Exercise physiology in horses involves the study of the physiological responses and adaptations of horses to physical activity. This field examines how exercise impacts various systems in the equine body, including the cardiovascular, respiratory, muscular, and metabolic systems. Researchers focus on understanding how these systems interact during different types and intensities of exercise, and how they contribute to performance and recovery. Key areas of interest include the assessment of aerobic and anaerobic capacity, muscle fiber composition, energy metabolism, and thermoregulation. This page compiles peer-reviewed research studies and scholarly articles that explore the mechanisms of exercise physiology in horses, with an emphasis on performance optimization, training regimens, and the prevention of exercise-related disorders.
Brighton C, Olsen E, Pfau T.Standalone 'low-cost' inertial measurement units (IMUs) could facilitate large-scale studies into establishing minimal important differences (MID) for orthopaedic deficits (lameness) in horses. We investigated accuracy and limits of agreement (LoA) after correction of magnitude-dependent differences of a standalone 6 degree-of-freedom IMU compared with an established IMU-based gait analysis system (MTx) in six horses for two anatomical landmarks (sacrum and sternum). Established symmetry measures were calculated from vertical displacement: symmetry index (SI), difference between minima (MinDif...
Viry S, Sleimen-Malkoun R, Temprado JJ, Frances JP, Berton E, Laurent M, Nicol C.In riding, most biomechanical studies have focused on the description of the horse locomotion in unridden condition. In this study, we draw the prospect of how the basic principles established in inter-personal coordination by the theory of Coordination Dynamics may provide a conceptual and methodological framework for understanding the horse-rider coupling. The recent development of mobile technologies allows combined horse and rider recordings during long lasting natural events such as endurance races. Six international horse-rider dyads were thus recorded during a 120 km race by using two t...
Assenza A, Tosto F, Casella S, Fazio F, Giannetto C, Piccione G.Prothrombin Time (PT), Activated Partial Prothrombin Time (APTT), Fibrinogen concentration (Fbg) and Platelet number (Plt) were evaluated in 20 young athletic horses during a training program. A standardized exercise test (SET) was performed every month for three months. The V4 variations (the speed, in m/min, reached at the blood lactate concentration of 4 mmol/l) obtained for each test were calculated to assess the effect of training program on athletic performance. Blood samples were collected at 20-day intervals over a period of 80 days from the beginning of the training program. The V4 (P...
Walker VA, Dyson SJ, Murray RC.Development of core muscle strength is important for training and during rehabilitation following injury. A Pessoa training aid (PTA) is a system of ropes and pulleys which is commonly used during equine training and rehabilitation, but there is limited information on its effectiveness. The objective of the study was to determine the effect of the PTA on the temporal, linear and angular kinematics of the working trot. Influence of testing order on effect of a PTA was assessed in four horses. Twelve riding horses were lunged at working trot on a 16 m diameter circle without (WO) and with a PTA ...
Miranda MC, Queiroz-Neto A, Silva-Júnior JR, Pereira MC, Soares OA, Borghi RT, Ferraz GC.To compare five different protocols for estimating the lactate minimum speed (LMS) with that for estimating the maximal lactate steady state (MLSS) in Arabian horses, in order to obtain a more rapid method for monitoring aerobic capacity and prescribing training schedules. Methods: Eight purebred Arabian horses were conditioned to exercise on a treadmill for 12 days then submitted to three to five exercise sessions to determine the MLSS. Blood samples were collected from a jugular catheter at specific intervals for measurement of lactate concentrations. The MLSS was the velocity maintained dur...
The Journal of physiologyJuly 1, 2013
Volume 591, Issue 18 4499-4513 doi: 10.1113/jphysiol.2013.257956
Vengust M, Staempfli H, Viel L, Swenson ER, Heigenhauser G. During intense exercise in horses the transvascular fluid flux in the pulmonary circulation (Jv-a) represents 4% of cardiac output (Q). This fluid flux has been attributed to an increase in pulmonary transmural hydrostatic forces, increases in perfused microvascular surface area, and reversible alterations in capillary permeability under conditions of high flow and pressure. Erythrocyte fluid efflux, however, accounts for a significant fraction of Jv-a. In the lung the Jacobs-Stewart cycle occurs with diffusion of CO2 into alveolar space with possible accompanying chloride (Cl-) and water ...
Escribano BM, Molina A, Valera M, Tovar P, Agüera EI, Santisteban R, Vivo R, Agüera S, Rubio MD.The novel aim of this study was to describe the reference values of different haematological and biochemical parameters in the Spanish purebred horse (Andalusian, SPB) in each of the stages of a programmed exercise on a treadmill system, and to establish heritability and genetic correlations for these haematological and biochemical parameters. For this, 94 young SPB male horses (4.22 ± 2.27 years old) were used. An increasing intensity exercise test at 4, 5, 6 and 7 m/s was carried out on a treadmill (6% inclination). Total red blood cells, total white blood cells, neutrophils and lymphocytes...
Trachsel DS, Schwarzwald CC, Bitschnau C, Grenacher B, Weishaupt MA.Atrial natriuretic peptide (ANP) and cardiac troponin I (cTnI) serve as biomarkers for increased cardiac pressure/volume loading and for myocardial stress or damage. The objective was to describe the time course of plasma ANP concentrations (CpANP) and plasma cTnI concentrations (CpcTnI) in horses with mitral regurgitation (MR) compared to healthy horses at rest and after exercise, and to describe the relationship of CpANP with cardiac dimensions and intracardiac pressures. Methods: 15 healthy Warmblood horses and 7 Warmblood horses with MR. Methods: Cardiac dimensions at rest were measured us...
La Gerche A, Daffy JR, Mooney DJ, Forbes G, Davie AJ.It has been observed that microbubbles may pass through the pulmonary circulation of dogs and humans during exercise. In humans, this phenomenon has been associated with lower pulmonary artery pressures, enhanced right ventricular function and greater exercise capacity. In the exercising Thoroughbred horse, extraordinarily high cardiac outputs exert significant pulmonary vascular stresses. The aim of this study was to determine, using contrast echocardiography, whether Thoroughbred horses performing strenuous exercise developed pulmonary transit of agitated contrast microbubbles (PTAC). At res...
Cywinska A, Witkowski L, Szarska E, Schollenberger A, Winnicka A.Serum amyloid A (SAA) is the major acute phase protein in horses. Its concentration increases in various pathologies but also in response to prolonged, strenuous effort. The purpose of this study was to establish whether routine race and endurance training produces changes in the SAA level in Arabian horses. Additionally, the differences between SAA response in experienced endurance horses and endurance horses that were beginning their career were investigated. Results: There were no changes in SAA concentrations after race training and endurance training in experienced horses. In horses that ...
van Weeren PR, Denoix JM.Juvenile osteochondral conditions (JOCC) have a major impact on the equine industry and include many musculoskeletal disorders of the young horse, of which osteochondrosis (OC) is the most prominent. The Breeding, Osteochondral Status and Athletic Career (BOSAC) project is the first large, comprehensive, longitudinal field study on the subject conducted in three breeds of performance horses (Thoroughbreds, Standardbred Trotters and Warmbloods) that were monitored in their natural environment where they were reared under common field conditions. The BOSAC study used a radiographic protocol desi...
Peters LW, Smiet E, de Sain-van der Velden MG, van der Kolk JH.In particular branched-chain amino acids might limit muscle protein loss in pathological conditions. Little is known on basic amino acid utilization of muscle in horses. Objective: To assess amino acid utilization by the hindlimb of horses at rest and following low intensity exercise. ANIMALS & METHODS: Amino acid uptake by the hindlimb was investigated using the arteriovenous difference technique. Blood from six warmblood mares (mean age 12 ± 3 (SD) years and weighing 538 ± 39 kg) was collected simultaneously from the (transverse) facial artery and from the caudal vena cava. Food was wi...
Tsubone H, Hanafusa M, Endo M, Manabe N, Hiraga A, Ohmura H, Aida H.The present study aimed to clarify changes of oxidative stress and antioxidative functions in treadmill-exercised Thoroughbred horses (n=5, 3 to 7 years old), using recently developed techniques for measurement of serum d-ROMs for oxidative stress, and BAP for antioxidative markers. Also, the effect of nasogastric administration of hydrogen-rich water (HW) or placebo water preceding the treadmill exercise on these parameters was examined. Each horse was subjected to a maximum level of treadmill exercise in which the horses were exhausted at an average speed of 13.2 ± 0.84 m/sec. Blood samples...
Kim H, Lee T, Park W, Lee JW, Kim J, Lee BY, Ahn H, Moon S, Cho S, Do KT, Kim HS, Lee HK, Lee CK, Kong HS, Yang YM, Park J, Kim HM, Kim BC, Hwang S....The modern horse (Equus caballus) is the product of over 50 million yrs of evolution. The athletic abilities of the horse have been enhanced during the past 6000 yrs under domestication. Therefore, the horse serves as a valuable model to understand the physiology and molecular mechanisms of adaptive responses to exercise. The structure and function of skeletal muscle show remarkable plasticity to the physical and metabolic challenges following exercise. Here, we reveal an evolutionary layer of responsiveness to exercise-stress in the skeletal muscle of the racing horse. We analysed differentia...
Nolen-Walston RD, Harris M, Agnew ME, Martin BB, Reef VB, Boston RC, Davidson EJ.To determine whether there are important differences relating to seasonality of signs or clinical features between subtypes of inflammatory airway disease (IAD) in horses caused by neutrophilic and eosinophilic-mastocytic inflammation having dissimilar etiopathologic pathways. Methods: Retrospective case series. Methods: 98 horses. Methods: Data were compiled from medical records of horses examined because of poor performance from 2004 through 2010. Horses underwent a standardized high-speed treadmill test, lameness evaluation, cardiac evaluation, and postexercise bronchoalveolar lavage (BAL)....
Zeyner A, Romanowski K, Vernunft A, Harris P, Kienzle E.Based on a series of exercise tests which included the estimation of sweat losses, this article proposes a novel sweat scoring system for exercising horses. This provides a practical estimate of individual animal exercise-induced sweat losses, based on visible appearance of sweat on the coat after work, which takes into account the effect of various influencing factors. In terms of accuracy and flexibility, the score seems to provide advantages over estimates based on current general recommendations from reference books. Additional studies are needed to validate this scoring system and its use...
Kim W, McArdle BH, Kawcak CE, McIlwraith CW, Firth EC, Broom ND.To investigate histomorphometric changes in the cartilage and subchondral bone of the third carpal bone associated with conditioning exercise in young Thoroughbreds. Methods: Nine 18-month-old Thoroughbreds. Procedures-Both third carpal bones of 9 horses (4 exercised spontaneously at pasture only and 5 given additional conditioning exercise beginning at a mean age of 3 weeks) were evaluated. Histomorphometric variables (hyaline and calcified cartilage thickness and collagen orientation; vascular channel area, number, and orientation; and osteochondral junction rugosity) of the third carpal bon...
Kitaoka Y, Endo Y, Mukai K, Aida H, Hiraga A, Takemasa T, Hatta H.To evaluate the effects of a single incremental exercise test (IET) on mRNA expression and protein content of monocarboxylate transporter (MCT) 1 and MCT4 in the gluteus medius muscle of Thoroughbreds. Methods: 12 Thoroughbreds (6 males and 6 females; age, 3 to 4 years). Methods: Horses underwent an IET before and after 18 weeks of high-intensity exercise training (HIT). Horses were exercised at 90% of maximal oxygen consumption for 3 minutes during the initial 10 weeks of HIT and 110% of maximal oxygen consumption for 3 minutes during the last 8 weeks of HIT. Gluteus medius muscle biopsy spec...
Tofé E, Muñoz A, Castejón F, Trigo P, Castejón-Riber C, Gómez-Díez M, Riber C.The influence of hydration prior to exercise on hormonal response to exercise was investigated in horses, divided into two groups, control (n=11) and dehydrated (n=53). They were also classified according to their body weight in A (≈ 350 kg), B (351-450 kg) and C (≈ 451 kg). Horses covered 60 m on a track pulling a carriage loaded with 2, 2.25 and 2.5 times their body weight, for A, B and C. Blood samples were taken at rest, after exercise and during recovery. Electrolytes, albumin, renin, angiotensin and aldosterone concentrations were measured. Dehydrated and control horses had higher al...
Holden-Douilly L, Pourcelot P, Desquilbet L, Falala S, Crevier-Denoix N, Chateau H.Longitudinal sliding of horse's hooves at the beginning of stance can affect both performance and orthopaedic health. The objective of this study was to compare two measurement methods for quantifying hoof slip distances at training trot. The right front hoof of four French Trotters was equipped with an accelerometer (10 kHz) and kinematic markers. A firm wet sand track was equipped with a 50 m calibration corridor. A high-frequency camera (600 Hz) was mounted in a vehicle following each horse trotting at about 7 m/s. One of the horses was also trotted on raw dirt and harrowed dirt tracks. Lon...
Pedersen PJ, Kanters JK, Buhl R, Klaerke DA.Cardiac repolarization, measured as QT and Tpeak to Tend (TpTe) intervals on the ECG, is important, as irregularities caused by diseases, ventricular hypertrophy, drugs and genetic defects can trigger arrhythmias which predispose human patients to syncope and sudden cardiac death. In horses, repolarization is not well described and therefore QT analysis cannot yet be used diagnostically. Therefore, we sought to describe reference values for the normal QT and TpTe intervals in Standardbreds and to determine the best method for heart rate (HR) correction. Methods: 30 Standardbreds. Methods: QT a...
Cathcart MP, Love S, Sutton DG, Reardon RJ, Hughes KJ.Analyses of exhaled breath (EB) and exhaled breath condensate (EBC) are non-invasive modalities for assessing the lower airways but these methods have not been applied to Thoroughbred racehorses in training. The aims of this study were to determine whether EB and EBC could be obtained from Thoroughbred racehorses in the field and to investigate the effects of exercise per se and during different ambient temperatures and humidity on exhaled concentrations of nitric oxide (eNO), carbon monoxide (eCO) and EBC pH. EB and EBC samples were obtained from 28 Thoroughbred racehorses pre- and post-exerc...
von Lewinski M, Biau S, Erber R, Ille N, Aurich J, Faure JM, Möstl E, Aurich C.Although some information exists on the stress response of horses in equestrian sports, the horse-rider team is much less well understood. In this study, salivary cortisol concentrations, heart rate (HR) and heart rate variability (HRV), SDRR (standard deviation of beat-to-beat interval) and RMSSD (root mean square of successive beat-to-beat intervals) were analysed in horses and their riders (n=6 each) at a public performance and an identical rehearsal that was not open to the public. Cortisol concentrations increased in both horses and riders (P<0.001) but did not differ between performan...
Whitton RC, Mirams M, Mackie EJ, Anderson GA, Seeman E.Bone remodelling is inhibited by high repetitive loading. However, in subchondral bone of racehorses in training, eroded surface doubled in association with fatigue fracture and there was greater surrounding trabecular bone volume suggesting trabecular modelling unloads the bone focally, allowing damage repair by remodelling. Background: Remodelling replaces damaged bone with new bone but is suppressed during high magnitude repetitive loading when damage is most likely. However, in cortical bone of racehorses, at sites of fatigue fracture, focal porosity, consistent with remodelling, is observ...
Van Erck-Westergren E, Franklin SH, Bayly WM.Given that aerobic metabolism is the predominant energy pathway for most sports, the respiratory system can be a rate-limiting factor in the exercise capacity of fit and healthy horses. Consequently, respiratory diseases, even in mild forms, are potentially deleterious to any athletic performance. The functional impairment associated with a respiratory condition depends on the degree of severity of the disease and the equestrian discipline involved. Respiratory abnormalities generally result in an increase in respiratory impedance and work of breathing and a reduced level of ventilation that c...
Rubin CT, Seeherman H, Qin YX, Gross TS.Distributions of normal strain, shear strain, and strain energy density (SED) were determined across the midshaft of the third metacarpal (MCIII, or cannon bone) of 3 adult thoroughbred horses as a function of speed and gait. A complete characterization of the mechanical demands of the bone made through the stride and from mild through the extremes of locomotion was possible by using three 3-element rosette strain gauges bonded at the diaphyseal midshaft of the MCIII and evaluating the strain output with beam theory and finite element analysis. Mean ± sd values of normal strain, shear strain,...
Fonseca RG, Kenny DA, Hill EW, Katz LM.Few noninvasive measures associated with performance assessment are available for racehorse trainers. Evaluation of body composition of superior human sprinters has revealed a lower fat mass (FM), percentage (%) fat and greater fat-free mass (FFM), but to date there have been few studies evaluating this in racehorses. Objective: To determine the effects of age, gender and training on body composition and the relationship between body composition, physiological measurements and performance in Thoroughbred racehorses. Methods: At 2, 5 and 8 months of training, rump fat thickness (RFT) was ultras...
Kȩdzierski W, Pluta M.Every year a new group of young Polish Konik colts are separated from the forest herds to be trained in the stable breeding system. The aim of this study was to evaluate how the young Polish Konik horses who had been born in a forest reserve adapt to and tolerate draft work. Two groups of 6 horses each were studied: (a) 3- to 4-year-old colts and (b) 7- to 13-year-old stallions. An effort response was estimated by heart rate (HR) registration and biochemical analysis of hematocrit; blood lactic acid (LA) level; and plasma concentration of glucose, triacylglycerols, uric acid, total protein, an...
Reed SR, Jackson BF, Wood JL, Price JS, Verheyen KL.The aim of this study was to identify exercise-related risk factors for carpal and metacarpo- and metatarso-phalangeal (MCP/MTP) joint injury occurrence in young Thoroughbreds in flat race training. In a 2-year prospective cohort study, daily exercise and joint injury data were collected from horses in 13 training yards in England. Four injury categories were defined: (1) localised to a carpal or MCP/MTP joint based on clinical examination and/or use of diagnostic analgesia with no diagnostic imaging performed; (2) localised to a carpal or MCP/MTP joint with no abnormalities detected on diagno...
Snow DH, Baxter P, Rose RJ.An investigation into fibre composition and glycogen depletion pattern within the middle gluteal of 16 horses participating in an 80 km endurance ride was carried out. Although the proportion of slow twitch high oxidative (ST) fibres in the horses varied between 7 and 38 per cent, it was found that the horses with the highest proportion of these fibres usually had the best performance records. The cross-sectional area of the fast twitch low oxidative (FT) fibres was greatest, with the ST and fast twitch high oxidative (FTH) being similar in size. Most marked histological evidence of glycogen d...
Pirrone F, Albertini M, Clement MG, Lafortuna CL.The aim of this study was to investigate and quantify respiratory mechanical dysfunctions in Standardbred horses with both poor performance and bronchoalveolar lavage fluid cytology characteristic of inflammatory airway disease (IAD). A control group of healthy Standardbred horses was compared. Respiratory mechanics and breathing pattern were examined at rest and during hyperventilation induced using a rebreathing method. At rest, respiratory mechanics and breathing pattern were superimposable in both groups. In IAD horses, rebreathing increased ventilation, with larger tidal volumes and lower...
Perez-Moreno CI, Couëtil LL, Pratt SM, Ochoa-Acuña HG, Raskin RE, Russell MA.The objective was to quantify the effect of furosemide and carbazochrome on exercise-induced pulmonary hemorrhage (EIPH) in Standardbred horses using red blood cell count and hemoglobin concentration in bronchoalveolar lavage (BAL) fluid. Six healthy Standardbred horses with prior evidence of EIPH performed a standardized treadmill test 4 h after administration of placebo, furosemide, or furosemide-carbazochrome combination. Red blood cell (RBC) counts and hemoglobin concentrations were determined on the BAL fluid. The RBC count in BAL ranges were (2903-26,025 cells/microL), (45-24,060 cells/m...
Uldahl M, Christensen JW, Clayton HM.Riders need core stability to follow and guide the horse's movements and avoid giving unintended or conflicting signals. This study evaluated the rider's performance of exercises on a gymnastic ball with on-horse performance and indicators of stress in the horse. Twenty experienced riders were scored performing three exercises on a gymnastic ball and for quality and harmony when riding based on evaluation of video recordings in which conflict behaviours were evident. The horse's heart rate and number of conflict behaviors during the riding test and cortisol levels after completion of the test ...
Urschel KL, Geor RJ, Waterfall HL, Shoveller AK, McCutcheon LJ.Providing protein or amino acid mixtures in combination with glucose to post exercise in man has resulted in increases in the post feeding insulin response and in muscle glycogen and protein synthesis rates. However, whether protein and/or amino acids can modify the post exercise insulin responses in horses remains to be fully elucidated. Objective: To determine whether whey protein or leucine addition to a glucose solution affects the post gavage plasma insulin, glucose and amino acid responses in horses and whether these responses are different following a period of exercise vs. rest. Method...
Antunes FN, Pinho ASD, Kleiner AFR, Salazar AP, Eltz GD, de Oliveira Junior AA, Cechetti F, Galli M, Pagnussat AS.Hippotherapy is often carried out for the rehabilitation of children with Cerebral Palsy (CP), with the horse riding at a walking pace. This study aimed to explore the immediate effects of a hippotherapy protocol using a walk-trot pace on spatio-temporal gait parameters and muscle tone in children with Bilateral Spastic CP (BS-CP). Ten children diagnosed with BS-CP and 10 healthy aged-matched children (reference group) took part in this study. The children with BS-CP underwent two sessions of hippotherapy for one week of washout between them. Two protocols (lasting 30min) were applied on separ...
Nanua P, Waldron KJ.In this paper, the dynamics of quadruped trot, gallop, and bound will be examined using a simple model for the quadruped. The body of the quadruped is modeled as a uniform bar and the legs are modeled by massless springs. It will be shown that symmetry can be used to study the locomotion of this system. Using symmetry, a technique will be developed to obtain periodic solutions for each of the gaits of the quadruped model. These periodic solutions will be computed at various speeds. The energy levels will be compared for each of the gaits. The exchange of energy between its different forms will...
Piccione G, Assenza A, Fazio F, Percipalle M, Caola G.BACKGROUND: The serotonergic system is associated with numerous brain functions, including the resetting of the mammalian circadian clock. The synthesis and metabolism of 5-HT in the brain increases in response to exercise and is correlated with high levels of blood-borne tryptophan (TRP). The present investigation was aimed at testing the existence of a daily rhythm of TRP and 5-HT in the blood of athletic horses. METHODS: Blood samples from 5 Thoroughbred mares were collected at 4-hour intervals for 48 hours (starting at 08:00 hours on day 1 and finishing at 4:00 on day 2) via an intravenous...
Lanovaz JL, Clayton HM.Estimations of segmental inertial parameters are required for true inverse dynamics calculations during the swing phase of locomotion. This study attempts to quantify the effect of inertial parameter errors on inverse dynamic solutions. Swing phase forelimb net joint moments and powers at the trot (mean +/- s.d 3.03 +/- 0.16 m/s) were calculated with sagittal plane kinematic data from 5 Dutch Warmbloods using inertial parameters based on published regression equations for the breed. Significant peaks in the net moment and power curves for each forelimb joint were identified and measured. Net j...
Best R, Standing R.Global positioning systems (GPS) have recently been shown to reliably quantify the spatiotemporal characteristics of Polo, with the physiological demands of Polo play at low- and high-goal levels also investigated. This study aims to describe the spatiotemporal demands of Polo across 0-24 goal levels. A player-worn GPS unit was used to quantify distance, speed and high-intensity activities performed. Data were divided into chukkas and five equine-based speed zones, grouped per cumulative player handicap and assessed using standardized mean differences. Average distance and speed per chukka inc...
Pourmohammad R, Mohri M, Seifi HA, Sardari K.Limited information existed on performance tests in Iranian Arab horses. Objective: The objective of this study is to investigate time related changes of cardiac troponin I (cTnI), atrial natriuretic peptide (ANP) and oxidative/antioxidant biomarkers in the serum of Arabian horses before and after regular training. Methods: Blood samples were collected from jugular vein of 25 Arabian horses before exercise; 5 h and 18 h after exercise and used to measure the cTnI, ANP, malondialdehyde (MDA), ferric reducing antioxidant power (FRAP), glutathione peroxidase (GPX), and superoxide dismutase (SOD) ...
Trachsel DS, Bitschnau C, Waldern N, Weishaupt MA, Schwarzwald CC.Frequent supraventricular or ventricular arrhythmias during and after exercise are considered pathological in horses. Prevalence of arrhythmias seen in apparently healthy horses is still a matter of debate and may depend on breed, athletic condition and exercise intensity. Objective: To determine intra- and interobserver agreement for detection of arrhythmias at rest, during and after exercise using a telemetric electrocardiography device. Methods: The electrocardiogram (ECG) recordings of 10 healthy Warmblood horses (5 of which had an intracardiac catheter in place) undergoing a standardised ...
Malikides N, Hughes KJ, Hodgson JL.To determine whether percentages of neutrophils in tracheal aspirate (TA) samples collected from racehorses are increased after exercise and whether interpretation of results from TA samples taken before and after exercise agree. Methods: Case series of 40 young Thoroughbred and Standardbred racehorses in race training presented for evaluation of poor performance. Methods: TA samples were collected endoscopically from racehorses presented for poor performance 24 h before and 1 to 2 h after high speed treadmill exercise testing. Aliquots of the retrieved fluid were cytocentrifuged and smears we...
Connysson M, Muhonen S, Jansson A.This study investigated the effects of transport and diet on metabolic response during a subsequent race-like test in Standardbred horses in training fed a forage-only diet and a 50:50 forage:oats diet. Six trained and raced Standardbred trotter mares were used. Two diets, 1 forage-only diet (FONLY) and 1 diet with 50% of DM intake from forage and 50% from oats (FOATS), were fed for two 29-d periods in a crossover design. At Day 21, the horses were subjected to transport for 100 km before and after they performed an exercise test (transport test [TT]). At Day 26, the horses performed a control...
Schmutz A, Chèze L, Jacques J, Martin P.With the emergence of numerical sensors in sports, there is an increasing need for tools and methods to compute objective motion parameters with great accuracy. In particular, inertial measurement units are increasingly used in the clinical domain or the sports one to estimate spatiotemporal parameters. The purpose of the present study was to develop a model that can be included in a smart device in order to estimate the horse speed per stride from accelerometric and gyroscopic data without the use of a global positioning system, enabling the use of such a tool in both indoor and outdoor condi...
Olivier A, Viseu JP, Vignais N, Vuillerme N.Horseback riding requires the ability to adapt to changes in balance conditions, to maintain equilibrium on the horse and to prevent falls. Postural adaptation involves specific sensorimotor processes integrating visual information and somesthesic information. The objective of this study was to examine this multisensorial integration on postural control, especially the use of visual and plantar information in static (stable) and dynamic (unstable) postures, among a group of expert horse rider women (n = 10) and a group of non-athlete women (n = 12). Postural control was evaluated through the c...
Little CB, Ghosh P, Rose R.Articular cartilage degeneration in the middle carpal joint is a common problem in racing horses. This study evaluated the effect of exercise on the in-vitro synthesis of the large aggregating proteoglycans (aggrecan) and two small proteoglycans, biglycan and decorin, in articular cartilage taken from three weight bearing regions of the third carpal bone of horses which were subjected to moderate or strenuous exercise. Twelve Standardbred horses free from clinical and radiographic disease of the middle carpal joint were subjected to an 8 week moderate exercise program. The horses were then ran...
Harkins JD, Beadle RE, Kamerling SG.The running abilities of 25 Thoroughbred racehorses were measured at distances of 1200, 1600 and 20000 m. Various physiological variables were measured subsequently on the treadmill and correlated with running speed. There was a negative correlation for running speed with the velocity (VLa4) and work rate (WLa4) at which blood lactate reaches a steady-state concentration of 4 mmol/litre and a positive correlation with peak plasma lactate, suggesting that plasma lactate concentrations of faster horses rise more rapidly and to higher levels than do those of slower horses. The correlation between...
Kohn CW, Hinchcliff KW.Physiological data were collected during two 3-day-event competitions: one (H) held in hot and the other (CL) in cool conditions. During H, ambient temperature and relative humidity were 2.5 degrees C-35 degrees C and 74-36% respectively, while during CL, ambient temperature and relative humidity were 7.8 degrees C-8.3 degrees C and 46%-41%, respectively. Rectal temperature, heart and respiratory rates were recorded on arrival at the event, at the end of Phase C and 6 min later, at the end of Phase D and for 30 min at 10 min intervals after each horse finished Phase D (Recovery Period). Becaus...
van Ginneken MM, de Graaf-Roelfsema E, Keizer HA, van Dam KG, Wijnberg ID, van der Kolk JH, van Breda E.To investigate the effects of exercise on activation of mitogen-activated protein kinase (MAPK) signaling proteins in horses. Methods: 6 young trained Standardbred geldings. Methods: Horses performed a 20-minute bout of exercise on a treadmill at 80% of maximal heart rate. Muscle biopsy specimens were obtained from the vastus lateralis and pectoralis descendens muscles before and after exercise. Amount of expression and intracellular location of phosphospecific MAPK pathway intermediates were determined by use of western blotting and immunofluorescence staining. Results: Exercise resulted in a...
Clayton HM, Schamhardt HC, Hobbs SJ.In this study, sagittal plane ground reaction forces (GRFs) in ridden elite dressage horses performing 'collected trot' and in 'passage' over ground were determined. In-ground force plates captured GRF data from four Dutch Warmblood and four Lusitano horses ridden by their trainers. At least three stance phases were analysed for forelimbs and hind limbs per horse. The variables extracted were vertical and longitudinal (braking, propulsive) force maxima, their times of occurrence and the respective impulses for forelimbs and hind limbs. Lusitanos had lower vertical impulses than Dutch Warmblood...
White A, Reyes A, Godoy A, Martínez R.1. Packed cell volume (PCV), blood glucose, total plasma proteins (TPP) and plasma electrolytes, osmolality, cortisol and aldosterone alterations produced by transport and racing, were investigated in race horses. 2. Plasma cortisol, sodium and blood glucose, found after transport, were higher, while aldosterone was lower than control levels. 3. After racing, PCV, blood glucose, TPP and plasma cortisol, sodium and osmolality were higher than control, while chloride diminished and aldosterone returned to control values. 4. These results demonstrate that transport and racing are different kinds ...
Echigoya Y, Morita S, Itou T, Sakai T.To evaluate effects of extracellular lactate on viability, shape change, lactate metabolism, and reactive oxygen species (ROS) production in equine polymorphonuclear leukocytes (PMNs). Methods: PMNs isolated from equine venous blood samples. Methods: PMNs were incubated with 0 to 300mM lactate for 30 minutes before each experiment. Viability was assessed via trypan blue exclusion. Shape change was assessed via flow cytometry and light microscopy. Relative quantification of monocarboxylic acid transporter and lactate dehydrogenase lactate dehydrogenase (LDH) isotype mRNAs was performed with a r...
Xing J, Xie L, Qi X, Liu G, Akhtar MF, Li X, Bou G, Bai D, Zhao Y, Dugarjaviin M, Zhang X.The proliferation and differentiation of skeletal muscle satellite cells (SCs) are necessary for the development of mature skeletal muscle. Leucine (Leu) is both an essential amino acid (EAA) and a branched-chain amino acid (BCAA), which has attracted worldwide attention due to its ability to repair and become new fibers. We separated the equine SCs into the control group (CON) and the Leu-supplemented group (LEU), which the cells were cultured in Leu-deprived and Leu-supplemented media respectively. We combined the transcriptome (RNA-Seq) and quantitative proteome (TMT) profiling analyses on ...
Miranda MC, Queiroz-Neto A, Silva-Júnior JR, Pereira MC, Soares OA, Borghi RT, Ferraz GC.To compare five different protocols for estimating the lactate minimum speed (LMS) with that for estimating the maximal lactate steady state (MLSS) in Arabian horses, in order to obtain a more rapid method for monitoring aerobic capacity and prescribing training schedules. Methods: Eight purebred Arabian horses were conditioned to exercise on a treadmill for 12 days then submitted to three to five exercise sessions to determine the MLSS. Blood samples were collected from a jugular catheter at specific intervals for measurement of lactate concentrations. The MLSS was the velocity maintained dur...
Mélo SK, Diniz AI, de Lira VL, de Oliveira Muniz SK, da Silva GR, Manso HE, Manso Filho HC.Oxidative stress has been correlated with pathologies that impair the performance of athlete horses. The aim of this study was to assess the effects of supplementation with a mixture of polyunsaturated oil and vitamin E on the antioxidant and haematological biomarkers of horses. Horses under maintenance care (n = 6) and horses in training (n = 10) received 100 and 300 ml of the oil mixture respectively. Supplementation was provided for a period of 8 weeks, together with isocaloric inclusion. Blood samples were collected at three time periods (pretest, after 4 weeks and after 8 weeks) t...
Cywińska A, Wyszyńska Z, Górecka R, Szarska E, Witkowski L, Dziekan P, Winnicka A, Schollenberger A.Strenuous exercise is recognized as a stress, which may induce functional immunodeficiency and increase individual susceptibility to infection. It has been shown in equine athletes, that alterations in leukocyte functions occur after moderate and submaximal exertion, however, no data deal with the effect of extreme physical exertion. In this study, we evaluated leukocyte functions (neutrophil oxidative burst and lymphocyte proliferation activity in response to mitogens) in horses following the CEI 3* 162 km endurance ride. Exercise-induced stress was manifested as neutrophilic leukocytosis and...
Ducharme NG, Hackett RP, Gleed RD, Ainsworth DM, Erb HN, Mitchell LM, Soderholm LV.We hypothesized that changes in pleural pressure induced by resistive breathing would affect transmural pulmonary artery, pulmonary capillary, and pulmonary wedge pressures. Seven horses were assigned to exercise with each of 4 upper respiratory resistive loads in random order at intervals of at least 2 days: 1) control--no added resistive loads; 2) inspiratory resistive load (Iobst)--left laryngeal hemiplegia; 3) expiratory resistive load (Eobst)--one-way valve in the right nostril; and 4) combined inspiratory and expiratory resistive loads (CIEobst)--left nostril occlusion. On each occasion,...
Morgan K, Funkquist P, Nyman G.The aim of this study was to study the physiological, especially thermoregulatory, responses during intense exercise in the clipped horse compared to the horse with winter coat. Six Standardbred trotters were studied before and after clipping. They performed an inclined incremental high intensity treadmill exercise test and were monitored during recovery. The clipped horse differed significantly (ANOVA) during exercise as compare to coated: less increase in central venous blood temperature, higher skin surface temperature, greater difference skin to ambient temperature and higher rate of nonev...
Szemplinski KL, Thompson A, Cherry N, Guay K, Smith WB, Brady J, Jones T.The objective of this study was to determine if transportation and exercise stress in horses affect the microflora populations in the equine hindgut. Four horses were subjected to three transport periods (0, 3, and 6 hours) with a 7-d rest period between each transport. Horses were fed 0.91 kg/day of Purina Impact All Stages 12% and had ad libitum access to Cynodon dactylon (Coastal Bermudagrass) hay. Fecal samples were collected before (0 hours) and after (48 hours) transport. In addition, three horses underwent a different standardized exercise test with a 7-d rest period between each ex...
