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Topic:Biomechanics

Biomechanics in horses refers to the study of the mechanical principles that govern movement and physical function in equine species. This field encompasses the analysis of gait, locomotion, and the forces exerted by and upon the horse's musculoskeletal system. Biomechanical studies often involve the use of motion capture technology, force plates, and computer modeling to assess how horses move and how various factors, such as conformation, training, and health status, influence their performance and soundness. Research in equine biomechanics contributes to understanding injury prevention, rehabilitation, and performance optimization. This page compiles peer-reviewed research studies and scholarly articles that explore the mechanics of movement, the impact of external and internal forces, and the applications of biomechanical analysis in equine care and management.
Proof of concept of a wearable IoT-based system for monitoring respiratory rate and surface temperature in horses.
Journal of equine veterinary science    November 8, 2025   Volume 155 105729 doi: 10.1016/j.jevs.2025.105729
Farias BJP, Furtado DA, Barbosa do Nascimento JW, Neto JPL, de Morais FTL, Santos TLS, Vasconcelos AS, Silva RC, Alves JIP, Mcmanus C, Silveira RMF....This study presents the development and proof of concept of a wearable monitoring system designed to measure respiratory rate (RR) and coat surface temperature (CST) in horses. The device integrates an embedded system based on an ESP-32 microcontroller equipped with sensors for RR (strain gauge) and CST (DS18B20) Data are transmitted in real time via Wi-Fi and stored locally on SD cards. The system was validated against conventional manual methods under field conditions. Agreement between methods was evaluated using Intraclass Correlation Coefficients (ICC) and Bland-Altman plots. Results show...
Quantitative assessment of gait alterations and variability in sulky-driven Standardbred trotters after a standardised exercise test.
Equine veterinary journal    November 6, 2025   doi: 10.1111/evj.70114
Aarts RM, Smit IH, Ferraz GC, Rhodin M, Serra Braganca FM, Hernlund E.Assessment of equine metabolic and locomotory responses to quantified levels of physical exercise is needed to support the creation of tailored protocols for optimal training adaptation. The locomotory response to exercise in horses is not fully understood. Objective: To investigate the effect of a standardised exercise test (SET) on upper-body and limb kinematics and stride-to-stride variability in Standardbred Warmblood trotters. Methods: Experimental repeated-measures field study. Methods: Thirteen Standardbred trotters, equipped with nine inertial measurement units (IMUs), performed an inc...
Reliability, agreement and variability of a markerless computer vision algorithm for equine gait analysis under field conditions.
Equine veterinary journal    November 4, 2025   doi: 10.1111/evj.70109
Key K, Berg K, Kirkegaard J, Andresen KR, Skov Hansen S.Computer vision-based algorithms offer accessible alternatives for equine gait analysis but require thorough assessment under diverse conditions. Objective: To evaluate a proprietary vision-based algorithm's reliability in measuring vertical displacement signals (VDS) at the eye, withers and croup, alongside groundline estimation, for horses trotting on straight lines and circles under field conditions. Methods: Cross-sectional comparative study evaluating agreement, variability and reliability of a markerless computer vision algorithm. Methods: We obtained 67 handheld iPhone recordings from 3...
Determinants of stride parameters in Thoroughbreds racing in Japan.
American journal of veterinary research    October 28, 2025   Volume 87, Issue 2 ajvr.25.07.0273 doi: 10.2460/ajvr.25.07.0273
Takahashi Y, Pfau T, Tsuruoka F, Yoshida T, Edwards WB, Mukai K.To identify the race- and horse-level factors affecting stride parameters during Thoroughbred races in Japan. Unassigned: Global Navigation Satellite System sensors were attached to 921 horses (1,189 starts) participating in 83 races, with distances ranging from 1,000 to 1,800 m, held from April through July 2024. Stride frequency and stride length were calculated from speed spectrograms at 3 racing phases (phase 1, 200 m after gate open; phase 2, 10 m after reaching the final straight stretch; and phase 3, 130 m before the finishing line). Additionally, 10 variables (race distance, surface ty...
Ultrasonography and shear wave elastography of equine skin: a preliminary study.
American journal of veterinary research    October 16, 2025   Volume 87, Issue 1 ajvr.25.05.0186 doi: 10.2460/ajvr.25.05.0186
Braga C, Prazeres J, Borges J, Ribeiro G.To noninvasively characterize skin thickness and elasticity in healthy horses using B-mode ultrasound and shear wave elastography (SWE) and to verify whether there are differences between the skin of stabled and outdoor horses. Unassigned: 10 clinically healthy horses were enrolled in this study. Five animals were housed exclusively indoors (indoor group), and 5 had temporary or permanent access to an outdoor environment (outdoor group). All horses underwent bilateral ultrasonographic examination of 3 anatomical regions: neck, withers, and girth region caudal to the elbow. Skin thickness was a...
Dynamic Mobilization Exercises Improve Activity and Stride Parameters Measured with Accelerometry in Sedentary Horses.
Animals : an open access journal from MDPI    October 10, 2025   Volume 15, Issue 20 2943 doi: 10.3390/ani15202943
Saitua A, Pérez-Umbría J, García-Álamo K, Muñoz A.Dynamic mobilization exercises (DME) are an effective strategy to prevent musculoskeletal injuries and promote back health in sport horses. Previous studies focused mainly on multifidus muscle cross-sectional area, with limited data on locomotion and adaptation timing. This study evaluated locomotor changes using accelerometry, over 8 weeks of DME application in 14 sedentary horses: a DME group ( = 8) performing 10 different DME (3 neck flexions, 1 neck extension and 3 lateral bending exercises to each side), 5 repetitions of each DME per session, 3 sessions/week, and a control group ( = 6), t...
Mechanical characterization of the equine linea alba and finite element modeling of suture patterns effects on its closure.
Journal of the mechanical behavior of biomedical materials    October 10, 2025   Volume 173 107228 doi: 10.1016/j.jmbbm.2025.107228
Bollens J, Gheysen L, Verkade M, Stael J, Martens A, Segers P.Postoperative incisional complications are common in horses following abdominal surgery, which typically involves an incision through the abdominal wall along the linea alba. The linea alba is a fibrous band running in the craniocaudal direction along the ventral abdomen. This incision is closed with sutures, where the choice of suture pattern and surgical technique has shown to influence the rate of complications. Therefore, this study investigated how different suture patterns and variations influence the stresses in the tissue by combining experimental and computational biomechanics. The me...
A mathematical model of metacarpal subchondral bone adaptation, microdamage and repair in racehorses.
Journal of the Royal Society, Interface    October 1, 2025   Volume 22, Issue 231 20250297 doi: 10.1098/rsif.2025.0297
Pan M, Malekipour F, Pivonka P, Morrice-West AV, Flegg JA, Whitton RC, Hitchens PL.Fractures of the distal limb in Thoroughbred racehorses primarily occur because of accumulation of bone microdamage from high-intensity training. Mathematical models of subchondral bone adaptation of the third metacarpal lateral condyles are capable of approximating existing data for Thoroughbred racehorses in training or at rest. To improve upon previous models, we added a dynamic resorption rate and microdamage accumulation and repair processes. Our ordinary differential equation model simulates the coupled processes of bone adaptation and microdamage accumulation, and is calibrated to data ...
The effect of heel elevation on the stiffness gradient index of the digital flexor tendons in the equine forelimb of clinically normal horses.
Frontiers in veterinary science    October 1, 2025   Volume 12 1610788 doi: 10.3389/fvets.2025.1610788
Shaw KA, Brounts SH.To evaluate the effect of heel elevation on measurement of stiffness gradients by means of acoustoelastography in the digital flexor tendons of clinically normal horses. Unassigned: 15 clinically normal horses. Unassigned: For each horse, stiffness gradient index (SGI) for superficial digital flexor tendons (SDFT) and deep digital flexor tendons (DDFT) were evaluated in both forelimbs at 0, 4 and 8 degrees of heel elevation. Acoustoelastography (AEG) was used for data acquisition at three sites, approximately 6, 12, and 18 cm distal to the accessory carpal bone in the metacarpal region. Lif...
Ex vivo biomechanical testing of a three-dimensional printed titanium plate and spacer construct and 4.5 mm locking compression plate for ventral cervical fusion of C4-C5 in the horse.
Veterinary surgery : VS    September 29, 2025   Volume 54, Issue 7 1344-1352 doi: 10.1111/vsu.70014
Zedler S, Jukic C, van Eps A, Stefanovski D, Genton M, Rossignol F.To compare the biomechanical properties of a three-dimensional (3D) printed titanium plate and spacer (3DM) and a 4.5 mm broad locking compression plate (LCP) for intervertebral fusion of C4-C5 in the horse. Methods: Ex vivo experimental study. Methods: Twenty-four equine cadaveric cervical spines. Methods: Twenty-four cadaver equine cervical spines were randomly assigned to ventral intervertebral fusion with either 3DM (n = 12) or LCP (n = 12) at C4-C5. Four-point bending tests were performed in flexion (3DM n = 6, LCP n = 6) and extension (3DM n = 6, LCP n = 6) in a sin...
Agreement between subjective evaluations and a markerless AI-based gait analysis system during lungeing assessment in traditional racehorses.
Journal of equine veterinary science    September 28, 2025   Volume 154 105704 doi: 10.1016/j.jevs.2025.105704
Meistro F, Ralletti MV, Rinnovati R, Spadari A.Subjective lameness evaluation during lungeing is routinely performed in equine practice, but its consistency remains limited, especially in cases of mild or complex asymmetry. Objective: This study aimed to assess the agreement between subjective gait evaluations and a markerless AI-based gait analysis system (OAI-MS) in traditional racehorses during lungeing. Intra- and inter-observer agreement of subjective evaluations was also investigated. Methods: 24 traditional racehorses were assessed during routine pre-race inspections (T0) while trotting on a soft surface. Two experienced equine clin...
Comparing the Difference in Traction Between the Bare Hoof, Iron Horseshoes and Two Glue-On Models on Different Surfaces.
Sensors (Basel, Switzerland)    September 26, 2025   Volume 25, Issue 19 5975 doi: 10.3390/s25195975
Siedler C, Zinkanel YM, Schramel JP, Peham C.The interaction between equine hooves and various ground surfaces is a critical factor for injury prevention and performance in modern equestrian sports. Accurate measurement of surface grip is essential for evaluating the effectiveness of different hoof protection systems. This study introduces the Vienna Grip Tester (VGT), a novel sensor-based device developed to quantify rotational resistance-an important parameter for assessing hoof-surface interaction. The VGT utilizes a torque wrench and spring-loaded mechanism to simulate lateral hoof movements under a standardized vertical load (~700 N...
Thoroughbreds deemed to be most at risk by inertial measurement unit sensors suffered a fatal musculoskeletal injury at a higher rate than other racehorses.
Journal of the American Veterinary Medical Association    September 17, 2025   Volume 264, Issue 1 81-88 doi: 10.2460/javma.25.04.0268
Mc Sweeney D, Wang Y, Palmer SE, Holmströem M, Donohue KD, Farnsworth KD, Sanz MG, Lambert DH, Bayly WM.To determine whether screening of racing Thoroughbreds with accelerometer-based inertial measurement unit sensors and a specifically trained algorithm identified horses most at risk for fatal musculoskeletal injury (FMI) and whether age, gender, race distance, and track surface were associated with increased risk. Unassigned: Stride data from 28,481 races by 11,834 Thoroughbreds from July 25, 2021, until May 4, 2024, were assigned an algorithm-based risk score from 1 to 6 (6 = greatest risk). Logistic regression models examined the association between incidence of fatal injuries and risk score...
Temperatures of the Mouthpiece of the Bit of Carriage Horses over a Period of 11 Months.
Animals : an open access journal from MDPI    September 7, 2025   Volume 15, Issue 17 2623 doi: 10.3390/ani15172623
Krcal C, Licka T.Besides oral temperature, meteorological parameters are expected to be relevant for mouthpiece temperature, potentially influenced by the material, surface area and weight of the bit. This study measured the temperature of the mouthpieces at the corner of the mouth while they were in use on 58 carriage horses during the four 2024 seasons. Stainless steel, copper, and copper-steel bits were tested in three shapes: Butterfly Liverpool, Liverpool, and Loose Ring Snaffle with four rings. Additionally, surface temperatures of inner thighs, the ground, and buildings were measured using infrared ther...
2D-shear wave elastographic features of normal and injured equine superficial digital flexor tendons.
Equine veterinary journal    September 5, 2025   doi: 10.1111/evj.70091
Guerri G, Bandera L, Straticò P, Palozzo A, Di Nunzio L, Celani G, Varasano V, Vignoli M, Petrizzi L.Superficial digital flexor tendon (SDFT) injuries cause progressive loss of its mechanical properties. Two-dimensional shear wave elastography (2D-SWE) provides information about tissue stiffness. Objective: To determine the feasibility, repeatability, and reproducibility of 2D-SWE of healthy and pathological forelimb SDFT. Methods: Prospective observational cohort study. Methods: Horses were divided into a noninjured Group (H) and a Group with tendinopathy of the SDFT (P). 2D-SWE of both fore SDFTs was performed at three metacarpal levels (A, B, and C, from proximal to distal). Qualitative an...
The Effect of Girth Design and Girth Tension on Saddle-Horse Pressures and Forelimb Stride Kinematics in Rising Trot.
Animals : an open access journal from MDPI    August 29, 2025   Volume 15, Issue 17 2540 doi: 10.3390/ani15172540
Marlin D, Randell O, Mayhew E, Blake R.The aim of this study was to investigate the effect of girth design and girth tension; six horses regularly ridden were used. Each horse underwent four experimental sessions in an unbalanced Latin-square design with two girth tensions (8 kg or 16 kg) and two girth designs (straight girth (S) or anatomical girth (A)). Pressure between the saddle and the horse was measured at 100 Hz with a pressure mat (0.5 sensels per cm). Notably, 2D limb kinematics were determined from anatomical markers placed on the fore and hindlimbs. Video was collected at 240 fps. There was no significant effect of girth...
A longitudinal study of radiodensity and radiographic appearance of the proximal sesamoid bones in Thoroughbred racehorses.
Equine veterinary journal    August 21, 2025   doi: 10.1111/evj.70075
Boros K, Dyson S, Pollard D, Nagy A.There are limited data on long-term follow-up of radiographic changes of the proximal sesamoid bones (PSBs) in Thoroughbred racehorses. The effect of training and racing on radiodensity of the PSBs in live Thoroughbreds has not been described. Objective: To follow radiographic changes of the PSBs in racehorses over 2 years and describe alterations in overall radiodensity. Methods: Prospective cohort study. Methods: Forty yearlings were enrolled at the first examination (T0). Re-examinations were performed four times, approximately 6 months apart, on 31 (T1), 23 (T2), 13 (T3) and 8 (T4) horse...
Exploring Monthly Variation of Gait Asymmetry During In-Hand Trot in Thoroughbred Racehorses in Race Training.
Animals : an open access journal from MDPI    August 20, 2025   Volume 15, Issue 16 2449 doi: 10.3390/ani15162449
Pfau T, Forbes B, Sepulveda-Caviedes F, Chan Z, Weller R.Based on fundamental mechanics, movement and force associate head and pelvic movement asymmetry with asymmetry of force production. We investigate, how often racehorses undergoing strenuous training regimens show evidence of switching between "preferred" limbs, i.e. one limb producing increased force, when assessed at monthly intervals? We hypothesize that clinical asymmetry thresholds designed for "detecting lameness" are frequently exceeded and that when applying previously established Thoroughbred-specific repeatability values, horses rarely switch between showing left- and right-sided asym...
Does Muscle Development of Sport Horses Using Water Treadmill Exercise as Part of a Long-Term Training Programme Differ from That of Horses Not Using Water Treadmill Exercise?
Animals : an open access journal from MDPI    August 19, 2025   Volume 15, Issue 16 2426 doi: 10.3390/ani15162426
Tranquille C, Nankervis K, Tacey J, Hopkins E, Deckers I, Walker V, MacKechnie-Guire R, Newton R, Murray R.Water treadmill (WT) exercise has become a popular tool for equine training and rehabilitation. However, few studies describe the long-term effects of WT exercise in low water on muscle development (MD). This study's objectives were to compare MD changes over a 40-week period in sport horses that regularly used WT in low water within training (Group WTH, = 55) and a control group that did not (control, = 28). Subjective MD assessments were undertaken using an adaptation of a previously published method at weeks 0, 20 and 40. For Group WTH, MD significantly increased in the neck, pelvis and h...
Comparison of Gait Characteristics for Horses Without Shoes, with Steel Shoes, and with Aluminum Shoes.
Animals : an open access journal from MDPI    August 13, 2025   Volume 15, Issue 16 2376 doi: 10.3390/ani15162376
Gottleib K, Trager-Burns L, Santonastaso A, Bogers S, Werre S, Burns T, Byron C.Differences in horseshoe materials may have effects on gait that could change perceived esthetic qualities. Objective information regarding effects of shoeing on gait characteristics of horses is scant. The aim of this study was to determine differences in gait characteristics for horses under various experimental shoeing conditions (barefoot, aluminum shoes, steel shoes) on two surfaces (asphalt and soft footing) using body- and hoof-mounted sensors. We hypothesized that shoeing would affect hoof arc height during early (arc height a) and late (arc height b) swing phases but would not affect ...
A fitness tracker can be used interchangeably with a reference method for underwater single-lead electrocardiography but not heart rate variability analysis in swimming horses.
American journal of veterinary research    August 12, 2025   1-8 doi: 10.2460/ajvr.25.04.0113
Kisilevich Q, O'Connor SGP, Bayly WM, Léguillette R.To validate a single-lead ECG fitness tracker device (FT) against the reference multilead ECG recording device (RM) for heart rate (HR) and HR variability (HRV) monitoring of horses during routine swimming. Unassigned: 40 race-fit Thoroughbred racehorses were used for 5 days in May 2024. Surface ECG recordings were obtained simultaneously from the FT and RM devices in horses swimming 63.65 m across a pool. Electrocardiograms were reviewed and artifacts corrected when necessary, and correlations were analyzed between the 2 devices for HR and HRV parameters. Descriptive statistics and Bland-Altm...
Biomechanical testing of three constructs for prosthetic laryngoplasty in horses demonstrates advantages of differing metallic implants in the arytenoid cartilage.
Veterinary surgery : VS    August 10, 2025   doi: 10.1111/vsu.14328
Ysebaert MP, Johnson J, Marie U, Campos A, Verchrerin A, Ducharme NG, Rossignol F, Luedke LK.To compare the biomechanical stability of a novel arytenoid implant (tie-bolt, TB) with a suture anchor (SA) and standard laryngoplasty using suture alone (control) in equine larynges. Methods: Randomized ex vivo controlled experimental study. Methods: Thirty equine cadaver larynges. Methods: Larynges were randomly assigned to TB, SA, or control groups (n = 10 each). The force of abduction was measured for each construct, then constructs underwent cyclic loading (5-50 N for 3000 cycles) to assess elongation, followed by load-to-failure testing to assess ultimate load, displacement, stiffn...
Variation in Four Horse Gait Categories Illustrated by Quantitative Analysis With ANALOC-E.
Veterinary medicine international    August 6, 2025   Volume 2025 4906015 doi: 10.1155/vmi/4906015
Staiger EA, Pereira de Toledo A, Rizzato Paschoal V, Patterson Rosa L.Horse gaits are a trait highly selected and prized in diverse breeds. Meanwhile, gait classification relies mostly on subjective visual observations by evaluators. Noninvasive equipment able to track locomotion in horses and output quantitative gait parameters is not only helpful in evaluating locomotion but also in designating gait types and its variations. Equine locomotion pattern designation based solely on observer evaluation can be subjective; therefore, utilizing tools that provide quantitative results and track individual limb movements, especially during intermediate-speed gaits, can ...
A Markerless Approach for Full-Body Biomechanics of Horses.
Animals : an open access journal from MDPI    August 5, 2025   Volume 15, Issue 15 2281 doi: 10.3390/ani15152281
Shaffer SK, Medjaouri O, Swenson B, Eliason T, Nicolella DP.The ability to quantify equine kinematics is essential for clinical evaluation, research, and performance feedback. However, current methods are challenging to implement. This study presents a motion capture methodology for horses, where three-dimensional, full-body kinematics are calculated without instrumentation on the animal, offering a more scalable and labor-efficient approach when compared with traditional techniques. Kinematic trajectories are calculated from multi-camera video data. First, a neural network identifies skeletal landmarks (markers) in each camera view and the 3D location...
Quantification of the Effect of Saddle Fitting on Rider-Horse Biomechanics Using Inertial Measurement Units.
Sensors (Basel, Switzerland)    July 30, 2025   Volume 25, Issue 15 4712 doi: 10.3390/s25154712
Becard B, Sapone M, Martin P, Hanne-Poujade S, Babu A, Hébert C, Joly P, Bertucci W, Houel N.The saddle's adaptability to the rider-horse pair's biomechanics is essential for equestrian comfort and performance. However, approaches to dynamic evaluation of saddle fitting are still limited in equestrian conditions. The purpose of this study is to propose a method of quantifying saddle adaptation to the rider-horse pair in motion. Eight rider-horse pairs were tested using four similar saddles with small modifications (seat depth, flap width, and front panel thickness). Seven inertial sensors were attached to the riders and horses to measure the active range of motion of the horses' forel...
Histological Study of the Normal Proximal Third Interosseus Muscle Enthesis in the Equine Pelvic Limb.
Anatomia, histologia, embryologia    July 29, 2025   Volume 54, Issue 4 e70059 doi: 10.1111/ahe.70059
Joostens Z, Audigié F, de la Rebière de Pouyade G, Garigliany MM, Busoni V.In human medicine, the enthesis, or tendon-to-bone insertion, is generally considered a linked entity or 'enthesis organ' and plays a crucial biomechanical role. This study aimed to histologically assess the equine proximal third interosseus muscle (suspensory ligament) enthesis in 10 pelvic limbs from 7 horses. The area of the proximal third interosseus muscle enthesis was divided into 6 compartments based on post-mortem computed tomography and prepared for histology using haematoxylin/eosin and trichrome staining. The proximal third interosseus muscle enthesis showed a fibrocartilaginous nat...
Tendon/Ligaments: Structure and Composition.
The Veterinary clinics of North America. Equine practice    July 25, 2025   Volume 41, Issue 2 227-238 doi: 10.1016/j.cveq.2025.05.004
Durgam S.The mid-metacarpal SDFT, intrasynovial DDFT and suspensory ligament are frequently injured soft tissues in the equine athlete. The mid-metacarpal SDFT exhibits a hierarchical structure and encounters repetitive strains at its functional limit. The intrasynovial DDFT possesses fibrocartilage where opposing bony surfaces to facilitate resistance-free gliding and contains chondrocyte-like cells embedded in a proteoglycan-rich ECM. While suspensory ligament enthesis injuries are common, collagen and non-collagen ECM structure-function specializations at the bone-ligament interface are understudied...
Turf track surface interaction with speed and musculoskeletal injury risk in Thoroughbred racehorses.
Equine veterinary journal    July 24, 2025   doi: 10.1111/evj.70003
Legg KA, Gibson MJ, Gee EK, Rogers CW.Injury modelling based on changes in speed and stride characteristics of racehorses has become a primary industry focus for the Thoroughbred racing industry. However, speed and stride characteristics are strongly associated with track condition; therefore, reliable quantification of surface variables for use in future models is imperative. Objective: This study aimed to understand the interaction of objective turf track condition score (TCS) measurement with racing speed and injury in flat racing Thoroughbred horses. Methods: Retrospective time series analysis. Methods: Race-day data from 16 f...
In Silico Analysis of Stress Distribution in Proximal Interphalangeal Joint Arthrodesis in Horses Using a Locking Compression Plate.
Veterinary and comparative orthopaedics and traumatology : V.C.O.T    July 24, 2025   doi: 10.1055/a-2655-0004
de Souza AF, Pereira CAM, Gialain IO, Meira JBC, Ballester RY, De Zoppa ALDV.This study aimed to compare the stress distribution in proximal interphalangeal joint arthrodesis with locking compression plate in horses based on the type and method of screw implantation in the proximal hole and partial or total removal of implants after fusion.Finite element analysis.Proximal interphalangeal joint arthrodesis was simulated using a 3-hole, 4.5-mm narrow locking compression plate combined with two 5.5-mm transarticular screws. Models were created according to the type (cortex or locking) and method of implantation (uni- or bicortical) of the screw in the proximal hole of the...
Cryopreserved equine umbilical cord tissue allograft characterization and biocompatibility in vivo in musculoskeletal tissues: a controlled study.
BMC medicine    July 23, 2025   Volume 23, Issue 1 439 doi: 10.1186/s12916-025-04231-7
Bertone AL, Reinemeyer C, Tsaprailis G, Ragland D, Leise B.The use of micro-particulate allografts is rising, but knowledge about the protein characterization and biocompatibility of umbilical cord-derived allografts (UC) in vivo is limited. Methods: Proteomic analyses using mass spectrometry (MS) determined equine UC protein relative quantification and functions using total spectral counts (TSC). UC cytokines were quantified by enzyme-linked immunosorbent assay (ELISA). Three in vivo studies assessed recipient clinical and tissue biocompatibility in joints and ligaments. Results: Proteomics revealed 2645 annotated TSCs. Proteins of > 89 TSC we...