Analyze Diet

Journal of equine veterinary science.

Periodical
Veterinary Medicine
Horses
Publisher:
William E. Jones,. New York : Elsevier (2003)
Frequency: Monthly
Country: United States
Language: English
Start Year:1981 -
ISSN:
0737-0806 (Print)
0737-0806 (Linking)
Impact Factor
1.3
2022
NLM ID:8216840
(DNLM):J20290000(s)
(OCoLC):07643410
Classification:W1 JO644CD
Equine-based community service offered through horse breed association youth programs: Parent and community partner perceptions of participation, benefits, and program growth.
Journal of equine veterinary science    September 10, 2026   106142 doi: 10.1016/j.jevs.2026.106142
Culwell R, Nicodemus MC, Jousan D, Irvin L, Williams T, Hall B.Equine-based community service activities offered through horse breed association youth programs provide opportunities for youth to engage in volunteerism while interacting with horses. Although these programs are widely available, research examining stakeholder perceptions remains limited. Objective: To examine parent and community partner perceptions of equine-based community service activities offered through horse breed association youth programs, including perceived benefits, participation patterns, recruitment, implementation challenges, and opportunities for program development. Methods...
Limited evidence for exposure of horses in the United Kingdom to influenza D virus.
Journal of equine veterinary science    September 10, 2026   Volume 167 106141 doi: 10.1016/j.jevs.2026.106141
Wu G, Norman F, Maina MM, Kuchipudi SV, Nissly RH, Scott SD, Temperton N, Dunham SP, Daly JM.Since it was first detected in 2011, antibodies to influenza D virus (IDV) have been found in several species, although cattle are the major host. In cattle, IDV is typically associated with mild respiratory signs, but it is clinically significant as a component of bovine respiratory disease complex. Although antibodies to IDV have been detected in equids, no association of IDV with naturally occurring respiratory disease in horses has yet been demonstrated. Objective: The aim of this study was to determine whether IDV may be a causal agent of equine respiratory disease in the UK. Methods: Nas...
Effects of steaming and storage on microbial quality and nutrient losses of hay.
Journal of equine veterinary science    September 10, 2026   Volume 167 106146 doi: 10.1016/j.jevs.2026.106146
Kummer LK, Schrödl W, Schnabel CL, Schwarzenbolz U, Vervuert I, Lohmann KL.Steaming reduces microbial content of hay but the effect of steaming on nutrient quality and the effects of storage after steaming on microbial content are incompletely known. Objective: It was hypothesized that steamed hay can be stored for up to 24 h without an increase in microbial content and that steaming of hay favors the increase in measurable Maillard reaction products. Methods: Spoiled hay was steamed and then stored in hay nets, buckets or the hay steamer itself under summer and winter climate conditions. Microbial content was assessed by bacterial and fungal culture before and after...
Anesthetic Recovery Following a Midazolam-Containing Co-Induction Protocol in Horses Undergoing Total Intravenous Anesthesia.
Journal of equine veterinary science    September 9, 2026   106144 doi: 10.1016/j.jevs.2026.106144
Claudino JA, Avelar PHS, Rosa MCB, Ferrante M, Souza GP.Anesthetic recovery is a critical phase in horses under field conditions, and induction protocols may influence recovery quality during total intravenous anesthesia. Objective: To compare two anesthetic induction protocols, one including midazolam and the other based solely on xylazine and ketamine, regarding anesthetic induction and recovery quality, total recovery time, and attempts to achieve standing in horses undergoing total intravenous anesthesia. Methods: Twelve healthy male horses older than 2 years were randomly assigned to XC (xylazine 1 mg/kg and ketamine 2 mg/kg) or XCM (xylazine ...
Exhaled breath condensate in horses: a first microbiological insight.
Journal of equine veterinary science    September 8, 2026   106139 doi: 10.1016/j.jevs.2026.106139
Santi TF, Nogara MF, Valente MLVM, Weber SH, Oliveira TM, Bogossian PM, Michelotto PV.Exhaled breath condensate (EBC) is a non-invasive method for obtaining samples reflecting the airway lining fluid; however, its microbiological composition remains poorly characterized. Objective: To perform an exploratory microbiological comparison of EBC in clinically healthy horses maintained under different environmental conditions. Methods: An observational study included 30 mares equally distributed into two groups: horses housed in stalls (Racetrack group) and horses maintained under pasture conditions (Farm group). EBC samples were collected using a validated device developed by the re...
Training- and competition-related changes on circulating adrenocorticotropic hormone and cortisol response in Thoroughbred Racehorses.
Journal of equine veterinary science    September 4, 2026   106137 doi: 10.1016/j.jevs.2026.106137
Cravana C, Fazio E, Satué K, Brancato G, Deborah F.Exercise is a physiological challenge inducing intensity-dependent hypothalamic-pituitary-adrenal (HPA) axis responses. The hypothesis was that training and racing sessions induce different HPA axis activation. Objective: To assess whether different exercise sessions affect equine adrenal responsiveness according to age and sex. Methods: Ten clinically healthy Thoroughbred racehorses (6 females and 4 males) were recruited. Four were 2 years old and six were 3 years old. Horses were subjected to two exercise conditions: training and competitive racing sessions. Blood samples were collected at r...
Hidden Tarsal Lesions: The Role of Flexed and Oblique Radiographs in Young Horses.
Journal of equine veterinary science    August 28, 2026   106136 doi: 10.1016/j.jevs.2026.106136
Cianci JM, Dobbs EC, Bills KW, Engiles JB, Orsini JA.Radiographically apparent tarsal lesions associated with developmental orthopedic disease and joint sepsis are common in young horses. Standard radiographic projections are routinely used to identify osseous changes but may fail to detect lesions due to superimposition and complex anatomy. This retrospective case series describes plantar trochlear ridge lesions in young horses that were not identified on standard four projection radiograph series but were detected using flexed lateromedial and flexed oblique projections. Cases were selected based on persistent clinical signs localizing to the ...
No evidence of H5N1 avian influenza virus infection in Japanese horses in 2025.
Journal of equine veterinary science    August 26, 2026   Volume 167 106131 doi: 10.1016/j.jevs.2026.106131
Kawanishi N, Hiono T, Kambayashi Y, Bannai H, Tsujimura K, Yamanaka T, Isoda N, Sakoda Y, Nemoto M.The global spillover of clade 2.3.4.4b H5N1 highly pathogenic avian influenza virus into mammalian hosts has become a concern for public health as well as for the equine industry. However, previous reports on H5N1 natural infection and the presence of specific antibodies in horses remain limited. Therefore, we conducted a serosurveillance study to assess the prevalence of H5N1 antibodies in serum samples collected from 400 horses in Japan. Samples were screened using competitive ELISA (cELISA) and hemagglutination inhibition (HI) tests against H5-recombinant protein and H5N1 viral antigen, res...
Walking prior to contrast tenogram radiograph acquisition improves radiological identification of equine digital flexor tendon sheath pathology.
Journal of equine veterinary science    August 25, 2026   106132 doi: 10.1016/j.jevs.2026.106132
Martynski P, Kent A, Bathe A.the protocol for mobilization of the fetlock region prior to acquiring a contrast tenogram radiograph is not clearly defined. Objective: to determine if walking exercise improves identification of DFTS pathology on contrast tenography. Methods: Prospective cohort study. Nineteen horses that underwent contrast tenography and subsequent surgical tenoscopy were included. A tenogram protocol was developed to enable comparison of diagnostic accuracy after increasing mobilization of the affected limb post-injection. Following contrast injection three tenogram radiographs were taken, (1) immediately ...
Intraoperative Mechanical Power and Post-Anesthetic Respiratory Outcomes in Horses: A Retrospective Cohort Study.
Journal of equine veterinary science    August 25, 2026   106135 doi: 10.1016/j.jevs.2026.106135
Lutvikadic I, Hopster K, Watkins AR.Mechanical power (MP) represents the ventilatory-delivered energy to the respiratory system, integrating multiple variables into a single parameter. It has been linked to ventilator-induced lung injury and post-ventilation complications in human medicine, while clinical relevance in equine anesthesia remains insufficient. Objective: To evaluate the association between intraoperative mechanical power and postoperative respiratory outcomes in horses undergoing inhalant anesthesia. Methods: This retrospective cohort study analyzed anesthesia records from 843 adult horses anesthetized between 2023...
Comparative genomics of Standardbred horses reveals candidate regions under selection for harness racing performance.
Journal of equine veterinary science    August 25, 2026   106133 doi: 10.1016/j.jevs.2026.106133
Harness racing performance in horses is a complex polygenic trait influenced by genetic background, breeding history, training, and environment. Understanding genomic patterns shaped by long-term artificial selection provides insights into performance-related traits and breed-specific genomic variation. Objective: This study aimed to characterize genomic differentiation, population structure, and candidate genomic regions potentially influenced by historical selection in Standardbred horses using comparative whole-genome sequencing involving diverse horse breeds. Methods: Whole-genome sequenci...
Survey of rider and horse size proportions and associated rider perceptions.
Journal of equine veterinary science    August 24, 2026   106130 doi: 10.1016/j.jevs.2026.106130
Karikoski NP, Hyytiäinen HK, Junnila JJT, Rossi HS.The influence of a rider's size on equine performance and welfare remains a topic of debate in the equestrian industry. Most previous research has examined the weight ratios of riders and horses in controlled experimental settings, and the existing perceptions of acceptable rider-horse unit proportions have not been investigated. Objective: The aim of this study was to investigate horse and rider size and skill proportions among Finnish horses and riders. The study also investigated riders' perceptions of the acceptability of these size proportions. Methods: Riders over 18 years of age were in...
Questionnaire-Based Investigation into Stallion Management Practices in 28 Countries.
Journal of equine veterinary science    August 14, 2026   106128 doi: 10.1016/j.jevs.2026.106128
de Oliveira RA, Aurich J, Aurich C.Modern stud farm practices may limit stallion social contact, with potential welfare and safety implications. Objective: To describe housing, management and safety outcomes for stallions kept at horse breeding operations throughout the world and to assess regional differences. Methods: An online questionnaire was distributed via mailing lists and WhatsApp; 116 complete responses from breeding operations were collected and analysed. The focus was on management of the breeding facility, and stallion welfare indicated by management, handling and housing. Results: Respondents were from Europe (40....
Wound healing of experimental cutaneous wounds in horses: comparison between a topical formulation containing powdered coconut water and an antimicrobial ointment.
Journal of equine veterinary science    August 13, 2026   106126 doi: 10.1016/j.jevs.2026.106126
Meneses CSB, Silva LDM, Cardoso JEC, Rodrigues FRN, Neto EMR.Skin wounds in horses often present prolonged healing, stimulating the search for alternative topical therapies. To date, there are no experimental studies in horses evaluating topical formulations containing powdered coconut water. Objective: To compare the early macroscopic and morphometric wound-healing outcomes and the histopathological findings at day 14 in experimental cutaneous wounds in horses treated with a topical formulation containing powdered coconut water versus a reference antimicrobial ointment. Methods: Eight clinically healthy horses received two standardized circular skin wo...
Effects of omeprazole therapy administered via different routes on urinary parameters and selected interleukins in horses with equine gastric ulcer syndrome.
Journal of equine veterinary science    August 13, 2026   106127 doi: 10.1016/j.jevs.2026.106127
Siwińska N, Żak-Bochenek A, Trodd H, Czekaj Z, Kumiega E, Niedźwiedź A, Hewetson M.Equine gastric ulcer syndrome (EGUS) is commonly treated with proton pump inhibitors such as omeprazole, administered orally or by injection. In humans, omeprazole has been associated with acute interstitial nephritis (AIN) and acute kidney injury (AKI), potentially linked to oxidative stress, and urinary interleukins may serve as early non-invasive markers. However, this association has not been established in horses. Objective: To evaluate the effects of oral and injectable omeprazole on basic urinary parameters and selected interleukin levels, with particular emphasis on the potential risk ...
The effects of clomiphene citrate on reproductive parameters in the mare.
Journal of equine veterinary science    August 11, 2026   106125 doi: 10.1016/j.jevs.2026.106125
Maxwell HE, Lyman CC, Foradori CD, Moore HL, Chaves-Cordoba B, Armstrong CL, Klabnik JL.Clomiphene citrate (CC) is a longstanding human fertility drug that has yet to be investigated in horses. The objective was to evaluate effects of CC on mare reproduction. After follicle ablation on Day 0, treatment mares (n=5) received 2 grams of CC intravenously on Days 1-5 while control mares (n=4) received no treatment. Transrectal ultrasonography was performed on Days 1-5 and 8, and transvaginal oocyte aspiration occurred on Day 8. Hormones were analyzed on Days 0-5, 8, 11, and 15. Mares receiving CC exhibited higher uterine edema grades compared to control mares (P=0.009). Antral follicl...
Concurrent Small Intestinal Neoplasia And Large Colon Volvulus In A Standardbred Yearling.
Journal of equine veterinary science    August 10, 2026   106124 doi: 10.1016/j.jevs.2026.106124
Barros V, Cascais J, Holbert K, Ruby R, Freeland R.Large colon volvulus is a common, life-threatening cause of colic in horses. Gastrointestinal neoplasia is a rare cause of abdominal pain in young horses. A 1-year-old Standardbred colt presented with acute colic unresponsive to medical treatment. An exploratory laparotomy identified extensive gastrointestinal pathology, and euthanasia was elected due to a poor prognosis. Necropsy revealed a 360-degree volvulus of the caecum and large colon with adhesions, enterotyphlocolitis, and a well-circumscribed mesenteric mass arising from the small intestine. Histopathology in combination with immunohi...
Inter- and intra-rater reliability of linear scoring in warmblood breeding.
Journal of equine veterinary science    August 10, 2026   106107 doi: 10.1016/j.jevs.2026.106107
Weigt A, Brockmann A, Tetens J, Schmitt AO.Reliable phenotypes are essential for successful animal breeding programmes. Subjective phenotypic assessment may lead to under- or overestimation of heritability estimates and thereby affect the power of genome-based analyses. In Warmblood horses, conformation traits are commonly assessed using linear scoring, but its reliability has not been sufficiently quantified. Objective: In this study, we investigated inter- and intra-rater reliability of linear scoring of conformation traits in German warmblood horses. Methods: Professional equine breeding experts (e.g. breeding judges) completed an o...
Leptomeningeal dissemination of a poorly differentiated carcinoma involving the trigeminal ganglion in a horse: a case report.
Journal of equine veterinary science    August 8, 2026   106123 doi: 10.1016/j.jevs.2026.106123
Rocha CSV, Ferreira CS, Jesus CVA, Silveira MDL, Gomes KMC, Júnior VAS, Gobbi FP, Fonseca SR, Oliveira AAF.Neoplastic conditions involving cranial nerves are uncommon in horses and represent a significant diagnostic challenge. Leptomeningeal dissemination is a recognized mechanism of tumor progression in aggressive neoplasms but is rarely reported in this species. A 15-year-old mixed-breed horse with a history of periocular squamous cell carcinoma treated by enucleation three years earlier developed progressive neurological signs. Initial differential diagnoses included metastatic neoplasia and equine protozoal myeloencephalitis. Due to rapid neurological deterioration, euthanasia and necropsy were...
The effect of intravenous fluids administered prior to competition: A randomized clinical.
Journal of equine veterinary science    August 8, 2026   106121 doi: 10.1016/j.jevs.2026.106121
Weintraub L, Fielding CL, Carli I, Greene-Dittz G.Administration of intravenous fluids prior to competition is common at major equestrian competitions, yet few studies have evaluated the benefits of this practice. Objective: The hypothesis was that pre-ride intravenous fluid therapy would be associated with a lower heart rate and improved laboratory hydration parameters during or after the ride. Methods: 14 client owned horses entered in a 45 km ride in extreme heat and terrain were randomly assigned to receive IV fluids (IVF) or no IV fluids (NIV) the day before the ride. Blood samples and physical examination findings were collected at 6 ti...
Effects of quercetin supplementation in cooling extender on sperm quality of stallions.
Journal of equine veterinary science    August 7, 2026   Volume 166 106122 doi: 10.1016/j.jevs.2026.106122
Silva LFMC, Papa FO, Petelinkar IM, Freitas-Dell'Aqua CP, de Oliveira SN, de Araujo EAB, Junior LRPA, Rodrigues LT, Monteiro GA.Oxidative stress from excessive reactive oxygen species (ROS) during cooling impairs equine sperm quality. Quercetin, a flavonoid with antioxidant properties, has been proposed to reduce oxidative damage in cooled semen. Objective: This study aimed to identify a suitable quercetin concentration among those tested for cooled equine semen and evaluate its effects on sperm kinematics, membrane integrity, and oxidative stress parameters. Methods: Four experiments were conducted. Experiment 1 (n = 8 ejaculates, 4 stallions) evaluated quercetin at 0, 20, 30, or 45 µg/mL at 5°C for 24 h. Experiment...
Short-term effects of pulsed electromagnetic field therapy on subjective flexion-associated lameness responses and circulating cytokines in horses with distal tarsal osteoarthritis.
Journal of equine veterinary science    August 5, 2026   106106 doi: 10.1016/j.jevs.2026.106106
Wilson I, Kiger K, Reynolds S, Justice M, Kapadia C, Coleman SJ, Crook RA, Fedorka CE.Pulsed electromagnetic field (PEMF) therapy is commonly used in equine sports medicine for its proposed anti-inflammatory effects; however, limited controlled studies have evaluated its physiologic effects in horses. Objective: The objective of this study was to investigate the effects of PEMF therapy on flexion-induced lameness and circulating cytokines in horses with radiographic evidence of distal tarsal osteoarthritis. Methods: Six mares were enrolled in a repeated-measures crossover design in which each horse served as its own control. Horses were evaluated at baseline (sham PEMF) and at ...
Evaluation of Serum Specific IgE Sensitization Profiles in a Korean Domestic Horse Population Using a Multiplex Allergen Line-blot Assay: A Cross-Sectional Screening Study.
Journal of equine veterinary science    August 5, 2026   106118 doi: 10.1016/j.jevs.2026.106118
Cho GJ, Jeon J, Eun JK, Almazbek I.The purpose of this study was to evaluate the clinical applicability of a multiplex serum specific Immunoglobulin E (sIgE) test kit 'ANITIA Equine IgE' for identifying the causes of allergic diseases in horses raised in Korea, and to determine the major sensitization antigen profiles in the domestic horse population. Serum or plasma samples were collected from a total of 140 horses, classified by management system (Group A, n=108) and purpose (Group B, n=32). IgE concentrations for 120 environmental and dietary allergens were measured using a multiplex line-blot assay based on an indirect immu...
Comparison of xylazine-ketamine-guaifenesin and xylazine-tiletamine-zolazepam protocols for constant rate infusion anesthesia in horses undergoing field castration.
Journal of equine veterinary science    August 3, 2026   106109 doi: 10.1016/j.jevs.2026.106109
Maneephan P, Thepparat K, Suwanekkasit B, Limrodpai J, Sangbunjong A, Karkkaew P, Chantong B, Vinijkumthorn R.The common use of "triple drip" (xylazine, ketamine, and guaifenesin; XKG) is a popular protocol for constant rate infusion (CRI) in equine practice. However, due to restrictions on ketamine and guaifenesin in many countries, tiletamine-zolazepam (TZ) could serve as an alternative. To date, there have been limited studies evaluating the use of xylazine in combination with TZ (XTZ) administered via CRI to maintain surgical anesthesia in equine field practice. Objective: To compare the effectiveness and safety of XTZ infusion with the classical XKG protocol for field anesthesia. Methods: Twelve ...
Seroprevalence, risk factors and hematological parameters associated with Equine Infectious Anemia from Casanare, Colombia.
Journal of equine veterinary science    August 3, 2026   106108 doi: 10.1016/j.jevs.2026.106108
Puentes MMM, Guzman YL, Torreglosa JCT, Isaza PAL, Guzman-Barragán BL.Equine infectious anemia (EIA) is a persistent lentiviral disease with important sanitary and economic implications in tropical regions; however, epidemiological information in Colombia remains limited. Objective: To estimate seroprevalence, identify risk factors, and evaluate hematological alterations associated with EIA in horses from Casanare, Colombia. Methods: A cross-sectional study including 1,228 horses from 204 production units across 121 rural districts. Serological diagnosis was performed using agar gel immunodiffusion. Demographic and management data were obtained through an epidem...
Ultrasonographic assessment of in utero fetal presentation, position and posture can be essential: case report.
Journal of equine veterinary science    August 3, 2026   Volume 166 106105 doi: 10.1016/j.jevs.2026.106105
Renaudin C, Omyla K, De Castro T, Ratliff B, Bartholomew S, Dechant J, Vapniarsky N, Woolard K, Kovacs S, Bozorgmanesh R, de Mestre AM, Dini P.The objectives of this clinical case report were to highlight the value of late transrectal ultrasound examination for assessing fetal presentation, position and posture and to describe the management of a case of malposture associated with musculoskeletal and cerebellar abnormalities, including its in utero ultrasound diagnosis and outcome. A 7-year-old Gypsy Vanner mare, 339 days pregnant, was diagnosed with the presence of a bony fetal structure cranial to the cervix, without palpable head or limbs and minimal fetal movement. Transrectal ultrasound examination was instrumental in diagnosing...
Hypothalamic-pituitary-adrenal axis response and glucose homeostasis during exercise to fatigue before and after training in old and young adult Standardbred mares.
Journal of equine veterinary science    July 28, 2026   106103 doi: 10.1016/j.jevs.2026.106103
Liburt NR, Herbst AC, Malinowski K, McKeever KH.While reduced cortisol responses to exercise have been described in older horses, the effects of aging and training on the hypothalamic-pituitary-adrenal axis (HPA axis) and downstream metabolic signaling are currently unknown. Unassigned: This study tested the hypothesis that age and exercise training alter the response of the HPA axis, insulin, and glucose during an acute exercise challenge to fatigue. Methods: Six old (22.0±0.7 years) and six young adult (7.3±0.6 years) unfit Standardbred mares were tested. Mares ran a graded exercise test before (GXT1) and after (GXT2) 8 weeks of trainin...
Oxidative Stress and Immunomarkers in Equine Dermal Melanomatosis: Single-Case Comparison with Healthy Individual.
Journal of equine veterinary science    July 28, 2026   106104 doi: 10.1016/j.jevs.2026.106104
Dobrin AA, Dungu C, Stanciu AG, Gagniuc E, Stan MS, Militaru M.Dermal melanomatosis is a common tumor in grey horses, characterized by cutaneous/subcutaneous lesions with metastatic potential. While clinically well-described, oxidative stress involvement remains poorly understood. This case report compared oxidative stress markers and antioxidant enzymes' activities in total protein extracts from melanomatosis lesions (grey horse) versus healthy control skin (black horse), alongside histopathology and immunohistochemistry, to correlate oxidative imbalance and tumor behavior. Melanomatosis tissue exhibited decreased antioxidant defenses confirmed by catala...
Combined effects of pre-freeze cholesterol-loaded cyclodextrin and post-thaw pentoxifylline on the functional longevity of cryopreserved stallion sperm.
Journal of equine veterinary science    July 28, 2026   Volume 165 106100 doi: 10.1016/j.jevs.2026.106100
Vilela VMT, Zutter BM, Miranda BN, Baldini PHO, Gomes LLO, Silva DF, Diniz JHW, Sancler-Silva YFR, Ignácio FS, Papa FO, Monteiro GA.Cryopreservation causes structural and metabolic damage to stallion spermatozoa, impairing post-thaw functional competence. Objective: This study evaluated whether cholesterol-loaded cyclodextrin (CLC) supplementation before freezing, combined with pentoxifylline (PTX) after thawing, improves the functional quality, oxidative status, mitochondrial activity, and preliminary fertility outcomes of cryopreserved stallion semen. Methods: Semen from twelve stallions was assigned to four CLC concentrations (0, 1, 1.5, and 2 mg), frozen, thawed, and divided into subgroups with or without PTX supplemen...
Effects of a dimethylformamide-glycerol combination on freezing behavior, post-thaw sperm quality, and sperm-oocyte interaction in Arabian stallions.
Journal of equine veterinary science    July 28, 2026   Volume 166 106102 doi: 10.1016/j.jevs.2026.106102
Soria M, Ochoa-Guillén J, Álvarez-Palacios D, Sacaquirín-Rivadeneira A, Pillacela J, Duma M, Bermúdez G, Galarza L, Galarza DA.Although glycerol (GLY) and dimethylformamide (DMF) are widely used cryoprotectant agents (CPAs) for freezing equine semen, their combined use remains less characterized. Objective: To evaluate the effects of DMF, GLY, and their combination (DMF+GLY) on freezing behavior, sperm kinematics, membrane integrity, and IVF-related functional endpoints in Arabian stallion sperm. Methods: Twenty-four ejaculates from four stallions (six/male) were diluted in BotuSemen-Gold® extender supplemented with 5% DMF, 5% GLY, or 3% DMF+ 3% GLY, packed into 0.5-ml straws, and frozen. Sperm-oocyte binding and pro...