Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
BRUCE LW, SHORLAND FB.IN the liver lipids of animals which have so far been examined it has been found1 that the phospholipids, as compared with the glycerides, contain less hexadecenoic acid but more stearic and highly unsaturated C20 and C22 acids, the latter being derived from dietary linoleic and linolenic acids2. Phospholipids, moreover, are generally regarded as selecting the more highly unsaturated acids from the diet3.
da Silva GM, Navas C, Carvalho MB, Sampaio Y, Rocha MN, Gomes-Silva A, de Matos Guedes HL, Cunha L, Castilho LR, da Silva JL, Felicori LF.Horse immunoglobulins have been utilized for over a century in serotherapy to treat venomous animal bites and various other conditions. However, molecular-level information about these immunoglobulins remains limited, particularly regarding immunoglobulin lambda light chains (Igλ), which constitute over 90 % of circulating antibodies. Despite the sequencing of the equine genome, the International ImMunoGeneTics information system (IMGT) has not yet annotated Igλ in its database, restricting the analysis of the horse antibody repertoire. In this study, we analyzed the equine Igλ repertoire...
Warren P, Mc Crudden D, O'Reilly S, Mc Eleney C.Macroalgae have been used in supplements and feedstuffs for equines and other animals for many years. Health-related attributes of macroalgal bioactive compounds, including antioxidant, anti-inflammatory and anthelmintic effects, have been largely reported in non-equine species to date. Given the prevalence of resistance to antimicrobial and anthelmintic drugs, sourcing naturally occurring alternatives is of high priority in the current One Health strategy. Nutritionally, seaweed contains a broad range of macro and micro nutrients. These nutrients, combined with the bioactive compounds such as...
Döberl J, Li Z, Furrer R, Jäger MC.Reduced anthelmintic efficacy against small strongyles in horses is a widespread problem, yet recent data is scarce. Objective: This paper aims to investigate the current status of anthelmintic efficacy in the field in Germany, specifically focusing on pyrantel. Methods: The study included 1670 horses for which a total of 1913 dewormings were analysed. Of these, 1682 were performed with pyrantel, 56 with fenbendazole, 162 with macrocyclic lactones and 13 with other compounds (e.g. herbs). The faecal egg count reduction (FECR) was calculated with two methods, the faecal egg count reduction test...
Jamshidpour R, Nabavi R, Moadab H, Rezaie F, Chale AC, Sargison N.Resistance to benzimidazole (BZ) by cyathostomin nematodes has become a major threat to equine health around the world. We aimed to evaluate the efficacy of BZ drugs against small strongyle nematodes in horses of western Iran using coprological and molecular examination. Unassigned: Faecal egg count reduction tests were performed on 398 horses kept in 16 stables in western Iran (Kermanshah Province), to detect benzimidazole resistance in small strongyle nematodes. Allele-specific PCR was used to identify the F200Y (TAC/TTC) SNP in the beta-tubulin gene codon in cyathostomin larvae. Unassigned:...
Mainguy-Seers S, Grondin SM, Lavoie JP.Airway dysfunction in severe equine asthma (SEA) often results in early retirement or euthanasia of affected horses. Exercise-induced bronchodilation occurs in horses with SEA after intense treadmill exercise, but the effects of a lighter, field-applicable, training regimen remain largely unexplored. Objective: To evaluate the impact of submaximal aerobic exercise on airway obstruction during exacerbation of SEA. Methods: The preliminary phase explored the effects of a 25-min standardised exercise on the lung function of eight SEA horses. As notable bronchodilation occurred, the results were c...
Rimayanti R, Khairullah AR, Mustofa I, Utomo B, Lestari TD, Utama S, Akintunde AO, Mulyati S, Hernawati T, Dawood AQ, Riady G, Khan IU, Rasad SD.... is a protozoan parasite that causes the sexually transmitted disease known as "dourine" in horses. This chronic illness is directly spread from one animal to another during mating. Doflein proposed the name in 1901. Despite being distributed worldwide, the broad use of artificial insemination technology over the past three decades has resulted in only a few cases being documented. The condition is typically fatal and is characterized by gradual emaciation, nervous system involvement, and edematous lesions of the genitals. The incubation period between exposure and the onset of clinical sympt...
Piecuch A, Szterk A.This review highlights the role of β-glucans-specifically β(1 → 3), β(1 → 6), and β(1 → 4)-glucans-as water-soluble dietary fibres in animal nutrition and health. Sourced from yeast, plants, seaweed, and mushrooms, β-glucans differ in structure depending on origin, which affects their solubility and biological activity. Recent studies emphasize their therapeutic potential in horses, particularly for managing gastric ulcers, viral infections, immune dysfunction, and poor performance. The review summarizes data on effective dosages, formulations, and sources of β-glucans tailor...
St George L, Nankervis K, Walker V, Maddock C, Robinson A, Sinclair J, Hobbs SJ.Despite growing evidence on the adaptive movement patterns that horses adopt during water treadmill (WT) exercise, underlying adaptations in muscle activity remain uninvestigated. This feasibility study aimed to develop a method for the synchronous measurement of muscle activity and movement of horses during WT exercise. Combined surface electromyography (sEMG) (2000 Hz) from selected hindlimb (biceps femoris, gluteus medius, tensor fasciae latae) and epaxial (longissimus dorsi) muscles, and three-dimensional kinematic (200 Hz) data from the back and pelvis of one (n = 1) horse were collected ...
Rump-Dierig I, Giers J, Frenzel C, Stöckle S, Gehlen H.This study examines seasonal changes in muscle and heart parameters in eventing horses over the course of a competition season. Blood levels of the enzymes creatine kinase (CK) and aspartate aminotransferase (AST) as well as the heart muscle-specific concentration of cardiac troponin I (cTnI) were measured before (pre), 30 min (p30) and 24 h (p24) after competitions. Creatine kinase (CK: median pre-competition = 175 U/L, 30 min post = 221 U/L, 24 h post = 140 U/L), aspartate aminotransferase (AST: pre = 319 U/L, p30 = 335 U/L, p24 = 333 U/L), and cardiac troponin I concentration (cTnI: pre = 0...
Kyle-LaBell E, Andersson A, Baughman B.Fibrocartilaginous embolic myelopathy (FCE) was diagnosed in a 15-year-old pony mare by histopathologic examination of the spinal cord. The horse was euthanized and submitted for necropsy following focal seizures and lateral recumbency for approximately 12 h. Histopathology of spinal cord segments C5-C7 revealed severe ischemic myelomalacia with intravascular amorphous material that stained positive with Alcian Blue, consistent with cartilaginous matrix. These features in addition to the acute onset of clinical signs are characteristic for FCE. This case report describes the clinical and histo...
Racine J, Ungermann A, Blatter M, von Plato G, Rijkenhuizen ABM.To describe a modified two-portal tenoscopic technique for proximal manica flexoria (MF) resection designed to preserve the mesotendons (MTs) of the deep digital flexor tendon (DDFT). Methods: Prospective clinical study. Methods: Client-owned horses (n = 30). Methods: MF resection was performed in four steps: (1) Transection of the lateral attachment of the MF to the superficial digital flexor tendon (SDFT), staying abaxial to the lateral MT; (2) transection of the medial attachment of the MF to the SDFT between the flexor tendons from distal to proximal until reaching the level of the med...
Serighelli-Júnior G, Strugava L, de Oliveira Franco JD, Kulik K, Dornbusch PT, Machado M, Moreno JCD.To describe the detailed anatomy of the equine thoracoabdominal region and to compare the accuracy of ultrasound-guided versus blind intercostal nerve injection techniques, targeting the thoracic intercostal nerves (T12-T18) in equine cadavers. Methods: Randomized, blinded, cadaveric study. Methods: Eight fresh adult equine cadavers. Methods: Eight horse cadavers were used: one in phase I (436 kg; 4 years old) for anatomic landmarks identification and seven in phase II [420 (317-560 kg); 16 (3-22 years old) [median (minimum-maximum)] for comparative study. In phase I, dissections were performe...
Góss GC, Mozzaquatro FD, Machado IRL, Gomes EM, Martins NR, Duarte CA.The collateral ligaments of the distal interphalangeal joint (CL-DIPJ) are important structures for athletic horses. Ligament injuries can cause lameness, but ultrasound imaging can detect such lesions, which usually present with changes in echogenicity and an increase in the cross-sectional area (CSA) of the ligament. Measurements of these structures vary among authors, which complicates the diagnosis. Thus, this study aimed to describe the ultrasound measurements (dorsopalmar diameter [DPD], lateromedial diameter [LMD], and CSA) of the CL-DIPJ in Argentine Polo horses. We measured the CL-DIP...
Stokes C, Natalini CC, Wills R, Eddy A, Mochal-King C, O'Shea C, Fontenot RL.To evaluate the analgesic properties and adverse effects of liposomal bupivacaine administered via caudal epidural in horses. Methods: Pharmacodynamic study. Methods: A total of 10 healthy adult horses. Methods: Liposomal bupivacaine (10 mL; 1.3%) was administered into the caudal epidural space via an indwelling epidural catheter inserted to a length of 15 cm. Mechanical nociceptive threshold (MNT) testing with pressure algometry was used to measure analgesia at four anatomic locations for 72 h following injection. Physical examinations (including rectal temperature, heart rate, respirat...
Alexeenko V, Anchan DS, Ter Woort F, Ribonnet C, van Erck E, Marr C, Jeevaratnam K.Non-linear equine electrocardiography (ECG) analysis is an actively developing study area which has the potential to lead to novel, artificial intelligence-based diagnostic tools in equine cardiology. As more ECG recording devices are becoming available, there is a need to ensure results are interchangeable regardless of the equipment used to record the equine ECG. Objective: To evaluate the agreement of ECG complexity values obtained using the Televet™ and Equimetre™ systems. Methods: Cross-sectional clinical. Methods: ECGs were recorded using two devices simultaneously from 37 healthy Th...
Sala-Ayala L, Cuervo-Arango J, Martínez Boví R.Endometrial edema is an ultrasonographic sign of estrus in mares and is positively associated with fertility. This study aimed to evaluate the influence of follicular size, systemic progesterone (P4), and exogenous estradiol benzoate (EB) on the onset and duration of endometrial edema. In Experiment 1, anestrus (n = 4) and diestrus mares (n = 4) received 4 mg of EB intramuscularly; edema scores and plasma P4 were monitored over four days. In a complementary group (n = 5), mares were monitored until ovulation, then administered 4 mg of EB and examined every 12 h for 48 h. In Experimen...
Martin-Pelaez S, Orsolini M, Verstraete M, de la Fuente A, Takahashi K, Meyers S, Dini P.Equine in vitro embryo production (IVP) offers a valuable opportunity to enhance the efficient utilization of genetically and economically valuable horses. In cases of subfertility, sudden death, emergency euthanasia, or limited sperm availability, IVP becomes the only viable option to produce offspring. Due to steadily improving success rates, IVP has become one of the most sought-after assisted reproductive techniques. The rising global demand has created a clear need for veterinarians and stallion owners to provide IVP-suitable sperm, where the selection of intact sperm for intracytoplasmic...
Sabzpoosh M, Hoveizi E, Gooraninejad S.This study aimed to extract and isolate endometrial stromal cells from Arabian mares and investigate their growth and differentiation potential. Endometrial biopsies were obtained from three healthy 6-year-old Arabian mares using a standardized, minimally invasive protocol. The isolated cells were characterized using flow cytometry and differentiation analysis. Flow cytometry revealed mesenchymal markers CD90 (95.2%) and CD105 (97.4%) and hematopoietic markers CD34 (1.17%) and CD45 (0.339%). The cells exhibited differentiation potential into adipocytes, osteoblasts, and chondrocytes. The findi...
Zuluaga-Cabrera AM, Barbosa da Costa G, Martinez ID, Arias MP.Colombian Paso horses (CPs) excel in gait competitions due to their endurance, speed, and precision, which demand a high level of cardiorespiratory fitness. However, their training regimes often lack scientific support, and few studies have linked physiological parameters to optimal training zones. This study aimed to estimate the aerobic lactate threshold (LTaer) using mathematical methods and to correlate it with heart rate (HR) zones and blood lactate. Eighteen CPs were evaluated and classified into trained (GT) and untrained (GD) groups. All animals underwent a field-based incremental exer...
François AC, Taminiau B, Renaud B, Gonza-Quito IE, Massey C, Hyde C, Piercy RJ, Douny C, Scippo ML, Daube G, Gustin P, Delcenserie V, Votion DM.Hypoglycin A is a plant-derived protoxin that causes atypical myopathy in equids. In atypical myopathy-affected horses, metabolomic and microbiome studies have reported alterations in metabolic markers and faecal microbiota composition, pointing to a potential disruption of microbial homeostasis. However, in vivo observations are strongly confounded by host-related factors, underscoring the need for controlled in vitro approaches. To address this, we used an in vitro static batch fermentation model simulating the equine colon to investigate the direct effects of hypoglycin A on microbiota comp...
Varandas M, Lozano J, Agrícola R, Gomes L, Rosa T, Magalhães M, Lamas L, Rinaldi L, Oliveira M, Paz-Silva A, Madeira de Carvalho L.The diagnosis of gastrointestinal (GI) strongyle infections in equids is still mainly performed using quantitative coprological techniques, like the McMaster (McM), but more sensitive and precise techniques, like FLOTAC (FL) and Mini-FLOTAC (MF), have been proposed over the past 20 years. The present study aimed to compare the analytical performance of these three methods in the diagnosis of strongyle infections in horses. Between October 2023 and June 2024, 32 fecal samples were processed using the McM, FL and MF techniques to identify strongyles' eggs, estimate their shedding (eggs per gram ...
Fouché N, Howard J, Gerber V, Billmann P, Farinha do Sul M, Christen G, Bruckmaier R, Philipona C, Schmutz NB, Gross J.β-endorphin, a proopiomelanocortin (POMC)-derived peptide secreted by pars intermedia melanotropes, may play a significant but underexplored role in pituitary pars intermedia dysfunction (PPID) pathophysiology and diagnosis. This study aimed to (1) validate a commercially available human β-endorphin enzyme-linked immunosorbent assay (ELISA) kit for equine samples, and (2) compare β-endorphin concentrations between horses with PPID and healthy controls. Assay validation included the generation of standard curves using purified synthetic equine β-endorphin and human β-endorphin standards, w...
Haugaard SL, Schneider MJ, Kjeldsen ST, Sattler SM, Bastrup JA, Saljic A, Birk JB, Hansen C, Synnestvedt JN, van Hunnik A, Sobota V, Carstensen H....Horses are one of the few animals that spontaneously develop atrial fibrillation (AF), making them a powerful model for studying AF mechanisms and treatment effects. Despite the initial effectiveness of treatment in horses and humans, AF-induced atrial remodeling compromises its long-term success. Observational studies have suggested that metformin may reduce the risk of AF, but its effects on progressive AF-induced atrial remodeling have yet to be evaluated in a high-fidelity large animal model. Unassigned: Here, we used a longitudinal horse model of tachypacing-induced self-sustained AF to c...
Khalil WA, Mostafa HE, Derbala MK, Alfattah MA, Alhujaili W, Hassan MAE, El-Harairy MA, Abdelnour SA.Encapsulating natural antioxidants presents a robust strategy to neutralize oxidative stress, thereby improving semen preservation. Therefore, this study aimed to evaluate the efficacy of butylated hydroxytoluene nanoparticles (BHT-NPs) in improving stallion semen preservation by investigating sperm quality, redox balance, semen bacteriology, apoptosis, ultrastructure, and acrosome status of chilled stallion semen preserved at 4 °C for 72 h. This in vitro experiment was evidenced by molecular docking analysis. Twenty-five ejaculates from five stallions were collected and extended with 0 (BH...
Willems T, Dias ABM, Dedecker L, Matamalas SS, De Lange L, de Beauregard T.Nephrosplenic space entrapment (NSE) has reported recurrence rates of 8%‒23%, and closure of the nephrosplenic space (NSS) has been shown to lower the incidence. Described techniques for nephrosplenic space closure (NSC) with concurrent left dorsal displacement (LDD) of the colon at the time of surgery consist of reducing the displaced colon prior to closure. Methods: A retrospective study was performed to evaluate the medical records of horse undergoing NSC between 2017 and 2024. A total of 18 horses with LDD at time of NSC and 21 without displacement at time of NSC were evaluated. Short- a...
Curry O, Everett A, Pearson G, Dwyer C, Duncan J.Despite the availability of several equine pain assessment tools, their use in equine veterinary practice appears limited compared to small animal practice. This study explores potential barriers to equine pain assessment, as reported by staff at a single UK equine teaching hospital. Methods: Nine hospital staff members were interviewed using semi-structured interviews. Key themes were identified through reflexive thematic analysis. Results: Participants acknowledged the importance of pain assessment, yet highlighted limitations in current methods and their inconsistent use. Key challenges inc...
de Oliveira UV, Waap H, Gomes J, da Silva AN, Lacerda LC, da Silva Oliveira Costa T, de Sousa Lima T, Schares G, Pinheiro AM, Gondim LFP, Munhoz AD.The study aimed to assess the prevalence of horses reactive to Sarcocystis neurona, Neospora caninum, and Besnoitia besnoiti in horses from the state of Rio Grande do Sul, Brazil. Sera reactivity was tested by the Immunofluorescent Antibody Test (IFAT) in 354 equids sampled at slaughter, using a cut-off of 1:80 for S. neurona and 1:50 for N. caninum and B. besnoiti. Detection of N. caninum was attempted by PCR and bioassay in gerbils (Meriones unguiculatus) using brain samples collected from seropositive horses. Sera from 158 horses (44.63 %) (158/354; CI: 39.54-49.84 %) reacted to S. neuron...
Otero JC, Neild DM, Ferrante AA, Gambarotta MC, Caldevilla ML.Glycerol, a penetrating cryoprotectant, is most employed for deep freezing spermatozoa. However, it can induce toxic, chemical, and osmotic effects, altering the lipid structure of the sperm membrane. Rapid glycerol addition has been demonstrated to have fewer toxic effects than its removal. Objective: To minimize both the toxic and osmotic effects of glycerol on equine sperm through post-thaw dilution using two different extenders. Methods: Samples of equine semen frozen with 5% glycerol from nine stallions were thawed, re-diluted 1:2, and incubated for 30 minutes at 22°C in Tyrodes (Tyr) an...
Graide H, Duysens J, Frank T, Mouithys-Mickalad A, Niesten A, Sandersen C, Ceusters J, Serteyn D.Musculoskeletal pathologies present challenges in athletic horses, often leading to functional impairment. The slow or limited regenerative capacity of bone, joint, and tendon/ligament injuries, coupled with the limitations of conventional treatments, highlights the need for innovative therapies such as ortho-biologics and mesenchymal stem/stroma cells. Traditional 2D cell culture systems with fetal bovine serum (FBS) fail to replicate the complexity of the in vivo environment, whereas 3D cultures more accurately mimic native tissue architecture and cell-cell interactions. This study describes...
