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Topic:Horses

"Horses" is a broad topic that encompasses various aspects of equine biology, behavior, and management. This category includes studies on the anatomy, physiology, and genetics of horses, as well as their behavior, nutrition, and care. Research in this area may also cover the historical and cultural significance of horses, their roles in agriculture, sport, and therapy, and the challenges associated with their conservation and welfare. The page aggregates peer-reviewed research articles and scholarly studies that explore the multifaceted relationships between humans and horses, examining both scientific and socio-economic perspectives.
A new genomic tool, ultra-frequently cleaving TaqII/sinefungin endonuclease with a combined 2.9-bp recognition site, applied to the construction of horse DNA libraries.
BMC genomics    June 1, 2013   Volume 14 370 doi: 10.1186/1471-2164-14-370
Zylicz-Stachula A, Zolnierkiewicz O, Jasiecki J, Skowron PM.Genomics and metagenomics are currently leading research areas, with DNA sequences accumulating at an exponential rate. Although enormous advances in DNA sequencing technologies are taking place, progress is frequently limited by factors such as genomic contig assembly and generation of representative libraries. A number of DNA fragmentation methods, such as hydrodynamic sharing, sonication or DNase I fragmentation, have various drawbacks, including DNA damage, poor fragmentation control, irreproducibility and non-overlapping DNA segment representation. Improvements in these limited DNA scissi...
Y-chromosome analysis in Retuertas horses.
PloS one    May 31, 2013   Volume 8, Issue 5 e64985 doi: 10.1371/journal.pone.0064985
Brandariz-Fontes C, Leonard JA, Vega-Pla JL, Backström N, Lindgren G, Lippold S, Rico C.Several studies based on a variety of genetic markers have attempted to establish the origins of horse domestication. Thus far a discrepancy between the results of mitochondrial DNA analysis, which show high levels of diversity, and results from the Y-chromosome, with almost no genetic variability, has been identified. Most previous work on the horse Y-chromosome has focused on widespread, popular breeds or local Asian breeds. It is possible that these breeds represent a reduced set of the genetic variation present in the species. Additional genetic variation may be present in local breeds and...
Accelerometric comparison of the locomotor pattern of horses sedated with xylazine hydrochloride, detomidine hydrochloride, or romifidine hydrochloride.
American journal of veterinary research    May 31, 2013   Volume 74, Issue 6 828-834 doi: 10.2460/ajvr.74.6.828
López-Sanromán FJ, Holmbak-Petersen R, Varela M, del Alamo AM, Santiago I.To evaluate the duration of effects on movement patterns of horses after sedation with equipotent doses of xylazine hydrochloride, detomidine hydrochloride, or romifidine hydrochloride and determine whether accelerometry can be used to quantify differences among drug treatments. Methods: 6 healthy horses. Methods: Each horse was injected IV with saline (0.9% NaCl) solution (10 mL), xylazine diluted in saline solution (0.5 mg/kg), detomidine diluted in saline solution (0.01 mg/kg), or romifidine diluted in saline solution (0.04 mg/kg) in random order. A triaxial accelerometric device was used f...
Effect of gluteus medius muscle sample collection depth on postprandial mammalian target of rapamycin signaling in mature Thoroughbred mares.
American journal of veterinary research    May 31, 2013   Volume 74, Issue 6 910-917 doi: 10.2460/ajvr.74.6.910
Wagner AL, Urschel KL, Lefta M, Esser KA.To determine the effect of biopsy collection depth on the postprandial activation of mammalian target of rapamycin (mTOR) signaling factors, particularly protein kinase B, ribosomal protein S6 kinase, ribosomal protein S6, and eukaryotic initiation factor 4E binding protein 1 in middle-aged horses. Methods: 6 healthy Thoroughbred mares (mean ± SD age, 13.4 ± 3.4 years). Methods: Horses were fed a high-protein feed at 3 g/kg. Sixty minutes after horses were fed, the percutaneous needle biopsy technique was used to collect biopsy specimens from the gluteus medius muscle at 6, 8, and 10 cm belo...
Clinical snapshot: depression and hemorrhagic diarrhea in a thoroughbred colt.
Compendium (Yardley, PA)    May 31, 2013   Volume 34, Issue 6 E4 
Close K.No abstract available
Performance of a gravitational marrow separator, multidirectional bone marrow aspiration needle, and repeated bone marrow collections on the production of concentrated bone marrow and separation of mesenchymal stem cells in horses.
American journal of veterinary research    May 31, 2013   Volume 74, Issue 6 854-863 doi: 10.2460/ajvr.74.6.854
Ishihara A, Helbig HJ, Sanchez-Hodge RB, Wellman ML, Landrigan MD, Bertone AL.Objective-To determine the efficiency of a novel point-of-care gravitational marrow separator and bone marrow aspiration needle for concentrated bone marrow production and bone marrow-derived mesenchymal stem cell (MSC) separation and assess the effect of repeated bone marrow collections in horses. Animals-8 healthy adult horses. Procedures-Bone marrow aspiration was performed twice (1 month apart) from sternebral bodies with a standard or prototype multidirectional needle. Concentrated bone marrow was obtained by gravitational marrow separation and evaluated for WBC and platelet counts, autom...
Telemetric analysis of breathing pattern variability in recurrent airway obstruction (heaves)-affected horses.
American journal of veterinary research    May 31, 2013   Volume 74, Issue 6 925-933 doi: 10.2460/ajvr.74.6.925
Behan AL, Hauptman JG, Robinson NE.To use noninvasive respiratory inductance plethysmography (RIP) to investigate differences in breathing patterns between horses with and without recurrent airway obstruction (RAO) during the onset of airway obstruction induced through confinement to stables. Methods: 12 horses with no history or clinical signs of respiratory disease (control horses) and 7 RAO-affected horses. Methods: The study involved 2 phases. In phase 1, the optimal position of RIP bands for recording pulmonary function was investigated in 12 control horses. In phase 2, 7 RAO-affected and 7 control horses were confined to ...
Evaluation of osteochondral sample collection guided by computed tomography and magnetic resonance imaging for early detection of osteoarthritis in centrodistal joints of young Icelandic horses.
American journal of veterinary research    May 31, 2013   Volume 74, Issue 6 874-887 doi: 10.2460/ajvr.74.6.874
Ley CJ, Ekman S, Dahlberg LE, Björnsdóttir S, Hansson K.To evaluate the use of CT and MRI for guidance of osteochondral sample collection for histologic detection of early osteoarthritic lesions in centrodistal (distal intertarsal) joints of horses. Methods: Right tarsal joints from the cadavers of 24 Icelandic horses aged 29 to 31 months. Methods: CT and MRI were used to evaluate the extent of suspected osteoarthritic changes in centrodistal joints, which were graded with a semiquantitative system. The anatomic regions with the highest grade of change were identified, and osteochondral samples were obtained from these regions. Samples were also ob...
Clinical snapshot: Corneal edema in a Tennessee walking horse.
Compendium (Yardley, PA)    May 30, 2013   Volume 35, Issue 2 E7 
Tirosh-Levy S.No abstract available
The use of molecular and cytogenetic methods as a valuable tool in the detection of chromosomal abnormalities in horses: a case of sex chromosome chimerism in a Spanish purebred colt.
Cytogenetic and genome research    May 30, 2013   Volume 141, Issue 4 277-283 doi: 10.1159/000351225
Demyda-Peyrás S, Membrillo A, Bugno-Poniewierska M, Pawlina K, Anaya G, Moreno-Millán M.Chromosomal abnormalities associated to sex chromosomes are reported as a problem more common than believed to be in horses. Most of them remain undiagnosed due to the complexity of the horse karyotype and the lack of interest of breeders and veterinarians in this type of diagnosis. Approximately 10 years ago, the Spanish Purebred Breeders Association implemented a DNA paternity test to evaluate the pedigree of every newborn foal. All candidates who showed abnormal or uncertain results are routinely submitted to cytogenetical analysis to evaluate the presence of chromosomal abnormalities. We s...
Assessment of the wind-up phenomenon in the equine nociceptive trigeminal system.
Veterinary journal (London, England : 1997)    May 30, 2013   Volume 198, Issue 1 81-87 doi: 10.1016/j.tvjl.2013.04.012
Veres-Nyéki KO, Leandri M, Spadavecchia C.Repeated sub-threshold nociceptive electrical stimulation resulting in temporal summation of the limb nociceptive withdrawal reflex is a well-established non-invasive model to investigate the wind-up phenomenon in horses. Due to structural similarities of the trigeminal sensory nucleus to the dorsal horn of the spinal cord, temporal summation should be evoked by repeated transcutaneous electrical stimulation of trigeminal afferents. To evaluate this hypothesis repeated transcutaneous electrical stimulation was applied to the supraorbital and infraorbital nerves of 10 horses. Stimulation intens...
Clinical snapshot: Hirsutism in a pony.
Compendium (Yardley, PA)    May 30, 2013   Volume 35, Issue 3 E6 
Stern A.No abstract available
Comparison between standard ultrasonography, angle contrast ultrasonography, and magnetic resonance imaging characteristics of the normal equine proximal suspensory ligament. Werpy NM, Denoix JM, McIlwraith CW, Frisbie DD.Previous studies have proposed that standard ultrasonography may not adequately represent the pertinent anatomic characteristics of the equine proximal suspensory ligament. The purpose of the study was to compare the use of standard ultrasonography, angle contrast ultrasonography, MRI, and histology for identification of the anatomic characteristics of the normal equine suspensory ligament in the forelimb. Horses free from forelimb lameness with no palpable abnormalities in the region of the suspensory ligament were included in the study. The proximal suspensory ligaments in 20 forelimbs were ...
Prolonged anesthesia using sevoflurane, remifentanil and dexmedetomidine in a horse.
Veterinary anaesthesia and analgesia    May 29, 2013   Volume 40, Issue 5 521-526 doi: 10.1111/vaa.12048
Benmansour P, Duke-Novakovski T.A 10-year old Arabian mare had a slow-growing mass on the lower right mandible and required a large partial mandibulectomy. Methods: No abnormalities were detected apart from the mass. Results: A temporary tracheostomy was performed pre-operatively. Anesthesia was induced with xylazine followed by ketamine and diazepam. For 13 hours, anesthesia was maintained using sevoflurane, dexmedetomidine and remifentanil infusions, with the exception of surgical preparation time. Intra-operatively, ventilation was delivered through the cuffed tracheotomy tube. Heart and respiratory rates, ECG, arterial ...
Development of an enzyme-linked immunosorbent assay using a recombinant LigA fragment comprising repeat domains 4 to 7.5 as an antigen for diagnosis of equine leptospirosis.
Clinical and vaccine immunology : CVI    May 29, 2013   Volume 20, Issue 8 1143-1149 doi: 10.1128/CVI.00245-13
Yan W, Saleem MH, McDonough P, McDonough SP, Divers TJ, Chang YF.Leptospira immunoglobulin (Ig)-like (Lig) proteins are a novel family of surface-associated proteins in which the N-terminal 630 amino acids are conserved. In this study, we truncated the LigA conserved region into 7 fragments comprising the 1st to 3rd (LigACon1-3), 4th to 7.5th (LigACon4-7.5), 4th (LigACon4), 4.5th to 5.5th (LigACon4.5-5.5), 5.5th to 6.5th (LigACon5.5-6.5), 4th to 5th (LigACon4-5), and 6th to 7.5th (LigACon6-7.5) repeat domains. All 7 recombinant Lig proteins were screened using a slot-shaped dot blot assay for the diagnosis of equine leptospirosis. Our results showed that Li...
Anatomical location and culture of equine corneal epithelial stem cells.
Veterinary ophthalmology    May 28, 2013   Volume 17, Issue 2 106-112 doi: 10.1111/vop.12050
Moriyama H, Kasashima Y, Kuwano A, Wada S.To identify morphologically the locations of equine corneal epithelial stem cells (CESCs) and to culture these cells. Methods: We studied the eyes of 12 adult thoroughbred horses. Methods: Eye tissues were immunostained for two positive stem cell markers (p63, CK14) and one negative marker (CK3) to identify the locations of CESCs, so we could compare their immunostaining patterns with those of human stem cells previously reported. We compared the proliferation rates and morphological features of epithelial cells isolated from the corneal limbus and central cornea. Results: Undifferentiated cel...
Lethargy, fever, and anorexia in a thoroughbred weanling.
Compendium (Yardley, PA)    May 28, 2013   Volume 34, Issue 4 E1 
Page A.No abstract available
Management of zebras and zebra hybrids (zebroids).
Compendium (Yardley, PA)    May 28, 2013   Volume 34, Issue 9 E4 
Wiedner EB, Lindsay WA, Isaza R.Equine practitioners are sometimes asked to treat zebras or zebra-horse or zebra-donkey hybrids. Although these equids are subject to many of the same health issues as domestic horses, they cannot be handled like horses and generally require heavy sedation to full anesthesia, even for minor procedures. This usually necessitates the use of ultrapotent narcotics administered by remote delivery systems. This article discusses the handling, sedation, anesthesia, and common medical issues of zebras and zebra hybrids.
Standing open-flank approach for removal of enlarged pathologic ovaries in mares.
The Veterinary record    May 28, 2013   Volume 172, Issue 26 687 doi: 10.1136/vr.101380
Kelmer G, Raz T, Berlin D, Steinman A, Tatz AJ.No abstract available
Advanced imaging in equine dental disease.
The Veterinary clinics of North America. Equine practice    May 28, 2013   Volume 29, Issue 2 397-vi doi: 10.1016/j.cveq.2013.04.009
Selberg K, Easley JT.Dental and sinus disorders are relatively common and of major clinical importance in equine medicine. Advanced diagnostic imaging has become an integral part of equine veterinary medicine. Advanced imaging has progressed the understanding, diagnosis, and treatment of dental- and sinus-related diseases. As a clinician, it is important to realize the value of advanced diagnostic imaging. Although computed tomography and magnetic resonance imaging are both significantly more expensive compared with other diagnostic tools, the financial cost of inaccurate diagnosis and treatment can often result i...
Region-specific expression of aquaporin subtypes in equine testis, epididymis, and ductus deferens.
Anatomical record (Hoboken, N.J. : 2007)    May 27, 2013   Volume 296, Issue 7 1115-1126 doi: 10.1002/ar.22709
Klein C, Troedsson MH, Rutllant J.The process of water movement in the excurrent duct system of the male reproductive tract is pivotal for establishment of male fertility. The objective was to elucidate expression of aquaporin (AQP) water channels in the stallion reproductive tract. Real-time RT-PCR detected expression of AQP0-5 and AQP7-11 in testis, epididymis, and ductus deferens of mature stallions. There were two main expression patterns: (1) higher expression in testis than in epididymis and ductus deferens (AQP0, -4, -5, -8, -10, and -11); and (2) lower expression in testis than in epididymis and ductus deferens (AQP1, ...
Pharmacokinetics, pharmacodynamics, metabolism, toxicology and residues of phenylbutazone in humans and horses.
Veterinary journal (London, England : 1997)    May 27, 2013   Volume 196, Issue 3 294-303 doi: 10.1016/j.tvjl.2013.04.019
Lees P, Toutain PL.The presence of horse meat in food products destined for human consumption and labelled as beef has raised several concerns of public interest. This review deals solely with one aspect of these concerns; samples of equine tissue from horses destined for the human food chain have tested positive for the non-steroidal anti-inflammatory drug, phenylbutazone. The safety of some or all such foods for human consumers is a major concern, because it was shown many years ago that phenylbutazone therapy in humans can be associated with life threatening blood dyscrasias. As an initial basis for assessing...
Resonance assignments for latherin, a natural surfactant protein from horse sweat.
Biomolecular NMR assignments    May 26, 2013   Volume 8, Issue 1 213-216 doi: 10.1007/s12104-013-9485-3
Vance SJ, McDonald RE, Cooper A, Kennedy MW, Smith BO.Latherin is an intrinsically surfactant protein of ~23 kDa found in the sweat and saliva of horses. Its function is probably to enhance the translocation of sweat water from the skin to the surface of the pelt for evaporative cooling. Its role in saliva may be to enhance the wetting, softening and maceration of the dry, fibrous food for which equines are adapted. Latherin is unusual in its relatively high content of aliphatic amino acids (~25% leucines) that might contribute to its surfactant properties. Latherin is related to the palate, lung, and nasal epithelium carcinoma-associated protein...
Identification of immuno-dominant antigens of Trypanosoma evansi for detection of chronic trypanosomosis using experimentally infected equines.
Research in veterinary science    May 25, 2013   Volume 95, Issue 2 522-528 doi: 10.1016/j.rvsc.2013.04.030
Yadav SC, Kumar R, Kumar V, Jaideep , Kumar R, Gupta AK, Bera BC, Tatu U.Trypanosoma evansi is the most extensively distributed trypanosome responsible for disease called surra in livestock in many countries including frequent outbreaks in India. The prevalence of this disease is most commonly reported by standard parasitological detection methods (SPDM); however, antibody ELISA is being in practice by locally produced whole cell lysate (WCL) antigens in many countries. In the present investigation, we attempted to identify and purify immuno dominant, infection specific trypanosome antigens from T. evansi proteome using experimentally infected equine serum by immun...
Outbreak of upper respiratory disease in horses caused by Streptococcus equi subsp. zooepidemicus ST-24.
Veterinary microbiology    May 25, 2013   Volume 166, Issue 1-2 281-285 doi: 10.1016/j.vetmic.2013.05.006
Lindahl SB, Aspán A, Båverud V, Paillot R, Pringle J, Rash NL, Söderlund R, Waller AS.Streptococcus equi subsp. zooepidemicus (S. zooepidemicus) is generally considered a commensal and an opportunistic pathogen of the upper airways in horses. Establishing whether certain strains of S. zooepidemicus can cause upper respiratory disease as a host-specific pathogen of horses, and if there are certain genogroups of S. zooepidemicus that are more virulent than others is of major clinical importance. In this study, we describe an outbreak of upper respiratory disease in horses that was associated with S. zooepidemicus. Upper respiratory samples were cultured, analyzed by real-time PCR...
Practical application of acceleromyography to monitor neuromuscular block in a horse.
Veterinary anaesthesia and analgesia    May 25, 2013   Volume 40, Issue 5 554-556 doi: 10.1111/vaa.12049
Marly C, Gent T, Mosing M.No abstract available.
Genetic analysis of haematological and plasma biochemical parameters in the Spanish purebred horse exercised on a treadmill.
Animal : an international journal of animal bioscience    May 24, 2013   Volume 7, Issue 9 1414-1422 doi: 10.1017/S1751731113000955
Escribano BM, Molina A, Valera M, Tovar P, Agüera EI, Santisteban R, Vivo R, Agüera S, Rubio MD.The novel aim of this study was to describe the reference values of different haematological and biochemical parameters in the Spanish purebred horse (Andalusian, SPB) in each of the stages of a programmed exercise on a treadmill system, and to establish heritability and genetic correlations for these haematological and biochemical parameters. For this, 94 young SPB male horses (4.22 ± 2.27 years old) were used. An increasing intensity exercise test at 4, 5, 6 and 7 m/s was carried out on a treadmill (6% inclination). Total red blood cells, total white blood cells, neutrophils and lymphocytes...
Development of loop-mediated isothermal amplification (LAMP) for detection of Theileria equi.
Acta tropica    May 24, 2013   Volume 127, Issue 3 245-250 doi: 10.1016/j.actatropica.2013.05.007
Xie J, Liu G, Tian Z, Luo J.Several approaches have been developed for diagnosis of Theileria equi infection in horses and donkeys but all of them have limitations in practice. Due to numerous strengths including easy operation, cheapness and high sensitivity and specificity, LAMP has been already extensively used for surveillance of a number of diseases. We here set up a LAMP assay based on 18S rRNA gene for T. equi diagnosis. The approach was specific enough to differentiate T. equi from other evolutionary-related protozoa. Moreover, it was sensitive enough that LAMP was capable of detecting as much low as 10 copy targ...
Descriptive epidemiology of African horse sickness in Zimbabwe.
The Onderstepoort journal of veterinary research    May 24, 2013   Volume 80, Issue 1 578 doi: 10.4102/ojvr.v80i1.578
Gordon S, Bolwell C, Rogers C, Guthrie A, Magunda F, Hove P.A study of the prevalence of African horse sickness in horses was conducted, using records from two private equine practices in Harare for the period 1998-2004. Results indicated a higher prevalence of the disease in horses in Zimbabwe in the late rainy season (March - May). Age of the horse was found to be a significant risk factor, with foals or yearlings appearing to be 1.80 times more likely to contract the disease compared with horses older than two years. The case fatality rate in foals or yearlings was also higher than in older age groups, but this difference was not significant. The va...
Results of upper airway radiography and ultrasonography predict dynamic laryngeal collapse in affected horses.
Equine veterinary journal    May 23, 2013   Volume 45, Issue 6 705-710 doi: 10.1111/evj.12066
Fjordbakk CT, Chalmers HJ, Holcombe SJ, Strand E.The pathogenesis of dynamic bilateral laryngeal collapse (DLC) associated with poll flexion is unknown. Diagnosis is dependent upon exercise endoscopy while replicating the flexed head position harness racehorses experience during racing. Objective: To describe the effects of poll flexion on rostrocaudal laryngeal positioning and laryngeal lumen width in resting horses diagnosed with DLC compared to controls, and to establish diagnostic criteria for DLC by use of diagnostic imaging. Methods: Case-control study. Methods: Fifty harness racehorses were prospectively included in the study: 25 case...