Analyze Diet

Topic:Kinematics

Kinematics in horses refers to the study of motion without considering the forces that cause it. This field involves analyzing the movement patterns of horses, including gait, stride length, joint angles, and limb trajectories. Kinematic analysis is performed using technologies such as high-speed cameras and motion capture systems to collect data on equine locomotion. This information is valuable for understanding normal and abnormal movement, assessing performance, and identifying potential lameness or injury. The study of kinematics provides insights into the biomechanics of horse movement and contributes to advancements in training, rehabilitation, and veterinary care. This page compiles peer-reviewed research studies and scholarly articles that explore the methodologies, findings, and applications of kinematic analysis in equine science.
Equine spinal kinematics derived from different riding positions during asymmetrical bareback riding.
Journal of equine science    September 6, 2021   Volume 32, Issue 3 81-89 doi: 10.1294/jes.32.81
Nuchprayoon N, Ritruechai P, Watchararat K, Limroongruengrat W, Wongtawan T, Arya N.Hippotherapy in patients with neuromuscular dysfunction creates high focal pressure on the pony's back due to bareback riding and an asymmetrical riding position. This study aimed to investigate the acute effect of asymmetrical bareback riding on the pony's spinal kinematics, blood lactate, serum creatine kinase, heart rate, and temperament score. Eight ponies were selected, and they were walked on a treadmill for 45 min on each experimental day, including warm-up (5 min), weight-loading by mannequin (30 min), and cool-down (10 min) sessions. During the weight-loading session, three different ...
Physical therapy treatments incorporating equine movement: a pilot study exploring interactions between children with cerebral palsy and the horse.
Journal of neuroengineering and rehabilitation    September 6, 2021   Volume 18, Issue 1 132 doi: 10.1186/s12984-021-00929-w
Lightsey P, Lee Y, Krenek N, Hur P.Physical therapy treatments incorporating equine movement are recognized as an effective tool to treat functional mobility and balance in children with cerebral palsy (CP). To date, only a few studies examined kinematic outputs of the horses and children when mounted. In this pilot study, to better understand the effectiveness of this type of treatment, we examined the interaction between the horses and children with CP during physical therapy sessions where equine movement was utilized. Four children with CP participated in eight physical therapy sessions incorporating hippotherapy as a treat...
The influence of equine limb conformation on the biomechanical responses of the hoof: An in vivo and finite element study.
Journal of biomechanics    August 28, 2021   Volume 128 110715 doi: 10.1016/j.jbiomech.2021.110715
Akbari Shahkhosravi N, C R Bellenzani M, M S Davies H, Komeili A.Hoof conformation plays a key role in equine locomotion. Toe-in conformation is an abnormal condition characterized by inward deviation of the limb from its frontal axis. Several studies have documented differences in hoof deformation and hoof kinematics in horses with toe-in and normal hoof conformations. However, the reason behind this has yet to be understood. The present study hypothesizes that a different center of pressure (COP) path underneath the hoof is the cause of different deformation patterns and hoof kinematics in toe-in hooves. In vivo measurements and finite element (FE) analys...
3D kinematic of the thoracolumbar spine in Mangalarga Marchador horses performing the marcha batida gait and being led by hand-A preliminary report.
PloS one    July 6, 2021   Volume 16, Issue 7 e0253697 doi: 10.1371/journal.pone.0253697
Simonato SP, Bernardina GRD, Ferreira LCR, Silvatti AP, Barcelos KMC, da Fonseca BPA.This study aimed to provide a preliminary description of the sagittal and transverse plane kinematics of the thoracolumbar spine of Mangalarga Marchador (MM) horses performing the marcha batida gait, led in-hand. We evaluated the pattern of angular movement and the mean amplitude of six specific angles. An optoelectronic system was used for 3D kinematic analysis (19 cameras, 250 Hz). They were positioned around the horses and an acquisition volume of 16 × 4.8 × 3 meters was used. Eight retroreflective markers were fixed on the spine of the animals over thoracic vertebrae 8 (T8), 12 (T12), 15...
Accelerometric Evaluation of the Locomotor Pattern After Administration of Morphine in Conscious Healthy Horses.
Journal of equine veterinary science    July 2, 2021   Volume 104 103701 doi: 10.1016/j.jevs.2021.103701
López-Sanromán FJ, Freilich GM, Gomez-Cisneros D, Varela M, Santiago I, Manso-Díaz G.The objective of the present study was to compare, using accelerometry, the gait changes produced after administration of a dose of 0.2 mg/kg of morphine at the walk in healthy horses. Six mature horses were used, and all animals received two different treatments with, at least, two weeks interval in between. Treatments administered consisted of a single dose of 10 ml of saline solution or a total of 0.2 mg/kg of morphine diluted in 10 ml of saline solution. A three-dimensional accelerometric device was used to collect data continuously while horses were walking. The walking test was performed...
Characteristics of the Flight Arc in Horses Jumping Three Different Types of Fences in Olympic Competition.
Journal of equine veterinary science    June 25, 2021   Volume 104 103698 doi: 10.1016/j.jevs.2021.103698
Clayton HM, St George L, Sinclair J, Hobbs SJ.Show jumping horses must execute fences of varying height and width, but the effect of this on jumping kinematics during the airborne phase have not been described. The aim of this study was to describe differences within- and between-horses in CM trajectory, trunk orientation and average trunk angular velocity in a group of elite horses executing three fences: vertical fence (1.60 m), spread fence (1.50 × 1.80 m), water jump (4.5 m) during an Olympic competition. Two-dimensional kinematic data (60 Hz) were collected from video cameras set perpendicular to each fence. After manual digitizat...
Continuous versus discrete data analysis for gait evaluation of horses with induced bilateral hindlimb lameness.
Equine veterinary journal    June 23, 2021   Volume 54, Issue 3 626-633 doi: 10.1111/evj.13451
Smit IH, Hernlund E, Brommer H, van Weeren PR, Rhodin M, Serra Bragança FM.Gait kinematics measured during equine gait analysis are typically evaluated by analysing (asymmetry-based) discrete variables (eg, peak values) obtained from continuous kinematic signals (eg, timeseries of datapoints). However, when used for the assessment of complex cases of lameness, such as bilateral lameness, discrete variable analysis might overlook relevant functional adaptations. Objective: The overall aim of this paper is to compare continuous and discrete data analysis techniques to evaluate kinematic gait adaptations to lameness. Methods: Method comparison. Methods: Sixteen healthy ...
Differences in equine spinal kinematics between straight line and circle in trot.
Scientific reports    June 18, 2021   Volume 11, Issue 1 12832 doi: 10.1038/s41598-021-92272-2
Byström A, Hardeman AM, Serra Bragança FM, Roepstorff L, Swagemakers JH, van Weeren PR, Egenvall A.Work on curved tracks, e.g. on circles, is commonplace within all forms of horse training. Horse movements in circles are naturally asymmetric, including the load distribution between inner and outer limbs. Within equestrian dressage the horse is expected to bend the back laterally to follow the circle, but this has never been studied scientifically. In the current study 12 horses were measured (optical motion capture, 100 Hz) trotting on left and right circles and on the straight without rider (soft surface). Data from markers placed along the spine indicated increased lateral bending to the...
Movement asymmetries in horses presented for prepurchase or lameness examination.
Equine veterinary journal    June 9, 2021   Volume 54, Issue 2 334-346 doi: 10.1111/evj.13453
Hardeman AM, Egenvall A, Serra Bragança FM, Koene MHW, Swagemakers JH, Roepstorff L, van Weeren R, Byström A.The increasing popularity of objective gait analysis makes application in prepurchase examinations (PPE) a logical next step. Therefore, there is a need to have more understanding of asymmetry during a PPE in horses described on clinical evaluation as subtly lame. Objective: The objective of this study is to objectively compare asymmetry in horses raising minor vet concerns in a PPE and in horses raising major vet concerns with that found in horses presented with subtle single-limb lameness, and to investigate the effect of age/discipline on the clinicians' interpretation of asymmetry on the c...
The Protraction and Retraction Angles of Horse Limbs: An Estimation during Trotting Using Inertial Sensors.
Sensors (Basel, Switzerland)    May 30, 2021   Volume 21, Issue 11 3792 doi: 10.3390/s21113792
Sapone M, Martin P, Ben Mansour K, Chateau H, Marin F.The protraction and retraction angles of horse limbs are important in the analysis of horse locomotion. This study explored two methods from an IMU positioned on the canon bone of eight horses to estimate these angles. Each method was based on a hypothesis in order to define the moment corresponding with the verticality of the canon bone: (i) the canon bone is in a vertical position at 50% of the stance phase or (ii) the verticality of the canon bone corresponds with the moment when the horse's withers reach their lowest point. The measurements were carried out on a treadmill at a trot and com...
Biplanar High-Speed Fluoroscopy of Pony Superficial Digital Flexor Tendon (SDFT)-An In Vivo Pilot Study.
Veterinary sciences    May 27, 2021   Volume 8, Issue 6 92 doi: 10.3390/vetsci8060092
Wagner FC, Gerlach K, Geiger SM, Gittel C, Böttcher P, Mülling CKW.The superficial digital flexor tendon (SDFT) is the most frequently injured structure of the musculoskeletal system in sport horses and a common cause for early retirement. This project's aim was to visualize and measure the strain of the sound, injured, and healing SDFTs in a pony during walk and trot. For this purpose, biplanar high-speed fluoroscopic kinematography (FluoKin), as a high precision X-ray movement analysis tool, was used for the first time in vivo with equine tendons. The strain in the metacarpal region of the sound SDFT was 2.86% during walk and 6.78% during trot. When injured...
A New Approach of Sperm Motility Subpopulation Structure in Donkey and Horse.
Frontiers in veterinary science    May 25, 2021   Volume 8 651477 doi: 10.3389/fvets.2021.651477
Gacem S, Valverde A, Catalán J, Yánez Ortiz I, Soler C, Miró J.This study aimed to characterize the sperm kinematic values with high frames per second, to define the subpopulation structure of a horse and a donkey and compare them. A total of 57 fresh semen ejaculates (26 Spanish and 16 Arabian horse breeds and 10 donkeys) were collected and subsequently analyzed for kinematic parameters using the Computer-aided sperm motility analysis ISAS®v1.2 system and using a Spermtrack® 10-μm depth counting chamber. Sequences were recorded at 250 frames per second, and eight kinematic parameters were automatically evaluated. All kinematic parameters showed signif...
Development of An Anybody Musculoskeletal Model of The Thoroughbred Forelimb.
Journal of equine veterinary science    May 24, 2021   Volume 103 103666 doi: 10.1016/j.jevs.2021.103666
Bardin AL, Tang L, Panizzi L, Rogers CW, Colborne GR.Musculoskeletal injuries in horses are the main cause of retirement, rest, and death. To understand these injuries, it is necessary to study loads in muscles, tendons and ligaments. A musculoskeletal model makes it possible to consider all structures simultaneously and avoids invasive measurements. At present, most computational models of the equine limb described in the literature have been limited to the distal limb. The aim of this study was to create a preliminary musculoskeletal model of the whole equine forelimb and to run it with kinematic data collected during gait. The model was devel...
Differential rotational movement and symmetry values of the thoracolumbosacral region in high-level dressage horses when trotting.
PloS one    May 6, 2021   Volume 16, Issue 5 e0251144 doi: 10.1371/journal.pone.0251144
MacKechnie-Guire R, Pfau T.High-level dressage horses regularly perform advanced movements, requiring coordination and force transmission between front and hind limbs across the thoracolumbosacral region. This study aimed at quantifying kinematic differences in dressage horses when ridden in sitting trot-i.e. with additional load applied in the thoracolumbar region-compared with trotting in-hand. Inertial sensors were glued on to the midline of the thoracic (T) and lumbar (L) spine at T5, T13, T18, L3 and middle of the left and right tubera sacrale of ten elite dressage horses (Mean±SD), age 11±1 years, height 1.70±0...
Detection of Equine Hoof Motion by Using a Hoof-Mounted Inertial Measurement Unit Sensor in Comparison to Examinations with an Optoelectronic Technique – A Pilot Study.
Journal of equine veterinary science    March 26, 2021   Volume 101 103454 doi: 10.1016/j.jevs.2021.103454
Hagen J, Jung FT, Brouwer J, Bos R.This study aimed to determine whether a hoof-mounted inertial measurement unit (IMU) system would provide similar timing characteristics and step lengths, in walk and trot on different grounds, compared to those of an established optoelectronic motion system. The right hoof of one horse was equipped with an IMU sensor with an optical reference marker on top. Fifteen steps on firm ground, and five on penetrable ground, were performed at walk and trot. Level of agreement between the two measurement systems was determined by assessing the concordance correlation coefficients, accuracy, and precis...
Assessment of the effect of horseshoes with and without traction adaptations on the gait kinetics of nonlame horses during a trot on a concrete runway.
American journal of veterinary research    March 26, 2021   Volume 82, Issue 4 292-301 doi: 10.2460/ajvr.82.4.292
Wang P, Takawira C, Taguchi T, Niu X, Nazzal MD, Lopez MJ.To assess the effect of horseshoes with and without traction adaptations on the gait kinetics of nonlame horses during a trot on a concrete runway. Methods: 5 nonlame adult light-breed horses. Methods: Kinetic data were obtained for each horse when it was trotted across a force platform within a concrete runway unshod (control) and shod with standard horseshoes; standard horseshoes with high profile-low surface area calks, with low profile-high surface area calks, and coated with a thin layer of tungsten carbide (TLTC); and plastic-steel composite (PSC) horseshoes. Kinetic data were obtained f...
Differential Rotational Movement of the Thoracolumbosacral Spine in High-Level Dressage Horses Ridden in a Straight Line, in Sitting Trot and Seated Canter Compared to In-Hand Trot.
Animals : an open access journal from MDPI    March 20, 2021   Volume 11, Issue 3 888 doi: 10.3390/ani11030888
MacKechnie-Guire R, Pfau T.Assessing back dysfunction is a key part of the investigative process of "loss of athletic performance" in the horse and quantitative data may help veterinary decision making. Ranges of motion of differential translational and rotational movement between adjacent inertial measurement units attached to the skin over thoracic vertebrae 5, 13 and 18 (T5, T13, T18) lumbar vertebra 3 (L3) and tuber sacrale (TS) were measured in 10 dressage horses during trot in-hand and ridden in sitting trot/canter. Straight-line motion cycles were analysed using a general linear model (random factor: horse; fixed...
Inter-evaluator and Intra-evaluator Reliability of a Software Program Used to Extract Kinematic Variables Obtained by an Extremity-Mounted Inertial Measurement Unit System in Sound Horses at the Trot Under Soft and Hard Ground Conditions and Treadmill Exercise.
Frontiers in veterinary science    March 4, 2021   Volume 8 595455 doi: 10.3389/fvets.2021.595455
Schwarz J, Vidondo B, Maninchedda UE, Sprick M, Schöpfer MC, Cruz AM. To assess the inter-evaluator and intra-evaluator reliability of a software program used to extract kinematic variables by a commercially available extremity-mounted inertial measurement unit system in sound horses at the trot under soft and hard ground conditions and treadmill exercise. Thirty adult, sound and healthy French Montagne stallions. Data collection was performed with six IMUs strapped to the distal, metacarpal, metatarsal and tibial regions of every horse. Per surface (treadmill, soft and hard ground) 10 stallions were trotted three times. Prior to the analysis done by six eval...
In vitro assessment of the motion of equine proximal sesamoid bones relative to the third metacarpal bone under physiologic midstance loads.
American journal of veterinary research    February 26, 2021   Volume 82, Issue 3 198-206 doi: 10.2460/ajvr.82.3.198
Shaffer SK, Sachs N, Garcia TC, Fyhrie DP, Stover SM.To assess the motion of the proximal sesamoid bones (PSBs) relative to the third metacarpal bone (MC3) of equine forelimbs during physiologic midstance loads. Methods: 8 musculoskeletally normal forelimbs (7 right and 1 left) from 8 adult equine cadavers. Methods: Each forelimb was harvested at the mid-radius level and mounted in a material testing system so the hoof could be moved in a dorsal direction while the radius and MC3 remained vertical. The PSBs were instrumented with 2 linear variable differential transformers to record movement between the 2 bones. The limb was sequentially loaded ...
Construction of a Realistic, Whole-Body, Three-Dimensional Equine Skeletal Model using Computed Tomography Data.
Journal of visualized experiments : JoVE    February 25, 2021   Issue 168 doi: 10.3791/62276
Lee AKK, Uhl EW, Osborn ML.Therapies based upon whole-body biomechanical assessments are successful for injury prevention and rehabilitation in human athletes. Similar approaches have rarely been used to study equine athletic injury. Degenerative osteoarthritis caused by mechanical stress can originate from chronic postural dysfunction, which, because the primary dysfunction is often distant from the site of tissue injury, is best identified through modeling whole-body biomechanics. To characterize whole-body equine kinematics, a realistic skeletal model of a horse was created from equine computed tomography (CT) data t...
Effect of bioactive proteins on gait kinematics and systemic inflammatory markers in mature horses.
Translational animal science    February 8, 2021   Volume 5, Issue 1 txab017 doi: 10.1093/tas/txab017
Fikes KK, Coverdale JA, Leatherwood JL, Campbell JM, Welsh TH, Hartz CJ, Goehring M, Millican AA, Bradbery AN, Wickersham TA.Twenty-seven mature Quarter horses were used in a randomized design to determine the effects of bioactive protein supplementation on gait kinematics and systemic inflammatory markers in a 34-d trial. Treatments consisted of oral doses of 230 g/d of pelleted supplements containing 0 g (CON; = 9), 40 g of bioactive protein (40BP; = 9; LIFELINE, APC, LLC, Ankeny, IA), and 80 g of bioactive protein (80BP; = 9) daily. Horses were fed a commercial concentrate at 0.5% BW (as-fed) and received coastal bermudagrass () hay daily. On day 33, horses consistent in exercise (CON, = 6; 40BP, = 8; 80BP,...
Muscle Function and Kinematics during Submaximal Equine Jumping: What Can Objective Outcomes Tell Us about Athletic Performance Indicators?
Animals : an open access journal from MDPI    February 5, 2021   Volume 11, Issue 2 414 doi: 10.3390/ani11020414
St George L, Clayton HM, Sinclair J, Richards J, Roy SH, Hobbs SJ.Selection and training practices for jumping horses have not yet been validated using objective performance analyses. This study aimed to quantify the differences and relationships between movement and muscle activation strategies in horses with varying jump technique to identify objective jumping performance indicators. Surface electromyography (sEMG) and three-dimensional kinematic data were collected from horses executing a submaximal jump. Kinematic variables were calculated based on equestrian-derived performance indicators relating to impulsion, engagement and joint articulation. Horses ...
Effects of sand, asphalt and 3-degree hind toe or heel elevation on horse kinematics.
The Veterinary record    January 24, 2021   Volume 188, Issue 4 e23 doi: 10.1002/vetr.23
Caure S, Bonomelli N, Carro M, Leveillard D, Blanville F, Mortagne P, Cousty M, Weller R.Although the effects of both the surfaces and plantar angles on equine locomotion have been widely discussed, limited scientific data are available. Methods: Our objectives were to determine the effects of two surfaces (asphalt and sand) and of 3-degree hind toe or heel elevation on horse kinematics in an experimental study. Six saddle horses were shod with a reference shoeing (REF), characterized by a fore aluminium (REF F) and hind steel racehorse (REF H) shoeing. Two dimensional kinematic videos compared horse's kinematic parameters when walking and trotting on asphalt and sand. On asphalt,...
Breath-Jockey: Development and Feasibility Assessment of a Wearable System for Respiratory Rate and Kinematic Parameter Estimation for Gallop Athletes.
Sensors (Basel, Switzerland)    December 29, 2020   Volume 21, Issue 1 152 doi: 10.3390/s21010152
Di Tocco J, Sabbadini R, Raiano L, Fani F, Ripani S, Schena E, Formica D, Massaroni C.In recent years, wearable devices for physiological parameter monitoring in sports and physical activities have been gaining momentum. In particular, some studies have focused their attention on using available commercial monitoring systems mainly on horses during training sessions or competitions. Only a few studies have focused on the jockey's physiological and kinematic parameters. Although at a glance, it seems jockeys do not make a lot of effort during riding, it is quite the opposite. Indeed, especially during competitions, they profuse a short but high intensity effort. To this extend, ...
The effect of curve running on distal limb kinematics in the Thoroughbred racehorse.
PloS one    December 29, 2020   Volume 15, Issue 12 e0244105 doi: 10.1371/journal.pone.0244105
Parkes RSV, Pfau T, Weller R, Witte TH.During racing, injury is more likely to occur on a bend than on a straight segment of track. This study aimed to quantify the effects of galloping at training speeds on large radius curves on stride parameters and limb lean angle in order to assess estimated consequences for limb loading. Seven Thoroughbred horses were equipped with a sacrum-mounted inertial measurement unit with an integrated GPS, two hoof-mounted accelerometers and retro-reflective markers on the forelimbs. Horses galloped 2-4 circuits anticlockwise around an oval track and were filmed at 120 frames per second using an array...
Kinematic and kinetic analyses of the gait of horses wearing novel legwear for variably limiting extension of the metacarpophalangeal joint.
American journal of veterinary research    December 29, 2020   Volume 82, Issue 1 48-54 doi: 10.2460/ajvr.82.1.48
St George LB, Pugliese BR, Hobbs SJ, Brisbois AL, Sinclair JK, Kirker-Head CA.To investigate the effects of novel legwear designed to limit metacarpophalangeal joint (MCPJ) extension and redirect loading forces from the flexor apparatus through analyses of 2-D kinematic and kinetic data. Methods: 6 adult horses without musculoskeletal disease. Methods: Horses were subjected to 4 treatments: control (no legwear), inactive legwear (unlimited legwear extension), and active legwear with mild (30°) and moderate (20°) legwear extension limitation. Two-dimensional kinematic data were collected for the right forelimb (FL) during walk and trot and from leading and trailing FLs...
Jockey Perception of Shoe and Surface Effects on Hoof-Ground Interactions and Implications for Safety in the Galloping Thoroughbred Racehorse.
Journal of equine veterinary science    November 21, 2020   Volume 97 103327 doi: 10.1016/j.jevs.2020.103327
Horan K, Kourdache K, Coburn J, Day P, Brinkley L, Carnall H, Harborne D, Hammond L, Millard S, Pfau T.Riding racehorses is a high-risk profession and optimizing safety alongside performance is paramount. Horseshoes play a critical role in providing traction with the ground surface and are therefore a major determinant of safety. However, the subjective perceptions of expert riders influence attitudes towards using different shoes and must be taken into consideration before any changes may be implemented. This study used a questionnaire-based method to evaluate jockey opinion of four shoeing conditions (aluminum, steel, GluShu, and barefoot) trialed at gallop over turf and artificial surfaces. ...
Reliable and clinically applicable gait event classification using upper body motion in walking and trotting horses.
Journal of biomechanics    November 21, 2020   Volume 114 110146 doi: 10.1016/j.jbiomech.2020.110146
Roepstorff C, Dittmann MT, Arpagaus S, Serra Bragança FM, Hardeman A, Persson-Sjödin E, Roepstorff L, Gmel AI, Weishaupt MA.Objectively assessing horse movement symmetry as an adjunctive to the routine lameness evaluation is on the rise with several commercially available systems on the market. Prerequisites for quantifying such symmetries include knowledge of the gait and gait events, such as hoof to ground contact patterns over consecutive strides. Extracting this information in a robust and reliable way is essential to accurately calculate many kinematic variables commonly used in the field. In this study, optical motion capture was used to measure 222 horses of various breeds, performing a total of 82 664 steps...
Kinematic effects of the circle with and without rider in walking horses.
PeerJ    November 18, 2020   Volume 8 e10354 doi: 10.7717/peerj.10354
Egenvall A, Engström H, Byström A.Biomechanical studies of walk, especially walk on the circle, are scarce, while circles or curved tracks are frequently used during equestrian activities. To study horse-rider-circle interactions on the circle, the first steps would be to investigate how the unridden, freely walking horse is influenced by circular movement, and then add a rider. The aim was to study horse vertical trunk movements, and sagittal cannon angles (protraction-retraction) during walk in straight-line and on the circle without rider, and on the circle with a rider using minimal influence. Methods: Ten horses were ridd...
Optimal frame rate when there were stallion sperm motility evaluations and determinations for kinematic variables using CASA-Mot analysis in different counting chambers.
Animal reproduction science    November 2, 2020   Volume 223 106643 doi: 10.1016/j.anireprosci.2020.106643
Gacem S, Bompart D, Valverde A, Catalán J, Miró J, Soler C.This study was conducted to determine optimum image capture frame rates (FRO) when there was evaluation of different types of counting chambers used for CASA-Mot determinations of stallion sperm motility. Sperm VCL was determined at frame rates of 25-250 f/s in: 1) Spermtrack® (Spk) 10 and 20 chambers (drop displacement-type chambers 10 and 20 μm-deep respectively; and 2) ISAS®D4C10, ISAS®D4C20 (10 and 20 μm-deep respectively) and ISAS®D4C20 L (20 μm-deep) capillary loaded chambers. Values for different sperm kinematic variables were determined using each chamber at 250 f/s, whi...