Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Fortier LA, Nixon AJ, Williams J, Cable CS.To isolate mesenchymal stem cells from adult horses and determine specific monolayer culture conditions required to enhance biochemically and phenotypically defined chondrocytic differentiation. Methods: 2 adult horse bone marrow donors without skeletal or hematologic abnormalities. Methods: Bone marrow was aspirated from the sternebra, and mesenchymal stem cells were isolated by centrifugation and cultured in monolayers. Subcultures were established in 24-well plates on day 13. Culture medium was harvested every 2 days, and culture of 12 of the 24 wells was terminated on day 6 and of the rema...
Park Y, Pariza MW.We analyzed fetal calf, newborn calf, horse, and adult cow sera for conjugated linoleic acid (CLA). All sera samples contained CLA, but the amounts varied. The predominant isomer was cis-9,trans-11 CLA but some samples appeared to contain substantial amounts of an isomer with the retention time of trans-10,cis-12 CLA.
Minetti AE.By refining a previously published model, a simple equation for the estimation of the mechanical internal work during locomotion is presented. The only input variables are the progression speed, the stride frequency and the duty factor, i.e. the fraction of the stride duration at which a foot is in contact with the ground. The inclusion of this last variable, easily measurable, allows to obtain a single equation for both walking and running. The model predictions have been compared with the mechanical internal work experimentally obtained on humans in several conditions: speeds (range 0.8-3.3 ...
Pearson RA, Dijkman JT, Krecek RC, Wright P.Two experiments were designed to compare the energy used in carrying loads by donkeys and ponies. In the first experiment 3 donkeys and 3 ponies were compared on treadmills in the UK. Density of load (lead shot or straw) had no significant effect on the energy cost of carrying loads; however, the energy cost of carrying a load decreased significantly (p < 0.001) as the weight of the load increased (in donkeys 6.44, 4.35 and 3.03 J/kg load/m, in ponies 5.82, 3.75 and 3.68 J/kg load/m, for loads of 13, 20 and 27 kg/100 kg liveweight (M) respectively). Differences between species were not sign...
Nasu T, Yamanaka T, Nakai M, Ogawa H.The blood supply in the equine hoof was studied by a microvascular casting corrosion technique and scanning electron microscopy in combination with observations of sections of the decalcificated digit. The dermal lamella was observed at the hoof wall and the dermal papilla at the other parts of the hoof. The microvascular architecture of the dermal lamella differed from that of dermal papilla. The vascular cast in the dermal papillar regions indicated that each papilla contained two central vessels (artery and vein), which ran parallel to each other, and the capillary plexus surrounding these ...
Slebodziński AB, Brzezińska-Slebodzińska E, Nowak J, Kowalska K.It is generally accepted that hormones and tissue growth factors are supplied from mother to neonate via mammary secretion. Among the protein hormones, insulin and prolactin are considered as the most important milk components for neonates. The significance of the thyroid hormones, namely triiodothyronine (T3) generated locally by 5'-monodeiodinase (5'-MD) in the mammary tissues, for the mammary gland itself and for suckling neonates is still under consideration. In the present study the activity of the 5'-MD and the concentrations of T3 and insulin in mare's colostrum and milk during the firs...
Peterson ML, Srinath S, Murphy J.A new instrument is presented which is capable of high resolution acoustic imaging at relatively low frequencies. This approach results in increased complexity of the signal processing required and reduced throughput of the instrument. However, these disadvantages are amply compensated by the ability to create velocity scan images of materials with either high attenuation or low material velocities. These measurements are not possible using traditional/acoustic microscopes. The initial performance of the new instrument is demonstrated using thin samples of shim materials to show that acceptabl...
Costa LR, Spier SJ, Hirsh DC.Ribotyping and susceptibility to 17 antimicrobial agents were used to compare 37 isolates of Corynebacterium pseudotuberculosis (28 from horses, 1 from cattle, 3 from sheep and 5 from goats) derived from various types of lesions, and different geographic locations. According to the presence of nitrate reductase, all but one isolate from horses reduced nitrate (nitrate-positive), whereas all isolates from sheep and goats were unable to reduce nitrate (nitrate-negative). The ribotype of the nitrate-negative isolate from a horse with ulcerative lymphangitis was identical to all the other isolates...
Mikami T, Tsuchihashi K, Kashiwagi M, Yachida Y, Daino T, Hashi K, Akino T, Gasa S.A sulfatide, O-fatty-acylated 3-sulfogalactosylceramide at C6-O on galactoside, was isolated from equine brain and the chemical structure was characterized by proton NMR and MS. The O-acylation site of the acylated sulfatide was determined by the down-field shift of protons attached to a carbon having an O-acyl group in the NMR spectrum and by analysis of a partially methylated derivative before and after acetalization of the intact sulfatide using GC-MS. The O-acyl chain length was determined by GLC, revealing that it exclusively had palmitoyl and stearoyl residues as the major fatty acids. T...
Holland JL, Kronfeld DS, Sklan D, Harris PA.Marker methods are needed for estimating fecal output by grazing animals in studies of nutrition and environmental impact. In addition, estimates of prefecal mass and turnover time are relevant to exercise performance and certain digestive disorders. As a first step in developing marker methods for field use, a chromic oxide model of fecal kinetics was developed and tested in the context of a digestion balance experiment with stall-fed horses. The model consists of removal of feces at a constant rate from a single compartment, the prefecal mass. Four horses were fed hay, and another four were ...
Paterson DL, Murray PK, McCormack JG.Twenty-three horses and three humans in Queensland, Australia, were infected with a novel member of the Paramyxoviridae family of viruses in two geographically distinct outbreaks. Two of the humans died-one died of rapid-onset respiratory illness, and the other died of encephalitis. The third infected human developed an influenza-like illness and made a complete recovery. All infected humans had close contact with sick horses. Since the two outbreaks occurred at sites 1,000 km apart and no known contact between the two groups of humans and horses occurred, extensive testing of animals and bird...
Cheng FP, Fazeli AR, Voorhout WF, Tremoleda JL, Bevers MM, Colenbrander B.The aim of this study was to investigate whether mare follicular fluid (FF) induces the acrosome reaction (AR) in stallion spermatozoa and, if so, to identify the component in FF responsible for it. Furthermore, the effect of this component on sperm-zona binding and the subsequent AR was studied. Pooled FF, aspirated from the preovulatory follicles of mares in oestrous, was used and aliquots of the fluid were treated with charcoal to remove steroids (CFF). Charcoal treatment reduced the progesterone concentration in FF from 153 to < 2 ng/mL. Spermatozoa from fertile stallions collected by a...
Wilsdorf G, Graf G.Since the early twenties several institutes of the Veterinary College in Berlin were involved in the development of microchemical analyzing methods for doping-alkaloids and clinical studies in doped race horse. This research made it possible to build national acting structures against the abuse of doping in race horses, which soon became a model for similar activities in other countries, for instance Greece, Japan and the United States. Concerning the results of their research, the scientists of the Veterinary College in Berlin have got an enormous part in fighting against doping in race horse...
Flachowsky G, Kirchgessner M.The paper describes the energetic feed evaluation systems for ruminants, pigs, poultry and horses presently used in Germany. During the last ten years the "Ausschuss für Bedarfsnormen" (AfB; Committee of Nutrient Requirements) of the "Gesellschaft für Ernährungsphysiologie" (GfE, Society of Nutritional Physiology) introduced new recommendations for energy requirements of domestic animals including national and international references. The energetic requirements were factorially deduced (demand for maintenance and various performances) under consideration of partial efficiency of utilized m...
Douglas JE, Biddick TL, Thomason JJ, Jofriet JC.The equine laminar junction plays a vital role in transferring the forces of weight-bearing between the epidermal hoof wall and the bone of the third phalanx, but the way in which it performs this function is poorly understood. Using samples from sites varying proximodistally and circumferentially around the hoof, the stress/strain behaviour of this tissue was characterised in three directions: radial tension and proximodistal and mediolateral shear. The influences of toe angle and length were also examined. For all three test directions, the modulus of elasticity increased with increasing str...
Ebert DW, Roberts C, Farrar SK, Johnston WM, Litsky AS, Bertone AL.Measurements of absolute total reflectance were recorded from weight-bearing (n=9) and nonweight-bearing (n=9) equine articular cartilage specimens from 300 to 850 nm using a spectrophotometer with integrating sphere attachment. Following correction of measured spectra for interfacial reflections and edge losses, Kubelka-Munk theory was applied to estimate absorption and scattering coefficient, one-dimensional light intensity distribution, and light penetration depth. Kubelka-Munk absorption coefficients ranged from ∼7 cm-1 at 330 nm to ∼1 cm-1 at 850 nm. A localized absorption peak wa...
Tyler CM, Golland LC, Evans DL, Hodgson DR, Rose RJ.Thirteen standard-bred horses were trained intensively for 34 weeks and detrained for 6 weeks to study skeletal muscle adaptations to prolonged training, overtraining and detraining. Training included endurance (phase 1, 7 weeks), high-intensity (phase 2, 9 weeks) and overload training (OLT) (phase 3, 18 weeks). During phase 3, horses were divided into two groups, OLT and control (C), with OLT horses performing greater intensities and durations of exercise than C horses. Overtraining was evident in OLT horses after week 31 and was defined as a significant reduction in treadmill run time in res...
Khalil AM, Murakami N, Kaseda Y.Jugular vein blood samples were collected from 23 young and sexual mature feral stallions to examine the relationship between plasma testosterone concentration and age, breeding season or harem size. Testosterone concentration increased with the age of the stallions until they formed their own harems, at about 4 to 6 years old. Seasonal variations in testosterone concentrations were observed, and found to be significantly higher (P<0.001) throughout the breeding season than non-breeding season, from 3 years of age. Testosterone levels were correlated with harem size for individual stallions...
Howard RD, McIlwraith CW, Trotter GW, Nyborg JK.To clone equine interleukin 1 alpha (IL-1 alpha) and equine interleukin 1 beta (IL-1 beta) and determine their full-length cDNA sequences. Methods: The mRNA isolated from lipopolysaccharide-stimulated cultured equine monocytes was reverse transcribed, and a cDNA library was constructed in a lambda phage. The cDNA library was screened by means of plaque hybridization with radiolabeled human IL-1 alpha and IL-1 beta cDNA probes. The cDNA nucleotide sequences for equine IL-1 alpha and equine IL-1 beta were determined by use of the dideoxy chain termination technique. The cDNA sequences were analy...
Lochner HL, Martinson KL, Bianco AW, Hutchinson ML, Wilson ML, Johnston LJ, Dentzman KE.Challenges associated with burial, rendering, and cremation have forced horse owners to seek alternative mortality disposal methods. While equine mortality composting has been successfully demonstrated, industry-wide adoption has been limited. Therefore, evaluation of horse owners' and veterinarians' perceptions and experience with mortality composting is needed. Two surveys were developed to evaluate industry practices and decisive factors regarding equine euthanasia and mortality disposal methods. Each survey was designed for a separate audience: horse owners or veterinarians serving equines...
Shin HD, Suh JH, Kim J, Cho HD, Lee SD, Han KS, Wang Y, Han SB.A high throughput method for simultaneous screening of anabolic steroids and their metabolites (4-esterendione, trenbolone, boldenone, oxandrolone, nandrolone, methandrostenolone, testosterone, 1-androstendione, ethisterone, normethandrolone, methyltestosterone, 16β-Hydroxystanozolol, epitestosterone, bolasterone, norethandrolone, danazol, stanozolol and androstadienone) in equine urine by online turbulent flow extraction coupled with liquid chromatography-tandem mass spectrometry was developed. The use of turbulent flow chromatography could simplify pretreatment of horse urine, which has com...
Smith RM, Kotzé A, Grobler JP, Dalton DL.Toll-like receptors (TLR) are a family of proteins that signal activation of the innate immune response through the recognition of a variety of pathogen molecular compounds. Here, we characterized the complete TLR9 gene in Cape mountain zebra (Equus zebra zebra) from three populations in South Africa and compared sequences to a variety of horse and donkey breeds. Overall, we identified six single nucleotide polymorpHisms (SNPs). A single SNP (G586S) was non-synonymous, whereas the remaining SNPs were synonymous. The G586S alteration was detected in Cape mountain zebra populations with varying ...
Mizuno Y, Aida H, Hara H, Fujinaga T.Intermittent positive pressure ventilation (IPPV) is useful method for compensate of respiratory function in anesthetized horses. However, IPPV may decrease cardiac output. Alterations in cardiac output of three groups (N = 5) healthy, halothane-anesthetized mares were determined and compared during a 120 min period of anesthesia. The groups were as follows: spontaneous ventilation (SV), controlled ventilation using an end-inspiratory pressure of 20 cmH2O (CV20) and a third group using 25 cmH2O (CV25) inspiratory pressure. In the CV groups, respiratory function was adequately maintained. Altho...
Galuppo LD, Stover SM, Aldridge A, Hewes C, Taylor KT.To compare monotonic mechanical properties of gap-ostectomized third metacarpal bones (MC3) stabilized with an MP35N interlocking nail system with contralateral intact bones. Methods: Twenty-four pairs of cadaveric equine MC3s. Methods: Third metacarpal bones were divided into 4 mechanical testing groups (6 pairs per group): compression, palmarodorsal (PD) and mediolateral (ML) 4-point bending, and torsion. One MC3 from each pair was randomly selected as an intact specimen, and the contralateral gap ostectomized bone was stabilized with a 4-hole, 14-mm-diameter, 250-mm-long, MP35N intramedulla...
Maia L, Dias MC, de Moraes CN, de Paula Freitas-Dell'Aqua C, da Mota LS, Santiloni V, da Cruz Landim-Alvarenga F.Cryopreservation is a feasible alternative to maintaining several cell lines, particularly for immediate therapeutic use, transportation of samples, and implementation of new in vitro studies. This work parts from the hypothesis that the medium of cryopreservation composed by 90% of conditioned medium (CM) supports cryopreservation of equine umbilical cord intervascular matrix mesenchymal stem cells (UCIM-MSCs), allowing the maintenance of the biological properties for the establishment of cell banks intended for therapeutic use and in vitro studies. Thus, we evaluated the viability, apoptosis...
Rungsipipat A, Tateyama S, Yamaguchi R, Uchida K, Miyoshi N.An immunohistochemical study of various visceral organs of normal adult dogs, cats, pigs, horses, cows, and chickens (five of each species) and of 185 spontaneous canine tumours was carried out using paraffin wax sections and a commercially available antibody to the human c- yes oncogene product. Among the adult normal tissues of six animal species, epithelial cells of the proximal and distal renal tubules, the myocardium, hepatocytes, cerebellar Purkinje cells and adrenal cortical cells were positive for c- yes product. Among the foetal tissues of dogs and chickens, a positive reaction was ob...
Back W, Smit LD, Schamhardt HC, Barneveld A.To study the influence of different exercise regimens on the development of locomotion, 40 Warmblood foals aged 1 week were subdivided into 3 groups: box-rest, training and pasture exercise. The box-rest group remained for 24 h a day in a box stall while the training group was housed similarly, but additionally received a 30 min workout with gallop sprints 6 times a week. The pasture group served as a control group and was kept at pasture for 24 h a day. After 5 months, the locomotion pattern at the trot of every foal was recorded overground with a 2-D MacReflex gait analysis system. A randoml...
Warren-Smith AK, McGreevy PD.Recently, training horses within round-pens has increased in popularity. Practitioners often maintain that the responses they elicit from horses are similar to signals used with senior conspecifics. To audit the responses of horses to conspecifics, 6 mare-young-horse dyads, this study introduced them to each other in a round-pen and videoed them for 8 min. These dyads spent significantly more time farther than 10 m apart than they did less than 1 m apart (p < .001). The time they spent less than 1 m apart decreased over the 8-min test period (p = .018). Mares occupied the center of the roun...
López ML, Olea N, Retamal CA.Freeze-fracture replicas of stallion spermatozoa, collected from the proximal caput, corpus and cauda epididymides regions, were analyzed by electron microscopy to explore the distribution and density of intramembrane particles (IMP). Conspicuous differences in density and arrangement of the IMP were observed in the different topographical domains of mature and immature spermatozoa. A reduction of IMP, especially remarkable in the post-acrosomal domain, was observed in mature epididymal spermatozoa when compared with samples collected from ductuli efferentes. Some structural species-specific d...
Jaggard G.The major problems of racing in the United States at the present time are caused by too much racing. This has led to too few horses and small fields. Consequently many owners and trainers are trying to enter their horses too frequently and to race them when they are not really fit to run. The desire to race horses as frequently as possible has led to constant pressure from horsemen through their organizations for so called "permissive medication". Started in the state of Colorado approximately ten years ago this has grown until finally there are only a few states, notably New York and New Jers...
Greening L, Allen S, McBride S.Sleep disturbance is observed across species, resulting in neurocognitive dysfunction and poor impulse control/regulation of negative emotion. Understanding animal sleep disturbance is thus important to understand how environmental factors influence animal sleep and day-to-day welfare. Self-reporting tools for sleep disturbance are commonly used in human research to determine sleep quality, that cannot be transferred to non-verbal animal species research. Human research has, however, successfully used frequency of awakenings to create an objective measurement of sleep quality. The aim of this ...
Taverne MA.For the past few years, two-dimensional diagnostic ultrasound methods have become available to veterinary medicine. In particular, linear-array real-time ultrasound would appear to be a very suitable diagnostic tool in the examination of the female genital organs of domestic mammals. The principles of this technique and the equipment available are described, and particular attention is paid to the method by which a diagnosis of pregnancy may be established in various animal species. In dogs and goats shaving will have to be done before the sound-transmitting/receiving transducer can be placed ...
Zeyner A, Kirbach H, Fürll M.Feeding a high-fat diet may have potential advantages by maintaining pH homeostasis during intense exercise; conversely, effects including elevated PCV and increased plasma concentration of total protein may indicate effects detrimental to performance by reducing perfusion of tissues and organs. Six horses were used to determine the effects of an replacement of starch (diet '0.00') with 0.33, 0.67, 1.00 and 1.33 g soybean oil/kg bwt/day (diets '033', '0.67', '1.00' and '1.33') on venous and renal acid-base status and fractional clearance (FC) of electrolytes in a postprandial state but prior t...
Ren WX, Zhang XX, Long CY, Zhao Q, Cheng T, Ma JG, Xu P, Hou G, Ni HB.Horse meat and milk are an important source of nutrition for many Chinese. Previous studies have shown a fairly high prevalence of infection in horses. However, there is no data concerning the molecular characterization of in horses in China. The present study tested 231 cervical lymph node samples of horses from northern China (97 from Jilin, 54 from Liaoning, and 80 from Xinjiang) for the presence of the B1 gene by seminested PCR. The B1-positive samples were genotyped at nine nuclear loci, that is, SAG1, alternative SAG2, SAG3, BTUB, c22-8, GRA6, c29-2, PK1, and an apicoplast locus, usin...
Padilla AW, Tobback C, Foote RH.A method for preparing stored unfrozen stallion spermatozoa for the zona-free hamster oocyte penetration test (HOPT) and a subsequent comparison of fresh and stored sperm by the HOPT were evaluated. In Experiment 1, sperm from 4 stallion ejaculates, cooled to 4 degrees C and stored for 24 h, were treated with 60, 90 and 120 microM of dilauroylphosphatidyl-choline (PC12) liposomes to initiate the acrosome reaction. The percentage of motile and acrosome-reacted (AR) sperm were recorded after 8, 15 and 30 min of incubation at 39 degrees C, by automated image analysis. Liposome concentration did n...
Zhang XY, Robinson NE, Zhu FX.We tested the hypothesis that increasing intracellular levels of adenosine 3', 5'-cyclic monophosphate (cAMP) increases acetylcholine (ACh) release from airway parasympathetic nerves. Muscle strips from equine trachea were preincubated for 60 min with 10(-7)M atropine, 10(-6)M neostigmine, and 10(-5) M guanethidine. The ACh release was evoked by electrical field stimulation (EFS, 20 V, 0.5 ms, 0.5 Hz) and measured by high-performance liquid chromatography with electrochemical detection. Agents known to increase cAMP, i.e., forskolin (10(-6) - 10(-4) M), 8-bromoadenosine 3', 5'-cyclic monophosp...
Asseged BD, Habtemariam T, Tameru B, Nganwa D.Deriving horse oocytes in the USA is hampered by the lack of abattoirs processing horse carcasses which could provide abundant quantities of ovaries from slaughtered mares. Therefore, several cloning industries in the USA are attempting to import cloned horse embryos from Canada. Like any agricultural commodity, cloned embryos pose a risk of introduction of exotic animal diseases into the importing country. Under such circumstances, risk assessment could provide an objective, transparent, and internationally accepted means for evaluating the risk. This quantitative risk assessment (QRA) was in...
Malik CE, Wong DM, Dembek KA, Wilson KE.To determine the accuracy of 2 interstitial glucose-monitoring systems (GMSs) for use in horses compared with a point-of-care (POC) glucometer and standard laboratory enzymatic chemistry method (CHEM). Methods: 8 clinically normal adult horses. Methods: One of each GMS device (Dexcom G6 and Freestyle Libre 14-day) was placed on each horse, and blood glucose concentration was measured via POC and CHEM at 33 time points and compared with simultaneous GMS readings. An oral glucose absorption test (OGAT) was performed on day 2, and glucose concentrations were measured and compared. Results: Glucos...
Siciliano PD, Wood CH.Fourteen 2-yr-old Quarter Horses and Quarter Horse x Thoroughbreds were randomly assigned to either a control (CTRL) diet or a diet supplemented with 6.4% soybean oil (SBO). The amounts of both diets that were fed met current NRC nutrient requirements and were isoenergetic with similar nutrient:energy ratios. Venous blood samples (20 mL) were taken at d 0, 30, 60, and 90 of the experiment and analyzed for serum alpha-tocopherol, serum cholesterol, and serum triglyceride. The sum of serum cholesterol and serum triglyceride values was used as an estimate of serum total lipid. The ratio of serum ...
Miyashita Y, Nakajima S, Imada H.Panel-touch behavior of 3 geldings was successfully established by a response-termination type of autoshaping procedure. An omission or negative contingency introduced after the training of an animal, however, decreased the response rate to a near-zero level.
Villar E, Calvo P, Cabezas JA.1. Peripheral blood serum alpha-L-fucosidases have been studied from various mammalian species: Sus scropha var domestica L. (pig), Capra hircus L. (goat), Bos taurus L. (bull, races Morucha and Charolais), Equus caballus L. (horse) and Equus asinus L. (donkey). 2. Fluorimetric and spectrophotometric procedures were used for determination of alpha-L-fucosidases. 3. alpha-L-Fucosidases were more active towards fluorescent substrates than towards chromogenic substrates. 4. pH optima values of the enzymes are: (A) 5.5 for sera from all above-mentioned species when fluorescent substrates were empl...
Bleyaert HF, Madison JB, Bailey JE, Johnson CM.Six ponies divided into two groups of three were used in a double crossover study design. Group 1 ponies had a small intestinal resection and anastomosis performed using a biofragmentable anastomosis ring (BAR); group 2 ponies had a hand-sewn small intestinal resection and anastomosis using a Gambee suture pattern. Approximately 30 days later, all ponies had a second celiotomy and anastomosed segments were removed. Group 1 ponies had a hand-sewn anastomosis performed and group 2 had a BAR. The anastomotic sites were collected at necropsy approximately 30 days later. Anastomosed intestinal segm...
Barsoum IS, Botros BA, Morcos MB.In a serologic survey on equine leptospirosis in Egypt, the following incidences of leptospiral serosensitivity were found: 1. Hospitalised horses 65/113 (57.5 %). 2. Hospitalised donkeys 90/125 (72.0 %). 3. Apparently healthy horses 21/72 (29.1 %). Sera of these animals were mostly reacting to serotypes butembo, pomona, icterohemorragiae, and grippotyphosa. Equine in Egypt are close animals to humans and may constitute a potential source of leptospiral infection. From the clinical point of view, it is very possible that ocular, hoof lesions and icterus in equines would be expected with leptos...
Longland AC, Barfoot C, Harris PA.Obesity can negatively impact upon equine welfare and bodyweight (BW) of pastured equines is often difficult to manage. Objective: To compare the effects of three restricted grazing regimens on changes in pony BW and morphometric measurements. Methods: Randomised study. Methods: Twelve mature ponies were individually grazed in 10m wide, rectangular, electric fenced paddocks. The dry matter (DM) herbage yield of each paddock was determined, and paddock length adjusted on Day 1 to provide 1.5% of individual pony BW as herbage DM daily, for 28 days. There were four ponies per treatment. Treatmen...
Chevalier H, Posner LP, Ludders JW, French TW, Erb HN, Gleed RD.To determine accuracy and precision of a point-of-care hemoglobinometer for measuring hemoglobin concentration and estimating PCV in horses. Methods: Prospective trial. Methods: 55 horses. Methods: Blood samples were obtained from 43 horses examined at a veterinary teaching hospital. Hemoglobin concentration was measured with the hemoglobinometer and by means of the standard cyanmethemoglobin method; PCV was measured by centrifugation. Blood samples were also obtained from 12 healthy horses, and PCV of aliquots of these samples was altered to approximately 5 to 80% by removing or adding plasma...
Sätter JK, McGawley K, Connysson M, Staunton CA.To identify how riding rein direction (left and right) and rider asymmetry affect tölt performance in Icelandic horses. Two horses were ridden in tölt by four riders on both left and right reins. Riders wore pressure insoles that measured the total absolute force (FAbs) and absolute force difference (FDiff) in their left and right feet in the stirrups. A 3D motion-analysis system recorded the degrees of side-to-side movement in the pelvis (RollP) and in the thoracolumbar region (RollT). Lateral advanced placement (LAP) and duty factor (DF) were calculated to determine tölt performance. One-...
Bampidis V, Azimonti G, Bastos ML, Christensen H, Durjava M, Dusemund B, Kouba M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the safety and efficacy of DBVPG 48 SF (BioCell®) as a zootechnical feed additive for horses, pigs and ruminants. The product, manufactured in three formulations (microsphere, micropellet and powder), is intended for use in complete feed at a minimum inclusion level of 3 × 10 CFU/kg complete feed for horses, 4 × 10 CFU/kg complete feed for dairy cows and minor dairy species, 4 × 10 CFU/kg complete feed for calves, cattle for fattening, minor growing and fattening ruminants...
Mikami T, Tsuchihashi K, Kashiwagi M, Yachida Y, Daino T, Hashi K, Akino T, Gasa S.A sulfatide, O-fatty-acylated 3-sulfogalactosylceramide at C6-O on galactoside, was isolated from equine brain and the chemical structure was characterized by proton NMR and MS. The O-acylation site of the acylated sulfatide was determined by the down-field shift of protons attached to a carbon having an O-acyl group in the NMR spectrum and by analysis of a partially methylated derivative before and after acetalization of the intact sulfatide using GC-MS. The O-acyl chain length was determined by GLC, revealing that it exclusively had palmitoyl and stearoyl residues as the major fatty acids. T...
Guimarães T, Lopes G, Ferreira P, Leal I, Rocha A.Cryopreservation of epididymal spermatozoa is a useful tool to preserve genetic material of valuable stallions after emergency castration or unexpected death. For that, testicles and epididymides are generally sent refrigerated to the laboratory. Collection of epididymal spermatozoa is a simple procedure that reduces the volume of the material to be shipped, and may improve the quality of the chilled epididymal sperm cells. In the present study we compared the characteristics of frozen/thawed epididymal spermatozoa after refrigeration of the epididymis or after direct refrigeration of the exte...
