Equids are members of the family Equidae, which includes modern horses, donkeys, and zebras, as well as extinct species. Equids are characterized by their long limbs, single-toed hooves, and herbivorous diet, which is primarily composed of grasses. Horses, specifically, have been domesticated for thousands of years and have played significant roles in agriculture, transportation, and sport. This page compiles peer-reviewed research studies and scholarly articles that explore various aspects of equids and horses, including their evolution, physiology, behavior, and interactions with humans. The collected works provide insights into the genetic diversity, adaptive traits, and conservation efforts related to equid species.
Guyo M, Tareke M, Tonamo A, Bediye D, Defar G.Morphometric traits discrepancies are associated with production, reproduction, adaptation and behaviours in horses. It also determines the design of harnessing implements, physical injury level and working performance of the native horses. Thus, the study was conducted to evaluate morphometric traits and body conformation indices of horse ecotypes reared across four districts of Bale Zone, Ethiopia. Methods: Data of croup height, croup length, hip height, mid-back height, wither height, hip width, thoracic depth, chest width, body length, sternum height, thorax perimeter and cannon perimeter ...
Farmer Long J, Duberstein J, Costin K, Callaway T, Abrams A, Wassel B, Toal K, Duberstein K.Pasture tracks are a modern equine housing trend often implemented as a weight control strategy due to the belief that they promote movement, though this is not proven experimentally. To test movement of horses housed in pasture tracks as compared to conventional pasture housing, two experiments were performed using a track (0.2ha, 3.5m in width, 561 m total distance) created around the perimeter of a 2.0 ha pasture. In experiment 1, eight horses were paired and rotated between three treatment locations: track (T), small 2 ha pasture housed within the track (SP), and a larger 20 ha pasture on ...
Phetkarl T, Fungwithaya P, Lewchalermvong K, Sontigun N.The global prevalence of gastrointestinal (GI) and blood parasite infections in horses is a significant concern due to their substantial impact on morbidity, mortality, and economic losses in the horse industry. In Thailand, limited research has been conducted on these parasites in horse populations, and data from southern Thailand are lacking. Consequently, this study aimed to estimate the prevalence of GI and blood parasites in horses in Nakhon Si Thammarat province, Thailand. Unassigned: In total, 79 fecal and blood samples were collected from horses across 11 farms in Nakhon Si Thammarat p...
Yuen NKY, Harrison JJ, Wang ASW, McMahon IE, Habarugira G, Coyle MP, Bielefeldt-Ohmann H.An incursion and outbreak of Japanese encephalitis virus (JEV) was reported in Australia in 2021 and 2022, respectively. There was speculation that JEV may have been circulating in Australia unknowingly prior to the detection. In this study, we determined sero-prevalence and transmission of West Nile virus (WNV), Murray Valley encephalitis virus (MVEV) and JEV, prior to and post JEV incursion in a sentinel equine population in south-east Queensland (SEQ), Australia, using blocking ELISAs (screening test) and virus neutralisation test (confirmatory). Serum samples collected between 2018 and 202...
Karam B, Arndt S, Magdesian KG, Cullen T, Dechant JE.A newborn (5 h old) quarter horse colt was presented because of lethargy and severe abdominal distention. Uroperitoneum was suspected during initial workup, based on sonographic imaging and peritoneal fluid analysis. Definitive diagnosis was confirmed during exploratory celiotomy. Surgery revealed a congenitally abnormal allantoic stalk/urachal remnant and a failure of embryological fusion of the dorsal bladder wall. Recovery was successful and the animal is now a healthy, 4-year-old western performance gelding. These specific congenital abnormalities have not been previously documented in the...
Ortiz-Rodriguez JM, Bucci D, Tovar-Pascual L, Granata S, Spinaci M, Nesci S.Sperm metabolism consists of a sophisticated network of biochemical reactions and varies between species, resulting in different metabolic strategies for ATP production to maintain sperm functionality. ATP can be produced through glycolysis or in the mitochondria by oxidative phosphorylation (OXPHOS). Since OXPHOS is the predominant metabolic pathway in horses spermatozoa, various assessments of mitochondrial activity are used to evaluate fertility, utilizing techniques such as fluorescent probes analysed via microscopy or flow cytometry, and polarographic electrode assays to measure current f...
Broman A, Rawlinson JE, Bass L, Boscan P, Rao S.The use of proper sedative and regional anesthetic protocols is essential when performing equine dental surgical procedures under standing sedation. The efficacy of the rostral inferior alveolar nerve block via the mental foramen has not been previously studied. Aims of this study included determining the efficacy of the block, investigating whether any region (labial mucosa, alveolar mucosa, or teeth) was more reliably anesthetized, and if differences in efficacy existed between bilateral and unilateral blocks. In this blinded trial, 10 horses each were randomly assigned to receive a left uni...
Martin-Pelaez S, Fuente A, Takahashi K, Perez IT, Orozco J, Okada CTC, Neto CR, Meyers S, Dini P.Intracytoplasmic sperm injection (ICSI) is the current clinical practice for the in vitro production of equine embryos. The use of conventional fertilization methods such as in vitro fertilization (IVF), has historically been associated with poor success in horses. However, recent improvements have led to better outcomes with IVF, though only when using fresh semen, which limits its use in clinical practice. IVF remains in its infancy in equine reproduction, and several unknowns remain about the technique. One significant gap in knowledge concerns the morphokinetics of IVF embryos and how they...
Merridale-Punter MS, Wodajo AL, Elias B, Bakos AM, Zewdu H, Tesfaye R, Hailegebreal G, Sori T, El-Hage CM, Wiethoelter AK, Hitchens PL.Working equids support the livelihoods of millions of low-income households worldwide and face several welfare challenges. Although equipment-related wounds are common, little is known about specific risk factors. This cross-sectional study surveyed equids used for cart-work in three Ethiopian towns. Number, size, severity and location of wounds were recorded for each animal, as well as work equipment characteristics and indicators of equipment fit and assembly. Questionnaires were conducted with each cart-driver focusing on equipment practices and attitudes. Logistic regression models were ge...
Luan Y, Zhong L, Li C, Yue X, Ye M, Wang J, Zhu Y, Wang Q.Polydactyly was recorded before 100 BCE and attracted widespread interest because of its relationship to limb health and ancestral traits in horses. However, the underlying reasons for the development of polydactyly remain unclear. To search for polydactyly-related genes, we utilize a paternal half-sib family and screen for variants that match the mode of inheritance. Through this screening process, 77 variants in 65 genes are filtered. A missense variant (EqCab3.0 chr4: <107353368> A > G) (rs1138485164) in the 3rd exon of LMBR1 is identified as a source of amino acid sequence variatio...
Thielebein J, Bartling B, Hönicke C, Schmicke M.Semi-feral, free-roaming Konik polski horses are used in some European countries for preserving semi-open pasture landscapes. The estimation of their health status is still limited by insufficient data on various blood parameters. Therefore, our study aimed at the sex- and age-dependent analysis of haemogram and selected biochemistry parameters in healthy, semi-feral Koniks. In order to reach this aim, we took blood samples from 53 female and 18 male (8 uncastrated, 10 castrated) Koniks living in two Middle German nature reserves. They were of different age (9-266 months) and without signs of ...
Varner KM, Curtiss AL, Hogan PM, Love K, Dodam JR.In anaesthetised horses, bradycardia secondary to high vagal tone can reduce cardiac output and blood pressure. The use of anticholinergics in horses is limited due to concerns about ileus and abdominal discomfort. This retrospective study sought to determine the prevalence of post-operative abdominal discomfort in healthy horses that received atropine under isoflurane anaesthesia. Methods: A retrospective evaluation of 222 general anaesthesia events between January 2019 and December 2019 was undertaken. Methods: One hundred and eleven horses that received atropine were identified, and 111 cas...
Silva RC, Coelho GJ, Sousa MBF, Caldas CS, Maciel RP, Mezzomo R, Gomes DÍ, Neta ERDS, Tavares FB, Alves KS, Oliveira LRS.In this study, we analyzed the effects of including açaí kernel, in the experimental diets fed to horses on the intake and digestibility of their nutrient and the resultant effects on the behavior of horses. The experiments were conducted in Federal Rural University of Amazonia. Five mares with no defined breeds, were allocated to five treatments (the replacement of Mombaça grass (Megathyrsus maximus) with crushed açaí kernel at 0, 18.75, 37.50, 56.25, and 75% levels of dry matter), in a 5 × 5 Latin square design. The animals were housed in covered stalls with concrete floors, equipp...
Süß F, Guth S, Müller-Ehrenberg H, Röcken M, Staszyk C.The masticatory muscles of the horse are arranged in an asymmetrical pattern. Four individual muscles on the medial side of the mandible are opposed by one muscle (M. masseter) on the lateral side. However, recent studies on various herbivorous mammals indicate that the masseter muscle features a complex stratigraphic structure that might account for a functional diversity resembling an arrangement of several individual muscles. The functional consideration of the multidirectional equine masticatory movements leads to a similar hypothesis. In order to elucidate the detailed anatomy of the equi...
Tasnim Y, Stanley C, Rahman MK, Awosile B.We aim to detect beta-lactamase-producing Citrobacter sedlakii from horses and compare the genomic characteristics with isolates from humans. Results: We characterized phenotypically and genotypically nine C. sedlakii isolates from the feces of horses and then compared them to human-derived isolates using whole genome sequencing and phylogenomic methods. Seven isolates (7/9) were ampicillin-resistant, while at least one isolate was resistant to ceftriaxone, gentamicin, meropenem, and streptomycin. All nine isolates were carriers of the chromosomal-mediated blaSED-1 beta-lactamase gene, which...
Hernández-Avilés C, Ramírez-Agámez L, Varner DD, Raudsepp T, Love CC.Cases of stallion subfertility due to acrosome dysfunction have been recognized since the 1990s. While some of these were observed in stallions with reduced sperm motility and morphology, a more severe form has been reported in stallions with normal-to-excellent sperm quality parameters, which is also uniquely observed in individuals of the Thoroughbred registry. These stallions carry a susceptibility genotype (A/A-A A in the gene FKBP6, exon 5) for Impaired Acrosomal Exocytosis (IAE). Current clinical observations from our group have identified a few highly subfertile stallions from other bre...
Božić Jovanović V, Trailović R, Vićić I, Grković N, Radaković M, Karabasil N, Kaić A, Čobanović N.The aim of this study was to determine the effects of loading density and gender on blood welfare indicators, carcass bruises and horsemeat quality. Data were collected from twelve transports of 89 slaughter horses originating from the same collection centre. The transportation of slaughter horses at high loading densities (>200 kg/m), especially stallions, resulted in increased levels of lactate ( = 0.021), glucose ( < 0.0001), ceruloplasmin ( < 0.0001) and AOPP ( < 0.0001), but lower GSH levels ( 200 kg/m) during transport had lower levels of the aforementioned blood metabolites....
Hobbs SJ, Tatlisulu A, Johnson A, Rowlands SD, Lucey M, Martin JH, Graydon RW, Northrop AJ.The maximum galloping speeds of racehorses during a race are influenced by the functional performance of the ground ('going') amongst other factors. For turf racecourses in Britain, the ground is descriptively classified and numerically quantified on the morning of a race meeting by the clerk of the course and subsequently published to assist decision making. Importantly, this includes deciding whether a horse should or should not run. The going is also assessed and classified during the meeting by racing analysts using the normalized winning times from each race result. Differences between go...
Barrera G, Albiach-Serrano A, Guillén-Salazar F.Studies have shown that horses are sensitive to human attentional cues. Also, there is some evidence that they might be able to infer the knowledge state of a human and communicate intentionally with us. However, this ability is not fully characterized and certain behaviors, like gaze, asymmetric ears position or vocalizations, which could work as attention-getting behaviors, have been scarcely studied in this context. The aim of the present study was to assess whether horses' gaze toward a person, asymmetric ears position and vocalizations are subject to audience effects and adjust to human a...
Hall C, Kay R.Quality of life is dependent upon the extent to which behavioural needs are met, and the balance between pleasant and unpleasant lifetime experiences. In Part II of this systematic review, articles (n = 109) relating to horse-human interactions were reviewed to identify behavioural evidence of their positive or negative impact on the horse. The number of articles (n = 22) relating to the recognition of pain in horses, indicated the importance of identifying health issues, which are also likely to increase the aversiveness of interactions. These and articles relating to emotional reactivity tes...
Arias-Esquivel AM, Moscoso E, Umaña D, Arguedas M, Solano D, Durán G, Gómez A, Gutiérrez JM, León G.The immunization and industrial bleeding of horses are essential stages for producing snake antivenoms. In Costa Rica, the traditional method involves stimulating the antibody response of horses by periodically injecting venoms, collecting hyperimmune plasma over three consecutive bleeding days, and repeating this process every eight weeks. While this method does not cause major physical or hematological issues in horses, the associated stress has not been evaluated. We compared this traditional method with an alternative method that involves injecting venoms, collecting hyperimmune plasma in ...
Costa Kamura BD, Albertino LG, Cavallari RR, Queiroz de Souza L, Rodrigues LT, Barros de Sousa Pereira I, Rocha NS, Takahira RK, Briscola Pereira WA....Africanized crossbred bees (Apis mellifera) originated in Brazil in 1957, and since then, the number of accidents involving humans and animals has significantly increased. Although they are considered clinical emergencies, there are few reports describing the clinical and pathological aspects of bee envenomation in horses. In this context, this report aims to describe the clinical and pathological features of bee toxicity after massive bee envenomation in three horses. The horses were referred to the veterinary hospital the day following the attack, and after clinical and laboratory examinatio...
Seck M, Carder G, Wathan J, Randau M, Fletcher K, Proops L.The role that working equids play in both rural and urban communities in low and middle-income countries is invaluable. They contribute to daily tasks such as carrying food, water and people, support income generation, and are of social and cultural importance. Despite their importance, global standards of working equid welfare are low. Many variables can impact the welfare status of animals under human care, but often specific factors are explored in isolation. Factors can include, but are not limited to an owner's socioeconomic status and their attitudes and beliefs towards animals. In this ...
Mustefa A, Engdawork A, Sinke S.Ethiopian horses are multipurpose horses that have socioeconomic significance for smallholder farmers. However, studies regarding their husbandry practices have received little attention. Thus, the current study was conducted to assess the horse husbandry practices in the southwest Ethiopia. Data were collected through semi-structured questionnaires from a total of 196 randomly selected respondents. The general linear model and frequency procedures of the Statistical Analysis System (SAS 9.0) were used to analyze the quantitative and qualitative data, while indices were calculated using Micros...
Blanco-Doval A, Barron LJR, Bustamante MÁ, Aldai N.Mare milk has often been considered a food product with potential functional properties. However, the bioactive compound composition of mare milk, including vitamins and other minor bioactive compounds, as well as factors affecting this composition have scarcely been studied. Therefore, the present study aimed to characterize the changes during lactation in the content of water- and fat-soluble vitamins and total polyphenols, and the total antioxidant capacity of mare milk from semi-extensive farms. A total of 310 individual milk samples from 18 mares belonging to three commercial farms and 12...
Asti V, Ablondi M, Molle A, Zanotti A, Vasini M, Sabbioni A.The shift of the horse breeding sector from agricultural to leisure and sports purposes led to a decrease in local breeds' population size due to the loss of their original breeding purposes. Most of the Italian breeds must adapt to modern market demands, and gait traits are suitable phenotypes to help this process. Inertial measurement unit (IMU) technology can be used to objectively assess them. This work aims to investigate on IMU recorded data (i) the influence of environmental factors and biometric measurements, (ii) their repeatability, (iii) the correlation with judge evaluations, and (...
Huangsaksri O, Wonghanchao T, Sanigavatee K, Poochipakorn C, Sukhong P, Chanda M.Lunging is a training technique commonly performed in a round pen or on a lunge line. This study investigated the effort intensity, autonomic responses, and method agreement in applying different lunging protocols to untrained ponies. Sixteen untrained ponies (aged 13.6 ± 6.3 years and weighing 358 ± 47.4 kg) were studied. Each pony underwent the same research-focused lunging program on a lunge line and, subsequently, in a round pen with a two-day interval between sessions. The heart rate variability (HRV) and effort intensity, indicated as a percentage of maximum heart rate (%HRmax), were d...
Tou K, Cawley A, Noble G, Loy J, Bishop D, Keledjian J, Sornalingam K, Richards S, Fu S.Detecting the use of bisphosphonates (BPs) in equine athletes is of interest to regulators and laboratories due to the threat to welfare issues for the potential to provide analgesic effects and manipulating bone structure. The detection of BPs in biological matrices is challenging due to erratic biological elimination and inconsistent analytical recoveries. Therefore, complementary approaches are needed to provide evidence of their misuse in racehorses. BPs have two sub-classes: nitrogenous and non-nitrogenous. This study investigated plasma elimination following administration of one example...
Lyons ET, Tolliver SC, Stamper S, Drudge JH, Granstrom DE, Collins SS.Praziquantel injectable formulation was administered at 0.5 mg k-1 per os to 24 equids naturally infected with 1-183 (average 40) Anoplocephala perfoliata. Drug activity was evaluated by a modified critical test method with necropsy 24 h after treatment. There was variable efficacy of 0-100% (aggregate average 85%); for 18 equids, 93-100%, for three equids, 70-85%, and for three equids, 0-20%.
Cymbaluk NF, Bristol FM, Christensen DA.Plasma ceruloplasmin activities and plasma Cu and Zn concentrations were determined in 215 clinically normal equids of various ages and breeds. Newborn foals, regardless of breed, were hypocupremic, compared with adolescent and mature horses. The mean plasma Cu concentration of newborn Standardbred-Thoroughbred (STD-TB) foals was 2.9 mumol/L, which was about one-sixth of plasma Cu concentrations of mature horses. Newborn draft-cross foals had higher (4.6 mumol/L) plasma Cu concentrations than did newborn STD-TB foals, but plasma Cu content was only one-fifth of the dams' concentrations. Draft-...
Greene HM, Wickler SJ, Anderson TP, Cogger EA, Lewis CC, Wyle A.Arterial and venous blood were analysed at rest and post exercise for pH, PCO2, and PO2, and bicarbonate ([HCO3-]), base excess (BE), and strong ion difference (SID) were calculated in response to a 10 day sojourn to 3800 m. Pulmonary artery pressures (PAP) were measured at rest. Post exercise samples were restricted to venous blood. The equids (n = 6) experienced a profound hypoxia-hypocapnia and a respiratory alkalosis. PaO2 decreased 42% and PaCO2 41%. PaCO2 increased to 80% of initial values after 8 days at altitude. Arterial [HCO3-] decreased by 34%; however, it returned to normal by Day ...
Vollmerhaus B, Roos H, Knospe C.The enamel cups of equids originated phylogenetically through several phases in Oligocene and Miocene horses, which readjusted from hard to soft leaf food and from double nutrition (leaves and grass) to a pure grass intake. This has been proved experimentally. The resulting construction of the incisor is continually changed on its occlusal surface by erosion according to the pattern of the enamel crests. Referring to the whole incisor tooth, this results in an equid life cycle in which the good grip of the occlusal surface and the efficiency of ingestion favours the young, sexually mature hors...
Byambaragchaa M, Choi SH, Joo HE, Kim SG, Kim YJ, Park GE, Kang MH, Min KS.Equine chorionic gonadotropin (eCG), produced by the endometrial cups of the placenta after the first trimester, is a specific glycoprotein that displays dual luteinizing hormone (LH)-like and follicle-stimulating hormone (FSH)-like effects in non-equid species. However, in equidaes, eCG exhibits only LH-like activity. To identify the specific biological functions of glycosylated sites in eCG, we constructed the following site mutants of N- and O-linked glycosylation: eCGβ/αΔ56, substitution of α-subunit N-linked glycosylation site; eCGβ-D/α, deletion of the O-linked glycosylation sites ...
Ryder OA, Sparkes RS, Sparkes MC, Clegg JB.1. Through the use of isoelectric focusing and peptide analysis, the hemoglobins of Przewalski's horse. Equus przewalskii and the domestic horse, E. caballus have been compared. 2. Przewalski's horses have two separate alpha-globin chain polymorphisms similar to domestic horses. Each hemoglobin phenotype could be accurately determined by isoelectric focusing. 3. Confirmation of the electrofocusing hemoglobin determinations was made by comparison to amino acid composition analyses of purified tryptic peptides and by analysis of the rare hemoglobins phenotypes observed in a family of Norwegian t...
Young M, Allen WR, Deutz NE.Free amino-acid concentrations were measured in maternal venous and fetal umbilical vein plasma, and in the allantochorion, of Thoroughbred mares at term. Concentrations in maternal and fetal plasma were similar to those reported previously in equids. The concentrations of free amino-acids in the allantochorion were higher than those in the maternal and fetal plasmas and were characterised by high levels of the nonessential amino-acids as observed in other species. Fourteen of the 20 amino-acids measured had similar allantochorion/umbilical vein concentration ratios suggesting that simple grad...
McNeill LR, Bott RC, Mastellar SL, Djira G, Carroll HK.In South Dakota, the status of equid well being is relatively unknown. This study sought to (a) gain understanding about the current perceptions of nonhuman animal well being in South Dakota, with an emphasis on horses and other equids; (b) determine the level of care equids are reportedly receiving and the perceived challenges to equine well being in South Dakota, and (c) determine if people from diverse geographical locations (east or west of the Missouri River) have similar views on the well being of equids in South Dakota. Respondents indicated the current level of equid well being in Sout...
The increasing interest in the study of spontaneous (SY) and contagious yawning (CY) was so far focused on several taxa, especially primates. Here, we focused on SY and CY in horses, a suitable species due to their complex social dynamics that has been largely overlooked in research on these phenomena. By analyzing videos of 48 horses on pasture, we identified 2 yawning morphologies: Covered ( ) and Uncovered Teeth ( ). Using EquiFACS, we quantitatively demonstrated that and differ in terms of muscle recruitment. Moreover, we provide the first evidence for the presence of CY by comparing 2...
Sasaki M, Endo H, Yamagiwa D, Yamamoto M, Arishima K, Hayashi Y.The skeletal feature was observed in Przewalski's horse (Equus przewalskii) and the domestic horse (E. caballus). As results of the observations, remarkable differences were found in the scapula. The caudal border of the scapula in Przewalski's horse is curved in the caudal direction more than that of the domestic horse. Moreover, in the domestic horse, the sharp caudal border is formed by the shift of the outer muscular line (Linea muscularis) to the border. In Przewalski's horse, however, the caudal border is rounded because the outer muscular line does not shift to the border but lies besid...
Loftus L, Asher L, Leach M.Over the last twenty years the definition of good animal welfare has advanced from the 'absence of negative welfare states' to the aim of identifying the presence of positive welfare states; however, research on positive animal welfare is relatively new. Consequently, through expert consultation, this study aimed to synthesise knowledge regarding domesticated equine emotional state, specifically methods to induce and measure positive affective states, which could be of significant benefit to equine welfare across sectors. A Delphi consultation of experts in the field of equine behaviour, welfa...
Kowalczyk K, Frąckowiak H.Considerable consistency in the arterial pattern of the head has been observed in species of Artiodactyla, but few studies have examined the order Perissodactyla. Here, we describe arteries supplying the intermandibular, mental, masseteric, buccal, labial, and nasal regions in eight perissodactylans, including representing of all families comprising this order. Observations were made on a total of 45 preparations of head arteries, obtained by injection of arteries with acetone-dissolved stained vinyl superchloride or stained latex LBS3060. In the Equidae species alone it was found that the fac...
Scott TW, Olson JG, All BP, Gibbs EP.Sensitivity and specificity of an antigen-capture ELISA vs virus isolation in cell culture were evaluated for the detection of eastern equine encephalomyelitis (EEE) virus in the brain tissue of naturally infected equids. Brain specimens from 16 equids with neurologic disease were examined by ELISA and by inoculation onto baby hamster kidney cell cultures. Of 10 brain samples from which virus was isolated in the cell culture bioassay, all were correctly identified as containing EEE virus antigen by ELISA. None of the remaining 6 specimens, without detectable infectious EEE virus, contained det...
Arrighi S, Romanello MG, Domeneghini C.Following previous studies about the ultrastructure of male genital tract in parental species, a comparative study of epididymis of one of the possible hybrids, the mule, has been undertaken. Apart from small differences, general features of epididymal epithelium in the mule are similar to those of parental species. However, extension of our studies from the donkey to the horse to the hybrid permits a deeper insight into the morphology of this tract of excurrent duct. In the meantime, it is possible to evidence some features, sometimes shared with other species if taken separately, which in th...
Parashar R, Singla LD, Batra K, Kumar R, Kashyap N, Kaur P, Bal MS.To unravel equid trypanosomosis caused by Trypanosoma evansi in Punjab state of India, a cross sectional study was designed by utilizing parasitological and sero-molecular tools with objective to assess the prevalence of T. evansi in association with various risk factors in all agroclimatic zones of Punjab state of India. Parasitological Romanowksy stained thin blood smears (RSTBS) to detect patent infection, molecular techniques polymerase chain reaction I (PCR I; TBR 1/2 primers; targeting minichromosomal satellite DNA of T. evansi), polymerase chain reaction II (PCR II; TR 3/4 primers; targ...
Kydd J, Miller J, Antczak DF, Allen WR.The maternal immunological response to the developing equine fetus was investigated in 69 pregnancies of various genotypes. In normal intraspecies horse pregnancy (N = 16), 94% of mares produced strong cytotoxic antibody responses to paternal histocompatibility antigens which were first detectable between 44 and 70 days after ovulation. In all other types of pregnancy examined (intraspecies donkey, N = 19; interspecies mule, N = 6; interspecies hinny, N = 2; extraspecies horse-in-donkey, N = 3; and extraspecies donkey-in-horse, N = 21), antibody production was observed less frequently, and som...
Cohen L, Bousfield GR, Ben-Menahem D.The pituitary LHβ and placental CGβ subunits are products of different genes in primates. The major structural difference between the two subunits is in the carboxy-terminal region, where the short carboxyl sequence of hLHβ is replaced by a longer O-glycosylated carboxy-terminal peptide in hCGβ. In association with this structural deviation, there are marked differences in the secretion kinetics and polarized routing of the two subunits. In equids, however, the CGβ and LHβ subunits are products of the same gene expressed in the placenta and pituitary (LHβ), and both contain a carboxy-te...
Shotton JCR, Justice WSM, Salguero FJ, Stevens A, Bacci B. Pituitary pars intermedia dysfunction (PPID), also known as equine Cushing's disease, is widely reported in middle-aged to older domestic equids but to date reported in only one nondomestic equid, the onager ( Equus hemionus onager). This case series reports clinical, hematological, and pathological findings consistent with PPID in two further equid species: one Chapman's zebra ( Equus quagga chapmani) and five Przewalski's horses ( Equus ferus przewalskii). The case series reports basal adrenocorticotropic hormone (ACTH) testing as a method to diagnose and monitor PPID in zoological equid...
Sisken BF, Zwick M, Hyde JF, Cottrill CM.This review covers the development and maturation of the cerebellum of the horse and compares this developmental sequence with that of man, mouse and chicken. These comparisons attempt to correlate morphological and neurochemical features, developmental time and functional performance necessary for survival at birth. Although there is great disparity between these 3 species, the basic anatomical structures of the cerebellum are present as are specific cellular groups, synapses and neurochemical markers. In addition to this structural homogeneity, other attributes of the cerebellum are its easi...
Antczak DF, Allen WR.There is little evidence for maternal immunological recognition of pregnancy in most species with the striking exception of the members of the genus Equus. Almost all mares make strong cytotoxic antibody responses to paternally inherited fetal antigens by Day 60 of gestation. Most of these responses are directed against antigens of the Major Histocompatibility Complex (MHC), which constitutes the primary immunogenetic barrier to successful organ transplantation. The source of fetal MHC antigens in the pregnant mare appears to be the specialized trophoblast cells of the chorionic girdle region ...