on the risks for animal health related to the presence of fumonisins, their modified forms and hidden forms in feed. A no observed adverse effect level (NOAEL) of 1 mg/kg feed was established for pigs. In poultry a NOAEL of 20 mg/kg feed and in horses a reference point for adverse animal health effect of 8.8 mg/kg feed was established, referred to as NOAEL. The European Commission (EC) requested EFSA to review the information regarding the toxicity of fumonisins for pigs, poultry and horses an...
The Microbiological Quality of Concentrates for Horses-A Retrospective Study on Influencing Factors and Associations with Clinical Symptoms Reported by Owners or Referring Vets.
Veterinary sciences    August 5, 2022   Volume 9, Issue 8 413 doi: 10.3390/vetsci9080413
Intemann S, Reckels B, Schubert DC, Wolf P, Kamphues J, Visscher C.Evidence has already been provided that feed-borne mold spores and endotoxins can trigger chronic, non-infectious respiratory disease if inhaled. Furthermore, deficiencies in feed microbiology are suspected to trigger gastrointestinal and liver disorders in horses, but the connection needs further clarification. Most of the previous studies regarding horse feed hygiene focused on forage, whereas research regarding hygienic quality of concentrates is scarce. In the present study, results of reports on hygienic quality of compound feed and cereals for horses were evaluated secondarily. Results i...
Short communication: Utilisation of n-alkanes to estimate feed intake in horses fed known amounts of a labelled concentrate supplement.
Animal : an international journal of animal bioscience    June 22, 2022   Volume 16, Issue 7 100571 doi: 10.1016/j.animal.2022.100571
Maxfield M, Celaya R, Andrade E, Rodrigues MAM, Santos AS, Ferreira LMM.The assessment of feed intake in stabled horses is a difficult task to accomplish. Faecal markers, namely n-alkanes, have been used successfully for the estimation of this important nutritional parameter. This usually involves the dosing of synthetic n-alkanes via different matrices, a laborious task that may also influence the animal normal foraging behaviour. An experiment was conducted to evaluate a relative simple methodology to quantify feed intake in horses, based on the provision of measured amounts of a concentrate supplement labelled with beeswax and the utilisation of n-alkanes as fa...
Effects of Bromocriptine on Glucose and Insulin Dynamics in Normal and Insulin Dysregulated Horses.
Frontiers in veterinary science    May 31, 2022   Volume 9 889888 doi: 10.3389/fvets.2022.889888
Loos CMM, Urschel KL, Vanzant ES, Oberhaus EL, Bohannan AD, Klotz JL, McLeod KR.The objectives of the study were to study the effects of the synthetic ergot alkaloid (EA), bromocriptine, on glucose and lipid metabolism in insulin dysregulated (ID, n = 7) and non-ID (n = 8) mares. Horses were individually housed and fed timothy grass hay and two daily concentrate meals so that the total diet provided 120% of daily DE requirements for maintenance. All horses were given intramuscular bromocriptine injections (0.1 mg/kg BW) every 3 days for 14 days. Before and after 14 days of treatment horses underwent a combined glucose-insulin tolerance test (CGIT) to assess insulin sensit...
Horses’ Response to a Novel Diet: Different Herbs Added to Dry, Wet or Wet-Sweetened Oats.
Animals : an open access journal from MDPI    May 24, 2022   Volume 12, Issue 11 1334 doi: 10.3390/ani12111334
Stachurska A, Tkaczyk E, Różańska-Boczula M, Janicka W, Janczarek I.The commercial horse feed industry uses palatants to mask undesirable tastes of feeds and enhance product acceptance. However, an unknown odour or taste may also hinder feed intake, due to, inter alia, novelty. The objective of the study was to assess the horses' response to novel diet: five different herbs added alternately to dry, wet or wet-sweetened oats. Twenty adult horses were given different diet combinations of a feed presentation and a herb: field mint, common yarrow, common chamomile, common sage and common nettle, consecutively, once daily. The response to novelty was assessed rega...
Sporadic worldwide “clusters” of feed driven Zilpaterol identifications in racing horses: a review and analysis.
Irish veterinary journal    May 14, 2022   Volume 75, Issue 1 11 doi: 10.1186/s13620-022-00215-8
Machin J, Brewer K, Morales-Briceno A, Fenger C, Maylin G, Tobin T.Zilpaterol is a β2-adrenergic agonist medication approved in certain countries as a cattle feed additive to improve carcass quality. Trace amounts of Zilpaterol can transfer to horse feed, yielding equine urinary "identifications" of Zilpaterol. These "identifications" occur because Zilpaterol is highly bioavailable in horses, resistant to biotransformation and excreted as unchanged Zilpaterol in urine, where it has a 5 day or so terminal half-life.In horses, urinary steady-state concentrations are reached 25 days (5 half-lives) after exposure to contaminated feed. Zilpaterol readily presen...
The Hygienic Status of Different Forage Types for Horses-A Retrospective Study on Influencing Factors and Associations with Anamnestic Reports.
Veterinary sciences    May 6, 2022   Volume 9, Issue 5 doi: 10.3390/vetsci9050226
Intemann S, Reckels B, Schubert D, Wolf P, Kamphues J, Visscher C.The hygienic quality of forage for horses is discussed as a potential health hazard, especially regarding respiratory diseases, colic, and hepatopathies. Therefore, the purpose of this study was to investigate the possible relations between microbiological counts, as well as endotoxin levels and disease symptoms. Data from microbiological examination reports were analyzed retrospectively, including the results of sensory examination, microbiological counts, and lipopolysaccharide contents. Sensory analysis gave an indication of deficiencies in microbiological analysis, but both methods did not...
Correction: Saastamoinen et al. Protein Source and Intake Effects on Diet Digestibility and N Excretion in Horses-A Risk of Environmental N Load of Horses. Animals 2021, 11, 3568.
Animals : an open access journal from MDPI    March 28, 2022   Volume 12, Issue 7 doi: 10.3390/ani12070848
Saastamoinen M, Särkijärvi S, Suomala H.There was an error in the original publication [...].
The In Vitro Digestion and Fermentation Characteristics of Feedstuffs Inoculated With Cecal or Colic Fluid of Dezhou Donkey.
Journal of equine veterinary science    January 10, 2022   Volume 110 103864 doi: 10.1016/j.jevs.2022.103864
Zhang Z, Wang Y, Zhu M, Wang C.The present study was conducted to evaluate the in vitro dry matter disappearance (IVDMD) and fermentation characteristics of 6 fibrous feedstuffs incubated with donkey cecal or colic microorganisms. The fibrous feeds were corn straw, wheat straw, peanut vine, peanut shell, wheat shell and wheat bran (WB), which are commonly applied in large-scale donkey farms in China. After 48 hours fermentation, the highest IVDMD occurred in WB, and the lowest occurred in peanut shell (P < .05) regardless what inoculum applied. The IVDMD was positively correlated with OM (R = 0.42, P < .01), CP (R ...
Digestibility and nitrogen and water balance in horses fed rhizoma peanut hay.
Journal of animal science    October 12, 2021   Volume 99, Issue 11 doi: 10.1093/jas/skab284
Vasco ACCM, Brinkley-Bissinger KJ, Bobel JM, Dubeux JCB, Warren LK, Wickens CL.Rhizoma peanut (RP, Arachis glabrata) hay has the potential to meet horses' crude protein (CP) requirements with less nitrogen excretion than other legumes. This study aimed to evaluate nutrient intake, apparent digestibility, and nitrogen balance of RP "Florigraze" hay compared with alfalfa (ALF, Medicago sativa L. "Legendary XHD") and bermudagrass (BG, Cynodon dactylon L. "Coastal") hays when fed to maintenance horses at 2% body weight/d on a dry matter (DM) basis. We hypothesized that nutrient intake would be comparable between the legume hays and lesser for BG and that RP would result in r...
Feed Intake Parameters of Horses Fed Soaked or Steamed Hay and Hygienic Quality of Hay Stored following Treatment.
Animals : an open access journal from MDPI    September 18, 2021   Volume 11, Issue 9 2729 doi: 10.3390/ani11092729
Glatter M, Bochnia M, Wensch-Dorendorf M, Greef JM, Zeyner A.Horses suffering from equine asthma must consume low-dust forage, with soaking and steaming being suitable methods of hay treatment. The impacts of this treated hay's subsequent storage and effects on the horses' chewing activity are largely unknown. Meadow hay was soaked (10-15 °C, 15 min) or steamed (100 °C, 60 min). Microbial counts (colony forming units (CFU)) were determined by culture before and after soaking or steaming, and subsequent storage at 10 and 25 °C for 6, 12 and 24 h (three replicates each). Six horses were fed native, soaked and steamed hay, according to a cross-over desi...
Use of palm bran (Nopalea cochenillifera (L.) Salm-Dyck) in partial replacement of concentrate in maintenance equine diets – a pilot study.
Archives animal breeding    June 21, 2021   Volume 64, Issue 1 273-282 doi: 10.5194/aab-64-273-2021
Rodrigues PG, Garcez DSM, Silva CM, Santana CCS, Santana JCS, Lopes CDC, Muniz EN, de Oliveira Júnior GM, Silva de Moura R, de Souza JC.Forage palm is extremely suitable as animal fodder due to its high tolerance to the climatic rigors of the semiarid region and its ability to withstand the harsh physical-chemical limitations of poor soils. Thus, in this study, the effects of the partial replacement (0 %, 5 %, 10 % and 15 % replacement) of a molasses- or oat-based commercial concentrate with forage palm bran (FPB) on the acceptability, apparent digestibility and glycemic response of horses at maintenance were evaluated. The ratio of concentrate to roughage (Tifton 85 hay) was , and the dry matter (DM) intake was 2 %...
Effects of crude protein content on intake and digestion of coastal bermudagrass hay by horses.
Translational animal science    April 29, 2021   Volume 5, Issue 3 txab073 doi: 10.1093/tas/txab073
Spurgin CL, Coverdale JA, Leatherwood JL, Redmon LA, Bradbery AN, Wickersham TA.This study was conducted to determine the effects of forage crude protein () level on intake and digestion of Coastal bermudagrass hay by horses. Four cecally fistulated geldings were used in a 4 × 4 Latin square design with four treatments and four periods. Horses were fed one of four Coastal bermudagrass hays consisting of 7%, 10%, 13%, or 16% CP during each of the four 15-d periods. Intake and apparent digestibility were determined for each horse at the end of each period by total fecal collection. In addition, cecal fluid and blood samples were collected on the last day of each period for...
Effects of a nutraceutical supplement on gastrointestinal health in racing standardbreds.
Journal of animal physiology and animal nutrition    March 6, 2021   Volume 105, Issue 3 558-568 doi: 10.1111/jpn.13508
Wellard L, Medyk K, Pearson W.Feed additives that accelerate gastrointestinal transit time may help support normal gastrointestinal function in horses at risk for impaction colic. Previous research has demonstrated significant stimulatory effect of a hemp-based nutraceutical product (Gs Formula; GF) on contractility of gastric smooth muscle and gastrin production in vitro. Objective: To quantify effects of GF on indicators of GIT transit time and tight junction proteins. Methods: Randomized placebo-controlled cross-over study. Methods: Eight Standardbreds were administered 200 plastic beads by nasogastric tube before (base...
Effect of Feed Intake on Water Consumption in Horses: Relevance to Maintenance Fluid Therapy.
Frontiers in veterinary science    March 1, 2021   Volume 8 626081 doi: 10.3389/fvets.2021.626081
Freeman DE.Maintenance fluid therapy is challenging in horses that cannot drink or are denied feed and water because of concerns about gastrointestinal tract function and patency. Intravenous fluid delivery to meet water needs based on current recommendations for maintenance requirements were obtained in fed horses and therefore might not apply to horses that are not being fed. This is a critical flaw because of the interdependence between intestinal tract water and extracellular water to support digestion while preserving water balance, a concept explained by the enterosystemic cycle. Because horses dri...
The Positive Impact of Increasing Feeding Frequency on Feed Intake, Nutrient Digestibility, and Blood Metabolites of Turkmen Horses.
Journal of equine veterinary science    January 18, 2021   Volume 98 103390 doi: 10.1016/j.jevs.2021.103390
Direkvandi E, Rouzbehan Y, Fazaeli H.To determine the effects of feeding frequency on nutrient digestibility and blood metabolites in horse, 16 Turkmen horses were used with weight of 430 ± 46 kg and age of 7 ± 1.8 years. Four feeding frequencies with 2 (M2), 4 (M4), 6 (M6), and 8 (M8) meals per day were used for a period of 28 days. The ratio of hay to concentrate was 70:30 in the experimental diet. Acid insoluble ash was used as internal marker to determine digestibility. On the 27th day of experiment, the blood was collected on 13 time points throughout the day. The results of this experiment showed that feed intake was not ...
Introduction to automatic forage stations and measurement of forage intake rate in an active open barn for horses.
Animal : an international journal of animal bioscience    December 14, 2020   Volume 15, Issue 3 100152 doi: 10.1016/j.animal.2020.100152
Kjellberg L, Morgan K.Interest in the use of open barns on Swedish horse farms is increasing as an alternative to keeping horses in box stalls and as a 2007 law requires phasing out of tie stalls. To provide adequate forage to satisfy welfare requirements for nutrition, gut health and behavioural needs, the use of automated feeding is also increasing. Studies on forage intake rate report wide variation but provide little information on how to introduce horses to an automatic forage station and on how forage intake rate varies in individual horses fed using an automatic forage station. This study documented the proc...
The effects of processing barley and maize on metabolic and digestive responses in horses.
Journal of animal science    November 6, 2020   Volume 98, Issue 12 skaa353 doi: 10.1093/jas/skaa353
Thorringer NW, Weisberg MR, Jensen RB.The competition for customers increases the search for new grain processing methods for equine feed, but the effect on starch digestibility and metabolic responses varies. Therefore, to evaluate the effect of the processing methods, toasting and micronizing, on starch digestion and the effect on metabolic responses, the mobile bag technique (MBT) and plasma glucose and insulin concentrations in the blood were used to estimate nutrient disappearance and metabolic responses pre-cecally. Further, cecal pH, ammonium nitrogen (N), and short-chain fatty acid (SCFA) concentrations were used to estima...
The energy requirements of racehorses in training.
Translational animal science    November 3, 2020   Volume 4, Issue 4 txaa196 doi: 10.1093/tas/txaa196
Ebert M, Moore-Colyer MJS.The aim of this study was to estimate the energy requirements of Thoroughbred racehorses in active training for flat racing. Twenty-two Thoroughbred racehorses in England were measured over periods from 6 to 15 wk, which included periods of active race training and temporarily reduced training. Energy intake was determined by measuring daily feed consumption. Energy output was measured using heart rate monitors during 730 training sessions, relating heart rate (HR) to oxygen consumption (VO2) and converting VO2 to energy. Field maintenance requirements were calculated by deducting the marginal...
Dry Matter Intake, Body Weight, and Body Condition Scores of Blanketed and Nonblanketed Horses in the Upper Midwest.
Journal of equine veterinary science    August 29, 2020   Volume 94 103239 doi: 10.1016/j.jevs.2020.103239
DeBoer M, Konop A, Fisher B, Martinson K.Thermoregulation is an energy-expensive process, which can be mitigated by blanketing horses in cold climates, potentially preventing weight loss or leading to decreased feed intake. The objective of this study was to evaluate feed intake, body weight (BW), and body condition scores (BCSs) in blanketed and nonblanketed horses. In October 2019, 16 mature adult horses were blocked by breed and BCS and randomly assigned to a blanketed (n = 8) or nonblanketed (n = 8) treatment; blankets were placed. Data were collected in December 2019 and January 2020 in River Falls, Wisconsin. During the study...
Safety and efficacy of turmeric extract, turmeric oil, turmeric oleoresin and turmeric tincture from Curcuma longa L. rhizome when used as sensory additives in feed for all animal species.
EFSA journal. European Food Safety Authority    June 12, 2020   Volume 18, Issue 6 e06146 doi: 10.2903/j.efsa.2020.6146
Bampidis V, Azimonti G, Bastos ML, Christensen H, Kos Durjava M, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A, Petkova M....Following a request from the European Commission, the EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on the safety and efficacy of turmeric extract, turmeric oil, turmeric oleoresin and turmeric tincture from L. rhizome when used as sensory additives in feed and in water for drinking for all animal species. The FEEDAP Panel concludes that the additives under consideration are safe at the maximum proposed use levels: (i) turmeric extract at 15 mg/kg complete feed (or in water for drinking at comparable exposure) for a...
Donkey Feeding During Maintenance, Pregnancy, and Lactation: Effects on Body Weight, Milk Production, and Foal Growth.
Journal of equine veterinary science    May 22, 2020   Volume 91 103131 doi: 10.1016/j.jevs.2020.103131
Salari F, Licitra R, Altomonte I, Martini M.We evaluated the daily intake in donkeys during maintenance, late pregnancy, and early lactation. The growth curves of the foals in the first eight weeks of life and the milk production in lactating jennies were also investigated. Donkeys were separated into two groups: seven pregnant jennies (group 1: pregnant/lactating) and seven nonpregnant, nonlactating jennies (group 2). The groups were fed two different diets each. The feeding period for group 1 covered eight weeks before parturition and eight weeks postpartum. Group 2 was managed during the same time period (16 weeks). Diet 1 consisted...
The effect of crude protein content of the diet on renal energy losses in horses.
Journal of animal physiology and animal nutrition    May 14, 2020   Volume 104, Issue 5 1494-1500 doi: 10.1111/jpn.13377
Kuchler M, Zeyner A, Susenbeth A, Kienzle E.Renal energy losses of horses are high in comparison with other species. In the present study, more data were obtained on this parameter to improve predictive equations for renal energy losses. Four adult ponies (247-344 kg body weight [BW]) were fed with eight different diets based on first cut hay, second cut hay, early first cut fresh grass, late cut herbs-grass mix, early cut clover-grass mix, sugar beet pulp, rice bran and straw. Feed intake was measured, and urine and faeces were quantitatively collected for 3 × 12 hr at daytime and afterwards 3 × 12 hr at nighttime. Feed was an...
The Influence of Topically Applied Oil-Based Palatants on Eating Behavior in Horses.
Journal of equine veterinary science    April 9, 2020   Volume 91 102995 doi: 10.1016/j.jevs.2020.102995
Francis JM, Neander CR, Roeder MJ, Perry EB.Palatants may be added to equine feed and medication either during or after manufacturing to enhance product acceptance. Prior studies have examined a variety of palatants but results have been limited and inconsistent. Therefore, the objective of this research was to evaluate topically applied oil-based palatants on feeding preferences in horses. Stock-type horses (n = 10) were used in this paired preference test across a two-phase study. Phase one compared six palatants (banana, anise, peppermint, apple, spearmint, and orange) to a control (corn oil), whereas phase two compared preference a...
Investigation of plasma concentrations of paracetamol, metacetamol, and orthocetamol in Japanese racehorses using liquid chromatography-electrospray ionisation-tandem mass spectrometry.
Drug testing and analysis    April 1, 2020   Volume 12, Issue 7 929-937 doi: 10.1002/dta.2792
Ishii H, Obara T, Kijima-Suda I.Paracetamol is used widely as an over-the-counter analgesic and antipyretic medication for humans, but not for Japanese racehorses. Paracetamol can be an environmental substance, and is found together with its two isomers, metacetamol and orthocetamol, in equine urine. However, the sources and routes of paracetamol exposure remain unclear. To control the misuse of paracetamol, it is appropriate to establish residue limits for paracetamol to differentiate the administration of paracetamol from its environmental levels. In this study, we developed and validated a quantitative method for paraceta...
Effect of feed deprivation on daily water consumption in healthy horses.
Equine veterinary journal    March 30, 2020   Volume 53, Issue 1 117-124 doi: 10.1111/evj.13259
Freeman DE, Mooney A, Giguère S, Claire J, Evetts C, Diskant P.Measurements of water consumed by fed healthy horses might not apply to horses that are unwilling or unable to drink or are not fed for any reason. Objective: To examine the effects of feed deprivation on voluntary water consumption compared with fed conditions. Methods: In vivo experiment. Methods: Eight healthy adult Thoroughbred geldings were used in a randomised crossover design so that each horse served as its own control for fed vs feed-deprived conditions. Water intake, bodyweight, physical findings and vital signs were measured during 4 days of feeding and 4 days of feed deprivation....