Analyze Diet

Journal of equine veterinary science.

Periodical
Veterinary Medicine
Horses
Publisher:
William E. Jones,. New York : Elsevier (2003)
Frequency: Monthly
Country: United States
Language: English
Start Year:1981 -
ISSN:
0737-0806 (Print)
0737-0806 (Linking)
Impact Factor
1.3
2022
NLM ID:8216840
(DNLM):J20290000(s)
(OCoLC):07643410
Classification:W1 JO644CD
Bilateral hindlimb superficial digital flexor tendinopathy in a sports horse.
Journal of equine veterinary science    February 20, 2026   Volume 159 105819 doi: 10.1016/j.jevs.2026.105819
Mazzucco L, Marcatili M, Withers J, Cantatore F.Superficial digital flexor tendinopathy is a common cause of forelimb lameness in Thoroughbred racehorses and sports horses; however, this condition is rarely observed in the hindlimb. A 16-year-old Irish sports horse gelding was presented with bilateral swelling in the plantar metatarsal region and severe bilateral hindlimb lameness visible at the walk. The lameness occurred after the cross-country phase of an eventing competition. The clinical suspicion of superficial digital flexor tendon (SDFT) injury was confirmed after ultrasonographic examination, which revealed an extensive core lesion...
Effect of feeder style on intake rate of equine concentrates.
Journal of equine veterinary science    February 19, 2026   Volume 159 105811 doi: 10.1016/j.jevs.2026.105811
O'Reilly K, Aldworth-Yang S, Catalano DN.There are many different concentrate feeders available to horse owners, but little data exists on feeder effect of equine intake rate. Rapid consumption ("bolting") is associated with issues such as esophageal obstruction. Objective: The objective of this study was to evaluate three common concentrate feeders on intake rate. We hypothesized horses would have the slowest intake rate when fed from the trough. Methods: Four geldings were fed 0.454 kg of concentrate from a pan on the ground (P), shallow trough (T), or hanging feeder (H). Horses were randomly assigned a first feeder and then rotate...
Proctectomy with coloanal anastomosis in horses – a cadaveric study.
Journal of equine veterinary science    February 18, 2026   Volume 159 105814 doi: 10.1016/j.jevs.2026.105814
Strugava L, Serighelli-Júnior G, Stadler LM, Flores LADS, Minozzo GA, Dornbusch LPTC, Moreno JCD, Dornbusch PT.Rectal amputation is a surgical approach indicated for the management of severe rectal lacerations or extensive rectal prolapse when transmural compromise or primary repair is unfeasible. These conditions, although uncommon, present substantial challenges in equine practice due to the anatomical complexity of the pelvic region and the potential for life-threatening complications if not adequately addressed. This study aimed to describe and standardize a reproducible surgical technique for rectal amputation in horse cadavers. Six equine cadavers (mean body mass, 441.83± 66.84 kg) were position...
Confocal laser endomicroscopy: exploring endometrial image quality in relation to the oestrous cycle of mares.
Journal of equine veterinary science    February 18, 2026   Volume 160 105815 doi: 10.1016/j.jevs.2026.105815
Gallacher K, Woolford L, Gilbert RO, Santos LC, McLeod J, Hebart M, Agne GF, du Preez S, Kind KL.Assessment of the endometrium is an essential component of a breeding soundness examination in mares. The current reference standard for evaluating equine endometrial health is histopathological assessment of an endometrial biopsy, however, inconsistencies may arise when a single blinded biopsy is used. Confocal laser endomicroscopy (CLE) is non-invasive and enables real-time in-vivo imaging of the endometrium across multiple locations within the uterine lumen. An initial study revealed variation in CLE image quality between mares. Objective: This study aimed to compare the quality of endometr...
Effect of palmar digital perineural analgesia (as a model for “nerving”) on forelimb loading over varied surfaces at the walk and trot – a pilot study.
Journal of equine veterinary science    February 17, 2026   Volume 159 105812 doi: 10.1016/j.jevs.2026.105812
Zimmerman-Cameron SR, Colbath AC, Caruso MA, Hoffman RM, Logan AA.Navicular syndrome is characterized by palmar foot pain and is a common cause of lameness in horses. Palmar digital neurectomy (nerving) removes sensation to the heel region of the hoof and may serve as a treatment for navicular-afflicted horses, while analgesia (blocking) results in temporary loss of sensation. Objective: The purpose of this pilot study was to examine the effect of loss of sensation in the heel on the forelimb area and force while tracking on soft and hard ground at the walk and trot. Methods: Three horses that scored a 0 or 1 on the AAEP lameness scale were fitted to Tekscan...
Hemithyroidectomy due to a large, solid follicular thyroid adenoma in a 19-year-old thoroughbred mare.
Journal of equine veterinary science    February 16, 2026   Volume 159 105810 doi: 10.1016/j.jevs.2026.105810
Bramall LM, Hinnigan GJ, Wattam AD, Owen KR.This case report describes the successful hemithyroidectomy of the largest equine solid follicular adenoma described in the veterinary literature to date. A 19-year-old thoroughbred mare was referred to Oakhill Veterinary Centre for the surgical removal of a large, left-sided thyroid gland measuring 26cm in diameter. Pre-operative considerations, the hemithyroidectomy surgical technique as well as the intra- and post-operative complications encountered, are discussed. Following surgery the horse had no long-term complications and continues to thrive four and a half years post-operatively.
Verifying thoroughbred racehorse fatalities through Equibase chart review.
Journal of equine veterinary science    February 16, 2026   Volume 159 105808 doi: 10.1016/j.jevs.2026.105808
Markowitz JS.Equibase (EQB) race charts provide the official record of North American Thoroughbred races and often document catastrophic injury, euthanasia, or equine-ambulance transport. Because chart documentation and regulatory classification differ, fatalities occurring > 72 hours after a race fall outside international regulatory definitions applied by the Equine Injury Database (EID) and may not appear in EID aggregates. EQB charts confirm horse identity, race participation, finishing status, and last recorded start. Horseracing Wrongs (HRW), an advocacy group, publishes horse-level fatality data ...
Radiographic findings of candidate stallions presented for licensing at all German Warmblood horse-breeding associations in 2018-2020.
Journal of equine veterinary science    February 16, 2026   Volume 159 105809 doi: 10.1016/j.jevs.2026.105809
Folgmann MS, Stock KF, Feige K, Delling U.Current studies on the health status of young German Warmblood stallions are lacking. Objective: To evaluate the prevalence of radiographic findings at licensing examinations of Warmblood candidate stallions and quantify the environmental influences on the distribution of recorded findings. Methods: In this retrospective observational study, records of 1693 radiographic examinations performed on 1678 German Warmblood stallions presented for licensing in 2018-2020 were reviewed. Data were provided by all German Warmblood horse-breeding associations and their official veterinarians. The collecti...
In vitro anthelmintic activity of extracts and bioactive compounds from Azadirachta indica and Cnidoscolus angustidens on strongyles L3 larvae in horses.
Journal of equine veterinary science    February 16, 2026   Volume 159 105807 doi: 10.1016/j.jevs.2026.105807
Díaz-Alvarado T, Alcala-Canto Y, Elghandour MMMY, Salem MZM, Mariezcurrena-Berasain MD, Salem AZM.Gastrointestinal endoparasitic diseases, particularly those caused by some strongyles, represent one of the main health challenges in equine production. The prolonged use of synthetic anthelmintics has led to the development of resistance, which has prompted the search for more sustainable therapeutic alternatives. However, plant extracts rich in secondary metabolites are emerging as a promising option. Objective: The objective of this study was to evaluate the in vitro anthelmintic activity of aqueous extracts and bioactive compounds (total phenols and saponins) from Azadirachta indica and Cn...
Antiparasitic potential of Caesalpinia coriaria: Current evidence and prospects for equine health.
Journal of equine veterinary science    February 13, 2026   Volume 159 105806 doi: 10.1016/j.jevs.2026.105806
Castillo JDCR, Forte L, Elghandour MMMY, Maggiolino A, De Palo P, Ponce-Covarrubias JL, Salem AZM.Parasitic infections remain a major constraint to equine health and performance, and the worldwide rise of anthelmintic resistance is forcing a shift toward integrated parasite management and new complementary tools. Caesalpinia coriaria (Divi-divi) pods are exceptionally rich in tannins and have been proposed as a potential source of antiparasitic phytochemicals. This review summarizes the current evidence on C. coriaria, focusing on its relevance to equine gastrointestinal helminths. Most available data come from in vitro screening against ruminant nematode models (mainly Haemonchus contortu...
Blood and fecal metabolic responses of mature horses fed rhizoma peanut hay.
Journal of equine veterinary science    February 11, 2026   Volume 159 105805 doi: 10.1016/j.jevs.2026.105805
Vasco ACCM, Warren LK, Silva GM, Cuervo-Vivas W, Brandi RA, Silva VP, Lance JM, Dubeux JC, Wickens CL.Rhizoma peanut (RP, Arachis glabrata Benth) is an alternative legume forage to alfalfa (ALF, Medicago sativa L.) with less non-structural carbohydrates and crude protein. Yet, metabolic responses to consuming RP hay have never been evaluated in horses. Objective: The objective of this study was to investigate the effect of feeding RP 'Florigraze' hay on fecal and blood metabolic variables compared to alfalfa 'Legendary XHD' and bermudagrass (BMG, Cynodon dactylon L. 'Coastal') hays fed to maintenance horses. Methods: Six mature Quarter Horse geldings (596±40 kg) were randomly assigned to one ...
Spirulina supplementation regulates inflammation and supports cartilage health in adult sedentary horses following moderate-intensity exercise.
Journal of equine veterinary science    February 7, 2026   Volume 158 105804 doi: 10.1016/j.jevs.2026.105804
Golestani NG, Paton MP, Ross BR, Wildish AW, Duarte MSD, Williams CAW, Pearson WP.Horses experience rapid physiological, inflammatory, and oxidative responses during exercise. Spirulina, a nutrient-dense microalga with antioxidant and anti-inflammatory properties, may help modulate these responses and support recovery. Objective: This study evaluated the effects of 30-day dietary Spirulina supplementation on physiological, inflammatory, and oxidative stress responses to moderate-intensity exercise in sedentary horses. We hypothesized that 30-day Spirulina supplementation would modulate hematologic and inflammatory responses and support recovery from moderate-intensity exerc...
Estrus synchronization and follicular development patterns in jennies.
Journal of equine veterinary science    February 5, 2026   Volume 159 105799 doi: 10.1016/j.jevs.2026.105799
Ayiken N, Tuohudashen Y, Huojiaabudula Y, Abudureyimu A, Wang H, Geng M, Hu G, Wang Y, Zeng W.Seasonal variations and breed differences significantly influence reproductive efficiency in jennies, yet optimized, evidence-based estrus synchronization protocols for key Chinese breeds are lacking. Objective: This study aimed to compare the efficacy of three hormonal protocols on estrus synchronization, and to characterize follicular development patterns in DeZhou and Xinjiang jennies across different seasons. Methods: In two experiments (April-May, n = 94; July-August, n = 144), 238 non-pregnant jennies received one of three treatments: GnRH-PGFα with two injection timings (Treatment Grou...
Riding with care: A review of factors that influence the welfare of the ridden horse and a case for the application of the precautionary principle in equestrian pursuits.
Journal of equine veterinary science    February 4, 2026   Volume 158 105801 doi: 10.1016/j.jevs.2026.105801
Copelin C, Merkies K.Equestrian sport's social license to operate has come under scrutiny due to concerns surrounding the well-being of ridden horses. Inappropriate equipment use, such as harsh bits or overtight nosebands, can negatively influence well-being by generating inescapable pressure or pain on the sensitive structures of the horse's head and limiting natural behaviours. Restrictive equipment may also be used to generate exaggerated, stressful and uncomfortable head and neck positions such as hyperflexion. Saddles must be properly fitted to both horse and rider to ensure appropriate distribution of kinema...
Maggot therapy in horses with traumatic wounds caused by wire fences: Case reports.
Journal of equine veterinary science    February 4, 2026   Volume 158 105802 doi: 10.1016/j.jevs.2026.105802
Dos Santos TS, Dillmann JB, Giovelli M, Elias GO, Lima RK, Cescon S, Stainki DR, Dos Santos RC, Corrêa C, Monteiro SG.This study reports the successful use of maggot therapy in four horses with traumatic wounds caused by wire fences, treated in veterinary clinics and under field conditions in southern Brazil. All lesions showed tissue necrosis, purulent discharge, and foul odor, indicating infection and delayed healing. Sterile Lucilia cuprina larvae (L1 stage) were applied directly to the wounds for 48 hours. Rapid improvement was observed, with marked reduction of necrotic tissue, exudate, and odor, and stimulation of granulation tissue in three cases. Complete wound closure occurred between 10 and 60 days ...
Congenital malformations of the thoracic vertebral column in a mature competition pony.
Journal of equine veterinary science    February 4, 2026   Volume 158 105800 doi: 10.1016/j.jevs.2026.105800
Hoogelander B, Frippiat T, Bergman HJ, Vanderperren K, van Veggel ECS.Thoracolumbar congenital abnormalities are considered rare in mature, performing horses. This case report describes a 14-year-old Welsh pony presented with marked epaxial muscle atrophy, mid-thoracic spinal pain, and stiffness, most pronounced during canter. The thoracic spine exhibited scoliosis, centered at T9-T10, and kyphosis. Radiography and postmortem computed tomography revealed complex congenital malformations of the thoracic vertebral column, including abnormal morphology of the T10-T12 vertebral bodies, partial vertebral fusion, a malformed spinous process, a malformed rib with assoc...
Analysis of the antitrypsin activity and immunoglobulin G concentration in equine colostrum and milk.
Journal of equine veterinary science    February 4, 2026   Volume 158 105803 doi: 10.1016/j.jevs.2026.105803
Böckmann S, Trzebiatowski L, Georgiev P, Büttner K, Wehrend A.Immunoglobulins ingested via colostrum must remain intact to be absorbed by the neonate. Equine colostrum contains antitrypsin activity, which likely protects these proteins from proteolysis in the gastrointestinal tract. Objective: To quantify antitrypsin activity in equine colostrum and milk, describe its temporal changes during the first five days after parturition, compare actitvity between left and right mammary glands, evaluate differences between nulliparous and pluriparous mares, and examine associations with immunoglobulin G (IgG) concentrations and mare age. Methods: This retrospecti...
Translational human and equine regenerative medicine in musculoskeletal conditions.
Journal of equine veterinary science    February 1, 2026   Volume 158 105798 doi: 10.1016/j.jevs.2026.105798
Bosman LM, Logan MG, Miszewski A, Pepper MS.Translational regenerative medicine, integrating human and veterinary approaches within the "One Health" framework, increasingly uses horses as models for human musculoskeletal conditions due to shared anatomical and functional features. Osteoarthritis and tendon disorders affect both species, often resulting from high-impact or repetitive strain activities. Regenerative medicine offers therapeutic opportunities by promoting tissue repair and modulating inflammation. Cellular orthobiologics such as mesenchymal stromal/stem cells (MSCs) show promise for treating osteoarthritis and tendon injuri...
miRNAS and target genes are regulated post-training in quarter horse athletes.
Journal of equine veterinary science    January 20, 2026   Volume 158 105787 doi: 10.1016/j.jevs.2026.105787
Amaral TM, Ramos PDCA, Silva RA, Ferreira MM, Filho EDS.In the context of Brazilian equestrian sports, the Quarter Horse (QH) breed has the third largest population of registered horses and stands out for its numerous abilities in various disciplines. Studies on equine health and athletic performance parameters are scarce, especially involving molecular markers. Objective: This study aimed to investigate the differential expression of microRNAs and target genes involved in metabolic pathways of exercise and to assess hematological and clinical changes in 10 horses (Equus caballus Linnaeus) of the QH breed under different physiological conditions. H...
Evaluation of oxidative stress in the newborn foal: a multibiomarker approach.
Journal of equine veterinary science    January 20, 2026   Volume 158 105786 doi: 10.1016/j.jevs.2026.105786
Bindi F, Cingottini D, Sorvillo B, Sala G, De Marchi L, Monni G, Celardo S, Bonelli F, Sgorbini M.During the perinatal period, neonates experience a sudden increase in oxygen exposure, predisposing them to oxidative stress. Objective: This prospective, in vivo study aimed to evaluate biomarkers of oxidative stress and antioxidant defence in healthy neonatal foals during the first 48 hours of life. Methods: Ten Standardbred foals were enrolled. Blood samples were collected at birth, 24 hours, and 48 hours after birth and analyzed for biomarkers using spectrophotometry assays. Statistical analysis was performed using non-parametric tests and linear regression. Results: The results revealed s...
Left and right-side echocardiographic measurement of pulmonary artery stiffness in thoroughbred racehorses.
Journal of equine veterinary science    January 19, 2026   Volume 158 105784 doi: 10.1016/j.jevs.2026.105784
Bozzola C, Stucchi L, Sala G, Schinardi L, Stancari G, Zucca E.Background: Pulmonary Artery Stiffness (PAS) is a non-invasive echocardiographic index of pulmonary artery elasticity. In horses, PAS has been measured only from the right parasternal short-axis view, while the influence of alternative imaging windows remains unknown.Aims/objectivesTo assess the repeatability and reproducibility of Acceleration Time (AT), Maximal Frequency Shift (MFS), and PAS measurements from the left parasternal angled view of the right ventricular inflow/outflow, and to compare these parameters with those obtained from the right parasternal short-axis view in Thoroughbred ...
Infertility in an American Quarter Horse mare with cervical duplication.
Journal of equine veterinary science    January 19, 2026   Volume 158 105785 doi: 10.1016/j.jevs.2026.105785
Cortes D, Mendoza M, Raudsepp T, Ferrer M.Cervical duplication is a disorder of sexual development (DSD) that may be present in the absence of chromosomal aberrations. It is a rare cause of infertility in mares and is attributed to the failure of the Müllerian ducts to fuse properly, leading to the persistence of the median walls along the whole length of the cervix. This case report involves a 13-year-old American Quarter Horse mare with cervical duplication. Prior to this diagnostic, the mare was bred multiple times, but pregnancy was not achieved. The mare presented for a breeding soundness evaluation. During the examination an ab...
Effects of sample storage time and temperature on equine complete blood counts.
Journal of equine veterinary science    January 17, 2026   Volume 158 105782 doi: 10.1016/j.jevs.2026.105782
Friend MM, McGaffigan EM, Hall SM, Staniar WB, Smarsh DN.Equine blood samples are often collected in uncontrolled, distant environments and stored for later analysis. Objective: This study aimed to determine how sample storage methods influence complete blood count (CBC) parameters, hypothesizing that temperature and time elapsed from collection to analysis would affect CBC parameters. Methods: Blood samples were collected from 10 healthy Quarter Horse mares (age: 13 ± 4.5 years old) and stored in EDTA tubes at room temperature (22°C), above ice (8°C), refrigerated (4°C), or in contact with ice (2°C). The IDEXX ProcyteDx was used to obtain CBCs...
Two cases of primary hepatic neoplasia in young horses: Diagnostic challenges and clinical implications.
Journal of equine veterinary science    January 16, 2026   Volume 158 105783 doi: 10.1016/j.jevs.2026.105783
Siwinska N, Ciaputa R, Dlugopolska D, Paszkowska M, Haider W, Schwegler K.Primary hepatic neoplasms are rare in horses and often have a poor prognosis due to non-specific clinical signs and delayed diagnosis. This report describes two cases of primary hepatic neoplasia in young horses. The first case was a yearling Hanoverian filly presenting with non-specific clinical signs, including poor weight gain and inappetence, ultimately diagnosed with hepatoblastoma based on ultrasonographic findings and histopathological examination. The second case was a three-year-old Polish Sport Horse stallion that died suddenly, with post-mortem examination revealing advanced cholang...
Parasympathetic tone, cardiovascular variables, and behavioral alterations in conscious horses before and after castration and during anesthesia: A pilot study.
Journal of equine veterinary science    January 9, 2026   Volume 158 105781 doi: 10.1016/j.jevs.2026.105781
Mendoza Flores JE, Terrazas A, Lara Sagahon AV, Aleman M.Parasympathetic tone activity (PTA) and its association with behavior in conscious versus anesthesia in horses are unknown. Objective: To study PTA, heart rate (HR), minimum alveolar concentration (MAC), and mean arterial pressure (MAP) during castration under anesthesia. To study PTA, HR, Horse Grimace Scale (HGS), locomotor activity (LA), and behavior in conscious horses before and after castration. Methods: Pilot study including seven healthy adult horses. After habituation (days 1, 2, and 3), PTAm, HR, HGS, LA, and behavior were investigated during pre-surgery (days 4-, 5-, and one-hour pr...
Bacterial contamination of covered versus non-covered plates in a sterile operating room in an equine referral center.
Journal of equine veterinary science    January 7, 2026   Volume 157 105775 doi: 10.1016/j.jevs.2026.105775
Dersch K, Kreuder A, Wang C, Troy J.Surgical site infections (SSI) are a common postoperative complication of equine orthopedic surgery and often increase morbidity and mortality. This study aimed to determine if covering surgical instruments during surgery decreased bacterial environmental air contamination during early opening of surgical packs (EOSP). The authors hypothesized that covering instruments with sterile surgical towels would reduce the total number of bacterial colonies to which the instruments were exposed. Blood agar plates (BAP) were allocated into two groups (non-covered vs. covered with a sterile huck towel)...
Impact of melatonin on inflammatory cytokine profiles in 24-hour cultured equine uterine explants’.
Journal of equine veterinary science    January 7, 2026   Volume 157 105776 doi: 10.1016/j.jevs.2026.105776
Rodrigues MF, Neves AP, Ferreira R, Pessoa GA, Araujo DN, de Souza GV.Melatonin has demonstrated beneficial effects in regulating inflammatory processes in various tissues, but its role in mare uterine physiology remains unclear. Objective: To investigate the effects of melatonin on the gene expression of cytokines (IL-1β, IL-6, IL-8, IL-10, and TNF-α) in equine uterine explants cultured for 6, 12, or 24 hours. Methods: The study was divided into two experiments. Part 1 aimed to evaluate the cytotoxicity of melatonin at concentrations of 0, 0.5, 1, 2, 4, and 8 mM on endometrial cells. Part 2 aimed to determine the expression of inflammatory cytokines in uterin...
Antibiotics prescribing patterns and association with system-specific pathologies in equine veterinary practice: Insights from a 12-year study at a veterinary teaching hospital.
Journal of equine veterinary science    January 7, 2026   Volume 157 105779 doi: 10.1016/j.jevs.2026.105779
Vernaccini M, de Marchi L, Bindi F, Meucci V, Sala G, Cingottini D, Sgorbini M.Antibiotics are essential in veterinary medicine, enhancing animal health and welfare. However, widespread use contributes to resistance, increasing the risk of treatment failure and the transmission to humans. Objective: Analyze antibiotic prescribing practices in an equine internal medicine unit, focusing on factors influencing use, particularly Highest Priority Critically Important Antimicrobials (HPCIAs). Methods: 1315 adult equids, including horses, donkeys and mules, presented for different diseases to the Veterinary Teaching Hospital (VTH) of the University of Pisa from 2011 to 2023. Re...
Effects of intrauterine ozone insufflation in eleven subfertile mares: a case series.
Journal of equine veterinary science    January 7, 2026   Volume 157 105778 doi: 10.1016/j.jevs.2026.105778
Moroni R, Fanelli D, Maltinti S, Orefice M, Rota A, Camillo F, Melanie P, Cantile C, Miragliotta V, Pirone A, Lazzarini G, Passamonti F, Marmorini P....Equine persistent breeding-induced endometritis can lead to chronic uterine inflammation and fibrosis, reducing fertility. Intrauterine ozone (O₃) has recently been proposed as a potential treatment for mares unresponsive to conventional therapies. This case series describes O₃ treatment in eleven mares barren for at least one year despite multiple inseminations attempts. During the first oestrous cycle, low-volume uterine lavage and endometrial biopsy were performed, followed by 3 consecutive days of intrauterine O₂-O₃ gas insufflation. In the subsequent cycle, both procedures were re...
Metabolomics of stallion seminal plasma: Identification of potential fertility biomarkers based on differences between high and low pregnancy stallions.
Journal of equine veterinary science    January 6, 2026   Volume 158 105780 doi: 10.1016/j.jevs.2026.105780
Bueno VLC, Bastos HBA, Centeno LAM, Schmitt F, Larentis G, Mattos RC, Fiala-Rechsteiner S.The seminal plasma (SP), a fluid originating from the testes, epididymis, and accessory sex glands, represents 98 % of the volume of stallion ejaculate. Metabolomic analysis enables the identification of final products of metabolic pathways, providing insights into reproductive physiology. Objective: This study aimed to investigate the metabolomic profile of stallion seminal plasma and to investigate metabolic differences between stallions with high and low pregnancy rates, in order to identify potential biomarkers associated with fertility. Methods: Twenty-four Criollo stallions, were sampled...
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