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Topic:Animal Species

The topic "Animal Species and Horses" explores the interactions and relationships between horses and various other animal species. This includes understanding the ecological roles horses play within their environments, as well as their interactions with domestic and wild animals. Research in this area may cover aspects such as competition for resources, symbiotic relationships, and behavioral influences between horses and other species. Studies may also investigate the impact of horses on biodiversity and ecosystem dynamics. This page compiles peer-reviewed research studies and scholarly articles that examine these interspecies relationships, focusing on the ecological, behavioral, and environmental implications of horses interacting with other animals.
Using STR Data to Investigate the Impact of the Studbook Cap on Genetic Diversity in the American Standardbred Horse from 1998 to 2021.
Genes    June 27, 2025   Volume 16, Issue 7 748 doi: 10.3390/genes16070748
Avila F, Esdaile E, Bellone RR. Standardbreds, a breed of horses used in harness racing at either the trot or the pace, established a closed studbook in 1973. Concerns about genetic diversity within the breed led the United States Trotting Association (USTA) to establish a limit of mares bred per stallion (i.e., a studbook cap) in 2009. Here, we aimed to evaluate the impact of the breeding restrictions on genetic diversity between and among subpopulations. : Sixteen short tandem repeats (STRs) were analyzed across a dataset of 176,424 Standardbreds foaled in the United States between 1998 and 2021. We examined allelic richn...
Multisystemic Disease and Septicemia Caused by Presumptive Burkholderia pseudomallei in American Quarter Horse, Florida, USA.
Emerging infectious diseases    June 26, 2025   Volume 31, Issue 7 1432-1436 doi: 10.3201/eid3107.241009
Thornton JJ, Roberts JF, AuCoin DP, Tuanyok A.We report a presumptive case of melioidosis caused by an atypical Burkholderia pseudomallei serotype in an American quarter horse in north-central Florida, USA, through archived formalin-fixed paraffin-embedded specimens dating back to 2006. This case underscores the potential pathologic impact of emergent B. pseudomallei in the Gulf region of the United States.
Detection of Novel Orthobunyavirus Reassortants in Fatal Neurologic Case in Horse and Culicoides Biting Midges, South Africa.
Emerging infectious diseases    June 26, 2025   Volume 31, Issue 7 1455-1459 doi: 10.3201/eid3107.241800
Rakaki ME, van der Walt M, Williams J, Venter M.We detected Shuni virus in horses and ovine fetuses and Shamonda virus in a caprine fetus in South Africa. We identified a Shuni/Shamonda virus reassortant in a horse and Shuni/Caimito, Shamonda/Caimito, and Shamonda/Sango virus reassortants in Culicoides midges. Continued genomic surveillance will be needed to detect orthobunyavirus infections in Africa.
Tracheal collapse rates in Kenyan working cart and pack donkeys.
Equine veterinary journal    June 26, 2025   doi: 10.1111/evj.14545
Mellish M, Burns J, Elce Y, Stull JW.Donkeys (Equus asinus) in East Africa are recognised as working with poorly designed harnesses and carts. The donkey cart used in regions of Africa, specifically Meru County, Kenya, appears to place the cart's weight solely on the donkey's mid-cervical region. The ventral area of the neck is vulnerable to external pressure on the trachea, which is superficially located. Objective: To compare the presence and severity of endoscopic abnormalities in the upper airway and trachea of Meru County working cart donkeys compared to pack donkeys. To determine associations between endoscopic and physical...
A comparison of the efficacy of three commercial human embryo vitrification kits for cryopreservation of in vivo produced equine embryos.
Equine veterinary journal    June 26, 2025   doi: 10.1111/evj.14539
Wilsher S, Ismer A, Grippo A, Hoogewijs M, Bussade P, Kovacsy S.Different cryoprotectants can influence the ability of embryos to successfully survive vitrification and subsequent warming before transfer. Objective: To compare pregnancy rates for embryos ≤500 μm vitrified, without puncture or aspiration of the blastocoele cavity, with one of three commercial human embryo vitrification kits containing the same penetrating cryoprotectants (DMSO and EG) but varying in their non-penetrating cryoprotectants (NPCPAs; sucrose, trehalose, dextran serum supplement [DSS], and hydroxypropyl cellulose [HPC]). Methods: In vivo experiments. Methods: Embryos (n =...
Comparison of early and late time-controlled adaptive ventilation on pulmonary gas exchange in anesthetized horses.
Veterinary anaesthesia and analgesia    June 25, 2025   S1467-2987(25)00138-2 doi: 10.1016/j.vaa.2025.06.007
Vicenti C, Cercone M, Nieman G, Habashi NM, Andrews P, Satalin J, Velarde P, Mitchell KJ, Louie EW, Albano V, Ali H, King A, Staffieri F, Gleed R....To compare early versus late time-controlled adaptive ventilation (TCAV) for setting airway pressure release ventilation (APRV) on arterial oxygenation in dorsally recumbent anesthetized horses. Methods: A crossover, nonrandomized, experimental study. Methods: Six healthy adult horses. Methods: Each horse underwent two anesthetics: 1) volume-controlled ventilation (VCV) began for 30 minutes before switching to APRV using the TCAV method (late TCAV, TCAV); and 2) ventilation began immediately with TCAV (early TCAV, TCAV). The study lasted 180 minutes, with arterial blood gases and respiratory m...
Stress responses of horses transported long-distance by road to and from a jumping competition in a tropical climate.
Journal of animal science    June 24, 2025   skaf214 doi: 10.1093/jas/skaf214
Sanigavatee K, Wonghanchao T, Poochipakorn C, Pongnarudech P, Suchairat T, Jitsopin S, Wanichayanon B, Sripiboon S, Chanda M.Long-distance road transport is known to be stressful for horses, with most research conducted in temperate climates. This study investigated horses' stress responses during long-distance transport before and after a jumping competition in a tropical environment. Ten healthy horses (aged 7.9 ± 1.9 years) were studied. Blood cortisol concentrations, haematology, and heart rate variability (HRV) were monitored during outbound (OTJ) and inbound journeys (INJ). Cortisol concentrations increased five minutes after unloading and remained elevated for up to 180 minutes in the OTJ (P < 0.05-0.001)...
Effectiveness of steam cleaning technology on reducing the occurrence of ESKAPE organisms and Escherichia coli in the stables of an equine referral hospital.
Journal of equine veterinary science    June 21, 2025   Volume 151 105636 doi: 10.1016/j.jevs.2025.105636
Sosa-Portugal S, Dale L, Devaney J, Sharp A, Malalana F, Timofte D.The "ESKAPE" bacteria include a group of organisms known for their multidrug resistance and potential association with nosocomial infections in human and veterinary hospitals. Objective: This study aimed to evaluate the effectiveness of steam cleaning in reducing the number of ESKAPE organisms on environmental surfaces from the stables of an equine veterinary referral hospital. Methods: Environmental sampling was performed at two timepoints (pre- and post-steam cleaning) on smooth metallic doors and rough rubber stable walls. Microbiological culture targeted ESKAPE organisms and Escherichia co...
Trypanosoma evansi in Brazil: first evidence of infection in equines from Northeast region. Sousa JA, Miranda LM, Coutinho DJB, Costa TF, Costa SP, Freitas ÚS, Costa FB, Machado RZ, Nogueira RMS, Costa APD.The hemoprotozoan Trypanosoma evansi is a parasite that infects mammals, causing an infection known as trypanosomiasis. There is no report of T. evansi in horses in the State of Maranhão, highlighting the need to assess exposure and infection by the parasite and generate data for its monitoring. The objectives of this study were to identify T. evansi in blood samples from horses, investigate its occurrence in horses in this region, and analyze the associated risk factors. Three hundred blood samples were collected for parasitological (blood smear), serological (indirect enzyme-linked immunoso...
Equine sarcoids from Southern Italy: Molecular and Histopathological characterization.
Research in veterinary science    June 19, 2025   Volume 193 105777 doi: 10.1016/j.rvsc.2025.105777
Martano M, Montagnaro S, Power K, Cuccaro B, Cutarelli A, Maiolino P, Restucci B.This study investigated the presence of δ bovine papillomaviruses (BPV-1, BPV-2, BPV-13, BPV-14) in equine skin lesions from Southern Italy, focusing on equine sarcoids and their histopathological correlations. 63 equine skin samples were analysed using PCR and sequencing for BPV detection, and their histopathological features were assessed. BPV DNA was detected in 69.84% of the samples, with BPV-1 and BPV-2 being the most prevalent genotype, followed by BPV-13, while BPV-14 was not detected. BPV DNA was also found in non-sarcoid tumours and non-neoplastic conditions. Histopathological analys...
Haplotype structure and heterozygosity around the fragile foal syndrome variant in Swedish Warmblod horses.
Animal genetics    June 17, 2025   Volume 56, Issue 3 e70022 doi: 10.1111/age.70022
Ablondi M, Eriksson S, Mikko S.Fragile foal syndrome (FFS) is a disease caused by a recessive lethal missense mutation in the PLOD1 gene located on ECA2. Despite its harmful effect, a relatively high frequency of FFS carriers was observed in Warmblood breeds spanning from 7.4% in a random sample of Swedish Warmblood breed to 17% in the Hanoverian and Danish Warmblood, indicating potential heterozygous advantage. Balancing selection can be further studied based on haplotype blocks and via detection of heterozygosity-rich region (ROHet) around the target of selection. In this study we evaluated the presence of haplotype block...
Clinical evaluation of freeze-dried secretome (lyosecretome) for osteoarthritis: a controlled trial in dogs and preliminary safety assessment in horses.
International journal of pharmaceutics    June 16, 2025   Volume 681 125864 doi: 10.1016/j.ijpharm.2025.125864
Berni P, Del Bue M, Conti V, Andreoli V, Ramoni R, Angelone M, Squassino GP, Bari E, Torre ML, Rinaldi M, Dotti S, Rossi R, Yusuf I, Mauri P....Most in vivo studies on MSC-secretome for osteoarthritis (OA) have relied on animal models, using products lacking pharmaceutical quality, not formulated for clinical use, and insufficiently characterized, limiting knowledge of its effectiveness. This study reports veterinary clinical trials on dogs and horses with spontaneous OA: in dogs (26 subjects), the trial is randomized, double-blinded, and controlled; in horses, 5 clinical cases were treated for safety assessment. Treatment consisted of hyaluronic acid with either lyosecretome - a freeze-dried, injectable MSC-secretome obtained through...
Equine botulism.
Equine veterinary journal    June 15, 2025   doi: 10.1111/evj.14542
Slavik K, Whitlock R, Johnson A.Botulism is a severe and often fatal disease in equine patients worldwide. Clostridium botulinum is a ubiquitous soil organism which produces a potent neurotoxin resulting in neuromuscular blockade and flaccid paralysis in affected animals. Definitive diagnosis is often impractical or impossible, leading to diagnosis and treatment based on clinical experience and presenting signs. Delay in case identification and treatment results in rapid deterioration of the patient. Treatment of recumbent equine botulism cases presents challenges due to patient size and requires intensive nursing care. This...
Molecular detection of the Escherichia coli heme-utilization gene A virulence factor in E. coli isolated from the feces of horses in Sumbawa island, Indonesia.
Veterinary research forum : an international quarterly journal    June 15, 2025   Volume 16, Issue 6 325-330 doi: 10.30466/vrf.2024.2039823.4421
Kholik K, Sukri A, Priscilia Riwu KH, Ayu IW, Dewi IN.The transmission of () containing virulent genes from animals to humans and the environment poses significant public health challenges. This study aimed to detect the virulence factor of the () in isolated from the feces of apparently healthy horses in the island of Sumbawa, Indonesia. The study utilized 52 fecal samples from a total horse population of 283, calculated using the disease detection formula. Fresh feces were collected immediately after excretion and placed in buffered peptone water for subsequent analysis. The samples were then isolated on eosin methylene blue media and identi...
Cellular and Matrix Dynamics of the Equine Tendon.
The Veterinary clinics of North America. Equine practice    June 14, 2025   S0749-0739(25)00025-2 doi: 10.1016/j.cveq.2025.04.007
Koch DW.Our understanding of tendon homeostasis and repair following injury has made great strides through basic science, clinical veterinary medicine, and preclinical translational research. We have now gleaned a greater understanding of the cellular and matrix dynamics that are being orchestrated within the tendon, which provide therapeutic opportunities. This article will focus on how utilizing the horse and equine tissues have advanced our understanding of tendinopathy and the cellular and matrix dynamics at play while also identifying continued gaps in our knowledge where the horse as a veterinar...
Adjunct Therapies for Tendon/Ligament Healing: Therapeutic Laser and Extracorporeal Shockwave Therapy.
The Veterinary clinics of North America. Equine practice    June 14, 2025   Volume 41, Issue 2 391-401 doi: 10.1016/j.cveq.2025.04.009
Pluim M, Reynolds A, McClure S.High-power laser therapy and extracorporeal shock wave therapy (ESWT) can be safely used to treat tendon and ligament injuries in horses, and the potential beneficial effects have been described. The power of low level lasers is not sufficient for treatment of tendon and ligament injuries in horses. ESWT is used more often to treat tendon and ligament injuries than it is for any other type of condition in horses, but there is limited supporting scientific evidence. Further research should focus on establishing dosage and treatment protocols for both laser therapy and ESWT in horses with clinic...
Current and Emerging Biologic Therapies for Equine Tendon and Ligament Injuries.
The Veterinary clinics of North America. Equine practice    June 14, 2025   Volume 41, Issue 2 371-389 doi: 10.1016/j.cveq.2025.04.008
Connard SS, Schnabel LV.This review explores the use of biologics in the treatment of tendon and ligament injuries in horses, focusing on current and emerging therapies aimed at enhancing tissue repair and regeneration. It discusses the role of scaffold-based therapies, growth factors, blood-derived and tissue-derived biologics, and gene therapy as biologic regenerative therapies for improving healing outcomes. The review evaluates the mechanisms, efficacy, and challenges of biologics in clinical applications, highlighting recent advances and clinical studies. It provides an overview of current trends and future dire...
[Off-label use of antibiotics in equine medicine – an online survey in Germany].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    June 13, 2025   Volume 53, Issue 3 155-168 doi: 10.1055/a-2585-3269
Tarillion M, Hertzsch R, Richter A.Since 28.01.2022, veterinary medicinal products must be used in accordance with the conditions of authorization (Regulation (EU) 2019/6, Article 106). This entails further restrictions on therapeutic freedom, e. g., with regard to dose deviations. To date, there is no data available in equine medicine on the scope of off-label use as any use deviating from the expert information for antibiotics, on the basis of which the feasibility of implementing the new regulations in Germany can be assessed.To collect data on the off-label use of antibiotics, a Germany-wide anonymous online survey was th...
Catalase in Unexpected Places: Revisiting H2O2 Detoxification Pathways in Stallion Spermatozoa.
Antioxidants (Basel, Switzerland)    June 12, 2025   Volume 14, Issue 6 doi: 10.3390/antiox14060718
Medica AJ, Swegen A, Seifi-Jamadi A, McIntosh K, Gibb Z.Oxidative stress plays a critical role in regulating sperm function, yet species-specific antioxidant mechanisms remain poorly understood. This study compared hydrogen peroxide (H2O2) tolerance in horse and human sperm and investigated the roles of catalase and glutathione peroxidase (GPx) in horses. A H2O2 dose-response assay (0-2000 µM) showed that horse sperm were significantly more resistant to oxidative damage, with an IC50 for progressive motility over 14-fold higher than that of human sperm (391.6 µM vs. 27.3 µM). Horse sperm also accumulated more intracellular H2O2 without loss of m...
DNA Methylation of Igf2r Promoter CpG Island 2 Governs Cis-Acting Inheritance and Gene Dosage in Equine Hybrids.
Biology    June 11, 2025   Volume 14, Issue 6 678 doi: 10.3390/biology14060678
Wang X, Shen Y, Ren H, Yi M, Bou G.Genomic imprinting is critical for mammalian development, but its regulation varies across species. The insulin-like growth factor 2 receptor (IGF2R), which is a maternally expressed imprinted gene critical for cell proliferation and differentiation, as well as embryonic and placental development, is classically regulated by differentially methylated regions (DMRs) and lncRNA- in mice. However, studies on this in equus are scarce, especially in terms of mechanistic studies. In the present study, heart, liver, spleen, lung, kidney, brain, and muscle samples were obtained from horses, donkeys, a...
Epidemiology of Infectious Pathogens in Horses with Acute Respiratory Disease, Abortion, and Neurological Signs: Insights Gained from the Veterinary Surveillance System for Horses in The Netherlands (SEIN).
Veterinary sciences    June 10, 2025   Volume 12, Issue 6 567 doi: 10.3390/vetsci12060567
van Maanen K, van den Wollenberg L, de Haan T, Frippiat T.Monitoring infectious diseases is essential for safeguarding equine health and ensuring the sustainability of the horse industry. In 2019, the Royal Veterinary Association of the Netherlands (KNMvD) and Royal GD (GD Animal Health) launched SEIN (Surveillance of Equine Infectious diseases in the Netherlands), a voluntary surveillance system for laboratory-confirmed outbreaks of equid alphaherpesvirus 1 (EHV-1), equid alphaherpesvirus 4 (EHV-4), equine influenza virus (EIV), and subsp. . This retrospective study analyzed 364 confirmed outbreaks reported through SEIN between June 2019 and April ...
A single session of whole-body vibration did not affect cardiovascular autonomic recovery after a high intensity exercise in horses.
Journal of equine veterinary science    June 9, 2025   Volume 151 105631 doi: 10.1016/j.jevs.2025.105631
Sales NAA, Carvalho JRG, Littiere TO, Costa GB, Silva ACY, Rodriguez IDM, Castro CM, Anjos LS, Ottati ACM, Alcaide JA, Ramos GV, Ferraudo AS....Whole-body vibration (WBV) has the potential to enhance post-exercise recovery in humans. Heart rate variability (HRV), a well-established biomarker of cardiac autonomic regulation (CAR), provides insight into autonomic reactivity during recovery from exercise. Objective: To investigate CAR in healthy horses during post-exercise recovery following an acute intensive exercise bout (AIEB) with WBV intervention. Methods: A single AIEB was prescribed at velocities corresponding to lactate threshold (VLT), and the eight horses performed on treadmill with 5 % slope, 2 min at 110 % VLT followed by 3 ...
Review of published research on primary dysautonomia of domestic animals.
The Veterinary record    June 7, 2025   e5499 doi: 10.1002/vetr.5499
Harte T, Smith D, Moore J, Wells B.An article published in 1992 by Marion M. Pollin and I.R. Griffiths reviewed the topic of primary dysautonomias of the autonomic nervous system of unidentified origin in multiple domestic species, specifically cats, dogs and horses. Thirty-one years later, we appear no closer to identifying the causal agents of these strikingly similar diseases. This article reviews the similarities and differences in clinical presentation and pathology of dysautonomia in canids, felids, equids, leporids and other less frequently reported species.
Comparative clinical, virological and pathological characterization of equine rotavirus A G3P[12] and G14P[12] infection in neonatal mice.
The Journal of general virology    June 5, 2025   Volume 106, Issue 6 002110 doi: 10.1099/jgv.0.002110
Gamage C, Holl W, Parreño V, Thieulent CJ, Balasuriya UBR, Vissani MA, Barrandeguy ME, Carossino M.Group A rotavirus (RVA) infections are a leading cause of neonatal diarrhoea in foals. Neonatal mice could serve as a useful tool to study the pathogenesis of equine RVA (ERVA) as well as a preclinical model for assessment of vaccine efficacy. This study aimed to comparatively evaluate the clinical, virological and pathological features of ERVA G3P[12] and G14P[12] infection in neonatal mice and compare them with porcine OSU G5P[7] and bovine UK G6P[5] RVA reference strains. Neonatal mice orally inoculated with equine, bovine and porcine RVA developed short-lived diarrhoea at variable rates, G...
Stallion spermatozoa express LDH isoforms A, B, and C, with LDHC playing a crucial role in sustaining sperm viability.
Reproduction (Cambridge, England)    June 5, 2025   Volume 170, Issue 1 e240436 doi: 10.1530/REP-24-0436
Becerro-Rey L, Martín-Cano FE, Silva-Rodríguez A, Ortega-Ferrusola C, da Silva-Álvarez E, Ortiz-Placín C, Tapia JA, Gil MC, Peña FJ.Three isoforms of lactate dehydrogenase (LDH) - LDHA (cytoplasmic), LDHB (mitochondrial), and LDHC (flagellar) - have been identified and localized in stallion spermatozoa. Functional inhibition assays indicate that these three isoforms constitute a lactate shuttle of crucial importance for sperm function. Unassigned: Stallion spermatozoa use different energy sources; while oxidative phosphorylation predominates, glycolysis and beta-oxidation of fatty acids are also present. Glycolysis depends on the availability of NAD+ as an electron acceptor. During glycolysis, NAD+ is reduced to NADH. To e...
Does gradient isolation preserve equine neutrophil viability and oxidative function for up to six hours?
Veterinary immunology and immunopathology    May 31, 2025   Volume 285 110961 doi: 10.1016/j.vetimm.2025.110961
Alves LSS, Hasuda AL, Giordano LGP, Frederico I, Dos Santos IH, Pereira PFV, Lisbôa JAN, Flaiban KKMDC.This study investigated isolation of equine neutrophils and the assessment of their antioxidant function using the nitroblue tetrazolium (NBT) reduction test, an indicator of reactive oxygen species (ROS) activation. The objective was to test the viability of neutrophils isolated from equine whole blood and their ability to undergo respiratory burst, comparing the results with whole blood. The hypothesis tested was that isolation of equine neutrophils allows for effective functional evaluation, even after isolation process, with no significant differences between whole blood and isolated cells...
First detection of West Nile virus seropositivity in horses in southern Iraq.
Open veterinary journal    May 31, 2025   Volume 15, Issue 5 2016-2020 doi: 10.5455/OVJ.2025.v15.i5.17
Al-Rammahi HM, Mohsen RK, Othman RM.West Nile virus (WNV) circulates between birds and mosquitoes, with horses and humans as incidental dead-end hosts. Despite documented cases in neighboring countries and human cases in southern Iraq, no previous studies have investigated WNV in Iraqi horses. Unassigned: To determine the seroprevalence of WNV in horses from three southern Iraqi governorates (Thi-Qar, Misan, and Basrah) between March and June 2024. Unassigned: Blood samples were collected from 236 horses regardless of age, sex, or health status. Serum samples were analyzed using commercial competitive enzyme-linked immunosorbent...
Knowledge levels regarding antibiotics and their use among horse owners in the State of Rio Grande do Norte, Brazil.
Brazilian journal of veterinary medicine    May 30, 2025   Volume 47 e000425 doi: 10.29374/2527-2179.bjvm000425
Araújo IRDS, de Brito EL, de Melo UP, Mariz ALB, Cavalheiro MT, Ferreira C, de Morais LF, de Souza RF.This study aimed to evaluate the use of antimicrobials in horses, focusing on administration practices, adherence to veterinary prescriptions, and the impact on the development of antimicrobial resistance. The research was based on a questionnaire completed by 220 horse owners in the State of Rio Grande do Norte, Brazil. The data collected covered aspects including antibiotic use, prescription sources, the performance of culture and susceptibility tests, and the disposal of veterinary drug waste. The results indicated that 98.63% of horse owners administered antibiotics mainly to treat respira...
“There’s no blueprint for this kind of loss”: Resilience following the death of an equine in an equine-assisted services program.
Death studies    May 28, 2025   1-11 doi: 10.1080/07481187.2025.2510477
Kaufman SVA, Nieforth LO.For practitioners involved in equine-assisted services (EAS), the death of a horse can be a significant life disruption. This study aims to understand the communicative resilience processes that equine-assisted service practitioners express following the death of an equine in their program. This study analyzed secondary qualitative data from a cross-sectional online survey completed by 84 participants. Analysis revealed practitioners engaged in all five communicative resilience processes following the death of an equine in their program. This research extends current resilience research by exa...
Impact of hydrolysable and condensed tannin sources and levels on in vitro equine cecal fermentation.
Research in veterinary science    May 28, 2025   Volume 192 105732 doi: 10.1016/j.rvsc.2025.105732
Wottlin LR, Anderson RC, Bhatta R, Leatherwood J, Olson EG, Callaway TR, Ricke SC.Environmental impacts of animal agriculture are under intense scrutiny, thus strategies to decrease greenhouse gas emissions and volatile nitrogen waste are valuable. As dietary inclusion of tannins has been shown to decrease methanogenesis and improve nitrogen retention in ruminants, this study was designed to determine the effects of graded levels of hydrolysable tannin (chestnut) and condensed tannin (mimosa) on equine in vitro cecal fermentation. Methods: Two in vitro experiments were conducted, each with three replications per treatment. Treatments were 0.2 g alfalfa without or with 5, 1...
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