A longitudinal study in the context of equine research involves the systematic observation and data collection from the same group of horses over an extended period. This research approach aims to track changes and developments in various health parameters, behaviors, or conditions within the equine population being studied. By repeatedly assessing the same subjects, researchers can identify patterns, establish temporal sequences, and infer potential causal relationships. Longitudinal studies in horses can encompass a wide range of topics, including growth and development, disease progression, and the impact of environmental or management changes. This page gathers peer-reviewed research studies and scholarly articles that explore the methodologies, findings, and implications of longitudinal studies in equine science.
Rohrbach BW, Stafford JR, Clermont RS, Reed SM, Schott HC, Andrews FM.Pituitary pars intermedia dysfunction (PPID) is common in older horses. Objective: To determine diagnosis frequency, prognostic factors, long-term survival, and owner satisfaction with treatment. Methods: Medical records from horses diagnosed with PPID, 1993-2004. Methods: A retrospective cohort design with data collected from the Veterinary Medical Data Base (VMDB) and a cohort of 3 VTHs. Proportional accessions, annual incidence, and demographics were compared for all accessions. During the same period, a subset of medical records (n = 44) was extracted and owners (n = 34) contacted to obtai...
Johns I, Verheyen K, Good L, Rycroft A.The objective of this study was to examine the emergence and persistence of antimicrobial resistant faecal Escherichia coli in horses treated with antimicrobial drugs in a hospital and community setting. Faecal samples were collected from hospitalised (n=56) and non-hospitalised (n=14) horses treated with antimicrobials, and 10 non-treated hospitalised controls. Samples were obtained pre-treatment and 5 days later in all horses, and 2 weeks and 2 months after treatment in treated horses. Susceptibility to 15 antimicrobials was tested via disc diffusion on up to 3 E. coli isolates per sample. P...
Schoster A, Staempfli HR, Arroyo LG, Reid-Smith RJ, Janecko N, Shewen PE, Weese JS.Point prevalence studies have reported carriage rates of enteric pathogens in healthy horses, but longitudinal data are lacking. Commensal E. coli is an indicator organism to evaluate antimicrobial resistance of enteric bacteria, yet there are limited data for horses. The objectives of this study were to investigate and molecularly characterize isolates of Clostridium difficile, Clostridium perfringens and Salmonella, collected sequentially over a one year period, and to determine the antibiotic susceptibility profile for E. coli. Fecal samples were collected monthly from 25 adult horses for o...
Buckley P, Morton JM, Buckley DJ, Coleman GT.Horse misbehaviour is an important cause of poor performance in Pony Club horses, is associated with horse-related rider injuries and has been implicated as a nonspecific presenting sign for musculoskeletal pain. Despite this, little is known about the incidence of and risk factors for misbehaviour in Pony Club horses. Objective: This study aimed to describe the incidence and types of misbehaviour in a cohort of Pony Club horses and to identify risk factors for misbehaviour during riding. Methods: A prospective longitudinal study was conducted with 84 Pony Club horses from 41 families belongin...
Tanner JC, Rogers CW, Firth EC.There is increasing evidence that exercise early in life has a positive effect on musculoskeletal health. At present, there is little whole population research investigating the effect of racing as 2-year-olds on future racing career. Objective: To investigate the association between attaining training milestones as 2-year-olds with length of career and racing success in Thoroughbred horses in New Zealand. Methods: Retrospective data were obtained of the 2001/02-born Thoroughbred foal crop. The 3 training milestones were: registered with a trainer, trialled and raced. The association of the tr...
Cavens VJ, Gemensky-Metzler AJ, Wilkie DA, Weisbrode SE, Lehman AM.To describe the long-term histologic and intraocular pressure (IOP) lowering effects of diode laser transscleral cyclophotocoagulation (TSCP) on the normal equine eye. Methods: Eight normal adult horses. Methods: TSCP was performed in one randomly assigned eye. Sixty spots were treated at settings of 1500 ms and 1500 mW. Two horses were randomly selected for euthanasia at 2, 4, 12, or 24 weeks post-TSCP. Both eyes were enucleated and histologically evaluated. Intraocular pressure was measured by applanation tonometry prior to TSCP, immediately post-TSCP, twice daily for 7 days post-TSCP and th...
Buhl R, Ersbøll AK.To assess whether physical training induces cardiac hypertrophy and valvular regurgitation in maturing Standardbred trotters and to establish a prediction model for the size of the left ventricle. Methods: Longitudinal observational study. Methods: 53 Standardbred trotters. Methods: Each horse underwent 2-D, M-mode, and color flow Doppler echocardiography at 5.5 years of age; previously, each horse had been examined at 2, 2.5, 3, and 3.5 (time of maturity) years of age. Horses were or were not in training or racing for variable periods during the entire assessment period; data for a given hors...
Kern JK, Fletcher CL, Garver CR, Mehta JA, Grannemann BD, Knox KR, Richardson TA, Trivedi MH.Anecdotal reports and some studies suggest that equine-assisted activities may be beneficial in autism spectrum disorders (ASD). Objective: To examine the effects ofequine-assisted activities on overall severity of autism symptoms using the Childhood Autism Rating Scale (CARS) and the quality ofparent-child interactions using the Timberlawn Parent-Child Interaction Scale. In addition, this study examined changes in sensory processing, quality of life, and parental treatment satisfaction. Methods: Children with ASD were evaluated at four time points: (1) before beginning a 3-to-6 month waiting ...
Field H, de Jong C, Melville D, Smith C, Smith I, Broos A, Kung YH, McLaughlin A, Zeddeman A.Hendra virus is a recently emerged zoonotic agent in Australia. Since first described in 1994, the virus has spilled from its wildlife reservoir (pteropid fruit bats, or 'flying foxes') on multiple occasions causing equine and human fatalities. We undertook a three-year longitudinal study to detect virus in the urine of free-living flying foxes (a putative route of excretion) to investigate Hendra virus infection dynamics. Pooled urine samples collected off plastic sheets placed beneath roosting flying foxes were screened for Hendra virus genome by quantitative RT-PCR, using a set of primers a...
Bisanzio D, Giacobini M, Bertolotti L, Mosca A, Balbo L, Kitron U, Vazquez-Prokopec GM.West Nile Virus (WNV) transmission in Italy was first reported in 1998 as an equine outbreak near the swamps of Padule di Fucecchio, Tuscany. No other cases were identified during the following decade until 2008, when horse and human outbreaks were reported in Emilia Romagna, North Italy. Since then, WNV outbreaks have occurred annually, spreading from their initial northern foci throughout the country. Following the outbreak in 1998 the Italian public health authority defined a surveillance plan to detect WNV circulation in birds, horses and mosquitoes. By applying spatial statistical analysi...
Christophersen MT, Tnibar A, Pihl TH, Andersen PH, Ekstrøm CT.There is a paucity of studies addressing sporting activity and horse owners' satisfaction after horses have undergone colic surgery. Objective: To determine 1) survival rate after colic surgery, 2) prevalence of horses returning to, or starting, sporting activities and 3) assess the owners' satisfaction regarding colic surgery. Methods: Cases that underwent exploratory celiotomy for colic between January 2005 and August 2010 were reviewed. All horses that had one or more celiotomies and were discharged after colic surgery were included in a telephone questionnaire survey. Only horses that surv...
Read AJ, Arzey KE, Finlaison DS, Gu X, Davis RJ, Ritchie L, Kirkland PD.An outbreak of equine influenza (EI) occurred in Australia in 2007. During the laboratory support for this outbreak, real-time reverse transcriptase polymerase chain reaction (qRT-PCR) assays and a blocking enzyme linked immunosorbent assay (bELISA) were used as testing methods to detect infection with the virus. The qRT-PCR and bELISA tests had not been used for EI diagnosis before, so it was not known how soon after infection these tests would yield positive results, or for how long these results would remain positive. To answer these questions, nasal swabs and blood samples were collected d...
Knight PK, Thomson PC.OBJECTIVE Compare the career profiles of a cohort of Standardbred horses that first raced as 2-year-olds with those that started their racing careers at a later age. METHOD Retrospective analysis of the racing records of all foals born in New South Wales in the 2000 foaling season. RESULTS The career records of 999 horses were analysed. Almost half (43.9%) first raced as 2-year-olds and one-third (33.9%) as 3-year-olds. The median career duration for horses that first raced as 2-year-olds was 2.93 years (interquartile range (IQR) 2.70-3.16), which was significantly greater than the median for ...
Dyson S, Murray R.Neurectomy of the deep branch of the lateral plantar nerve and plantar fasciotomy have become accepted as methods of treatment of proximal suspensory desmopathy (PSD), but there are limited long-term studies documenting the outcome. Objective: To describe long-term follow-up in horses with PSD alone or with other injuries contributing to lameness and poor performance, including complications, following neurectomy and fasciotomy. Methods: Follow-up information was acquired for 155 horses that had undergone neurectomy and fasciotomy for treatment of PSD between 2003 and 2008. Success was classif...
Whitfield-Cargile CM, Rakestraw PC, Hardy J, Cohen ND, Davis BE.Repair of incisional hernias in horses has been described previously; however, this report describes the outcome of primary closure of incisional hernias in a large number of horses and compares these results with those of mesh implantation. Objective: To report the perioperative care, complications and long-term outcome of primary closure of incisional hernias in horses and to compare these results with a second population of horses in which prosthetic mesh was used. Methods: Medical records of horses undergoing an incisional herniorrhaphy between 1998 and 2009 were reviewed. Information obta...
Dixon PM, Parkin TD, Collins N, Hawkes C, Townsend N, Tremaine WH, Fisher G, Ealey R, Barakzai SZ.There is limited objective information available on the treatment and the long-term response to treatment of the different types of equine sinus disease. Objective: To document the treatments and long-term response to these treatments in 200 cases of equine sinus disease (1997-2009). Methods: The treatments of horses affected with subacute primary sinusitis (n = 52); chronic primary sinusitis (n = 37); dental sinusitis (n = 40); sinus cyst (n = 26); traumatic (n = 13); dental-related oromaxillary fistula (n = 8); sinus neoplasia (n = 10); mycotic sinus disease (n = 7); and intrasinus progressi...
Scantlebury CE, Archer DC, Proudman CJ, Pinchbeck GL.The frequency of recurrent colic in the UK equine general practice population is previously unreported. Elucidating risk factors for recurrent colic could provide a basis for clinical decision making and interventions. Objective: To determine the incidence rate of and risk factors for recurrent colic. Objective: Horse management, prophylactic health care and innate behaviour contribute to the risk of recurrent colic. Methods: A cohort of 127 horses was enrolled at the point of a veterinary-diagnosed medical colic episode. Participating owners completed a baseline and 3 follow-up telephone ques...
Morgan R, Dyson S.Previous descriptions of incomplete longitudinal fractures and fatigue injury of the proximopalmar aspect of the third metacarpal bone (McIII) have focused on diagnostic imaging findings, especially in racehorses. Objective: To document the case details, clinical features, response to diagnostic analgesia, diagnostic imaging findings and follow-up data in a large group of horses with an incomplete longitudinal fracture or fatigue injury of the proximopalmar medial aspect of the McIII. Methods: Horses were included in the study if pain was localised to the proximopalmar aspect of the metacarpal...
Dias DP, Teixeira LG, Canola PA, Albernaz RM, Marques JA, Neto JC.The purpose of this investigation was to evaluate surgical catheter implantation in the facial artery of horses and the long-term maintenance of such arteries using heparin and ascorbic acid as filling solution. Nine horses were implanted with a polyurethane catheter. The catheters were flushed with a heparin/ascorbic acid solution every 8h and remained patent for 25 days. Arterial blood samples were collected twice a day, and one exercise test that included serial blood samples and arterial pressure recordings was performed on a treadmill. Polyurethane catheters surgically implanted in the fa...
Damborg P, Marskar P, Baptiste KE, Guardabassi L.The objective of this longitudinal study was to investigate the occurrence and genetic background of faecal Escherichia coli resistant to cefotaxime (CTX) in horses receiving broad-spectrum antimicrobial prophylaxis after admission to a veterinary teaching hospital. The ten horses enrolled in the study were treated with cefquinome either alone (n=4) or in combination with metronidazole (n=3) or other antimicrobial agents (n=3). CTX-resistant coliforms in faeces collected before, during and after treatment were quantified on selective MacConkey agar supplemented with CTX, and a colony isolated ...
Faehrmann P, Riddell K, Read AJ.We describe the clinical signs and disease course during an outbreak of equine influenza (EI) in naïve horses in a police stables in Sydney, New South Wales, Australia.
Read AJ, Finlaison DS, Gu X, Davis RJ, Arzey KE, Kirkland PD.During the equine influenza (EI) outbreak, two assays were used in parallel to diagnose the disease, to demonstrate freedom from infection in disease control zones and ultimately to demonstrate that EI virus had been eliminated from the Australian horse population. A longitudinal study of a population of naturally infected horses was established to determine the performance characteristics of these assays.
Walmsley EA, Anderson GA, Adkins AR.Determine the long-term outcome for Thoroughbreds undergoing desmotomy of the accessory ligament of the deep digital flexor tendon (DAL-DDFT) for type 1 flexural deformity of the distal interphalangeal joint (DIPJ). Methods: Retrospective matched cohort study Methods: Medical records were retrieved over a 17-year period for Thoroughbreds that underwent DAL-DDFT for type 1 flexural deformity. Long-term outcome was determined by analysis of race records and comparison with maternal siblings. Comparisons between cases and controls included 2-year-old, 3-year-old and total career performance data....
Rijkenhuizen AB, de Graaf K, Hak A, Fürst A, ter Braake F, Stanek C, Greet TR.A multicentre study of 285 cases was performed to enhance the management of distal phalangeal fractures on the basis of clinical evidence. The outcome after treatment was available for 223 of the cases. Horses with a non-articular type I fracture had a better prognosis (91.7%) for return to original or expected level of use than horses with an articular type II or III fracture (69.6% and 74.1%, respectively). The prognosis for types IV and V fractures was fair (57.7% and 57.1%, respectively) and for type VI good (80%). Horses with a hindlimb fracture had a significantly greater chance of a suc...
Sparks HD, Nixon AJ, Fortier LA, Mohammed HO.Long-term efficacy of arthroscopic cartilage reattachment for the treatment of osteochondritis dissecans (OCD) lesions in the equine femoropatellar joint is unknown. Objective: To evaluate radiographic outcome and long-term performance of horses undergoing OCD reattachment. Objective: Separated OCD cartilage flaps may be reincorporated into the joint surface by reattachment rather than flap removal. Methods: Polydioxanone pins were utilised arthroscopically to reattach OCD lesions in 40 of 44 joints from 27 horses. Cartilage was reattached when it had persisting perimeter continuity, the surfa...
Arensburg L, Wilderjans H, Simon O, Dewulf J, Boussauw B.Longitudinal tears (LTs) of the digital flexor tendons are an important cause of chronic tenosynovitis of the digital flexor tendon sheath (DFTS). The origin of those marginal tears is not yet fully understood. The long-term outcome after medical and surgical treatment is guarded. Objective: To determine the prevalence of LTs of the digital flexor tendons in a large population of horses undergoing diagnostic tenoscopy of the DFTS and to assess the outcome of surgical treatment and the factors influencing the outcome. Methods: Medical records of 130 horses with chronic tenosynovitis of the DFTS...
Jean D, Vrins A, Beauchamp G, Lavoie JP.To determine variations in cytologic counts of bronchoalveolar lavage (BAL) fluid attributable to month of collection, first and second aliquots, and left and right lung sites in horses with recurrent airway obstruction (RAO). Methods: 5 horses with RAO and 5 healthy horses without respiratory tract disease. Methods: Horses were housed in a stable for 5 months prior to and throughout the study. Bronchoalveolar lavage fluid was collected from the right and left lung of each horse 3 times at monthly intervals (February, March, and April). Each BAL fluid collection was performed by use of 2 incre...
De Graaf-Roelfsema E, Keizer HA, Wijnberg ID, Van Der Kolk JH.Gastric ulceration can be caused by different pathophysiological mechanisms including dietary factors, psychological stress and exercise. Overtraining is a medical syndrome in performance horses associated with altered hormone levels, altered feed intake, altered behaviour and decreased performance. These components might lead to a higher incidence of gastric ulceration in overtrained horses. Objective: To investigate whether the incidence of gastric ulceration is increased in overtrained compared to control horses. Methods: A longitudinal training study with twelve 1.5 years old Standardbred ...
Goachet AG, Varloud M, Philippeau C, Julliand V.In endurance horses, commonly fed 80% forage, energy is provided mainly by VFA produced in the hindgut during cell wall degradation, but cell wall digestibility has been reported to be impaired by exercise. Objective: To assess the influence of a long-term endurance conditioning on cell wall digestion in horses. Methods: The total tract apparent digestibility of dry matter, organic matter and fibrous constituents, solid and liquid total mean retention time and the faecal microbial ecosystem of purebred Arabian horses were measured for 2 years in 2 longitudinal experiments. Methods: Performed a...
Sloet van Oldruitenborgh-Oosterbaan MM, Genzel W, van Weeren PR.Welfare concerns over the reasons for interruption or termination of a sporting career in horses have been expressed. Objective: To study the career of competition horses and factors that influence career length. Methods: In 2004, 46,576 rider-horse combinations were registered with the Dutch National Equestrian Federation (KNHS) in dressage, show jumping, eventing and endurance. From this population, approximately 1% of horses in each discipline were selected at random and all recorded competition data from the KNHS registration system collected and supplemented by detailed rider/owner teleph...
Gehlen H, Schlaga A.Several studies have shown the effect of training on myocardial deformation parameters in human athletes. The aim of this prospective, longitudinal study was echocardiographic evaluation of myocardial velocities and deformation in horses during the first year of training. Twelve Standardbred yearlings were examined using tissue Doppler imaging (TDI) and two-dimensional speckle tracking (2D-ST) during the first year of race training (short axis of left and right ventricle). The first examination was performed before the beginning of training (n = 12). At the last time point, horses were fully ...
Rushton JO, Kolodziejek J, Tichy A, Nowotny N, Nell B.To date the influence of herpesviruses on the development of equine ocular diseases has not been clearly determined. Objective: The purpose of this study was to illustrate the course of equine ocular findings over a period of 18 months at 6 month intervals, in correlation with the results of herpesvirus detection. Methods: 266 Lipizzaners in 3 federal states of Austria underwent complete ophthalmologic examination 4 times. Blood samples, nasal- and conjunctival swabs were obtained at the same time and used for the detection of the equid gammaherpesviruses EHV-2 and EHV-5 using consensus herpes...
Pollaris E, Broeckx BJG, Vlaminck L. It has been suggested that fissures of the occlusal surface of equine cheek teeth may develop into crown fractures. To examine the evolution of fissures present on the occlusal surface of cheek teeth. Furthermore, to investigate the presence of a fissure as a risk factor for the development of a subsequent crown fracture. Observational longitudinal study. Bi-annual dental examinations were performed on 36 horses for 3 years. Video-recordings were made to evaluate the evolution of detected fissures. The effect of possible predictors on the development of tooth fractures was investigated by ...
Whitfield-Cargile CM, Rakestraw PC, Hardy J, Cohen ND, Davis BE.Repair of incisional hernias in horses has been described previously; however, this report describes the outcome of primary closure of incisional hernias in a large number of horses and compares these results with those of mesh implantation. Objective: To report the perioperative care, complications and long-term outcome of primary closure of incisional hernias in horses and to compare these results with a second population of horses in which prosthetic mesh was used. Methods: Medical records of horses undergoing an incisional herniorrhaphy between 1998 and 2009 were reviewed. Information obta...
Hardy J, Marcoux M, Breton L.Radiographs of all 4 fetlocks of 71 Standardbred racehorses were obtained at 3-month intervals for 1 year. Radiographic findings in the abaxial surface of the proximal sesamoid bones were classified into 3 types according to the severity of lesions, and correlation was made with clinical findings at time of examination. Type-1 lesions (1 or 2 linear defects less than or equal to 1 mm wide) were detected in 55% of horses at the start of training; clinical signs of disease were not manifested, and lesions did not become clinically relevant. Type-2 lesions (3 or more linear defects less than or e...
Carstanjen B, Amory H, Sulon J, Hars O, Remy B, Langlois P, Lepage OM.Bone responds to exercise with changes in bone (re-)modelling, which might be monitored non-invasively with biochemical bone markers. The aim of this study was to evaluate the influence of exercise on serum osteocalcin and serum carboxy-terminal cross-linked telopeptide of type I collagen generated by matrix metalloproteinases (CTX-MMP) concentration in young racehorses. Seventy-one 2 to 4-year-old Thoroughbreds were included in this prospective infield study. Blood sampling was performed six times (i.e. six sampling cycles) during a 9-month period. Serum samples were analysed with commercial ...
Mienaltowski MJ, Belt A, Henderson JD, Boyd TN, Marter N, Maga EA, DePeters EJ.Prophylactic supplementation of psyllium husk is recommended to enhance passage of ingested sand from the gastrointestinal tracts of horses. We hypothesized that psyllium supplementation would increase fecal sand passage and favorably alter bacterial populations in the hindgut. Six yearlings and six mature mares were fed a psyllium supplement in the diet daily for seven days. Voluntarily-voided feces were collected over the course of 29 days, prior, during, and after treatment. Feces were analyzed for acid detergent fiber (ADF) and acid detergent insoluble ash analyses. Microbial DNA was also...
Blazyczek I, Hamann H, Deegen E, Distl O, Ohnesorge B.Between 1994 and 2001, guttural pouch tympany was diagnosed in 51 foals; there were approximately three times as many fillies as colts, of Arabian, different German warmblood breeds and Western horse breeds. There were significantly more Arabian and paint horse foals than expected in comparison with the breed distribution of the foals hospitalised at the Clinic for Horses. The foals' breed and sex did not influence the age of onset, the type and severity of the clinical signs or the recurrence rate. A surgical laser technique was used on 50 of the foals; in 35 cases only one surgical treatment...
Multigner L, Magistrini M, Ducot B, Spira A.Several reports have suggested that human semen quality has declined throughout the world over the last few decades. Chemicals in the environment acting as endocrine disrupters have been implicated as a possible cause. If this is indeed the case, then similar effects may be observed in animals. We analyzed 1489 ejaculates collected from 390 Breton draught stallions between 1981 and 1996. Semen was collected from all the stallions at a single center, according to standardized semen collection protocols and laboratory methods. Semen volume decreased slightly but significantly and there was an in...
Lovsin E, Fazarinc G, Pogacnik A, Bavdek SV.A typical body format of Slovenian Lipizzan horse was investigated. The study included 6 foals (5 colts and 1 filly) at the Lipica stud farm. They were measured from birth to twenty-seven months and again at forty-four months of age. Measurements included body length, chest circumference, withers height and body mass. All those measurements were statistically evaluated and compared to some other horse breeds to determine the similarity of the growth dynamics of those horse breeds. It was concluded that the parameters of the head and neck reached their full growth at the age of 27 months and al...
Leng J, McNally S, Walton G, Swann J, Proudman C, Argo C, Emery S, La Ragione R, Eustace R.Gut microbial communities are increasingly being linked to diseases in animals and humans. Obesity and its associated diseases are a concern for horse owners and veterinarians, and there is a growing interest in the link among diet, the intestinal microbiota and metabolic disease. Objective: Assess the influence of long-term hay or haylage feeding on the microbiota and metabolomes of 20 Welsh mountain ponies. Methods: Longitudinal study. Methods: Urine, faeces and blood were collected from 20 ponies on a monthly basis over a 13-month period. Urine and faeces were analysed using proton magnetic...
Webb HJ, Weston JF, Norman EJ, Cogger N, Bolwell CF, Rogers CW.Training measures, particularly those that examine the interactions between training volume, speed, and recovery, can improve understanding of training practices that contribute to success and avoid adverse horse welfare outcomes in endurance competitions. This study describes the training of Fédération Equestre Internationale (FEI) 100-160 km level horses in New Zealand. A convenience sample of 16 participants and 25 horses were surveyed before commencement of training. Participants then reported daily data for an entire nine-month season of training and competition. Participants began the...
Peloso JG, Schumacher J, McClure SR, Crabill MR, Hanselka DV, Householder DD, Potter GD.A T-cannula was fitted permanently into the ileum of ten mature ponies to quantify the role of the prececal and postileal segments of the digestive tract in equine nutrition studies. The ponies were anesthetized, positioned in left lateral recumbency, and the distal small intestine was exteriorized through a right paralumbar incision. A silastic T-cannula was inserted through a 2-cm longitudinal incision into the lumen of the ileum, on the antimesenteric surface, and sutured to the ileum. The stem of the cannula was exteriorized through a 2-cm circular skin incision, 6 cm caudal to the 16th ri...
Krueger K, Gruentjens T, Hempel E.Wolves returned to Germany in 2000, leading to fear in German horse owners that their horses could be in danger of wolf attacks or panic-like escapes from pastures when sighting wolves. However, reports from southern European countries indicate that wolf predation on horses diminishes with increasing presence of wildlife. Therefore, we conducted a long-term, filed observation between January 2015 and July 2022 on 13 non breeding riding horses, mares and geldings, kept permanently on two pastures within the range of wildlife and a stable wolf pack with annual offspring. Wildlife cameras at the ...
de Bont MP, Proudman CJ, Archer DC.There is limited information about risk factors and survival associated with disorders of the small colon requiring surgical management. Objective: 1) To document the types of surgical lesion in horses where pathology of the small (descending) colon was the primary cause of abdominal pain, 2) to describe the short- and long-term survival of these cases and identify factors associated with survival and 3) to identify preoperative variables associated with localisation of a lesion to the small colon. Methods: Clinical data and long-term follow-up were obtained for horses that underwent explorato...
Bollinger L, Bartel A, Küper A, Weber C, Gehlen H.High elimination rates and the concern for horse welfare are important issues in endurance riding. An improved understanding of the causes of elimination could increase completion rates in this sport. We have identified pre-ride risk factors that allow an assessment of potential elimination before the ride. A longitudinal cohort study was performed among 49 healthy horses competing in the 160 km endurance ride at the 2016 World Championship of Endurance Riding in Samorin/Slovakia. Blood samples were drawn before the ride. For statistical evaluation, horses were categorized in three groups: fin...
Mendez-Angulo JL, Ernst NS, Mudge MC.In order to assess postoperative outcome in horses undergoing end-to-end anastomosis of the small intestine, performed using a one-layer technique, 15 horses that underwent exploratory coeliotomy, resection of the small intestine and end-to-end anastomosis using a continuous Lembert pattern were studied. Information on the age, breed, sex, diagnosis, treatment, complications and outcome of each case were obtained from medical records. Follow-up information was obtained via telephone conversations with clients and trainers. Five of the horses had short-term postoperative complications: one had ...
Southwood LL, Lindborg S, Myers M, Aceto HW.To compare long-term outcome of Salmonella-positive versus Salmonella-negative horses discharged from hospital after colic surgery. Methods: Retrospective case-control. Methods: Horses discharged from the hospital after colic surgery. For each horse with positive culture for Salmonella enterica (SAL-POS, n = 59), at least 2 horses testing negative for S. enterica (SAL-NEG, n = 119) were enrolled. Methods: Owners were interviewed via phone at least 12 months after surgery regarding: (1) complications after discharge from the hospital; (2) duration of survival; and (3) return to prior or...
Weijs W, Dingboom E, van Ginkel F.The contribution to total variance of different error sources in fibre type counts of equine gluteus medius muscle biopsies was determined to quantify and possibly improve the resolution of the method. Fibre types were defined on the basis of myosin heavy chain immunostaining. Errors were determined at levels: (1) positioning the insertion channel, (2) positioning the needle tip (3) biopsy heterogeneity (4) observer interpretation. Errors at levels 1 and 2 were considerable. Confidence intervals for individual observations were +/- 10-15%. In longitudinal studies a group size of 4 animals is n...
Gharbi M, Drissi G, Darghouth MA.A preliminary study was carried out to examine the population dynamics of ticks on 24 pure Arabian horses. The horses were monitored monthly over one year in the Ghardimaou region of north-west Tunisia. A total of 120 adult ticks were collected and identified, leading to the estimation of different parasitological indicators. The ticks belonged to a single genus (Hyalomma) and three species: H. scupense (59%), H. marginatum (28%) and H. excavatum (13%) (p < 0.001). The mean infestation prevalence was 29.5%; the highest prevalence was observed for H. scupense (41.7%) (p 0.05), but the propo...
Ishii H, Shibuya M, Kusano K, Sone Y, Kamiya T, Wakuno A, Ito H, Miyata K, Yamada M, Leung GN.Vadadustat is a newly launched hypoxia-inducible factor stabilizer with anti-anemia and erythropoietic effects; however, its use in horses is expressly forbidden in both racing and equestrian competitions. Following our previous report on the pharmacokinetic study of vadadustat in horse plasma and urine, a long-term longitudinal analysis of vadadustat in horse hair after nasoesophageal administration (3 g/day for 3 days) to three thoroughbred mares is described in this study. Our main objective is to further extend the detection period of vadadustat for the purpose of doping control. Three...
Rebhun WC.Corneal squamous cell carcinomas with surface areas of more than 2.0 cm were diagnosed in 26 eyes of 25 horses. The depth of the lesion into the corneal stroma and involvement of the ocular adnexa were important determinants for surgical treatment. Corneal tumors that extended into adjacent palpebral conjunctiva, eyelid, or orbit were not amenable to complete excision, while those that involved only cornea and bulbar conjunctiva were treated surgically by keratectomy and beta radiation. Initial treatment was keratectomy followed by beta radiation on 24 eyes and enucleation of two eyes. Twenty ...
Faramarzi B, Salinger A, Kaneps A, Nout-Lomas Y, Greene H, Dong F. The goal of this study was to quantify external and internal anatomical characteristics of the foal foot throughout the first year of age. Digital radiographs and photographs were taken bimonthly of the forefeet of nine Arabian foals, beginning at about 2 weeks of age until 12 months of age. Sixty-eight linear and angular variables were measured using NIH (National Institutes of Health) software. Statistical analyses were performed using piecewise random coefficient model and -values < 0.05 were considered significant. Distinct changes in hoof development were identified betwe...
Werpy N, Chapman K, Griffith L.When evaluating suspensory ligament branch splits, there is a discrepancy between high field MRI findings and weight bearing ultrasonographic imaging characteristics. In this descriptive and retrospective case series, ultrasonographic examination was performed on suspensory ligament branches with the limbs in weight bearing and non-weight bearing positions. Suspensory ligament branch splits were defined as linear regions of decreased echogenicity when imaged with the limb in a weight bearing position that increased in size and became anechoic with the limb in a non-weight bearing position. Thi...
Christophersen MT, Tnibar A, Pihl TH, Andersen PH, Ekstrøm CT.There is a paucity of studies addressing sporting activity and horse owners' satisfaction after horses have undergone colic surgery. Objective: To determine 1) survival rate after colic surgery, 2) prevalence of horses returning to, or starting, sporting activities and 3) assess the owners' satisfaction regarding colic surgery. Methods: Cases that underwent exploratory celiotomy for colic between January 2005 and August 2010 were reviewed. All horses that had one or more celiotomies and were discharged after colic surgery were included in a telephone questionnaire survey. Only horses that surv...
Madry MM, Spycher BS, Kupper J, Fuerst A, Baumgartner MR, Kraemer T, Naegeli H.Compared to blood or urine, drugs can be detected for much longer periods in the long hair of horses. The aim of this study was to establish and validate a highly sensitive liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the detection and quantification of frequently prescribed opioids, sedatives and non-steroidal anti-inflammatory agents in the mane and tail hair of horses. Based on an average growth rate of about 2 cm per month, times of administration reported by horse owners or veterinary physicians were related to drug localizations in hair. Hair samples were collecte...
Rhiner T, Fettelschoss V, Schoster A, Birkmann K, Fettelschoss-Gabriel A.Previously, virus-like particle (VLP)-based self-vaccinations targeting interleukin (IL)-5 or IL-31 have been suggested to treat equine insect bite hypersensitivity (IBH), a seasonal recurrent allergic dermatitis in horses. The IL-5-targeting equine vaccine significantly reduced blood eosinophil counts in horses, similar to human monoclonal antibodies targeting IL-5 or the IL-5 receptor alpha (IL-5Rα). Previous studies in humans have also reported an additional effect on reduction of basophil counts. The aim of the present study was to evaluate whether an equine anti-IL-5 vaccine affected blo...
Stewart AA, Rush B, Davis E.To document the efficacy of intratumoural injections of 5-fluorouracil for the treatment of equine sarcoids. Methods: A prospective study that included 13 horses and one donkey. Methods: Sarcoids were confirmed by histological examination and treated with intratumoural 5-fluorouracil every 2 weeks. If the sarcoids did not resolve after seven treatments, treatment was considered a failure. All cases were re-examined 6 months after treatment commenced and owners were telephoned 3 years after commencement of treatment to report on tumour recurrence. Outcome comparisons were performed to determine...
Plevin S, McLellan J, van Schie H, Parkin T.Injuries to the superficial digital flexor tendon (SDFT) are one of the leading causes of Thoroughbred (TB) wastage. Increasingly, the aim is to prevent injury rather than treat it. Conventional ultrasonography is not sufficiently sensitive to accurately monitor tendon and predict injury. Ultrasound tissue characterisation (UTC) is a relatively new technique, which improves tendon characterisation by providing a 3-dimensional (3D) SDFT reconstruction and objective calculation of fibre alignment by classifying fibres into one of 4 echo-types. Objective: To report a reference range of echo-types...
Vasilieva NA, Tchabovsky AV.Fitness consequences of early-life conditions remain unclear and poorly studied in mammals. Based on long-term observations of yellow ground squirrels (Spermophilus fulvus), we identified early determinants of female fitness by analyzing the effects of early-life individual and environmental characteristics (weaning weight, weight gain rate, date of natal emergence, natal litter size, location of the natal burrow, local density of juveniles, population density and precipitation in the post-weaning period) on lifetime reproductive success (LRS). We found high variation and right-skewed distribu...
