Analyze Diet

Topic:Animal Science

Animal Science and horses encompass the study of equine biology, physiology, and management practices aimed at understanding and improving horse health, welfare, and performance. This field integrates various scientific disciplines, including genetics, nutrition, reproduction, and behavior, to address the needs of horses in diverse contexts such as sports, work, and companionship. Research in this area often focuses on optimizing feeding strategies, enhancing breeding programs, and developing effective health management protocols. Additionally, studies explore the genetic factors influencing traits such as athleticism and disease resistance, as well as the impact of environmental and management conditions on horse behavior and welfare. This page gathers peer-reviewed research and scholarly articles that investigate the scientific principles underpinning equine science and their practical applications in horse care and management.
Ovarian and Uterine Dynamics During the Estrous Cycle in Criollo Breed Mares.
Journal of equine veterinary science    September 29, 2022   Volume 118 104131 doi: 10.1016/j.jevs.2022.104131
Duval LH, Rechsteiner SMF, Gastal GDA, Gastal MO, Mattos RC, Gastal EL.After having been brought to America 400 years ago, the Criollo horse reproduced freely for centuries in the southern part of the American continent. Roughness, resistance, and endurance are typical characteristics of this breed of horses. Although the natural selection that occurred over the centuries may have positively influenced the fertility and longevity of this breed, information regarding ovarian function and other reproductive aspects of Criollo breed mares under natural or controlled management conditions is scarce. The objectives of the present study were to (1) characterize the fol...
Endometrial and oviduct extra-cellular vescicles for in vitro equine sperm hyperactivation and oocyte fertilization.
Theriogenology    September 28, 2022   Volume 194 35-45 doi: 10.1016/j.theriogenology.2022.09.023
Lange-Consiglio A, Capra E, Giuliani D, Canesi S, Funghi F, Bosi G, Cretich M, Frigerio R, Galbiati V, Cremonesi F.Unlike humans and many other mammalian species, conventional in vitro fertilization (IVF) in equine species is not successful. To mimic in vitro equine spermatozoon-oviduct interaction as close as possible to that which occurs in vivo, extracellular vesicles (EVs) secreted by the female genital tract were used. Three female genital tracts were collected at slaughterhouse from mares in late estrus. Ipsilateral proximal and apical horn endometrial explants were digested with collagenase and trypsin and cells obtained were cultured on insert system to allow their polarization. Ipsilateral ovid...
A Novel A > G Polymorphism in the Intron 1 of LCORL Gene Is Significantly Associated with Hide Weight and Body Size in Dezhou Donkey.
Animals : an open access journal from MDPI    September 27, 2022   Volume 12, Issue 19 2581 doi: 10.3390/ani12192581
Wang T, Shi X, Liu Z, Ren W, Wang X, Huang B, Kou X, Liang H, Wang C, Chai W.Several studies have shown the association between the ligand-dependent nuclear receptor compression-like protein (LCORL) gene and body size in horses, pigs and donkeys. Based on previous studies, the LCORL gene was hypothesized to be associated with growth traits and hide weight in Dezhou donkeys. In this study, we aimed to reveal the variation of the LCORL gene in the Dezhou donkey and explore whether the gene is associated with hide weight and body size. In this study, genetic polymorphisms in the LCORL gene of the Dezhou donkey were studied using targeted sequencing technology, and single ...
Y Chromosome Haplotypes Enlighten Origin, Influence, and Breeding History of North African Barb Horses.
Animals : an open access journal from MDPI    September 27, 2022   Volume 12, Issue 19 doi: 10.3390/ani12192579
Radovic L, Remer V, Krcal C, Rigler D, Brem G, Rayane A, Driss K, Benamar M, Machmoum M, Piro M, Krischke D, Butler-Wemken IV, Wallner B.In horses, demographic patterns are complex due to historical migrations and eventful breeding histories. Particularly puzzling is the ancestry of the North African horse, a founding horse breed, shaped by numerous influences throughout history. A genetic marker particularly suitable to investigate the paternal demographic history of populations is the non-recombining male-specific region of the Y chromosome (MSY). Using a recently established horse MSY haplotype (HT) topology and KASP™ genotyping, we illustrate MSY HT spectra of 119 Barb and Arab-Barb males, collected from the Maghreb regio...
The major role of junctional diversity in the horse antibody repertoire.
Molecular immunology    September 27, 2022   Volume 151 231-241 doi: 10.1016/j.molimm.2022.09.011
Navas C, Manso T, Martins F, Minto L, Moreira R, Minozzo J, Antunes B, Vale A, McDaniel JR, Ippolito GC, Felicori LF.The antibody repertoire (Rep-seq) sequencing revolutionized the diversity of antigen B cell receptor studies, allowing deep and quantitative analysis to decipher the role of adaptive immunity in health and disease. Particularly, horse (Equus caballus) polyclonal antibodies have been produced and used since the century XIX to treat and prophylaxis diphtheria, tuberculosis, tetanus, pneumonia, and, more recently, COVID-19. However, our knowledge about the horse B cell receptors repertories is minimal. We present a deep horse antibody heavy chain repertoire (IGH) characterization of non-infected ...
Discovery of exercise-related genes and pathway analysis based on comparative genomes of Mongolian originated Abaga and Wushen horse.
Open life sciences    September 26, 2022   Volume 17, Issue 1 1269-1281 doi: 10.1515/biol-2022-0487
Pan J, Purev C, Zhao H, Zhang Z, Wang F, Wendoule N, Qi G, Liu Y, Zhou H.The Mongolian horses have excellent endurance and stress resistance to adapt to the cold and harsh plateau conditions. Intraspecific genetic diversity is mainly embodied in various genetic advantages of different branches of the Mongolian horse. Since people pay progressive attention to the athletic performance of horse, we expect to guide the exercise-oriented breeding of horses through genomics research. We obtained the clean data of 630,535,376,400 bp through the entire genome second-generation sequencing for the whole blood of four Abaga horses and ten Wushen horses. Based on the data an...
Unhandled horses classified with broken/unbroken test (BUT) exhibit longer avoidance, flight reactions, and displacement behaviors when approached by humans.
Frontiers in veterinary science    September 26, 2022   Volume 9 1022255 doi: 10.3389/fvets.2022.1022255
Riva MG, Sobrero L, Menchetti L, Minero M, Padalino B, Dalla Costa E.Horses with a low level of tameness are at higher risk for transport-related disease and injury; hence, European regulations for the protection of animals during transport (EC 1/2005) are stricter for unhandled (unbroken) horses. However, the regulation does not provide adequate tools for unhandled horse identification. The Broken/Unbroken Test (BUT) was developed and validated to easily identify whether a horse is broken (handled) or not. As a further validation step, the aim of this study was to assess whether there is any correspondence between the BUT classification and the behavioral resp...
Comparison of gut microflora of donkeys in high and low altitude areas.
Frontiers in microbiology    September 26, 2022   Volume 13 964799 doi: 10.3389/fmicb.2022.964799
Guo R, Zhang S, Chen J, Shen W, Zhang G, Wang J, Zhang F, Pan Q, Xie T, Ai D, Dong J, Suo J, Sun Y, Liu S.Donkeys' gut microbe is critical for their health and adaptation to the environment. Little research has been conducted on the donkey gut microbiome compared with other domestic animals. The Tibetan Plateau is an extreme environment. In this study, 6 Qinghai donkeys (QH) from the Tibetan Plateau and 6 Dezhou donkeys (DZ) were investigated, and the contents of 4 parts-stomach, small intestine, cecum, and rectum-were collected. 16S rRNA sequencing and metagenomic sequencing were used to analyze the composition and diversity of gut microbial communities in donkeys. The results showed that the flo...
Shallow shotgun sequencing of the microbiome recapitulates 16S amplicon results and provides functional insights.
Molecular ecology resources    September 26, 2022   Volume 23, Issue 3 549-564 doi: 10.1111/1755-0998.13713
Stothart MR, McLoughlin PD, Poissant J.Prevailing 16S rRNA gene-amplicon methods for characterizing the bacterial microbiome of wildlife are economical, but result in coarse taxonomic classifications, are subject to primer and 16S copy number biases, and do not allow for direct estimation of microbiome functional potential. While deep shotgun metagenomic sequencing can overcome many of these limitations, it is prohibitively expensive for large sample sets. Here we evaluated the ability of shallow shotgun metagenomic sequencing to characterize taxonomic and functional patterns in the faecal microbiome of a model population of feral ...
High-throughput sequencing technologies in the detection of livestock pathogens, diagnosis, and zoonotic surveillance.
Computational and structural biotechnology journal    September 26, 2022   Volume 20 5378-5392 doi: 10.1016/j.csbj.2022.09.028
Suminda GGD, Bhandari S, Won Y, Goutam U, Kanth Pulicherla K, Son YO, Ghosh M.Increasing globalization, agricultural intensification, urbanization, and climatic changes have resulted in a significant recent increase in emerging infectious zoonotic diseases. Zoonotic diseases are becoming more common, so innovative, effective, and integrative research is required to better understand their transmission, ecological implications, and dynamics at wildlife-human interfaces. High-throughput sequencing (HTS) methodologies have enormous potential for unraveling these contingencies and improving our understanding, but they are only now beginning to be realized in livestock resea...
‘Investing in people is key for our profession’.
The Veterinary record    September 24, 2022   Volume 191, Issue 6 244-246 doi: 10.1002/vetr.2253
Morley M, Loeb J.As Malcolm Morley becomes BVA president for 2022/23, Josh Loeb chats to him about his love for horses and why the veterinary profession is actually a human-centred business.
First isolation of verocytotoxin-producing Escherichia coli O157:H7 from sports animals in Southern Thailand.
Veterinary world    September 23, 2022   Volume 15, Issue 9 2275-2284 doi: 10.14202/vetworld.2022.2275-2284
Songsri J, Mala W, Wisessombat S, Siritham K, Cheha S, Noisa N, Wongtawan T, Klangbud WK. O157:H7 is enterohemorrhagic , which produces verocytotoxin or Shiga toxin. It is a well-known cause of severe diseases in humans worldwide. Cattle and other ruminants are the main reservoirs of this organism. Sports animals, such as fighting bulls, riding horses, and fighting cocks, are economic animals in Southern Thailand. This study aimed to identify O157:H7 from the rectal swabs of these sports animals and determine the antimicrobial susceptibility patterns of isolated bacteria. Unassigned: The rectal swabs were collected from 34 fighting bulls, 32 riding horses, and 31 fighting cocks. ...
Establishment of a reverse transcription real-time quantitative PCR method for Getah virus detection and its application for epidemiological investigation in Shandong, China.
Frontiers in microbiology    September 23, 2022   Volume 13 1009610 doi: 10.3389/fmicb.2022.1009610
Cao X, Qiu X, Shi N, Ha Z, Zhang H, Xie Y, Wang P, Zhu X, Zhao W, Zhao G, Jin N, Lu H.Getah virus (GETV) is a mosquito-borne, single-stranded, positive-sense RNA virus belonging to the genus of the family . Natural infections of GETV have been identified in a variety of vertebrate species, with pathogenicity mainly in swine, horses, bovines, and foxes. The increasing spectrum of infection and the characteristic causing abortions in pregnant animals pose a serious threat to public health and the livestock economy. Therefore, there is an urgent need to establish a method that can be used for epidemiological investigation in multiple animals. In this study, a real-time reverse tr...
Determination of hair cortisol in horses: comparison of immunoassay vs LC-HRMS/MS.
Analytical and bioanalytical chemistry    September 22, 2022   Volume 414, Issue 28 8093-8105 doi: 10.1007/s00216-022-04343-6
The measure of hair cortisol concentration (HCC) is becoming an emerging approach to monitor mid-/long-term stress in animals, so it is more and more important to develop accurate and reliable methods. In the light of this, the aim of the present study was to compare mane HCCs of 47 horses with different managements, by means of an immunoassay (ELISA) and liquid chromatography coupled to hybrid high-resolution mass spectrometry (LC-HRMS/MS). After the washing step, the ground hair was extracted with methanol. The extract was evaporated and redissolved in two different aqueous solutions, depend...
Undetectable vitamin D3 in equine skin irradiated with ultraviolet light.
Journal of equine science    September 21, 2022   Volume 33, Issue 3 45-49 doi: 10.1294/jes.33.45
Azarpeykan S, Gee EK, Thompson KG, Dittmer KE.Vitamin D requirements for most animals are expected to be fulfilled through daily exposure of the skin to solar ultraviolet B radiation. The synthesis of vitamin D in skin depends on different factors including melanin pigmentation, the amount of UVB radiation reaching the skin, type of clothing/hair coat, latitude and altitude, season, and time of day. Alternatively vitamin D may be obtained from UVB irradiated pasture species. Recent studies have shown that in unsupplemented grazing horses 25-hydroxyvitamin D is the predominant form of vitamin D in plasma, and that 25OHD is undetectable sug...
Metabolomic Profiles in Starved Light Breed Horses during the Refeeding Process.
Animals : an open access journal from MDPI    September 21, 2022   Volume 12, Issue 19 2527 doi: 10.3390/ani12192527
Main SC, Brown LP, Melvin KR, Campagna SR, Voy BH, Castro HF, Strickland LG, Hines MT, Jacobs RD, Gordon ME, Ivey JLZ.The large population of emaciated horses continues to be an issue troubling the equine industry. However, little is known regarding the collection of equine metabolites (metabolome) during a malnourished state and the changes that occur throughout nutritional rehabilitation. In this study, ten emaciated horses underwent a refeeding process, during which blood samples were collected for a blood chemistry panel and metabolomics analysis via ultrahigh performance liquid chromatography-high resolution mass spectrometry (UHPLC-HRMS). Significant differences among blood chemistry analytes and metabo...
Basal Reactivity Evaluated by Infrared Thermography in the “Caballo de Deporte Español” Horse Breed According to Its Coat Color.
Animals : an open access journal from MDPI    September 21, 2022   Volume 12, Issue 19 doi: 10.3390/ani12192515
Bartolomé E, Perdomo-González DI, Ripollés-Lobo M, Valera M.Horses have been valued for their diversity of coat color since prehistoric times. In particular, the pleiotropic effect that coat color genes have on behavior determines the way the horse perceives and reacts to its environment. The primary aim of this study was to evaluate the influence of coat color on basal reactivity assessed with infrared thermography as eye temperature at rest (ETR), determine their relation with the results obtained by these horses in Show Jumping competitions and to estimate the genetic parameters for this variable to test its suitability for genetic selection. A Gene...
Videodermoscopic examination of the skin and its products in purebred Arabian horses in the summer season.
Journal of equine science    September 21, 2022   Volume 33, Issue 3 37-44 doi: 10.1294/jes.33.37
Pomorska-Zniszczyńska A, Szczepanik M, Adamczyk N, Tarach M.Videodermoscopy is a method that enables the examination of many parameters of the skin and its structures. The aim of this study was to assess specific dermoscopic parameters in purebred Arabian horses during the summer. The study involved 21 clinically healthy purebred Arabian horses (18 mares and three stallions) that had not been used for breeding and were 1 to 25 years old. The videoderoscopic evaluation was performed on seven selected areas of the body: forehead, mane, neck, chest, flank, rump, and tail. The tests were carried out with Vidix and Olympus cellSens specialised software. Vid...
Effect of Cooling Blanket on the Heat Stress of Horses in Hot and Humid Environments.
Animals : an open access journal from MDPI    September 20, 2022   Volume 12, Issue 19 2505 doi: 10.3390/ani12192505
Ojima Y, Torii S, Maeda Y, Matsuura A.Heat stress is serious problem for livestock. While riding horses and racehorses spend the majority of their days in stalls, there are few reports on effective methods for reducing stall heat stress. The aim of the present study was to evaluate the effectiveness of an ice horse blanket in hot and humid environments. Twenty healthy horses were measured first without the blanket (C) and then measured with the blanket (IB), or vice versa, in a cross-over trial. The blanket was designed to keep cooling the front back, the rear back, and the loin. Skin surface temperature, respiratory rate, rectal ...
Non-native grazers affect physiological and demographic responses of greater sage-grouse.
Ecology and evolution    September 20, 2022   Volume 12, Issue 9 e9325 doi: 10.1002/ece3.9325
Behnke T, Street P, Davies S, Ouyang JQ, Sedinger JS.Non-native ungulate grazing has negatively impacted native species across the globe, leading to massive loss of biodiversity and ecosystem services. Despite their pervasiveness, interactions between non-native grazers and native species are not fully understood. We often observe declines in demography or survival of these native species, but lack understanding about the mechanisms underlying these declines. Physiological stress represents one mechanism of (mal)adaptation, but data are sparse. We investigated glucocorticoid levels in a native avian herbivore exposed to different intensities of ...
Trichinella spiralis and T. britovi in North-Eastern Romania: A Six-Year Retrospective Multicentric Survey.
Veterinary sciences    September 17, 2022   Volume 9, Issue 9 509 doi: 10.3390/vetsci9090509
The genus includes species with a wide geographical spread that cause pathology in humans and animals. In this context, an epidemiological study of infection was carried out in the northeastern part of Romania to investigate for the first time the prevalence of this infection in pigs, horses, wild boars and bears, the geographical distribution of species and the natural reservoir of infection. Between 2010 and 2015, a total of 166,270 animals were examined by the method of artificial digestion, in order to calculate the annual and general prevalence of infection, according to the host and...
Overnight holding aids in selection of developmentally competent equine oocytes.
Animal reproduction science    September 17, 2022   Volume 245 107071 doi: 10.1016/j.anireprosci.2022.107071
Merlo B, Del Prete C, Mari G, Iacono E.The demand for equine in vitro produced embryos has increased over the last decade. The aim of this study was to compare the effects of an extended IVM or a prolonged period before fertilization, including holding time, on equine immature oocyte developmental competence. Oocytes, collected from abattoir-derived ovaries, were divided into 4 groups: H0/24 (n = 165) 0 h holding + standard 24-26 h IVM; H8/36 (n = 160) 8 h holding + 36 h IVM; H20/24 (n = 187) 20 h holding + 24 h IVM; H0/44 (n = 164) 0 h holding + 44 h IVM. Oocytes matured to MII were fertilized by intracytoplasm...
B-mode and color Doppler ultrasonography of normal external jugular vein in donkeys (Equus asinus).
BMC veterinary research    September 14, 2022   Volume 18, Issue 1 345 doi: 10.1186/s12917-022-03441-y
Hussein HA, Ibrahim A.Although the jugular vein is a major important blood vessel in equine, the literature lacks this vessel's normal B-mode and Doppler ultrasonographic examinations in donkeys. Therefore, this study aimed to determine the reference ranges of B-mode and Doppler ultrasonographic indices of jugular veins in healthy adult donkeys (Equus asinus) and the possible effect of examination side (left and right), gender, and body condition on the ultrasonographic measurements of this vessel. B-mode and Doppler ultrasound imaging of the external jugular vein was conducted on 20 adult healthy donkeys of both s...
The Welfare of Traveller and Gypsy Owned Horses in the UK and Ireland.
Animals : an open access journal from MDPI    September 13, 2022   Volume 12, Issue 18 2402 doi: 10.3390/ani12182402
Rowland M, Hudson N, Connor M, Dwyer C, Coombs T.Travellers and Gypsies are recognised ethnic groups in the UK and Ireland. Horse ownership is an important cultural tradition, however, practices associated with poor welfare are often perceived to be linked to these horse owning communities. Despite this, empirical studies on the welfare status of Traveller and Gypsy owned horses are lacking. To determine the welfare status of Traveller and Gypsy owned horses, 104 horses were assessed using a bespoke horse welfare protocol. This protocol assessed animal, resource and management-based measures. In addition, Qualitative Behaviour Assessment (QB...
Facial Expressions of Horses Using Weighted Multivariate Statistics for Assessment of Subtle Local Pain Induced by Polylactide-Based Polymers Implanted Subcutaneously.
Animals : an open access journal from MDPI    September 13, 2022   Volume 12, Issue 18 2400 doi: 10.3390/ani12182400
Carvalho JRG, Trindade PHE, Conde G, Antonioli ML, Funnicelli MIG, Dias PP, Canola PA, Chinelatto MA, Ferraz GC.Facial-expression-based analysis has been widely applied as a pain coding system in horses. Herein, we aimed to identify pain in horses undergoing subcutaneously polylactide-based polymer implantation. The sham group was submitted only to surgical incision. The horses were filmed before and 24 and 48 h after implantation. Five statistical methods for evaluating their facial expressions (FEs) were tested. Primarily, three levels of scores (0, 1, and 2) were applied to the seven FEs (ear movements, eyebrow tension, orbicularis tension, dilated nostrils, eye opening, muzzle tension, and masticato...
Combined Antenna-Channel Modeling for the Harsh Horse Hoof Environment.
Sensors (Basel, Switzerland)    September 10, 2022   Volume 22, Issue 18 6856 doi: 10.3390/s22186856
Goethals J, Nikolayev D, Thielens A, Vermeeren G, Verloock L, Deruyck M, Martens L, Joseph W.This paper describes the exploration of the combined antenna-channel model for a horse hoof. An antenna of 25 mm × 40 mm is designed in the ISM 868 MHz band. During the characterization and design of the antenna, the dynamic and harsh environment of the horse hoof is taken into account throughout every step of the procedure because it is impossible to de-embed the antenna from its environment. The antenna and channel model are verified extensively by measurements in phantom and ex vivo. The antenna is verified to be robust against changes in the morphology of the horse's hoof up to 50%. The d...
What is your diagnosis? Ovarian mass in a mare.
Veterinary clinical pathology    September 9, 2022   Volume 52 Suppl 2 134-137 doi: 10.1111/vcp.13161
Parsley AL, Rasche BL, Yang TS, Gruber EJ.No abstract available
Prevalence of brucellosis in livestock of African and Asian continents: A systematic review and meta-analysis.
Frontiers in veterinary science    September 9, 2022   Volume 9 923657 doi: 10.3389/fvets.2022.923657
Suresh KP, Patil SS, Nayak A, Dhanze H, Rajamani S, Shivamallu C, Cull CA, Amachawadi RG.Brucellosis is a highly contagious bacterial disease that mainly affects ruminants, but it may affect equines, canines, and felines. The disease is of utmost significance from an economic standpoint in countries where there is no national brucellosis prevention and eradication policy in operation. A systematic review was done to estimate disease burden, incidences, prevalence, and geographical distribution critical in planning appropriate intervention strategies for the control and prevention of Brucellosis. Research articles that were published during the period 2000-2020 were considered for ...
Gut health of horses: effects of high fibre vs high starch diet on histological and morphometrical parameters.
BMC veterinary research    September 8, 2022   Volume 18, Issue 1 338 doi: 10.1186/s12917-022-03433-y
Colombino E, Raspa F, Perotti M, Bergero D, Vervuert I, Valle E, Capucchio MT.The conventional feeding management of horses is still characterized by high starch and low fibre diets, which can negatively affect horse's gastrointestinal health. Thus, the aim of this study was to compare the effects of a high-starch (HS) vs. a high-fibre (HF) diet on gut health in horses. A total of 19 Bardigiano horses destined for slaughter and aged 14.3 ± 0.7 months were randomly allotted to two dietary groups: HS (5 fillies and 4 colts,) and HF group (7 fillies and 3 colts). They received the same first-cut meadow hay but different complementary feeds for 72 days: HS group was ...
Welfare of equidae during transport.
EFSA journal. European Food Safety Authority    September 7, 2022   Volume 20, Issue 9 e07444 doi: 10.2903/j.efsa.2022.7444
Nielsen SS, Alvarez J, Bicout DJ, Calistri P, Canali E, Drewe JA, Garin-Bastuji B, Gonzales Rojas JL, Gortázar Schmidt C, Michel V....In the framework of its Farm to Fork Strategy, the Commission is undertaking a comprehensive evaluation of animal welfare legislation. This opinion deals with the protection of horses and donkeys during transport. While the opinion focuses primarily on road transport of horses, there are specific sections dealing with the transport of horses on roll-on-roll-off ferries, horses transported by air and the transport of donkeys. In addition, the opinion covers welfare concerns in relation to a specific scenario identified by the European Commission related to the transport of horses on long journe...
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