Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Kania M, Mikołajewska D, Marycz K, Kobielarz M.The aim of this research was to assess the effect of diet supplementation with zinc and copper, in different chemical forms (organic and inorganic), on the mechanical properties of the hair of healthy English thoroughbred horses. Hairs were taken from 18 horses which had been fed with oats and hay for a period of 110 days. Twelve of the horses had been additionally given a daily dose of 700 g of highquality 44-ingredients Fohlengold St-Hippolyt muesli made by Muhle Ebert Dilheim. Six of them had received the muesli-containing organic zinc and copper (OS), while the other six horses had receive...
Haridy FM, Shoukry NM, Hassan AA, Morsy TA.Toxoplasma gondii is one of the important zoonotic parasites of worldwide. In this paper the seroprevalence of T. gondii in draught horses (3-15 years) including 90 males and 10 females in the first half of the year 2009 was studied. The result showed that the overall ELISA-T. gondii antibodies were 25% of the horses in Greater Cairo, 50% (females) and 22.2% (males).
Hirota K, Kakoi H, Gawahara H, Hasegawa T, Tozaki T.We characterized the SNP 53 JPN System for parentage verification during horse registry. The SNP 53 JPN System was constructed using 53 highly polymorphic single nucleotide polymorphisms (SNPs), which were amplified and genotyped with 2 multiplex assays. The SNP 53 JPN System showed good resolution for 95 unrelated thoroughbreds, and the exclusion probability (PE01) for each SNP ranged from 11.5 to 23.0%, resulting in a total PE01 value of 99.996%. These results indicate that the SNP 53 JPN System is useful for parentage testing of thoroughbreds. Of the 53 SNPs, 8 SNPs could be used to exclude...
Field H, Schaaf K, Kung N, Simon C, Waltisbuhl D, Hobert H, Moore F, Middleton D, Crook A, Smith G, Daniels P, Glanville R, Lovell D.To determine the epidemiologic and clinical features of a 2008 outbreak of Hendra virus infection in a veterinary clinic in Australia, we investigated the equine case-series. Four of 5 infected horses died, as did 1 of 2 infected staff members. Clinical manifestation in horses was predominantly neurologic. Preclinical transmission appears likely.
Stancampiano L, Mughini Gras L, Poglayen G.The spatial distribution of large intestinal helminth parasites of 50 horses was studied. Both adult and mucosal larval stages were examined and counted within the ventral colon, dorsal colon and caecum. Thirty-three species of adult parasites were identified and their distribution in the three large intestinal regions reported, together with the localization of cyathostome encysted larvae and of empty mucosal cysts. In order to highlight interspecific interactions, both Spearman pairwise correlation (among all parasites) and multivariable negative binomial regression (among the most abundant ...
De Martino L, Lucido M, Mallardo K, Facello B, Mallardo M, Iovane G, Pagnini U, Tufano MA, Catalanotti P.Methicillin-resistant staphylococci (MRS) were isolated from nasal swabs of 56 of 159 (35.2%; 95% confidence interval [CI]: 27.9-43.2%) healthy horses. Two nasal swabs were collected from each horse; 43 of 159 (27%; 95% CI: 20.5-34.8%) of the cohort were colonized by MRS strains in 1 nostril, while in the remaining 13 of 159 (8.2%; 95% CI: 4.6-13.9%), different or identical MRS strains were isolated in both nostrils. Of the 29 humans in close contact with the horses tested, 4 (13.8%; 95% CI: 4.5-32.6%) were found to be carriers of MRS. All isolates were coagulase negative with the exception of...
Kouam MK, Kantzoura V, Gajadhar AA, Theis JH, Papadopoulos E, Theodoropoulos G.Serum samples were collected from a total of 544 equids that included 524 horses, 13 mules, and 7 ponies from various regions of mainland Greece and were examined by competitive-inhibition ELISA (cELISA) to evaluate the level of exposure of Greek equids to Theileria (Babesia) equi and/or Babesia caballi, the causative agents of piroplasmosis. Association between seropositivity and host-related factors of species, gender, age, origin, activity and location were investigated. The overall seroprevalence was 11.6% (9.1-14.6%) with 95% confidence limit. The seroprevalence for T. equi and B. caballi...
Hill EW, Gu J, Eivers SS, Fonseca RG, McGivney BA, Govindarajan P, Orr N, Katz LM, MacHugh DE.Variants of the MSTN gene encoding myostatin are associated with muscle hypertrophy phenotypes in a range of mammalian species, most notably cattle, dogs, mice, and humans. Using a sample of registered Thoroughbred horses (n = 148), we have identified a novel MSTN sequence polymorphism that is strongly associated (g.66493737C>T, P = 4.85x10(-8)) with best race distance among elite racehorses (n = 79). This observation was independently validated (P = 1.91x10(-6)) in a resampled group of Thoroughbreds (n = 62) and in a cohort of Thoroughbreds (n = 37, P = 0.0047) produced by the same trainer...
Kwok WH, Leung DK, Leung GN, Wan TS, Wong CH, Wong JK.A rapid liquid chromatography-tandem mass spectrometry (LC-MS-MS) method was developed for the simultaneous screening of 19 drugs of different classes in equine plasma using automated on-line solid-phase extraction (SPE) coupled with a triple quadrupole mass spectrometer. Plasma samples were first protein precipitated using acetonitrile. After centrifugation, the supernatant was directly injected into the on-line SPE system and analysed by a triple quadrupole LC-MS-MS in positive electrospray ionisation (+ESI) mode with selected reaction monitoring (SRM) scan function. On-line extraction and c...
Loeffler A, Lloyd DH.This article reviews the literature on the epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in dogs, cats and horses. Over the past 10 years, MRSA has emerged as an important pathogen in veterinary medicine, especially in countries with a high MRSA burden in human hospitals. During the same period, community-associated MRSA (CA-MRSA) infections in humans without apparent links to healthcare facilities have increased dramatically. Although animal infections occur outside human hospitals, significant epidemiological, clinical and genetic differences exist between CA-MRSA in hum...
Martínez B, Celda MF, Anastasio B, García I, López-Mendoza MC.Fifty-five bovine, 50 equine, 60 ovine, and 50 porcine carcasses were sampled in a slaughterhouse in eastern Spain. Two samples were taken from each carcass, one using the excision method and the other using the swabbing method. Four different materials were used for swabbing: cellulose, polyurethane, or viscose sponges, and medical gauze. Samples were collected at the end of the process by four different people before the carcasses were taken to the cooler. The samples were examined for total viable bacteria counts (TVCs) and Enterobacteriaceae counts (ECs). The mean TVC for all species sampl...
Huby-Chilton F, Murphy J, Chilton NB, Gajadhar AA, Blais BW.Single-strand conformation polymorphism (SSCP) analysis of amplicons produced from a mitochondrial DNA region between the tRNA(Lys) and ATPase8 genes was applied for the detection of animal product within livestock feeds. Identification of prohibited animal (cattle, elk, sheep, deer, and goat) and nonprohibited animal (pig and horse) products from North America was possible based on the differential display of the single-stranded DNA fragments for the different animal species on SSCP gels. This method allowed specific detection and identification of mixed genomic DNA from different animal spec...
Walter I, Tschulenk W, Budik S, Aurich C.The present study gives a detailed ultrastructural description of equine conceptuses at Day 14 (n = 2) and Day 16 (n = 3) after ovulation. Whereas on Day 14 only primitive structures were seen, on Day 16 neurulation and formation of mesodermal somites had taken place. The ectoderm of the embryo itself and the surrounding trophoblast ectodermal cells were characterised by specific cell surface differentiations. At the embryonic ectodermal cell surface (14 and 16 days) remarkable protruded and fused cytoplasmic projections were seen, typically associated with macropinocytotic events involved in ...
Virmani N, Bera BC, Singh BK, Shanmugasundaram K, Gulati BR, Barua S, Vaid RK, Gupta AK, Singh RK.An outbreak of equine influenza (EI) was reported in India in June, 2008 after a gap of two decades. The outbreak started from Jammu and Kashmir (Katra), northern state of India and spread to the other parts of the country affecting equines in 11 states. The virus (H3N8) was isolated from nasal swabs obtained from clinical cases in various locations in the country including Katra (Jammu and Kashmir), Mysore (Karnataka) and Ahmedabad (Gujarat) using embryonated chicken eggs. The virus isolates were identified as H3N8 by haemagglutination inhibition (HI) test titration with standard serum and by...
DeAssis JB, DeLaat DM, Peixoto MG, Bergmann JA, Fonseca CG, Carvalho MR.One hundred and fifteen unrelated Mangalarga Marchador horses were sampled from three geographically distinct regions of Minas Gerais State, Brazil (South, Southeast, and Northeast) and tested for 10 microsatellite loci. Genetic diversity and population structure parameters were estimated with ARLEQUIN 3.0, CERVUS 2.0, POPGENE 1.31, GENEPOP on the web, STRUCTURE 2.0, and SPAGEDI 1.2 software packages. Under Hardy-Weinberg assumptions, seven markers were at equilibrium (LEX014, LEX017, LEX019, SGCV23, TKY321, VHL20, and VIASH39), while two (ASB3 and LEX031) presented significant homozygote exce...
Payne RJ, Lean MS, Greet TR.Between October 2000 and January 2007, 24 horses were presented with suspected squamous cell carcinoma (SCC) of the third eyelid. The hospital's medical records were analysed retrospectively to gain data about the cases, and telephone follow-up was obtained from the owners and referring veterinary surgeons. The resected third eyelid was submitted for histological examination in 21 cases; in the other three cases the tissue was not submitted at the owners' request, for economic reasons. SCC was confirmed in 16 of these 21 cases, three cases were diagnosed histologically as lymphoid hyperplasia,...
Acosta-Jamett G, Cleaveland S, Cunningham AA, Bronsvoort BM, Craig PS.Cyst echinococcosis (CE) is one of the most important zoonosis in Chile, where studies have focussed mainly in moist southern regions. The present study was conducted to determine the prevalence of cystic echinococcosis (CE) in livestock and humans in the semiarid Coquimbo region in north-central Chile. A review of all surgical cases of CE in humans reported in the Elqui, Limarí and Choapa provinces in Coquimbo region for the period comprising 1995-2006 was obtained. In addition, a retrospective study of CE covering condemnation records from slaughterhouses of these provinces from the same pe...
Robinson E, Carmalt JL.To report inguinal herniation of the ascending colon in a Standardbred colt. Methods: Case report. Methods: A 6-month-old Standardbred colt. Methods: The colt underwent surgical exploration of the hernia with large colon resection and anastomosis. Results: A successful large colon resection and anastomosis was completed, however, the colt was euthanatized at the end of surgery upon owner request. Conclusions: Although uncommon, indirect inguinal herniation of the ascending colon can occur in young horses.
Houghton E, Maynard S.This chapter reviews drug and medication control in equestrian sports and addresses the rules of racing, the technological advances that have been made in drug detection and the importance of metabolism studies in the development of effective drug surveillance programmes. Typical approaches to screening and confirmatory analysis are discussed, as are the quality processes that underpin these procedures. The chapter also addresses four specific topics relevant to equestrian sports: substances controlled by threshold values, the approach adopted recently by European racing authorities to control...
Smith AJ, Felstead CW, Lawson JS, Weller R.Radiography is the most commonly applied imaging modality in equine practice and forms an essential part of the diagnostic work-up of lame horses. Radiographic signs of musculoskeletal pathology are frequently localized at sites of soft tissue attachment, which are often not clearly visible on radiographs. Different lesions carry different prognoses and require a variety of treatments, and a good knowledge of the position of the synovial structures in the distal limb of the horse is essential for practitioners in the interpretation of radiographs. This study describes a new technique for creat...
Frisbie DD, Lu Y, Kawcak CE, DiCarlo EF, Binette F, McIlwraith CW.Current autologous chondrocyte implantation (ACI) techniques require 2 surgical procedures: 1 for cell harvest and 1 for reimplantation of cultured cells. A 1-step procedure is more desirable. Objective: A 1-step surgical procedure using autologous cartilage fragments on a polydioxanone scaffold, or CAIS (cartilage autograft implantation system), in a clinically relevant defect (15-mm diameter) within equine femoral trochlea was compared with a 2-step ACI technique as well as with empty defects and defects with polydioxanone foam scaffolds alone. Methods: Controlled laboratory study. Methods: ...
Severa L, Máchal L, Svábová L, Mamica O.This study quantified and evaluated the variability of sperm head shape for 10 different stallions. Sperm head shape characteristics including sperm head length to width ratio, position of the center of gravity, curvature, and degree of roundness were assessed and analysed from images using elliptic Fourier descriptors and inverse Fourier transformation. The first four principal components accounted for 88.46-92.33% of the total variance and provided a good summary of the overall data. In the case of the ejaculate with defective sperm heads the components accounted for 97.35-98.21% of variatio...
Castagnetti C, Pirrone A, Mariella J, Mari G.The use of blood lactate concentration as an indicator of prognosis and disease severity has become a common practice in equine medicine, especially with the validation of handheld analyzers. However, few authors described lactate concentration in critically ill foals, and there are no published studies about the use of handheld analyzers in neonatal foals. In this study, for the first time in the equine neonate, we validated the Lactate Scout analyzer, both in healthy and in critically ill foals. The study also describes the normal range for blood lactate in 26 healthy neonatal foals during t...
Jelinski MD, Campbell JR.The objective of this study was to determine the number of hours veterinarians in western Canada work per week, how they apportion their time by species, and clinics' hiring intentions for new veterinary associates. Of 1099 clinics contacted, 706 (64%) responded to the survey, representing 80% (1774/2227) of private practitioners in western Canada. Practitioners devoted 73% of their time to small animals (SA), 11% to beef practice, and 9% to horses. Sixty-four percent of clinics and 66% of practitioners were devoted exclusively to companion animal (SA and horses) practice; only 4% of clinics a...
Magnus RP.The take-home message in marketing your equine practice is simple: understand your position in the target market and the buying behavior of your current and prospective customers. Time well spent on analysis and evaluation of options can maximize customer value in the services and products you offer. This allows you to capture profit and to attain your personal and professional goals as an equine practitioner.
La Gerche A, Daffy JR, Mooney DJ, Forbes G, Davie AJ.It has been observed that microbubbles may pass through the pulmonary circulation of dogs and humans during exercise. In humans, this phenomenon has been associated with lower pulmonary artery pressures, enhanced right ventricular function and greater exercise capacity. In the exercising Thoroughbred horse, extraordinarily high cardiac outputs exert significant pulmonary vascular stresses. The aim of this study was to determine, using contrast echocardiography, whether Thoroughbred horses performing strenuous exercise developed pulmonary transit of agitated contrast microbubbles (PTAC). At res...
Olsen HF, Meuwissen T, Klemetsdal G.The aim of this study was to examine how to apply optimal contribution selection (OCS) in the Norwegian and the North-Swedish cold-blooded trotter and give practical recommendations for the future. OCS was implemented using the software Gencont with overlapping generations and selected a few, but young sires, as these turn over the generations faster and thus is less related to the mare candidates. In addition, a number of Swedish sires were selected as they were less related to the selection candidates. We concluded that implementing OCS is feasible to select sires (there is no selection on m...
Ho EN, Yiu KC, Tang FP, Dehennin L, Plou P, Bonnaire Y, Wan TS.Boldenone (1,2-dehydrotestosterone) is a common veterinary anabolic agent. Its structure is very similar to testosterone. Testosterone is endogenous in the horse, whereas there has been no report concerning the detection of endogenous boldenone. This paper reports the direct observation of sulphate conjugate of boldenone in equine urine from entires. The detection procedures involved solid-phase extraction, immunoaffinity column (IAC) purification, and then LC-MS-MS analysis on a Q-ToF instrument. The identification of boldenone sulphate has provided direct evidence for the endogenous nature o...
Calvert N, Grainger N, Hurworth M.Currently, there is a lack of cheap and effective training models to allow orthopaedic surgery trainees to learn the basics of anterior cruciate ligament reconstruction. The aim of this paper was to investigate the viability of using various animal joints as models for the training of anterior cruciate ligament reconstruction. Methods: Equine stifles, bovine stifles and carpal joints, sheep stifles and porcine stifles, were sourced from a local abattoir. Each joint was assessed for the following criteria: suitable tendons for harvesting, ease of arthroscopy access, adequacy of arthroscopy view...
Gandini M.To report a technique for closed-bowel 1-layer inverting end-to-end jejunojejunal anastomosis in horses. Methods: Experimental study. Methods: Fresh cadaveric jejunal segments from 12 horses. Methods: For each bowel segment a 1-layer closed and a 2-layer inverting end-to-end jejunojejunosotomy was created. Anastomosis construction time and anastomotic bursting pressure were measured and compared. Results: Closed-bowel anastomosis was significantly faster to create than a 2-layer technique. Luminal narrowing (<30%) was similar with both techniques and comparable with other inverting techniques....
Bayly WM, Kingston JK, Brown JA, Keegan RD, Greene SA, Sides RH.Horses experience major perturbations in acid-base balance during supramaximal exercise. Ion movement in and out of erythrocytes (RBCs) is believed to be important in maintaining acid-base balance but it is unclear as to the extent to which this happens, nor how it affects single measurements of ion concentrations in arterial and venous blood. Objective: To clarify the role RBCs play in mitigating perturbations in acid-base balance during high speed exercise in horses, and to describe associated differences in arterial (a) and mixed venous (v) concentrations of key ions. Methods: Six exercise-...
Henze A, Aumer F, Grabner A, Raila J, Schweigert FJ.Although horses and donkeys belong to the same genus, their genetic characteristics probably result in specific proteomes and post-translational modifications (PTM) of proteins. Since PTM can alter protein properties, specific PTM may contribute to species-specific characteristics. Therefore, the aim of the present study was to analyse differences in serum protein profiles of horses and donkeys as well as mules, which combine the genetic backgrounds of both species. Additionally, changes in PTM of the protein transthyretin (TTR) were analysed. Serum protein profiles of each species (five anima...
Latimer CA, Wyman M.This discussion of normal and abnormal findings on routine ophthalmic examination of the foal should prove useful to the equine practitioner who must provide counsel to the concerned owner and/or breeder. Early diagnosis and treatment of ocular abnormalities improve the prognosis for continued function and aid determination of the significance of lesions observed in later life.
Nauwelaerts S, Clayton HM.Estimates of the position of the centres of mass (CM) of body segments are usually extrapolated relative to bony landmarks as determined in cadaver studies. This extrapolation assumes that segments are rigid bodies. Since the trunk represents a large percentage of the total body mass in horses, violation of the rigid body assumption by the trunk segment has important consequences for studying the biomechanics of equine locomotion. Objective: To assess the magnitude of error in CM position due to deformability of the trunk segment and the timing of these errors during the trotting stride. The h...
Channing CP.OUR knowledge of the pathways of steroid biosynthesis in the ovary has been gained mainly by incubations of ovaries in vitro1,2. The tissues incubated have contained numerous cell types: granulosa cells, theca interna cells, stromal cells, interstitial cells, and sometimes luteal cells. Possibly such mixtures of two or more different cell types are able to secrete hormones that one cell type cannot secrete by itself3–9. Furthermore, during such incubations in vitro an exchange of precursors and products between different cell types may be facilitated because of breakdown of naturally occurri...
Bauer JE, Ransone WD.Alterations in the fatty acid distribution of total lipid extracts and 4 of the major lipid subclasses of serum in ponies fasted overnight and for 4 and 7 days were determined. Although increases in 16:0, 16:1, and 18:3 omega 3 were observed, decreased amounts of 18:0 and 18:2 omega 6 combined to cause no significant change in the saturated to unsaturated fatty acid ratio in the total extracts. Phospholipid became somewhat preferentially enriched in saturated fatty acids due to a decrease in 18:1, although this response was variable. The free fatty acid and triglyceride fractions both showed i...
Shadomy HJ, Dixon DM.An unusual fungus, probably traumatically introduced into the eye of a horse while grazing, was studied on various mycological media. Upon examination of colonies the organism produced bulbils and clearly exhibited the characteristics of a new species of Papulaspora, P. equi Shadomy & Dixon.
Szajáni B.Aminoacylase (E.C. 3.5.1.14) was isolated from the kidneys of different mammalian species (horse, cattle, rabbit and pig) by extracting the organ with water and subjecting the extract to heat treatment at 70 degrees C for 10 min, then, after having removed denatured proteins by fractionating those remaining in the solution by ammonium sulfate. The enzyme obtained in this way can either be used directly for practical purposes (e.g. preparation of immobilized aminoacylase) or further purified by chromatography. For the further purification of porcine kidney aminoacylase we applied a combination ...
Mitchell JS.Lameness and performance evaluation can be one of the most rewarding aspects of equine veterinary practice. There is a misconception that it depends on new sophisticated and expensive diagnostic modalities, when the reality is that knowing where and when to use these modalities form the real art of equine lameness practice. The most expensive ultrasound machine in the world is not very diagnostic if applied to the wrong limb. The art of lameness practice is vested in knowledge of horsemanship, an understanding of anatomy and function, and inquiring senses to sort out what the horse and his han...
Redden RF.Radiographic examination of the equine foot can provide the veterinarian and farrier with a wealth of information. Positioning and selection of exposure factors are of central importance if one is to produce radiographs of maximum diagnostic value.
Wendorff KM, Nishita T, Jabusch JR, Deutsch HF.The sequence of equine muscle carbonic anhydrase (CA-III) has been determined. The 2 reactive cysteines of the 5 such residues have been localized. A strong sequence homology to other mammalian carbonic anhydrases exists, and 91% of the residues in the equine and bovine muscle forms are identical.
Thornton JR, Lohni MD.Lactic dehydrogenase, although widely distributed in most tissues, was more highly concentrated in skeletal muscle, cardiac muscle, kidney and liver. Isoenzyme patterns showed a selective concentration of LDH5 in skeletal muscle while in the heart LDH 1 and 2 were predominant. In contrast, creatine kinase was only present in substantial concentration in skeletal and cardiac muscle. The serum concentrationof both enzymes showed a wide range of activity.
Narciso MHPM, da Luz MPF, Maia CM, Filho JNPP.Little is known about the dominance and leadership behaviors in the social structure of mules (Equus caballus × Equus asinus). Based on the frequency of threat and aggressive behaviors, we compared the social structure, dominance, and leadership of independent groups of mules and horses (n = 11 per group), and filmed the frequency of these behaviors over a period of four days. In both groups, aggressions were uncommon and, when based on threats, the social structure was linear and consistent with males ranking higher in dominance. When compared to horses, mules exhibited some agonistic so...