Veterinary science and horses encompass the study and application of medical, surgical, and therapeutic practices to maintain and improve the health and welfare of equines. This field addresses a wide range of topics, including disease prevention, diagnosis, and treatment, as well as nutrition, reproduction, and behavior. Research in veterinary science for horses often involves understanding the pathophysiology of equine-specific diseases, developing advanced diagnostic techniques, and improving treatment protocols. This page gathers peer-reviewed research studies and scholarly articles that explore various aspects of veterinary science related to horses, providing insights into the latest advancements and methodologies in equine healthcare.
Bochnia M, Goetz F, Wensch-Dorendorf M, Koelln M, Zeyner A.Pelleted feeds (PF) are popular in horse nutrition because of high palatability and improved feeding hygiene, but ingestion is faster for PF than for cereals or muesli feed. The aim of the study was to evaluate whether variable amounts of two PFs produced with different physical properties from the same batch of feed can affect feed intake patterns in horses. Chewing patterns were measured in six warmblood mares (519 ± 36.3 kg) on two PFs (small-sized PF1: ø 5 mm, length 21.9 ± 4.97 mm, large-sized PF2: edge length 15.6 ± 0.14 × 15.6 ± 0.08 mm, length 54.4 ...
The typical vertebrate centromeres contain long stretches of highly repeated DNA sequences (satellite DNA). We previously demonstrated that the karyotypes of the species belonging to the genus are characterized by the presence of satellite-free and satellite-based centromeres and represent a unique biological model for the study of centromere organization and behavior. Using horse primary fibroblasts cultured in vitro, we compared the segregation fidelity of chromosome 11, whose centromere is satellite-free, with that of chromosome 13, which has similar size and a centromere containing long s...
Yokomori T, Tozaki T, Mita H, Miyake T, Kakoi H, Kobayashi Y, Kusano K, Itou T.According to oral traditions of horse caretakers and trainers, the differences in the position and number of facial hair whorls may be associated with temperamental traits. Elucidating genetic background of facial hair whorls and its relationship to temperamental traits may promote more efficient breeding and maintenance of racehorses. In this study, we estimated heritabilities of the position and number of facial hair whorls in Japanese Thoroughbred horses. Results: The number of facial hair whorls varied from one to four and heritability estimate in 4024 Thoroughbred horses was low (h2= 0....
Maisonpierre IN, Sutton MA, Harris P, Menzies-Gow N, Weller R, Pfau T.Accelerometry is an accepted means of quantifying human physical activity. Quantitative physical activity tracking could be beneficial for studies into equine health and disease prevention, for example in relation to obesity management. Objective: Validate accelerometer use in grazing horses, determine between-day repeatability, and assess the effects of pasture size on time budget (i.e. duration in each activity category). Methods: Proof of concept. Methods: Accelerometers (ActiGraph) were positioned at the poll. Horses underwent 5 min of observed activity in three categories: standing, graz...
Butler D, Valenchon M, Annan R, Whay HR, Mullan S.The purpose of this paper is to highlight some of the key challenges to racehorse welfare as perceived by racing industry stakeholders. The paper draws upon statements and transcripts from 10 focus group discussions with 42 participants who were taking part in a larger study investigating stakeholders' perceptions of racehorse welfare, which participants recognised as maintaining the physical and mental well-being of a performance animal. Analysis of the 68 statements participants identified as challenges produced nine themes. Among these, 26% (18 statements) of the challenges were health rela...
May-Davis S, Vermeulen Z, Brown WY.The 55-million-year history of equine phylogeny has been well-documented from the skeletal record; however, this is less true for the soft tissue structures that are now vestigial in modern horse. A recent study reported that two ligamentous structures resembling functional interosseous muscle II and IV were evident in Dutch Konik horses. The current study investigates this finding and compares it to members of the genus Equus to identify either a breed anomaly or functional primitive trait. Distal limbs (n = 574) were dissected from four species of Equus; E. caballus, E. asinus, E. przewalski...
Munhoz AD, Souza MA, Costa SCL, Freitas JS, Silva AND, Lacerda LC, Cruz RDS, Albuquerque GR, Pereira MJS.The aim of this study was to determine the frequency and factors associated with Toxoplasma gondii in naturally infected equids in northeastern Brazil. Serum samples from 569 equids (528 horses, 8 mules and 33 donkeys) were subjected to the indirect fluorescent antibody test. Generalized linear models were used to evaluate associated factors. Among the 569 animals sampled, 118 (30.6%) living in rural areas and 14 (26.42%) in urban areas were seropositive (p>0.05). Seropositive animals were observed on 95% of the farms and in all the municipalities. Donkeys/mules as the host, presence of dom...
Claußen G, Grau D, Hessel EF.It has been known for some years now that the occurrence of lameness in horses is closely related to the characteristics of the riding surface and that respiratory tract diseases can be induced by airborne particles. To ensure both optimal rideability (e.g., grip, elasticity, etc.) and low dust production, riding surfaces have to be regularly attended to, which also includes watering the floor. However, at present, it is not known what the individual moisture content of the various types of riding surface should be to ensure optimal rideability or what their potential for releasing dust is at ...
Sears KP, Kappmeyer LS, Wise LN, Silva M, Ueti MW, White S, Reif KE, Knowles DP.Theileria equi infection, exotic to the United States has reemerged through intravenous (iatrogenic) and tick-borne transmission. Surveillance at the US-Mexico border identified a new species, Theileria haneyi, (T. haneyi) (EP = Eagle Pass, Texas) which warranted additional investigation due to inability to detect by PCR targeting of T. equi ema-1 and EMA-1-cELISA validated for T. equi. Infection dynamics of T. haneyi were evaluated, including ability to superinfect in the presence of T. equi-Texas (T. equi), the isolate responsible for the reemergence of T. equi in the U S. Experimental i...
García EDA, Khusro A, Pacheco EBF, Adegbeye MJ, Barbabosa-Pliego A, Lagunas BC, Salas JMC, Mateos RG, Aarti C, Elghandour MMMY.The present context was designed to investigate the efficacy of devil fish (DF; Plecostomus sp.) silage and Staphylococcus saprophyticus on fermentation characteristics as well as greenhouse gases production mitigation attributes in horses. Four levels of ensiled DF at 0 (control DF0), 6 (DF6), 12 (DF12), and 18 (DF18) % were added into the diet. Moreover, three doses of S. saprophyticus (0, 1, and 3 mL/g dry matter [DM]) were used for in vitro fecal fermentation. The use of ensiled DF resulted in increased (P < .0001) pH during fermentation. The asymptotic gas production was the highest (P...
Witkowska-Piłaszewicz O, Bąska P, Czopowicz M, Żmigrodzka M, Szczepaniak J, Szarska E, Winnicka A, Cywińska A.Sport training leads to adaptation to physical effort that is reflected by the changes in blood parameters. In equine endurance athletes, blood testing is accepted as a support in training, however, only the changes before versus after exercise in creatine phosphokinase activity (CPK) and basic blood parameters are usually measured. This study is the first longitudinal investigation of the changes in routinely measured blood parameters and, additionally, serum amyloid A (SAA), during seven months, in Arabian horses introduced to endurance training and competing in events for young horses. It h...
Shakarami MH, Mohammadabadi T, Motamedi H, Sari M, Teimouri Yansari A.This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS). Results: Fresh faeces were collected from four Arabian horses. The cellulose-hydrolytic bacteria were isolated by using a medium amended with carboxymethyl cellulose (CMC). The activity of CMC was determined by measuring the release of reducing sugars from CMC. Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity. 16S rRNA sequence analysis showed that these isolat...
Piccolo L, Kienapfel K.Too much rein tension while riding may compromise the welfare of the horse. But who generates the tension on the reins-the horse or the rider? The primary aim of this pilot study was to evaluate the maximum rein tension that horses voluntarily maintain without a rider compared to rein tension with a rider. A secondary aim was to evaluate conflict behaviours in relation to rein tension. Thirteen horses were used, all fitted with customised "Animon" rein tension sensors (25 Hz, up to 600 N range), free-moving with side reins set in dressage competition frame with the noseline on the vertical. Re...
Moser K, Banse H.The objectives of this study were to compare the insulin and glucose responses of horses to 2 formulations of corn syrup, [Karo Light (Karo) available in the United States, and Crown Lily White (Crown), available in Canada]. Horses were evaluated under both fed ( = 14) and fasted ( = 10) conditions. Using a randomized crossover design, each horse underwent an oral sugar test using Karo or Crown syrup. There were no significant differences in insulin or glucose time of maximum concentration (T), maximum concentration (C), or area under the curve (AUC) or in insulin or glucose concentrations at ...
Stegelmeier BL, Colegate SM, Knoppel EL, Rood KA, Collett MG.Wild parsnip (Pastinaca sativa) has been associated with livestock and human photosensitization. An investigation of a natural occurrence of photosensitization of grazing horses identified wild parsnip as a possible cause. HPLC-MS and MS/MS analysis of this plant identified five furanocoumarins i.e., xanthotoxin, bergapten, isopimpinellin, imperatorin and a putative methoxyimperatorin. Goats fed this wild parsnip were largely unaffected. Xanthotoxin was not detected in the serum of parsnip-fed goats or in the serum of goats dosed orally or intravenous with purified xanthotoxin. Cutaneous appli...
Łuszczyński J, Pieszka M, Petrych W, Stefaniuk-Szmukier M.The structure and changes occurring to horses' teeth during ontogeny are not only used to assess the degree of somatic maturity but also the development of universal patterns and is therefore used to determine the age of horses. Research shows that methods of assessing the age of horses based on the appearance of teeth tend to suffer from relatively large errors. This is probably influenced by the results of intensive selection and being kept in living conditions that differ substantially from their natural environment. This study aimed to assess the suitability of selected features of the inc...
Schanz L, Krueger K, Hintze S.Identifying valid indicators to assess animals' emotional states is a critical objective of animal welfare science. In horses, eye wrinkles above the eyeball have been shown to be affected by pain and other emotional states. From other species we know that individual characteristics, e.g., age in humans, affect facial wrinkles, but it has not yet been investigated whether eye wrinkle expression in horses is systematically affected by such characteristics. Therefore, the aim of this study was to assess how age, sex, breed type, body condition, and coat colour affect the expression and/or the as...
Mendonça T, Bienboire-Frosini C, Kowalczyk I, Leclercq J, Arroub S, Pageat P.The learning and cognitive challenges that horses may face differ according to the activities in which they are involved. The aim of this investigation was to study the influence of equine activities on the behavioral responses and autonomic nervous system (ANS) activity of adult horses. Forty-one horses were divided into four groups: dressage (9), jumping (10), eventing (13) and equine-assisted activity/therapy (9). A test was created to compare the horses' behavioral and physiological responses to different stimuli. The goal was always to obtain a treat. To study the ANS activity, heart rate...
Jansen S, Heitmann A, Lühken R, Leggewie M, Helms M, Badusche M, Rossini G, Schmidt-Chanasit J, Tannich E.The continuous circulation of West Nile virus (WNV) in Central, South and East Europe and its recent detection in several dead birds and two horses in Germany highlights the need for information on WNV vector competence of mosquitoes from Central Europe. Therefore, three common Culex species (Culex pipiens biotype pipiens, Culex pipiens biotype molestus and Culex torrentium) from Germany were orally infected with WNV and kept at 18 °C, 21 °C, 24 °C or 27 °C for 14 or 21 days post infection (dpi). Thereafter viable WNV was present in the saliva in all tested taxa, but only at incubation tem...
McCoy AM, Beeson SK, Rubin CJ, Andersson L, Caputo P, Lykkjen S, Moore A, Piercy RJ, Mickelson JR, McCue ME.Several horse breeds have been specifically selected for the ability to exhibit alternative patterns of locomotion, or gaits. A premature stop codon in the gene DMRT3 is permissive for "gaitedness" across breeds. However, this mutation is nearly fixed in both American Standardbred trotters and pacers, which perform a diagonal and lateral gait, respectively, during harness racing. This suggests that modifying alleles must influence the preferred gait at racing speeds in these populations. A genome-wide association analysis for the ability to pace was performed in 542 Standardbred horses (n = 17...
Mendonça RS, Ringhofer M, Pinto P, Inoue S, Hirata S.In the rapidly expanding field of comparative thanatology, reports from a wide range of taxa suggest that some aspects of a concept of death may be shared by many non-human species. In horses, there are only a few anecdotal reports on behaviors toward dead conspecifics, mostly concerning domestic individuals. Here, we describe the case of a 2-month-old, free-ranging male foal that died around 12 h after being found severely injured due to a presumed wolf attack, focusing on other individuals' reactions to the dying foal. We also placed camera traps near horse carcasses to investigate reaction...
Yadav SC, Kumar P, Khurana S, Kumar R.Trypanosomosis caused by Trypanosoma evansi, commonly known as "surra," is a wasting disease affecting equids, camels, cattle, as well as several other domestic and wild animals. No systematic information is available on disease pattern in equines for development of control and treatment strategies in endemic areas in India. The present study was undertaken with a comprehensive plan to screen large population of equines using indirect enzyme-linked immunosorbent assay to obtain epidemiological information on "surra" in different agro-climatic zones of India. A total of 6455 serum samples were ...
Quaresma M, Bacellar D, Leiva B, Silva SR.The use of the measurement of heart girth (HG), in locations where a scale is not available, with the application of weight estimation formulas or special weight tapes, is well established as a practical and accurate way to estimate the live weight (LW). Although several studies were performed to correlate donkey body measurements and LW, none of these was done in the large frame European donkey breeds. When using smaller frame breeds formulas, the tendency was to underestimate the live weight of larger frame breeds. The sample used in this study consisted of 65 Miranda breed donkeys, with age...
Catalano DN, Heins BJ, Missaghi S, Hathaway MR, Martinson KL.Goldfish (Carassius auratus) have been reported as a method to keep water tanks clean; however, little information exists on this approach. The objectives were to evaluate the efficacy of goldfish on maintaining water quality in tanks and to evaluate the frequency that this method is used. The first objective was completed during June through October 2017 in St. Paul, MN, using plastic and metal 379 L stock tanks, each with and without goldfish in a drylot that housed six adult horses. The stocking rate was 5 goldfish per tank. Daily readings of total dissolved solids (TDS) and water turbidit...
Casenave P, Leclere M, Beauchamp G, Blais MC.After-hours or out-of-clinic crossmatches are often limited by the lack of access to specialized material and technical expertise. Objective: The goal was to adapt a stall-side crossmatch test for pretransfusion evaluation in horses. Methods: Twelve healthy mares (plasma and blood donors, teaching mares). Methods: In a prospective study, blood from 12 mares was used to compare the results of 132 crossmatches performed with a rapid gel assay to crossmatches performed with a microgel column assay, and with predicted compatibilities based on blood types and detection of antibodies at a reference ...
Stojiljkovic N, Leroux F, Bubanj S, Popot MA, Paris A, Tabet JC, Junot C.There is an urgent need to implement holistic and untargeted doping control protocols with improved discriminatory power, compared to conventional methods that only target doping agents. Metabolomics, which aims to characterize all metabolites present in biological matrices, could fulfill this need. In this context, the aim of this study was to evaluate the impact of environmental factors on the ability to obtain a metabolic signature of stanozolol administration in horse doping situation. Urine samples from 16 horses breeded in two different places were collected over a one-year period, befor...
Grimes JA, Wallace ML, Schmiedt CW, Parks AH.To compare surgical models for teaching enterotomies to students. Methods: Prospective, randomized study. Methods: Second-year veterinary students (n = 59) and faculty surgeons/surgery residents (n = 19). Methods: Participants performed an enterotomy on each of 3 models (equine cadaver intestine, SurgiReal small intestine simulator, and SynDaver canine bowel) and completed a survey comparing them to either an enterotomy on an anesthetized pig (students) or intestinal surgery experience (faculty/residents). Surveys results were compiled and analyzed. Results: Both student and faculty/resident g...
Brøkner C, Austbø D, Næsset JA, Blache D, Bach Knudsen KE, Tauson AH.The hypothesis for this study was that a higher dietary proportion of soluble fibre would result in stable and constant plasma metabolite and regulatory hormone concentrations. The study was a 4×4 Latin Square design with a sequence of 17 days adaptation to the ration followed by 8 sampling days. The feed rations consisted of only timothy hay (H), hay plus molassed sugar beet pulp combined with either whole oats (OB) or barley (BB) and hay plus a loose chaff-based concentrate (M). Four horses were fitted with permanent caecal cannulas and liquid caecal content was withdrawn manually and blood...
Stegelmeier BL, Colegate SM, Knoppel EL, Rood KA, Collett MG.Wild parsnip (Pastinaca sativa) has been associated with livestock and human photosensitization. An investigation of a natural occurrence of photosensitization of grazing horses identified wild parsnip as a possible cause. HPLC-MS and MS/MS analysis of this plant identified five furanocoumarins i.e., xanthotoxin, bergapten, isopimpinellin, imperatorin and a putative methoxyimperatorin. Goats fed this wild parsnip were largely unaffected. Xanthotoxin was not detected in the serum of parsnip-fed goats or in the serum of goats dosed orally or intravenous with purified xanthotoxin. Cutaneous appli...
Pozharskiy A, Abdrakhmanova A, Beishova I, Shamshidin A, Nametov A, Ulyanova T, Bekova G, Kikebayev N, Kovalchuk A, Ulyanov V, Turabayev A....Horses are traditionally used in Kazakhstan as a source of food and as working and saddle animals as well. Here, for the first time, microarray-based medium-density single nucleotide polymorphism (SNP) genotyping of six traditionally defined types and breeds of indigenous Kazakh horses was conducted to reveal their genetic structure and find markers associated with animal size and weight. The results showed that the predefined separation between breeds and sampled populations was not supported by the molecular data. The lack of genetic variation between breeds and populations was revealed by t...
Conley AJ, Ball BA.Historically, studies on the endocrinology of pregnancy and parturition in horses have made major contributions of relevance to mammals in general. Recent use of liquid chromatography mass spectrometry, measuring multiple steroid hormones simultaneously in blood, foetal and placental tissues throughout normal gestation, and in mares with experimentally induced placentitis, has advanced our current understanding of many of the unusual strategies seen during gestation and at foaling. This includes the stimulation of luteal steroidogeneisis by equine chorionic gonadotropin (eCG) from the endometr...
Quinn GC, Kidd JA, Lane JG.Previous studies of sinus surgery on standing horses have been based on techniques which utilise powered hand bone saws and preserve the bone flap, which was thought to be essential for a good cosmetic result. This report describes a simplified technique applied to the standing horse where the sinus flap construction used a large skull trephine and where the bone flap is necessarily discarded. Objective: To assess whether the modified standing frontonasal flap (SFF) surgery offers an effective method to investigate and/or treat sinunasal disorders in the horse. Methods: The case records of 60 ...
Jäderkvist Fegraeus K, Hirschberg I, Árnason T, Andersson L, Velie BD, Andersson LS, Lindgren G.The Icelandic horse is a breed known mainly for its ability to perform the ambling four-beat gait 'tölt' and the lateral two-beat gait pace. The natural ability of the breed to perform these alternative gaits is highly desired by breeders. Therefore, the discovery that a nonsense mutation (C>A) in the DMRT3 gene was the main genetic factor for horses' ability to perform gaits in addition to walk, trot and canter was of great interest. Although several studies have demonstrated that homozygosity for the DMRT3 mutation is important for the ability to pace, only about 70% of the homozygous mutan...
Okabe K, Mukai K, Ohmura H, Takahashi T, Miyata H.We examined the effect of transient hypoxic exposure during high-intensity exercise on satellite cell activation and angiogenesis in Thoroughbred skeletal muscle. Methods: Six Thoroughbreds horses (6.3±0.8 years old) ran on a treadmill in normoxia (N; FIO2=21%) and hypoxia (H; FIO2=16%) at the same speed for the same duration, and oxygen consumption and plasma lactate and hemoglobin concentrations were measured. In addition, muscle biopsy samples were obtained from gluteus medius muscle before exercise and immediately, 4 hours, 24 hours, 3 days and 7 days after exercise, and immunohistochemic...
Nikitkina EV, Dementieva NV, Shcherbakov YS, Atroshchenko MM, Kudinov AA, Samoylov OI, Pozovnikova MV, Dysin AP, Krutikova AA, Musidray AA....The semen quality of stallions including sperm motility is an important target of selection as it has a high level of individual variability. However, effects of the molecular architecture of the genome on the mechanisms of sperm formation and their preservation after thawing have been poorly investigated. Here, we conducted a genome-wide association study (GWAS) for the sperm motility of cryopreserved semen in stallions of various breeds. Methods: Semen samples were collected from the stallions of 23 horse breeds. The following semen characteristics were examined: progressive motility (PM), p...
Grobbel M, Lübke-Becker A, Alesík E, Schwarz S, Wallmann J, Werckenthin C, Wieler LH.A total of 417 isolates of Escherichia coli collected from five animal species/organ system combinations from swine [urinary/genital tract (UGT) incl. mastitis metritis agalactia syndrome], horses [genital tract (GT)] and dogs/cats [respiratory tract (RT), UGT and gastrointestinal tract (GIT)] were analysed quantitatively for their susceptibility against different antimicrobial agents by determination of minimum inhibitory concentrations. Regardless of which animal species the strains originated from, resistance appeared most frequently against sulfamethoxazole (18-59%), tetracycline (14-54 %)...
Maharana BR, Potliya S, Ganguly A, Bisla RS, Mishra C, Ganguly I.Equine ocular setariasis arising mainly from ectopic infestation of Setaria digitata is a common vision impairing ophthalmic disease in India, and the identification of this filarial nematode is based solely on morphology. However, morphological characters alone are inadequate to detect and differentiate S. digitata from its congeners. The present communication reports the first phylogenetic characterization of equine S. digitata from India based on sequences derived from the mitochondrial cytochrome c oxidase subunit 1 (COI), the mitochondrial small subunit ribosomal DNA (12S rDNA), and the n...
Brown JJ, Thomson W, Clegg P, Eyre S, Kennedy LJ, Matthews J, Carter S, Ollier WE.The full extent of the polymorphism of ELA-DRA in Equidae is not yet known. Given the apparent differences in DRA polymorphisms between Equidae and other species, the aims of this study were to more fully characterize ELA-DRA, determine the extent of gene polymorphism and establish the allele-frequency distribution. An allele reference panel for the second exon of ELA-DRA was established by sequence-based typing of 69 equine DNA samples consisting of various breeds of domestic horse (Equus caballus), together with donkeys (Equus asinus), Grant's zebras (Equus boehmi) and one onager (Equus hemi...
Choi YH, Harding HD, Hartman DL, Obermiller AD, Kurosaka S, McLaughlin KJ, Hinrichs K.The reported patterns of trophectodermal expression of POU5F1 protein in blastocysts vary among species, and are possibly related to the differences in placental growth and function. This study investigated the pattern of embryonic POU5F1 expression in the horse, a species with delayed placental formation. Immature equine oocytes expressed POU5F1 protein in the cytoplasm and nucleus. Staining for POU5F1 protein in in vitro-produced (IVP) embryos decreased to day 5 of culture, then the nuclear staining increased to day 7. IVP day-7 to -11 blastocysts showed POU5F1 staining in nuclei throughout ...
Chandley AC, Short RV, Allen WR.A detailed investigation of testicular meiosis in a mule, a hinny and a Przewalski horse/domestic horse hybrid were made. Abnormalities of pairing were observed in the mule and hinny in most germ cells at the pachytene stage of meiotic prophase, and spermatogenesis was alsmot totally arrested. A few mature spermatozoa were recovered from the ejaculate and epididymal flushings of the hinny. The Przewalski horse/domestic horse hybrid was fertile and showed normal spermatogenesis. Chromosome banding studies showed a close homology between the karyotypes of the Prezwalski horse (Equus przewalskii,...
Ahrens E, Stranzinger G.Previous research revealed that the karyotypes of Equus przewalskii (2n = 66) and Equus caballus (2n = 64) differ by one pair of metacentric chromosomes, present in ECA but not in EPR, and two pairs of acrocentric chromosomes found only in the EPR karyotype. The formation of a trivalent during meiosis in a male F1 hybrid and the homologies in G-banding patterns suggest that ECA 5 corresponds to two acrocentric EPR chromosomes resulting from a Robertsonian fusion or fission event. Chromosomal investigations of a female interspecies F1 hybrid including banded karyograms and fluorescence in situ ...
Janczarek I, Stachurska A, Wilk I, Krakowski L, Przetacznik M, Zastrzeżyńska M, Kuna-Broniowska I.Touching the skin by gentle stroking is frequently used to reward horses. The objective of the study was to examine emotional excitability and behaviour of horses in response to stroking different regions of their bodies. The study included 15 adult warmbloods and 15 adult ponies, nine geldings and six mares within each type of horse. First, a novel-object test was conducted. For five successive days, one of five regions of the horse's body was stroked on each side for 5 min. Heart rate and heart rate variability were monitored at rest, during the test and stroking. Simultaneously, horse beha...
Lönnell AC, Bröjer J, Nostell K, Hernlund E, Roepstorff L, Tranquille CA, Murray RC, Oomen A, van Weeren R, Bitschnau C, Montavon S, Weishaupt MA....Training regimens of showjumping horses under field conditions are largely undocumented. Objective: The aims of this study were to quantify and compare training regimens used in professional-level showjumping yards, with respect to time exercised and type of activity. Methods: Prospective cohort study. Methods: A prospective 6-month cohort study of showjumping horses in 4 European countries (The Netherlands, Sweden, Switzerland, Great Britain) was designed to analyse training and health data, in yards with several horses in training and riders competing at professional level. Riders documented...
Ward MP.Since first being detected in New York in 1999, West Nile virus (WNV) has spread throughout the United States and more than 20,000 cases of equine WNV encephalomyelitis have been reported. A spatial model of disease occurrence was developed, using data from an outbreak of serologically confirmed disease in an unvaccinated population of horses at 108 locations in northern Indiana between 3 August and 17 October 2002. Daily maximum temperature data were recorded at meteorological stations surrounding the study area. The distribution of the total number of degree-days elapsing between July 4 and ...
Hammond SA, Horohov D, Montelaro RC.Naive T cells can be activated both in vivo and in vitro by specialized antigen presenting cells, dendritic cells (DC), with potent antigen-specific, immunostimulatory activity. Indeed, DC can provide an extremely powerful and important immunological tool by which to potentiate the immune response for specific recognition of foreign antigens. Until recently, the direct isolation of DC from PBMC required laborious procedures with extremely poor yields (<0.1%). Methods have been developed for the human, lower primate, and murine model systems to propagate large numbers of DC from PBMC or bone...
Osborne RL, Coggins MK, Walla M, Dawson JH.The heme-containing respiratory protein, myoglobin (Mb), best known for oxygen storage, can exhibit peroxidase-like activity under conditions of oxidative stress. Under such circumstances, the initially formed ferric state can react with H2O2 (or other peroxides) to generate a long-lived ferryl [Fe(IV)=O] Compound II (Cpd II) heme intermediate that is capable of oxidizing a variety of biomolecules. In this study, the ability of Mb Cpd II to catalyze the oxidation of carcinogenic halophenols is demonstrated. Specifically, 2,4,6-trichlorophenol (TCP) is converted to 2,6-dichloro-1,4-benzoquinone...
Muñoz A, Riber C, Santisteban R, Lucas RG, Castejón FM.Metabolic responses to exercise differ between Andalusian horses and other breeds, although changes in plasma muscle enzymes have not been reported and most useful information is obtained from animals subjected to different training programmes. The objectives of this study were to 1) describe the changes in plasma enzymes during exercise in different horse breeds in relation to other biochemical parameters (Experiment A) and 2) assess the effect of training duration on these measures (Experiment B). Twenty stallions, 9 Andalusian (AN), 7 Arabian (A) and 4 Anglo-Arabian (AA), age 5-10 years, we...
Cope SE, Catts EP.Parahost behavior of adult Gasterophilus intestinalis (DeGeer) was observed during the summers of 1978 and 1979 near pastured horses on two farms near Newark, Del. Adult fly occurrence, although sparse in early summer, indicated a gradual increase, with three surges in emergence. In the laboratory, males lived 1-3 d and females 1-2 d. In the field, marked flies were not seen on days following their release. Adult behavior indicated an urgency in mating and oviposition dictated by a short life span. Male flight patterns were characterized by in-flight searches for females and intraspecific conf...
Quigley A, Sekiya M, Egan S, Wolfe A, Negredo C, Mulcahy G.There is little information on the prevalence of Fasciola hepatica infection in the horse population in Ireland or the potential impact of fluke infection on animal health. Objective: To investigate F. hepatica infection in the Irish horse population and to assess the diagnostic potential of an indirect enzyme-linked immunosorbent assay (ELISA) based on the F. hepatica recombinant cathepsin L1 (CL1) antigen. Methods: Cross-sectional abattoir survey of horses for liver fluke status. Methods: Animals (n = 200) were examined at an abattoir between May 2013 and April 2014. Horses were graded ante ...
Lucassen A, Hankel J, Finkler-Schade C, Osbelt L, Strowig T, Visscher C, Schuberth HJ.Feed supplements such as fermentation products (SCFP) alter immune responses in horses. The purpose of this study was to analyze whether a prebiotic activity of the SCFP alters the gut microbiome in horses. Racehorses were fed either SCFP (Olimond BB, OLI, = 6) or placebo pellets (PLA, = 5) for 43 days. Fecal microbiota analysis was performed using 16S rRNA gene sequencing. The numbers and function of circulating immune cell subpopulations were analyzed by flow cytometry. SCFP supplementation resulted in non-consistent differences in fecal microbiota between the PLA and OLI during the feedi...
Westermann CM, Dorland L, Wijnberg ID, de Sain-van der Velden MGM, van Breda E, Barneveld A, de Graaf-Roelfsema E, Keizer HA, van der Kolk JH.The objective of this study is to assess the influence of acute exercise, training and intensified training on the plasma amino acid profile. In a 32-week longitudinal study using 10 Standardbred horses, training was divided into four phases, including a phase of intensified training for five horses. At the end of each phase, a standardized exercise test, SET, was performed. Plasma amino acid concentrations before and after each SET were measured. Training significantly reduced mean plasma aspartic acid concentration, whereas exercise significantly increased the plasma concentrations of alanin...
The Journal of heredityNovember 8, 2013
Volume 105, Issue 2 163-172 doi: 10.1093/jhered/est075
McCoy AM, Schaefer R, Petersen JL, Morrell PL, Slamka MA, Mickelson JR, Valberg SJ, McCue ME.A dominantly inherited gain-of-function mutation in the glycogen synthase (GYS1) gene, resulting in excess skeletal muscle glycogen, has been identified in more than 30 horse breeds. This mutation is associated with the disease Equine Polysaccharide Storage Myopathy Type 1, yet persists at high frequency in some breeds. Under historical conditions of daily work and limited feed, excess muscle glycogen may have been advantageous, driving the increase in frequency of this allele. Fine-scale DNA sequencing in 80 horses and genotype assays in 279 horses revealed a paucity of haplotypes carrying th...
Rivera D, Allel K, Dueñas F, Tardone R, Soza P, Hamilton-West C, Moreno-Switt AI.Salmonella is a major bacterial foodborne pathogen that causes the majority of worldwide food-related outbreaks and hospitalizations. Salmonellosis outbreaks can be caused by multidrug-resistant (MDR) strains, emphasizing the importance of maintaining public health and safer food production. Nevertheless, the drivers of MDR Salmonella serovars have remained poorly understood. In this study, we compare the resistance profiles of Salmonella strains isolated from 4047 samples from domestic and wild animals in Chile. A total of 106 Salmonella strains (2.61%) are isolated, and their serogroups are ...
Khumsap S, Clayton HM, Lanovaz JL, Bouchey M.A database of biomechanical variables obtained from normal horses walking at a range of velocities is needed for comparison with the variables obtained from lame horses in which velocity cannot be predetermined. The objective was to investigate velocity-dependent changes in selected kinematic variables, ground reaction forces (GRF) and net joint energies in the forelimb and to develop statistical equations to calculate expected values of these variables for horses walking at different velocities. Five sound horses walked at a range of velocities (0.82 to 1.91 m/s) over a force plate. Kinematic...
Mazhitova AT, Kulmyrzaev AA.This study was carried out to determine the influence of milking season on amino acid (AA) profile and chemical composition of milk samples from Kyrgyz native breed mares under traditional pasture conditions. Milk samples were collected monthly from May to August 2014 from mares grazing at 2,200 m above sea level. The AA composition was determined by precolumn derivatization with diethyl ethoxymethylenemalonate in HPLC and 18 AA were determined in mare milk. The analytical parameters of linearity (0.01-4 μg/mL), precision of the method (0.26-4.88% relative standard deviation), derivatization ...
Ryan EG, Beatty SH, Gray E, Field N, Liston R, Rhodes V, Donlon J.Retention of veterinary practitioners has arisen as a significant problem in recent years in Ireland. No prior Irish peer-reviewed publications have addressed this problem. An online questionnaire was available through social media and via email to Irish vets from January to November 2019. The aim of this survey was to ascertain the factors contributing to the problem of vet retention in Ireland. Results: A total of 370 eligible responses were received. The median age of respondents was 31 and the gender balance was 250 females (68%) to 118 males (32%). The majority of respondents worked in cl...
Palmgren Karlsson C, Jansson A, Essén-Gustavsson B, Lindberg JE.The aim of this study was to investigate the effect of partly replacing oats with molassed sugar beet pulp in a traditional hay/oat diet on nutrient utilisation and metabolic parameters in exercising horses. In a change-over experiment, 4 Standardbred geldings were fed a hay and oat-based diet (Oat diet) and a hay and oat-based diet where oats was partially replaced with molassed sugar beet pulp (MSBP diet). Each experimental period was 21 days during which total collection of faeces and urine was made and an exercise test (ET) performed. The crude fat digestibility was lower on the MSBP diet ...
