Topic:Animal Health
Animal Health encompasses a broad range of topics focused on maintaining and improving the well-being of equine species. This field addresses various aspects of horse care, including disease prevention, diagnosis, treatment, and management of health conditions. Key areas of interest include equine nutrition, vaccination protocols, parasite control, dental care, and the management of chronic conditions such as laminitis and colic. Additionally, animal health research in horses investigates the impact of exercise and training on physical health, the role of genetics in disease susceptibility, and the development of new therapeutic approaches. This page compiles peer-reviewed research studies and scholarly articles that explore the latest advancements, challenges, and best practices in preserving and enhancing the health of horses.
Ultrasound-Guided Radiofrequency Ablation and Pulsed Radiofrequency Treatment for Chronic Lameness Due to Distal Forelimb Disease in Horses: A Pilot Study. Radiofrequency ablation (RFA) and pulsed radiofrequency (PRF) are non-pharmacological techniques employed in humans for chronic pain, but their veterinary application is unexplored. This pilot study evaluated clinical effects of RFA and PRF in twenty-four horses with chronic distal forelimb lameness. Ultrasound-guided RFA (N = 8; 60-90 °C, 2-8 min) or PRF (N = 16; 42 °C; 12 min) was applied to palmar digital nerves. Lameness was scored (American Association of Equine Practitioners scale) at baseline and monthly for six months (T1-T6). At T2, partial- and non-responders in both groups receive...
Radiographic area of large intestinal sand accumulation in horses may determine clinical significance. Sand enteropathy is a common disease in horses worldwide. The presence of sand does not always cause disease. The amount of sand required to cause clinical disease is not well established. Objective: To establish a weight-indexed cut-off for the diagnosis of clinically relevant sand enteropathy based on radiographic area. Methods: Cross-sectional. Methods: Abdominal radiographs were acquired from clinically normal horses and compared with horses with clinical sand enteropathy. Sand area and sand area per kilogram of body weight were calculated. A receiver operating characteristic (ROC) curve w...
Seroprevalence of Toxoplasma gondii in Equids: A Global Systematic Review and Meta-analysis. Toxoplasmosis is a zoonotic infectious disease caused by T. gondii, a widely distributed obligate intracellular parasite. It has received widespread attention because it can infect a large number of domestic and wild animals, thereby posing a threat to public health and the economy on a global scale. Among them, equine animals are critical intermediate hosts. However, studies on T. gondii infection in equine animals are currently not systematic on a global scale. For this purpose, we conducted a systematic review and meta-analysis. Methods: This study searched for relevant studies in six signi...
Probing Wnt pathway and functional signal in equine melanocytic neoplasms through quantitative proteomics and immunohistochemistry. Equine melanocytic neoplasm (EMN) is a skin tumor commonly observed in grey horses. Limited research has yet to investigate proteomic profiles of EMN, particularly in the early stages and their expression patterns. This study, therefore, aimed to identify signature proteins from tissue biopsies to distinguish early EMN, severe EMN, and normal groups. Results: Using proteomic analysis of 19 tissue samples (normal: n = 6, early EMN: n = 7, severe EMN: n = 6) through LC-MS/MS, 12,310 proteins were identified. Differentially expressed proteins (DEPs) and functional interaction analysi...
LMF1 frameshift deletion in Franches-Montagnes horses with hypertriglyceridemia-induced pancreatitis. Hypertriglyceridemia (HTG) may be inherited and caused by variants in genes encoding enzymes of lipid metabolism. This study was prompted by the observation of eight Franches-Montagnes (FM) foals showing elevated plasma triglyceride levels and episodes of fatal acute pancreatitis. We termed this phenotype hypertriglyceridemia-induced pancreatitis (HIP). The affected foals were distantly related and inbred to a prominent stallion suggesting autosomal recessive inheritance. Whole genome sequencing of an affected foal identified a homozygous loss of function variant in LMF1 encoding lipase matura...
Seasonality, control, and risk factors for Gasterophilus intestinalis egg intensity in horses from Romania under field conditions. This study aimed to assess the efficacy of pyrethroid-based insecticides against Gasterophilus spp. egg-laying activity and to establish the seasonality patterns in the specific climatic area of Transylvania, Romania. To fulfil the aims of the study, a total of 40 horses were treated every 4 weeks and inspected every 2 weeks between 11 May and 6 November (Pilo et al. Parasitol Res 114:1693-1702, 2024). Through this period, eggs were found between 24 June and 2 November. The eggs were found on 34 out of 40 horses (prevalence = 85%, CI 95% = 73.43-96.56%). Overall, a total of 50,029 G...
Management of retained fetal membranes by umbilical vessel infusion in mares submitted to elective C-section. The cesarian section (C-section) is a potential risk factor for retained fetal membranes (RFM) in mares, requiring prompt diagnosis and treatment to enhance outcomes. This study aimed to evaluate the use of umbilical vessel infusion for the treatment of RFM after elective C-section in mares. Ten pregnant criollo type-mares at 315 days of gestation were monitored daily and underwent elective C-section upon readiness for birth. All mares exhibited RFM, with placentas retained beyond three hours careful extraction of the foal by C-section. Those were randomly assigned into two groups: Umbilical v...
Plasma Estradiol and Endometrial Edema Profile in Acyclic Mares After Single Administration of 17-β Estradiol, Estradiol Benzoate and Estradiol Cypionate. A longer endometrial exposure to estradiol before progesterone has been shown to be beneficial in cyclic and acyclic recipient mares. Therefore, the selection of an estradiol ester that promotes longer endometrial exposure to estradiol using a single administration would be advantageous when preparing acyclic mares as embryo recipients. This study investigated plasma estradiol profiles in acyclic mares after a single administration of 17-β estradiol (17-β), estradiol benzoate (EB) and estradiol cypionate (EC), and the correlation between plasma concentrations and endometrial edema. Fifteen n...
Isolation of vapA-positive Rhodococcus equi from soil and fecal samples in Mongolia. In 2024, 90 soil samples and 11 fecal samples were collected from nine Mongolian provinces. Using NANAT selective agar, R. equi was successfully isolated from 23 soil samples (25.6%) across five provinces and from three fecal samples (27.3%) collected in two provinces. A total of 122 isolates were identified as R. equi via choE-targeted polymerase chain reaction (PCR) and subsequently screened for virulence-associated genes (vapA, vapB, and vapN) by PCR. Of these, 17 isolates tested positive for the vapA gene, while the remaining 105 isolates were negative for both vapB and vapN. Plasmid prof...
α2-Macroglobulin Concentrations in Canine and Equine Serum Donors. To determine if serum α2-macroglobulin (A2M) concentration varies based on donor species-canine or equine-and signalment. Methods: Serum A2M concentration ([A2M]) was measured in healthy dogs (n = 30) and horses (n = 31) using species-specific ELISAs. Results: Canine and equine [A2M] median (IQR; range) were 98.70 ng/mL (92.79 ng/mL; 34.33-696.18 ng/mL) and 557 000 ng/mL (437 900 ng/mL; 62 600-3 042 900 ng/mL), respectively. Equine [A2M] was significantly higher than canine [A2M]. Depending on the statistical analysis performed, sex had either no statistical (pâ€...
Seroprevalence of equine leptospirosis in Poland (2019-2023). Leptospirosis in horses is associated with various clinical signs, potentially leading to fatal outcomes. Additionally, the disease may pose a zoonotic risk to individuals involved in handling infected animals. Implementing a serological monitoring programme in the equine population is one of the key tools used to reduce the risk of transmission of Leptospira infections to humans. Objective: To provide new insights into the seroprevalence of leptospirosis in domestic horses in Poland. Methods: Serological monitoring program. Methods: Data were collected from serological surveys of horse serum ...
Surgical Assessment and Post-Operative Complications Following Video-Assisted Thoracoscopic Surgery (VATS) of Horses with Severe Equine Pasture Asthma During Asthma Exacerbation and Remission. The aim of this retrospective clinical study was to assess surgical duration and surgical and post-operative complications associated with Video-Assisted Thoracoscopic Surgery (VATS) and lung biopsy in horses with severe Equine Pasture Asthma (EPA) and paired control horses. Twelve horses (6 EPA-affected, 6 control) were sex, age and breed matched. Twenty-four thoracic surgeries were performed. Surgery of each matched pair (EPA-affected and healthy) was performed during asthma exacerbation (summer) and remission (winter). Surgical times were shorter with uncomplicated thoracoscopy (85 min) and...
The effect of topical antibiotic or antibiotic-corticosteroid treatment on the ocular surface microbiota of healthy horses. Information regarding the impact of topical antibiotics with or without corticosteroids on the microbiota of the horses' eyes is limited. This study aimed to describe the bacterial ocular surface microbiota in healthy horses and evaluate the effect of topical antibiotics or antibiotic-corticosteroid medication on the ocular surface microbiota. Unassigned: This was a prospective, randomized, longitudinal, blinded study in which one eye of 12 horses was treated 3 times daily for 7 days with neomycin, polymyxin B and bacitracin ophthalmic ointment ( = 6) or neomycin, polymyxin B and dexametha...
Pegiviruses and Coronavirus: Biomolecular Prevalence and Phylogenetic Analysis of Strains Detected in Italian Horse Populations. Equestrian sports play a significant economic role in the horse industry. In recent years, numerous equine viruses have emerged, among which are equine Pegiviruses and the re-emerging Equine coronavirus (ECoV). These viruses are distributed globally and primarily cause subclinical infections with unknown morbidity, even if ECoV can occasionally induce febrile and diarrheic episodes. To broaden the data on the Italian equine population, a study was conducted to assess their prevalence in two distinct horse populations belonging to the Carabinieri Corps (CC) and the Italian Army (IA) of the Ital...
Innovative diagnostic strategies for equine habronemiasis: exploring molecular identification, gene expression, and oxidative stress markers. Equine habronemiasis, caused by Habronema (H.) muscae, H. microstoma, and Draschia megastoma, is a parasitic disease that presents in both gastric and cutaneous forms. Conventional diagnostic methods often lack sensitivity due to intermittent egg shedding and nonspecific clinical signs. This study aimed to enhance diagnostic accuracy by integrating molecular identification, oxidative stress profiling, and cytokine gene expression analysis. Methods: A total of 100 horses from a private farm in Giza, Egypt, were clinically examined for signs of habronemiasis. Fecal and skin samples were exami...
Seasonal elevation in equine adrenocorticotropic hormone occurs throughout the contiguous United States and is influenced by latitude and age. To determine whether seasonal variation in equine plasma ACTH concentrations is affected by latitude between 29.7°N and 45.0°N in the US. Unassigned: A cohort study was performed between June 2019 and May 2020 in which plasma ACTH was measured monthly by chemiluminescence immunoassay. The study involved 201 apparently healthy horses from 9 locations within the US classified into northern, central, and southern latitude cohorts. Data analysis was performed with 3 major objectives: (1) identify outlier ACTH concentrations and horses; (2) estimate ACTH upper reference limits to define expected ...
Evolution of in vitro antimicrobial resistance at an equine hospital over 4 decades. This study aimed to document antimicrobial resistance patterns of bacteria frequently isolated at a referral equine hospital between 2020 and 2022 and to compare these results to those of studies carried out in previous decades at the same reference center. Unassigned: Using the Kirby-Bauer method, 340 tests were completed on bacterial isolates and compared to 233, 255, and 396 tests carried out in 1986 to 1988, 1996 to 1998, and 2007 to 2013, respectively. Data were analyzed with tests for trends, followed by pairwise Fisher tests and Bonferroni corrections. Unassigned: Increasing resistanc...
Utility of Infrared Thermography for Monitoring of Surface Temperature Changes During Horses’ Work on Water Treadmill with an Artificial River System. Water treadmill (WT) exercise is used for horses' rehabilitation and training. Given that each training needs to be individualized for each horse, the goal is to assess whether infrared thermography (IRT) can serve as a non-invasive tool for daily monitoring of individual training and rehabilitation progress in horses undergoing WT exercise. Fifteen Polish Warmblood school horses were subjected to five WT sessions: dry treadmill, fetlock-depth water, fetlock-depth water with artificial river (AR), carpal-depth water, and carpal-depth water with AR. IRT images, collected pre- and post-exercise,...
Isolation, identification and comparative genomic analysis of Lactobacillus salivarius from Mongolian horse vagina. Reproductive health in mares is pivotal for the sustainability of the equine industry, yet vaginal microbiota dysbiosis remains an underrecognized contributor to infections such as endometritis and bacterial vaginosis. While spp. dominate healthy vaginal ecosystems in humans and livestock, their role in equine reproductive health, particularly in resilient breeds like Mongolian mares, is poorly understood. This study aimed to isolate and characterize a novel strain from the vaginal microbiota of healthy Mongolian mares and evaluate its probiotic potential for mitigating equine reproductive d...
Trigeminal Nerve Asymmetry in Horses With Idiopathic Trigeminal-Mediated Headshaking: A Retrospective Case-Control Magnetic Resonance Imaging Study. Nerve atrophy results in trigeminal nerve (TN) asymmetry detectable by magnetic resonance imaging (MRI) in humans, but similar studies have not been performed in horses with idiopathic trigeminal-mediated headshaking (ITMHS). Objective: Horses with ITMHS show greater MRI-detected trigeminal-nerve asymmetry than controls. Methods: A total of 20 adult horses with ITMHS and six unaffected control horses. Methods: Retrospective case-control study of the TN cross-sectional area (TNCSA) based on 3-Tesla MRI scans of the equine brain. TNCSA and its side-to-side differences at four defined measurement...
Does Low-Field MRI Tenography Improve the Detection of Naturally Occurring Manica Flexoria Tears in Horses? Diagnosing digital flexor tendon sheath (DFTS) pathologies, particularly manica flexoria (MF) tears, can be challenging with standard imaging modalities. Standing low-field MRI tenography (MRIt) may improve the detection rate of MF tears. This study aimed to compare ultrasonography, contrast radiography, pre-contrast MRI, and MRIt to detect naturally occurring MF lesions in horses undergoing tenoscopy. Ten horses with a positive DFTS block, which underwent contrast radiography, ultrasonography, MRI, MRIt, and tenoscopy were included. Two radiologists evaluated the images and recorded whether a...
Assessment of Salivary Biomarkers of Gastric Ulcer in Horses from a Clinical Perspective. This study arises from the search for non-invasive diagnostic alternatives for equine gastric ulceration (EGUS), which is prevalent, clinically variable and only confirmed by gastroscopy. The aim is to quantify five salivary biomarkers (IL1-F5, PIP, CA VI, serotransferrin, albumin) under clinical conditions by validated assays and analyse their diagnostic value. Horses were grouped in No EGUS (neither clinical signs of EGUS nor gastric lesions), EGUS non-clinical (apparently no clinical signs of EGUS but with gastric lesions), and EGUS clinical (obvious clinical signs of EGUS and with gastric ...
The use of systemic immune inflammatory index as a predictor for nematodes infections in horses. Nematode infections are a significant health concern in horses, causing a range of clinical signs and economic losses. Early detection and diagnosis are crucial for effective treatment and management. Unassigned: Examining the application of the systemic immune Inflammatory index (SII) as a predictor for nematode infections in horses, using platelets count, leucocytes count, and neutrophils count. Unassigned: A cross-sectional study was conducted on 164 horses, consisting of 66 horses with nematode infections and 98 horses without infections. The SII was computed using the platelets count, leu...
Description and Complications of a New Modified Semi-Closed Castration Technique in Horses. Castration remains a common surgical procedure in equids, yet postoperative complications such as hemorrhage, infection, and eventration persist, influencing surgical outcomes. This retrospective study introduced a novel modified semi-closed castration technique aimed at minimizing these complications. Eighty-five sexually intact mature male equids (including horses, ponies, and donkeys) underwent the described inguinal castration procedure under general anesthesia. The technique involved a carefully managed opening and subsequent secure closure of the parietal vaginal tunic, enabling direct i...
Alterations in the Microbiome of Horses Affected with Fecal Water Syndrome. Fecal water syndrome (FWS) in horses is characterized by two-phase defecation, including both solid and liquid phases. While satisfactory explanations for FWS are unavailable, bacterial dysbiosis has been suggested as a contributing or causative factor. The objectives of this study were to determine whether fecal bacterial dysbiosis is associated with FWS in horses in the midwestern USA. Fecal samples were collected from horses with FWS and from unaffected horses at the same location. In total, 16S rRNA amplicon libraries produced from fecal bacterial DNA were sequenced using the Illumina sequ...
Sedative effects of detomidine and midazolam combination in horses. The combination of detomidine and midazolam may provide safer sedation with muscle-relaxant effects, which are particularly necessary during standing procedures. Unassigned: Thus, the objective of this study was to evaluate the sedative and muscle-relaxant effects of the detomidine/midazolam combination in horses through a pharmacodynamic study. Unassigned: Five horses were used and subjected to four experimental protocols: 15 µg/kg of detomidine (D15); a combination of detomidine (15 µg/kg) with 25 µg/kg (DM25), 50 µg/kg (DM50), or 75 µg/kg (DM75) of midazolam administered intravenously....
Pioglitazone does not adequately control hyperinsulinemia but does increase serum adiponectin concentrations in equids with severe insulin dysregulation. To characterize the effects of pioglitazone (2 mg/kg, PO, either q 24 h [PIO-SID] or q 12 h [PIO-BID]) in equids with severe hyperinsulinemia. Unassigned: 17 client-owned equids with severe resting hyperinsulinemia (> 100 µIU/mL) were assigned to PIO-SID or PIO-BID in a prospective cohort study with rolling enrollment over an 11-month study period (December 13, 2022, to November 17, 2023). Basal insulin, total and high-molecular-weight adiponectin concentrations, and plasma pioglitazone concentrations were measured over 70 days. Oral sugar tests (OSTs) were performed on days 0 and 28. Results...