Analyze Diet

Topic:Food Safety

Food safety in horses encompasses practices and standards aimed at ensuring the health and well-being of horses through the management of their dietary intake. This includes the assessment and control of potential contaminants and pathogens in equine feed and water sources. Key concerns in equine food safety include the presence of mycotoxins, pesticide residues, and bacterial pathogens such as Salmonella and Clostridium botulinum. Proper feed storage, sourcing, and preparation are integral to preventing foodborne illnesses in horses. This page compiles peer-reviewed research studies and scholarly articles that explore the detection, impact, and management of food safety issues in equine nutrition.
Safety of hexamethylene tetramine for pigs, poultry, bovines, sheep, goats, rabbits and horses.
EFSA journal. European Food Safety Authority    February 14, 2020   Volume 18, Issue 2 e06012 doi: 10.2903/j.efsa.2020.6012
Bampidis V, Azimonti G, Bastos ML, Christensen H, Dusemund B, Kouba M, Kos Durjava M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) was asked to deliver a scientific opinion on hexamethylene tetramine (HMTA) for pigs, poultry, bovines, sheep, goats, rabbits and horses. In 2015, the FEEDAP Panel delivered an opinion on the safety and efficacy of HMTA. In that opinion, the Panel noted that the residues of HMTA in the silage amounted to about 5% of the inclusion level of 600 mg HMTA/kg fresh ensiling material. Since the HMTA residues in silage were analysed as formaldehyde, it was not possible to...
Effect of food contamination and collection material in the measurement of biomarkers in saliva of horses.
Research in veterinary science    January 8, 2020   Volume 129 90-95 doi: 10.1016/j.rvsc.2020.01.006
Contreras-Aguilar MD, Hevia ML, Escribano D, Lamy E, Tecles F, Cerón JJ.This study aims to evaluate the effect of the presence of food and the material used in a panel of biomarkers in saliva of horses. For the food effect study, clean saliva was incubated with a known amount of food consisting of oats, hay or grass. Significant changes were observed when saliva was incubated with oats for total protein (P = .050) and phosphorus (P = .008), with grass for total protein (P = .037), salivary alpha-amylase (sAA, P = .018), total esterase (TEA, P = .018), butyrilcholinesterase (BChE, P = .037), adenosine deaminase (ADA, P = .037), and total bilirubin (P...
Assessment of the application for renewal of authorisation of Yea-Sacc® (Saccharomyces cerevisiae) for horses.
EFSA journal. European Food Safety Authority    December 19, 2019   Volume 17, Issue 12 e05918 doi: 10.2903/j.efsa.2019.5918
Bampidis V, Azimonti G, de Lourdes Bastos M, Christensen H, Dusemund B, Kouba M, Kos Durjava M, López-Alonso M, López Puente S, Marcon F, Mayo B....Yea-Sacc is an additive based on a live preparation of a strain of intended for use as a zootechnical additive (digestibility enhancer). The current assessment is performed in the context of the renewal of the authorisation of the feed additive; however, the applicant proposed also to lower (6.5 × 10 colony forming unit (CFU)/kg of complete feedingstuff) the minimum dose of the additive when used in feed for horses. The additive is produced in a powder (Yea-Sacc) and in a prills (Yea-Sacc_TS) forms. The Panel considered that the additive currently on the market complies with the existing ...
Production technology, nutritional, and microbiological investigation of traditionally fermented mare milk (Chigee) from Xilin Gol in China.
Food science & nutrition    December 9, 2019   Volume 8, Issue 1 257-264 doi: 10.1002/fsn3.1298
Guo L, Xu WL, Li CD, Ya M, Guo YS, Qian JP, Zhu JJ.Mare milk originated from female horses, known as mares, to feed their foals during lactation. The health-promoting characteristics of traditionally fermented mare milk (Chigee) are well known for the function of clinic treatment in the traditional Mongolian medicine. This study was conducted to investigate the production technology of Chigee and to evaluate the nutritional and microbiological characteristics of mare milk and Chigee based on 188 samples. The nutritional analysis of mare milk and Chigee indicated that lactose significantly decreased from 6.95 ± 0.45% to 2.82 ± 1.65% and a...
Clostridioides (Clostridium) Difficile in Food-Producing Animals, Horses and Household Pets: A Comprehensive Review.
Microorganisms    December 9, 2019   Volume 7, Issue 12 667 doi: 10.3390/microorganisms7120667
Kachrimanidou M, Tzika E, Filioussis G. is ubiquitous in the environment and is also considered as a bacterium of great importance in diarrhea-associated disease for humans and different animal species. Food animals and household pets are frequently found positive for toxigenic without exposing clinical signs of infection. Humans and animals share common ribotypes (RTs) suggesting potential zoonotic transmission. However, the role of animals for the development of human infection due to remains unclear. One major public health issue is the existence of asymptomatic animals that carry and shed the bacterium to the environment, an...
Assessment of the application for renewal of authorisation of Biosprint® (Saccharomyces cerevisiae MUCL 39885) for dairy cows and horses.
EFSA journal. European Food Safety Authority    November 28, 2019   Volume 17, Issue 11 e05915 doi: 10.2903/j.efsa.2019.5915
Bampidis V, Azimonti G, Bastos ML, Christensen H, Dusemund B, Kouba M, Kos Durjava M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....Following a request from the European Commission, EFSA was asked to deliver a scientific opinion on the assessment of the application for renewal of authorisation of the product Biosprint ( MUCL 39885) as a feed additive for dairy cows and horses. is considered by EFSA to have qualified presumption of safety (QPS) status. The applicant has provided data demonstrating that the additive currently in the market complies with the conditions of authorisation. The Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) Panel confirms that the use of Biosprint under the current ...
Establishment and application of a 10-plex liquid bead array for the simultaneous rapid detection of animal species.
Journal of the science of food and agriculture    November 6, 2019   Volume 100, Issue 1 325-334 doi: 10.1002/jsfa.10042
Mei M, Chen R, Gao X, Cao Y, Weng W, Duan Y, Tan X, Liu Z.Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10-plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink. Results: This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg in bina...
Molecular Detection and Genetic Characterization of Toxoplasma gondii from Horses in Three Provinces of China.
Vector borne and zoonotic diseases (Larchmont, N.Y.)    March 26, 2019   Volume 19, Issue 9 703-707 doi: 10.1089/vbz.2018.2423
Ren WX, Zhang XX, Long CY, Zhao Q, Cheng T, Ma JG, Xu P, Hou G, Ni HB.Horse meat and milk are an important source of nutrition for many Chinese. Previous studies have shown a fairly high prevalence of infection in horses. However, there is no data concerning the molecular characterization of in horses in China. The present study tested 231 cervical lymph node samples of horses from northern China (97 from Jilin, 54 from Liaoning, and 80 from Xinjiang) for the presence of the B1 gene by seminested PCR. The B1-positive samples were genotyped at nine nuclear loci, that is, SAG1, alternative SAG2, SAG3, BTUB, c22-8, GRA6, c29-2, PK1, and an apicoplast locus, usin...
Assessment of the application for renewal of authorisation of Levucell SC (Saccharomyces cerevisiae CNCM I-1077) as a feed additive for lambs and horses.
EFSA journal. European Food Safety Authority    March 21, 2019   Volume 17, Issue 3 e05639 doi: 10.2903/j.efsa.2019.5639
Bampidis V, Azimonti G, Bastos ML, Christensen H, Dusemund B, Kouba M, Kos Durjava M, López-Alonso M, López Puente S, Marcon F, Mayo B, Pechová A....Levucell SC is the trade name for a feed additive based on viable cells of a strain . The product is currently authorised for use in feed for horses, lambs, dairy sheep and dairy goats, dairy cows and cattle for fattening. This opinion concerns the renewal of the authorisation of Levucell SC as a zootechnical additive for lambs and horses. is considered by EFSA to be suitable for the qualified presumption of safety (QPS) approach to establishing safety for the target species, consumers and the environment. The identity of the strain present in the additive was established. Accordingly, this s...
An optimized TaqMan real-time PCR method for authentication of ASINI CORII COLLA (donkey-hide gelatin).
Journal of pharmaceutical and biomedical analysis    March 15, 2019   Volume 170 196-203 doi: 10.1016/j.jpba.2019.03.028
Zhang W, Cui S, Cheng XL, Wei F, Ma S.In this study, probe/primers of high specificity and sensitivity were selected to analyze donkey-hide gelatin for donkey DNA and to look for horse, ox, and pig DNA as possible adulterants. The mitochondrial CO I genes in donkey, horse, and ox were selected as target sequences for design and synthesis of three pairs of specific probes and primers. In addition, eight pairs of probe/primers were obtained via literature search. Out of these eleven groups of probe/primers, those with the highest specificity and sensitivity were selected, which was fulfilled by the screening firstly with animal hide...
Sarcocystis bertrami in skeletal muscles of donkeys (Equus africanus asinus) from Southern Italy.
Veterinary parasitology, regional studies and reports    March 14, 2019   Volume 16 100283 doi: 10.1016/j.vprsr.2019.100283
Among the protozoa of the genus Sarcocystis (Apicomplexa; Sarcocystidae), Sarcocystis bertrami (syn. Sarcocystis fayeri) is an obligate intracellular parasite of donkeys and horses with worldwide distribution. Here, we report the detection of S. bertrami in naturally infected donkeys from southern Italy and describe their structure by light microscopy (LM) and transmission electron microscopy (TEM). Protozoal cysts were detected both morphologically and molecularly in skeletal muscles of 28.57% (40/140) donkeys. Mature cysts of S. bertrami were found in skeletal muscle measuring 31-102 μm l...
Ethics in animal breeding.
Reproduction in domestic animals = Zuchthygiene    November 27, 2018   Volume 53 Suppl 3 4-13 doi: 10.1111/rda.13335
Farstad W.Ethical breeding involves the use of healthy animals true to their species in behaviour and physical appearance, and when applicable, showing a sustainable performance. The concerns for the species/breed are essential parts of the breeding goals, including preservation of genetic resources within the species/breed, and the health and welfare of the individual animal. Ethical and welfare considerations were often not prioritized in developing new breeds of production or companion animals. As a result, animal breeding practices are increasingly becoming part of the debate on animal welfare. In c...
Pyrrolizidine alkaloids in commercial feedstuffs for horses.
Equine veterinary journal    November 9, 2018   Volume 51, Issue 4 495-499 doi: 10.1111/evj.13033
Rückert C, Emmerich I, Hertzsch R, Vervuert I.Pyrrolizidine alkaloids are secondary plant metabolites with hepatotoxic effect in humans and several animal species. In recent studies, foods such as herbal teas and honey have been found to be contaminated with pyrrolizidine alkaloids. Objective: The aim of this study was to identify and assess pyrrolizidine alkaloids in compound feeds manufactured for horses and containing either alfalfa or a blend of herbs. Methods: Forty-eight feed products for horses were included in the study. The feedstuffs were analysed for 28 selected pyrrolizidine alkaloids by liquid chromatography-mass spectrometry...
Detection of Salmonella from animal sources in South Africa between 2007 and 2014.
Journal of the South African Veterinary Association    November 7, 2018   Volume 89 e1-e10 doi: 10.4102/jsava.v89i0.1643
Gelaw AK, Nthaba P, Matle I.Retrospective laboratory-based surveillance was conducted on Salmonella serotypes isolated from various animal species from 2007 to 2014 at the Agricultural Research Council, Onderstepoort Veterinary Research Institute, South Africa. During the surveillance period, 1229 salmonellae isolations were recorded. Around 108 different serotypes were recovered from nine different food and non-food animal host species. The three most common serotypes were Salmonella entericasubspecies enterica serotype Heidelberg (n = 200), Salmonella enterica subspecies enterica serotype Enteritidis (n = 17...
Global distribution data for cattle, buffaloes, horses, sheep, goats, pigs, chickens and ducks in 2010.
Scientific data    October 30, 2018   Volume 5 180227 doi: 10.1038/sdata.2018.227
Gilbert M, Nicolas G, Cinardi G, Van Boeckel TP, Vanwambeke SO, Wint GRW, Robinson TP.Global data sets on the geographic distribution of livestock are essential for diverse applications in agricultural socio-economics, food security, environmental impact assessment and epidemiology. We present a new version of the Gridded Livestock of the World (GLW 3) database, reflecting the most recently compiled and harmonized subnational livestock distribution data for 2010. GLW 3 provides global population densities of cattle, buffaloes, horses, sheep, goats, pigs, chickens and ducks in each land pixel at a spatial resolution of 0.083333 decimal degrees (approximately 10 km at the equat...
Seroprevalence and risk factors of Toxoplasma gondii infection in horses in Jilin Province and Inner Mongolia Autonomous Region, northern China.
Acta tropica    July 30, 2018   Volume 187 119-123 doi: 10.1016/j.actatropica.2018.07.030
Zhang XX, Ren WX, Hou G, Liu Q, Yu TQ, Zhao Q, Ni HB.Toxoplasmosis is caused by the protozoon Toxoplasma gondii that can infect almost all warm-blooded hosts including horses and humans. Horse meat is one of the most important meat products for human consumption. However, data on seroprevalence of T. gondii in horses intended for human consumption in Northern China is scarce. Thus, a total of 614 serum samples were collected from Jilin Province and Inner Mongolia Autonomous Region (IMAR) between 2015 and 2017, and were detected the seroprevalence for T. gondii by indirect hemagglutination assay test. In the prevalence study, the overall seroprev...
[New aspects of non-waste use of secondary raw materials of horse breeding in Yakutia].
Voprosy pitaniia    July 13, 2018   Volume 87, Issue 4 87-92 doi: 10.24411/0042-8833-2018-10046
Slobodchikova MN, Vasilyeva VT, Ivanov RV, Lebedeva UM.In the diet of the population of Yakutia, the meat of 6-month-old and local herd horses is especially valued for its high nutritive value, excellent taste and dietary properties. In addition to meat, a number of other slaughter products, such as by-products, intestinal raw materials, internal fat, blood, etc., can be received during primary processing. Many of them are not fully used. The relevance of this work is to study the possibilities of waste-free use of the Yakut horse products. Biochemical investigation of the composition of the secondary products of slaughter (fat, blood, by-products...
Simultaneous identification of bovine and equine DNA in milks and dairy products inferred from triplex TaqMan real-time PCR technique.
Journal of dairy science    June 7, 2018   Volume 101, Issue 8 6776-6786 doi: 10.3168/jds.2018-14408
Guo L, Qian JP, Guo YS, Hai X, Liu GQ, Luo JX, Ya M.Koumiss is a popular dairy product in many lands, traditionally prepared from mare milk with spontaneous fermentation. Mare milk and its fermented derivates are more expensive than cow milk and its fermented derivates, and the possibility exists for producers and dealers to adulterate equine products with bovine items. In this work, we described the development of a triplex real-time PCR based on species-specific TaqMan probes for identification of bovine and equine DNA in milks and dairy products. In addition, a novel designed endogenous control was simultaneously amplified to eliminate possi...
Development of an immunochromatographic assay for the β-adrenergic agonist feed additive zilpaterol.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment    June 6, 2018   Volume 35, Issue 8 1519-1529 doi: 10.1080/19440049.2018.1463568
Shelver WL, Smith DJ.Zilpaterol is a β-adrenergic agonist feed additive approved in the United States to increase weight gain and improve feed efficiency of cattle. A zilpaterol immunochromatographic assay was developed as an economical and user-friendly rapid detection method for zilpaterol and validated using urine and tissue samples derived from animal studies. The assay sensitivity was 1.7-23.2 ng g or mL across a variety of feed and animal matrices and did not cross-react with clenbuterol or ractopamine. No sample pre-treatment of cattle and sheep urine was needed, but horse urine and feed required dilution;...
[New drugs for horses and production animals in 2017].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    May 4, 2018   Volume 46, Issue 2 95-100 doi: 10.15653/TPG-180300
Emmerich IU.In 2017, no new active pharmaceutical ingredients were released on the German market for horses or food-producing animals. Four established veterinary active pharmaceutical ingredients became available for additional species: the ectoparasitic Fluralaner (Exzolt®) of the isoxazoline group was additionally authorized for chickens, the macrolide antibiotics Gamithromycin (Zactran®) and Tulathromycin (Draxxin®) for sheep and the nonsteroidal anti-inflammatory drug Tolfenamic Acid (Tolfedol®) from the fenamate group for cattle and pigs. Additionally, one drug with a new combination of active i...
Equine feed contamination and toxicology.
Translational animal science    March 13, 2018   Volume 2, Issue 1 111-118 doi: 10.1093/tas/txy001
Bischoff K, Moiseff J.Feed as a cause of poisoning in horses can occur on small or large scales. It is challenging to work up cases of suspected feed contamination, but there are resources available to veterinarians and owners. Feed contamination can be chemical or biological. This article focuses on and provides examples of chemical feed contamination including misformulation, adulteration, and natural contaminants. Additionally, recommendations for feed sampling and diagnostic submission, including legal documentation, are included.
Surveillance and diagnosis of zoonotic foodborne parasites.
Food science & nutrition    November 12, 2017   Volume 6, Issue 1 3-17 doi: 10.1002/fsn3.530
Zolfaghari Emameh R, Purmonen S, Sukura A, Parkkila S.Foodborne parasites are a source of human parasitic infection. Zoonotic infections of humans arise from a variety of domestic and wild animals, including sheep, goats, cattle, camels, horses, pigs, boars, bears, felines, canids, amphibians, reptiles, poultry, and aquatic animals such as fishes and shrimp. Therefore, the implementation of efficient, accessible, and controllable inspection policies for livestock, fisheries, slaughterhouses, and meat processing and packaging companies is highly recommended. In addition, more attention should be paid to the education of auditors from the quality c...
High Occurrence of Methicillin-Resistant Staphylococcus aureus in Horses at Slaughterhouses Compared with Those for Recreational Activities: A Professional and Food Safety Concern?
Foodborne pathogens and disease    October 25, 2017   Volume 14, Issue 12 735-741 doi: 10.1089/fpd.2017.2300
Parisi A, Caruso M, Normanno G, Latorre L, Miccolupo A, Fraccalvieri R, Intini F, Manginelli T, Santagada G.The epidemiology of methicillin-resistant Staphylococcus aureus (MRSA) in horses and its zoonotic potential is poorly understood. The objective of this study is to provide data on the prevalence and genetic characteristics of MRSA isolated from horses on farms, at racecourses, and at slaughterhouses in Italy, using standard and molecular methods. In addition, we report the prevalence of MRSA in horse handlers. Among 388 horses tested by nasal swabs, 27 (7%) were positive for MRSA ST398 (t011, t899, t1255) and ST1 (t127). The prevalence of MRSA in horses tested at slaughterhouses was significan...
Risks for animal health related to the presence of zearalenone and its modified forms in feed.
EFSA journal. European Food Safety Authority    July 31, 2017   Volume 15, Issue 7 e04851 doi: 10.2903/j.efsa.2017.4851
Knutsen HK, Alexander J, Barregård L, Bignami M, Brüschweiler B, Ceccatelli S, Cottrill B, Dinovi M, Edler L, Grasl-Kraupp B, Hogstrand C....Zearalenone (ZEN), a mycotoxin primarily produced by Fusarium fungi, occurs predominantly in cereal grains. The European Commission asked EFSA for a scientific opinion on the risk to animal health related to ZEN and its modified forms in feed. Modified forms of ZEN occurring in feed include phase I metabolites α-zearalenol (α-ZEL), β-zearalenol (β-ZEL), α-zearalanol (α-ZAL), β-zearalanol (β-ZAL), zearalanone (ZAN) and phase II conjugates. ZEN has oestrogenic activity and the oestrogenic activity of the modified forms of ZEN differs considerably. For ZEN, the EFSA Panel on Contaminants...
Effect of linseed supplementation and slaughter age on meat quality of grazing cross-bred Galician x Burguete foals.
Journal of the science of food and agriculture    July 17, 2017   Volume 98, Issue 1 266-273 doi: 10.1002/jsfa.8466
Domínguez R, Pateiro M, Crecente S, Ruiz M, Sarriés MV, Lorenzo JM.The aim of this study was to assess the effect of finishing diet (control concentrate vs. linseed concentrate) and slaughter age (13 vs. 26 months) on meat and nutritional quality of foal meat. For this study, 46 foals from crossing Galicia Mountain x Burguete breeds were used. Results: The obtained results showed that slaughter age had an influence on chemical composition and colour parameters. Foals slaughtered at the age of 13 months had lower content of intramuscular fat and higher cholesterol contents than those slaughtered at 26 months of age. Regarding colour parameters, older foals sho...
Species-specific identification of collagen components in Colla corii asini using a nano-liquid chromatography tandem mass spectrometry proteomics approach.
International journal of nanomedicine    June 15, 2017   Volume 12 4443-4454 doi: 10.2147/IJN.S136819
Li X, Shi F, Gong L, Hang B, Li D, Chi L.Colla corii asini (CCA) is a protein-based traditional Chinese medicine made from donkey skins. Because it has the ability to nourish blood, its demand is increasing rapidly. The shortage of donkey skins increases the risk of the adulteration of CCA products with other animal skins. To ensure the drug efficacy and safety of CCA products, a proteomics technique was applied to reveal proteins in the skins of donkey, horse, cattle, and pig. Species-specific peptides for each animal species were predicted using bioinformatics, and their presence in the skins and gelatin samples was examined by nan...
[New drugs for horses and production animals in 2016].
Tierarztliche Praxis. Ausgabe G, Grosstiere/Nutztiere    May 17, 2017   Volume 45, Issue 3 176-181 doi: 10.15653/TPG-170267
Emmerich IU.In 2016, only one newly developed active pharmaceutical ingredient for horses and food-producing animals was released on the German market for veterinary drug products. The immunomodulator Pegbovigrastim is now available as an injection solution for cattle (Imrestor). Four established veterinary active pharmaceutical ingredients are available for further species: the ectoparasitic Amitraz (Apitraz) from the triazapentadiene group was additionally authorized for honeybees, the expectorant Bromhexine (Exflow Vet) for chickens, turkeys and ducks and the macrolide antibiotic Gamithromycin (Zactran...
Molecular confirmation of Sarcocystis fayeri in a donkey.
Veterinary parasitology    April 29, 2017   Volume 240 30-33 doi: 10.1016/j.vetpar.2017.04.026
Coultous RM, Raftery AG, Shiels BR, Sutton DGM, Weir W.Sarcocystis fayeri is a canine protozoan parasite with an equine intermediate host. Historically classified as an incidental pathogen, recent literature has described the toxic effects of Sarcocystis fayeri in human food poisoning, and highlighted potential involvement in equine neuromuscular disease. Until now, horses were believed to be the exclusive intermediate host. This study reports the first molecular confirmation of S. fayeri in a donkey, and gives rise to the consideration of donkeys being a potential reservoir for the parasite. This finding is of particular importance in understandi...
Science-in-brief: Clinical highlights from 50th Congress of the South African Equine Veterinary Association 2017.
Equine veterinary journal    April 8, 2017   Volume 49, Issue 3 266-268 doi: 10.1111/evj.12678
Miller SM, Spargo KE.No abstract available
[Doping relevant substances in horse feed].
Schweizer Archiv fur Tierheilkunde    April 7, 2017   Volume 159, Issue 4 231-235 doi: 10.17236/sat00112
Herholz C, Zink N, Laska H, Gumpendobler M, Trolliet C, Probst S.Horse feed material of Swiss and foreign production available on the Swiss market was tested with regard to possible contamination with 7 different alkaloids, atropine and colchicine. Twenty-eight feed samples as well as 2 poppy seed samples were analyzed. Out of 28 feed samples 18 were positive for prohibited substances in Equestrian sports. The concentration of prohibited substances was in none of the samples high enough to cause an effect on the body of the horse. The poppy seed sample, which was obtained by conventional harvesting and cleaning methods, contained a very high Morphine concen...
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